Cell gene expression regulation. Medical search. Frequent questions

These findings suggest that, in C6 glioma cells, c-fos mRNA induction induced by EAAs is not mediated by phosphorylation of ERK and CREB proteins. (kyobobook.co.kr)
Gene expression is regulated in part by the interactions of genomic DNA with the packaging histone proteins. (wayne.edu)
Tumor suppressor genes encode proteins that normally provide negative control of cell proliferation. (medscape.com)
Translation of these hexanucleotide repeat sequences gives rise to dipeptide-repeat proteins, which form intracellular protein aggregates in neuronal and non-neuronal cells of affected patients. (europa.eu)
This paper reports that certain of these dipeptide repeat proteins interfere with protein synthesis in affected cells, and this molecular derailment may contribute to motor neuron degenetion in C9orf72-ALS patients. (europa.eu)
This balance is maintained by the combinatorial activity of transcription factors and chromatin proteins that dictate the transcription of coding gene networks instructing fate choice decisions. (upenn.edu)
Membrane proteins implicated in long-chain fatty acid uptake by mammalian cells: CD36, FATP and FABPm. (springer.com)
New non-coding elements are being discovered which, rather than encoding for proteins, typically have regulatory roles in gene expression. (otago.ac.nz)
The MBD5 gene provides instructions for a protein that likely regulates the activity (expression) of genes, controlling the production of proteins that are involved in neurological functions such as learning, memory, and behavior. (medlineplus.gov)
It is likely that any changes in MBD5 protein levels impair its regulation of gene expression, leading to the uncontrolled production of certain proteins. (medlineplus.gov)
This process is mediated by a complex molecular machinery known as the spliceosome, which enables the production of multiple and functionally distinct proteins from single genes. (lu.se)
Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)
Our project offers a unique opportunity for Master's student to gain hands-on experience focused on transcriptional regulators with importance in expression of genes of outer membrane proteins that are essential in host-pathogen interaction. (lu.se)

To study the cellular mechanisms for this post-transcriptional control and to determine the contribution of mRNA stability to GAP-43 gene expression, we examined the effects of several agents that affect PC12 cell differentiation on the level of induction and rate of degradation of the GAP-43 mRNA. (rupress.org)
The phorbol ester-induced stabilization of GAP-43 mRNA was blocked by the protein kinase inhibitor polymyxin B and was partially inhibited by dexamethasone, an agent that blocks GAP-43 expression and neuronal differentiation in PC12 cells. (rupress.org)
The tumor-associated stromal cells were found to have decreased expression of genes involved in smooth muscle differentiation, and those detected in prostate but not bladder. (nih.gov)
In multicellular organisms, gene regulation drives cellular differentiation and morphogenesis in the embryo, leading to the creation of different cell types that possess different gene expression profiles from the same genome sequence. (wikipedia.org)
Single-Cell Transcriptomics Reveals that Differentiation and Spatial Signatures Shape Epidermal and Hair Follicle Heterogeneity. (crossref.org)
We have investigated in detail how the remote regulatory elements of the alpha-globin cluster become activated as hematopoietic stem cells (HSCs) undergo commitment, lineage specification, and differentiation to form red blood cells. (ox.ac.uk)
The authors sought to identify mechanisms driving the differentiation of mucus cells during the bronchiolisation process. (bmj.com)
Methods Pathways governing airway mucus cell differentiation include SRY (sex determining region Y)-box 2 (SOX2), Notch, forkhead box A3(FOXA3)/SAM pointed domain containing ETS transcription factor (SPDEF), epidermal growth factor (EGF) and the EGF-related neuregulins NRG1α and NRG1β. (bmj.com)
In this work, pathways governing mucus cell differentiation were investigated in lung tissues from patients with IPF and other chronic pulmonary disorders. (bmj.com)
Neuregulin1α, which drives mucus cell differentiation in vitro, was expressed in normal airway submucosal glands and in lungs from patients with IPF. (bmj.com)
Activation of abnormal respiratory epithelial differentiation programs may contribute to the expression of MUC5B and bronchiolisation of the distal lung, a salient feature of IPF. (bmj.com)
C: SVZ neural stem cell (NSC) cultures in self-renewal (neurosphere formation) and differentiation. (wjgnet.com)
TET1 plays a critical role in intestinal epithelial differentiation and development, and this is also correlated with increased hydroxymethylation in terminally differentiated epithelial cells. (intechopen.com)
Recent advances in live imaging and genetics of mammalian division, movement and cell differentiation leading to development which integrate observations of biochemical tissue formation [14 ]. (lu.se)
Atherosclerosis starts with dysfunctional changes in the endothelium induced by disturbed shear stress which can lead to endothelial and platelet activation, adhesion of monocytes on the activated endothelium, and differentiation into proinflammatory macrophages, which increase the uptake of oxidized LDL (oxLDL) and turn into foam cells, exacerbating the inflammatory signalling. (hindawi.com)
The MBD5 protein also seems to play a role in the growth and division (proliferation) and maturation (differentiation) of various types of cells. (medlineplus.gov)
The 2024 edition of the Stem Cell Stars seminar series will explore diverse topics from the dynamic processes of cell differentiation to the latest advances in stem cell engineering. (lu.se)

