• Precursor cells and their phenotypes during the differentiation of hematopoietic stem cells towards immature B cells. (uni-potsdam.de)
  • Robust F1 hybrid embryonic stem cell line from Mus musculus (lab mouse), generated by crossing C56BL/6J mice to Black-Swiss mice. (seattlechildrens.org)
  • Researchers make hiPSCs by harvesting somatic cells-often from skin-from a person and then reprogramming them to enter an embryonic-like state. (the-scientist.com)
  • We tested 273 hepatotoxic, 191 cardiotoxic, and 85 nephrotoxic compounds in HepG2 (hepatocellular carcinoma), H9c2 (embryonic myocardium), and NRK-52E (kidney proximal tubule) cells for their cytotoxicity. (nih.gov)
  • Researchers at Johns Hopkins have established a human cell-based system for studying sickle cell anemia by reprogramming somatic cells to an embryonic stem cell like state. (hopkinsmedicine.org)
  • The research team first sought to improve previously established methods for reprogramming of adult cells into so-called induced pluripotent stem (iPS) cells, which look and behave similarly to embryonic stem cells and can differentiate into many different cell types. (hopkinsmedicine.org)
  • Without large T, cells form embryonic stem cell-like clusters in three to four weeks. (hopkinsmedicine.org)
  • With large T, the cells started looking like embryonic stem cells in just 12 to 14 days. (hopkinsmedicine.org)
  • Not only did T speed up reprogramming, we also found that it increases the total number of reprogrammed cells, which is great because often in reprogramming, not all cells go all the way," says Cheng, who explains that rigorous follow-up tests are required to determine if the reprogrammed cells really behave like pluripotent embryonic stem cells. (hopkinsmedicine.org)
  • Embryonic stem cell-like clusters were visible 14 days after they initiated reprogramming and from these clusters the researchers established three different cell lines that both look and behave like human embryonic stem cells. (hopkinsmedicine.org)
  • On August 9, 2001, President Bush announced that only embryonic stem cell lines created before that date could be used in federally funded research. (ca.gov)
  • Martin Pera has a CIRM grant to develop techniques for creating high quality new human embryonic stem cell lines. (ca.gov)
  • Wild-type cell lines are sold with knockout cell lines only - not available for individual purchase. (abcam.com)
  • EdiGene has the industry's largest off-the shelf diploid library of over 2,800 single clonal knockout (KO) cell lines, targeting over 2,600 genes in commonly used human cancer cell lines such as HeLa, HEK293-T, A549, HCT116, Hep G2 and MCF7. (pharmatimes.com)
  • To support your research on the role and mechanism of SUMO modifications, Horizon has developed a collection of human knockout HAP1 cell lines that lack components of the SUMOylation pathway. (horizondiscovery.com)
  • Choose from over 300 knock-in and knockout cell line models in many standard cancer cell lines such as DLD1, MCF10A, and HCT116. (horizondiscovery.com)
  • RNA Sanger sequencing analysis was performed in AEG‑1‑deficient HT22 cells and wild‑type HT22 cells without knockout (KO). (spandidos-publications.com)
  • EBV infectability and permanent conversion into EBV positive sub-lines is possible by in vitro infection. (sigmaaldrich.com)
  • Researchers from South Korea and Switzerland have developed magnetically actuated microrobots and used them for precise stem cell delivery and transplantation in vitro , ex vivo and in vivo . (physicsworld.com)
  • In our current project we are working on the generation of immortal immune cell lines in order to improve our In vitro Immunization approach. (uni-potsdam.de)
  • Thereby the in vitro Immunization represents an alternative to the conventional hybridoma technique because cell lines would provide permanent and limitless numbers of the immune cells needed and could be generated from different host species. (uni-potsdam.de)
  • First steps in this direction are the characterization of relevant immune cells, optimization of culture conditions and in vitro differentiation from precursor to mature immune cells. (uni-potsdam.de)
  • Objective: Serum is usually added to growth media when mammalian cells are cultured in vitro to supply the cells with growth factors, hormones, nutrients and trace elements. (lu.se)
