Cell growth inhibition k562 cells. Medical search. Frequent questions

Expression of MLL-fusions enhances proliferation and blocks myeloid differentiation of hematopoietic progenitor cells, leading to their pathological accumulation. (nature.com)
The compound does not inhibit the proliferation of normal fibroblasts nor that of hemopoietic progenitor cells. (tripod.com)
The regulated expression and degradation of proteins are critical to all aspects of cell development and proliferation. (biomedcentral.com)
However, c-Jun also plays a critical role in cell proliferation and apoptosis. (tmu.edu.tw)
In this study, we investigated the physiological roles of c-Jun in cell proliferation, apoptosis and erythroid differentiation of K562 cells. (tmu.edu.tw)
These clones have the same proliferation rate as the parental cell line in general culture medium. (tmu.edu.tw)
Our group found that the proliferation and differentiation ability of aged human bone marrow mesenchymal stem cells (hBM-MSCs) was decreased whereas cell senescence was increased [ 7 ]. (biomedcentral.com)
They also found that downregulation of miR10a/10b in clonal cells interfered with cell proliferation and enhanced cell apoptosis by activating the NF-κB-dependent p53 pathway [ 13 ]. (biomedcentral.com)
In myeloid leukemia cells, CaMKII, activated by the bcr-abl oncogene, promotes cell proliferation. (unisa.it)
Inhibition of CaMKII activity restricts cell proliferation, and correlates with growth arrest and differentiation. (unisa.it)
Conversely, expression of CaMKIV inhibits autophosphorylation and activation of CaMKII, and elicits G0/G1cell cycle arrest,impairing cell proliferation. (unisa.it)
Our data reveal a novel cross-talk between CaMKII and CaMKIV and suggest that CaMKII suppresses the expression of CaMKIV to promote leukemia cell proliferation. (unisa.it)
P21-activated kinases (PAKs) are serine/threonine kinases involved in the regulation of cell survival, proliferation, inhibition of apoptosis, and the regulation of cell morphology. (sciencegate.app)
Effects of sinusoidal magnetic field observed on cell proliferation, ion concentration, and osmolarity in two human cancer cell lines med. (emf-portal.org)
In previous research, the authors demonstrated that the methanol extract of Artemisia vulgaris (AVM) has the ability to inhibit chronic myeloid leukemia (CML) cell proliferation. (spandidos-publications.com)
Here, we determined the effect of Jiyuan oridonin A (JOA), an ent -kaurene diterpenoid compound, on the differentiation blockade in imatinib-sensitive, particularly, imatinib-resistant CML cells with BCR-ABL-T315I mutation by cell proliferation assay, apoptosis analysis, cell differentiation analysis, cell cycle analysis and colony formation assay. (jcancer.org)
We found that JOA at lower concentration significantly inhibited the proliferation of CML cells expressing mutant BCR-ABL (T315I mutation included) and wild-type BCR-ABL, which was due to that JOA induced the cell differentiation and the cell cycle arrest at G0/G1 phase. (jcancer.org)
BCR-ABL oncogene encodes a BCR-ABL tyrosine kinase (TK), which triggers different downstream targets including c-Myc, STAT5 and CrkL, which participated in the control of cell differentiation, proliferation, migration and other cellular behaviors [ 3 - 5 ]. (jcancer.org)
To evaluate the leukemogenic role of RUNX1-MT in ASXL1 -mutated cells, we co-expressed RUNX1 -MT (R135T) and ASXL1 -MT (R693X) in different cell lines and performed immunoblot, co-immunoprecipitation, gene expression microarray, quantitative RT-PCR, cell proliferation, differentiation, and clonogenic assays for in vitro functional analyses. (biomedcentral.com)
The expression of RUNX1 mutant in ASXL1 -mutated myeloid cells augmented proliferation, blocked differentiation, and increased self-renewal activity. (biomedcentral.com)
Bosutinib is a potent inhibitor of CML cell proliferation in vitro and has demonstrated promising activity in CML patients resistant or intolerant to IM as well as in newly diagnosed patients with chronic phase CML (CML-CP). (mdm2-receptor.com)
The inhibitory effects of bufalin on cell proliferation were determined by MTT (Methyl thiazolyltetrazolium) assay. (biomedcentral.com)
1) Bufalin inhibited the proliferation of SW620 cells. (biomedcentral.com)

Induction of apoptosis in human leukaemic cell lines K562, HL60 and U937 by diethylhexylphthalate isolated from Aloe vera Linne. (tripod.com)

