Cell line genome. Medical search. Frequent questions

Using a genome-scale, lentivirally delivered shRNA library, we performed massively parallel pooled shRNA screens in 216 cancer cell lines to identify genes that are required for cell proliferation and/or viability. (nih.gov)
Cell line dependencies on 11,000 genes were interrogated by 5 shRNAs per gene. (nih.gov)
Ubigene has modified over 5000 genes from more than 200 cell lines with our exclusive innovation CRISPR-U™ technology. (ubigene.us)
Ubigene exclusive KO Cell Line Bank, over 5000 KO cell lines, covering thousands of genes from 8 popular signaling pathways and nearly 100 diseases. (ubigene.us)
By using CRISPR-Cas to disrupt multiple genes and thereby suppress the production of HCPs, the researchers achieved a significant decrease in purification demands across the CHO cell lines they investigated. (ubigene.us)
Genome-wide loss-of-function screening is a powerful approach to discover genes and pathways that underlie biological processes. (sigmaaldrich.com)
To help you unravel your genes and pathways of interest, we collaborated with The Wellcome Sanger Institute to make the first arrayed lentiviral CRISPR knockout libraries for human and mouse genomes. (sigmaaldrich.com)
1 Covering over 17,000 human and over 20,000 mouse genes with two optimized gRNAs for each of them, our Sanger Whole Genome Arrayed Libraries are powerful tools that can help you make the next exciting discovery. (sigmaaldrich.com)
Mapping all miRNAs to the tammar genome and comparing target genes among tammar, mouse and human, we identified 163 conserved target genes. (biomedcentral.com)
In the present study, we tested TALEN and double-nicking CRISPR/Cas9 on GS cells, targeting Rosa26 and Stra8 loci as representative genes dispensable and indispensable in spermatogenesis, respectively. (cyberleninka.org)
In fact, this was shown to be possible by the transfection of genes into GS cells (Kanatsu-Shinohara et al. (cyberleninka.org)
A CRISPR/Cas9 genome editing pipeline in the EndoC-βH1 cell line to study genes implicated in beta cell function. (ox.ac.uk)
In vitro gene-knockout (KO) models to study T2D risk genes have so far focused on rodent beta cells. (ox.ac.uk)
Overall, this strategy to efficiently create stable KO in the human beta cell line EndoC-βH1 will allow for a better understanding of genes involved in beta cell dysfunction, their underlying functional mechanisms and T2D pathogenesis. (ox.ac.uk)
They fully sequenced the genome of a female Chinese hamster to map 24,044 genes, and determined the function of 82% of those genes. (springernature.app)
The researchers then compared the hamster genome to six common CHO cell lines, and found significant variability between them with over 3.7 million single base variations, over 500,000 base insertions or deletions, and over 7,000 variations in the number of copies of genes on a chromosome. (springernature.app)
An understanding of variation in apoptotic genes would help increase the longevity of CHO cells by cell-engineering. (springernature.app)
Analysis of highly metastatic melanoma cells using oligonucleotide arrays has identified genes regulating tumor invasiveness. (biomedcentral.com)
It is expected that the genes involved include those that regulate cell adhesion, cell migration and invasiveness. (biomedcentral.com)
SWCNT-, MWCNT-, crocidolite asbestos (ASB)-, ultrafine carbon black (UFCB)-treated and control SAECs genome expression signatures were subjected to comparative marker and class neighbor analyses to identify genes with highly correlated expression for each treatment. (cdc.gov)
With roughly 30,000 genes in mammalian genomes, fection with a vector encoding MyoD (Tapscott et al. (lu.se)
explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)
Transposable elements have the unique ability to move within our DNA, causing or reversing mutations, altering genes, and even affecting the size of our genome. (lu.se)