  • In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)
  • I have a small query on NCI60 cell line expression with specific target gene. (google.com)
  • Human BeWo cell line has been used to study the effects of Δ-9-tetrahydrocannabinol (Δ9-THC) on the proliferation and gene transcription in the BeWo trophoblast cell line. (sigmaaldrich.com)
  • S tem cell research that uses pluripotent stem cells derived from human skin or blood cells has led to numerous discoveries, aided drug development , and proven useful in gene therapies. (the-scientist.com)
  • The cell line market is in the early stages of development as a research tool, where the provision of more choice, with the right gene targets in the right cell lines, will allow the market to rapidly expand. (pharmatimes.com)
  • Except for the B3B3 line that has undergone unproductive gene rearrangement, all other lines express IgH and L mRNAs but do not synthesize any Ig protein (Robert et al. (rochester.edu)
  • In CAR T cell therapy, doctors remove a patient's T cells, implant a gene in them and infuse them back into the patient. (lymphomainfo.net)
  • A stable AEG‑1‑deficient HT22 neuronal cell line was constructed using CRISPR/Cas9 gene‑editing technology. (spandidos-publications.com)
  • Astrocyte elevated gene-1 (AEG-1), which is also known as metadherin and lysine-rich carcinoembryonic antigen-related cell adhesion molecule 1 co-isolated, was first observed to be upregulated in brain astrocytes from patients with HIV-induced Alzheimer's disease ( 1 ). (spandidos-publications.com)
  • Materials and Methods: We have adapted the Burkitt's lymphoma line Ramos to a serum-free medium that supports long-term survival and studied gene expression changes that occurred during the adaptation process. (lu.se)
  • Mutations in the AMN gene prevent cubilin from attaching to the cells in the small intestine and kidneys. (medlineplus.gov)
  • Other CUBN gene mutations affect cubilin's function only in the small intestine, impairing uptake of vitamin B12 into the intestinal cells. (medlineplus.gov)
  • which means both copies of the gene in each cell have mutations. (medlineplus.gov)
  • In this context, in the undifferentiated state, the entropy would be large since fewer constraints exist on the gene expression programmes of the cell. (lu.se)
  • The programmes governing the function and fate of cells are to a large extent driven by the coordinated activity of transcription factors forming complex and dynamic gene regulatory networks (GRNs). (lu.se)
  • The activities of transcription factors and other genes involved in cell fate decisions can be measured by a number of different gene expression quantification experiments. (lu.se)
  • Until recently, and due to technical limitations, for a given cell type such experiments had to be done on an ensemble of many cells and, hence, gene expression quantifi- cations represented the average over a given population. (lu.se)
  • RAMOS Cell Line human has been used to carry out a MTT (colorimetric assay) cell viability assay to measure calcium ionophore-induced cell death in Ramos cells under different conditions and to determine the expression of ZFP36 ring finger protein like 1 (ZFP36L1) in Ramos B cells during different stages of B cell differentiation. (sigmaaldrich.com)
  • Cellular differentiation of this stem cell type occurs when hormonal precursors are added to the medium. (sigmaaldrich.com)
  • Fabricated by 3D laser lithography, the microrobots' porous structure facilitates the attachment, proliferation and differentiation of various stem cells. (physicsworld.com)
  • Fluorescent microscopy images of neural stem cells and their differentiation into astrocytes, oligodendrocytes and neurons on a lab dish, a helical microrobot and a spherical microrobot. (physicsworld.com)
  • The proliferation and differentiation of pluripotent stem cells give rise to progeny that can populate the entire immunologic and hematopoietic systems through committed progenitors of both the lymphoid and myeloid lineages. (medscape.com)
  • M, Peterson C. 2018 Haematopoietic stem suggests that during differentiation a stem cell approaches a stable equili- cells: entropic landscapes of differentiation. (lu.se)
  • Single cells in these populations exhibit different com- stem cell differentiation, Shannon information binations of regulator activity that suggest the presence of multiple theory, entropy configurations of a potential differentiation network as a result of multiple entry points into the committed state. (lu.se)
