• In this study, we investigated the maintenance of hESCs and their differentiation into RPE using Synthemax II-SC, which is a novel, synthetic animal-derived component-free, RGD peptide-containing copolymer compliant with good manufacturing practices designed for xeno-free stem cell culture. (ca.gov)
  • Fibroblast growth factors, in concert with other growth factors, are key regulators of lens fiber cell differentiation. (nih.gov)
  • Here we show the differentiation of patient specific iPSCs derived from a patient with SFTPB deficiency into lung organoids with mesenchymal and epithelial cell populations from both the proximal and distal portions of the human lung. (nature.com)
  • After differentiating the mutant and corrected cells into lung organoids, we show expression of SFTPB mRNA during endodermal and organoid differentiation but the protein product only after organoid differentiation. (nature.com)
  • Although many differentiation protocols in the literature have been successful in mimicking lung development from stem cells, there has not been an examination of how a specific mutation impacts the differentiation process including its effects on the early endoderm, as well as the proximal and distal lung epithelial cell populations in the lung organoids. (nature.com)
  • The adult alae are commonly used as an indicator of hypodermal seam cell terminal differentiation. (wormatlas.org)
  • Promotes embryonic stem cell (ESC) differentiation into cardiomyocytes. (fishersci.com)
  • Toward this goal, we propose to compare the behavior of nerve cell differentiation in multiple lines of hESCs in one laboratory setting. (ca.gov)
  • We will further characterize molecular changes during directed cell differentiation and identify the cells that exhibit a pattern of DNA modification, namely DNA methylation, similar to primary neural cells in human brain. (ca.gov)
  • We are now ready to compare the property of different lines of nerve cells such as the efficiency of nerve cell differentiation and the preferential production of specific nerve cells in culture. (ca.gov)
  • By engineering stem cells carrying different levels of methylation, we aim to find the optimal levels of DNA methylation for efficient nerve cell differentiation. (ca.gov)
  • Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. (nih.gov)
  • Furthermore, the decreased expression of the EZH2 gene is crucial for stem cell differentiation into specific cell lineages involved in myogenesis, adipogenesis, osteogenesis, neurogenesis, and haematopoiesis [ 9 ]. (hindawi.com)
  • At this juncture, through the present study it was found that, chicken neural retinal cells when grown alone failed to survive and contrarily when either co-cultured with chicken amniotic epithelial cells / cultured in amniotic epithelial cell conditioned medium not only survived but also showed extensive differentiation. (scielo.org.ar)
  • In recent years, epithelial-mesenchymal transition (EMT) has been suggested to play a key role in the process of embryonic development, differentiation of tissues and organs, chronic inflammation and fibrosis, as well as cancer progression ( 14 ). (spandidos-publications.com)
  • The developmental strategies used by progenitor cells to allow a safe journey from their induction place towards the site of terminal differentiation are still poorly understood. (elifesciences.org)
  • The remaining delaminated cells follow the movement of apically attached progenitors by a protrusion-dependent cell-cell contact mechanism, avoiding sequestration by the adjacent endoderm, ensuring their collective fate and allocation at the site of differentiation. (elifesciences.org)
  • These early progenitor cells often travel long distances from their induction site to the site of terminal differentiation, making them vulnerable to environmental cues and movement of neighbouring tissues. (elifesciences.org)
  • Despite the importance of the developmental pathways followed by these small groups of progenitor cells and their impact on the physiology of the organism, we still know little about the set of developmental strategies that progenitor cells deploy in vivo to overcome the challenges imposed by the environment as they travel to the site of terminal differentiation. (elifesciences.org)
  • We have used this product for in vitro smooth muscle cell differentiation from human IPSCs for more than 4 years, which contributes to several high quality publication such as Circulation, ATVB, and Nature Comm. (peprotech.com)