When combined with genomic characterization of these cell lines, this dataset facilitates the linkage of genetic dependencies with specific cellular contexts (e.g., gene mutations or cell lineage). (nih.gov)
The study of human populations has been at the forefront of genomic and clinical research since the Human Genome Project (HGP) was completed. (wikipedia.org)
Using this method, we examined the genomic positions of 6mA in bacteria ( E. coli ), green algae ( C. reinhardtii ), and mammalian cells (HEK239T, Huh7, and HeLa cells). (nature.com)
To that end, we mapped genomic, transcriptional and proteomic profiles for 30 breast cancer cell lines onto a curated Pathway Logic symbolic systems model of EgfR-MAPK signaling. (biomedcentral.com)
Scientists in the Broad community are sequencing and analyzing the genomes of a wide range of insects and microorganisms to understand their genetic regulation, population variation, and specialized genomic mechanisms. (broadinstitute.org)
Whole-genome sequencing (WGS) has the advantage of being able to detect structural variants at a genomic scale, but it is challenging to assess whether a fusion event found purely at the DNA level has the potential to be processed into a functional transcript. (biomedcentral.com)
PG2 integrates genome and transcriptome sequencing to incorporate protein variants containing amino acid substitutions, insertions, and deletions, as well as non-canonical reading frames, exons, and other variants caused by genomic and transcriptomic variation. (biorxiv.org)
We benchmarked PG2 using synthetic data and genomic, transcriptomic, and proteomic analysis of human leukemia cells. (biorxiv.org)
Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome. (springernature.app)
PCR on genomic DNA of Huh7 cells exposed to BNT162b2 amplified the DNA sequence unique to BNT162b2. (lu.se)

Projects similar to HGDP are the 1000 Genomes Project and the HapMap Project. (wikipedia.org)
This RNA sequencing data set of 465 human lymphoblastoid cell line samples from the CEU, FIN, GBR, TSI and YRI populations from the 1000 Genomes sample collection was created by the Geuvadis consortium (www.geuvadis.org, http://www.geuvadis.org/web/geuvadis/our-rnaseq-project). (refine.bio)

The HGDP-CEPH Human Genome Diversity Cell Line Panel is a resource of 1,063 cultured lymphoblastoid cell lines (LCLs) from 1,050 individuals in 52 world populations, banked at the Fondation Jean Dausset-CEPH in Paris. (wikipedia.org)
To date most DAE studies have been performed on EBV transformed lymphoblastoid cell lines (LCLs). (springer.com)

Our results imply that rare 6mA in the mammalian genome is introduced by RNA m 6 A machinery via a non-targeted mechanism. (nature.com)
Therefore the contribution of non-retroviral RNA viruses to evolution of the mammalian genome might be greater than previously believed. (virology.ws)

Cells, such as Chinese hamster ovary (CHO) cells, are cultured and used to produce many leading drugs," explained Nathan E. Lewis, PhD, senior author of the current study and co-director of the CHO Systems Biology Center at UCSD. (ubigene.us)
In 2012, the Novo Nordisk Foundation awarded a large grant, which has funded groundbreaking work in genomics, systems biology, and large-scale genome editing for research and technology development of CHO cells at the DTU and UCSD. (ubigene.us)
Recent advances in nucleic acid sequencing now permit rapid and genome-scale analysis of genetic variation and transcription, enabling population-scale studies of human biology, disease, and diverse organisms. (biorxiv.org)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)

The lab of Youyang Zhao, PhD , from Stanley Manne Children's Research Institute at Ann & Robert H. Lurie Children's Hospital of Chicago developed a unique nanoparticle to deliver genome editing technology, including CRISPR/Cas9, to endothelial cells, which are cells that line blood vessel walls. (eurekalert.org)
This is the first time that vascular endothelial cells could be reached for genome editing, since the usual way to deliver CRISPR/Cas9 - through a virus - does not work for this cell type. (eurekalert.org)
BFP expression in A549-Cas9-Blast stable cells infected with arrayed CRISPR lenti-gRNA viruses, seven days post infection, 20X magnification. (sigmaaldrich.com)
Harvested GS cell colonies showed a high targeting efficiency with both TALEN and CRISPR/Cas9. (cyberleninka.org)
TALEN and CRISPR/Cas9, when applied in GS cells, will be valuable tools in the study of spermatogenesis and for revealing the genetic mechanism of spermatogenic failure. (cyberleninka.org)
With that in mind, we have developed a robust pipeline to create a stable CRISPR/Cas9 KO in an authentic human beta cell line (EndoC-βH1). (ox.ac.uk)
A stable AEG‑1‑deficient HT22 neuronal cell line was constructed using CRISPR/Cas9 gene‑editing technology. (spandidos-publications.com)
2014). Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. (cdc.gov)