  • A cell line can harbor thousands of mutations and still be usable for research as long as those mutations are concentrated in irrelevant noncoding areas or don't hit important genes, Nik-Zainal says. (the-scientist.com)
  • Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
  • Using both fetal and adult human skin cells, the researchers introduced the four genes previously reported sufficient for cell reprogramming and compared the efficiency of reprogramming in the presence or absence of large T antigen. (hopkinsmedicine.org)
  • Treatment with honey stops the tumour cells cycle by regulating some genes (cyclin D1, CDK2, p27Kip. (agenciasinc.es)
  • it inhibits cell migration, reduces the ability to form colonies and induces apoptosis or programmed cell death through the modulation of other key genes (p53, caspase-3, c-PARP) and various apoptotic factors," says Maurizio Battino, coordinator of the research. (agenciasinc.es)
  • Ribosomes are factories inside cells where messages coming from genes are decoded and new proteins pieced together on an assembly line. (analytica-world.com)
  • Most changes were subtle, but frequently the genes with altered expression were involved in basal cellular functions such as cell division, cell cycle regulation, apoptosis and cell signalling. (lu.se)
  • Some alterations were restored when the cells were transferred back to serum-containing medium, indicating that expression of these genes was controlled by components in serum. (lu.se)
  • The precise pathophysiology of inherited single cell and multilineage cytopenias has not been elucidated despite the identification of many of the genes mutated in these disorders. (medscape.com)
  • SaneVax: Most medical consumers, and some physicians, are not aware that cell lines used in vaccine production sometimes come from aborted fetal tissues. (sanevax.org)
  • Even when considering distinct cancer subtypes and targeted therapies, most drugs are evenly effective/ineffective throughout all cell lines. (nih.gov)
  • The U.S. Food and Drug Administration (FDA) approved two CAR T-cell therapies in 2017, Kymriah developed by Novartis for the treatment of children with acute lymphoblastic leukemia and Yescarta developed by Kite for adults with advanced lymphomas. (nature.com)
  • Publishing online in Stem Cells on May 29, the team describes a faster and more efficient method of reprogramming cells that might speed the development of stem cell therapies. (hopkinsmedicine.org)
  • It also suppresses the receptor of the epidermal growth factor called EGFR/HER2 and its signalling pathways, which could be an attractive target in cancer therapies because of their important role in the processes of cell survival and proliferation, as well as in those of apoptosis and metastasis. (agenciasinc.es)
  • Evidence suggests that up to a third of established tumor cell lines being used for research are affected by inter- or intra-species cross-contamination or have been wrongly identified. (labmanager.com)
  • X. laevis LG15 cloned adult injected sc with 10,000 15/0 tumor cells. (rochester.edu)
  • X. laevis is the only amphibian where true lymphoid tumors have been discovered and cell lines have been obtained opening new avenues for tumor biology and tumor immunity. (rochester.edu)
  • Some of these tumor lines (15/50, 15/40 and ff-2) remain tumorigenic when transplanted in MHC compatible hosts. (rochester.edu)
  • The 15/0 tumor line derived from the LG-15 clone (MHC a/c haplotype), grows well in LG-15 (Fig. 3) and other LG sharing the same MHC (LG6 and 7). (rochester.edu)
  • In contrast the ff-2 tumor line grows well in F larvae but is rejected by adults. (rochester.edu)
  • In AST, AEG-1 causes glutamatergic excitotoxicity by downregulating the activity of the excitatory amino acid transporter 2 (EAAT2) promoter, leading to neuronal cell death in glioma-induced neurodegenerative disease ( 10 ). (spandidos-publications.com)
  • Finally, the team demonstrated in vivo stem cell transportation using the microrobots in a live nude mouse. (physicsworld.com)
  • Only approximately 5% of compounds showed differential toxicity responses in the cell lines with no obvious correlation to the known in vivo organ toxicity. (nih.gov)
