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  • proteins
  • It is the soluble and insoluble compositions of multipotent proteins and growth factors which make up Histogen's products. (prweb.com)
  • Through this unique technology process, newborn cells are encouraged to naturally produce the vital proteins and growth factors from which the Company has developed its rich product portfolio. (prweb.com)
  • Histogen's technology focuses on stimulating a patient's own stem cells by delivering a proprietary complex of multipotent human proteins that have been shown to support stem cell growth and differentiation. (prweb.com)
  • The fight against infections prompts creation of memory T cells that then "remember" antigens or proteins found on cells infected with viruses or bacteria. (medindia.net)
  • Orderly progression from one phase of the cell cycle to the other is mediated mainly by the regulation of gene expression, and key factors of this process are often transcriptional regulatory proteins. (embopress.org)
  • Low molecular weight of epidermal growth factor (EGF) enables better intratumoral penetration in comparison with larger targeting proteins, but the cellular retention of EGF-associated radioactivity is poor for directly iodinated EGF. (diva-portal.org)
  • A new method for post-translationally labeling proteins in live cells for fluorescence imaging and tracking. (nih.gov)
  • Cell division in bacteria is controlled by the septal ring, a collection of about a dozen proteins that collect around the site of division. (bionity.com)
  • Despite general similarities between mDCs and the model systems, moDCs and KG-1 cells, our findings identified some significant differences in the proteomes of these cells, and the findings were confirmed by ELISA detection of a selection of proteins. (biomedcentral.com)
  • This was particularly noticeable with proteins involved in cell growth and maintenance (for example, fibrinogen γ chain (FGG) and ubiquinol cytochrome c) and cell-cell interaction and integrity (for example, fascin and actin). (biomedcentral.com)
  • Proteins are functional units of cells that assemble in a precise manner to control cellular processes. (eurekalert.org)
  • endothelial
  • Presently, we describe the stimulating effect of B16F10-Nex2 melanoma cells on endothelial cells in a co-culture model of angiogenesis on Matrigel in vitro. (biomedcentral.com)
  • The first surface treatment layer possesses endothelial cell growth enhancing properties, while the second surface treatment layer includes the anti-proliferative or anti-angiogenic drug. (google.co.uk)
  • a surface treatment covering at least a portion of the thrombosis filter, wherein the surface treatment comprises two layers, a first surface treatment layer located between a second surface treatment layer and the portion of the thrombosis filter, the first surface treatment layer having properties which enhance endothelial cell growth, and the second surface treatment layer including an anti-proliferative or anti-angiogenic drug. (google.co.uk)
  • 5. A thrombosis filter element according to claim 1 , wherein the surface treatments incorporate, respectively, the anti-proliferative or anti-angiogenic drug and the endothelial cell growth enhancer with a bio-compatible polymeric material, or a combination of the surface treatment layers with or without the incorporation of the bio-compatible polymeric material. (google.co.uk)
  • Hypoxia also enhances the angiogenic potential of ES by inducing Y2Rs in endothelial cells and increasing the release of its ligand, NPY3-36, from ES cells. (nih.gov)
  • regulate
  • The surface treatment, comprised in part of an anti-proliferative or anti-angiogenic drug, is applied to portions of the thrombosis filter in order to regulate neointimal growth around the thrombosis filter and its anchor elements. (google.co.uk)
  • These observations suggest that connections must exist between the pathways that regulate cell growth and the cell cycle machinery. (mdpi.com)
  • membrane
  • We explain the observed cell volume changes by modeling flux of water and solutes across the cell membrane. (pnas.org)
  • To survive, cells rely on the passive response of mechanosensitive channels, which open under increased membrane tension and allow the release of cytoplasmic solutes and water. (pnas.org)
  • Protein levels of Y2Rs and DPPIV were detected in cell membrane fractions isolated from these cells by Western blot. (nih.gov)
