MolecularAntibodyCopiesMonoclonalTumor supprAlloreactive T cellsMammalian cellsClonalProteinAntigenGeneticVectorsBiologyBacteriaTissueRecombinant2023TissuesTherapeuticPositionalMutationsCDNAReproductiveEmbryonicGenomeCRISPRSequenceSomaticTransfectionPhenotypesTumorsPropagateDollyFragmentsIsolationLeukemic cellsSignaling pathwayDilutionIsolateImmuneCellularDiseasesAlterationsSpindleProteinsMiceCharacteristicsVectorGenomicTherapiesDendriticRenal cell carc2022ProcessesReceptorHeterogeneityStem CellsProgenitor cellsLund Stem Cell CenterSpeciesColonyGenetically identicalNaturallyEfficiencies
Molecular9
- Amy Radunz, a nutritionist from the department of animal sciences at the University of Wisconsin in River Falls, has partnered with molecular biologist Hasan Khatib, an associate professor at University of Wisconsin-Madison, to ask how differences in maternal diet during pregnancy induce gene expression and DNA methylation changes in fetal tissues in sheep. (genengnews.com)
- So that it features which are useful for molecular cloning experiments. (biologyexams4u.com)
- Despite improvements in the CRISPR molecular toolbox, identifying and purifying properly edited clones remains slow, laborious, and low-yield. (nature.com)
- His research centers on a molecular and cellular understanding of CD4 T cell responsiveness in mice and man. (wikipedia.org)
- Generation and characterization of infectious molecular clones of transmitted/founder HIV-1 subtype C viruses. (harvard.edu)
- The use of molecular profiling to predict survival after chemotherapy for diffuse large B cell lymphoma. (nature.com)
- Cloning in biotechnology refers to processes used to create copies of DNA fragments ( molecular cloning ), cells (cell cloning), or organisms . (wikiquote.org)
- This is achieved through mechanistic studies of functionally important epigenetic "driver" genes and molecular pathways altered by specific cancer risk agents and by the application of cutting-edge epigenomics in conjunction with unique biospecimens from population-based cohorts (Figure 1). (who.int)
- The molecular interactions of genes and gene products underlie fundamental questions of biology. (lu.se)
Antibody14
- T-cells play a crucial role in the immune response to pathogens by mediating antibody formation and clearance of infected cells, and by defining an overall response strategy. (elifesciences.org)
- When genome editing induces a phenotypic alteration that is detectable by fluorescence (i.e., cell-surface expression of a target that can be non-lethally assessed with fluorescently labeled antibody), FACS provides a method of enriching edited cells 9 , narrowing the number of clones to propagate and analyze. (nature.com)
- This approach will permit analysis of B cell clonal ontogeny, antibody diversity and lymphoma cell progression and heterogeneity. (lu.se)
- In this approach, researchers first collect antibody-producing cells from mice exposed to an antigen of interest and fuse them to myeloma cells, forming so-called hybridomas that can survive for a long time in culture. (the-scientist.com)
- By screening single hybrid cells for those that produce the antibody of interest, researchers can develop a cell line generating virtually unlimited amounts of highly specific antibodies. (the-scientist.com)
- For each isolated cell, they separately clone the two genes encoding the heavy and light chains that compose an antibody, and that vary in sequence from cell to cell. (the-scientist.com)
- Once researchers have cloned the pairs of heavy- and light-chain genes naturally found in the cells, they can produce recombinant versions of each antibody in the lab. (the-scientist.com)
- This technique, commonly referred to as expression cloning, "is powerful because you're not limited-any B cell that you can isolate, in any context, you can make an antibody from," Wilson says. (the-scientist.com)
- The issue with it is that there are a lot of steps for each cell to clone those antibody genes. (the-scientist.com)
- It would be extremely laborious to use this strategy to try to identify an antibody against an antigen of interest using the blood of a person without an active immune response, he says, because millions of memory B cells are present in the blood, each of which is uniquely specific for a different antigen. (the-scientist.com)
- CHARTING THE COURSE: B cells grow in culture in individual wells over a layer of feeder cells, and culture media from cells is screened for antibody-binding specificity or neutralizing activity. (the-scientist.com)
