Cell kinetics. Medical search. Frequent questions

While substantial progress has been made to delineate the developmental pathway of T-cell lineage commitment, still little is known about the relationship between differentiation competence and the remarkable expansion of the earliest (DN1 stage) T-cell progenitors. (lu.se)
To satisfy differentiation kinetics and overall cell yield data, our models require that adult DN1 cells divide multiple times before becoming competent to progress into DN2 stage. (lu.se)
Single-Cell Transcriptomics Reveals that Differentiation and Spatial Signatures Shape Epidermal and Hair Follicle Heterogeneity. (crossref.org)
In recent years, interest in the possible molecular regulators of cell proliferation and differentiation in a wide range of regeneration models has grown significantly, but the cell kinetics of this process remain largely a mystery. (mdpi.com)
Mosaic analyses reveal that Id2a function in facilitating both cell cycle progression and neuronal differentiation in the retina is non-cell-autonomous, suggesting that Id2a functions upstream of the extrinsic pathways that regulate retinogenesis. (biologists.com)
Comparative analysis of dental pulp stem cells and stem cells from human exfoliated teeth in terms of growth kinetics, immunophenotype, self-renewal and multi lineage differentiation potential for future perspective of calcified tissue regeneration. (bvsalud.org)
The isolated cells were characterized for the presence of stem cell markers by immunophenotyping and immunofluorescence staining , and tri-lineage differentiation. (bvsalud.org)
Its prominent properties are A lack of cell differentiation Local invasion of adjoining tissue Metastasis, which is spread to distant sites through. (msdmanuals.com)
Objective: To characterize pulp stem cells and evaluate their capacity for expansion and differentiation in vitro. (bvsalud.org)
Results: It was observed that the pulp stem cells exhibited the capacity to adhere to plastic and a high rate of expansion and, after detection with specific stains, it was shown that the cells were capable of differentiation into osteoblasts and chondroblasts, but not into adipocytes. (bvsalud.org)
Stem cells are cells with a low degree of differentiation that have the capacity to reproduce and can generate differentiated cells of several different types of tissues 1 . (bvsalud.org)
The objective of this study is to isolate and characterize stem cells from the pulp of permanent third molars and analyze the cells obtained in terms of their morphology and chondrogenic, osteogenic and adipogenic cell differentiation. (bvsalud.org)

In this thesis, total internal fluorescence microscopy (TIRF) and single-molecule imaging were used to observe and characterize protein-functionalized supported lipid bilayers (SLBs) interacting with immune cells to obtain the binding kinetics of various protein-protein pairs. (lu.se)
For this reason, the influence of an adhesion molecule, rat CD2, on the obtained binding kinetics of the human CD4 co-receptor was initially examined showing that the accumulation of CD4 was influenced when having a high concentration of bound CD2 inside the cell-SLB contacts. (lu.se)
Binding Kinetics and Lateral Mobility of HSV-1 on End-Grafted Sulfated Glycosaminoglycans. (lu.se)
Cell Membrane Derived Platform To Study Virus Binding Kinetics and Diffusion with Single Particle Sensitivity. (lu.se)

Using biophysical techniques to measure water flux through the plasma membrane of wild-type (WT) and AQP4 knockout (KO) astrocytes and of an astrocyte cell line (DI TNC1) transfected with AQP4 or AQP1, we here show that AQP-mediated fast swelling kinetics play a key role in triggering and accelerating RVD. (elsevierpure.com)
Using calcium imaging, we show that AQP-mediated fast swelling kinetics also significantly increases the amplitude of calcium transients inhibited by Gadolinium and Ruthenium Red, two inhibitors of the transient receptor potential vanilloid 4 (TRPV4) channels, and prevented by removing extracellular calcium. (elsevierpure.com)

In humans, muscle satellite cell (SC) enumeration is an important measurement used to determine the myogenic response to various stimuli. (mcmaster.ca)
Although IHC is a powerful tool for SC analysis, FC is a fast, reliable and effective method for SC quantification as well as a more informative method for cell cycle kinetics of the SC population in humans. (mcmaster.ca)

Using loss-of-function and overexpression assays in zebrafish, we demonstrate that Id2a levels modulate retinoblast cell cycle kinetics and thereby influence neuron and glia formation in the retina. (biologists.com)

