• In humans, professional antigen-presenting cells are specialized to present MHC class II peptides, whereas most nucleated somatic cells present MHC class I peptides. (wikipedia.org)
  • T cell epitopes presented by MHC class I molecules are typically peptides between 8 and 11 amino acids in length, whereas MHC class II molecules present longer peptides, 13-17 amino acids in length, and non-classical MHC molecules also present non-peptidic epitopes such as glycolipids. (wikipedia.org)
  • Likewise, a T cell propensity model has been proposed for identifying MHC binding peptides with amino acid compositions favoring T cell receptor interactions. (iedb.org)
  • Lastly, for each method, we calculate the percentage of known epitopes that rank within the 5% peptides with the highest predicted score. (biomedcentral.com)
  • In the large-scale benchmark calculation consisting of 216 known HIV epitopes covering all 12 recognized HLA supertypes, the NetCTL-1.2 method was shown to have a sensitivity among the 5% top-scoring peptides above 0.72. (biomedcentral.com)
  • To facilitate the discrimination, all nucleated cells present a selection of the peptides contained in their proteins on the cell surface in complex with Major Histocompatibility Complex class I (MHC class I) molecules. (biomedcentral.com)
  • Once inside the ER, the peptides may bind to MHC class I molecules, which are subsequently transported to the cell surface, where the complexes may be recognized by passing CTLs. (biomedcentral.com)
  • Reliable predictions of immunogenic peptides can minimize the experimental effort needed to identify new epitopes to be used in, for example, vaccine design or for diagnostic purposes. (biomedcentral.com)
  • The kinetics and products of degradation of HIV-1 Gag favored the production of peptides encompassing the immunodominant epitope and destruction of the subdominant one. (jci.org)
  • peptides bound to HLA-DR17 from bronchoalveolar lavage cells of sarcoidosis patients were analyzed in order to identify target antigens of the autoimmune response (see the related article beginning on page 3576). (jci.org)
  • the modulation of epitope immunodominance and the processing and presentation of HIV peptides for MHC class I recognition were shown to be dependent on flanking residues that were N terminal to the natural epitopes (see the related article beginning on page 3563). (jci.org)
  • Nishant Thakur, Abid Qureshi and Manoj Kumar : AVPpred: A software for the prediction of highly effective antiviral peptides. (imtech.res.in)
  • The candidate peptides used for this purpose must have several properties principally represented by their specificity and their location in the bacterial cell. (degruyter.com)
  • Besides the isolation and identification of major histocompatibility complex I-restricted peptides from the surface of cancer cells, one of the challenges is eliciting an effective antitumor CD8+ T-cell-mediated response as part of therapeutic cancer vaccine. (elifesciences.org)
  • Peptides were synthesized and tested for in vivo immunogenicity by screening peripheral blood mononuclear cells from healthy volunteers (n = 64) and HAdV-infected stem cell recipients (n = 26) for memory T cells recognizing the candidate epitopes in the context of most common HLA alleles. (biomedcentral.com)
  • Functional CD8 + T cells recognizing seven epitopes were identified, among them four penton-derived and two hexon-derived peptides. (biomedcentral.com)
  • Predicted epitopes were synthesized in the form of synthetic long peptides and tested in immune system sensitization assays involving unfractionated peripheral blood mononuclear cells (PBMC). (ndltd.org)
  • Briefly, PBMC were subjected to a two-step culture, first synchronizing their exposure to the long peptides with aggressive surrogate activation of innate immunity, followed by IL-7-modulated T-cell hyperexpansion. (ndltd.org)
  • 0.0001 and p=0.0048, for CD4+ and CD8+ Ag-specific T-cells, respectively, compared to T-cells directed against peptides from non-hot spot regions). (ndltd.org)
  • Hot spot" peptide Ag-specific T-cells preferentially recognized endogenous tumor derived MUC1, either in MUC1 expressing tumor cell killing assays (p=0.038, compared to non-peptide Ag-specific T-cells) or as MUC1 tumor lysate when pulsed onto restimulatory PBMC (p=0.022 and 0.025, for CD4+ and CD8+ T-cells, respectively, compared to T-cells directed against peptides from non-hot spot regions). (ndltd.org)