  • Stem cells are defined by their capacity for self-renewal and multilineage differentiation, making them uniquely situated to treat a broad spectrum of human diseases. (articlecity.com)
  • The WT1 protein mediates the mesenchymal-epithelial transition and differentiation during morphogenesis of the kidney and gonad by repressing genes that encode cell proliferation factors and by activating genes that encode markers of epithelial cell differentiation. (medscape.com)
  • Recent advances in live imaging and genetics of mammalian division, movement and cell differentiation leading to development which integrate observations of biochemical tissue formation [14 ]. (lu.se)
  • It is believed that TGF-β1 signalling is involved in controlling epithelial cell proliferation, to drive epithelial-mesenchymal transition (EMT) and it plays a major role in lung fibrosis and emphysema. (springer.com)
  • Targeted neural ablation, but not inhibition of acetylcholine receptors, halts lung branching and causes a reduction in endothelial cells and epithelial and mesenchymal proliferation. (caltech.edu)
  • Taken together, our results suggested that decreased LAPTM5 inhibited proliferation and viability, as well as induced G0/G1 cell cycle arrest possibly via deactivation of ERK1/2 and p38 in BCa cells. (spandidos-publications.com)
  • Furthermore, previous studies suggested that knockdown of LAPTM4B , another important subtype of the LAPTM family inhibited proliferation of hepatocellular carcinoma ( 11 ), prostate ( 12 ) and breast cancer cells ( 13 ). (spandidos-publications.com)
  • Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells.The expected ED 50 for this effect is 1.0-3.0 ng/ml. (peprotech.com)
  • Mitofusin-2 Restrains Hepatic Stellate Cells' Proliferation via PI3K/Akt Signaling Pathway and Inhibits Liver Fibrosis in Rats. (peprotech.com)
  • Both trials will test the safety and tolerability of retinal pigment epithelial, or RPE cells, which ACT makes from human embryonic stem cells. (foxnews.com)
  • NEW ORLEANS - Retinal pigment epithelial (RPE) cells derived from human embryonic stem cells can be safely transplanted into the eyes of patients with retinal degeneration, with early signs of vision gain, according to pioneers in the field. (medscape.com)
  • There have been several studies conducted on the potential benefits of using amniotic epithelial cells in various parts of the body. (wikipedia.org)
  • Transplantation of amniotic epithelial cells in various regions of central nervous system such as caudate nucleus (Bankiewicz et al. (scielo.org.ar)
  • What cellular mechanisms govern early morphogenesis of epithelial appendages? (helsinki.fi)
  • We have delineated a two-step AT1 cell morphogenesis process, cell flattening and cell folding, which leads to its ultrathin and yet expansive morphology. (mdanderson.org)
  • Dynamic imaging of Drosophila embryonic tracheal morphogenesis demonstrates that SERCA's principal function is to govern cell migration. (caltech.edu)
  • During embryo development, naïve cell lineages undergo concurrent processes of fate specification and morphogenesis as critical steps towards the generation of differentiated tissues and organs. (elifesciences.org)
  • Here, we examine this question during the early stages of morphogenesis of the embryonic laterality organ, the first organ to form during vertebrate development, using the zebrafish as a model organism. (elifesciences.org)
  • However, several billion cells are required to use for transplantation in order to treat and fight diseases. (wikipedia.org)
  • Therefore, the cells must be expanded in a lab to have enough cells for transplantation. (wikipedia.org)
  • Zhao Y.(2011).Mouse embryonic stem cell-derived thymic epithelial cell progenitors enhance T-cell reconstitution after allogeneic bone marrow transplantation. (ioz.ac.cn)
  • Summary of Research Progress: Our research aims to identify the optimal culture conditions and the best hESC lines for the derivation of nerve lineage cells in therapeutic cell transplantation. (ca.gov)
  • Finally, cell transplantation in a mouse stroke model will be used to study the mechanisms and efficacy of different types of hESC-derived neural cells in neural repair. (ca.gov)
  • Finally, we also made excellent progress on the procedure of cell transplantation. (ca.gov)
  • We have found a suitable substrate that can be used to enhance neuronal survival after cell transplantation and we expect to publish a research paper in this new method of cell transplantation. (ca.gov)