  • D. DPPIV activity was measured in the above cell membrane fractions via luminescent method. (nih.gov)
  • Each circular DNA strand then attaches to the cell membrane , sometimes by a mesosome. (bionity.com)
  • The cell membrane then invaginates (grows inwards) and splits the cell into two daughter cells, separated by a newly grown cell plate. (bionity.com)
  • Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the plasma membrane (PM) to the nucleus, where they alter gene transcription. (novusbio.com)
  • biologically active
  • Water soluble macromers are modified by addition of free radical polymerizable groups, such as those containing a carbon-carbon double or triple bond, which can be polymerized under mild conditions to encapsulate tissues, cells, or biologically active materials. (google.es)
  • The polymeric materials are particularly useful as tissue adhesives, coatings for tissue lumens including blood vessels, coatings for cells such as islets of Langerhans, coatings, plugs, supports or substrates for contact with biological materials such as the body, and as drug delivery devices for biologically active molecules. (google.es)
  • secrete
  • The multipotent cells secrete a variety of soluble and insoluble molecules associated with stem cell niches in the body, as well as rapid tissue regeneration and scarless healing. (prweb.com)
  • stem
  • By growing cells under proprietary conditions that simulate the embryonic environment, particularly hypoxia, it has been shown that normal human fibroblast cells become multipotent, and express key stem cell markers including Oct4, Sox2 and Nanog. (prweb.com)
  • In previous work, Dr. Daniel Lacorazza, assistant professor of pathology at BCM, along with his research team, identified a transcription factor, ELF4, which regulates blood stem cells. (medindia.net)
  • A major obstacle to stem cell therapy and stem cell research is translating successful research into scalable, sustainable and affordable therapeutics. (merckmillipore.com)
  • Pharmaceutical and biomedical researchers working on the front lines of stem cell science and translational medicine need a partner with a proven track record of advancing cellular therapy product manufacturing: One with optimized bioproduction platforms, purification and characterization tools, and the process development services required to tackle the bottlenecks of autologous and allogeneic cell therapy commercialization. (merckmillipore.com)
  • Stem cells can now be grown and harvested from microcarriers adequately agitated in bioreactors with proven efficiency and reproducibility. (merckmillipore.com)
  • Stem cell manufacturers must also verify that the expanded and harvested cells maintain their critical quality attributes. (merckmillipore.com)
  • Flow cytometry systems are also used to analyze the quality and potency of stem cells. (merckmillipore.com)
  • The hypoxia-induced increase in DPPIV activity is most evident in a population of ES cells with high aldehyde dehydrogenase (ALDH) activity, rich in cancer stem cells (CSCs). (nih.gov)
  • The levels of ALDH-1 protein and stem cell marker, Oct-4, were compared by Western blot in soluble protein fractions of ALDHhigh and ALDHlow cells, to confirm the efficiency of cell sorting. (nih.gov)
  • Plant cloning is a simple process that allows you to replicate a plant by clipping a stem and replanting it. (wikihow.com)
  • metabolic
  • By identifying that this autophagy "addiction" is prevalent in cancers with Ras mutations, such as lung, pancreatic and colon, a metabolic vulnerability of cancer cells is revealed - a vulnerability the authors say can be utilized for cancer therapy. (healthcanal.com)
  • GO annotation shows that 2512 of the 2,636 differentially expressed genes (DEGs) are mostly distributed in metabolic process, cell and cell part, and binding, and KEGG annotation shows that 1,941 DEGs are mapped into 277 pathways. (biomedcentral.com)
  • densities
  • Therefore, only a small proportion of MTBs can be axenically isolated and cultured, and the obtainable cell densities do not allow the purification of sufficient numbers of magnetosomes for industrial applications ( Jogler and Schüler, 2009 ). (frontiersin.org)
  • fundamental
  • Here, we investigate the fundamental design principles of Escherichia coli 's passive mechanosensitive response to osmotic downshocks by implementing single-cell high-resolution imaging. (pnas.org)