Many factors and components play a role in the cell cycle of K562 cells in terms of growth, cell differentiation, and apoptosis. (wikipedia.org)
The growth of these leukemic cells are controlled by either initiating cell differentiation or apoptosis to occur. (wikipedia.org)
Cell differentiation is induced by the deacetylase activity in these "undifferentiated progenitor cells," which alters the phenotype and morphology of the K562 cells. (wikipedia.org)
This gene targets the cyclin-dependent kinase inhibitor, p21, and causes cell differentiation, cell cycle arrest in G1, and ultimately apoptosis. (wikipedia.org)
Other methods being focused on in the regulation of K562 cells include therapeutic methods like polyphyllin D, which caused differentiation from the progenitor state to occur, and for apoptosis to begin. (wikipedia.org)
SETD2 loss causes growth arrest and differentiation of AML cells, and leads to increased DNA damage. (nature.com)
SETD2 loss synergizes with pharmacologic inhibition of the H3K79 methyltransferase DOT1L to induce DNA damage, growth arrest, differentiation, and apoptosis. (nature.com)
c-Jun did not alter growth inhibition and apoptotic induction by STI571 treatment, but inhibited STI571-induced erythroid differentiation. (tmu.edu.tw)
Moreover, c-Jun did not alter growth inhibition and apoptotic induction by histone deacetylase (HDAC) inhibitors (apicidin, sodium butyrate, and MS275) treatment, but inhibited HDAC inhibitors-induced erythroid differentiation. (tmu.edu.tw)
These results suggest that c-Jun may modulate anticancer drugs-induced cell differentiation but not growth inhibition and apoptosis in CML cells. (tmu.edu.tw)
Huang, HM & Liu, JC 2009, ' c-Jun blocks cell differentiation but not growth inhibition or apoptosis of chronic myelogenous leukemia cells induced by STI571 and by histone deacetylase inhibitors ', Journal of Cellular Physiology , vol. 218, no. 3, pp. 568-574. (tmu.edu.tw)
We demonstrated previously that miR-10a is significantly decreased in aged hBM-MSCs and restoration of the miR-10a level attenuated cell senescence and increased the differentiation capacity of aged hBM-MSCs by repressing Krüpple-like factor 4 (KLF4). (biomedcentral.com)
However, other studies have suggested that miR-10a may have additional effects other than regulating cell senescence and differentiation. (biomedcentral.com)
The mechanism by which the inhibition of CaMKII results in growth arrest and differentiation in myeloid leukemia cells is still unknown. (unisa.it)
Chronic myeloid leukemia (CML) results from BCR-ABL oncogene, which blocks CML cells differentiation and protects these cells from apoptosis. (jcancer.org)
Mechanistically, the cell differentiation mediated by JOA may be originated from the inhibition of BCR-ABL/c-MYC signaling in CML cells expressing wild-type BCR-ABL and BCR-ABL-T315I. (jcancer.org)
JOA displayed the activity of inhibiting the BCR-ABL and promoted differentiation of not only imatinib -sensitive but also imatinib -resistant cells with BCR-ABL mutation, which could become a potent lead compound to overcome the imatinib -resistant induced by inhibitors of BCR-ABL tyrosine kinase in CML therapy. (jcancer.org)

Also zinc nanoparticles significantly (p=0.021) inhibit K562 cell line. (ac.ir)
Based on this finding it should be concluded that combination of zinc nanoparticles with Saccharomyces cell walls could inhibit the growth of K562 cell line in vitro . (ac.ir)
K562 cells can spontaneously develop characteristics similar to early-stage erythrocytes, granulocytes and monocytes and are easily killed by natural killer cells as they lack the MHC complex required to inhibit NK activity. (wikipedia.org)
When the levels of these components are thrown off, they can either no longer inhibit apoptosis of the cancer cells, a role fulfilled by BCR/ABL, or they cause apoptosis to be induced, in the same vein as Bax and cytochrome C. These components are key in the mitochondria, and due to this, it has been supported that apoptosis uses the mitochondrial apoptosis pathway. (wikipedia.org)
These potent effects are driven by curcumin's ability to induce G2/M cell cycle arrest, induce autophagy, activate apoptosis, disrupt molecular signaling, inhibit invasion and metastasis, and increase the efficacy of current chemotherapeutics. (hindawi.com)
These mice are devoid of endogenous natural killer and T-cell activity and were used to determine whether adoptively transferred expanded natural killer cells could inhibit myeloma growth and myeloma-associated bone destruction. (haematologica.org)
STI571 could inhibit c-Jun expression in K562 cells, but not in c-Jun-overexpression cells. (tmu.edu.tw)
In previous research, the authors demonstrated that the methanol extract of A. vulgaris (AVM) has the ability to inhibit the viability of CML cells ( 10 ). (spandidos-publications.com)
This is supported by pharmacological targeting where the first-generation pan-BET bromodomain inhibitor JQ1(+) displays anti-inflammatory effects and inhibit tumor cell eradication, while the novel bivalent BET bromodomain inhibitor AZD5153, which shows differential activity towards BET family members, does not. (frontiersin.org)

Cell-based and in vitro assays show that Retro-2 blocks delivery of newly-synthesized TA-proteins to the ER-targeting factor ASNA1 (TRC40). (elifesciences.org)
We tested whether natural killer cells expanded by co-culture with K562 cells transfected with 41BBL and membrane-bound interleukin-15 could kill myeloma cells with a high-risk gene expression profile in vitro and in a unique model which recapitulates human myeloma. (haematologica.org)
In vitro experiments further confirmed knockdown of TRAIP inhibited cell migration and invasion, as well as decreasing chemokine production and inhibiting M2-like macrophage recruitment. (bvsalud.org)
The hypoxia-induced apoptosis, cell survival, and cell paracrine function of aged hBM-MSCs were investigated in vitro. (biomedcentral.com)
Through lentivirus-mediated upregulation of miR-10a and downregulation of KLF4 in aged hBM-MSCs in vitro, we revealed that miR-10a decreased hypoxia-induced cell apoptosis and increased cell survival of aged hBM-MSCs by repressing the KLF4-BAX/BCL2 pathway. (biomedcentral.com)
in vitro cytotoxic effects of GCV to these cells demonstrated that concentrations of about 2.5 μM for K562/LTRNL and 1.25μM for MEG-01/LTRNL cells resulted in 50% inhibition of cell growth after 72 hr. (elsevierpure.com)
MTT assay was used to investigate the effect of IVM on cancer cells growth in vitro . (alecomm.com)
Our results indicated that ivermectin at its very low dose, which did not induce obvious cytotoxicity, drastically reversed the resistance of the tumor cells to the chemotherapeutic drugs both in vitro and in vivo . (alecomm.com)
In vitro tests showed that after incubation with NSC631570 normal liver cells and Ehrilch tumour ascitic cells demonstrate different oxygen consumption: after initial increase, oxygen consumption in cancer cells falls to zero whereas oxygen consumption in normal cells returns to normal and the cells remain undamaged (38). (ukrin.com)