- The immortalization of antibody-producing cells from patients infected with HIV can be inefficient, and even when cells are successfully immortalized, some clones tend to be unstable. (the-scientist.com)
- But rather than cloning the antibody heavy- and light-chain genes from each cell, as in a typical expression cloning set-up, Connors's group distributed the isolated cells in 384-well plates, where they cultured them in the presence of feeder cells and signaling molecules that help to keep the B cells alive and also stimulate them to secrete antibodies. (the-scientist.com)
- we identified a novel gene encoding a 24 amino acid peptide named Humanin (HN) that suppressed neuronal death induced by any type of FAD genes thus far examined, toxic Aβ peptides, and anti-APP antibody (Hashimoto et al. (jneurosci.org)
Copies5
- Cloning vectors are used for obtaining millions of copies of the cloned DNA fragments. (biologyexams4u.com)
- gene cloning ( DNA cloning ) The production of exact copies ( clones ) of a particular gene or DNA sequence using genetic engineering techniques. (encyclopedia.com)
- Engineers take the gene from the bacteria (Bt) and make millions of copies. (responsibletechnology.org)
- They literally put millions of copies of the gene into a gun that coats tungsten or gold particles. (responsibletechnology.org)
- Levels of gene expression are measured using a preparation of fluorescently labeled tissue RNA (copies of DNA), together with reference RNA labeled with a different fluorochrome, hybridized onto the slides. (lu.se)
Monoclonal4
- Monoclonal Anti-MUC1 (mouse IgG1 isotype) is derived from the hybridoma 1D1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with semi-purified MUC1 from pleural effusion of a breast cancer patient. (sigmaaldrich.com)
- 1996) Monoclonal T cells identified in early NOD islet infiltrates. (wikipedia.org)
- The approach was applied to five different hybridomas producing human monoclonal antibodies and variable regions for both bold gamma and mu heavy chain and kappa and lambda light chain genes were successfully cloned. (lu.se)
- However, spontaneous antitumor CD8+ T cell immunity in peripheral blood and tumors was restricted to a few clonal neoantigens featuring an oligo-/monoclonal T cell-receptor (TCR) repertoire. (nih.gov)
Tumor suppr1
- Using a lentiviral vector containing either H-Ras oncogene and a hairpin targeting p53 or both tumor suppressor genes, NF-1 and p53, Dr. Friedmann-Morvinski can induce the formation of a glioma tumor in mice injected in the brain. (genengnews.com)
Alloreactive T cells1
- Clonal dynamics of alloreactive T cells in kidney allograft rejection after anti-PD-1 therapy. (harvard.edu)
Mammalian cells1
- SCNT is a method of cloning mammalian cells that can be used to create personalized embryonic stem cells from an adult animal or human. (news-medical.net)
Clonal6
- Here, we establish a method to enable clonal isolation, selection, and expansion of properly edited cells, using OptoElectroPositioning technology for single-cell manipulation on a nanofluidic device. (nature.com)
- Each colony is split and sequentially exported for on-target sequencing and further off-chip clonal expansion of the validated clones. (nature.com)
- A mixed-effects stochastic model reveals clonal dominance in gene therapy safety studies. (harvard.edu)
- Gene transfer of TCRαβ heterodimers specific for clonal neoantigens confirmed correct TCR clonotype assignments based on high-throughput TCRBV CDR3 sequencing. (nih.gov)
- Our findings implicate immunological ignorance of clonal neoantigens as the basis for ineffective T cell immunity to melanoma and support the concept that therapeutic vaccination, as an adjunct to checkpoint inhibitor treatment, is required to increase the breadth and diversity of neoantigen-specific CD8+ T cells. (nih.gov)
- Proof of antiviral clonal T-cells in leukemia patients during TKI therapy. (helsinki.fi)
Protein6
- Expression vectors are designed for expressing the protein encoded by the inserted gene. (biologyexams4u.com)
- Cosmid can be defined as the hybrid vectors derived from plasmids that contain cos site of phage which is essential for packaging of nucleic acid into protein coat plus essential features of plasmids and several unique restriction sites for the insertion of DNA to be cloned. (biologyexams4u.com)
- The specificity of T-cells is determined by the T-cell receptor (TCR), a heterodimer of alpha and beta protein chains. (elifesciences.org)