  • stimulate
  • Canadian and Finnish researchers have identified a protein called Wnt4, which can stimulate the production of T-cells, the white blood cells that recognise and kill infectious agents. (medindia.net)
  • This truncated peptide no longer binds to Y1R and, therefore, does not stimulate ES cell death.Hypoxia-driven actions of the peptide such as these may contribute to ES progression.Due to the receptor-specific and multifaceted nature of NPY actions, these findings may inform novel therapeutic approaches to ES. (nih.gov)
  • This truncated peptide no longer binds to Y1R and, therefore, does not stimulate ES cell death. (nih.gov)
  • gene
  • These results suggest that HCF‐1 links the regulation of exit from mitosis and the G 1 phase of cell growth, possibly to coordinate the reactivation of gene expression after mitosis. (embopress.org)
  • In tsBN67 cells, the one X‐linked copy of the HCF‐1 gene carries a proline to serine substitution at position 134 (P134S) in the HCF‐1 Kelch domain. (embopress.org)
  • Through our SAFC BioReliance offering we provide world-class process development and manufacturing capabilities for virus based therapeutic products (e.g. gene therapy and viral vaccines). (merckmillipore.com)
  • Thimet oligopeptidase (TOP) and neurolysin activities were then investigated in B16F10-Nex2 melanoma cells aiming at gene sequencing, enzyme distribution and activity, influence on tumor development, substrate specificity, hydrolytic products and susceptibility to inhibitors. (biomedcentral.com)
  • tumors
  • In this new study, Dr. White and colleagues have found that cancer cells induce autophagy and this self-cannibalization process enables the growth of the most aggressive tumors. (healthcanal.com)
  • Finally, the hybrid model is used to study the acid-mediated growth of solid tumors. (rice.edu)
  • grown
  • By altering the conditions under which cells are grown, we can actually trigger the cells to revert back to an earlier, multipotent state. (prweb.com)
  • Histogen is a regenerative medicine company developing solutions based upon the products of cells grown under proprietary conditions that mimic the embryonic environment, including low oxygen and suspension. (prweb.com)
  • ligands
  • Our portfolio includes protein products (Protein A affinity ligands, cell culture growth factors), chromatography products (OPUS® pre-packed columns, chromatography resins, ELISA kits) and filtration products (XCell™ ATF systems, SIUS™ flat sheet TFF cassettes, and Spectrum KrosFlo™ hollow fiber TFF cartridges and systems). (globenewswire.com)
  • The Protein A ligands and growth factor products that we produce are key components of Protein A affinity resins and cell culture media, respectively. (globenewswire.com)
  • bioreactor
  • Our XCell™ ATF Systems, available in stainless steel and single-use configurations, continuously eliminate waste from a bioreactor to concentrate cells and significantly increase productivity in upstream processes. (globenewswire.com)
  • density
  • Maximal values of cell density (OD 565 ) (19.4) and cell yield (dry weight) (4.76 g/L) were attained at 40 h. (frontiersin.org)
  • At the key time point (18-20 h), cell density is high and magnetosomes have matured. (frontiersin.org)
  • Improved methods are needed to increase MTB cell density and magnetosome yield at a reasonable cost. (frontiersin.org)
  • bacteria
  • Memory T cells are created when naive CD8 T cells are activated to fight intracellular pathogens such as viruses or bacteria. (medindia.net)
  • Bacteria, like the majority of walled cells, live and grow under significant osmotic pressure. (pnas.org)
  • vaccines
  • Researchers say, identifying one of the processes that plays a role in naive and memory T-cells' growth and production can lead to better vaccines and possibly more effective cancer immunotherapy. (medindia.net)
  • populations
  • This approach allowed for testing two cell populations with maximal difference in ALDH activity. (nih.gov)
  • phenotype
  • Using mice generated to lack ELF4, researchers found that CD8 T-cells grew over time and acquired a "memory phenotype" without being exposed to any type of infections. (medindia.net)