In biochemistry, Michaelis-Menten kinetics, named after Leonor Michaelis and Maud Menten, is the simplest case of enzyme kinetics, applied to enzyme-catalysed reactions of one substrate and one product. (wikipedia.org)
Enzyme kinetics is the study of the rates of enzyme-catalysed chemical reactions . (wikipedia.org)

J Cell Biol (1987) 105 (6): 2713-2721. (rupress.org)
1983. J. Cell Biol. (rupress.org)
1984. J. Cell Biol. (rupress.org)
J Cell Biol (1988) 106 (3): 677-685. (silverchair.com)

In this context, we employ single particle tracking together with atomic-force-microscopy-based force spectroscopy to study how virus attachment, detachment and diffusion is modulated at the cell surface. (lu.se)
Resulting PBPK model simulations accurately predicted in vivo concentrations of TCPy in rat saliva, suggesting that diffusion coefficients of unbound TCPy are consistent between cell culture systems and in vivo. (cdc.gov)
Virus entry is a complex dynamic multistep process requiring a series of fine-tuned events mediating virus diffusion through the glycocalyx, its attachment to the cell membrane and lateral diffusion to the point of entry. (lu.se)
We further, take advantage of total internal fluorescence microscopy and single particle tracking to study, on a single particle level, virus attachment to and diffusion on the cell surface. (lu.se)

mitotically active cells of intact segments do not significantly contribute to the blastemal cellular sources. (mdpi.com)
In addition to direct viral sensing, cells have also evolved ways to detect the indirect effects of virus infection, such as nuclear or mitochondrial damage caused by the heavy cellular burden imposed by virus replication. (nature.com)
Hoskins, MH & Dong, C 2006, ' Kinetics analysis of binding between melanoma cells and neutrophils ', MCB Molecular and Cellular Biomechanics , vol. 3, no. 2, pp. 79-87. (psu.edu)
Kinetic descriptions of protein binding were added to the cellular computational model and parameterized with data of TCPy protein binding in cell culture medium. (cdc.gov)
The cellular kinetics of particular cancers is an important consideration in the design of antineoplastic drug regimens and may influence the dosing schedules and timing intervals of treatment. (msdmanuals.com)
Analysis of the cellular phenotype showed that the cells were negative for CD45, CD69, CD117 and HLADR, and positive for CD13, CD44, CD73, CD90 and CD105. (bvsalud.org)

These CAR-T cells are characterized by highly activated T cell signaling and significant tumor infiltration. (nature.com)
The most potent nanobody-based CAR-T cells show inhibition of large tumor xenografts in mice with rigorous T-cell signaling and significant T-cell infiltration into the tumor. (nature.com)
Internalization kinetics of 68 Ga- and 177 Lu-PSMA I&T were investigated using the same cell line, and biodistribution studies were performed in LNCaP tumor-bearing CD-1 nu/nu mice. (snmjournals.org)
Uptake of 68 Ga-/ 177 Lu-PSMA I&T in LNCaP tumor cells is highly efficient and PSMA-specific, as demonstrated by competition studies both in vitro and in vivo. (snmjournals.org)
Tumor targeting and tracer kinetics in vivo were fast, with the highest uptake in tumor xenografts and kidneys (both PSMA-specific). (snmjournals.org)
Tumor volumes prior to initial therapy for 33 patients with small cell carcinoma of the lung were determined from serially measurable roentgenographic lesions. (johnshopkins.edu)
Initial exponential tumor growth is followed by a plateau phase when cell death nearly equals the rate of formation of daughter cells. (msdmanuals.com)

The properties of the mutant cells suggest that proper functioning of pH regulatory mechanisms in early endocytic compartments is critical for many pH-mediated processes of endocytosis. (rupress.org)
Although these mechanisms are often a complex series of steps, there is typically one rate-determining step that determines the overall kinetics. (wikipedia.org)
Ribozymes also perform a more limited set of reactions, although their reaction mechanisms and kinetics can be analysed and classified by the same methods. (wikipedia.org)
Mechanisms in CD4 antibody-mediated transplantation tolerance: kinetics of induction, antigen dependency and role of regulatory T cells. (ox.ac.uk)
Kinetics and mechanisms of the gas-phase reactions of the hydroxylradical with organic compounds under atmospheric conditions. (cdc.gov)
2002), nothing is known about long-term there is concern about translocation of ultrafine particles (UFP) translocation and whether clearance kinetics of ultrafine parti- from the lungs into systemic circulation and uptake in transpul- cles differ from that of larger particles, as one may expect from differing clearance mechanisms for micrometer-sized particles versus UFP. (cdc.gov)
The aim of our research is elucidate the mechanisms by which viral pathogens interact with the cell'membrane to crosses it and penetrate into the cell. (lu.se)