MAGOHB is the top gene dependency in cells with hemizygous MAGOH deletion, a pervasive genetic event that frequently occurs due to chromosome 1p loss. (nih.gov)
Small RNAs play important roles in many aspects of pre- and post-transcriptional gene regulation, epigenetic modifications, chromosome segregation and genome structure. (biomedcentral.com)
Variations in the Y chromosome can be used to explore ancestry in the direct male line. (medlineplus.gov)
Genealogists use this type of test because Y chromosome and mitochondrial DNA test results, which represent only single ancestral lines, do not capture the overall ancestral background of an individual. (medlineplus.gov)
New features include: * Improved plotting, including a new across assays plot of regions of allelic imbalance for each chromosome and whole genome plots for each assay. (lu.se)

It was considerably faster and more sensitive than using BreakDancer and Manta, software that is instead designed to detect many different types of structural variants on a genome-wide scale. (biomedcentral.com)
The authors began their study by using repeated rounds of in vivo selection to generate metastatic variants of melanoma cells: amelanotic A375P human cells and the B16F0 mouse cell line. (biomedcentral.com)
The generation of metastatic variants by selection in vivo offers an attractive experimental model, as any differences relative to parental lines may be related to metastatic capacity. (biomedcentral.com)
To date, the agnostic search of the genome by genome-wide association (GWA) to identify common variants associated with LDD has not been fruitful. (bmj.com)

All the cell lines were screened using standardized conditions to best assess differential genetic dependencies across cell lines. (nih.gov)
The whole genome sequencing and analysis of the HGDP was published in 2020, creating a comprehensive resource of genetic variation from underrepresented human populations and illuminating patterns of genetic variation, demographic history and introgression of modern humans with Neanderthals and Denisovans. (wikipedia.org)
Cancer is a heterogeneous disease resulting from the accumulation of genetic defects that negatively impact control of cell division, motility, adhesion and apoptosis. (biomedcentral.com)
2004). In order to make GS cells more widely applicable for the study of spermatogenesis at the genetic and molecular levels, it is desirable to be able to modify their genome without disturbing their spermato-genic ability. (cyberleninka.org)
In cancer, gene fusions occur frequently due to the genetic instability of cancer cells. (biomedcentral.com)
Without a system of sharing information about the genetic data being gathered on cell lines, researchers will find it difficult to select a particular cell line and culture conditions suited to their specific needs. (springernature.app)
To document the genetic characteristics of CHO, an international team of researchers, including Jun Wang of the King Abdulaziz University, Jeddah, set out to draft a reference genome to which the genomes of the various CHO cell lines can be compared, publishing their results in Nature Biotechnology . (springernature.app)
Mouse line cryopreservation is efficiently performed by sperm cryopreservation, except for mouse models containing several modified alleles which benefit from embryo cryopreservation to maintain their genetic composition. (cinj.org)
Rational genetic engineering to enhance biotherapeutic proteins has become a reality catalyzed by publication of the genome sequences of multiple Chinese hamster ovary (CHO) cell lines. (bioprocessintl.com)
Desirable attributes of a CHO cell line include its ability to produce high titers of biotherapeutic proteins facilitate quick recoveries and selection processes maintain phenotypic and genetic stability throughout in-vitro aging of a culture. (bioprocessintl.com)
prevention by elucidating the molecular etiology and prevention), using in vitro mechanisms by which genetic and models and state-of-the-art approaches The expected outcome of these studies epigenetic alterations alter critical including (epi)genome-wide screens is the opening up of an opportunity molecular pathways and promote cancer and functional genomics. (who.int)
Johan Jakobsson, a professor at Lund University and research group leader at MultiPark and Lund Stem Cell Center, explains, "LINE-1 retrotransposons are a rich source of genetic sequences that we suspect have shaped the evolution of the human brain, and we now have the tools to explore their role in brain development. (lu.se)
There must be something else that affects how the original genetic code in the DNA sequence is read and what message is sent into the cells. (lu.se)
Mattias Höglund and his staff have drawn up a classification system for bladder cancer based on information on genetic mutations and changes in gene expression in both the genome and the epigenome. (lu.se)

