• vitro
  • This study provides an extraordinary visualization of cleavage furrow and aster formation and, for the first time, reconstitutes this process in an in vitro experimental system. (xenbase.org)
  • A cell-free extract of these oocytes catalyzes apparent disassembly of purified Drosophila nuclei as well as of nuclear lamin polymers formed in vitro from isolated interphase lamins. (biologists.org)
  • We have previously described a cell-free recombination system derived from hamster cell nuclear extracts in which the in vitro recombination between a hamster preinsertion sequence, the cloned 1768 base-pair p7 fragment, and adenovirus type 12 (Ad12) DNA has been demonstrated. (uni-muenchen.de)
  • We have developed a cell-free in vitro assay for β-catenin signaling activity that utilizes transcriptionally active nuclei and cytoplasm from cleavage-blocked Xenopus laevis embryos. (rupress.org)
  • Systems biocatalysis is an approach consisting of organizing enzymes in vitro to generate an artificial metabolism for synthetic purposes following the identification of a reaction sequence catalysed by specific enzymes, construction of a controllable metabolic pathway and process intensification for the effective production of the products. (teknoscienze.com)
  • In vitro translation (IVT) systems have been used for rapid expression of functional proteins, including enzymes. (thermofisher.com)
  • Alternatively, the transcription can be performed separately using in vitro transcription system (Promega): mRNA is transcribed according to manufacturer instructions with T7 RiboMAX in vitro transcription kit and purified thereafter with total RNA isolation kit. (scienceexchange.com)
  • progenitor
  • 2 ] presented a CRISPER/Cas9 gene-editing system with homologous recombination at the HBB gene in hematopoietic stem cells and achieved 90% targeted integration of purified hematopoietic stem and progenitor cells. (springer.com)
  • These regulatory mechanisms seem to promote the formation of relatively "open" and "poised" epigenetic states permissive for transcriptional activity that are associated with multi-lineage potential in stem and progenitor cells and to mediate the execution of lineage-specific gene expression programs, including the silencing of genes associated with alternate cell fates, in more mature cellular species. (mdpi.com)
  • tumor cells
  • We believe that better understanding how epigenetic mechanisms operate in neural cells and identifying the etiologies and consequences of epigenetic deregulation in tumor cells and CSCs, in particular, are likely to promote the development of enhanced molecular diagnostics and more targeted and effective therapeutic agents for treating recalcitrant nervous system tumors. (mdpi.com)
  • extract
  • We developed a cell-free system to recapitulate cytokinesis signaling using cytoplasmic extract from Xenopus eggs. (xenbase.org)
  • The aim of this study was to investigate the antioxidant activity of Ixora parviflora extract (IPE) in a cell-free system and erythrocytes, and the ability of IPE to inhibit reactive oxygen species (ROS) generation in human fibroblasts (Hs68) after ultraviolet (UV) exposure. (mdpi.com)
  • Publications] Someya, A.: 'The association and involvement of the P1 protein family in a cell free extract of Xenopus eggs' Biochem.Biophys.Res.Commun.209. (nii.ac.jp)
  • small molecule
  • These results suggest that one can not only rapidly express highly active caspase-3 using human IVT system but also utilize this system to carry out small molecule inhibitor screens to identify caspase-3 modulators. (thermofisher.com)
  • The identification of the small molecule, pharmacological inhibitor system in stem cells, led by Ali Brivanlou, Ph.D., head of the laboratory of molecular vertebrate embryology and his research associate, Noburo Sato, Ph.D., so far demonstrates superior stability over other methods designed to circumvent the need for mouse feeder cells. (innovations-report.com)
  • fetal
  • High-quality Tet System Approved Fetal Bovine Serum for cell culture has been functionally tested * to ensure that it permits the full range of tetracycline-regulated induction with well-characterized Tet cell lines. (clontech.com)
  • Because Clontech's Tet-On and Tet-Off Systems are extremely sensitive, even "antibiotic-free" fetal bovine serum that has no negative effect on cell growth may still exhibit enough activity to alter Tet-regulated gene expression. (clontech.com)
  • Testing for the presence of tetracyclines in fetal or calf serum is not equivalent to the highly sensitive functional testing that is performed on Clontech Tet System Approved FBS. (clontech.com)