Often, DNA methylation and histone deacetylation work together in gene silencing. (wikipedia.org)
Histone variant H2A.Z marks the 5' ends of both active and inactive genes in euchromatin. (broadinstitute.org)
Active genes are tri-methylated at K4 of histone H3. (broadinstitute.org)
Although the transcriptional activity of the histone genes (except H5) decreased with cell age, it was not tightly coupled to the S phase. (unibas.ch)
The low Pol II binding at the NDRG1 promoter in SW620 was associated with gene-wide decrease in histone H4 acetylation and increase in histone H3 serine 10 phosphorylation. (nih.gov)
In conclusion we observed unique histone modifications in two colon cancer cell lines with different metastatic potentials, indicating possible mechanisms for the down-regulation of NDRG1 in metastatic SW620. (nih.gov)
The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. (cancerindex.org)
A: Schematic of DNA methylation and histone modifications in neural stem cells (NSCs). (wjgnet.com)
3,3'-Diindolylmethane, but not indole-3-carbinol, inhibits histone deacetylase activity in prostate cancer cells. (oregonstate.edu)

Posttranscriptional regulation of GAP-43 gene expression in PC12 cells through protein kinase C-dependent stabilization of the mRNA. (rupress.org)
Regulation of gene expression, or gene regulation, includes a wide range of mechanisms that are used by cells to increase or decrease the production of specific gene products (protein or RNA). (wikipedia.org)
Virtually any step of gene expression can be modulated, from transcriptional initiation, to RNA processing, and to the post-translational modification of a protein. (wikipedia.org)
Gene regulation is essential for viruses, prokaryotes and eukaryotes as it increases the versatility and adaptability of an organism by allowing the cell to express protein when needed. (wikipedia.org)
Any step of gene expression may be modulated, from signaling to transcription to post-translational modification of a protein. (wikipedia.org)
These results indicate that Gc protein appears to be regulated differently than the other members of this gene family, albumin and alpha-fetoprotein (AFP), which are negative acute phase reactants. (nih.gov)
This was related to an increased DNMT3a protein association with a discreet region of the Nr3c1 (GR gene) promoter, shown here to undergo increased DNA methylation after FSS. (karger.com)
The differential accumulation of H5 reflected (i) an increase in the transcriptional activity of the H5 gene occurring at the erythroblast stage, (ii) an apparent longer half-life of H5 mRNA, and (iii) a higher stability of the protein. (unibas.ch)
We measured the levels of gene 5 and gene 6 mRNA and showed that they were not significantly different, and protein analysis indicated no difference in stability of NSP1 compared with VP6. (microbiologyresearch.org)
Nucleotide sequence and expression in Escherichia coli of the gene encoding the nonstructural protein NCVP2 of bovine rotavirus. (microbiologyresearch.org)
Cloning and nucleotide sequence of the simian rotavirus gene 6 that codes for the major inner capsid protein. (microbiologyresearch.org)
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. (nih.gov)
Co-staining for an epithelial cell marker identified most WUPyV viral protein 1-positive cells as respiratory epithelial cells. (cdc.gov)
Scope includes mutations and abnormal protein expression. (cancerindex.org)
The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. (cancerindex.org)
The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. (cancerindex.org)
What does this gene/protein do? (cancerindex.org)
RB1 encodes the protein pRB and was the first tumor suppressor gene to be molecularly defined. (medscape.com)
PTEN encodes a protein kinase of the same name and functions as a tumor suppressor through regulation of cell proliferation. (medscape.com)
We also identified 24 apaQTLs associated with protein levels, but not mRNA expression. (elifesciences.org)
Thus, our work demonstrates that APA links genetic variation to variation in gene expression, protein expression, and disease risk, and reveals uncharted modes of genetic regulation. (elifesciences.org)
These defects are mediated by increased expression of a protein called Xrp1, which is involved in gene expression regulation. (europa.eu)
A very important finding reported in this paper is that ALS-mutant FUS protein is toxic to both skeletal muscle cells and motor neurons. (europa.eu)
Xrp1 is a DNA-binding protein that is involved in gene expression regulation. (europa.eu)
Approximately 80% of the human genome contains functional DNA, including protein coding genes, non-protein coding regulatory DNA elements and non-coding RNAs (ncRNAs). (intechopen.com)
Post-transcriptional events play a major role in determining this signature, which is evidenced by the fact that alternative RNA splicing takes place in more than half of the human genes, and, among protein coding genes, more than 60% contain at least one conserved miRNA-binding site. (intechopen.com)
Differential expression of polycytosine-binding protein isoforms in adrenal gland, locus coeruleus and midbrain. (rochester.edu)
The course is focused on principles and mechanisms of gene expression regulation in both prokaryotic and eukaryotic cells, including regulation of transcription, translation and protein stability. (cuni.cz)
The molecular mechanisms of other cellular processes like protein sorting and distribution, membrane transport, cytoskelet function and basic processes of cell signalling are also emphasised. (cuni.cz)
Their regulation may be carried out either through direct binding to DNA as peroxisome proliferator-activated receptors or via modulation in an indirect manner of signaling pathway molecules (e.g., protein kinase C) and other transcription factors (nuclear factor kappa B and sterol regulatory element binding protein). (springer.com)
Lipid bilayer regulation of membrane protein function: gramicidin channels as molecular force probes. (springer.com)
MBD5 gene mutations and deletions prevent one copy of the MBD5 gene in each cell from producing any functional protein, which reduces the total amount of this protein in cells. (medlineplus.gov)
An increase or decrease in MBD5 protein disrupts gene expression that is normally well-controlled by this protein, which is likely why duplications and deletions involving the MBD5 gene lead to the same signs and symptoms. (medlineplus.gov)
All the fluorinated compounds are unable to transfect RD-4 cells, if used alone, but they are all able to deliver a plasmid carrying an enhanced green fluorescent protein (EGFP) expression cassette, when co-formulated with 1,2-dioleyl-sn-glycero-3-phosphoethanolamine (DOPE) in a 1:2 ratio. (bvsalud.org)
protein sorting and protein maturation by passage through the cytoplasmic organelles of the cell. (lu.se)
[ 1 ] His prediction was subsequently supported by the cloning of the retinoblastoma tumor suppressor gene ( RB1 ) and by functional studies of the retinoblastoma protein, Rb. (medscape.com)
For example, the TP53 gene, located on chromosome 17, encodes a 53-kd nuclear protein that functions as a cell cycle checkpoint. (medscape.com)
The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)
The p19ARF protein, which is encoded by the same locus as p16, also leads to cell cycle arrest by inhibiting the ability of MDM2 to inactivate TP53. (medscape.com)
The p16INK4A protein is a cell-cycle inhibitor that acts by inhibiting activated cyclin D:CDK4/6 complexes, which play a crucial role in the control of the cell cycle by phosphorylating Rb protein. (medscape.com)
There will be involved gene engineering and molecular biology methods such as PCR, SDS-PAGE, qPCR, western blot, DNA-protein interaction assays, flow cytometry etc. (lu.se)
2022 ) Protein domain-dependent vesiculation of Lipoprotein A, a protein that is important in cell wall synthesis and fitness of the human respiratory pathogen Haemophilus influenzae . (lu.se)

