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  • vitro
  • In vitro studies in HL-1 cardiomyocytes have shown that inhibition of desmoglein-2 binding or mutation of desmoglein-2 protein (Ala517Val or Val920Gly) at cardiac intercalated discs results in a reduced strength of cell-cell contact, demonstrating that desmoglein-2 is critical for cardiomyocyte cohesion. (wikipedia.org)
  • In Vitro Cell. (wikipedia.org)
  • A cell-free system is an in vitro tool widely used to study biological reactions that happen within cells apart from a full cell system, thus reducing the complex interactions typically found when working in a whole cell. (wikipedia.org)
  • reaction
  • These systems have enabled cell-free synthetic biology to emerge, providing control over what reaction is being examined, as well as its yield, and lessening the considerations otherwise invoked when working with more sensitive live cells. (wikipedia.org)
  • In this example the labelling reaction (reductive dimethylation) also blocks the reactive amino groups. (wikipedia.org)
  • cellular
  • Our approach can be extended to other cellular compartments and cell states, and our data provide the first broad biochemical framework for understanding C phase. (mcponline.org)
  • We applied this workflow to Plk1 (Polo-like kinase 1) in mitotic cells and induced cellular Plk1 activity by wash-out of the bulky kinase inhibitor 3-MB-PP1, which targets a mutant kinase version with an enlarged catalytic pocket while not interfering with wild-type Plk1. (mcponline.org)
  • tumor
  • LCM allowed for the one-step procurement of homogeneous populations of normal/tumor epithelial cells from tissue sections. (aacrjournals.org)
  • Following transplant of melanoma cells into zebrafish, we will follow metabolite exchange from tumor to stroma (or vice versa) by tracking isotope labels with both LC/MS and NIMS in situ imaging. (cancer.gov)
  • Its role in coupling ATP from oxidative phosphorylation to the rate-limiting step of glycolysis may help drive the tumor cells' growth. (wikipedia.org)
  • The CTRP5 protein is a member of the C1q / tumor necrosis factor superfamily, which shows diverse functions including cell adhesion and as components of the basement membrane. (wikipedia.org)
  • nonspecific
  • The existence of coexpressed gene clusters that lack a functional connection is intriguing given that nonspecific gene expression should have a negative impact on cell fitness. (mcponline.org)
  • Biology
  • Even in the case of the lightest elements where the ratio of neutron number to atomic number varies the most between isotopes it usually has only a small effect, although it does matter in some circumstances (for hydrogen, the lightest element, the isotope effect is large enough to strongly affect biology). (wikipedia.org)
  • bacteria
  • Previously, QconCATs have been expressed in bacteria, but we now describe QconCAT expression in a robust, cell-free system. (mcponline.org)
  • coli
  • E. coli, wheat germ, and rabbit reticulocytes have all proven useful to create cell-free systems by extraction of their interior components. (wikipedia.org)
  • substrate
  • The second definition applies specifically to the full substrate repertoire of a certain protease in a cell, tissue, or organism. (wikipedia.org)