  • The use of such cell lines allows the ex vivo induction of an immune response and makes it independent from experimental animals. (uni-potsdam.de)
  • None of these effects was observed when honey was applied to healthy cell lines," the authors point out, noting the anticarcinogenic potential of this natural product, but insisting on the need for new studies with colon cancer in vivo models to confirm its chemopreventive effects. (agenciasinc.es)
  • Not only can the cell line measure the hormone's potency, but it can also be used to detect neutralizing antibodies directed against HGH," Novik points out. (genengnews.com)
  • DOI=10.1073/pnas.81.2.568 Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.Proc. (mskcc.org)
  • DOI=10.1073/pnas.79.18.5641 Cairncross J.G., Mattes M.J., Beresford H.R., Albino A.P., Houghton A.N., Lloyd K.O., Old L.J. Cell surface antigens of human astrocytoma defined by mouse monoclonal antibodies: identification of astrocytoma subsets. (mskcc.org)
  • Cheri Walker, SVP of corporate development at Abcam, said that the company is "excited to be able to bring the same level of industry-leading quality and data to the cell lines market as we have to research antibodies. (pharmatimes.com)
  • Stem cells derived from adult somatic cells "will carry the mutational history of their past, as well as of any new mutations that occur when you are reprogramming them or growing them in culture," Nik-Zainal says. (the-scientist.com)
  • Most of the human induced pluripotent stem cells stored at major cell line repositories and used in research harbor thousands of DNA errors, a study finds, highlighting the need for improved quality control measures. (the-scientist.com)
  • In order to deposit a cell line someplace like HipSci, researchers only have to demonstrate that the stem cells don't have any missing or duplicated chromosomes or other largescale genetic errors-analyses that would miss the myriad single-nucleotide mutations identified in the new paper. (the-scientist.com)
  • Precise delivery of stem cells to the desired target is, however, crucial for the success of transplantation. (physicsworld.com)
  • Upon application of a rotating magnetic field, the spherical and helical microrobots respectively exhibited rolling and corkscrew motions that propelled them through 3D spaces - such as a blood vessel isolated from a rat brain and a ventricle within a slice of mouse brain - enabling precise targeting of transplanted stem cells. (physicsworld.com)
  • The researchers examined hippocampal neural stem cells (NSCs) grown on the microrobots. (physicsworld.com)
  • They cultured mesenchymal stem cells derived from the human nose on spherical microrobots for transplantation in the mouse, and showed that an externally applied magnetic field could manipulate the cell-loaded microrobots within the animal's hollow abdominal cavity. (physicsworld.com)
  • One challenge to studying blood diseases like sickle cell anemia is that blood stem cells can't be kept alive for very long in the lab, so researchers need to keep returning to patients for more cells to study," says Cheng. (hopkinsmedicine.org)
  • A) Twenty-four hours after infection, PEDV nucleoprotein in infected cells was detected by immunofluorescence assay using fluorescein isothiocyanate- labeled nucleoprotein-specific monoclonal antibody. (cdc.gov)
  • Cytotoxicity was assessed using the MTT assay (cell viability) and Griess reagent (NO release). (bvsalud.org)
  • However, many of these human induced pluripotent stem cell (hiPSC) lines banked in repositories or developed in labs likely harbor thousands of undetected mutations, casting doubt on how generalizable the findings made with them can be, according to research published yesterday (August 11) in Nature Genetics . (the-scientist.com)
  • We therefore assayed both Ca2+i and plasma membrane integrity in fura-2-loaded, single SK-N-SH neuroblastoma cells, using digitized video microscopy and metabolic inhibition (2.5 mM NaCN, 10 mM 2-deoxyglucose) to model the ATP depletion of hypoxia. (jneurosci.org)
  • Blebbing of the cell membrane was evident within 30 min. (jneurosci.org)
  • Ca2+i did not appreciably increase further until the time of cell death, when the loss of plasma membrane integrity allowed unimpeded influx of extracellular Ca2+. (jneurosci.org)