- Topics covered will include cell membrane structure and function, metabolism, cell motility and division, genome structure and replication, the regulation of gene expression and protein production, genotype to phenotype relationship, and basic principles of inheritance. (middlebury.edu)
- the gene produced 'GNA lectin,' a protein that kills insects. (bibliotecapleyades.net)
- Identified gene candidates will be studied further in the context of determining sensitivity of protein degrader drugs which are currently emerging as alternative therapies for leukemia. (lu.se)
Antigen6
- 1981) Antigen-reactive T cell clones. (wikipedia.org)
- I. Transcomplementing hybrid I-A-region gene products function effectively in antigen presentation. (wikipedia.org)
- Genotypic pattern observed in small plaque parapsoriasis is similar to that observed in chronic dermatitis, and the pattern of clonality of T cells is consistent with the response of a specific subset of T cells that have been stimulated by an antigen. (medscape.com)
- More recently, Wilson and others have devised ways to isolate single, antigen-specific B cells from the blood of human donors with active immune responses against a particular pathogen or vaccine. (the-scientist.com)
- This technique is best for "when you have an enriched population of antigen-specific cells-they're not there, except for a short time after vaccination or infection," says Wilson. (the-scientist.com)
- While previous researchers have obtained HIV-neutralizing antibodies using fluorescent probes to pull out antigen-specific memory B cells from a sample of blood, Connors and his team wanted to discover new HIV-neutralizing antibodies, without being limited only to those that are specific for known antigens. (the-scientist.com)
Genetic10
- Cloning vectors (Cloning vehicles) are used for creating genomic libraries, preparing probes, genetic engineering, or other basic studies. (biologyexams4u.com)
- Globally, there are several cloning programmes that aim to increase the size of rapidly-dwindling populations of endangered species such as African wildcats, and maintain genetic diversity through one-off clonings of individuals that haven't bred . (newscientist.com)
- There are widespread efforts to use this approach in clinically relevant systems to model genetic disorders 4 and for gene therapy to correct disease-driving mutations 5 . (nature.com)
- If this gene is defective, the person carrying the genetic defect will develop Alzheimer's. (medicalxpress.com)
- The result is a reconstructed embryo, i.e. a cloned egg, which develops into a new individual with the same genetic characteristics as the gene-edited skin cell. (medicalxpress.com)
- Cloning describes the processes used to create an exact genetic replica of another cell, tissue or organism. (medlineplus.gov)
- The copied material, which has the same genetic makeup as the original, is referred to as a clone. (medlineplus.gov)
- At a time when the idea that cancer had a genetic basis was widely disbelieved, Nowell's results provided the first clear evidence that a particular genetic defect in a single chromosome can lead to a population or clone of identical cells that accumulate in numbers to form a deadly malignancy. (laskerfoundation.org)
- Genetic pollution persists because it corrupts the gene pool and we have no technology to clean it up. (responsibletechnology.org)
- Through the cloning of Dolly, we learned that the cell nucleus contains all the genetic information needed for the cell to develop into any type of cell. (lu.se)
Vectors7
- Any DNA molecule that has the ability to replicate inside the host to which the desired gene is integrated for cloning- include plasmids, bacteriophages, cosmids, BAC, yeast vectors, shuttle vectors etc. (biologyexams4u.com)
- The cloned genes in these vectors are not expected to express themselves at the transcription or translation level. (biologyexams4u.com)
- The most important requirement for recombinant DNA technology is the cloning vector and expression vectors. (biologyexams4u.com)
- Cosmids are the novel cloning vectors which possess properties of both plasmid and phage. (biologyexams4u.com)
- These fragments are then inserted into cloning vectors , such as bacterial plasmids or bacteriophages, which transfer the recombinant DNA to suitable host cells, such as the bacterium E. coli. (encyclopedia.com)
- Using PCR, researchers then amplify the heavy and light chain genes from antibodies of interest, clone them into expression vectors, and sequence them. (the-scientist.com)