  • Here, we describe for the first time a streamlined pipeline for the generation of personalized cancer vaccines starting from the isolation and selection of the most immunogenic peptide candidates expressed on the tumor cells and ending in the generation of efficient therapeutic oncolytic cancer vaccines. (elifesciences.org)
  • Spike glycoprotein (PDB Id: 6VSB) of the coronavirus was selected and analysed using immune epitope database server for prediction of potential immunogenic B and T-cell epitopes. (ijpsonline.com)
  • We aimed at identifying further targets of protective anti-HAdV immune response and characterizing immunogenic epitopes. (biomedcentral.com)
  • The best multi-epitope of the possible immunogenic property was created by combining epitopes. (springeropen.com)
  • citation needed] MHC class I and II epitopes can be reliably predicted by computational means alone, although not all in-silico T cell epitope prediction algorithms are equivalent in their accuracy. (wikipedia.org)
  • Platforms include Epibaseâ„¢ In Silico for the prediction of T cell epitopes and Epibaseâ„¢ In Vitro for the assessment of T cell and B cell responses in human donor PBMC. (pegsummit.com)
  • Clustered Iridoviridae subfamilies possess host-specific characteristics, which can be considered as exclusive features for in-silico prediction of effective epitopes for vaccine development. (biomedcentral.com)
  • Our proposed voting mechanism based system provides a novel approach for in silico LE prediction prior to vaccine development, and it is especially powerful for analyzing antigen sequences with exclusive features between two clustered groups. (biomedcentral.com)
  • Potential allergenicity of a protein is a complex issue and various tests can be made for the prediction, includes in silico as well as in vitro digestibility and binding antisera of allergic patients. (ispub.com)
  • In silico immune evaluation using C-ImmSim server showed that the peptide could concurrently elicit cell-mediated and humoral immune responses. (ijpsonline.com)
  • This research is entirely reliant on the development of epitope-based vaccines, and these in silico findings would represent a major step forward in the development of a vaccine that might eradicate SARS-CoV-2 in Bangladeshi patients. (springeropen.com)
  • epitope immunogenicity prediction through in silico TCR-peptide contact potential profiling. (biostars.org)
  • In contrast, epitope-based in silico vaccine designing can save time, money, and energy. (springeropen.com)
  • In the current study immunoinformatics procedures were employed to introduce a novel multi-epitope subunit vaccine. (ijpsonline.com)
  • An immunoinformatics study was conducted to determine the highly conserved antigenic epitope regions of hemagglutinin (HA) and neuraminidase (NA) genes in the humoral immunity and CD4+ and CD8+ T cellular immunity between 2009 pandemic H1N1 (pH1N1) and seasonal H1N1 (sH1N1) viruses. (biomedcentral.com)
  • In this study, the highly conserved antigenic epitopes sequences and the locations of HA and NA proteins, including B-cell epitopes, MHC I and MHC II epitopes between pH1N1 and sH1N1 viruses, based on immunoinformatics, were analyzed. (biomedcentral.com)
  • However, limited data are available about sequence variability in CD8 + T cell epitope regions of mumps virus (MuV) proteins. (mdpi.com)
  • Although epitopes are usually non-self proteins, sequences derived from the host that can be recognized (as in the case of autoimmune diseases) are also epitopes. (wikipedia.org)
  • Another technique involves high-throughput mutagenesis, an epitope mapping strategy developed to improve rapid mapping of conformational epitopes on structurally complex proteins. (wikipedia.org)
  • Potential B-cell and T-cell epitopes were predicted for nine antigenic EBV proteins that mediate epithelial cell-attachment and spread, capsid self-assembly, DNA replication and processivity . (ojcius.net)
  • Results Computational predictions and sequence overlapping analysis yielded 18 linear (continuous) and discontinuous (conformational) subunit epitopes from the antigenic proteins with characteristic surface accessibility, flexibility and antigenicity, and predictive scores above the threshold value (1) set. (ojcius.net)
  • The prediction of an allergenic protein is important presently due to the specific use of modified proteins in edible foods, therapeutics and biopharmaceuticals (Goodman et al 2005). (ispub.com)
  • Different types of transgenic proteins food allergen proteins sequences were scanned for IgE epitopes. (ispub.com)
  • We have scanned total 52 proteins for detection of allergenic IgE epitopes. (ispub.com)
