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  • vitro
  • The US National Cancer Institute (NCI) 60 human tumour cell line anticancer drug screen (NCI60) was developed in the late 1980s as an in vitro drug-discovery tool intended to supplant the use of transplantable animal tumours in anticancer drug screening. (nih.gov)
  • Dillman RO, Nayak SK, Beutel L (1993) Establishing in vitro cultures of autologous tumor cells for use in active specific immunotherapy. (springer.com)
  • in vitro , 70% of the intracellular topotecan pool was transported out of the T8 or MX3 cells within 30 s. (aacrjournals.org)
  • In vitro analysis showed that the growth of p16‑null bladder tumor cells and the hyperphosphorylation of their pRbs were inhibited by p16 transduction in a concentration‑dependent manner. (spandidos-publications.com)
  • Tumour Cell Lines HT-29 and FaDu Produce Proinflammatory Cytokines and Activate Neutrophils In Vitro: Possible Applications for Neutrophil-Based Antitumour Treatment," Mediators of Inflammation , vol. 2009, Article ID 817498, 13 pages, 2009. (hindawi.com)
  • These results demonstrate significant gene expression differences between tumor material examined unselected as part of their original microenvironment, pure populations of the melanoma cells cultured under optimal, but artificial, conditions in vitro , and grown as tumors containing mouse stromal cells. (aacrjournals.org)
  • different tumor
  • With the extensive use of cell lines and xenografts in cancer research the information obtained using our approach may help to better interpret results generated from different tumor models by understanding some common differences at the gene expression level, information that may have important practical and biological implications. (aacrjournals.org)
  • anticancer
  • The NCI60 human tumour cell line anticancer drug screen. (nih.gov)
  • We have used cancer cell lines as a model system for examining the relationship between underlying genotypes and sensitivity to candidate anticancer agents. (aacrjournals.org)
  • Cytochrome P450 (P450) enzyme expression patterns were determined for a panel of 60 human tumor cell lines, representing nine tumor tissue types, used by the National Cancer Institute (NCI) Anticancer Drug Screening Program. (aspetjournals.org)
  • Significant negative correlations between the patterns of P450-dependent 7-benzyloxyresorufin metabolism activity and cell line chemosensitivity were observed for 10 standard anticancer agents (including 6 alkylating agents) and 55 investigational compounds, suggesting a role for P450 metabolism in the inactivation of these agents. (aspetjournals.org)
  • The expression of P450 enzymes in tumor tissue can have a major impact on the responsiveness of tumors to cancer chemotherapeutic drugs, owing to the central role that these enzymes play in the metabolism of numerous clinically useful anticancer agents ( LeBlanc and Waxman, 1989 ). (aspetjournals.org)
  • Although, as noted above, cytochrome P450 enzymes contribute to the metabolism of a large number of drug substrates and can have a large impact on a drug's anticancer activity, little is presently known about the P450 activity levels present in the individual tumor cell lines that constitute the NCI panel. (aspetjournals.org)
  • glioma cell lines
  • Based on this evidence, in the present study, we further explored the long-term T3 effects on glioma tumors in relation to the degree of tumor aggressiveness and potential alterations in thyroid hormone nuclear receptor (TR) expression which may characterize different types of glioma cell lines. (hindawi.com)
  • Selective repression of YKL-40 by NF-kappaB in glioma cell lines involves recruitment of histone deacetylase-1 and -2. (labome.org)
  • tissue
  • Benefits of luciferase bioluminescence in cell tracking include penetration of tissue for non-invasive monitoring and the re-usability of the enzymatic marker. (bio-protocol.org)
  • sensitivity
  • The molecular mechanisms underlying the sensitivity of testicular germ cell tumor cell lines to cisplatin treatment seem to be multifactorial, possibly including a low capacity to remove cisplatin-DNA adducts by nucleotide excision repair (NER), and their proneness to die by apoptosis. (uio.no)
  • responsiveness
  • These results indicate that primary medulloblastomas express significant levels of TP73 isoforms, and suggest that they can modulate the survival and genotoxic responsiveness of medulloblastomas cells. (labome.org)
  • feasible
  • It provides a feasible mechanism of early tumour vascular supply which can co-exist and incorporate with later angiogenic mechanisms. (biomedsearch.com)