  • The only fetal bovine serum that is functionally tested with Clontech's tetracycline inducible expression systems. (clontech.com)
  • Finally, to confirm the ability of our ESC lines to provide the full range of differentiated tissue types, the stem cells were induced to develop into each of the three fetal germ layers. (biomedcentral.com)
  • By sorting fetal human pancreatic α, β, and δ cells by staining for certain hormones, Blodgett et al. (springer.com)
  • bacterial
  • Nod2 was reported to be oligomerized with adaptor protein RICK (RIP2/RIPK2) and IKK complexes, which can activate NF- κ B by muramyl dipeptide (N-Acetylmuramyl-L-Alanyl-D-Isoglutamine: MDP), one of the components of bacterial cell-wall peptidoglycan, and is utilized as an immune-stimulatory adjuvant for vaccination and for developing antibodies [ 4 - 8 ]. (hindawi.com)
  • feeder cells
  • Their creation using mouse feeder cells, a specialized growth medium, allows scientists to study their basic characteristics, but ultimately the HESCs are too risky to develop in applied medicine because mouse-associated viruses possibly contaminate them. (innovations-report.com)
  • Now, Rockefeller University researchers in collaboration with two European scientists, have discovered a way around this problem: they've devised a system for maintaining existing or new human stem cell lines that excludes the need for troublesome mouse feeder cells. (innovations-report.com)
  • To evaluate embryonic stem cell (ESC) harvesting methods with an emphasis on derivation of ESC lines without feeder cells or sera. (biomedcentral.com)
  • Here we describe production of ESCs from blastocysts obtained from a standard mouse strain in the absence of feeder cells or sera, with an emphasis on a laser-based ICM isolation modality. (biomedcentral.com)
  • nuclear
  • However, the preincubation of cytosols from normal cells with the drug had marginal effects on the nuclear morphology. (aacrjournals.org)
  • DNA methylation, histone and chromatin modification, non-coding RNA deployment, RNA editing, and nuclear reorganization) and discuss the broad spectrum of epigenetic alterations that have been uncovered in pediatric and adult nervous system tumors. (mdpi.com)
  • inhibition
  • HN can be recognized by the host immune system and antibodies against epitopes within HN can neutralize its activity through the inhibition of the function and/or activity of either hemagglutinin or neuraminidase. (frontiersin.org)
  • Immunodepletion of DUF from the DNA synthetic system lead to inhibition of intiation of DNA replication. (nii.ac.jp)
  • Specific inhibition of cell free expressed caspase-3 using Ac-DEVD-CHO inhibitor. (thermofisher.com)
  • Chemical inhibition of GSK-3 thus mimics activation of Wnt and this keeps HESCs in an active, undifferentiated state - one of the crucial basic qualities of embryonic stem cells. (innovations-report.com)
  • pluripotency
  • This new system for maintaining pluripotency could be a providential break for basic researchers and clinicians investigating the potential of HESCs, as it is a potential first step in providing an unlimited source of tissue transplant if HESCs' potential comes to fruition in clinical medicine. (innovations-report.com)
  • DPSCs were characterized first, followed by cell proliferation, pluripotency, and migration study in SCM and E8 medium. (springer.com)
  • Compared with serum-containing medium, E8 medium exhitibed higher ability in maintaining the cell proliferation, pluripotency, migration, and stability. (springer.com)
  • utilize
  • To reduce the cost and power consumption of such systems, it is important to utilize low-quality transceiver hardware at the APs. (diva-portal.org)
  • mechanisms
  • Further work will be required to ascertain that the cell-free recombination system mimics certain elements of the mechanisms of integrative recombination and to purify the cellular components essential for recombination. (uni-muenchen.de)
  • 3. Cell cycle study will expose the mechanisms leading to cancer, which has significant implication on cancer diagnosis and cancer treatment. (powershow.com)
  • Recent advances have begun to elucidate how epigenetic regulatory mechanisms are responsible for establishing and maintaining cell identity during development and adult life and how the disruption of these processes is, not surprisingly, one of the hallmarks of cancer. (mdpi.com)
  • Epigenetic mechanisms are deployed in response to complex profiles of cell-extrinsic, cell-cell, and cell-intrinsic signals and are responsible for the establishment, maintenance, and refinement of cell identity and function that occurs during development and adult life [ 1 ]. (mdpi.com)
  • assays