- The expression vectors are transfected into cells, and the resulting antibodies are purified and tested for activity. (the-scientist.com)
Biology2
- In biology , cloning is the process of producing similar populations of genetically identical individuals that occurs in nature when organisms such as bacteria , insects or plants reproduce asexually . (wikiquote.org)
- As the sequencing and gene annotation projects of entire genomes of many species are headed towards completion (see, e.g. [13] ), massive mapping efforts in biology are now focused on how the genes interact. (lu.se)
Bacteria4
- Should have one or more marker genes for identification ad isolation of subpopulation of bacteria containing vector. (biologyexams4u.com)
- human insulin, for example, is now produced by bacteria containing the cloned insulin gene. (encyclopedia.com)
- Typically, scientists transfer genes from one species (like bacteria or viruses) and force them into the DNA of other species (like soybeans or corn. (responsibletechnology.org)
- They use bacteria to infect the cell, carrying the gene with it. (responsibletechnology.org)
Tissue11
- The Wakayama team used a modified version of a cloning technique in which the nucleus of a mouse cell - in this case a cell from dead tissue that has been frozen and then thawed - is injected into a mouse egg that has had its nucleus removed. (newscientist.com)
- In a surprise finding, the Wakayama team discovered that it was easiest to create clones from brain tissue, even though clones have never been created from living brain cells. (newscientist.com)
- Wakayama speculates that freezing and thawing the tissue somehow makes it easier to "reprogram" the brain cell nucleus. (newscientist.com)
- Brain tissue is also high in sugars, which can protect cells when they freeze. (newscientist.com)
- But this is the first time animals have been cloned from lumps of tissue frozen without the use of chemicals that might protect the cells from damage. (newscientist.com)
- Researchers hope to use these cells to grow healthy tissue to replace injured or diseased tissues in the human body. (medlineplus.gov)
- If there are intact cells in this tissue they have been 'stored' frozen. (wikiquote.org)
- It is also our view that there are no sound reasons for treating the early-stage human embryo or cloned human embryo as anything special, or as having moral status greater than human somatic cells in tissue culture. (wikiquote.org)
- A blastocyst (cloned or not), because it lacks any trace of a nervous system, has no capacity for suffering or conscious experience in any form - the special properties that, in our view, spell the difference between biological tissue and a human life worthy of respect and rights. (wikiquote.org)
- I am currently conducting surgeries to gain insight into chronic rejection after liver transplants in rat liver transplant models, as well as high-dimensional mass cytometry (CyTOF) analysis using the samples of vascularized composite tissue transplant to discern cell populations and subsets involved in the rejection. (stanford.edu)
- Typically when data are presented, the fluor- escent intensity from the tissue is pseudo-colored red and the intensity from the reference green, and the logarithmic ratio of background corrected red and green intensities for each gene (spot) is subject to analysis (see Fig. 1 ). (lu.se)
Recombinant1
- Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (harvard.edu)
20231
- Retrieved on December 04, 2023 from https://www.news-medical.net/life-sciences/Cloning-Human-Cells.aspx. (news-medical.net)
Tissues5
- The study also looked at the impact of paternally expressed genes: IGF2, PEG1, PEG3, DLK1, and DIO3 for transcriptomic and epigenomic alterations of the imprinted genes in fetal tissues. (genengnews.com)
- Cloning of human cells is a technology that holds the potential to cure many diseases and provide a source of exactly matched transplant tissues and organs. (news-medical.net)
- Inspired from developmental processes, human mesenchymal cell lines can be programmed to form cartilage, bone and bone marrow tissues in vitro and in vivo. (lu.se)
- C. Peterson, M. Ringne´r / Artificial Intelligence in Medicine 28 (2003) 59-74 structure and function, cellular metabolism, development of cells and tissues, and response of organisms to their environments. (lu.se)
- Since genes are composed of a given alphabet (A, T, C, G) with fixed pairing properties A-T and C-G, probes can be constructed that attract gene transcripts extracted from cell tissues and cultures. (lu.se)