  • AEG-1 has been shown to localize to the cell membrane, cytoplasm, endoplasmic reticulum, nucleolus and nucleus in cancer cells ( 2 ). (spandidos-publications.com)
  • Amnionless attaches (binds) to cubilin, anchoring cubilin to the cell membrane. (medlineplus.gov)
  • The lysate range is expected to be available through the Abcam website in early Q4 2019, with the expanded cell line offering following throughout 2020. (pharmatimes.com)
  • Here we show substantial agreement and biological consilience between drug sensitivity measurements and their associated genomic predictors from two publicly available large-scale pharmacogenomics resources: The Cancer Cell Line Encyclopedia and the Genomics of Drug Sensitivity in Cancer databases. (nature.com)
  • Systematic identification of genomic markers of drug sensitivity in cancer cells. (nature.com)
  • We assess whether drug sensitivity in cancer cell lines is able to discriminate tissue specificity. (nih.gov)
  • A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes. (nature.com)
  • Select organ toxicity potentially results from compound accumulation in a particular tissue, cell types within organs, metabolism, and off-target effects. (nih.gov)
  • Cell Factory engineers contribute to the transition towards a sustainable society by supplying genetically engineered cell factories for fermentation-based production of proteins or biochemicals. (dtu.dk)
  • These studies demonstrated that a combination of three different osmolytes including sugar, sugar alcohol and amino acids/proteins could stabilize Jurkat cells and mesenchymal stromal cells (MSCs) during freezing. (nature.com)
  • In her own research, Dunham is examining how viruses such as HIV, upon hijacking ribosomes, use special tricks that cause the assembly line to slip, as well as how other antibiotics and toxin proteins interact with parts of the ribosome. (analytica-world.com)
  • All these cell lines express specific T-cell lineage markers (CD8, CD5 and XT-1 pan T-cell), and immature thymocytes CTX marker. (rochester.edu)
  • There is a demand for DMSO-free cryoprotectants that maintain cell viability and function after thaw. (nature.com)
  • immortalized duck intestinal epithelial cell (MK-DIEC) line, which was generated from the intestinal tissues of Mahesh Khatri a 19- day-old white Pekin duck embryo. (cdc.gov)
  • We find that cancer-specific drugs do not show higher efficacies in cell lines representing the respective tissues. (nih.gov)
  • Stem cell therapy is an emerging method for restoring damaged tissues or treating a variety of diseases, from cancer to degenerative neural disorders like Alzheimer's disease. (physicsworld.com)
  • Ultimately, they found that ultraviolet (UV) radiation, possibly linked to sunlight exposure prior to harvesting the cells, was causing significant levels of DNA damage to skin-derived hiPSCs-sometimes inducing tens of thousands of mutations. (the-scientist.com)
  • Those 'normal' cell lines have as many mutations as [some] cancers," Nik-Zainal tells The Scientist . (the-scientist.com)
  • The sequencing revealed that 72 percent of the skin cell-derived hiPSCs in the HipSci bank showed signs of mutations. (the-scientist.com)
  • Further analysis revealed that mutations, especially the BCOR mutations in the blood-derived lines, can occur after reprogramming, meaning they didn't originate from the human donors but rather arose as the cells replicated in the lab, likely through selective pressures the cells experience while growing and dividing in a dish. (the-scientist.com)
  • But "it's not like you're a bad scientist if your cells acquire mutations," says Loring. (the-scientist.com)
  • Researchers need to start with a reliable process for commercial biopharmaceutical production in living cells. (the-scientist.com)
  • Despite the varied causes of irreproducibility from factors that are difficult to control, cell line authentication (CLA) is a relatively simple operation that assures that the cells researchers believe they are working with are actually those cells, and that they are contaminant-free. (labmanager.com)
  • Spanish and Italian researchers have proven that when honey from strawberry trees, a product typical of Mediterranean areas, is added to colon cancer cells grown in the laboratory, cell proliferation is stopped. (agenciasinc.es)