Knockdown of NDRG1 in SW480 to a level that is similar to that in SW620 also modulated cell cycle and proliferation in SW480 towards the status of the highly metastatic SW620. (nih.gov)
At a fundamental level, cancer is caused by an accumulation of genetic changes that result in unregulated cell growth and proliferation. (medscape.com)
The rate of ribosome production has far-reaching influence on the fate of the cell, and dictates its size, proliferation, and ability to translate global or specific mRNAs. (upenn.edu)
The atherosclerotic process is accelerated by a myriad of factors, such as the release of inflammatory chemokines and cytokines, the generation of reactive oxygen species (ROS), growth factors, and the proliferation of vascular smooth muscle cells. (hindawi.com)
Changes in dietary fatty acids, specifically the polyunsaturated fatty acids of the ω-3 and ω-6 families and some derived eicosanoids from lipoxygenases, cyclooxygenases, and cytochrome P-450, seem to control the activity of transcription factor families involved in cancer cell proliferation or cell death. (springer.com)
Regulation of global gene expression and cell proliferation by APP. (bvsalud.org)
Results from MTT proliferation assay, electrophoresis mobility shift assay (EMSA), transient transfection assay tests and atomic force microscopy (AFM) imaging confirm that pyridinium gemini surfactants could be a valuable tool for gene delivery purposes, but their performance is highly dependent on the spacer length and strictly related to their structure in solution. (bvsalud.org)
Inactivation of tumor suppressor genes, whose products normally provide negative control of cell proliferation, contributes to malignant transformation in various cell types. (medscape.com)