Characterization of La Crosse virus small genome transcripts. (microbiologyresearch.org)

Dr. Zhao explained that genome editing in endothelial cells could even treat cancers by cutting off the blood supply to the tumor or blocking cancer metastasis. (eurekalert.org)
Furthermore, POGLUT2 knockdown of breast cancer cells was established, followed by in vitro biological function assays and in vivo tumor growth study. (frontiersin.org)
POGLUT2 was mainly expressed in stromal cells as verified by StromalScore, ESTIMATEScore, ImmuneScore, and Tumor purity, and POGLUT2 was positively correlated with cancer-associated fibroblasts, macrophages, monocytes, and neutrophils in the tumor microenvironment. (frontiersin.org)
Increased levels of POGLUT2 could result in the dysregulated immune cell infiltration and tumor microenvironment and showed a significant regulatory effect on the progression of breast cancer through Notch-related signaling. (frontiersin.org)
Metastasis, the ability of cancer cells to leave the primary tumor site and spread throughout the body, presents the greatest clinical threat to the survival of cancer patients. (biomedcentral.com)
Poorly metastatic tumor cell populations are injected into the tail vein and relatively rare cells that are capable of metastasizing to the lungs are dissected several weeks later. (biomedcentral.com)
Future plans === * Adaptation of BAFsegmentation to samples having 100% tumor content, for example, cell-lines. (lu.se)
Infinium data for four matched tumor-normal pairs and a dilution series of a tumor cell line mixed with its paired normal cell line are available in NCBI's Gene Expression Omnibus with accession [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11976 GSE11976]. (lu.se)
The Infinium data for the dilution series of a tumor cell line mixed with its paired normal cell line are also available as a [http://cbbp.thep.lu.se/~markus/software/BAFsegmentation/CRL2324_dilutionSeries_BeadStudio.zip?format=raw BeadStudio project] and as a [http://cbbp.thep.lu.se/~markus/software/BAFsegmentation/CRL2324_dilutionSeries_TableExport.zip?format=raw tab-delimited text file] exported from Beadstudio. (lu.se)

And recent genome-wide analyses showed that major transcriptional regulators governing development are under the regulation of PcG complexes. (karger.com)
The mitogen-activated protein kinase (MAPK)/Erk pathway plays a central role in cell communication: it orchestrates signaling from external receptors to internal transcriptional machinery, which leads to changes in phenotype [ 6 , 7 ]. (biomedcentral.com)
In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)

Bunyavirus RNA synthesis: genome transcription and replication. (microbiologyresearch.org)
Arenaviruses have an RNA genome which is not known to be copied into DNA at any stage of the replication cycle. (virology.ws)
The RNA genome of retroviruses is converted to a DNA form during viral replication by the viral enzyme reverse transcriptase. (virology.ws)
Sir,I guess there might be retrotransposon - independent integration in other dsRNA viruses,but after integration we fail to find out the virus RNA genome,as during host genome replication virus RNA genome will be replaced by the DNA from DNA polymerases.Retrotransoson mediated RNA virome integration may not be universal. (virology.ws)
The first is lytic infection, which occurs when an adenovirus enters human epithelial cells and continues through an entire replication cycle, which results in cytolysis, cytokine production, and induction of host inflammatory response. (medscape.com)