  • The Salk professor developed the institute's first neurobiology lab and used it to develop cell lines, describe amyloid β toxicity, and screen for compounds that protect against neurodegeneration. (the-scientist.com)
  • Our results suggest that from a general screening perspective, different cell lines have relatively equal value in assessing general cytotoxicity and that specific organ toxicity cannot be accurately predicted using such a simple approach. (nih.gov)
  • Synthesis and toxicity evaluation in breast cancer cell lines. (lu.se)
  • A human ovarian small cell carcinoma line (BIN-67) expresses abundant calcitonin (CT) receptors (CTR) (143,000 per cell) that are coupled, to adenylate cyclase. (jci.org)
  • The genetically engineered T cells are then awakened to go after the cancer. (lymphomainfo.net)
  • A meta-analysis (n = 52 randomized trials encompassing 9,387 patients) of the Non-Small-Cell Lung Cancer Collaborative Group indicated that cisplatin- based chemotherapy provides a significant survival benefit for patients with locally advanced or metastatic disease. (cancernetwork.com)
  • Results and Conclusions: The adaptation process was characterized by initial cell population growth arrest, and after that extensive cell death, followed by proliferation and long-term survival of clonal cultures. (lu.se)
  • HEL cell is capable of induced globin synthesis, producing mainly Gγ and Aγ chains. (wikipedia.org)
  • In the present studies we investigated the effects of BMP-9 on ACh synthesis in the cholinergic mouse SN56T17 septal cell line. (rti.org)
  • BMP-9 increased ACh synthesis in these cells up to 2.5-fold in a time- and dose-dependent, saturable manner. (rti.org)
  • Other types of cell lines are also available in X. laevis including: fibroblasts lines of the different strains and LG clones, and a kidney cells line (A6). (rochester.edu)
  • The microenvironment, consisting of lymphocytes, macrophages, fibroblasts, endothelial elements, and stroma in which hematopoietic cells reside, creates a regulatory niche that determines the local area network. (medscape.com)
  • Cloning, characterization, and expression of a human calcitonin receptor from an ovarian carcinoma cell line. (jci.org)
  • Adults in this clinical situation are eligible for a randomized, open-label, phase 3 trial to assess the safety and efficacy of zipalertinib (Taiho) alongside standard first-line platinum-based chemotherapy. (medscape.com)
  • For chemotherapy, two-drug cytotoxic regimens that include a platinum agent are generally preferred for first-line therapy. (medscape.com)
  • Abcam has entered the cell editing market through acquisition of the entire live cell line and lysates portfolio of EdiGene Inc, a company focused on developing genome editing technologies into novel therapeutics for a broad range of diseases and into creative solutions to advance drug discovery. (pharmatimes.com)
  • Add 2 ml of the Jurkat cell suspension to a new sterile T75 flask containing 20 ml of complete growth medium and a final concentration of 1 mg/ml G418. (neb.com)
  • This study examined the post-thaw recovery of Jurkat cells cryopreserved in single osmolyte solutions containing sucrose, glycerol or isoleucine, as well as in a combination of the three osmolytes. (nature.com)
  • Post-thaw recovery of Jurkat cells in single osmolyte was low. (nature.com)
  • The objective of this investigation is to understand in more detail the relationships amongst the osmolytes present in these solutions and Jurkat cell recovery. (nature.com)
  • Amnionless helps transfer the cubilin-vitamin B12 complex into the intestinal cell. (medlineplus.gov)
  • In addition, further engineered cell lines can be offered, as reporter cell lines, or overexpression stable cell lines. (biocat.com)
  • Flp-In™ 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In™ expression vector. (thermofisher.com)
  • Add 10 ml of fresh complete growth medium and gently resuspend cell pellet by pipetting up and down 2-3 times. (neb.com)
  • 3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. (abcam.com)
  • The results, published in the Journal of Functional Foods, show that strawberry tree honey is capable of inhibiting the proliferation of tumour cells cultivated in laboratory plates - more specifically, those of a line of human colon adenocarcinoma (HCT-116) and another with metastatic characteristics (LoVo), widely used to investigate this type of tumour. (agenciasinc.es)
