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  • Tissue
  • A novel retrovirus, morphologically consistent with mammalian C-type retroviruses, was detected by electron microscopy in mitogen-stimulated peripheral blood mononuclear cell cultures from 163 koalas and in lymphoma tissue from 3 koalas. (asm.org)
  • germ-line
  • The idea that retroviral infections would occur in the germ-line and become inherited through countless generations by the host required a further leap in imagination. (royalsocietypublishing.org)
  • Indeed, germ-line integration has not yet been described for DNA tumour viruses, although we now know that it occasionally occurs with human herpesvirus 6 [ 9 , 10 ]. (royalsocietypublishing.org)
  • ERVs, by definition, have become incorporated into the host genome by integration into germ line cells or early embryos and are transmitted as dominant Mendelian alleles. (asm.org)
  • embryos
  • This report describes the establishment directly from normal preimplantation mouse embryos of a cell line that forms teratocarcinomas when injected into mice. (pnas.org)
  • Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). (abcam.com)
  • differentiation of ES cells
  • Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). (abcam.com)
  • Thus, Wnt/β-catenin signaling exhibits developmental stage-specific, biphasic, and antagonistic effects on cardiogenesis and hematopoiesis/vasculogenesis, and appropriate modulation of Wnt signaling enables highly efficient cardiomyocyte differentiation of ES cells. (pnas.org)
  • melanoma
  • Excised melanoma biopsies were partitioned and one part was immediately snap frozen in liquid nitrogen and stored at −80°C until RNA extraction, whereas the other part was used for immunohistochemistry and for the establishment of melanoma cells lines. (aacrjournals.org)
  • Human melanoma and Chinese hamster ovary cells galactosylate n-alkyl-beta-glucosides using UDP gal:GlcNAc beta 1,4 galactosyltransferase. (nih.gov)
  • viral
  • For small DNA tumour viruses, the full replication cycle occurs via non-integrated circular viral genomes, whereas viral integration into host DNA usually leads to abortive infection and sometimes to cell transformation. (royalsocietypublishing.org)
  • PCR amplified provirus from the blood and tissues of 17 wild and captive koalas, and reverse transcriptase-PCR demonstrated viral mRNA, viral genomic RNA, and reverse transcriptase activity in koala serum and cell culture supernatants. (asm.org)
  • tissues
  • Swaroop A, Xu J. cDNA libraries from human tissues and cell lines. (atcc.org)
  • Type C retrovirus-like particles were seen by transmission electron microscopy (TEM) in tissues from a leukemic koala ( 8 ), in the blood of captive koalas abroad ( 61 ), and in mitogen-stimulated peripheral blood mononuclear cells (PBMCs) derived from 18 koalas of mixed clinical status ( 46 ). (asm.org)
  • somatic
  • ORF1p has also been detected in somatic cells (syncytiotrophoblasts from placenta) of adult mice (39) and in different regions of the brain of L1-transgenic mice (40). (exposed-skin-care.net)
  • monolayer
  • The recognition of the importance of Rous's discovery after such a long delay was largely owing to the cell transformation assay in monolayer culture of chick embryo fibroblasts reported by Temin & Rubin [ 7 ] in 1958 which enabled quantitative experimental studies of virus replication and cell transformation. (royalsocietypublishing.org)
  • protein
  • Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). (abcam.com)
  • Drubin, D.G., Kirschner, M.W. "Tau Protein Function in Living Cells" J. Cell. (springer.com)
  • This line expressed neuroectodermal antigens such as A2B5, HNK-1 and NC-1, NSE, S-100 protein and vimentin. (nii.ac.jp)
  • induces
  • In contrast, the Wingless (Wg)/armadillo pathway (which corresponds to the Wnt/β-catenin pathway in vertebrates) promotes heart formation in Drosophila ( 8 , 9 ), and Wnt/β-catenin signaling induces cardiomyocyte differentiation in a mouse embryonal carcinoma cell line P19CL6 ( 10 , 11 ). (pnas.org)
  • culture
  • Cells (106 cells/mL) were starved for 12 h in their respective culture media containing 0.5 % BSA in an incubator and afterwards stimulated for 5 min with 1,25-dihydroxyvitamin D3 at various concentrations (0.1-100 nM) or with vehicle (DMSO) only. (figshare.com)