  • We use two evaluation datasets consisting of known HIV CTL epitopes and their source proteins. (biomedcentral.com)
  • Nineteen candidate nonamers from hexon and penton proteins were identified by epitope binding prediction. (biomedcentral.com)
  • By performing large-scale major histocompatibility complex (MHC) II analyses on HA proteins, the degree of T-cell cross-reactivity between sH1N1 from 1968 to 2009 and pH1N1 strains was investigated. (biomedcentral.com)
  • There were compared with epitopes in the Immune Epitope Database (IEDB) which contains experimentally identified epitopes in sH1N1 HA proteins, which may be helpful in design of common antigens of pH1N1 influenza and sH1N1 viruses. (biomedcentral.com)
  • This multi-epitope vaccine can activate equally class I and II human leukocyte antigen and antibody mediated immune responses. (ijpsonline.com)
  • The two dimensional and three dimensional structure of multi-epitope vaccine were predicted and authenticated using PROCheck and Raptor-X servers. (ijpsonline.com)
  • Docking results of the multi-epitope vaccine peptide with human leukocyte antigen class I and II alleles predicted efficient binding and the resulted docked models were stable during simulation. (ijpsonline.com)
  • In this study, pneumococcal surface protein A (PspA), one of the major virulence factors of SPN, is used to design a multi-epitope vaccine. (springeropen.com)
  • The constructed multi-epitope vaccine showed a strong binding affinity with the receptor molecule TLR4. (springeropen.com)
  • However, not all MHC-I binders are T cell epitopes, and multiple studies have investigated what additional factors are important for determining the immunogenicity of a peptide. (iedb.org)
  • Presentations at Immunogenicity Prediction and Mitigation will enable attendees to make informed decisions on candidate selection, dosing and formulation, with a view to producing safe and efficacious products in the clinic and beyond. (pegsummit.com)
  • The immunogenicity, antigenicity scores, and toxicity of these epitopes were evaluated and chosen based on their capacity to elicit an immune response. (springeropen.com)
  • In contrast, a linear epitope is formed by the 3-D conformation adopted by the interaction of contiguous amino acid residues. (wikipedia.org)
  • A linear epitope is not determined solely by the primary structure of the involved amino acids. (wikipedia.org)
  • Recently, the first set of naturally presented human leukocyte antigen Class I (HLA-I) epitopes of MuV was identified by us. (mdpi.com)
  • An epitope, also known as antigenic determinant, is the part of an antigen that is recognized by the immune system, specifically by antibodies, B cells, or T cells. (wikipedia.org)
  • Conformational and linear epitopes interact with the paratope based on the 3-D conformation adopted by the epitope, which is determined by the surface features of the involved epitope residues and the shape or tertiary structure of other segments of the antigen. (wikipedia.org)
  • T cell epitopes are presented on the surface of an antigen-presenting cell, where they are bound to major histocompatibility complex (MHC) molecules. (wikipedia.org)
  • The part of the antigen that immunoglobulin or antibodies bind to is called a B-cell epitope. (wikipedia.org)
  • Adoptive cell therapy using engineered T-cell receptors (TCRs) targeting cancer-testis antigens, such as Melanoma-associated antigen 3 (MAGE-A3), is a potential approach for the treatment of NSCLC. (thno.org)
  • However, systematic analysis of T cell immune responses to MAGE-A3 antigen and corresponding antigen-specific TCR is still lacking. (thno.org)
  • In this study, we comprehensively screened HLA-A2 restricted MAGE-A3 tumor epitopes and characterized the corresponding TCRs using in vitro artificial antigen presentation cells (APC) system, single-cell transcriptome and TCR V(D)J sequencing, and machine-learning. (thno.org)
  • Although a limited number of immunodominant peptide epitopes are consistently observed in diseases such as HIV-1 infection, the relationship between immunodominance and antigen processing in humans is largely unknown. (jci.org)
  • This association afforded us a unique opportunity to identify a sarcoidosis-specific antigen recognized by AV2S3+ T cells. (jci.org)
  • To identify candidates for the postulated sarcoidosis-specific antigen, lung cells from 16 HLA-DRB1*0301pos patients were obtained by bronchoalveolar lavage. (jci.org)
  • 1] Ansari, H. R., Raghava, G. P., Identification of conformational B-cell Epitopes in an antigen from its primary sequence. (degruyter.com)