Therapeutic4
- The therapeutic potential of cloned human cells has been demonstrated by another study using human oocytes to reprogram adult cells of a type 1 diabetic. (news-medical.net)
- Although attempts have not yet been made to create a therapeutic transplant from embryonic stem cells, the methods have been developed to allow the creation of functional, mature cells using human cell cloning technology. (news-medical.net)
- Therapeutic cloning, which creates embryonic stem cells . (medlineplus.gov)
- Can these cells be used as a therapeutic for EBV+ lymphomas? (stanford.edu)
Positional1
- See also positional cloning . (encyclopedia.com)
Mutations6
- The cloned pigs were born with a mutation in the gene SORL1, which is interesting because the mutations are found in up to 2-3% of all early onset Alzheimer's cases in human beings. (medicalxpress.com)
- The group of patients who carry mutations of SORL1 is far larger than the group of patients who have errors in the three other known genes," says Olav Michael Andersen. (medicalxpress.com)
- Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib. (nature.com)
- Somatic mutations in in expanded T cell clones were discovered in patients with immune-mediated aplastic anemia. (helsinki.fi)
- T cells with somatic mutations on JAK-STAT and MAPK pathway genes were characterized by single-cell transcriptomics. (helsinki.fi)
- Next, we discuss mutations of genes related to autophagy and the UPR in CRC. (bvsalud.org)
CDNA1
- cDNA encoding variable regions could be amplified from single hybridoma cells isolated by micromanipulation. (lu.se)
Reproductive1
- Reproductive cloning is expensive and highly inefficient. (wikiquote.org)
Embryonic10
- The resulting embryo was then used to create embryonic stem cells, capable of generating every cell type in the body, and the nuclei of these cells were injected into other eggs to produce clones. (newscientist.com)
- Embryonic stem cells are immortal, and have the potential to develop into any type of adult cell, even after months growing in culture dishes. (news-medical.net)
- By transferring adult cell DNA into an embryonic stem cell, it is possible to create a line of immortal embryonic cells that are able to develop into any type of adult cell, genetically identical to the donor. (news-medical.net)
- The cloning method is based on the fact that cytoplasmic factors in mature, metaphase II oocytes are able to reset the identity of a transplanted adult cell nucleus to an embryonic state. (news-medical.net)
- In humans, a major roadblock in achieving successful SCNT leading to embryonic stem cells has been the fact that human SCNT embryos fail to progress beyond the eight-cell stage. (news-medical.net)
- This was believed to be due to an inability to activate certain embryonic genes. (news-medical.net)
- They derived several human embryonic stem cell lines from these cloned embryos whose DNA was an exact match to the adult cell that donated the DNA. (news-medical.net)
- This was the first successful reprogramming of human somatic cells into embryonic stem cells using a cloning technique, SCNT. (news-medical.net)
- Dermal fibroblasts were taken from a 35-year old male and a 75-year-old male and used to create embryonic stem cells. (news-medical.net)
- The adult cell nuclei were transferred into metaphase-II stage human oocytes, producing a karyotypically normal diploid embryonic stem cell line from each of the adult male donor cells. (news-medical.net)
Genome3
- Scientists armed with whole genome sequence data can now ask questions about genome structure and organization that impact gene expression. (genengnews.com)
- In the upstream step, delivery of genome editing machinery to the cell type of interest generates efficient and specific edits. (nature.com)
- Therefore, the development of a method that allows screening of edited cells and minimizes cell manipulation and hands-on culturing would constitute a significant addition to the current genome engineering toolbox. (nature.com)
CRISPR2
- Progress has been made to enhance the efficiency of HDR-based editing 8 , however, a technology to identify cells with desired mono- or bi-allelic edits is urgently needed to realize the full potential of CRISPR. (nature.com)
- In this study, the researchers had previously used CRISPR-Cas9-based gene editing to destroy the SORL1 gene in a skin cell taken from a minipig of the Göttingen breed. (medicalxpress.com)