We have previously shown that nerve growth factor (NGF) selectively stabilizes the GAP-43 mRNA in PC12 cells. (rupress.org)
In contrast, the rates of degradation and the levels of the GAP-43 mRNA in control and TPA-treated cells were not affected by cycloheximide treatment. (rupress.org)
In conclusion, these data suggest that PKC activity regulates the levels of the GAP-43 mRNA in PC12 cells through a novel, translation-independent mRNA stabilization mechanism. (rupress.org)
FSS also caused a time-dependent increase in the expression of miR-124a, a microRNA known to reduce GR mRNA expression, which was inversely correlated with a reduction in GR mRNA levels 30 min after FSS. (karger.com)
Acute stress results in decreased GR mRNA expression specifically in the dentate gyrus. (karger.com)
On the other hand, the mRNA levels for these histones were tightly regulated during the cell cycle. (unibas.ch)
Although the transcription rates of the H5 and beta A-globin genes were comparable, differential accumulation of beta A-globin mRNA led to a 30- to 170-fold-higher copy number of the beta A-globin mRNA as the cell matured. (unibas.ch)
Polyribosome analysis showed that the majority of gene 6 mRNA was present on large polysomes. (microbiologyresearch.org)
In contrast, sedimentation of more than half of the gene 5 mRNA was subpolysomal. (microbiologyresearch.org)
The change in distribution of gene 5 mRNA in polyribosome gradients in response to treatment with low concentrations of cycloheximide suggested that gene 5 is a poor translation initiation template compared with gene 6 mRNA. (microbiologyresearch.org)
We have studied the effects of excitatory amino acids on the expression of the c-fos and c-jun mRNA in rat C6 glioma cells. (kyobobook.co.kr)
Expression of γ-globin mRNA was examined by primer extension, and transcriptional activity of the γ-globin gene promoter was determined by reporter gene assays. (elsevierpure.com)
Activation of the cAMP-dependent pathway by forskolin inhibited hemin-induced expression of γ-globin mRNA and decreased transcriptional activity of the γ-globin gene promoter. (elsevierpure.com)
To begin addressing this gap, we used 3' Seq to study the impact of genetic variation on alternative polyadenylation (APA) in the nuclear and total mRNA fractions of 52 HapMap Yoruba human lymphoblastoid cell lines. (elifesciences.org)
These observations suggest genetic variants can decrease mRNA expression levels by increasing usage of intronic PAS. (elifesciences.org)
Evidence for regulation of tyrosine hydroxylase mRNA translation by stress in rat adrenal medulla. (rochester.edu)

Such modifications are considered to be responsible for more or less permanent changes in gene expression levels. (wikipedia.org)
ligand-to-target signaling path visualization and assessment of how well prioritized ligands explain changes in gene expression in the receiver cell. (nature.com)
These changes in gene expression alter cell metabolism, tissue re-modeling, and even organismal responses such as increases in heart rate and ventilation. (nobelprize.org)
Interestingly, FLU-exposed biofilms showed no significant changes in gene expression over the course of the experiment. (who.int)

Detailed investigation of a few specialized mammalian genes, including the genes controlling the synthesis of hemoglobin, provide important models to understand how such long-range regulatory elements act. (ox.ac.uk)

On the other hand, chromatin immunoprecipitation showed a significantly higher level of RNA polymerase II (Pol II) association with the NDRG1 promoter in SW480 compared to SW620, in agreement with its gene expression level. (nih.gov)
Characterisation of the chromatin structures and dynamic mechanisms in human and murine cells and as well in the Malaria causing parasite Plasmodium falciparum. (uni-regensburg.de)
The phytochemical 3,3'-diindolylmethane decreases expression of AR-controlled DNA damage repair genes through repressive chromatin modifications and is associated with DNA damage in prostate cancer cells. (oregonstate.edu)
We used HiChIP, an assay which combines genome-wide chromatin conformation capture followed by deep sequencing (Hi-C) and chromatin immunoprecipitation (ChIP), to interrogate the EBV episome 3D conformation in different cancer cell lines. (bvsalud.org)
Molecular mechanisms for regulation of gene expression at different levels: remodeling of chromatin, initiation of transcription, nuclear transport and signalling, and RNA interference. (lu.se)

Hence these modifications may up or down regulate the expression of a gene. (wikipedia.org)
Some of these modifications that regulate gene expression are inheritable and are referred to as epigenetic regulation. (wikipedia.org)
The molecular steps in the replication pathway that regulate the levels of viral gene expression are not well defined. (microbiologyresearch.org)
Fig. 2: NicheNet prioritizes CAF-ligands based on their potential to regulate p-EMT genes in adjacent malignant cells in HNSCC. (nature.com)
Mitra A, Vo L, Soukar I, Chaubal A, Greenberg ML, Pile LA. Isoforms of the transcriptional cofactor SIN3 differentially regulate genes necessary for energy metabolism and cell survival. (wayne.edu)
The Paralkar Lab has identified that key hematopoietic and leukemic transcription factors bind to rDNA and regulate rRNA transcription, and we are interested in understanding how the binding of cell-type-specific transcription factors regulates the activity of Polymerase I and the transcription of rRNA in normal hematopoiesis, and how this regulation is co-opted in leukemia to drive abundant ribosome biogenesis. (upenn.edu)
Mechanisms that regulate development from single cell to multicellular organisms. (lu.se)

This disorder is caused by dominant-negative mutations in the STAT3 gene ( 9 ). (cdc.gov)
SIN3 is required for viability of multi-cellular organisms and mutations in components in the complex have been linked to defects in cell cycle progression. (wayne.edu)
[ 3 ] Loss of function mutations in this gene are implicated in pediatric retinoblastoma. (medscape.com)
This paper reports that loss of function of the Drosophila gene cabeza (the Drosophila equivalent of the human FUS gene, mutations in which can cause a familial form of the motor neurodegenerative disease amyotrophic lateral sclerosis or ALS) induces muscle developmental defects. (europa.eu)
Less frequently, MAND is caused by mutations that affect only the MBD5 gene. (medlineplus.gov)
Because mutations in the MBD5 gene and changes on chromosome 2 that involve this gene both cause MAND, researchers believe that MBD5 gene changes underlie most of the signs and symptoms of the condition. (medlineplus.gov)
Splicing factor mutations are particularly prevalent in MDS, a group of heterogeneous hematological disorders characterized by defective blood stem cells and a high risk of leukemia development. (lu.se)

