Cell in vitro cell clone clone cells. Medical search. Frequent questions

Here we describe the cloning of two Afghan hounds by nuclear transfer from adult skin cells into oocytes that had matured in vivo . (nature.com)
We transfected human and rabbit peripheral blond mononaclear cells (PBMC) with the ACH molecular clone of human T-cell lymphotropic virus type 1 (HTLV-1) to study its in vitro and in vivo properties. (wustl.edu)
This clone will facilitate investigations into the role of viral genes on biological properties of HTLV-1 in vitro and in vivo. (wustl.edu)
Furthermore, POGLUT2 knockdown of breast cancer cells was established, followed by in vitro biological function assays and in vivo tumor growth study. (frontiersin.org)
In vitro and in vivo results showed that POGLUT2 knockdown could delay tumor growth and progression. (frontiersin.org)
In this review, we discuss the rationale for MSC-based cell therapy in COPD, the main findings from in vitro and in vivo preclinical COPD model studies, clinical trials in patients with COPD and directions for further research. (bmj.com)
The major breakthrough is the identification of a biomarker of HIV persistent cells in vivo. (europa.eu)
MSCs were also investigated for stem cell qualities in vivo. (hindawi.com)
The 1A29 antibody can reportedly inhibit leucocyte infiltration in in vivo systems, blocks induction of experimental allergic encephalomyelitis, and reduces NK-cell adhesion to tumor cells. (bdbiosciences.com)
To better understand Plasmodium biology, researchers can whether proceed to in vitro studies or use in vivo models. (wjgnet.com)
To compare drug sensitivity of ALL cells from diagnosis to relapse in vitro and in vivo and determine if acquired resistance to specific agents is related to specific somatic genome variants that are not detected or found in only a minor clone at initial diagnosis. (centerwatch.com)
CSC-like cells were isolated using fluorescence-activated cell sorting and were assessed for aggressive behaviors, including acquired apoptosis resistance and increased cell migration and invasion in vitro, and tumor-initiating capability in vivo. (cdc.gov)
These CSC-like cells, in contrary to their non-CSC counterpart, possessed all biological features of lung CSC that are central to irreversible malignant transformation, self-renewal, aggressive cancer behaviors, and in vivo tumorigenesis. (cdc.gov)
In vitro and in vivo experiments were conducted to investigate the biological functions of LINC01572 in tumorigenesis and development. (bvsalud.org)
Relating in vitro to in vivo exposures with physiologically based tissue dosimetry and tissue response models. (cdc.gov)
This project will delve into innate immune cell function, establishing in vitro and in vivo systems test innate immune cell function in regeneration. (lu.se)
Inspired from developmental processes, human mesenchymal cell lines can be programmed to form cartilage, bone and bone marrow tissues in vitro and in vivo. (lu.se)

it helped uncover the secrets of cancer and the effects of the atom bomb, and led to important advances like cloning, in vitro fertilization, and gene mapping. (oprah.com)
The Johns Hopkins researchers' system involves the study of existing embryonic stem cell lines derived from in vitro fertilization methods, and so doesn't require generation of embryos through cloning, a technique recently reported by South Korean scientists. (sciencedaily.com)
This paper gives an Islamic perspective on some of these advances, including abortion, in vitro fertilization, genetic engineering, cloning and stem cell research. (who.int)

Even though in vitro gametogenesis could serve as a powerful new technology to overcome infertility, I argue that opponents of the practice of creating embryos solely for research purposes would still view the creation of research embryos that the development of in vitro gametogenesis would require, as being incompatible with affording the embryo proper moral respect. (bmj.com)
Much research has been devoted to the development of in vitro and in silico predictive testing methods. (cdc.gov)

5 Functional in vitro assays indicate different physiological roles of MSCs related to their heterogeneity and tissue location of origin. (bmj.com)
These included colony formation assays, cell migration assays, flow cytometry, cell counting kit-8 (CCK-8) cell proliferation and tumor transplant growth experiments. (bvsalud.org)