  • We hypothesized that many tumor associated antigens (Ag) are readily susceptible to immune attack, but only in the context of identifying the tumor antigen epitopes that can reliably initiate an immune response, regardless of individual patient human leukocyte antigen (HLA) haplotype restrictions. (ndltd.org)
  • Utilizing known HLA-serotype frequencies and setting a threshold of ninety percent of population coverage, regardless of race or ethnicity, twenty-nine different HLA-DRB1 haplotypes were chosen for antigen prediction utilizing the open source epitope prediction algorithm netMHCIIpan. (ndltd.org)
  • Here, in the context of avian H7N9 influenza, we present a novel antigen design strategy combining immunoinformatic and structure modeling systems to harness both T cell and B cell immune mechanisms to produce more effective vaccines. (fabretp.org)
  • On the basis of predicted epitopes vaccines can be designed with better specificity. (envirobiotechjournals.com)
  • The premise for this vaccine concept rests on (i) the significance of CD4+ T cell memory space to influenza immunity, (ii) the essential role CD4+ T cells perform in development of neutralizing antibodies, (iii) linked specificity of HA-derived CD4+ T cell epitopes to antibody reactions, (iv) the structural plasticity of HA and (v) an illustration of improved antibody response to a prototype manufactured recombinant H7-HA vaccine. (fabretp.org)
  • a webtool for the prediction of T-cell receptor sequence epitope specificity. (biostars.org)
  • Methods Predictive algorithms incorporated in the Immune Epitope Database (IEDB) resources were used to determine potential B-cell epitopes based on their physicochemical attributes. (ojcius.net)
  • It serves as a companion site to the Immune Epitope Database (IEDB) , a manually curated database of experimentally characterized immune epitopes. (biostars.org)
  • I believe that IEDB predicts MHC - epitope binding. (biostars.org)
  • The results support the application of this approach for epitope selection and reveal informative features of these naturally occurring epitopes to aid in epitope prioritization for use in tumor vaccines. (broadinstitute.org)
  • Little is known of the T cell repertoire these recombinant vaccines induce. (biomedcentral.com)
  • T cells primed by these recombinant adenovirus vaccines expanded after PPRV challenge and probably contributed to protection. (biomedcentral.com)
  • Current HIV vaccines are designed to elicit both T-cell and B-cell responses. (iucc.ac.il)
  • Any cross-reactive memory space B and T cells would be present at frequencies too low to confer protecting antibody immunity by seasonal vaccination.18 As a consequence, avian influenza vaccines require higher doses than seasonal vaccines or adjuvant formulation to stimulate. (fabretp.org)
  • The results suggested that highly conserved antigenic epitope regions might act as the basis of common antigenic vaccines against pH1N1 and sH1N1 viruses. (biomedcentral.com)
  • NetCTL 1.2 algorithms enabled the prediction of probable T-cell epitopes which were structurally modeled and subjected to blind peptide-protein docking with HLA-A*02:01. (ojcius.net)
  • With advances in the accuracy of predictive algorithms for MHC class I binding, choosing epitopes on the basis of predicted affinity provides a rapid and unbiased approach to epitope prioritization. (broadinstitute.org)
  • Bioinformatics techniques are used to predict B cell and T cell epitopes from the new SARS-CoV-2 spike glycoprotein in order to build a unique multiple epitope vaccine. (springeropen.com)
  • Several CTL epitopes, HTL epitopes, and LBL epitopes of PspA were all predicted by using several bioinformatics tools. (springeropen.com)
  • These were combined with a string-kernel method and an antigenicity predictive AlgPred tool to enhance accuracy in the end-point selection of highly potential antigenic EBV B-cell epitopes. (ojcius.net)
  • In the present study, sequences of 40 CD8 + T cell epitope candidates, including previously and newly identified, obtained from Jeryl-Lynn mumps vaccine strains were compared with genomes from 462 circulating MuV strains. (mdpi.com)
  • Furthermore, specific point mutations in these portable flanking sequences modulated the production and antigenicity of epitopes. (jci.org)
  • Jagat Singh Chhauhan, Adil H. Bhat, Alka Rao and Gajendra pal Singh Raghava : GlycoPP: A Webserver for Prediction of N- O-Glycosites in Prokaryotic Protein Sequences. (imtech.res.in)
  • Say I already have both epitode and sequences of T/B cell CDR3 region available. (biostars.org)