Rational design of chimeric antigen receptor T (CAR-T) cells based on the recognition of antigenic epitopes capable of evoking the most potent CAR activation is an important objective in optimizing immune therapy. (nature.com)
The results showed a relatively small spread in the rCD2-rCD48T92A binding affinity values despite the considerable spread of receptor densities within the cell population. (lu.se)
Even though the CD4 co-receptor increases the sensitivity of T cell signalling manyfold, it binds to its ligand, peptide major histocompatibility complex II (pMHCII), with the lowest binding affinity known to this day. (lu.se)
To date it remains unclear how exactly the hair cell receptor potential is converted into an afferent firing pattern at this unusual synapse. (jneurosci.org)
The T cell receptor (TCR)-peptide-MHC (pMHC) interaction is the only antigen-specific interaction during T lymphocyte activation. (ox.ac.uk)
Kinetics of early T cell receptor signaling regulate the pathway of lytic granule delivery to the secretory domain. (ox.ac.uk)

As a result, systemic/widespread chemotherapy is usually injected into the bloodstream to eliminate the remaining cancer cells. (imechanica.org)

Cell-free protein synthesis (CFPS) reactions have grown in popularity with particular interest in applications such as gene construct prototyping, biosensor technologies and the production of proteins with novel chemistry. (ncl.ac.uk)
We show that a novel reaction buffer outperforms the reference reaction by 400 % and importantly reduces failures in CFPS across batches of cell lysates, strains of E. coli, and in the synthesis of different proteins. (ncl.ac.uk)
Despite the fact that the molecular aspects responsible for amyloid pathologies have not been deciphered, interactions of misfolded proteins with cell membranes appear to play essential roles in these disorders. (jnkinhibitor.com)
Analysis of protein levels of desmoplakins I and II by immunoprecipitation with an antiserum that reacts specifically with an epitope common to both proteins revealed that desmoplakins I and II are synthesized and accumulate at steady state in a ratio of 3-4:1 (in the absence or presence of cell-cell contact). (silverchair.com)
The kinetics of desmoplakins I and II stabilization and assembly were analyzed after partitioning of newly synthesized proteins into a soluble and insoluble protein fraction by extraction of whole cells in a Triton X-100 high salt buffer. (silverchair.com)

It was concluded that the small number of constitutively expressed active β 2 -integrins on PMNs are sufficient to bind to ICAM-1 expressed on melanoma cells and that the intrinsic dissociation rate for these adhesion molecules appear to be more dependent on what method is used to determine them than on what cells express them. (psu.edu)
We used a laminar flow chamber to measure, first, 2D TCR-pMHC dissociation kinetics of peptides of various activating potency in a cell-free system in the force range (6 to 15 pN) previously associated with catch-slip transitions and, second, 2D TCR-pMHC association kinetics, for which the method is well suited. (ox.ac.uk)

Whole-cell patch-clamp recordings from calyx endings were performed in an in vitro whole-tissue preparation of the rat vestibular crista, the sensory organ of the semicircular canals that sense head rotation. (jneurosci.org)
Suppressor cell induction in vitro. (ox.ac.uk)
The induction of antigen-specific suppressor cells in vitro, using high concentrations (100 mug/ml) of keyhole limpet hemocyanin (KLH) in Marbrook flasks is described. (ox.ac.uk)
Pulp stem cells (PSCs) are able to form ectopic dentin in vitro and in vivo and also to generate a dentine-pulp complex composed of a mineralized matrix, with dentinal tubules that are aligned and filled with odontoblastic prolongations, containing vascularized pulp tissue, in a similar arrangement to that observed in natural dental structures 3,6 . (bvsalud.org)
Our research strategy widely relies of the use of artificial lipid bilayers to mimic in vitro the basic molecular architecture of the cell membrane. (lu.se)