Following early observations that MSCs inhibit T-cell proliferation, 9 MSCs were found to interact with the majority of innate and adaptive immune cells. (bmj.com)
Human bmMSCs were described in the late nineties as well [ 6 ] and at the same time a breakthrough study investigated the expression of typical cell surface markers and the proliferation and differentiation properties of human MSCs in more detail [ 7 ]. (hindawi.com)
This clone can suppress anti-CD3 induced T cell proliferation in vitro based on in-house testing. (biolegend.com)
METHODS: A549 and LLC cell lines treated with 5 or 10 mmol/L BHB were examined for changes in cell viability, proliferation, migration, and invasion using CCK-8 assay, EdU staining, scratch assay, and Transwell assay. (bvsalud.org)
RESULTS: Treatment with BHB concentration-dependently repressed the viability, proliferation, migration and invasion of A549 and LLC cells. (bvsalud.org)
Inhibition of LINC01572 expression significantly impaired cell proliferation, cell migration, and clone formation. (bvsalud.org)
On average, about 2% to 6% of beryllium-exposed people develop beryllium sensitization (defined by positive blood lymphocyte proliferation to beryllium salts in vitro), with most sensitized people progressing to disease. (msdmanuals.com)

Expression, gene mutation and amplification, methylation, and copy number alteration (CNA) of POGLUT2 were evaluated using The Cancer Genome Atlas (TCGA), Cancer Cell Line Encyclopedia (CCLE), and Genotype-Tissue Expression (GTEx) databases. (frontiersin.org)
Due to constant research and development activities in the stem cell treatment market there has been a growth in the demand for the gene treatments and the cell treatments with the help of the stem cells. (precedenceresearch.com)
The following GMFG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
In addition, gene set enrichment analysis (GSEA) and immune landscape profiling showed that LINC01572 can promote tumor initiation and progression by deregulating the cell cycle and immunocyte infiltration. (bvsalud.org)
2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)

In 2001, Lorenz Studer, MD , Head of the Stem Cell and Tumor Biology Laboratory at MSKCC, and his colleagues at Rockefeller University published research in which they generated unlimited numbers of genetically matched dopamine nerve cells using cloned stem cells whose genetic material originated from the mouse's own tail. (mskcc.org)
Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. (cellsignal.com)
Recombinant antibodies are monoclonal, but their production involves in vitro genetic manipulation. (cellsignal.com)
A clone is an organism that is a genetic copy of an existing one. (who.int)

A ribophorin I cDNA clone containing a 0.6-kb insert was isolated from a rat liver lambda gtll cDNA library by immunoscreening with specific antibodies. (rupress.org)
This cDNA was used to isolate a clone (2.3 kb) from a rat brain lambda gtll cDNA library that contains the entire ribophorin I coding sequence. (rupress.org)
This confirmed the identity of the cDNA clone and established that ribophorin I contains 583 amino acids and is synthesized with a cleavable amino terminal insertion signal of 22 residues. (rupress.org)
Hybridization measurements using the cloned cDNA as a probe showed that ribophorin I mRNA levels increase fourfold 15 h after partial hepatectomy, in confirmation of measurements made by in vitro translation of liver mRNA. (rupress.org)
Characterization of adenylyl cyclases has been facilitated by the isolation of cDNA clones for distinct adenylyl cyclases including the type I and type III enzymes. (nih.gov)

Increased levels of POGLUT2 were found in multiple types of cancer tissues and cell lines. (frontiersin.org)
hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
This new method of generating stem cells does not require embryos as starting points and could be used to generate cells from many adult tissues, such as a patient's own skin cells,' said principal author Andras Nagy, senior investigator at Mount Sinai's Samuel Lunenfeld Research Institute. (cbc.ca)
Recently, MSCs isolated from bone marrow (bmMSCs) were shown to be a blend of distinct cells and MSCs isolated from different tissues show besides some common features also some significant differences. (hindawi.com)
It is expressed on vascular endothelium in lymphoid tissues, thymic stromal cells, peripheral blood monocytes, peritoneal macrophages and mast cells, dendritic cells, and weakly on peripheral lymphocytes and thymocytes. (bdbiosciences.com)
In this study, knockdown of GMF-gamma by RNA interference enhanced actin polymerization and contraction in human airway smooth muscle (HASM) cells and tissues without affecting myosin phosphorylation. (genscript.com)

is associated with colorectal cancer metastasis and its downregulation suppresses colorectal cancer cell migration and invasion in vitro. (genscript.com)