  • Patient-specific active immunotherapy with antigens from autologous proliferating, self-renewing tumor cells is a feasible approach. (springer.com)
  • therapies
  • Murine tumor models have been critical to advances in our knowledge of tumor physiology and for the development of effective tumor therapies. (bio-protocol.org)
  • Accumulating evidence points to the importance of genomic heterogeneity across human tumors as a critical determinant of the variable clinical response to molecularly targeted cancer therapies. (aacrjournals.org)
  • quantify
  • High-resolution magic angle spinning NMR (HR-MAS) was performed on harvested cell pellets to quantify the patterns of lipid signals. (springer.com)
  • Carcinoma
  • The phosphorylation of the nuclear enzyme DNA topoisomerase II was characterized from HeLa human cervical carcinoma cells labeled with 32Pi. (rti.org)
  • In contrast, aberrant FHIT transcripts were readily demonstrated in 6 of 7 cervical carcinoma cell lines and 17 of 25 (68%) primary cervical carcinomas. (aacrjournals.org)
  • Northern blot analyses demonstrated reduced or absent FHIT expression in the cervical carcinoma cell lines, particularly those with aberrant RT-PCR products. (aacrjournals.org)
  • correlation
  • To be able to compare results and look for correlation I will compare my algorithms with data from the NCI Almanac that has data on approximately 5000 drug combinations on the 60 cell lines in NCI-60. (biostars.org)
  • The intensity of NMR lipid signals varied greatly between cell types, and a good correlation was found between total volume of LDs and the proton NMR lipid signal intensity at 0.9 and 1.3 ppm. (springer.com)
  • genetically
  • Recently a novel mechanism, by which some genetically deregulated and aggressive tumour cells generate "micro-vascular" channels without the participation of endothelial cells and independent of angiogenesis, has been proposed. (biomedsearch.com)
  • resistance
  • Cornforth AN, Fowler AW, Carbonell DJ, Dillman RO (2011a) Resistance to the proapoptotic effects of interferon-gamma on melanoma cells used in patient-specific dendritic cell immunotherapy is associated with improved overall survival. (springer.com)
  • Furthermore, BCRP/MXR/ABCP expression levels in various partially revertant T8 cells correlated with the levels of resistance to topotecan, SN-38, and mitoxantrone, strongly suggesting BCRP/MXR/ABCP to be the transporter responsible for the enhanced efflux. (aacrjournals.org)
  • Overexpression of BCRP/MXR/ABCP , caused by exposure of the cells to MX or doxorubicin/verapamil, resulted in a resistance pattern that was different from that generally observed in the cases of P-gp or MRP1 overexpression. (aacrjournals.org)
  • Furthermore, BCRP/MXR/ABCP expression levels correlated with the levels of resistance in various partially revertant cell lines. (aacrjournals.org)
  • leukemia
  • Cladocalol, a formylated triterpene, was isolated from leaves of E. cladocalyx and showed cytotoxic effect on the myeloid leukemia cell line HL-60 [ 5 ]. (hindawi.com)
  • cancer cell
  • Adenoviral endoplasmic reticulum-targeted mda-7/interleukin-24 vector enhances human cancer cell killing. (labome.org)
  • We offer an extensive selection of total RNA from human tumors and human cancer cell lines. (clontech.com)
  • These studies have revealed the utility of large panels of cancer cell lines to capture the genomic heterogeneity of human cancer and to identify clinically relevant genotype-phenotype relationships. (aacrjournals.org)
  • Topotecan- or mitoxantrone-selected cell lines (T8 and MX3, respectively), derived from the human IGROV1 ovarian cancer cell line, were resistant to the topoisomerase I inhibitors topotecan, SN-38 (the active metabolite of irinotecan), and 9-aminocamptothecin, as well as to the topoisomerase II drug mitoxantrone. (aacrjournals.org)
  • Cells selected with TPT or MX from the human IGROV1 ovarian cancer cell line were resistant to TPT, as well as to the analogues SN-38 and 9-AC, with concomitant overexpression of BCRP/MXR/ABCP . (aacrjournals.org)
  • These findings support the involvement of ATM signaling in response to cisplatin in the testis cancer cell lines. (uio.no)
  • I'm trying to establish an analysis pipeline for whole exome seq (WES) of a cancer cell l. (biostars.org)
  • In this study, five nervous system cancer cell lines were investigated. (springer.com)
  • significantly
  • T3 had no effect on ERK activation in both cell lines but significantly increased phospho-Akt levels in 1321N1. (hindawi.com)
  • Prevalence for p16 methylation increased significantly (38% to 69%) in rat lung tumors induced by plutonium (Pu) or Pu plus cigarette smoke compared to tumors exposed induced by smoke only. (aacrjournals.org)