  • As such, peptide-MHC (pMHC) binding affinity predictors are currently the premier methods for T cell epitope prediction, and these prediction methods have been shown to have high predictive performances in multiple studies. (iedb.org)
  • The integrated model leads to a consistent and significant increase in predictive performance and we demonstrate how this can be utilized to decrease the experimental workload of epitope screens. (iedb.org)
  • It was found that in sH1N1 viruses, 29 epitope regions of HA genes and 8 epitope regions of NA genes which had been experimentally identified, were highly conserved (97.1-100.0%) in the corresponding genes and predictive epitopes of the pH1N1 viruses. (biomedcentral.com)
  • If an antibody binds to an antigen's epitope, the paratope could become the epitope for another antibody that will then bind to it. (wikipedia.org)
  • Harinder Singh, Hifzur Rahman Ansari and Gajendra pal Singh Raghava: LBtope: Improved method for linear B-cell epitope prediction using antigen's primary sequence. (imtech.res.in)
  • There are two main methods of epitope mapping: either structural or functional studies. (wikipedia.org)
  • Methods for structurally mapping epitopes include X-ray crystallography, nuclear magnetic resonance, and electron microscopy. (wikipedia.org)
  • Methods for functionally mapping epitopes often use binding assays such as western blot, dot blot, and/or ELISA to determine antibody binding. (wikipedia.org)
  • All-atom molecular dynamics (MD) simulation and Molecular Mechanics Generalized-Born Surface Area methods were used to investigate interaction dynamics and affinities of predicted T-cell peptide-protein complexes. (ojcius.net)
  • At least four other methods have been developed recently that likewise attempt to predict CTL epitopes: EpiJen, MAPPP, MHC-pathway, and WAPP. (biomedcentral.com)
  • In order to compare the performance of prediction methods, objective benchmarks and standardized performance measures are needed. (biomedcentral.com)
  • In the RANK measure, we compare two methods at a time and count how often each of the methods rank the epitope highest. (biomedcentral.com)
  • Several other groups have likewise attempted to generate methods that enable CTL epitope identification. (biomedcentral.com)
  • We atory sensitisation [7] , there is now consensus that it can present a review of the available prediction methods and occur when an immune mechanism cannot be demon- propose a novel, high-throughput protocol. (cdc.gov)
  • Neotopes are epitopes that are only recognized while in a specific quaternary structure and the residues of the epitope can span multiple protein subunits. (wikipedia.org)
  • Mutagenesis uses randomly/site-directed mutations at individual residues to map epitopes. (wikipedia.org)
  • Notably, cytosolic digestion experiments revealed flanking residues proximal to the immunodominant epitope that increased the production and antigenicity of otherwise subdominant epitopes. (jci.org)
  • Bharat Panwar, Sudheer Gupta and Gajendra Pal Singh Raghava : VitaPred- Software for the sequence based prediction of vitamin interacting residues using evolutionary information. (imtech.res.in)
  • Bharat Panwar and Gajendra Pal Singh Raghava : Prediction and Classification of Nucleic Acid Interacting Residues in a Protein using Composition Profile of Patterns. (imtech.res.in)
  • Epitopes that are masked when protein subunits aggregate are called cryptotopes. (wikipedia.org)
  • Some viruses possess an outer envelope, and the outer capsid is composed of major capsid protein (MCP), which appears to be highly conserved among the family and possesses surface binding sites interacting with the surfaces of host's cells [ 2 ]. (biomedcentral.com)
  • EvoRator: prediction of residue-level evolutionary rates from protein structures using machine learning. (tau.ac.il)
  • Hence, T-and B- (IgE) epitopes are both relevant targets for models and the detection of protein allergens. (ispub.com)
  • Population conservation studies of the selected protein and predicted epitopes were also performed using population conservancy analysis tool of immune epitope database resource. (ijpsonline.com)
  • Serine protease present on cell membrane, is used by SARS-CoV-2 for associating spike protein to enable its fusion with host cells[ 12 - 14 ]. (ijpsonline.com)
  • Multiple factors determine the ability of a peptide to elicit a cytotoxic T cell lymphocyte response. (iedb.org)