In the last years, adoptive transfer of natural killer (NK) cells came into the focus of translational medicine, because of their high cytotoxic potential against transformed malignant cells. (frontiersin.org)
Hence, we established a two-phase expansion protocol with IL-15 to induce an early NK cell expansion, followed by short exposure to IL-21 that boosted the cytotoxic activity of NK cells against RMS cells. (frontiersin.org)
Doxorubicin conjugated micelles with HER-2 peptide (DOX-HER-2-NCs) had more cytotoxic effects on HER-2 positive cells. (iyte.edu.tr)

Bruceine D exhibited potent cytotoxicity to K562 cells with IC50 of 6.37 ± 0.39 μM. (edu.hk)
The apoptosis rate was increased on SKBR-3 at 50% cell growth inhibition (IC50) as determined by Annexin-V/Propidium iodide double staining. (iyte.edu.tr)
Additionally, DOX-HER-2-NCs resulted in cell cycle arrest at G2/M-phase in response to IC50 value. (iyte.edu.tr)

Inhibition of DNA-PKcs by NU7026 markedly potentiated the apoptotic and growth inhibitory effects of hydroquinone in proerythroid leukemic K562 cells in a dose-dependent manner. (cdc.gov)
CSN5 knockdown caused mitotic defects, G2/M arrest and apoptotic cell death. (biomedcentral.com)
CSN2 knockdown resulted in non-apoptotic cell death associated with accumulation of both the autophagy marker LC3-II and autophagic vacuoles. (biomedcentral.com)
In the cell morphology analyzes, chromatin condensation and marginalization, changes in the cell membrane, ghost cells and apoptotic bodies were observed as evidence of apoptosis formation. (ijpsonline.com)
However, the applicability of such traditional chemotherapeutic approaches is primarily limited due to acquired Multi-Drug Resistance (MDR) in cells, which is acquired through the production of anti-apoptotic cascades and the overexpression of cell membrane-based drug efflux pumps, such as P-glycoprotein, for self-defense. (ijpsonline.com)
The nuclei of apoptotic and necrosis cells were observed using fluorescence microscopy [13]. (egfrsignals.com)

Expanded natural killer cells killed both allogeneic and autologous primary myeloma cells avidly via a perforin-mediated mechanism in which the activating receptor NKG2D, natural cytotoxicity receptors, and DNAX-accessory molecule-1 played a central role. (haematologica.org)
Extracts of Aloe vera Linne have been found to exhibit cytotoxicity against human tumour cell lines. (tripod.com)
The cytotoxicity mechanism consists of the induction of apoptosis, whereas the selectivity against neuroectodermal tumor cells is founded on a specific energy-dependent pathway of drug incorporation. (tripod.com)

Chronic myeloid leukemia is a common cancer in human, so the goal of this study was the use of natural compound such as cell wall obtained from Saccharomyces cerevisiae (S. cerevisiae) and Saccharomyces boulardi (S. boulardi) and zinc nanoparticles on the growth inhibition of K562 cell line. (ac.ir)
Chronic myeloid leukemia (CML), an acquired malignant myeloproliferative disorder of hematopoietic stem cells, is one of the three most common forms of leukemia. (edu.hk)
on human chronic myeloid leukemia K562 cells. (edu.hk)
Greater than 95% knockdown of the non-catalytic subunit CSN2 and the deneddylating subunit CSN5 of the CSN was achieved in the human myeloid progenitor cell line K562. (biomedcentral.com)
The relationship between CaMKII and CaMKIV is also observed in primary acute myeloid leukemia (AML) cells, and it correlates with their immunophenotypic profile. (unisa.it)
In this study, therapeutic effects of drug-conjugated nanocarrier system with enhanced stability and double moiety pH-sensitivity on breast cancer (SKBR-3- HER-2- positive), normal breast epithelial (MCF-10A, HER-2-negative) and chronic myeloid leukemia (K562, HER-2-negative) cells were determined. (iyte.edu.tr)
Holyoake, T.L. A comparison of normal and leukemic stem cell biology in chronic myeloid leukemia. (eurekaselect.com)
Chronic myeloid leukemia (CML) is a clonal expansion of the progenitor hematopoietic stem cells arising from the existence of the fusion BCR-ABL oncogene, that represents 15%-20% of the newly diagnosed cases of leukemia patients [ 1 - 2 ]. (jcancer.org)
Co-expression of two mutant genes increased myeloid stem cells in animal model, suggesting that cooperation of RUNX1 and ASXL1 mutations played a critical role in leukemia transformation. (biomedcentral.com)
Hematological malignancies, which include lymphoid, myeloid, histiocytic, and mast cell neoplasms, are a heterogeneous group of diseases of diverse incidence, pathogenesis and prognosis (Kornblau et al . (bbrc.in)