Compared to LC-MS/MS, sequencing-based methods identify modified bases affiliated with their sequence context, which facilitates a comprehensive genome-wide analysis along with downstream functional studies 28 . (nature.com)
Whole-genome sequence of the Spodoptera frugiperda Sf9 insect cell line. (pacb.com)
The draft whole-genome sequence of the Spodoptera frugiperda Sf9 insect cell line was obtained using long-read PacBio sequence technology and Canu assembly. (pacb.com)
Its goal was to release an annotated assembly from 10X genome sequence coverage for Batrachochytrium dendrobatidis diploid strain JEL423, which was provided by Dr. Joyce Longcore at The University of Maine. (broadinstitute.org)
This basal position increases the value of a whole genome sequence for comparative genomics within the fungal clade and also with the sister animal clade. (broadinstitute.org)
strain RB-44 complete genome sequence using PacBio single-molecule real-time sequencing technology. (genomeweb.com)
Full genome virus detection in fecal samples using sensitive nucleic acid preparation, deep sequencing, and a novel iterative sequence classification algorithm. (genomeweb.com)
They then used polymerase chain reaction to isolate LCMV DNA from the cloned cells, including the cellular sequence flanking the viral genome. (virology.ws)
Nucleotide sequence analysis revealed that both cell lines contain a copy of the viral glycoprotein (GP) gene joined to cellular sequences encoding an IAP (intracisternal A type) retrotransposon. (virology.ws)

This study encompasses the first analyses of the major classes of small RNAs for the newly completed tammar genome, validates preliminary annotations using deep sequencing and computational approaches, and provides a foundation for future work on tammar-specific as well as conserved, but previously unknown small RNA progenitors and targets identified herein. (biomedcentral.com)

Expression, gene mutation and amplification, methylation, and copy number alteration (CNA) of POGLUT2 were evaluated using The Cancer Genome Atlas (TCGA), Cancer Cell Line Encyclopedia (CCLE), and Genotype-Tissue Expression (GTEx) databases. (frontiersin.org)
To address this aim we have designed the lungNENomics study, an international cohort of over 250 cases of pulmonary carcinoids, with clinical data and central pathology review, as well as whole-genome sequencing, RNA sequencing, DNA methylation array, and digital spatial profiling data. (who.int)

Broad Genomics Platform sequences a whole human genome every four minutes. (broadinstitute.org)

We describe a method for validation of fusion transcripts detected by RNA-Seq in matched whole-genome sequencing (WGS) data. (biomedcentral.com)

Using advanced sequencing technologies and a set of specialized bioinformatics tools, created by PhD student Raquel Garza, the researchers successfully detected the activity of LINE-1 elements and were able to dig into their functions and epigenetic profiles. (lu.se)

Although the cultured SSCs, named germline stem (GS) cells, are targets of genome modification, this process remains technically difficult. (cyberleninka.org)

Boyer LA, Plath K, Zeitlinger J, Brambrink T, Medeiros LA, et al: Polycomb complexes repress developmental regulators in murine embryonic stem cells. (karger.com)
Robust F1 hybrid embryonic stem cell line from Mus musculus (lab mouse), generated by crossing C56BL/6J mice to Black-Swiss mice. (seattlechildrens.org)
We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)

This approach helped us to address these highly repetitive sequences, usually masked in standard bioinformatics pipelines, allowing us to accurately measure LINE-1 expression in each cell type found in our samples," explains Raquel Garza, co-first author of the study. (lu.se)

Our exclusive Sanger CRISPR library provides extensive, high-quality gene knockout, so you can fast-track your research with the first whole genome arrayed library. (sigmaaldrich.com)
RNA Sanger sequencing analysis was performed in AEG‑1‑deficient HT22 cells and wild‑type HT22 cells without knockout (KO). (spandidos-publications.com)

Mass spectrometry-based proteomics now enables genome-scale measurements of proteomes from diverse cells, tissues, and organisms. (biorxiv.org)
Increased levels of POGLUT2 were found in multiple types of cancer tissues and cell lines. (frontiersin.org)
These primary cell cultures were often noted to spontaneously degenerate over time, and adenoviruses are now known to be a common cause of asymptomatic respiratory tract infection that produces in vitro cytolysis in these tissues. (medscape.com)
The challenge has been to determine what these elements do and how they affect human tissues, given their abundant and repetitive presence in the human genome. (lu.se)