  • By virtue of expressions of glial and neural surface markers and capability of neurotransmitter metabolism, amniotic epithelial cells are considered as candidate cell type for transplantation strategies to treat neurological disorders. (scielo.org.ar)
  • These findings suggested the usefulness of them as an alternate source of cells for transplantation approaches to treat neurological disorders (Sakuragawa et al. (scielo.org.ar)
  • Amniotic epithelial cells transplantation in Parkinson model of rats was found to reverse the condition and prevent death in neurons (Kakishita et al. (scielo.org.ar)
  • 2001). In our previous report, we concluded the usefulness of amniotic epithelial cells transplantation in spinal cord injury repair research. (scielo.org.ar)
  • We had also outlined various biological and social advantages by which, amniotic epithelial cells transplantation excels its precedent viz. (scielo.org.ar)
  • Recently, our conclusion of usefulness of amniotic epithelial cells transplantation to treat spinal cord injuries has been confirmed in rodent models of spinal cord injury (Zhi-yuan et al. (scielo.org.ar)
  • At a median follow-up of 4 years, the researchers have seen no safety signals related to transplantation of RPE cells. (medscape.com)
  • For example, because hematopoietic stem cells can reconstitute the entire blood system, bone marrow transplantation has long been used in the clinic to treat various diseases. (articlecity.com)
  • Similarly, the transplantation of other tissue-specific stem cells, such as stem cells isolated from epithelial and neural tissues, can treat mouse disease models and human patients in which epithelial and neural cells are damaged. (articlecity.com)
  • Recent news of an impending clinical cell transplantation trial in Parkinson's disease using parthenogenetic stem cells as a source of donor tissue have raised hopes in the patient community and sparked discussion in the research community. (lu.se)
  • Based on discussions held by a global collaborative initiative on translation of stem cell therapy in Parkinson's disease, we have identified a set of key questions that we believe should be addressed ahead of every clinical stem cell-based transplantation trial in this disorder. (lu.se)
  • Significantly, there are additional non-epithelial stromal cells (NES) that exist alongside TEC within thymic microenvironments, including multiple subsets of mesenchymal and endothelial cells. (frontiersin.org)
  • Broadly separated into mesenchymal and endothelial cells, NES have been implicated in thymus organogenesis, thymocyte development, tolerance induction and development/maintenance of epithelial stroma. (frontiersin.org)
  • We are studying how lung endothelial cells respond to Vegfa during intussusceptive angiogenesis and whether and how the same Vegfa signaling elicits distinct responses from those during the well-characterized retinal sprouting angiogenesis. (mdanderson.org)
  • Induces hyperpolarization to the same magnitude as ACh in aortic value endothelial cells. (fishersci.com)
  • How are cell fates determined in the embryonic surface epithelium? (helsinki.fi)
  • In early experiments, physical separation of mesenchymal and epithelial stroma from embryonic murine thymus resulted in defective thymus development when epithelium was cultured in vitro in the absence of mesenchyme ( 7 ). (frontiersin.org)
  • Tissues obtained from amniotic cell lines show promise for patients with congenital diseases or malformations of the heart, liver, lungs, kidneys, and cerebral tissue. (wikipedia.org)
  • This makes the possibility of rejection of the cells by the recipient tissue less likely. (wikipedia.org)
  • Unlike traditional tissue transplants, where the donor and recipient have to be in complete sync as far as blood type and overall compatibility and even still can show tissue rejection, amniotic epithelial cells can be used and show a higher likelihood of tissue acceptance. (wikipedia.org)
  • Unlike embryonic stem cells, amniotic stem cells have not shown a propensity for developing into teratomas and other cancer-like tumors upon injection into living tissue. (wikipedia.org)
  • N-cadherin-mediated adhesion is essential for maintaining the tissue architecture and stem cell niche in the developing neocortex. (researchgate.net)
  • Osteoderms are composed of bone tissue , and are derived from a scleroblast neural crest cell population during embryonic development of the organism. (wikipedia.org)
  • Stem cells are found in human body in an early stage of life as well as in adults and can differentiate into specialized cells types of a tissue or an organ. (precedenceresearch.com)