  • Relative Mouse monoclonal to CDC2 to seasonal influenza, vaccination against avian influenza poses a unique challenge because the human population is definitely immunologically na?ve.17 Vaccination cannot rely on preferential recruitment of memory space B and T cells to elicit a protective antibody response due to Diethyl aminoethyl hexanoate citrate distant sequence relatedness with seasonal influenza. (fabretp.org)
  • From biological studies using peripheral blood mononuclear cell (PBMC) from human donors not previously exposed to the pandemic virus, pre-existing CD4+ T cells can elicit cross-reactive effector responses against the pandemic H1N1 virus. (biomedcentral.com)
  • X-ray crystallography of Ag-Ab complexes is considered an accurate way to structurally map epitopes. (wikipedia.org)
  • Evaluation of the ability of AlphaFold to predict the three-dimensional structures of antibodies and epitopes. (tau.ac.il)
  • In atopic individuals, sensitizing T-cell epitopes can trigger a cascade of events that leads to synthesis of allergen-specific immunoglobulin E (IgE) antibodies as well as other immunological reactions. (ispub.com)
  • Based on the above observations, it was postulated that the plasmablasts that produced these broadly neutralizing antibodies were predominantly derived from activated memory B cells specific for epitopes conserved in some influenza strains. (biomedcentral.com)
  • Conclusion This study implemented an integrative meta-analytical approach to model highly probable B-cell and T-cell epitopes as potential peptide-vaccine candidates for the treatment of EBV-related diseases. (ojcius.net)
  • Cross-reactivity risk analysis of the candidates TCRs showed high binding stability to MAGE-A3-Mp4 epitope and low risk of cross-reaction. (thno.org)
  • We further hypothesized that epitope prediction strategies which seek to identify pan- or highly promiscuous-HLA binding epitopes would reduce the number of potential candidates and be more likely to accurately identify high-priority tumor Ag epitopes. (ndltd.org)
  • Here, we have demonstrated that endogenous processing and presentation of a human immunodominant HIV-1 epitope is more efficient than that of a subdominant epitope. (jci.org)
  • Furthermore, we have shown that the regions flanking the immunodominant epitope constitute a portable motif that increases the production and antigenicity of otherwise subdominant epitopes. (jci.org)
  • We used a novel in vitro degradation assay involving cytosolic extracts as well as endogenous intracellular processing assays to examine 2 well-characterized HIV-1 Gag overlapping epitopes presented by the same HLA class I allele, one of which is consistently immunodominant and the other subdominant in infected persons. (jci.org)
  • Identification of new immunodominant epitopes will facilitate and broaden immune assessment strategies to identify patients suitable for T-cell transfer. (biomedcentral.com)
  • Electron microscopy is a low-resolution method that can localize epitopes on larger antigens like virus particles. (wikipedia.org)
  • The latter is a tool previously developed by Jacopo, 2020, able to identify tumor antigens similar to pathogen antigens in order to exploit molecular mimicry and tumor pathogen cross-reactive T cells in cancer vaccine development. (elifesciences.org)
  • We show herein the retrospective application of a prediction algorithm to a large set of bona fide T cell-defined mutated human tumor antigens that induced immune responses, most of which were associated with tumor regression or long-term disease stability. (broadinstitute.org)
  • sequence-based prediction of TCR binding to peptide-MHC complexes using convolutional neural networks. (biostars.org)
  • Risk assessment includes deciding if any of the available prediction tools should be utilized. (pegsummit.com)
  • Finally, we add, as a disclaimer, that this review by design is focused on the work produced by our laboratory as an illustration of approaches to the study of T cell responses to Mtb and BP, and is not meant to be comprehensive, nor to detract from the excellent work performed by many other groups. (ytiancompbio.com)
  • Although immune checkpoint blockade (ICB) reinvigorates anti-tumor immune responses by disrupting co-inhibitory T-cell signaling, relapse frequently occurs after ICB treatment and acquired resistance often emergence after initial response [ 4 ]. (thno.org)
  • Such portable epitope processing determinants provide what we believe is a novel approach to optimizing CTL responses elicited by vaccine vectors. (jci.org)
  • Efficient antiviral T-cell responses are necessary to clear infection, which is hampered by delayed immune reconstitution and medical immunosuppression after HSCT. (biomedcentral.com)