However, proliferative indices alone do not suffice to characterise cell growth. (bmj.com)
A growth curve was plotted to determine population doubling time , while colony forming numbers and efficiency was determined at plating cell densities of 5.6, 11.1 and 22.2 / cm2. (bvsalud.org)
Experiments suggest that the abilities to invade, migrate, and successfully implant and stimulate new blood vessel growth are all important properties of the cells that cause metastases, which are likely a subpopulation of the primary cancer. (msdmanuals.com)

Change of interferon-γ-producing T-cell responses to severe acute respiratory syndrome coronavirus 2-specific overlapping peptides in paired samples classified with symptom severities. (ajtmh.org)
Copper(ii) complexes with short peptides based on the second cell binding site of fibronectin, PHSFN and PHSEN, have been characterized by potentiometric, UV-vis, CD, EPR and NMR spectroscopic methods. (cnr.it)

In the first part of this thesis, the interaction between the rat CD2 (rCD2) adhesion protein and its ligand rat CD48T92A (rCD48T92A), a high-affinity mutant of the wild type rat CD48, was used to establish a new method of obtaining single-cell binding affinities of T cells interacting with SLBs using imidazole titrations. (lu.se)

In my presentation, I will show how model membranes of varying complexity can be used in complement to virological investigations to study how viruses interact at the cell surface and how such interactions are optimized, on a molecular level, to promote efficient viral entry and infection. (lu.se)
The interactions between melanoma cells and PMNs are mediated by the β 2 -integrins expressed by PMNs and intercellular adhesion molecules (ICAM-1) expressed on melanoma cells. (psu.edu)
In this study, the kinetics of these interactions was studied using a parallel plate flow chamber. (psu.edu)

Like all plus-strand RNA viruses, the SARS-CoV-2 replication process within cells requires de novo production of viral RNA species, including single-strand (ss)RNA and double-strand (ds)RNA that can be sensed by cytosolic pattern recognition receptors (PRRs) subsequently activating antiviral pathways 7 . (nature.com)
This is the first pilot-scale mammalian cell-based viral rabies vaccine production study using microcarrier technology reported in Turkey. (researchsquare.com)
Today, Vero cells are considered a more suitable substrate to produce viral vaccines. (researchsquare.com)
Furthermore, the complete viral replication kinetics of all episodes (including both the ascending and the descending phases of the active infection) was examined in each patient. (unimib.it)

Robbins and co-workers have obtained Chinese hamster ovary (CHO) cell mutants that are pleiotropically defective in endocytosis and deficient in ATP-dependent acidification of endosomes isolated by density centrifugation (Robbins, A. R., S. S. Peng, and J. L. Marshall. (rupress.org)
Our results showed differentially delayed assembly rates of respiratory chain complexes I, III and IV amongst mutants belonging to different mtDNA haplogroups, revealing that specific mtDNA polymorphisms may modify the pathogenic potential of LHON mutations by affecting the overall assembly kinetics of OXPHOS complexes. (nih.gov)
The investigators found that the resultant transfected cells showed defects in ATP2A2 protein expression (15 mutants), ATP hydrolysis (29 mutants), calcium transport (4 mutants), and calcium binding and kinetics (3 mutants). (medscape.com)

Studying an enzyme's kinetics in this way can reveal the catalytic mechanism of this enzyme, its role in metabolism , how its activity is controlled, and how a drug or a modifier ( inhibitor or activator ) might affect the rate. (wikipedia.org)

When the value of the Michaelis constant K m {\displaystyle K_{\mathrm {m} }} is numerically equal to the substrate concentration, the reaction rate is half of V {\displaystyle V} . Biochemical reactions involving a single substrate are often assumed to follow Michaelis-Menten kinetics, without regard to the model's underlying assumptions. (wikipedia.org)

Conclusions: The cells isolated from dental pulp exhibited characteristics compatible with those expected for mesenchymal stem cells (MSCs) and are good candidates for cell therapy applications and tissue bioengineering. (bvsalud.org)

59. Shin YH, Lee SJ, Jung J. Extracellular ATP inhibits Schwann cell dedifferentiation and proliferation in an ex vivo model of Wallerian degeneration. (jnkinhibitor.com)