  • We then examined the surface phenotype, cytokine profile, endocytic and T-cell-activation ability of these cells in support of the proteomic data, and obtained confirmatory evidence for differences in the maturation status and functional attributes between mDCs and the two DC models. (biomedcentral.com)
  • KG1 cells, which acquire a DC-like phenotype after stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin [ 6 ], are probably the most widely used in DC research. (biomedcentral.com)
  • vitro
  • We could enhance in vitro T cell activation of T cells extracted from patients to heighten immune response", said Lacorazza. (medindia.net)
  • maximal
  • At this point, cells entered the log phase of growth, the maximal values of C mag (1.78), iron content (0.47%), and magnetosome number (26 ± 3 per cell) were observed, superoxide dismutase (SOD) activity began to decrease more rapidly, ATP content dropped to an extremely low level (0.17 fmol), and reducing power (NADH/NAD + ratio) began to increase very rapidly. (frontiersin.org)
  • behavior
  • and (c) equations describing how cell behavior is modulated by the local concentration fields. (rice.edu)
  • regulator
  • While Target of Rapamycin (TOR) is the master regulator of growth, the cell cycle is dictated by the activity of the cyclin-dependent kinases (CDKs). (mdpi.com)
  • Receptors
  • Neuropeptide Y (NPY), and two of its receptors, Y1R and Y5R are up-regulated by EWS-FLI1 and abundantly expressed in ES cells. (nih.gov)
  • Akt1 acts as a transducer for growth factor receptors that modulate phosphatidylinositol 3-kinase (PI3K) activity. (novusbio.com)
  • regulatory
  • Other barriers are ensuring the long-term commercial viability of their cellular therapy products, developing scalable, robust, cost-effective cell culture systems, and ensuring that cGMP, documentation and regulatory requirements are strictly adhered to. (merckmillipore.com)
  • mitosis
  • Proteolytic processing is necessary to separate and ensure these two HCF‐1 activities, which are performed by separate HCF‐1 subunits: the HCF‐1 N subunit promotes passage through the G 1 phase whereas the HCF‐1 C subunit is involved in proper exit from mitosis. (embopress.org)
  • On the other hand, in fission yeast, cells need to reach a critical size to enter mitosis: a minimal size that can be modified in response to environmental changes [ 4 ]. (mdpi.com)
  • inhibitor
  • The non-stained cells or the cells incubated with Aldefluor in the presence of ALDH inhibitor, DEAB, served as negative controls. (nih.gov)
  • purification
  • Our innovative line of OPUS® chromatography columns, used in downstream processes for bench-scale to commercial-scale purification needs, are delivered pre-packed and customized to our customers choice of resin and packed bed height. (globenewswire.com)
  • arrest
  • We discovered that ELF4 directly activates the tumor suppressor KLF4, which signals cell cycle arrest in na ve CD8 T cells," Lacorazza said. (medindia.net)
  • line
  • In addition, the human multipotent cell conditioned media produced through Histogen's technology process can be found in the ReGenica line of skincare products by Suneva Medical. (prweb.com)
  • The use of nonphenolic linker did not improve retention of the radioactivity in A431 carcinoma cell line. (diva-portal.org)
  • researchers
  • Once illuminated, that step in the process can be exploited so that researchers can find ways to maximize the benefits of autophagy inhibitors and improve clinical outcomes," she stated. (healthcanal.com)
  • expression
  • We detected the expression of oligopeptidases in murine melanoma cells of high invasiveness. (biomedcentral.com)
  • In ES cells, hypoxia induces another NPY receptor, Y2R, and increases expression of dipeptidyl peptidase IV (DPPIV), an enzyme that cleaves NPY to a shorter form, NPY3-36. (nih.gov)
  • To assess expression of the NPY system in ES CSC, SK-ES1 cells were sorted by flow cytometry into ALDHhigh and ALDHlow cells (Supplementary Fig. 2). (nih.gov)
  • conditions
  • Our results indicate the existence of an optimal channel diameter for each set of cell parameters and culture conditions. (rice.edu)
  • The major obstacles involve the nutritional requirements and culture conditions of the cells. (frontiersin.org)
  • Culture under hypoxic conditions increased the number of SK-ES1 cells with elevated ALDH activity (Fig. 4A), suggesting hypoxia-driven enrichment in CSCs. (nih.gov)
  • Escherichia coli cells are able to divide every 20 minutes under optimum conditions. (bionity.com)