CD90+ prostate tumor-associated stromal cells differed from their normal counterpart in expression of multiple genes, some of which are potentially involved in organ development. (nih.gov)
Finally, we identified aberrant methylation in multiple genes also targeted by recurring DNA copy number alterations in ALL, suggesting that these genes are inactivated far more frequently than suggested by structural genomic analyses alone. (lu.se)
A strong enhancer was located near the BILF2 gene and looped to multiple genes around BALFs loci. (bvsalud.org)

Transcription of a gene by RNA polymerase can be regulated by several mechanisms. (wikipedia.org)
We have investigated potential mechanisms of regulation of rotavirus gene expression by functional evaluation of two differentially expressed viral mRNAs. (microbiologyresearch.org)
The objective of this study was to investigate the potential mechanisms involved in silencing of the NDRG1 gene. (nih.gov)
SW480 and SW620 are two colon cancer cell lines established from the same patient with different metastatic potentials, making them an ideal model for investigation of metastatic mechanisms. (nih.gov)
There are a limited number of mechanisms that enable dynamic regulation in yeast, and fewer still that are appropriate for application in an industrial setting. (biomedcentral.com)
Microorganisms possess a variety of mechanisms for modulating gene expression levels according to changes in their environment. (biomedcentral.com)
The objective being to give an overview of the methods used in the discovery of molecular mechanisms used by model bacteria and the application of this in understanding the basic processes involved in bacterial growth and development in terms of regulation and biological function. (ncl.ac.uk)
but the molecular mechanisms underlying how cells adapt to variations in oxygen supply were unknown until the prize-winning work described here. (nobelprize.org)
I branched to include more genes as targets, and more diseases and pharmaceuticals or everyday actives, to understand the mechanisms of effects. (fdu.edu)
of the most critical events which lead to tissue patterning involves mechanisms going beyond single cells. (lu.se)
This article reviews the current knowledge and experimental research about the mechanisms by which fatty acids and their derivatives control specific gene expression involved during carcinogenesis. (springer.com)
Knowledge of the mechanisms by which fatty acids control specific gene expression may identify important risk factors for cancer and provide insight into the development of new therapeutic strategies for a better management of whole body lipid metabolism. (springer.com)
Mechanisms for maintaining genetic information during cell division and the generation of genetic variation: replication, mitosis, meiosis, recombination. (lu.se)

Research in the Paralkar Lab spans the spectrum from human patient sample studies and mouse models to cutting-edge molecular biology tools, high-throughput sequencing approaches, and novel computational algorithms, all with the goal of gaining insight into how the transcription of coding genes and noncoding ribosomal DNA genes is regulated in hematopoietic stem cells, myeloid progenitors, and in leukemia. (upenn.edu)
In a study published in the Molecular Cell journal, they explain what makes hematopoietic stem cells acquire malignant traits in cancer. (lu.se)

Isolation and characterization of these stromal cells could provide valuable insight into the biology of the tumor microenvironment. (nih.gov)
Sophisticated programs of gene expression are widely observed in biology, for example to trigger developmental pathways, respond to environmental stimuli, or adapt to new food sources. (wikipedia.org)
Engineering dynamic, environmentally- and temporally-responsive control of gene expression is one of the principle objectives in the field of synthetic biology. (biomedcentral.com)
This module provides an up to date understanding of how fundamental research in bacterial cell biology helps to elucidate central biological questions such as the control and regulation of cell division and of gene expression in bacteria. (ncl.ac.uk)
The lecture sequence will comprise of a module introduction followed by lectures on specific aspects of bacterial cell biology. (ncl.ac.uk)
Interest in an undergraduate biochemistry course led me to a Ph.D. program in Biochemistry and Molecular Biology where I researched transcriptional regulation of type I collagen gene in cells from hypertensive fibrotic animal models. (fdu.edu)
My central interest is molecular cell biology. (fdu.edu)
Journal of Cell Biology 2018, 217(11):3947-3964. (europa.eu)
Molecular Cell Biology (Lodish et al. (cuni.cz)
Molecular biology of the Cell (Alberts et al. (cuni.cz)
Molecular Biology of the Gene (Watson et al. (cuni.cz)
BMC Cell Biology, 9 , 45. (springer.com)
Gene regulation in developmental biology and the cell cycle. (lu.se)

Identification, synthesis, and modifications of simian rotavirus SA11 polypeptides in infected cells. (microbiologyresearch.org)
rRNA transcription rates vary dramatically between different normal cell types in the hematopoietic tree, and leukemic cells have characteristic prominent nucleoli, indicating robust ribosome synthesis. (upenn.edu)
Thus, enhanced extracellular matrix or beta-glucan synthesis during biofilm growth might prevent antifungals, such as azoles and polyenes, from reaching biofilm cells, thus limiting their toxicity to these cells and the associated transcriptional responses. (who.int)