  • To date, a lot of EZH2 variants have been found in various cell and tissue types [ 18 - 20 ]. (hindawi.com)
  • a ) Schematic representation of an epithelial tissue layer. (elifesciences.org)
  • The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. (peprotech.com)
  • With this raw material, biomedical firms can create stem cell lines that, among other things, aid recuperation via the regeneration of tissue that has been lost or damaged. (articlecity.com)
  • Current research is focused on growing a wide range of new tissue from stem cells, including muscle, blood, brain, and cartilage cells. (articlecity.com)
  • Adult stem cells, which are present in small amounts in adult tissue but less adaptable than embryonic stem cells, making their use in medical treatments more challenging. (articlecity.com)
  • They have many of the positive characteristics of embryonic stem cells while sourcing material is far less challenging, since postpartum tissue can be used. (articlecity.com)
  • BACKGROUND: Lipopolysaccharide (LPS) of Helicobacter pylori (Hp) bacteria causes disintegration of gastric tissue cells in vitro. (bvsalud.org)
  • At histologic examination, the cysts typically are composed of vascularized stroma of connective tissue and 3 types of epithelial cells: ciliated, nonciliated epithelial, and mucous secreting. (medscape.com)
  • of the most critical events which lead to tissue patterning involves mechanisms going beyond single cells. (lu.se)
  • Increasing attention is now being directed at the network of signaling pathways that effect the EMT in lens epithelial cells, with the aim of identifying potential therapeutic targets to inhibit cataract, particularly PCO, which remains a significant clinical problem in ophthalmology. (nih.gov)
  • Highly expressed in the mammalian lens, the α-crystallins have been postulated to play a critical role in the maintenance of lens optical properties by sequestering age-damaged proteins prone to aggregation as well as through a multitude of roles in lens epithelial cells. (nih.gov)
  • The Epithelial to Mesenchymal Transition (EMT) regulates cell plasticity during embryonic development and in disease. (researchgate.net)
  • BMP-induced epithelial to mesenchymal transition (EMT) in the lateral plate mesoderm (LPM) triggers L/R asymmetric cell movements toward the midline, higher from the right, which are crucial for heart laterality in. (researchgate.net)
  • The epithelial-mesenchymal transition (EMT), a prerequisite for cancer progression and metastasis formation, is regulated not only at the transcriptional but also at the post-transcriptional level, including at the level of alternative pre-mRNA splicing. (researchgate.net)
  • [4] Neural crest cells, through epithelial-to-mesenchymal transition, are thought to contribute to osteoderm development . (wikipedia.org)
  • d ) Planar cell neighbour exchange (T1 transition). (elifesciences.org)
  • Furthermore, proteins involved in epithelial-mesenchymal transition (EMT) were strongly altered, which plays a central role in metastasis. (spandidos-publications.com)
  • Extensive research on thymic epithelial cells (TEC) within the cortex and medulla has defined their essential roles during T-cell development. (frontiersin.org)
  • Arguably, the most recognized and well-studied stromal populations in thymus biology are thymic epithelial cells (TEC) within the cortical and medullary areas, which are defined by their anatomical separation and function. (frontiersin.org)
  • Amniotic epithelial cells have not been known to produce cancerous tumors in the host despite the fact that these cells do express genes found in embryonic stem cells that are known to promote tumor formation. (wikipedia.org)
  • It is now clear that Armadillo and beta-catenin bind directly to members of the T-cell factor/lymphoid enhancer factor subfamily of HMG box DNA-binding proteins, forming bipartite transcription factors that regulate Wingless/Wnt responsive genes in both Drosophila and vertebrates. (embl.de)
  • Found within Wharton's jelly - which is easily harvested from what would otherwise be post-natal medical waste - are several distinct stem cell genes. (articlecity.com)
  • We conclude that the observed effects contribute to loss of epithelial integrity during atopic asthma. (springer.com)
  • Artificial heart valves and working tracheas, as well as muscle, fat, bone, heart, neural and liver cells have all been engineered using amniotic stem cells. (wikipedia.org)