Treatment of vector control K562 cells with the autophagy inhibitors 3-methyladenine and bafilomycin A1 recapitulated the growth kinetics, vacuolar morphology and LC3-II accumulation of CSN2 knockdown cells indicating that the cellular phenotype of CSN2 cells arises from autophagy inhibition. (biomedcentral.com)
Apoptosis induction potential of each compound determined by mitochondrial membrane potential analysis (Rho123), cleaved caspase-3 expression analysis by flow cytometry and immunofluorescent staining and cell morphology analysis by giemza, hematoxylin and eosin and Papanicolaou protocols. (ijpsonline.com)

Tumor cell resistance to anthracyclines and epipodophyllotoxins can be due to reduced drug accumulation and/or alterations in the activity of topoisomerase II (TOPO II). (ucy.ac.cy)
In addition, hyperpolarized tumor cell membranes and mitochondrial membrane potentially allow selective accumulation of mitochondria-targeted drugs. (biomed.news)
In contrast, knockdown of BRD4 but not of BRD2 impairs NK cell cytolytic responses, suggesting BRD4 as critical regulator of NK cell mediated tumor cell elimination. (frontiersin.org)

It led to loss of Δψm, release of cytochrome c, activation of caspases-9, -3 and cleavage of PARP, which suggested that bruceine D induced apoptosis of K562 cells through mitochondrial pathway. (edu.hk)
We demonstrated that inhibition of the IL-6 signaling pathway by MR16-1 treatment resulted in significant recovery of iron-deficiency anemia and alleviation of cancer-related symptoms. (biomedcentral.com)
The Part 1 post in this series is concerned principally with anti-cancer interventions that address the mTOR pathway, a growth pathway also of great interest from the viewpoint of longevity. (anti-agingfirewalls.com)
The purpose of the research is to investigate the roles of Jak-STAT3 signaling pathway in bufalin-induced apoptosis in colon cancer SW620 cells. (biomedcentral.com)
Activation of caspase-3, up-regulation of Bax, down-regulation of livin and Bcl-2, as well as inhibition of Jak-stat3 signaling pathway might be the important mechanisms for the bufalin-induced apoptosis. (biomedcentral.com)

In line, many MLL-fusions can act as potent oncogenes in cell line models and animal models of leukemia 4 . (nature.com)
These results indicate that DEHP isolated from Aloe vera Linne has a potent antileukaemic effect, and thus represents a new type of pharmacological activity with respect to human leukaemic cells. (tripod.com)

Researchers at the University of Natural Resources and Life Sciences, Vienna compared NSC631570's inhibitive effect on the reproduction of malignant and normal cells. (ukrin.com)

MTT assay was used to evaluate cell growth inhibition. (edu.hk)
The B16F10 cell line viability after treatment with increasing concentrations of different fractions of the plant (5-60 µg/mL) was measured using MTT assay. (ac.ir)
At a level of 10 microg mL(-1) DEHP a significant anti-leukaemic effect was observed for all three cell lines, as measured by clonogenic assay. (tripod.com)
Results C. butyricum stimulates elevated levels of IL-10 in HT-29 cells To investigate whether C. butyricum regulates IL-10 expression in HT-29 cells, a stimulation assay was performed, as described in the methods. (egfrsignals.com)
The same cells from the culture media were collected, and subjected to real-time PCR assay. (egfrsignals.com)

Inhibiting these is an important regulation mechanism of cancer, because it prevents cells from progressing into mitosis. (wikipedia.org)
Apoptosis is an important mechanism in regulating K562 cells and can be induced by the changes in the metabolic state of the cells. (wikipedia.org)
If the greater 17-DMAG-induced cell death in Atm_/_ cells might possibly be attributed to an enhanced accumulation of p53 and Nilotinib the mechanism by way of which this might come about remain unclear. (cd31-signal.com)
No matter the underlying mechanism via which the loss of Atm renders cells extra delicate to 17-DMAG, importantly for our research, 17-DMAG-induced cell death clearly proceeds in the absence of Atm. (cd31-signal.com)
The figures indicate that for each the siRNA-treated and -control cells, the interaction line lies beneath the no-interaction line indicating mechanism-based tsa inhibitor synergy. (cd31-signal.com)

Furthermore, U937 cells expressing CaMKIV show elevated levels of Cdk inhibitors p27(kip1) and p16(ink4a) and reduced levels of cyclins A, B1 and D1. (unisa.it)
The overall goals of this review are to describe the roles for PAK kinases in cell signaling and disease, and to describe how the use of nanomedicine is a promising new method for administering PAK inhibitors for the purpose of disease treatment and research. (sciencegate.app)

We investigated the effect of diethylhexylphthalate (DEHP) from Aloe vera Linne on the apoptosis of human leukaemic cell lines K562, HL60 and U937 to examine its pharmacological activity. (tripod.com)
At 100 microg mL(-1) DEHP, K562, HL60 and U937 leukaemic cell lines showed growth inhibition of 95, 97 and 95%, respectively. (tripod.com)
DEHP exhibited an inhibitory activity of 74, 83 and 81%, respectively, in K562, HL60 and U937 cell lines at a concentration of 10 microg mL(-1). (tripod.com)
At a concentration of 1 microg mL(-1), DEHP exerted an inhibitory activity of 50, 51 and 52%, respectively, in K562, HL60 and U937. (tripod.com)