  • Strawberry tree honey is capable of inhibiting the proliferation of tumour cells on a line of human colon adenocarcinoma (HCT-116) and another with metastatic characteristics (LoVo). (agenciasinc.es)
  • Enrolment of older adults with advanced or metastatic non-small cell lung cancer in first-line clinical trials in the multicentre ESME cohort. (bvsalud.org)
  • Inclusion criteria were patients with advanced or metastatic non-small cell lung cancer (AMNSCLC) aged 70 years or older, with at least one line of systemic treatment from 01 January 2015 to 31 December 2018. (bvsalud.org)
  • Second-line and subsequent therapy may involve single agents (eg, taxanes) or molecular therapy, such as ramucirumab to target vascular endothelial growth factor (VEGF) receptor or trastuzumab for metastatic adenocarcinoma that overexpresses HER2. (medscape.com)
  • It has also been used to study placental transfer of highly purified non-dioxin-like polychlorinated biphenyls in BeWo cells. (sigmaaldrich.com)
  • A new partnership opens the door for a source of highly purified human primary cells and defined cell culture media. (the-scientist.com)
  • The HEL cell line is an immortalised cell line from a 30-year old male Acute erythroid leukemia patient, used in biomedical research. (wikipedia.org)
  • According to study coauthor and Cambridge Biomedical Research Campus medical geneticist Serena Nik-Zainal , the study reveals that the level of quality control involved in such stem cell research may not be up to snuff. (the-scientist.com)
  • It's a problem, but it's solvable," says Jeanne Loring , a stem cell researcher at the Scripps Research Institute who didn't work on the study. (the-scientist.com)
  • As it stands now, scientists using these hiPSCs for research or clinical applications only need to screen and characterize their cell lines, whether they're developed in-house or taken from a repository, to whatever extent is required by a journal or the reviewers assessing their work. (the-scientist.com)
  • This cell line may be licensed nonexclusively for research or commercial purposes. (mskcc.org)
  • The single largest bank of isogenic cell lines with over 7,500 cell lines to choose from and trusted by academia, biotech, and pharma research labs. (horizondiscovery.com)
  • Can't find the right cell model for your research? (horizondiscovery.com)
  • The research was funded by the National Institutes of Health and the Johns Hopkins Institute for Cell Engineering. (hopkinsmedicine.org)
  • These pre-existing lines have proven insufficient for most research. (ca.gov)
  • Pera is professor of cell and neurobiology at the Keck School of Medicine and Director of the Eli & Edythe Broad CIRM Center for Regenerative Medicine and Stem Cell Research at the University of Southern California. (ca.gov)
  • These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus. (thermofisher.com)
  • Dimethyl sulfoxide (DMSO) has been the standard cryopreservation agent for freezing cells since the 1960 s 2 . (nature.com)
  • Prolor Biotech obtained a nonexclusive license to The University of Queensland's (UQ) human growth hormone (HGH) receptor cell line. (genengnews.com)
  • At some point, the Seattle biotech company Cell Therapeutics Inc (CTI) should earn an entry in the Guinness Book of World Records for utter and. (lymphomainfo.net)
  • It has beenevaluated in non-small-cell lung cancer (NSCLC) as front-line chemotherapyin a comprehensive phase II evaluation. (cancernetwork.com)
  • It has beenevaluated in nonâ small-cell lung cancer (NSCLC) as front-line chemotherapyin a comprehensive phase II evaluation. (cancernetwork.com)
  • Non-small-cell lung cancer (NSCLC)-the most prevalent histologic form-accounts for 75% to 80% of lung cancers. (cancernetwork.com)
  • Surgery is recommended for patients with stage I or II non-small cell lung cancer NSCLC) and may provide the best possibility for a cure. (medscape.com)
  • Untreated advanced non-small cell lung cancer (NSCLC). (medscape.com)
  • Within this study line, you will obtain competences in designing, constructing, and optimizing cell factories using either bacterial, yeast, molds, plant or animal cell cultures. (dtu.dk)
  • Proliferation and cell cycle progression of the serum-free cultures closely mimicked that of serum-dependent cells. (lu.se)