Due to the risk of adverse or immunogenic reaction or replication connected with the more efficient viral vectors, non-viral vectors are in many cases considered as a preferred strategy for gene delivery into eukaryotic cells. (bvsalud.org)
You will also employ functional assays investigating host-pathogen interactions in vitro, such as serum bactericidal assay or adherence to eukaryotic cells. (lu.se)

Montalbán-Loro R, Domingo-Muelas A, Bizy A, Ferrón SR. Epigenetic regulation of stemness maintenance in the neurogenic niches. (wjgnet.com)
Figure 2 Epigenetic regulation of gene expression. (wjgnet.com)
2011. Dietary factors and epigenetic regulation for prostate cancer prevention. . (oregonstate.edu)

It is also unknown whether the loss or gain of other genes in chromosome 2 deletions or duplications contributes to the variable features of MAND. (medlineplus.gov)

Repressors bind to the Operator, coding sequences on the DNA strand that are close to or overlapping the promoter region, impeding RNA polymerase's progress along the strand, thus impeding the expression of the gene. (wikipedia.org)
In turn, we have addressed how, during this process, the upstream elements control the correct spatial and temporal expression from the alpha-gene promoter which lies approximately 60 kb downstream of these elements. (ox.ac.uk)
The silencing of NDRG1 in SW620 was not due to promoter hyper-methylation as bisulfite sequencing of the NDRG1 promoter showed minimal DNA methylation in both cell lines. (nih.gov)
The ability to fine-tune the timing and population density at which gene expression is activated from the SUC2 promoter was demonstrated by varying the ratio of glucose to sucrose in the growth medium. (biomedcentral.com)
The low noise levels and high dynamic range of the SUC2 promoter make it a promising option for implementing dynamic regulation in yeast. (biomedcentral.com)
Effects of sulforaphane and 3,3'-diindolylmethane on genome-wide promoter methylation in normal prostate epithelial cells and prostate cancer cells. (oregonstate.edu)
The viral C promoter regulates the expression of all EBV nuclear antigens (EBNAs), some of which are very far away from the C promoter. (bvsalud.org)
Methods for analysis of gene expression: microarray, hybridisation, promoter analyses. (lu.se)

In an EBV-transformed lymphoblastoid cell line (LCL) GM12878 expressing type III EBV latency genes, abundant genomic interactions were identified by H3K27ac HiChIP. (bvsalud.org)

William Kaelin, Jr. was in 1995 engaged in the study of the von Hippel-Lindau tumor suppressor gene, and after isolation of the first full-length clone of the gene showed that it could suppress tumor growth in VHL mutant tumorigenic cell lines. (nobelprize.org)

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. (cancerindex.org)

These data define a regulatory mechanism for the difference in amounts of VP6 and NSP1 and provide evidence for post-transcriptional control of rotavirus gene expression mediated by the translational efficiency of individual viral mRNAs. (microbiologyresearch.org)

The ability to engineer dynamic regulation is highly desirable in microbial cell factories because many of the most productive genetic modifications used for optimising engineering objectives are also detrimental to cell growth and survival. (biomedcentral.com)
We utilize a combination of biochemical, molecular and genetic techniques to understand the mechanism of SIN3 gene regulation. (wayne.edu)
We found that the different genetic subtypes of ALL are characterized by distinct DNA methylation signatures that exhibit significant correlation with gene expression profiles. (lu.se)
The Paralkar Lab has developed customized genomes and computational pipelines to map datasets to rDNA, and we are interested in developing advanced tools to map and interpret the genetic and epigenetic profiles of rDNA in normal and malignant cells. (upenn.edu)
The genetic material must be delivered inside the cells by means of vectors. (bvsalud.org)
Gene technology: basic and applied molecular genetic methods. (lu.se)
A team of researchers led by Dr. Cristian Bellodi recently discovered a hardwired genetic control mechanism modulating individual spliceosomal components, known as splicing factors, in cells harboring oncogenic lesions common in human cancers. (lu.se)

It specifically negatively regulates the PI3K-AKT signaling pathway to induce cell cycle arrest. (medscape.com)
Perturbation of the BILF2 enhancer by CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) altered the expression of BILF2 enhancer-linked genes, including BARF0 and BALF2, suggesting that this enhancer regulates the expression of linked genes. (bvsalud.org)

This research led me to post-doctoral fellowships predominantly on the extracellular matrix genes methylation, transcription, cloning, and photomedicine at Boston University Medical Center, Massachusetts General Hospital, and NYU Medical Center. (fdu.edu)
In this regard, our study led us to observe that AMB physically bound C. albicans biofilms and beta-glucans, which have been proposed to be major constituents of the biofilm extracellular matrix and to prevent azoles from reaching biofilm cells. (who.int)