The company worked on what they called the "Ji Gami" process, which involved the removal of a small strip of the scalp, which is broken down into individual follicular stem cells. (wikipedia.org)
Scientists hoped that after implantation these cloned follicular cells would mature into full-grown hair. (wikipedia.org)
TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. (biolegend.com)
Activated T cells, Regulatory T cells (Treg), Follicular Helper T cells (TFH), NK cells. (biolegend.com)
It is a single-chain membrane glycoprotein of approximately 200 kd that has 4 allotypic forms on myeloid cells (eg, erythrocytes, granulocytes, monocytes), lymphoid cells (mostly B cells), follicular dendritic cells, and glomerular podocytes. (medscape.com)

POGLUT2 was mainly expressed in stromal cells as verified by StromalScore, ESTIMATEScore, ImmuneScore, and Tumor purity, and POGLUT2 was positively correlated with cancer-associated fibroblasts, macrophages, monocytes, and neutrophils in the tumor microenvironment. (frontiersin.org)
Mesenchymal stromal cells: a novel therapy for the treatment of chronic obstructive pulmonary disease? (bmj.com)
In vitro cell culture and animal studies have demonstrated that mesenchymal stromal cells (MSCs) have the capacity to modify immune responses and to enhance tissue repair. (bmj.com)
Mesenchymal stromal cells (MSCs) are cells of non-haematopoietic origin, with the capacity to differentiate into multiple lineages of the mesenchyme, that is, chondrocytes, osteoblasts and adipocytes. (bmj.com)
However, transplanting other than hematopoietic stem and progenitor cells is still limited to a few applications, and it mainly applies to mesenchymal stromal cells (MSCs) isolated from bone marrow. (hindawi.com)
Nowadays, experts agree that MSCs may generate upon appropriate stimulation quite different mature cells including osteoblasts, chondrocytes, tenocytes, adipocytes, smooth muscle cells, and stromal cells of the bone marrow [ 9 ]. (hindawi.com)

But previous approaches required the use of viruses to deliver the four genes needed to activate the cell and accomplish that task. (cbc.ca)
Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.com)
More and more we're learning that the genes that turn on in the embryo to make blood stem cells are the same genes that go wrong in cancer," he says. (sciencedaily.com)
Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)

The first clinical trials involving a patient receiving human embryonic stem cells began in October 2010 at the Shepard Center, a spinal cord injury hospital in Atlanta. (cbc.ca)
Scientists say embryonic stem cells are the most useful type because they have the potential to become any type of cell within the body. (cbc.ca)
However, researchers at the Harvard Stem Cell Institute say reprogrammed cells won't eliminate the need or value of studying embryonic stem cells. (cbc.ca)
Stem cells come in three forms: embryonic stem cells, embryonic germ cells and adult stem cells. (cbc.ca)
Embryonic stem cells come from embryos, embryonic germ cells from testes, and adult stem cells can come from bone marrow. (cbc.ca)
Embryonic stem cells are pluripotent - they have the ability to become virtually any type of cell within the body. (cbc.ca)
Furthermore, embryonic stem cells can form a whole new organ. (precedenceresearch.com)
I claim that the debate over so-called compromise positions in the human embryonic stem cell debate suggests that the purpose of the research for which a research embryo is created is unlikely to be considered as having any significant bearing on the moral permissibility of the practice for those who oppose it. (bmj.com)
Johns Hopkins scientists have developed a way to study the earliest steps of human blood development using human embryonic stem cells grown in a lab dish instead of the embryos themselves. (sciencedaily.com)
In their report on the work in the June issue of the journal Blood, the Johns Hopkins team demonstrated a clear similarity between how human embryonic stem cells specialize into blood cells and how blood cells develop in human embryos. (sciencedaily.com)
Because embryonic stem cells are capable of becoming virtually every type of cell in the human body, understanding how they do so might provide the chance to harness that process to make a limitless supply of specific cells for therapeutic purposes. (sciencedaily.com)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)

To understand the etiology of osteopetrosis, it is very essential to understand the bone-remodeling cycle and the cell biology of osteoclasts. (medscape.com)
In 1999, Baron clearly and concisely reviewed the cell biology of the bone remodeling. (medscape.com)

Thanks to recent progresses, stem cells have been extensively employed to study Plasmodium liver and blood cycle in vitro . (wjgnet.com)
Finally, some of the cells in the colonies form blood cells similar to those found in the liver and bone marrow of a developing fetus, making it simple for the researchers to pick out the blood cells for further investigation. (sciencedaily.com)
Zambidis and colleagues are currently using their model to study the next stage in blood cell development, which in a growing embryo involves blood cell precursors moving from the yolk sac into the liver, bone marrow and thymus. (sciencedaily.com)
Opsonized immune complexes (coated by C3b and C4b) bind to CR1, mostly on red blood cells, and are cleared through the liver where they can be transferred to CR3-bearing phagocytes and endocytosed. (medscape.com)