  • Near confluent cells were infected with been isolated in the United States, making development of a PEDV at a multiplicity of infection of 0.1. (cdc.gov)
  • B) PEDV-induced cytopathic effect in MK-DIEC cells 36 h after infection. (cdc.gov)
  • This practice adds to the complexity of the workflow associated with preservation of cells using DMSO. (nature.com)
  • Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose. (abcam.com)
  • 1 x 10E7 cells are supplied frozen in 1 ml of 90% complete medium and 10% DMSO. (thermofisher.com)
  • For 75 cm 2 flasks, use approximately 10 5 cells/flask and subculture every 3-4 days. (neb.com)
  • Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x10 4 cells/cm 2 . (abcam.com)
  • Remove and discard 10 ml of old growth medium from above the cell pellet. (neb.com)
  • Large cancer cell line collections broadly capture the genomic diversity of human cancers and provide valuable insight into anti-cancer drug response. (nature.com)
  • The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity. (nature.com)
  • Cancer cell lines have a prominent role in the initial stages of drug discovery, facilitating high-throughput screening of potential drugs. (nih.gov)
  • Chimeric antigen receptor (CAR) T-cell therapy is a rapidly growing therapy for the treatment of cancer 1 . (nature.com)
  • SK-MG-09 is a human brain cancer cell line. (mskcc.org)
  • Next, Jeon and colleagues established the feasibility of targeted cell delivery using microrobots loaded with human colon cancer cells in a body-on-a-chip microfluidic cell culture platform that mimics parts of a liver tumour network. (physicsworld.com)
  • MD Anderson Cancer Center is in line to receive $100 million in publicly traded stock as part of a licensing agreement that involves one of the most exciting emergent treatment avenues in oncology. (lymphomainfo.net)
  • it involves customizing T cells to find and kill cancer cells. (lymphomainfo.net)
  • Genetically engineering our patients' immune-system T cells to efficiently attack and destroy cancer cells represents one of the most exciting approaches with curative potential in oncology today," said MD Anderson President Dr. Ron DePinho. (lymphomainfo.net)
  • We believe coupling MD Anderson's unique CAR T cell approach with the powerful technologies of ZIOPHARM and Intrexon will allow us to build T cells that hit cancer harder, with greater precision, under tighter control and with potentially fewer side effects for patients. (lymphomainfo.net)
  • Merkel Cell Carcinoma (MCC) is a very rare and aggressive skin cancer that usually develops when a person is in his or her 70s. (lymphomainfo.net)
  • The cytotoxicity and anti-tumour effects of strawberry tree honey in relation to colon cancer lines increased with the amount and time of treatment. (agenciasinc.es)
  • Pluristem Therapeutics , a leading developer of placenta-based cell therapy products, has announced that it has completed development of its second major product line, called PLX-RAD. (israel21c.org)
  • We believe that our state-of-the-art technology platform can be used to create additional cell products from the placenta, tailored to potentially deliver targeted treatments for a variety of new indications," stated Zami Aberman, Chairman and CEO of Pluristem. (israel21c.org)
  • The first human, trophoblastic endocrine cell type to be maintained in continuous culture. (sigmaaldrich.com)
  • Discover the ins and outs of the cell culture workflow. (the-scientist.com)
  • 2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. (abcam.com)
  • From "Cell lines from aborted human fetus in vaccine production," by Ragnhild Madsen, vaccine questions. (sanevax.org)
  • But Anglican Archbishop of Sydney Glenn Davies said there were ethical questions about the potential vaccine, because scientists have used cell lines from an electively aborted foetus. (abc.net.au)
  • There are strong ethical regulations surrounding the use of any human cell, particularly foetal human cells,' Dr Coatsworth said. (abc.net.au)
  • Here, we investigated the utility of hepatic-, cardiac-, and kidney-derived cell lines to (1) accurately predict cytotoxicity and (2) to accurately predict specific organ toxicities. (nih.gov)
  • The response pattern varied depending on time and type of cell line used for analysis, although the results indicate a higher cytotoxicity for EPH in short-term tests. (bvsalud.org)