The ability to induce these changes in K562 cell cycle and cell cycle regulation provides targets for cancer drugs. (wikipedia.org)

These play a role in cellular stress, metabolism, and autophagy, by interacting with deacetylases activity in the cell. (wikipedia.org)
Frequently, low doses of natural chemical products activate an adaptive stress response, whereas high doses activate acute responses like autophagy and cell death. (hindawi.com)

The results showed that S. boulardi cell wall significantly (p=0.029) inhibits the growth of K562 cell line compared to S. cerevisiae . (ac.ir)
K562 cells were the first human immortalised myelogenous leukemia cell line to be established. (wikipedia.org)
K562 cells are of the erythroleukemia type, and the cell line is derived from a 53-year-old female chronic myelogenous leukemia patient in blast crisis. (wikipedia.org)
K562 cells are part of the NCI-60 cancer cell line panel used by the National Cancer Institute. (wikipedia.org)
Another important group of regulators of the K562 line are Sirtuins, referred to as SIRTS. (wikipedia.org)
The Leukemia-Lymphoma Cell Line Factsbook. (wikipedia.org)
The aim of this study was to evaluate the effect of methanol (MeOH), n -hexane and dichloromethane (CH 2 Cl 2 ) fractions of the extract on melanin synthesis and oxidative stress in B16F10 melanoma cell line. (ac.ir)
In a normal cell line, MDBK, DEHP exerted 30% growth inhibition at a concentration of 100 microg mL(-1), and showed no inhibitory activity at concentrations below 50 microg mL(-1). (tripod.com)
Recent studies reported that c-Jun inhibited hemoglobin synthesis in human CML cell line K562. (tmu.edu.tw)
In this study, anticancer properties of heterocyclic salicylaldimines and their Cu(II) complexes on K562 human chronic myelogenous leukemia cell line were investigated. (ijpsonline.com)
These findings were also confirmed in the K562 leukemic cell line. (unisa.it)
We defined the stem cell profile of K562 line, demonstrating the expression of the Embryonic Transcription Factors Oct3/4, Sox2, Klf4 and Nanog. (oncotarget.com)
With their ability to detect and directly destroy virally infected or malignant cells, natural killer (NK) cells form an important part of the first line defense of the immune system. (frontiersin.org)
They can be activated rapidly via germ-line encoded receptors that recognize the presence of stress ligands or absence of self-antigens on target cells ( 1 - 5 ). (frontiersin.org)
Nude mice were subcutaneously inoculated with cells of the IL-6-producing human lung cancer cell line LC-06-JCK and assessed as a model of cancer-related anemia. (biomedcentral.com)
In order to achieve 50% growth inhibition a tenfold concentration of NSC 631570 had to be administered to normal endothelial cells in comparison to a human osteosarcoma cell line. (ukrin.com)
Yet, for siRNAtreated cells, the interaction lies nearer on the no-interaction line indicating significantly less powerful synergy as also indicated by the interaction parameter worth of 0.413 when compared with 0.243 for your siRNA-control cells. (cd31-signal.com)

Inhibition of DNA-dependent protein kinase catalytic subunit by small molecule inhibitor NU7026 sensitizes human leukemic K562 cells to benzene metabolite-induced apoptosis. (cdc.gov)
The transferred, expanded natural killer cells proliferated in vivo in an interleukin-2 dose-dependent fashion, persisted up to 4 weeks, were readily detectable in the human bone, inhibited myeloma growth and protected bone from myeloma-induced osteolysis. (haematologica.org)
This study examines the anti-tumour effects of di(2-ethylhexyl)phthalate (DEHP) isolated from Aloe vera Linne, in human and animal cell lines. (tripod.com)
The growth of human neuroectodermal tumors is inhibited in mice with severe combined immunodeficiency without any appreciable toxic effects on the animals. (tripod.com)
Although studied in model human cell lines such as HeLa, very little is known about the role of the CSN in haemopoietic cells. (biomedcentral.com)
Aging is one of the key factors that regulate the function of human bone marrow mesenchymal stem cells (hBM-MSCs) and related changes in microRNA (miRNA) expression. (biomedcentral.com)
To identify determinants of mETC function, we screened a genome-wide human CRISPRi library under oxidative metabolic conditions with selective inhibition of mitochondrial Complex III and identified OCIA domain-containing protein 1 (OCIAD1) as a Complex III assembly factor. (biorxiv.org)
Cells of the human leukemia lines (K562, MEG-01) were infected with this vector and two transduced cell lines (K562/LTRNL, MEG-01/LTRNL) were established. (elsevierpure.com)
This study indicates that the LTRNL-infected human leukemia cells exhibit inducible susceptibility to GCV. (elsevierpure.com)
As a result, cells expressing different forms of BCR/ABL were recruited for the present study, including K562 (human wild‑type) or TCCY‑T315I (human imatinib‑resistant) and the Ba/F3‑(T315I/E279K/Y253H) (mouse BCR/ABL point mutation‑transfected cells). (spandidos-publications.com)
Thus, cells expressing different forms of BCR/ABL were recruited for the present study, including K562 [human wild-type (WT)] or TCCY-T315I [human imatinib-resistant (IR)] and the Ba/F3-(T315I/E279K/Y253H) (mouse BCR/ABL point mutation-tranfected cells). (spandidos-publications.com)
The hybrid virus could infect human airway cells and caused disease in mice, according to the team's results, which were published in Nature Medicine . (alecomm.com)
The results demonstrate the ability of the SHC014 surface protein to bind and infect human cells, validating concerns that this virus-or other coronaviruses found in bat species-may be capable of making the leap to people without first evolving in an intermediate host, Nature reported. (alecomm.com)
In the NCI test model, in contrast to conventional cytostatic preparations which caused the growth inhibition only in some cancer cell lines, like thiotepa inhibited the growth of MLI-09 (non-small lung cancer) and UOK-57LN (renal cancer), NSC631570 killed all 60 tested cancer cell lines (190), which represent the eight important human tumours, including the cell lines which were resistant to the strongest cytostatic drug at the time, cisplatin. (ukrin.com)
Tests on human cervical carcinoma cells HeLa, squamous cell carcinoma WHCO5 and normal equine lung cell lines demonstrated that NSC631570 is selectively toxic against cancer cells. (ukrin.com)
Vero cell vaccines were reported more commonly from respondents in Eastern Europe, Asia, and Africa, in contrast to clinics in North America and Western Europe, which primarily reported using human diploid cell and purified chick embryo cell vaccines. (c-kitinhibitors.com)