DNMT3A is a critical regulator of hematopoietic stem cell (HSC) differentiation and somatic DNMT3A mutations are frequent in hematologic malignancies and clonal hematopoiesis. (haematologica.org)
This method improves upon and allows for the use of CRISPR technology to modify a single allele in cases where modification of both alleles produce deleterious mutations, which could adversely affect the survival of the cell/embryo or alter stem cell properties through sustained DNA damage response. (cinj.org)

Characterization of the 5′ and 3′ ends of the viral messenger RNAs isolated from BHK-21 cells infected with Germiston virus (Bunyavirus). (microbiologyresearch.org)
The L protein of Rift Valley fever virus can rescue viral ribonucleoproteins and transcribe synthetic genome-like RNA molecules. (microbiologyresearch.org)
The viral DNA then integrates into the host's genome, becoming a permanent part of the cell. (virology.ws)
Of interest is the role of adenoviruses as viral vectors in vaccination and in gene therapy.For example, in viral vector vaccines against SARS-CoV2, the vector virus is used to deliver RNA encoding SARS-CoV2 spike protein into target cells. (medscape.com)

Phylogenetic studies based on rDNA and on whole mitochondrial genome sequences indicate that the chytrids are basal in the fungal clade (3,7). (broadinstitute.org)

Small RNAs have proven to be essential regulatory molecules encoded within eukaryotic genomes. (biomedcentral.com)
These short RNAs participate in a diverse array of cellular processes including gene regulation, chromatin dynamics and genome defense. (biomedcentral.com)
As part of the genome sequencing initiative for the tammar, we have explored the evolution of each of the major classes of mammalian small RNAs in an Australian marsupial for the first time, including the first genome-scale analysis of the newest class of small RNAs, centromere repeat associated short interacting RNAs (crasiRNAs). (biomedcentral.com)

In addition, it is possible that RNA virus vectors used for gene therapy could integrate into the human genome via the mechanism described in this study. (virology.ws)
Therefore the ability of specific RNA virus vectors to integrate into the human genome should be carefully tested before the vectors are approved for use in humans. (virology.ws)
Suicide gene systems that convert nontoxic systemically delivered prodrugs to active chemotherapeutic agents have been delivered via adenoviral vectors directly into cancer cells. (medscape.com)

Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)

We have reported that the expression of BMI1 , a member of PcG, in hematopoietic stem cells or progenitor cells predicts the prognosis of patients with MDS and progression to acute leukemia. (karger.com)

The Sanger library is the only tool that allows both whole genome CRISPR KO interrogation and sophisticated phenotypic readout in a convenient arrayed format. (sigmaaldrich.com)

The nanoparticle we developed is a powerful new delivery system for genome editing in vascular endothelial cells, and could be used to treat many diseases, including acute respiratory distress syndrome from severe COVID-19," said senior author Dr. Zhao from Lurie Children's. (eurekalert.org)
Our nanoparticle delivery system for genome editing and transgene expression also is a huge advance for cardiovascular research," added Dr. Zhao. (eurekalert.org)
At the same time, we already provide customers with high quality gene-editing tools for cell or animal research worldwide. (ubigene.us)
We are committed to providing unmatched manufacturing and support for genome editing technology. (sigmaaldrich.com)
Quantifying genome-editing outcomes at endogenous loci with SMRT sequencing. (genomeweb.com)
A study in Nature suggests epitope editing in donor stem cells prior to bone marrow transplants can stave off toxicity when targeting acute myeloid leukemia with immunotherapy. (genomeweb.com)
Cell line editing provides investigators with custom-modified KO or KI cell lines. (cinj.org)