Human soluble (S) and membrane-bound (MB) catechol O-methyltransferase (COMT, EC 2.1.1.6) enzymes have been expressed at sufficiently high levels in Escherichia coil and in baculovirus-infected insect cells to allow kinetic characterization of the enzyme forms. (lu.se)
In the absence of cell-cell contact, both the soluble and insoluble pools of desmoplakins I and II are unstable and are degraded rapidly (t1/2 approximately 8 h). (silverchair.com)
Upon induction of cell-cell contact, the capacity of the insoluble pool increases approximately three-fold as a proportion of the soluble pool of newly synthesized desmoplakins I and II is titrated into the insoluble pool. (silverchair.com)

Amputation-induced proliferation occurred predominantly within the epidermal and intestinal epithelium, as well as wound-adjacent muscle fibers, where clusters of cells at the same stage of the cell cycle were found. (mdpi.com)
Id2a-dependent defects in retinoblast proliferation lead to microphthalmia and to an absence of nearly all differentiated inner and outer nuclear layer cell types. (biologists.com)
Owing to high proliferation rate, multipotency and self -renewal capability, dental pulp stem cells (DPSC) and stem cells from human exfoliated teeth (SHED) have become stem cell source of choice for cell based regenerative therapies . (bvsalud.org)
Many factors are involved in causing and permitting the unregulated proliferation of cells that occurs in cancer. (msdmanuals.com)
Overview of Cancer Cancer is an unregulated proliferation of cells. (msdmanuals.com)

On prostate cancer (PC) cells, the cell surface enzyme prostate-specific membrane antigen (PSMA), also known as glutamate carboxypeptidase II or N -acetyl- l -aspartyl- l -glutamate peptidase, is highly upregulated, whereas it shows low or no expression in the normal prostate ( 3 ). (snmjournals.org)
This was investigated by a series of adoptive transfer experiments in which mAb-treated cells were removed from therapeutic antibody at defined times after skin grafting, and exposed to fresh antigen in the absence of further mAb treatment. (ox.ac.uk)
If antibody therapy is continued for the full 5 weeks, T cell tolerance can still be established, suggesting that antibody therapy does not prevent lymphocytes from registering the presence of antigen. (ox.ac.uk)
In contrast, "parked" cells exposed to fresh antigen at any time up to 4 months remained tolerant and "resistant" indefinitely. (ox.ac.uk)
I. Kinetics of induction of antigen-specific suppressor cells. (ox.ac.uk)

Our results show that SARS-CoV-2 directs a cGAS-STING mediated, NF-κB-driven inflammatory immune response in human epithelial cells that likely contributes to inflammatory responses seen in patients and could be therapeutically targeted to suppress severe disease symptoms. (nature.com)
For SARS-CoV-2, lung epithelial cells are the primary site of infection and therefore are responsible for initiating immune responses to virus infection. (nature.com)
Kinetics of desmosome assembly in Madin-Darby canine kidney epithelial cells: temporal and spatial regulation of desmoplakin organization and stabilization upon cell-cell contact. (silverchair.com)
To address this problem, we have initiated a study of the regulation of assembly of one component of the junctional complex, the desmosome, during induction of cell-cell contact in cultures of Madin-Darby canine kidney epithelial cells. (silverchair.com)

Upon induction, both cell types differentiated into bone , fat and cartilage like cells . (bvsalud.org)

Building on these observations, we found a specific activation of NF-κB and a block of IRF3 nuclear translocation in SARS-CoV-2 infected cells. (nature.com)

Moreover, cGAS is an important molecule for regulating basal expression levels of cell-intrinsic immune genes in cells and is, therefore, a central protein in immune responses to virus infection 10 . (nature.com)
significantly, the stabilization of this population of newly synthesized desmoplakins I and II is blocked by the addition of cycloheximide at the time of cell-cell contact, indicating that the coordinate synthesis of another protein(s) is required for protein stabilization. (silverchair.com)
TCPy protein binding in cell culture medium and plasma were measured using ultracentrifugation and gas chromatography-mass spectrometry. (cdc.gov)
In a different study, in which researchers systematically analyzed mutations identical to those found in patients with Darier disease, mutant SERCA2 protein aggregates were found to cause stress to the endoplasmic reticulum, subsequently inducing cell apoptosis. (medscape.com)

In enzyme kinetics, the reaction rate is measured and the effects of varying the conditions of the reaction are investigated. (wikipedia.org)