  • A SARS-CoV-2 T cell epitope homologous to a validated human Treg epitope (Tregitope) suppresses CD4 and CD8 T cell memory responses Shah Md. Shahjahan Miah, Andres Gutierrez, Sandra Lelias, Christine M. Boyle, Lenny Moise, Anne S. De Groot EpiVax Inc, Providence, RI. (epivax.com)
  • We also show that recombinant adenovirus vaccination induced T cell responses to the same epitopes, and led to memory T cell differentiation. (biomedcentral.com)
  • A common endpoint in any T-cell based vaccine trial are measurements of vaccine-induced T-cell responses such as breadth and magnitude [7]. (iucc.ac.il)
  • Given information about participant's HLA alleles, we attempt to predict vaccine induced T-cell responses at various levels of refinement, based on the current group-testing hierarchical mapping approach. (iucc.ac.il)
  • Accordingly, the role of TLR4 was investigated towards CHIKV infection and modulation of host immune responses in the current study using mice macrophage cell line RAW264.7, primary macrophage cells of different origins and in vivo mice model. (bvsalud.org)
  • After checking the antigenicity, allergenicity, and toxicity scores, the best epitopes were selected for the vaccine construction, and then, physicochemical and immunological properties were analyzed. (springeropen.com)
  • The globally harmonized in the workplace are known as respiratory sensitizers, but system (GHS) for classification and labelling of chemicals this term is not used for workplace substances that aggra- ranks respiratory sensitization with other toxic effects of vate preexisting asthma or cause acute irritant-induced highest concern: carcinogenicity, germ cell mutagenicity asthma, also known as the reactive airways dysfunction and reproductive toxicity [4] . (cdc.gov)
  • The approaches used in this research are ABCpred, BCEpred and BEPIpred for epitope prediction in swine strain of hepatitis E virus which is the causative agent of hepatitis E, is a non-enveloped RNA virus. (envirobiotechjournals.com)
  • Neoantigen-reactive T cell clonotypes increased and persisted in peripheral blood after treatment. (nature.com)
  • In this study, we investigate if improved accuracy for T cell epitope discovery can be achieved by integrating predictions for pMHC binding affinity, pMHC stability, and T cell propensity. (iedb.org)
  • We show that a weighted sum approach allows pMHC stability and T cell propensity predictions to enrich pMHC binding affinity predictions. (iedb.org)
  • A novel site was identified on HLA-A*02:01 with preferential affinity binding for modeled BMRF2, BXLF1 and BGLF4 T-cell epitopes. (ojcius.net)
  • It does so by integrating predictions of proteasomal cleavage, TAP transport efficiency, and MHC class I affinity. (biomedcentral.com)
  • Contagious human coronavirus (SARS-CoV-2) binds to the receptors of human host cells by transmembrane spike glycoprotein that makes homotrimers protruding from viral surface region[ 9 , 10 ]. (ijpsonline.com)
  • Spike glycoprotein has two subunits, S1-responsible for its binding to host cell membrane receptors and S2-responsible for the fusion of viral and host cellular membrane[ 8 , 11 ]. (ijpsonline.com)
  • Epitopes are relational entities that requires complementary paratopes for their operational recognition. (envirobiotechjournals.com)
  • Importantly, T cells artificially expressing the MAGE-A3-Mp4 specific TCRs exhibited strong MAGE-A3+ tumor cell recognition and killing effect. (thno.org)
  • Peptide presentation is critical for immune recognition of pathogen-infected cells by CD8+ T lymphocytes. (jci.org)
  • As immunity against influenza viruses involved not only B cell activation, but also T cell recognition and presentation, the T cell subgroups undoubtedly play an important role. (biomedcentral.com)
  • Predictions resulted in identification of highly promiscuous-HLA binding epitopes. (ndltd.org)
  • First, we are interested in Epstein Barr Virus-mediated mechanisms of immune evasion with particular emphasis on pathways that promote survival and proliferation of EBV B cell lymphomas, the characterization of the human T cell and NK cell response to EBV and the identification of novel therapeutics for treatment of EBV B cell lymphomas. (stanford.edu)
  • Predictions were also performed for HLA-A serotypes utilizing the open source algorithm netMHCpan. (ndltd.org)
  • Therefore, computationally predicted IgE epitopes from GMF allergen might be an ideal peptide-based novel candidate for immunotherapy. (ispub.com)