Sequence2
- Improved access to bioinformatics tools enables scientists to process, manage, and store raw data, annotate sequences to define genes, and quantitate RNA sequence data to get insight into gene expression patterns. (genengnews.com)
- Estimating the fraction of the T-cell response directed against the single immunodominant epitope, we identify the sequence features of TCRs that define the high precursor frequency of the two major TCR motifs specific for this particular epitope. (elifesciences.org)
Somatic1
- One cloning technology that has been developed for mammalian and human cells is somatic cell nuclear transfer (SCNT). (news-medical.net)
Transfection1
- The neoplastic potential of transformed cells was determined via soft agar cloning and gene transfection analysis. (cdc.gov)
Phenotypes1
- Using TCR alpha and beta repertoire sequencing for T-cell subsets, as well as single-cell RNAseq and TCRseq, we track the concentrations and phenotypes of individual T-cell clones in response to primary and secondary yellow fever immunization - the model for acute infection in humans - showing their large diversity. (elifesciences.org)
Tumors5
- Dinorah Friedmann-Morvinski, a post-doctoral fellow in Inder Verma's lab at the Salk Institute, has shown how tumors originated from mature neurons dedifferentiate into glioma stem-like progenitor cells. (genengnews.com)
- In cancer patients dendritic cells may become dysfunctional or excluded from tumors. (lu.se)
- The reprogrammed cancer cells thereby lose their ability to become tumors, and instead activate the immune system to respond to mutated proteins in the tumor. (lu.se)
- Our findings are paving the way for the development of a gene therapy for cancer where the three molecules are delivered directly into tumors in patients eliciting a response independently of the tumor type. (lu.se)
- diagnostic prediction of small round blue cell tumors (SRBCT) of childhood and determining the estrogen receptor (ER) status of sporadic breast cancer. (lu.se)
Propagate1
- Developments in biotechnology have raised new concerns about animal welfare, as farm animals now have their genomes modified (genetically engineered) or copied (cloned) to propagate certain traits useful to agribusiness, such as meat yield or feed conversion. (wikiquote.org)
Dolly3
- This has led to a lot of interest in SCNT, which is best known as the method used to pioneer whole animal cloning technology, such as Dolly the sheep. (news-medical.net)
- The most famous clone was a Scottish sheep named Dolly. (medlineplus.gov)
- He was in high school when he heard about Dolly, one of the world's most extreme examples of cell reprogramming. (lu.se)
Fragments2
- A vector used to clone DNA fragments of 100-300 kb insert size in E.coli cells. (biologyexams4u.com)
- The DNA containing the target gene(s) is split into fragments using restriction enzymes . (encyclopedia.com)
Isolation1
- Isolation of T cell clones by limiting dilution from tumor-infiltrating lymphocytes (TILs) permitted functional validation regarding neoantigen specificity. (nih.gov)
Leukemic cells3
- One day while "diddling around with leukemic cells in culture," and rinsing them with tap water, Nowell noted that cells were dividing. (laskerfoundation.org)
- I came to realize that there were many questions about chromosome changes in patients that would be rewarding to study," noted Rowley, and for the next decade she labored over the microscope looking at chromosomes in leukemic cells. (laskerfoundation.org)
- The thesis work would focus on identification of genes that are essential for the optimal functioning of the ubiquitin-proteasomal system in leukemic cells. (lu.se)
Signaling pathway1
- Silencing oncogene cell division cycle associated 5 induces apoptosis and G1 phase arrest of non-small cell lung cancer cells via p53-p21 signaling pathway. (nih.gov)
Dilution1
- Selection of edited cell clones currently relies on limiting dilution or fluorescence-activated cell sorting (FACS)-based single-cell sorting to isolate single cells. (nature.com)
Isolate3
- As a consequence, investigators often need to isolate a large number of clones and then proceed with tedious and time-consuming efforts to expand all of them individually. (nature.com)
- Colorimetric Barcoding to Track, Isolate, and Analyze Hematopoietic Stem Cell Clones. (harvard.edu)
- Connors and his team first used flow cytometry to isolate B cells from an HIV-infected donor. (the-scientist.com)
Immune12