1 Significant advances have been made by combining novel agents with autologous peripheral blood stem cell transplantation which allows for long-term disease-free survival in the majority of transplant-eligible patients. (haematologica.org)
The mouse cardiac function of cardiac angiogenesis was measured and cell survival of aged hBM-MSCs was investigated. (biomedcentral.com)
In vivo, transplantation of miR-10a-overexpressed aged hBM-MSCs promoted implanted stem cell survival and improved cardiac function after MI. (biomedcentral.com)
In vivo development and survival of NK cells require cytokines ( 6 - 8 ). (frontiersin.org)
Mitochondria is the central site of cellular energy production and plays an important role in cell survival and death. (biomed.news)
Disruption of innate defense responses by endoglycosidase HPSE promotes cell survival. (uu.se)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Binding mode and structural elements of Bcr-Abl inhibition are discussed with emphasis on pathways involved in this complex disease to determine alternative strategies and combination therapies. (eurekaselect.com)
The Part 2 post in this series is concerned with mainline anti-cancer drug interventions that simultaneously address multiple growth pathways, ones that are entering clinical practice. (anti-agingfirewalls.com)
Following activation, NK cells mediate killing of target cells through two major pathways that require direct contact between NK cells and their target cells ( 6 ). (frontiersin.org)

This approach was based on the assumption that the deregulation of a protein was causally linked to a disease state, and the pharmacological intervention through inhibition of the deregulated target was able to restore normal cell function. (biomedcentral.com)

Flow cytometry revealed that the inhibition of this chain by antimycin A produced cell accumulation in the S phase of cell cycle and apoptosis. (oncotarget.com)
The cell cycle arrest and apoptosis were tested by flow cytometry analysis. (biomedcentral.com)

Inhibition of iduronic acid biosynthesis by ebselen reduces glycosaminoglycan accumulation in mucopolysaccharidosis type I fibroblasts. (uu.se)

One of these drugs is Imatinib, which inhibits BCR/ABL causing growth to cease and apoptosis to begin. (wikipedia.org)

This arrest leads to "shrinkage, blebbing, nuclear fragmentation, chromatin condensing" and other morphological changes that cause the cell to program death at this point. (wikipedia.org)
If this had been the situation, the broken Atm_/_ cells would fail to engage G1 arrest and progress by means of S-phase, major alternatively to Atr-dependent stalled replication forks and an increase in p53-dependent apoptosis in lieu of DNA repair. (cd31-signal.com)

Western blots show that STI571 inhibited c-Jun expression in a dose-dependent manner, reaching a maximum inhibition at 1 μM. (tmu.edu.tw)

AbstractRapid bacterial growth depends on the speed at which ribosomes can translate mRNA into proteins. (sciencegate.app)
Using a chemo-genetic approach, we identify BET bromodomain containing proteins BRD2 and BRD4 as central regulators of NK cell functions, including direct cytokine secretion, NK cell contact-dependent inflammatory cytokine secretion from monocytes as well as NK cell cytolytic functions. (frontiersin.org)
Recently, it has been reported by many workers that the potential functions of exosomes contributed to various aspects of hematological tumorigenesis, particularly with a focus on the exosome-mediated tumor progression, metastasis, drug resistance and immune escape by altering the function of receiver cells via diverse exosomal cargoes including proteins, DNA, messenger RNAs (mRNAs), and microRNAs) (Whiteside and Boyiadzis, (2017). (bbrc.in)

The effects of WR-1065 (2-((aminopropyl)amino)ethanethiol) on cell cycle progression, topoisomerase (topo) IIα activity, and topo IIα phosphorylation in Chinese hamster ovary (CHO) cells have been investigated. (ucy.ac.cy)