SP6 RNA transcripts of the insert in this clone directed the in vitro synthesis of a polypeptide of the expected size that was immunoprecipitated with anti-ribophorin I antibodies. (rupress.org)
In atopic individuals, sensitizing T-cell epitopes can trigger a cascade of events that leads to synthesis of allergen-specific immunoglobulin E (IgE) antibodies as well as other immunological reactions. (ispub.com)
TotalSeq™-D antibodies are compatible with Mission Bio's Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein. (biolegend.com)
While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.com)
In vitro methods for producing antibodies are amenable to large-scale production, meaning antibody availability is unlikely to become a limiting factor. (cellsignal.com)
Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.com)
cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)

Here, we report the establishment and the characteristics of persistent in vitro infection of human hepatoma-derived cells by a recently described HCV genotype 2a infectious molecular clone. (nih.gov)

Protein activation and expression were analysed by western blotting, deoxyribonucleoside triphosphate levels by HPLC, ribonucleotide reductase activity by 14 C-cytidine incorporation into nascent DNA and cell-cycle distribution by FACS. (nature.com)
Expression of type I adenylyl cyclase activity in animal cells has established that this enzyme is stimulated by calmodulin and Ca2+. (nih.gov)
Its expression is upregulated on activated lymphocytes and endothelial cells. (bdbiosciences.com)
Expression of TIGIT is associated with decreased functionality of CD8 T cells in chronic viral infection and tumors. (biolegend.com)
TotalSeq™-D reagents are designed to profile protein expression at single cell level. (biolegend.com)
ORF sequences can be delivered in our standard vector, pcDNA3.1 + /C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. (genscript.com)
Restored expression of tumor suppressor p53 abrogated CSC properties of CSC-like cells. (cdc.gov)
Among healthy individuals, the number of CR1 receptors on the red blood cells varies widely, yet the phenotypic expression, regulated by 2 codominant alleles, is stable. (medscape.com)
B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)

2002). In humans, SSEA4 is expressed by building the nervous system but also for their prospec- nonneural cells such as the erythrocytes (Kannagi et al. (lu.se)

The abnormal behavior of these follicles is suggested to be the result of progenitor cell deficiency in these areas. (wikipedia.org)
We know that innate immune cells are required for blastema progenitor cell formation, but are lacking an understanding of the immune cell processes and functions that are required for inducing and maintaining these progenitors cells. (lu.se)

Potential allergenicity of a protein is a complex issue and various tests can be made for the prediction, includes in silico as well as in vitro digestibility and binding antisera of allergic patients. (ispub.com)

They display differentiation capacities and therefore qualify as multipotent progenitor cells (Figure 1 ). (hindawi.com)
Overview on self-renewal or differentiation of stem cells in their respective stem cell niche. (hindawi.com)
Changes in the differentiation potential of MSCs after in vitro expansion were noted and chondrogenic clones especially disappeared early on [ 12 ]. (hindawi.com)
TIGIT also promotes the differentiation of tolerogenic phenotype in dendritic cells with an increased secretion of IL-10 and a diminished production of IL-12. (biolegend.com)
We've captured these phases of stem cell specialization, or differentiation, in a dish," says Zambidis. (sciencedaily.com)

Researchers there are working on technology that induces human skin cells to change into the kind of stem cells that have been created by embryos. (cbc.ca)
Some argue that the possibility of mimicking stem cells without acquiring them from embryos, side-steps that moral dilemma. (cbc.ca)
I conclude by suggesting that Sparrow's analysis of the potential benefits of in vitro gametogenesis provides us with further reasons to scrutinise the unconvincing arguments that are often cited in favour of prohibiting the practice of creating embryos solely for research purposes. (bmj.com)
Reprogramming was demonstrated by the capacity of the reconstructed embryos to develop to the blastocyst stage in vitro and into fetuses and viable offspring in suitable foster mothers. (cnrs.fr)
Historically, scientists have worked on mouse and zebrafish models of embryological blood cell development, but ethical and technical barriers have stood in the way of an in-depth study of blood formation in human embryos. (sciencedaily.com)
These colonies can then also form the precursors of blood cells, in a structure similar to the yolk sac of human embryos. (sciencedaily.com)
Most importantly, Zambidis says, the stages of blood cell development he and his team found in the stem cell lines correlate with what is already known about early stages of human blood cell development in embryos in the womb. (sciencedaily.com)
This is currently the most popular method for the generation of targeted knock-out and knock-in models by ES-cell injections into 8-cell morulae and/or blastocysts of mouse embryos. (lu.se)