  • CD8 + T cells play a critical role in immunity to viruses. (mdpi.com)
  • Protective immunity may be conferred by adoptive transfer of HAdV-specific T cells. (biomedcentral.com)
  • Monitoring HAdV-specific T-cell immunity may improve risk assessment in HSCT recipients and enhance treatment efficacy by determining the optimal time point for adoptive T-cell transfer. (biomedcentral.com)
  • The part of an antibody that binds to the epitope is called a paratope. (wikipedia.org)
  • Immunology 2008, 8:103-109 relationship models for prediction of low-molecular-weight organic chemical respiratory sensitizers. (cdc.gov)
  • Fig. 5: Change in phenotype and genotype of peripheral T cells after TIL infusion. (nature.com)
  • We identified the HLA-A2 restricted T cell epitopes from MAGE-A3 that could effectively induce the activation and cytotoxicity of CD8+ T cells using artificial APC in vitro . (thno.org)
  • Moreover, this led to reduced expression of macrophage activation markers like CD14, CD86, MHC-II and pro-inflammatory cytokines (TNF, IL-6, MCP-1) significantly in both the mouse primary macrophages and RAW264.7 cell line, in vitro. (bvsalud.org)
  • Additionally, TAK-242-directed TLR4 inhibition demonstrated a significant reduction of percent E2-positive cells, viral titre and TNF expression in hPBMC-derived macrophages, in vitro. (bvsalud.org)
  • The experimental results showed that four conserved epitopes among the Iridovirideae family, one exclusive epitope for invertebrate subfamily and two exclusive epitopes for vertebrate family were predicted. (biomedcentral.com)
  • Most importantly, they can minimize the experimental effort needed to identify epitopes. (biomedcentral.com)
  • Tumor-specific neoantigens, which are expressed on tumor cells, can induce an effective antitumor cytotoxic T-cell response and mediate tumor regression. (wjgnet.com)
  • Adoptive cell therapy using tumor-infiltrating lymphocytes (TILs) has shown activity in melanoma, but has not been previously evaluated in metastatic non-small cell lung cancer. (nature.com)
  • We conducted a single-arm open-label phase 1 trial ( NCT03215810 ) of TILs administered with nivolumab in 20 patients with advanced non-small cell lung cancer following initial progression on nivolumab monotherapy. (nature.com)
  • In exploratory analyses, we found T cells recognizing multiple types of cancer mutations were detected after TIL treatment and were enriched in responding patients. (nature.com)
  • Cell therapy with autologous TILs is generally safe and clinically active and may constitute a new treatment strategy in metastatic lung cancer. (nature.com)
  • Addeo, A., Banna, G. L., Metro, G. & Di Maio, M. Chemotherapy in combination with immune checkpoint inhibitors for the first-line treatment of patients with advanced non-small cell lung cancer: a systematic review and literature-based meta-analysis. (nature.com)
  • In addition, it generates cancer stem cells (CSCs) that have the ability, following dissemination, to serve as founders of new metastatic colonies. (pegsummit.com)
  • Advanced non-small cell lung cancer (NSCLC) is the most common type of lung cancer with poor prognosis. (thno.org)
  • Non-small cell lung cancer (NSCLC), the most common subtype, comprised approximately 85% of the newly diagnosed lung cancer cases [ 2 ]. (thno.org)
  • We've screened more than 1,275 cancer cell lines as part of the Cancer Dependency Map (DepMap). (broadinstitute.org)
  • Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)
  • In the present review, we summarize work from our as well as other groups related to the characterization of bacterial T cell epitopes, with a specific focus on two important pathogens, namely, Mycobacterium tuberculosis (Mtb), the bacterium that causes tuberculosis (TB), and Bordetella pertussis (BP), the bacterium that causes whooping cough. (ytiancompbio.com)
  • Statistical and machine-learning based analyses demonstrated that the MAGE-A3-Mp4 epitope-specific CD8+ T cell clones were mostly in effector and proliferating state. (thno.org)
  • Karambir Kaur, Amit Kumar Gupta and Manoj Kumar : geCRISPR - Software for the prediction of sgRNAs genome editing efficiency for CRISPR/Cas system. (imtech.res.in)
  • In this paper we explore the idea of using HLA binding predictors to improve the efficiency of epitope mapping protocols in vaccine trials. (iucc.ac.il)
  • Using extensive epitope mapping data from a cohort of 12 acutely infected HIV infected individuals, we show that using state-of-the-art HLA binding predictors, significant improvements in mapping efficiency can be obtained. (iucc.ac.il)