  • The scleroblastic neural crest cell population shares some homologous characteristics associated with the dermis . (wikipedia.org)
  • Further studies will establish the nature of this novel factor which will enhance the application of this interesting cell type for neural transplantations. (scielo.org.ar)
  • CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
  • Lung epithelial cells form a barrier that lines airway and alveolar surfaces and constitute an air-liquid interface. (springer.com)
  • Herein we elaborated effects of TGF-β1 on TJs of primary human bronchial epithelial cells. (springer.com)
  • The spatial sequence of branching of bronchial epithelial tubes is stereotyped. (caltech.edu)
  • We strive to understand the molecular and cellular regulation of epithelial appendage (hair, mammary gland, tooth) development and how they are perturbed in diseases such as congenital disorders or tumorigenesis. (helsinki.fi)
  • Lung development is a complicated process that involves the specialization of multiple bronchiolar and alveolar epithelial populations. (nature.com)
  • The thymus supports T-cell development via specialized microenvironments that ensure a diverse, functional and self-tolerant T-cell population. (frontiersin.org)
  • However, experimental evidence suggests these cells are important for thymus function by either directly influencing T-cell development, or by indirectly regulating TEC development and/or function. (frontiersin.org)
  • While adult lymphoid progenitor cells are produced by the bone marrow, their lineage commitment and development into mature T-cells is dependent on their migration into the thymus, where essential interactions with heterogeneous thymic stromal cells take place ( 1 ). (frontiersin.org)
  • While many studies have demonstrated the importance of cTEC and mTEC during T-cell development, thymic stromal microenvironments also contain heterogenous non-epithelial stromal (NES) populations in a similar manner to that seen in peripheral lymphoid tissues. (frontiersin.org)
  • Thus, our findings have implicated AT1 cell development and its regulator in the pathogenesis of BPD. (mdanderson.org)
  • Transcriptional regulation of epithelial and endothelial cell functions during lung embryonic development and lung carcinogenesis. (cincinnatichildrens.org)
  • Our research aims to elucidate the molecular basis of lung development and disease with specific focus on identifying critical biologic functions of the Rb/p16, p53 and Dicer/miRNA pathways in pulmonary progenitor/stem cell growth in the context of organogenesis, repair after injury and disease pathogenesis to identify novel targets with diagnostic and therapeutic utility. (cincinnatichildrens.org)
  • Due to constant research and development activities in the stem cell treatment market there has been a growth in the demand for the gene treatments and the cell treatments with the help of the stem cells. (precedenceresearch.com)
  • EZH2, a methyltransferase catalyzing H3K27me3, has been abundantly studied in human and mouse embryonic development. (hindawi.com)
  • These results validated a different transcript in pigs and characterized its expression profile in fetal tissues of different gestation stages, which indicated that EZH2 played important roles during porcine embryonic development. (hindawi.com)
  • In the early embryonic development, abnormal expression of EZH2 impaired embryo growth and pluripotency maintenance [ 7 , 8 ]. (hindawi.com)
  • Thus, the appropriate expression level of the EZH2 gene is important for embryonic development. (hindawi.com)
  • In pigs, several studies have focused on its function in early embryonic development regulation and in SCNT efficiency improvement [ 8 , 14 - 16 ]. (hindawi.com)
  • However, the transcript and expression status of the EZH2 gene during porcine embryonic development remains unknown. (hindawi.com)
  • Subsequently, we detected the expression of EZH2 on mRNA level and protein level in two different embryonic development stages (65-dpc and 90-dpc) via qRT-PCR and western blots. (hindawi.com)
  • Our study is aimed at providing information for understanding the biological function of EZH2 in porcine embryonic development. (hindawi.com)
  • The FREM1 protein is one of a group of proteins, including proteins called FRAS1 and FREM2, that interact during embryonic development as components of basement membranes. (medlineplus.gov)
  • Absence of functional FREM1 protein interferes with its role in embryonic basement membrane development and may also affect the location, stability, or function of the FRAS1 and FREM2 proteins. (medlineplus.gov)