To our knowledge, this is the first report of cloned mammalian offspring originating from nonviable cells. (cnrs.fr)
Mammalian cell lines are most commonly used for recombinant antibody production, although cell lines of bacterial, yeast, or insect origin are also suitable. (cellsignal.com)

Clone G146-254 has been shown to recognize in vitro translated, recombinant bacterially expressed, and endogenous B cell Fli-1. (bdbiosciences.com)

They can be multiplied (cloned) by various culturing methods and the new cells can be injected back into the bald scalp, where they would produce healthy hair. (wikipedia.org)
In the 5% strength, it is also useful in the treatment of superficial basal cell carcinomas when conventional methods are impractical, such as with multiple lesions or difficult treatment sites. (nih.gov)
Methods: Non-tumorigenic human lung epithelial cells were chronically exposed to well-dispersed SWCNT for a period of 6 months at the physiologically relevant concentration of 0.02 mu g/cm(2) surface area dose. (cdc.gov)

Named "HeLa" (short for Henrietta Lacks), the first "immortal" human cells grown in culture are still alive today. (depauw.edu)
In fact, an estimated 50 million metric tons of HeLa cells have been grown, with a combined weight, says Skloot, of the equivalent of more than 150 Empire State Buildings. (depauw.edu)
Confocal immunofluorescent analysis of HeLa cells, insulin-treated (left) and LY294002-treated (#9901, right), using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP ® Rabbit mAb (Alexa Fluor 488 ® Conjugate) (green). (cellsignal.com)
A scientist put that sample into a test tube, and, though Henrietta died eight months later, her cells-known worldwide as HeLa-are still alive today. (oprah.com)
Since 2001 alone, five Nobel Prizes have been awarded for research involving HeLa cells. (oprah.com)
One scientist estimates that if you could pile all the HeLa cells ever grown onto a scale, they'd weigh more than 50 million metric tons-the equivalent of at least 100 Empire State Buildings. (oprah.com)

Mice with C5a receptor-deficient dendritic cells promote induction of Treg and Th17 cells. (medscape.com)

Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)

1. Major histocompatibility complex class II antigens have the central role in the immune response of 'presenting' antigenic peptide to CD4 + T-cells. (portlandpress.com)
Because of their high numbers, red blood cells express an average of 90% of the CR1 receptors despite their having a lower number of antigenic sites per cell. (medscape.com)

2 MSCs were first described in the bone marrow where they constitute a small fraction of cells (0.001%-0.01%) that closely interact with haematopoietic cells to support haematopoiesis and skeletal homeostasis. (bmj.com)
Today, more than 50 years after the first successful bone marrow transplantation, clinical application of hematopoietic stem cells is a routine procedure, saving the lives of many every day. (hindawi.com)
The MSCs have been described for the first time as colony forming fibroblasts (CFU-F), a rare population of cells residing in the bone marrow of guinea-pigs or mice [ 1 , 2 ]. (hindawi.com)
It seems that the term "stem cell" became more popular although the "stemness" was only shown in a more strict sense for MSCs involved in osteogenesis and bone repair. (hindawi.com)

fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)

The kit also allows direct cloning from amplification reactions without purification, and works well whether or not the primers used in the PCR possess 5´-phosphate groups. (neb.com)

NEW YORK, September 21, 2003 - New research from Memorial Sloan Kettering Cancer Center (MSKCC), Cornell University, and The University of Connecticut describes a novel way of producing therapeutic nerve cells that can cure mice with Parkinson's-like disease. (mskcc.org)
Zambidis says that if blood stem cells are to be used for therapeutic purposes, they would likely come from this next stage of development. (sciencedaily.com)
5. In 2001, France and Germany requested the United Nations General Assembly to develop international conventions on human reproductive cloning, therapeutic cloning and research on stem cells. (who.int)

1993. In vitro percutaneous absorption and metabolism in man of 2-chloro-4-ethylamino-6-isopropylamine-s-triazine (atrazine). (cdc.gov)