Similar to the results with RSV-IAV coinfection, this analysis showed that the increased disease severity observed during murine IAV-RV or IAV-CoV2 coinfection was likely due to slower clearance of IAV infected cells by the other viruses. (biorxiv.org)
Although several comparative studies have been performed correlating CMV and EBV DNA loads in whole blood (WB) versus plasma, to our knowledge, no studies to date have analyzed the kinetics of both viruses in the 2 blood compartments. (unimib.it)

Detailed and quantitative understanding of how reaction components affect kinetic responses and robustness is imperative for future deployment of cell-free technologies. (ncl.ac.uk)

The suppressor cells were T cells, as judged by their sensitivity to anti-Thy-1.2, heterologous anti-T, but not anti-B antisera. (ox.ac.uk)
Some drugs work only during a specific phase of the cell cycle, requiring prolonged administration to catch dividing cells during the phase of maximal sensitivity. (msdmanuals.com)

When enzymes bind multiple substrates, such as dihydrofolate reductase (shown right), enzyme kinetics can also show the sequence in which these substrates bind and the sequence in which products are released. (wikipedia.org)

In the present study, we demonstrate that B7-H3 CAR-T cells targeting the IgC domain are more active than those targeting the IgV domain in pancreatic ductal adenocarcinoma (PDAC) and neuroblastoma (NB) mouse preclinical models. (nature.com)
In this study, FC analysis revealed that the number of Pax7(+) cells per milligram isolated from 50 mg of fresh tissue increased 36% 24 h after exercise-induced muscle injury (300 unilateral maximal eccentric contractions). (mcmaster.ca)
In this retrospective noninterventional multicenter cohort study, the kinetics of CMV and EBV DNA in 121 hematopoietic stem cell transplantation (HSCT) recipients were investigated by analyzing in parallel 569 and 351 paired samples from 80 and 58 sequential episodes of CMV and EBV DNAemia, respectively. (unimib.it)
All together our study provides evidence that (1) AQP influenced swelling kinetics is the main trigger for RVD and in mediating calcium signaling after hypotonic stimulus together with TRPV4, and (2) calcium influx from the extracellular space and/or TRPV4 are not essential for RVD to occur in astrocytes. (elsevierpure.com)
Taken together, these examples illustrate the potential of artificial cell membrane mimics in the study of processes occurring at the surface of a cell and demonstrate how such biophysical data can complement more classical cell-biology experiments. (lu.se)

Here, we generate dromedary camel nanobodies targeting B7-H3 and demonstrate that CAR-T cells, based on the nanobodies recognizing the IgC but not IgV domain, had potent antitumour activity against large tumors in female mice. (nature.com)
Single-cell transcriptome RNA sequencing coupled with functional T-cell proteomics analysis uncovers the top-upregulated genes that might be critical for the persistence of polyfunctional CAR-T cells in mice. (nature.com)
When tolerant cells were "parked" in T cell-depleted mice, tolerance and "resistance" were eventually lost by 6 months. (ox.ac.uk)

Whole-body retention was followed by external gamma counting, and particle clearance kinetics were determined by excretion radioanalysis. (cdc.gov)

In conclusion, Vero cells were grown on Cytodex 1 with serum-free media and a high amount of rabies virus was obtained. (researchsquare.com)
whole well image of culture well (96 well plate format) containing human induced pluripotent stem cells grown on mouse feeders, stained for pluripotent markers NANOG (red) and OCT4 (green). (lu.se)

Adipose tissue (AT) represents a commonly used source of mesenchymal stem/stromal cells (MSCs) whose proregenerative potential has been widely investigated in multiple clinical trials worldwide. (mdpi.com)
This lightweight, fast-absorbing serum, powered by a fusion of sweet clover stem cells and plant-derived beta hydroxy acid, instantly unclogs and minimizes the appearance of pores. (aveda.com)
Over 10,000 stem cells are infused into each serum to help purify and tighten pores, and helps increase epidermal thickness. (aveda.com)
We aimed to compare DPSC and SHED as stem cell sources with a future use in regeneration of calcified tissue . (bvsalud.org)
Both DPSC and SHED expressed stem cell markers and were able to differentiate into bone , fat and cartilage like cells . (bvsalud.org)
A subpopulation of cells within a cancer has the properties of stem cells. (msdmanuals.com)