  • Embryonic development occurs with precisely timed morphogenetic cell movements directed by complex gene regulation. (duke.edu)
  • Lung development is a complex process orchestrated by as many as 40 different types of cells and many signaling and regulatory factors. (caltech.edu)
  • Similarly, during Drosophila embryonic development, SERCA activity is required for the proper formation of both the central nervous system axon tracts and the network of tracheal tubules that deliver oxygen to tissues. (caltech.edu)
  • Together, these finding reveal that SERCA regulates cell migration, and this serves as a conserved mechanism that governs branch formation in various cell types and species during development. (caltech.edu)
  • These abnormalities were less frequent in transgenic zebrafish embryos expressing rat αA-crystallin suggesting a specific role of α-crystallins in embryonic lens development. (nih.gov)
  • These findings demonstrate that the role of α-crystallins in lens development is conserved from mammals to zebrafish and set the stage for using the embryonic lens as a model system to test mechanistic aspects of α-crystallin chaperone activity and to develop strategies to fine-tune protein-protein interactions in aging and cataracts. (nih.gov)
  • These partners not only play key roles in a variety of cell fate decisions during normal development but, when inappropriately activated, contribute to both colon cancer and melanoma. (embl.de)
  • The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. (peprotech.com)
  • Embryonic stem cells, which must be extracted from embryos three to five days old (known as blastocysts, which contain only about 150 cells at this point of development). (articlecity.com)
  • Induced pluripotent stem cells, which harness the power of genetic reprogramming - basically, the altering of a cell's DNA - to change the course of cellular development. (articlecity.com)
  • Development: For advances in developmental biology and stem cells. (lu.se)
  • An embryonic mouse lung showing the epithelial progenitors in green and the airways in magenta. (mdanderson.org)
  • Here, we uncovered a mechanism of progenitor cell allocation that stems from an incomplete process of epithelial delamination that allows progenitors to coordinate their movement with adjacent extra-embryonic tissues. (elifesciences.org)
  • Progenitors of the zebrafish laterality organ originate from the superficial epithelial enveloping layer by an apical constriction process of cell delamination. (elifesciences.org)
  • During this process, progenitors retain long-lasting apical contacts that enable the epithelial layer to pull a subset of progenitors on their way to the vegetal pole. (elifesciences.org)
  • In this pyloric metaplasia, corpus glands become antrum (pylorus)-like with loss of acid-secreting parietal cells (atrophic gastritis), expansion of foveolar cells, and reprogramming of digestive enzyme-secreting chief cells into deep antral gland-like mucous cells. (bvsalud.org)
  • A bone biopsy specimen was taken in the Department of Oral and Maxillofacial Surgery and the histopathology revealed pseudo-cystic and ductal mucin pooled spaces lined by mucous cells and squamous or epidermoid cells. (bvsalud.org)
  • Human alveolar adenocarcinoma epithelial A549 cells were maintained in Ham's F12-K containing 10% FCS. (cdc.gov)
  • Surfactant is produced by alveolar type II cells which can be differentiated in vitro from patient specific induced pluripotent stem cell (iPSC)-derived lung organoids. (nature.com)
  • Alveolar type II (ATII) epithelial cells synthesize, secrete and recycle all components of surfactant and dysfunction in surfactant metabolism can result in a variety of pediatric lung diseases including respiratory distress syndrome and interstitial lung disease 3 . (nature.com)
  • Current BPD research focuses on myofibroblasts, alveolar type 2 (AT2) cells and the endothelium, but seems to leave out the "elephant in the room", the alveolar type 1 (AT1) cell, which constitutes nearly all the alveolar surface and associates intimately with the microvasculature. (mdanderson.org)
  • We have identified a key transcriptional regulator of this process, without which AT1 cells lose their molecular and cellular characteristics and the lung undergoes alveolar simplification as in BPD. (mdanderson.org)
  • We have found that AT1 cells, instead of AT2 cells, are the unexpected source of a key angiogenic factor VEGFA and AT1 cell-derived VEGFA is required for alveolar angiogenesis. (mdanderson.org)