A clinical isolate hCoV-19/Italy/UniSR1/2020 (GISAID accession ID: EPI_ISL_413489) was isolated and propagated in Vero E6 cells, and viral titer was determined by 50% tissue culture infective dose (TCID 50 ) and plaque assay for confirming the obtained titer. (frontiersin.org)
An aliquot (0.8 mL) of the transport medium of the nasopharyngeal swab (COPAN's kit UTM® universal viral transport medium-COPAN) of a mildly symptomatic SARS-CoV-2 infected patient was mixed with an equal volume of DMEM without FBS and supplemented with double concentration of P/S and Amphotericin B. The mixture was added to 80% confluent Vero E6 cells monolayer seeded into a 25 cm 2 tissue culture flask. (frontiersin.org)
Persistent in vitro infection was characterized by the selection of viral variants that displayed accelerated expansion kinetics, higher peak titers, and increased buoyant densities. (nih.gov)
In parallel, as the virus became more aggressive, cells that were resistant to infection emerged, displaying escape mechanisms operative at the level of viral entry, HCV RNA replication, or both. (nih.gov)
show that this approach can detect characteristic changes that affect blood cells as a result of malaria, spherocytosis, bacterial and viral infections, and leukemia. (elifesciences.org)

When the biological properties of MSCs were explored in more detail, questions arose whether these cells met the criterion of a true stem cell [ 8 ]. (hindawi.com)
1. Cloning is an umbrella term traditionally used to describe different processes for duplicating biological material. (who.int)

1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

We tested whether the cloned dogs were genetically identical by microsatellite analysis of genomic DNA from the donor Afghan, the cloned dogs and the surrogates (see supplementary information ). (nature.com)
Analysis of eight canine-specific microsatellite loci confirmed that the cloned dogs were genetically identical to their donor dog. (nature.com)

In addition to the new in vitro systems, in recent years there were also significant advances in the development of new animal models that allows studying the entire cell cycle of human malaria. (wjgnet.com)

Donor fibroblasts were obtained from an ear-skin biopsy of a male Afghan hound and cultured for two to five passages (in which fully grown cells are transferred to a new culture dish). (nature.com)
This therapy would also eliminate the need for donor hair, as it can be simply grown from the patient's own cells. (wikipedia.org)
The first 'immortal' human cells grown in culture, they are still alive today, though she has been dead for more than sixty years. (worldcat.org)
There has been a constant growth in the research associated with the stem cell activities and the number of approval start come from the authorities have increased due to which the market for the stem cell therapy has grown well in the past and it is expected to grow during the forecast period. (precedenceresearch.com)
In the new work, Hopkins scientists and colleagues from the University of Pittsburgh School of Medicine used laboratory-grown dishes of human stem cells, in clumps called human embryoid bodies, and observed three distinct steps taken by stem cells on their way to becoming blood cells. (sciencedaily.com)

Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
In a study published in the online journal Nature on March 1, 2009, Canadian researches described a new method for generating stem cells from adult human tissue. (cbc.ca)
Stem cells are found in human body in an early stage of life as well as in adults and can differentiate into specialized cells types of a tissue or an organ. (precedenceresearch.com)
This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P). (biolegend.com)
Without any chemical manipulation or stimulation, the clusters of human stem cells first became colonies of cells that can produce endothelium, or the tissue that makes up the circulatory system. (sciencedaily.com)
Further study of clinical specimens confirmed that LINC01572 was highly expressed in the tumor tissue of non-small cell lung cancer (NSCLC) patients. (bvsalud.org)

As a result, they produced follicles "thinner than normal", but they were confident they could develop the right method of cloning hair from human stem cells by 2011. (wikipedia.org)

To overcome this problem and to improve life expectancies of cancer patients, researchers are constantly working on stem cell therapy. (precedenceresearch.com)
Knowing the steps by which stem cells develop into blood cells are likely to help medical researchers figure out how to treat cancers of the blood, such as leukemia and lymphoma, Zambidis notes. (sciencedaily.com)

The density of CR1 receptors on cell surfaces varies with the cell type and with the activation of the cell for neutrophils and monocytes. (medscape.com)

Oncolytic viruses have an inherent or acquired selectivity to replicate exclusively in tumor cells, ultimately destroying them. (helsinki.fi)

These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)

At the same time, by asymmetric cell division or after specific activation, stem cells must be able to generate more mature progenitor cells or differentiated effector cells (Figure 1 ). (hindawi.com)