NEUROD1-KD, however, only had a modest elevation, by 34%, in the pro-apoptotic transcription factor CHOP and a gene expression profile indicative of chronic ER stress without evidence of elevated cell death. (ox.ac.uk)
On the other hand, SLC30A8-KO cells demonstrated no reduction in K ATP channel gene expression in contrast to siRNA silencing. (ox.ac.uk)
We compared the distribution of allelic ratios of gene expression in fresh blood (B cells and total mononuclear cells), transformed lymphoblasts, and breast tissue from unmatched healthy individuals. (springer.com)
We detected high levels of BNT162b2 in Huh7 cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is an endogenous reverse transcriptase. (lu.se)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)

Cell 111:185-196 (2002). (karger.com)

They showed that overexpression of RhoC in the parental A375P cells dramatically increased metastasis in vivo and cell migration in vitro . (biomedcentral.com)
In this study, we investigated the effect of BNT162b2 on the human liver cell line Huh7 in vitro. (lu.se)
Here, we use our subchronic in vitro exposure model coupled with toxicogenomic profiling and correlation feature selection strategies to identify particle-specific, key gene markers from our CNT-transformed cell lines. (cdc.gov)
Here, we present robust SWCNT-, MWCNT-, ASB- and UFCB-induced specific gene marker sets for in vitro chronically exposed lung epithelial cells. (cdc.gov)

In addition, the ErbB receptors integrate a diverse array of signals, both at the cell surface level and through cross-talk with other pathways, such as the phosphoinositide 3-kinase (Pi3k) pathway [ 11 ]. (biomedcentral.com)

The cultured SSCs, named germline stem (GS) cells, are now useful in research on various aspects of spermatogenesis. (cyberleninka.org)
This ES cell line does not require feeder cells for propagation and readily transmits through the germline using outbred CD1 4-8 cell embryos as hosts. (seattlechildrens.org)
Although good quality ES cells can provide chimeras, we do not guarantee germline transmission. (lu.se)

NEUROD1-KO cells were not viable and displayed elevated markers for ER stress and apoptosis. (ox.ac.uk)
The hamster genome was also used as a reference to study apoptosis - programmed cell death - in CHO cells, a major limitation to their durability in industrial settings. (springernature.app)

Bioprocessing begins upstream, most often with culturing of animal or microbial cells in a range of vessel types (such as bags or stirred tanks) using different controlled feeding, aerating, and process strategies. (bioprocessintl.com)

AEG-1 has been shown to localize to the cell membrane, cytoplasm, endoplasmic reticulum, nucleolus and nucleus in cancer cells ( 2 ). (spandidos-publications.com)
Immunohistochemistry using antibody binding to LINE-1 open reading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicated increased nucleus distribution of LINE-1. (lu.se)

A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
The 2024 edition of the Stem Cell Stars seminar series will explore diverse topics from the dynamic processes of cell differentiation to the latest advances in stem cell engineering. (lu.se)

Retrotransposons are sequences related to retroviruses that are found in the genome of many organisms. (virology.ws)
They are amazingly abundant: 42% of the human genome is made up of retrotransposons. (virology.ws)
Their latest investigations have led them to a specific family of transposable elements known as LINE-1 retrotransposons, which currently make up roughly 17% of our DNA. (lu.se)

B19 is the primary etiologic agent causing TAC in patients with chronic hemolytic anemias (e.g., sickle cell disease, hemoglobin SC disease, hereditary spherocytosis, alpha-thalassemia, and autoimmune hemolytic anemia) (22,23). (cdc.gov)

However, there are important structural and functional differences between rodent and human beta cell lines. (ox.ac.uk)
They discovered that LINE-1 elements play an important role in shaping the structural and functional complexity of our brains as we age. (lu.se)

perhaps the levels of expression of those in parental lines is already sufficient. (biomedcentral.com)
Our results indicate a fast up-take of BNT162b2 into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. (lu.se)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)

In contrast to bacteria and green algae, human cells possess a very limited number of 6mA sites which are sporadically distributed across the genome of different cell types. (nature.com)
Isolation and culture were crucial, as all emerging bacteria have been grown on artificial media or cell lines or at least propagated in animals. (cdc.gov)