Anti - cancer property of different concentrations of the yeasts' cell wall with zinc nanoparticles were assayed by MTT and electrophoresis methods. (ac.ir)
The data showed that at concentrations below 60 µg/mL, fractions did not show significant toxicity on melanoma cells. (ac.ir)
Growth inhibition was specifically exerted by DEHP against three leukaemic cell lines at concentrations below 100 microg mL(-1). (tripod.com)
Figure 1A shows that IL-10 concentrations in the media of HT-29 cells cultured with C. butyricum were increased significantly. (egfrsignals.com)
This paradox might have significant impact on therapy with regard to the effects of TDX during the intervals of administration, when the drug concentrations become so low as to promote maintenance of dormant cancer cells in hypoxic tissue niches. (oncotarget.com)
At lower NSC631570 concentrations malignant cells die as a result of apoptosis, at higher concentrations the formation of microtubules is prevented und polyploidy occurs (62). (ukrin.com)

Previous studies proved that bone marrow (BM) stem cells play an important role in improving heart function and delaying cardiac remodeling [ 1 , 2 ] after ischemic damage through reducing fibrosis and increasing angiogenesis. (biomedcentral.com)

and Popov ex Poljakov on K562 and HL-60 cell lines. (ac.ir)
Firstly, we generated K562 cell lines stably overexpressing c-Jun. (tmu.edu.tw)
We report that inhibition of CaMKII activity results in an upregulation of CaMKIV mRNA and protein in leukemia cell lines. (unisa.it)
Seven compounds inhibited the growth of some cancer cell lines, whereas one of the 2-quinoline derivatives displayed favorable activity against all tested cancer cells with GI 50 values of 0.92-13 μM. (springer.com)

The change in phenotype induces a decrease in the growth rate and leads the K562 cells to the terminal path of becoming mature erythroids, monocytes, and mature macrophages. (wikipedia.org)
We conclude that CSN2 is required for CSN integrity and the stability of individual CSN subunits, and postulate that CSN2 loss results in a phenotype distinct from that of cells lacking CSN5 possibly as a consequence of altered CSN5 activity within a resultant CSN subcomplex. (biomedcentral.com)

The age of stem cell donors might significantly impact the recipient's endogenous responses [ 6 ]. (biomedcentral.com)
13 ] found that miR-10a and miR-10b were significantly increased in CD34 + marrow cells from 28 patients with myelodysplastic syndrome compared with healthy donors. (biomedcentral.com)
Drugs targeting diseased tissues or cells can significantly improve the efficacy of drugs. (biomed.news)

Leukemia cells in patients are intrinsically resistant to standard chemotherapy, and resistance development in leukemia cells toward drugs results in treatment failure[ 4 , 5 ]. (ijpsonline.com)
In summary, this two-phased feeder cell-free ex vivo culturing protocol combined efficient expansion and high cytolytic functionality of NK cells for treatment of radiation-resistant RMS. (frontiersin.org)
Interestingly, AVM appeared to be more sensitive to imatinib‑resistant (T315I, Y253H, and E279K) than wild‑type BCR/ABL cells, indicating its potential to overcome imatinib‑resistant severe issues in CML. (spandidos-publications.com)

Mitochondrial membrane potential was found at low levels in cells treated with Schiff base compounds and doxorubicin. (ijpsonline.com)

The Bcr-Abl protein induces the upregulation of proto-oncogene c-Jun, which is involved in Bcr-Abl transforming activity in Bcr-Abl positive cells. (tmu.edu.tw)

The cells are non-adherent and rounded, are positive for the bcr:abl fusion gene, and bear some proteomic resemblance to both undifferentiated granulocytes and erythrocytes. (wikipedia.org)
There are many different cellular components involved in the cycle of apoptosis such as BCR/ABL, Bcl-2, Bax protein, and cytochrome C. The tumor suppressor gene p53 is also important in the cell cycle regulation of K562 cells. (wikipedia.org)
Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated that TRAIP-associated genes were mainly involved in DNA replication, cell cycle and other processes. (bvsalud.org)
To investigate the possibility of killing tumor cells by the expression of an exogenously introduced toxic gene, we have constructed a novel retroviral vector (LTRNL) which has the polyA signal deleted herpes simplex virus type 1 thymidine kinase (HSV1-tk) gene. (elsevierpure.com)
GRP75-driven, cell-cycle-dependent macropinocytosis of Tat/pDNA-Ca2+ nanoparticles underlies distinct gene therapy effect in ovarian cancer. (uu.se)
Moreover, the RUNX1 mutant protein was more stable than WT and increased HIF1-α and its target ID1 gene expression in ASXL1 mutant cells. (biomedcentral.com)

Cancer cells are characterised by an elevated metabolic plasticity and enhanced production of reactive oxygen species (ROS), two features acknowledged as hallmarks in cancer, with a high translational potential to the therapeutic setting. (biomed.news)

New therapeutic concepts in anti-tumor therapy aim to modulate the patient's immune system to increase its aggressiveness or targeted effects toward tumor cells. (frontiersin.org)
In a proof of concept in vivo study, we also observed a therapeutic effect of adoptively transferred IL-15 expanded and IL-21 boosted NK cells in combination with image guided high precision radiation therapy using a luciferase-transduced RMS xenograft model. (frontiersin.org)
In this study, we sought to explore whether ivermectin (IVM), a macrolide antiparasitic agent, could overcome the resistance of cancer cells to the therapeutic drugs. (alecomm.com)