- The diverse repertoire of T-cell receptors (TCR) plays a key role in the adaptive immune response to infections. (elifesciences.org)
- The resulting diverse naïve repertoire contains T-cell clones that recognize epitopes of yet unseen pathogens, and can participate in the immune response to infection or vaccination. (elifesciences.org)
- Characterization of immune cell contexture in adult ALL bone marrow microenvironment. (helsinki.fi)
- In addition to low success rates, cloned animals tend to have more compromised immune function and higher rates of infection, tumor growth, and other disorders. (wikiquote.org)
- Their white blood cells responded to an immune challenge more slowly, indicating immune system damage. (bibliotecapleyades.net)
- Pereira's research also involves reprogramming skin cells into dendritic cells, which are the sentinels of the immune system. (lu.se)
- They play a key role in orchestrating the immune response by "informing" other immune cells about the threat from foreign substances in the body. (lu.se)
- Therefore, new ways are needed to reprogram other cells into immune cells with the same capacity as the "natural" dendritic cells. (lu.se)
- In other words, using the molecules as a trojan horse to "force" cancer cells to develop into immune cells. (lu.se)
- We know that innate immune cells are required for blastema progenitor cell formation, but are lacking an understanding of the immune cell processes and functions that are required for inducing and maintaining these progenitors cells. (lu.se)
- This project will delve into innate immune cell function, establishing in vitro and in vivo systems test innate immune cell function in regeneration. (lu.se)
- This will be coupled with next generation sequencing based approaches to understanding the consequences of perturbing immune cell function. (lu.se)
Cellular3
- [ 18 ] The strategy of triggering cells to enter into persister cell fate is to overproduce the toxins that inhibit cellular processes and growth, which is mediated by toxin-antitoxin modules. (medscape.com)
- These molecules control several aspects of neural cell life including axonal growth, cellular migration, and cell death, and therefore, are of increasing interest in the field of spinal cord injury (SCI). (bvsalud.org)
- Filipe Pereira has found his niche at the interface of cellular reprogramming and blood cell identity. (lu.se)
Diseases4
- Researchers have also previously developed pig models for Alzheimer's and other diseases by means of cloning. (medicalxpress.com)
- These cells have been sought after as potential therapies for diseases ranging from heart disease to Parkinson's to cancer. (news-medical.net)
- The initiating cause of parapsoriasis is unknown, but the diseases likely represent different stages in a continuum of lymphoproliferative disorders from chronic dermatitis stimulated by activated T cells to frank malignancy of cutaneous T-cell lymphoma (CTCL). (medscape.com)
- Claims that you could clone individual treatments of human beings to treat common diseases like diabetes, suggests you need a huge supply of human eggs. (wikiquote.org)
Alterations1
- Nowell, a tumor biologist in the pathology department at the University of Pennsylvania School of Medicine, was interested in the relationship between cancer and alterations in genes (although he had no proof there was one). (laskerfoundation.org)
Spindle1
- Those were spindle removal, donor cell fusion, and cytoplast activation. (news-medical.net)
Proteins1
- The Mucin 1 (MUC1) gene encodes a membrane bound glycosylated phosphoprotein, belonging to the family of mucins which are heavily glycosylated proteins. (sigmaaldrich.com)
Mice2
- Healthy mice have been cloned from cells from dead mice that had been frozen for 16 years, raising the possibility that endangered species could be cloned from old carcasses that have been tossed in freezers, rather than from living cells frozen using elaborate techniques. (newscientist.com)
- Other teams have already cloned mice from previously frozen dead cells . (newscientist.com)
Characteristics2
- From this study the authors conclude that glass fibers are capable of transforming BALB/c-3T3 cells, and that such transformed cells exhibit characteristics suggestive of neoplastic potential. (cdc.gov)
- Blood stem cells lose important characteristics when they are cultured, while skin cells are both easily accessible and can be easily expanded in test tubes. (lu.se)
Vector5
- What is a cloning vector? (biologyexams4u.com)