Cell lines derived from the ovaries of Chinese hamster (CHO) are widely used to produce proteins and other biological pharmaceuticals. (springernature.app)
Development of a safe and high-quality Chinese hamster ovary (CHO) cell line is of paramount importance for the chemistry, manufacturing, and controls (CMC) portion of studies that support investigational new drug (IND) applications (1, 2). (bioprocessintl.com)

With the exception of completely de novo mass spectrometry identification algorithms which currently lack the requisite accuracy and efficiency for genome-scale studies, all current methods depend on matching observed mass spectra to reference proteome databases. (biorxiv.org)
Reverse transcription‑quantitative PCR and western blotting were carried out to analyze the knockdown efficiency of AEG‑1‑deficient HT22 cell line. (spandidos-publications.com)

Improved hybrid genome assemblies of two strains of Bacteroides xylanisolvens, SD_CC_1b and SD_CC_2a, obtained using Illumina and 454 sequencing technologies. (genomeweb.com)

Recently, evidence has been accumulating on the molecular mechanism underlying self-renewal of stem cells. (karger.com)
There is emerging evidence indicating that the PcG complexes are indispensable for sustaining stem cell activity and cancer stem cells. (karger.com)
Bruggeman SWM, Valk-Lingbeek ME, van der Stoop PPM, Jacobs JJL, Kieboom K, et al: Ink4A and Arf differentially affect cell proliferation and neural stem cell self-renewal in Bmi1-deficient mice. (karger.com)
Summary Mouse spermatogonial stem cells (SSCs) can be cultured for multiplication and maintained for long periods while preserving their spermatogenic ability. (cyberleninka.org)
Spermatogonial stem cells (SSCs) are pivotal for maintaining the lifelong sperm production of mammalian males. (cyberleninka.org)
The nature of the stem cell substates and their relationship to commitment to differ- entiate and lineage selection can be elucidated in terms of a landscape picture in which stable states can be defined mathematically as attractors. (lu.se)
The biological properties and clinical potential of stem cells elicit that are generated must not be unduly sensitive to small fluctu- continued scientific, commercial, and public interest. (lu.se)
To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)
Welcome to the Lund Stem Cell Center's, Stem Cell Stars seminar series! (lu.se)
A new seminar series featuring some of the brightest minds in stem cell science and regenerative medicine from around the world. (lu.se)
Immerse yourself in groundbreaking insights and discoveries, engage in captivating discussions, gain fresh perspectives, and network with fellow scientists and Stem Cell Center members during our post-seminar mingling sessions. (lu.se)
Get ready for an exciting start to our 2024 Stem Cell Stars series! (lu.se)

The RNA genomes of these viruses are copied via an RNA intermediate, entirely in the cytoplasm of the cell. (virology.ws)

2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)

The Batrachochytrium dendrobatidis sequencing project is part of the Broad Institute Fungal Genome Initiative. (broadinstitute.org)

Anatomy26

Organisms12

Diseases21

Chemicals and Drugs47

Analytical, Diagnostic and Therapeutic Techniques and Equipment37

Phenomena and Processes114

Disciplines and Occupations4

Technology, Industry, Agriculture1

Information Science11

Health Care1

Genes23

Genomic10

10002

Lymphoblastoid cell lines2

Mammalian genome2

Biology4

Cas98

Chromosome5

Variants4

Genetic14

Small genome1

Tumor9

Transcriptional3

Replication5

Sequence9

Annotations1

Methylation2

Genomics1

Transcripts1

Epigenetic1

Targets1

Embryonic3

Bioinformatics1

Knockout2

Tissues4

Mutations2

Viral4

Mitochondrial1

RNAs3

Vectors3

Populations1

Acute leukemia1

Sanger1

Editing7

Gene expression5

20021

Vitro4

Pathways1

Germline3

Apoptosis2

Microbial1

Nucleus2

Differentiation3

Retrotransposons3

Sickle Cell Di1

Functional2

Expression3

Bacteria2

Hamster2

Efficiency2

Strains1

Stem12

Viruses1

Transcription1

Initiative1