However, another study suggests that bcr:abl over-expression may actually increase cell adherence to cell culture plastic. (wikipedia.org)
The immune infiltration analysis indicated that TRAIP expression was tightly correlated with the infiltration of diverse immune cell types, including B cell, CD8 + T cell, neutrophil and dendritic cell. (bvsalud.org)
Overall we show that NOX2, through the regulation of NOX4 expression, controls metabolism and mitochondrial function in CML cells. (biomed.news)
Mechanistically, ivermectin reversed the resistance mainly by reducing the expression of P-glycoprotein (P-gp) via inhibiting the epidermal growth factor receptor (EGFR), not by directly inhibiting P-gp activity. (alecomm.com)
Re-expression of glucuronyl C5-epimerase in the mutant MEF cells increases heparan sulfate epimerization but has no influence on the Golgi localization and enzymatic activity of 2-O-sulfotransferase. (uu.se)
Mice transduced with both ASXL1 and RUNX1 mutations enhanced inhibitor of DNA binding 1 (ID1) expression in the spleen, liver, and bone marrow cells. (biomedcentral.com)
Stabilization of quadruplex architecture by small molecules is thus emerging as a potential anticancer approach since it is thought to interfere with oncogenic expression and telomeric maintenance in cancer cells [ 11 - 13 ]. (hindawi.com)

These changes can also drive the leukemic cells to a state of stress, which allows for increased sensitivity of the cells to drugs that initiate apoptosis. (wikipedia.org)

But these anti-cancer properties would warrant further study on the clinical application of yeast cell wall. (ac.ir)
The problem with K562 cells, and many other cancer cell types, is an overabundance of Aurora kinases. (wikipedia.org)
Leukemia is a blood cancer that develops in the hematopoietic system and presents in the bone marrow with or without peripheral blood cell involvement[ 1 , 2 ]. (ijpsonline.com)
This study brought the first indications that, in contrast to its starting substances thiotepa (a well known cytostatic agent) and greater celandine alkaloids, NSC631570 is in fact only toxic against cancer cells and not against normal cells. (ukrin.com)
The preparation has shown that the problem can be solved and has changed our ideas about healthy and cancer cells. (ukrin.com)
This means that NSC631570 can differentiate between healthy and malignant cells, a feature not yet managed by other anti-cancer drugs. (ukrin.com)
Numerous studies currently suggest that quadruplex formation in this overhang alters the structure and function of telomeres, inducing a damage response and rapid apoptosis in particular in cancer cells [ 14 - 24 ]. (hindawi.com)
Bufalin not only inhibited the growth of colon cancer SW620 cells, but also induced apoptosis of SW620 cells. (biomedcentral.com)

AVM induced the release of cleaved PARP and cleaved caspase‑3 caused apoptosis and inhibited the viability of these cells. (spandidos-publications.com)

Natural killer (NK) cells are innate lymphocytes that play a pivotal role in the immune surveillance and elimination of transformed or virally infected cells. (frontiersin.org)

This enhances the direct killing of tumor cells by drug molecules while minimizing the potential toxicity to normal cells. (biomed.news)

Results Natural killer cells from healthy donors and myeloma patients expanded a median of 804- and 351-fold, respectively, without significant T-cell expansion. (haematologica.org)
Our results demonstrated high potential of IL-15 for NK cell expansion, while IL-21 triggered NK cell maturation and functionality. (frontiersin.org)
Here, we describe the synthesis of chloroquine analogs and show the results of mitochondrial ATP inhibition testing. (biomed.news)

Serine-threonine kinases are involved in cell signaling and help regulate developmental processes linked to multicellularity in the Myxococcota. (sciencegate.app)

Then, the MVB's can either fuse with lysosomes resulting in degradation of intra-luminal contents or they can secrete their content as exosomes outside the donor cells. (bbrc.in)

Laser scanning microscopy showed a high capacity to absorb NSC631570 in malignant cells while absorption in normal cells was considerable lower under the same experimental conditions (36). (ukrin.com)

Moreover, NK cells are poised to release cytokines such as IFN- γ , TNF-α and growth factors that can initiate inflammatory responses mediated by both the innate and the adaptive arm of the immune system. (frontiersin.org)

The amount of melanin synthesis by MeOH and CH 2 Cl 2 fractions and mushroom tyrosinase activity by the MeOH fraction declined in B16F10 cells. (ac.ir)
Implications of Heparanase on Heparin Synthesis and Metabolism in Mast Cells. (uu.se)

Anatomy17

Organisms10

Diseases20

Chemicals and Drugs120

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes60

Disciplines and Occupations1

Information Science3

Proliferation23

Apoptosis in human1

Differentiation17

Inhibit9

Vitro9

Cytotoxic3

IC503

Apoptotic6

Cytotoxicity3

Myeloid10

Morphology2

Tumor cell3

Pathway5

Potent2

Life Sciences1

Assay5

Mechanism5

Inhibitors2

U9374

Induce1

Autophagy2

Line17

Human16

Survival6

Pathways4

Pharmacological1

Flow2

Accumulation1

Inhibits1

Arrest2

Dose-dependent manner1

Proteins3

Topoisomerase1

Concentrations6

Angiogenesis1

Lines4

Phenotype2

Significantly3

Resistant3

Mitochondrial membrane1

Induces1

Gene6

Metabolic1

Therapeutic3

Expression7

Leukemic cells1

Cancer8

Viability1

Virally infected1

Enhances1

Results3

Kinases1

Degradation1

Malignant cells1

Initiate1

Synthesis2