- The vector must be able to replicate in host cells. (biologyexams4u.com)
- YAC (Yeast Artificial Chromosome): A vector of hundreds to kilobases long used for cloning of DNA fragment. (biologyexams4u.com)
- First artificial cloning vector (1977) constructed by Boliver & Rodriguez from E .coli plasmid. (biologyexams4u.com)
- It is a 4362 kb long widely used cloning vector. (biologyexams4u.com)
Genomic1
- A genomic strategy to refine prognosis in early stage non small cell lung cancer. (nature.com)
Therapies3
- But SCNT can also be used to clone human cells for transplant or other therapies. (news-medical.net)
- [ 1 ] With the range of histology detected, treatments are variable but therapies that affect T cells can be effective. (medscape.com)
- Inhibiting these molecules has been shown to increase neuroprotection, enhance axonal regeneration, and facilitate the implementation of cell replacement therapies. (bvsalud.org)
Dendritic4
- Ultraviolet radiation shapes dendritic cell leukaemia transformation in the skin. (harvard.edu)
- Mature dendritic cell (mDC) vaccination with tumor-encoded amino acid-substituted (AAS) peptides revealed diverse neoantigen-specific CD8+ T responses, each composed of multiple TCR clonotypes. (nih.gov)
- By using skin cells as the starting cell-type, he managed to identify the three molecules needed to "program" dendritic cells in just nine days. (lu.se)
- In the next step, he used the same molecules to reprogram cancer cells into dendritic cells. (lu.se)
Renal cell carc1
- Loss of cell division cycle‑associated 5 promotes cell apoptosis by activating DNA damage response in clear cell renal cell carcinoma. (nih.gov)
20221
- Cell Reports Medicine (2022). (medicalxpress.com)
Processes1
- Current terminology of parapsoriasis refers to 2 disease processes that are caused by T-cell-predominant infiltrates in the skin. (medscape.com)
Receptor2
- Multiple dominant clones can be detected by polymerase chain reaction (PCR) of T-cell receptor gene usage, which supports a reactive process. (medscape.com)
- Southern blot analysis of T-cell receptor genes from parapsoriasis does not identify a dominant clone of T cells. (medscape.com)
Heterogeneity1
- The impact of intratumoral heterogeneity (ITH) and the resultant neoantigen landscape on T cell immunity are poorly understood. (nih.gov)
Stem Cells4
- The mitochondrial DNA of the stem cells, however, matched the donor egg's mitochondrial DNA. (news-medical.net)
- Possibility to work with primary human hematopoietic stem cells. (lu.se)
- He has succeeded in converting human skin cells into blood stem cells, which is a first step towards creating fully functional blood stem cells in test tubes. (lu.se)
- Reprogramming skin cells to blood stem cells could potentially provide an unlimited source of cells for transplantation to patients with blood disorders. (lu.se)
Progenitor cells1
- This process relies on the formation of a blastema, a pool of progenitor cells that is formed in response to amputation. (lu.se)
Lund Stem Cell Center1
- This is a tool to help students find the right project for their Bachelor's and Master's thesis at Lund Stem Cell Center. (lu.se)
Species2
- What's more, most conservationists agree that cloning should be considered only as a last resort for species such as the northern white rhino, where all other attempts at conservation have failed, says Paul Bartels, manager of BioBankSA at the National Zoological Gardens of South Africa in Pretoria. (newscientist.com)
- Given that we have an efficiency of 1% cloning for livestock species and if only one in a thousand cells are viable then around 100,000 cells would need to be transferred. (wikiquote.org)
Colony2
- thus, a bacterial host will give rise to a colony of cells containing the cloned target gene. (encyclopedia.com)
- Biofilms contain a small reversible subpopulation of so-called persister cells that adopt a slow- or nongrowing lifestyle through the emergence of small colony variants and are highly tolerant to extracellular stresses, such as antibiotic treatment. (medscape.com)
Genetically identical1
- A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. (harvard.edu)
Naturally1
- Bt Crops- Engineers insert a naturally occurring toxin (Bacillus thuringiensis) into plant cells. (responsibletechnology.org)
Efficiencies1
- Using this method, single-clone editing efficiencies, including the rate of mono- and bi-allelic indels or precise nucleotide replacements, can be assessed within 10 days from Cas9 ribonucleoprotein introduction in cells. (nature.com)