  • A Travelling Fellowship from Journal of Cell Science allowed her to spend time in Prof Maddy Parson's lab at King's College London, learning new cell migration assays and analysing fibroblasts cultured from individuals with Parkinson's. (biologists.org)
  • Cesare, G. Integrated System Based on Thin Film Technologies for Cell-Based Bioluminescence Assays. (mdpi.com)
  • Xenopus
  • In a new paper appearing in Science (10 October 2014, Science 346 (6206):244-247 ), Phuong Nguyen and Aaron Groen, along with their colleagues at Harvard Medical School (Boston, MA), have reconstituted the organization of cytokinesis signaling outside living cells, using a the cell-free Xenopus system. (xenbase.org)
  • We purified a novel protein (designated to DUF) which has an activity to untwist DNA and open double strand DNA to single strand DNA from cell-free DNA synthetic system of Xenopus eggs. (nii.ac.jp)
  • Publications] Someya, A.: 'The possible involvement of DNA-dependent protein kinase in the phosphorylation of P1 protein in cell-free DNA replication of Xenopus eggs' Biochem.Biophys.Res.Commun.213. (nii.ac.jp)
  • Publications] Someya, A., Shioda, M., Okuyama, A.: 'The association and involvement of some member of the P1 protein family in a cell-free DNA replication of Xenopus eggs. (nii.ac.jp)
  • chromatin
  • However, incubation of nuclei with cytosols from irofulven-treated CEM and LNCaP-Pro5 cancer cells resulted in characteristic apoptotic changes: partial chromatin condensation, nuclei breakage, and the release of small chromatin fragments. (aacrjournals.org)
  • cytotoxicity
  • The proposed lab-on-chip is particularly attractive for the development of portable cell-based biosensors useful for biological activity monitoring as well as for cell cytotoxicity evaluation. (mdpi.com)
  • stimulates
  • The 8-azido-ATP photolabeling system shows that GSSG stimulates ATP hydrolysis without affecting ATP binding, whereas GSH decreases ATP binding. (bu.edu)
  • activity
  • Chiang, H.-M. Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human Fibroblasts. (mdpi.com)
  • Wen K-C, Chiu H-H, Fan P-C, Chen C-W, Wu S-M, Chang J-H, Chiang H-M. Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human Fibroblasts. (mdpi.com)
  • The activity promoting cell-free recombination elutes from the Sephacryl S-300 matrix with the shoulder and not the peak fractions of the absorbancy profile. (uni-muenchen.de)
  • Here, we demonstrate the ability of this IVT system to rapidly express highly functional caspase-3 enzyme, as well as how it can be used to screen modulators of protein activity. (thermofisher.com)
  • The activity of poly(dA:dT) is tested using the A549-Dual™ reporter cell line. (invivogen.com)
  • This work presents a miniaturized lab-on-chip system suitable for monitoring the activity of living cells through the on-chip detection of their bioluminescence emission. (mdpi.com)
  • degradation
  • Taken together, these results suggest that activation of β-catenin signaling by lithium in this system may occur through a distinct activation mechanism that does not require modulation of levels through regulation of proteasomal degradation. (rupress.org)
  • sterile
  • Add 2.5 ng plasmid DNA to 9.5 µl aliquot of the 2x-master mix (thawed on ice) and adjust the final volume to 20.5 µl with sterile RNAse-free water. (scienceexchange.com)
  • 2 µl purified mRNA from one transcription reaction is added to 9.5 µl aliquot of the 2x-master mix and the final volume is adjusted to 20.5 µl with sterile RNAse-free water. (scienceexchange.com)
  • enzymes
  • There are also significant applications for expression systems in industrial fermentation , notably the production of biopharmaceuticals such as human insulin to treat diabetes , and to manufacture enzymes . (wikipedia.org)
  • lines
  • The patchy homologies are similar to those found earlier during the analyses of some of the junction sequences in integrated Ad12 genomes in Ad12-induced hamster tumor cell lines. (uni-muenchen.de)
  • In this study, the applicability of a cell-based sensor system using selected eukaryotic cell lines for the detection of aquatic pollutants is shown. (mdpi.com)
  • Insect or mammal cell lines are used when human-like splicing of mRNA is required. (wikipedia.org)
  • novel
  • Different tags were introduced flanking the ScFv800E6 backbone, with and without spacer arms, including a novel Strep II tag that outperforms conventional streptavidin-based detection systems. (youscribe.com)
  • Using a murine model, laser-assisted blastocyst dissection was performed and compared to conventional immunosurgery to assess a novel laser application for inner cell mass (ICM) isolation. (biomedcentral.com)