In vitro experiments also confirmed that LINC01572 was overexpressed in tumor cell lines. (bvsalud.org)

These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)

Calu-3 (Human lung cancer cell line, ATCC HTB-55) were cultured in MEM supplemented with NEAA (1x), P/S (100 U/mL), HEPES buffer (10 mM), and 10% (v/v) FBS. (frontiersin.org)
Induction of stem-like cells with malignant properties by chronic exposure of human lung epithelial cells to single-walled carbon nanotube s. (cdc.gov)
Non-small cell lung cancer cells served as a positive control. (cdc.gov)
Results: We demonstrated for the first time the existence of CSC-like cells in all clones of chronic SWCNT-exposed lung epithelial cells. (cdc.gov)
OBJECTIVE: To explore the mechanism that mediates the inhibitory effects of ß-hydroxybutyrate (BHB) on lung adenocarcinoma cells. (bvsalud.org)
GPR109A expressions in BHB-treated lung adenocarcinoma cells were determined using RT-PCR and Western blotting. (bvsalud.org)
RESULTS: Bioinformatics results showed that LINC01572 was overexpressed in both LUAD and lung squamous cell carcinoma (LUSC) patients. (bvsalud.org)

While the SARS-CoV-2 pandemic is heavily hitting the world, it is of extreme importance that significant in vitro observations guide the quick set up of clinical trials. (frontiersin.org)
For treating the virus, the stem cells have been used for various clinical trials. (precedenceresearch.com)

2. Over the years, the international community has tried without success to build a consensus on an international convention against the reproductive cloning of human beings. (who.int)
3. Creating awareness among ministries of health in the African Region will provide them with critical and relevant information on the reproductive cloning of human beings and its implications to the health status of the general population. (who.int)
7. The WHO Regional Committee for Africa is invited to review this document for information and guidance concerning reproductive cloning of human beings. (who.int)
3. Media reports on nuclear transfer are usually about one form, reproductive nuclear transfer, also known as reproductive cloning of human beings . (who.int)

T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3 or WUCAM, is a 26 kD, type I transmembrane protein and is a member of the PVR (poliovirus receptor) family of immunoglobulin-like domain containing proteins. (biolegend.com)
This interaction with a T-cell's receptor may result in activation, but, if recognition occurs without collateral molecular interactions which cause 'co-stimulation', these T-cells will be tolerized. (portlandpress.com)
CR1 and CR2 have been shown to influence the immune environment in a B-cell receptor-independent manner. (medscape.com)

Successful somatic-cell nuclear transfer (SCNT) depends on the quality, availability and maturation of the animal's unfertilized oocytes. (nature.com)
Figure 1: Dog cloned by somatic-cell nuclear transfer. (nature.com)
a , Snuppy, the first cloned dog, at 67 days after birth (right), with the three-year-old male Afghan hound (left) whose somatic skin cells were used to clone him. (nature.com)
Here we report on the successful reprogramming of nuclei from somatic cells rendered nonviable by heat treatment. (cnrs.fr)
To identify germline or somatic genomic variants associated with drug resistance of ALL cells to conventional and newer targeted anti-leukemic agents in a non-clinical, research setting. (centerwatch.com)

Our aim was to evaluate the frequency of mTOR and ERK pathway upregulation in a retrospective series of 139 ITAC and to test the efficacy and mechanism of action of candidate targeted inhibitors in cell line ITAC-3. (bvsalud.org)

Expansion of MSCs was shown to be limited to a few passages of in vitro culture and the cells underwent replicative senescence [ 11 ]. (hindawi.com)

Furthermore, we identified specific stem cell surface markers CD24(low) and CD133(high) that are associated with SWCNT-induced CSC formation and tumorigenesis. (cdc.gov)
2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)

Adult stem cells are more limited. (cbc.ca)
Granulosa cells from adult sheep were heated to nonphysiological temperatures (55 degrees C or 75 degrees C) before their nuclei were injected into enucleated metaphase II oocytes. (cnrs.fr)

Though those cells have done wonders for science, Henrietta-whose legacy involves the birth of bioethics and the grim history of experimentation on African-Americans-is all but forgotten. (oprah.com)

Anatomy30

Organisms23

Diseases13

Chemicals and Drugs73

Analytical, Diagnostic and Therapeutic Techniques and Equipment45

Phenomena and Processes76

Disciplines and Occupations1

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Vivo17

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19931

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