This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. (cancerindex.org)
membranous co-expression of CADM1 and LATS2 in lung tumors predicts good prognosis. (cancerindex.org)
Cell adhesion molecule-1 (CADM1) is a member of the immunoglobulin superfamily that functions as a tumor suppressor of lung tumors. (cancerindex.org)
Glioblastomas are intrinsic brain tumors believed to originate from neuroglial stem or progenitor cells. (frontiersin.org)
These data provided new insight into the regulation of EBV latency gene expression in different EBV-associated tumors. (bvsalud.org)
Although carcinogenic roles for the INK4B, INK4C, INK4D, CIP1, KIP1, and KIP2 genes appear to be limited, INK4A is among the most commonly mutated genes in human tumors. (medscape.com)

Effect of dietary fatty acids on inflammatory gene expression in healthy humans. (springer.com)

In addition, these alterations affect 3 principal categories of genes, as follows: proto-oncogenes, tumor suppressor genes, and DNA repair genes. (medscape.com)

Studies have suggested that statins may inhibit tumor cell growth and possibly prevent carcinogenesis. (cdc.gov)

The data source networks, NicheNet's ligand-target matrix and the processed ligand treatment and case study expression datasets are available on Zenodo ( https://doi.org/10.5281/zenodo.3260758 ) 59 . (nature.com)
Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancerindex.org)
Given that rRNA accounts for the bulk of the transcriptional output of the cell, the inability to map datasets to rDNA has historically been a major limitation, and has created a significant knowledge gap in our understanding of the most abundant RNA in the cell. (upenn.edu)

DNA methylation is the longest appreciated epigenetic modification and has been accepted to play a critical role in maintaining euchromatin and silencing genes. (intechopen.com)

In addition, it can stimulate apoptosis of cells containing damaged DNA. (medscape.com)
The TP53 gene is also capable of stimulating apoptosis of cells containing damaged DNA. (medscape.com)

Epigenetics describes modifications that affect gene expression that are not encoded within the DNA sequence. (intechopen.com)

Often, one gene regulator controls another, and so on, in a gene regulatory network. (wikipedia.org)
Nucleus-specific expression in the multinuclear mushroom-forming fungus reveals different nuclear regulatory programs. (broadinstitute.org)
In general, these elements contain a high density of evolutionarily conserved, transcription factor-binding sites and in many ways resemble the upstream regulatory elements found adjacent to the promoters of genes in simpler organisms, differing only in the distance over which they act. (ox.ac.uk)
We present NicheNet ( https://github.com/saeyslab/nichenetr ), a method that predicts ligand-target links between interacting cells by combining their expression data with prior knowledge on signaling and gene regulatory networks. (nature.com)
We applied NicheNet to tumor and immune cell microenvironment data and demonstrate that NicheNet can infer active ligands and their gene regulatory effects on interacting cells. (nature.com)
Databases used to create the NicheNet model are mentioned and referred to in the Methods section Collection of ligand-receptor, intracellular signaling and gene regulatory interactions , in Supplementary Table 1 and in the Supplementary Notes . (nature.com)
Current objectives of the lab are to understand regulatory pathways that affect SIN3 activity and to understand the consequences of SIN3 recruitment at target genes. (wayne.edu)
pRB functions as a negative regulatory transcription factor during the G1 to S phase cell cycle transition. (medscape.com)
Transcription Factors, Gene Regulatory Networks, and Agronomic Traits. (routledge.com)

Prostate CD90+ stromal fibromuscular cells from tumor specimens were isolated by cell-sorting and analyzed by DNA microarray. (nih.gov)
We analyzed 137 B-lineage and 30 T-lineage childhood ALL cases using microarray analysis of DNA copy number alterations and gene expression, and genome-wide cytosine methylation profiling using the HpaII tiny fragment enrichment by ligation-mediated PCR (HELP) assay. (lu.se)

Comparative genomics of white and opaque cell states supports an epigenetic mechanism of phenotypic switching in Candida albicans. (broadinstitute.org)
High concentrations of fermentable carbon sources repress gene expression from galactose promoters (GAL) via a carbon catabolite repression mechanism [ 7 ], and galactose utilisation genes are not activated during growth on sucrose [ 8 ]. (biomedcentral.com)
Tanay, A. & Regev, A. Scaling single-cell genomics from phenomenology to mechanism. (nature.com)
Mechanism of neurotrophic action of nobiletin in PC12D cells. (rochester.edu)
2017. Rapamycin inhibits the secretory phenotype of senescent cells by a Nrf2-independent mechanism. . (oregonstate.edu)

NRG1α was detected in serous cells within normal submucosal glands and in epithelial cells lining honeycombing areas in IPF, and was not detected in other patients. (bmj.com)
Conclusion Expression of SOX2 and MUC5B and lack of SPDEF in atypically differentiated cells of bronchiolised distal airspaces are consistent with abnormal programming of airway epithelial cells in IPF. (bmj.com)

Other differential expression between the stromal cell types included that of the CXC-chemokine genes. (nih.gov)
cAMP and cGMP may have differential roles in the regulation of γ-globin gene expression in erythroid cells. (elsevierpure.com)

The expression of the genes for several histones and beta A-globin was examined in the chicken erythroid cells lineage. (unibas.ch)

We herein demonstrated that CADM1 interacts with Hippo pathway core kinases and enhances the phosphorylation of YAP1, and also that the membranous co-expression of CADM1 and LATS2 predicts a favorable prognosis in lung adenocarcinoma. (cancerindex.org)