IHC analysis of Pax7 and neural cell adhesion molecule (NCAM) appeared to sufficiently and similarly represent the expansion of SCs after injury (28-36% increase). (mcmaster.ca)

Why do most enzymes in a living cell work with substrate concentrations below Km? (flashcardmachine.com)

Cell-surface receptors are the targets for more than 60% of current drugs. (nih.gov)
A multiscale anatomy of carcinoma will reveal that the microstructure of cancer cells contains some characteristic elements such as specific biomarker receptors and DNA molecules that exclusively differentiate them from healthy cells. (imechanica.org)

What are the assumptions of Michaelis-Menten Enzyme Kinetics? (flashcardmachine.com)

The lifetime of the rCD2/rCD48T92A interaction was also investigated using single-molecule imaging and tracking displaying a similarly small lifetime spread within the cell population. (lu.se)
Later, the studies focused on the CD4-TCR-MHC II ternary complex where it was demonstrated that the presence of L3-12 TCR strongly supported the CD4-MHC II interaction by increasing the local density of MHC II inside the cell-SLB contacts. (lu.se)
The functional interaction of cells in the formation of tissues requires the establishment and maintenance of cell-cell contact by the junctional complex. (silverchair.com)

The first-in-human testing of scFv-based B7-H3 CAR-T cells has shown antitumor response against anaplastic meningioma and childhood diffuse intrinsic pontine glioma without severe side effects 16 , 17 . (nature.com)
The type I/III IFN pathways are directly involved in protecting neighboring cells from virus spread and are vital for the immediate cell-intrinsic antiviral response. (nature.com)

Two-fold serum dilutions (starting dilution of 1:10) were incubated with 40-60 PFUs of virus for 1 h at 37°C. The virus-serum mixture was added onto the Vero cells monolayer for 1hr at 37°C in a 5% CO incubator. (cdc.gov)
Adherent Vero cells can be used in different kinds of microcarriers for industrial production in cell culture type bioreactors. (researchsquare.com)

The association kinetics were better explained by a model with a minimal encounter duration rather than a standard on-rate constant, suggesting that membrane fluidity and dynamics may strongly influence bond formation. (ox.ac.uk)

On the basis of bio-chemical kinetics, we present a model of muscle fiber exhaustion based on hydrolytic ATP-ADP-P i dynamics, which are assumed to be length- and calcium activity-dependent. (frontiersin.org)

Small tumors have a greater percentage of actively dividing cells than do large tumors. (msdmanuals.com)

A stochastic epigenetic switch controls the dynamics of T-cell lineage commitment. (crossref.org)

We have studied lung retention and clearance kinetics in 12 healthy male adult WKY rats up to 6 mo after an inhalation of 192Ir-radiolabeled, insoluble, ultrafine 15- to 20-nm iridium particles. (cdc.gov)

During virus infection, the specific immune signals produced from infected cells are important for dictating the recruitment and activation of innate or adaptive immune cells that are required to fight virus infection. (nature.com)
During early infection, the neutrophil count may actually decrease briefly because of margination of cells from the blood. (medscape.com)
Cell infection, at an MOI of 0.3 with serum-free medium conditions, yielded a maximal rabies virus titer of 1.82×10 7 FFU/mL at 5 days. (researchsquare.com)

60. Sulaiman OA, Gordon T. Role of chronic Schwann cell denervation in poor functional recovery following nerve injuries and experimental strategies to combat it. (jnkinhibitor.com)

abstract = "It has been determined previously that polymorphonuclear leukocytes, or PMNs, can facilitate melanoma cell extravasation through the endothelium under shear conditions [1,2]. (psu.edu)
abstract = "Regulatory volume decrease (RVD) is a process by which cells restore their original volume in response to swelling. (elsevierpure.com)

However, the successful application of these emerging cell-based therapies in solid tumors remains limited. (nature.com)
However, surgically-removed tumors often leave behind a residual cancer cell population. (imechanica.org)

Id2a-deficient retinas possess increased numbers of cells occupying S phase, at the expense of mitotic cells, and kinetic analyses demonstrate that Id2a is required for S-phase progression and/or the transition from S to M phase. (biologists.com)

Anatomy13

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Chemicals and Drugs37

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes52

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Information Science4

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Binding Kinetics4

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Humans2

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Mechanisms7

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Proteins5

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