  • The results demonstrated that the expression of HMGN2 was significantly increased in the majority of tumor cell lines compared with periodontal ligament cells (PDLCs). (spandidos-publications.com)
  • The systemic delivery of the m‑p16 peptide using Wr‑T by cardiac injection significantly inhibited the growth of solid MBT‑2 tumors compared with the control phosphate‑buffered saline (PBS) injection. (spandidos-publications.com)
  • accumulation
  • In both resistant cell lines, decreased accumulation of topotecan and mitoxantrone was observed, caused by enhanced energy-dependent efflux of the drugs involved. (aacrjournals.org)
  • HeLa
  • Analysis of topoisomerase II immunoprecipitates from 32P-labeled HeLa cells indicated that phosphorylation of the enzyme occurred at serine residues. (rti.org)
  • MCF7
  • The highest degree of synergism with Olaparib was observed in MDA-MB-436 cells followed by MDA-MB-231, MCF7 and HCC-1937 cells. (aacrjournals.org)
  • occurs
  • Amplification of the chromosome region containing bcl -1 occurs frequently in breast cancer, squamous cell cancer, and other tumor types. (aacrjournals.org)
  • In solid tumors, oncogene activation occurs principally by either over-expression of an oncogene due to genetic amplification events, or by gain of function due to activating point mutations. (aacrjournals.org)
  • proteins
  • The high mobility group (HMG) proteins were originally isolated from the nuclei of mammalian cells and named according to their electrophoretic mobility ( 1 ). (spandidos-publications.com)
  • intracellular
  • These data suggest that IL-15 has an important role in activating an NK cell-mediated pathway that leads to the elimination of intracellular protozoans from the intestines, which is a previously unrecognized feature of NK cell function. (labome.org)
  • survival
  • Thyroid hormone action on cell growth, differentiation and survival during development may be of therapeutic relevance Methods and Results 1321N1 cell line, an astrocytoma grade II, and U87MG, a glioblastoma grade IV, were exposed for 2 and 4 days in medium deprived of T3 and in medium containing 1 nM T3. (hindawi.com)
  • Such treatment is associated with encouraging long-term survival rates, and results are superior in patients treated with DCV loaded with antigen from such cells. (springer.com)
  • Cornforth AN, Lee GJ, Fowler AW, Carbonell DJ, Dillman RO (2009) Increases in serum TARC/CCL17 levels are associated with progression-free survival in advanced melanoma patients in response to dendritic cell-based immunotherapy. (springer.com)
  • introduction
  • INTRODUCTION: Vascuologenesis is the de novo establishment of blood vessels and vascular networks from mesoderm-derived endothelial cell precursors (angioblasts). (biomedsearch.com)
  • context
  • Cornforth AN, Lee G, Dillman RO (2011c) Autologous peripheral blood mononuclear cell recognition of autologous proliferating tumor cells in the context of a patient-specific vaccine trial. (springer.com)
  • Tumor-specific oncogenomic signatures represent the first step towards therapeutically targeting activated oncogenes in the context of personalized cancer treatment. (aacrjournals.org)
  • We have generated oncogenomic signatures that reflect the natural history of a specific tumor sample, and this represents the first step towards therapeutically targeting activated oncogenes in the context of personalized cancer treatment. (aacrjournals.org)
  • specific
  • This study presents a paradigm for NF-kappaB regulation of one of its targets in a strict cell type specific manner. (labome.org)
  • We have established proliferating pure tumor cell cultures from cancer samples, followed by further expansion for patient-specific therapeutic purposes. (springer.com)
  • The problem is I'm not 100% sure how to create the networks that would represent a specific tumor cell line. (biostars.org)
  • Cell line-specific P450 enzyme patterns were observed using three other P450 substrates, 7-ethoxycoumarin, coumarin, and 7-ethoxyresorufin, each of which was metabolized at a low rate. (aspetjournals.org)
  • Results
  • RESULTS: There were observable differences in growth of the cells on laboratory plastic and collagen matrix. (biomedsearch.com)
  • Collectively, our results indicate that the combination of PM1183 and PARP inhibitors (Olaparib and BMN673) induces an artificial synthetic lethality that can be advantageously used to kill several breast cancer cells, independently from their BRCA1 status. (aacrjournals.org)
  • The results demonstrated that the expression of HMGN2 was increased in the majority of tumor cell lines, particularly MDA‑MB‑468 and THP‑1 cells, compared with PDLCs. (spandidos-publications.com)