  • The generation of different cell types and specialized organs. (cincinnatichildrens.org)
  • Several embryonic tissues and organs originate from small sets of progenitor cells. (elifesciences.org)
  • Лютеїнізуючий гормон (ЛГ) та фолікулостимулюючий гормон (ФСГ) The endocrine system coordinates functioning between different organs through hormones, which are chemicals released into the bloodstream from specific types of cells within endocrine (ductless). (msdmanuals.com)
  • Cortical epithelial cells (cTEC) support the earliest thymocyte progenitor populations, guiding them through the cortex, directing them to a T-cell lineage fate and ensuring their functional qualities as self-MHC restricted cells through the process of positive selection ( 2 ). (frontiersin.org)
  • Molecular mechanisms of childhood liver cancer with a focus on hepatic stem/progenitor cells. (cincinnatichildrens.org)
  • 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
  • Cell cycle analysis suggested activation of apoptosis, without arresting cell cycle progression. (nature.com)
  • Annexin V-propidium iodide double-staining demonstrated that Sapota fruit extracts potentiate apoptosis rather than necrosis in cancer cells. (nature.com)
  • Finally, analysis of the mechanism by which multimeric alpha-lactalbumin induces apoptosis in transformed epithelial cells could lead to the design of antitumor agents. (lu.se)
  • In contrast, no significant alteration of apoptosis in the BCa cells with downregulated LAPTM5 was noticed. (spandidos-publications.com)
  • Then cell migration was assessed in conjunction with oxidative stress and apoptosis, activation of extracellular signal-regulated kinase (Erk), production of collagen I and soluble ST2 (IL-33 decoy). (bvsalud.org)
  • Multimeric alpha-lactalbumin killed all transformed, embryonic, and lymphoid cells tested but spared mature epithelial elements. (lu.se)
  • After acute parietal cell loss, chief cells can reprogram through an orderly stepwise progression (paligenosis) initiated by interleukin-13-secreting innate lymphoid cells (ILC2s). (bvsalud.org)
  • Between the L2 and L4 stages, most seam-cell divisions generate an anterior daughter cell that fuses with hyp 7 and a posterior daughter cell that continues to divide until the last molt between L4 and adult stages (see HypFIG 1 ). (wormatlas.org)
  • During embryonic stages of lung organogenesis, TGF-β1 is pivotal in epithelial-mesenchymal interaction, during alveolarization and for airway branching morphology. (springer.com)
  • Also, these lab grown cells have shown altered gene expression causing a differing phenotype as well as differing antigen levels. (wikipedia.org)
  • Gene therapy has been utilized successfully to repair or inactivate mutations in animal models of monogenic human diseases 19 as well as human in vitro cells 12 . (nature.com)
  • Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. (nih.gov)
  • The matrix is in market exchange format while the row (gene names) and columns (cell barcodes) are provided as TSV files. (nih.gov)
  • The FREM1 gene provides instructions for making a protein that is involved in the formation and organization of basement membranes, which are thin, sheet-like structures that separate and support cells in many tissues. (medlineplus.gov)
  • which means both copies of the gene in each cell have mutations. (medlineplus.gov)
  • The signaling pathways involved in initiating the EMT seem to primarily involve the Smad-dependent pathway, whereby TGFbeta binding to specific high affinity cell surface receptors activates the receptor-Smad/Smad4 complex. (nih.gov)
  • In this review, we cover how the non-epithelial compartment of thymic stroma represent essential cell populations in regulating thymus function. (frontiersin.org)
  • 2003). Similarly, when transplanted into ischemic cortical areas, they were found to differentiate into 'neuron-like' cells (Okawa et al. (scielo.org.ar)
  • 2001) had found amniotic epithelial cells conditioned medium showed neurotrophic effect on rat embryonic day 18 (E18) cortical neurons. (scielo.org.ar)
  • Given our demonstration of the unexpected angiogenic role of AT1 cells and their extensive surface area, we hypothesize that AT1 cells have other novel signaling roles. (mdanderson.org)
  • 2007. Temperature-Dependent Developmental Plasticity of Drosophila Neurons: Cell-Autonomous Roles of Membrane Excitability, Ca Influx, and cAMP Signaling . (truman.edu)
  • What cellular mechanisms drive mammary epithelial invasion and branching? (helsinki.fi)