The aim of this study was to examine the effects of 1, 10, 40, or 80 microg MWCNT administered by pharyngeal aspiration on bronchoalveolar lavage (BAL) fluid for polymorphonuclear cell (PMN) infiltration, lactate dehydrogenase (LDH) activity, and lung histopathology for inflammatory and fibrotic responses in mouse lungs 1 mo, 6 mo, and 1 yr postexposure. (cdc.gov)
A splice site variant in the bovine RNF11 gene compromises growth and regulation of the inflammatory response. (123dok.net)

not unique for the embryonic system but are shared by other ogenesis regulation must consider different scales of multi-cellular modeling areas. (lu.se)

Animal cells undergo fundamental shifts in gene expression when there are changes in the oxygen levels around them. (nobelprize.org)

Posttranscriptional regulation of adrenal TH gene expression contributes to the maladaptive responses triggered by insulin-induced recurrent hypoglycemia. (rochester.edu)

NCBI Gene Expression Omnibus, GSE138197. (elifesciences.org)

2022 ) Gene Expression Regulation in Airway Pathogens: Importance for Otitis Media. (lu.se)

Interest in signal transduction and hormonal regulation of genes made me adopt an endocrinology course and make it into molecular endocrinology. (fdu.edu)

2017. A functional pseudogene, NMRAL2P, is regulated by Nrf2 and serves as a coactivator of NQO1 in sulforaphane-treated colon cancer cells. . (oregonstate.edu)
CRISPRi also validated the functional connection between BILF2 enhancer and BARF1 gene. (bvsalud.org)

Fetal hemoglobin inducers such as hemin, butyrate, and hydroxyurea stimulate γ-globin gene expression by activating the cyclic GMP (cGMP)-dependent pathway. (elsevierpure.com)
Although cGMP activates the cyclic AMP (cAMP)-dependent pathway by suppressing cGMP-inhibited phosphodiesterase 3 (PDE3), the effects of the cAMP-dependent pathway on γ-globin gene expression are unknown. (elsevierpure.com)
The cAMP-dependent pathway was activated in K562 cells using the adenylate cyclase activator forskolin. (elsevierpure.com)
The cAMP-dependent pathway was found to be activated in K562 cells in which the cGMP-dependent pathway was activated by hemin. (elsevierpure.com)
These results suggested that the cAMP-dependent pathway, which is independent of MAPK pathways, plays a negative role in γ-globin gene expression in K562 cells. (elsevierpure.com)
We show that dysregulation of appropriate expression of components of the fibroblast growth factor (FGF) pathway contributes to muscle developmental defects in cabeza mutant Drosophila. (europa.eu)

Normal hematopoiesis requires an intricate balance in the bone marrow between the ability of stem cells to maintain themselves for decades of life while producing billions of mature blood cells every day. (upenn.edu)
and stem cell regeneration. (lu.se)
Get ready for an exciting start to our 2024 Stem Cell Stars series! (lu.se)
What makes blood stem cells transform? (lu.se)

In addition, we provide tutorials on model validation (evaluation of target gene and ligand activity), model construction and parameter optimization such that users can benchmark their methods against NicheNet and construct their own models (for example, with their own data sources to include more cell type specificity). (nature.com)

Ident- cult to deduce interaction rules from observations of the ified transcription factors for specific cell fates are, for entire system. (lu.se)

Principles for regulation of gene expression through intercellular signalling. (lu.se)

Computational methods that model how gene expression of a cell is influenced by interacting cells are lacking. (nature.com)

We also identified an epigenetic signature common to all cases, with correlation to gene expression in 65% of these genes, suggesting that a core set of epigenetically deregulated genes is central to the initiation or maintenance of lymphoid transformation. (lu.se)

Anatomy29

Organisms13

Diseases5

Chemicals and Drugs50

Analytical, Diagnostic and Therapeutic Techniques and Equipment33

Phenomena and Processes78

Disciplines and Occupations3

Information Science6

Health Care3

Proteins13

Differentiation17

Histone9

Protein42

Proliferation8

MRNA16

Changes in gene expression4

Mammalian1

Chromatin5

Regulate7

Mutations7

Multiple genes3

Mechanisms13

Hematopoietic stem2

Biology13

Synthesis3

Eukaryotic cells2

Epigenetic regulation3

Chromosome1

Promoter8

Genomic1

Tumor supp1

Multicellular1

Post-transcriptional control1

Genetic7

Regulates2

Extracellular matrix2

Tumors6

Humans1

Repair genes1

Carcinogenesis1

Datasets3

Euchromatin1

Apoptosis of cells contain2

Modifications1

Regulatory9

Microarray2

Mechanism5

Epithelial2

Differential2

Histones1

Phosphorylation1

Inflammatory2

Embryonic1

Undergo1

Posttranscriptional1

NCBI1

20221

Hormonal1

Functional2

Pathway6

Stem4

Target gene1

Transcription factors1

Intercellular1

Computational1

Initiation1