  • Amniotic epithelial cells were believed to secrete an 'Epidermal Growth Factor (EGF) - like' factor and exact identification was not made. (scielo.org.ar)
  • Compared with normal cells, tumor cells secrete more exosomes, and the number of exosomes secreted by different tumor cells varies dramatically [ 6 ]. (hindawi.com)
  • To enrich for epithelial populations, live, CD140a- cells were collected from E12.5, E14.5, and E17.5 Swiss Webster pancreata. (nih.gov)
  • The expectation is that, once in place, the new RPE cells will support or replace the patient's own failing RPE cells and boost the survival of photoreceptors. (medscape.com)
  • Briefly, eight RNA polymerase I plasmids (for the synthesis of the eight influenza A viral RNAs) together with plasmids for the expression of the PB2, PB1, PA, and NP proteins derived from the influenza A virus strain A/WSN/33 (H1N1) were transfected into 293T cells. (cdc.gov)
  • A series of studies have revealed that exosomes-mediated intercellular transfer of proteins, nucleic acids, and metabolites plays a crucial role in the initiation and progression of HCC, hypoxia and angiogenesis, chemotherapy sensitivity, and cell death mode and regulates the immune microenvironment. (hindawi.com)
  • Regardless of the type or the physiological or pathological state of the originating cell, exosomes share common features as enriched with nucleic acids, lipids, and proteins [ 4 ]. (hindawi.com)
  • Second, mucus and wound-healing proteins (eg, TFF2) and other transcriptional alterations are induced, at which point the reprogrammed chief cells are recognized as mucus-secreting spasmolytic polypeptide-expressing metaplasia cells. (bvsalud.org)
  • METHODS: Primary gastric epithelial cells or fibroblasts from Caviae porcellus were transfected with siRNA IL-33. (bvsalud.org)
  • One possible reason for this is that amniotic epithelial cells have low antigen levels that inhibit compatibility from a donor to the recipient. (wikipedia.org)
  • The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
  • The stage-specific embryonic antigen 4 (SSEA4) is brain. (lu.se)
  • IP: HeLa cells Flow Cyt (intra): HEK293 cells, A-431 cells. (abcam.com)
  • Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. (rndsystems.com)
  • In addition to intrathymic selection, and during this medullary residency period, CD4 + and CD8 + single positive (CD4SP, CD8SP) thymocytes acquire the ability to proliferate in response to TCR stimulation, and undergo licensing for cytokine production, prior to exiting the thymus to join the peripheral T-cell pool as Recent Thymus Emigrants (RTE) ( 5 , 6 ). (frontiersin.org)
  • During EMT, cells will undergo transformation from epithelial phenotype to mesenchymal phenotype ( 14 ) and many characteristics of cells will change including loss of cell-cell adhesion and acquisition of aggressive and metastatic ability ( 15 ). (spandidos-publications.com)
  • Stem cell-like transcriptional reprogramming mediates metastatic resistance to mTOR inhibition. (rochester.edu)
  • An amniotic epithelial cell is a form of stem cell extracted from the lining of the inner membrane of the placenta. (wikipedia.org)
  • Basement membrane (BM) is a sheet-like extracellular matrix that underlies most tissues and acts as a barrier to invading cells. (duke.edu)
  • Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
  • LAPMT5 is a lysosomal membrane protein preferentially expressed in immune cells ( 5 , 6 ) and hematopoietic cells ( 7 ), having a close interaction with the Nedd4 ( 8 ), a member of the E3 ubiquitin ligases family ( 8 ). (spandidos-publications.com)
  • Regulation of cell motility is responsible for these effects. (caltech.edu)
  • Moreover, some studies demonstrated that LAPTM5 was highly expressed in malignant B lymphomas and involved in B cell malignancies ( 10 ), involving in negative regulation of cell surface T and B cell receptor by promoting lysosome degradation ( 6 ). (spandidos-publications.com)
  • not unique for the embryonic system but are shared by other ogenesis regulation must consider different scales of multi-cellular modeling areas. (lu.se)
  • The company won U.S. Food and Drug Administration approval in January to try out human embryonic stem cells for treating macular degeneration , a common cause of vision loss. (foxnews.com)
  • That followed FDA approval in November to test human embryonic stem cells to treat people with a progressive form of blindness called Stargardt's macular dystrophy. (foxnews.com)
  • We hope that these cells will, in the future, provide a treatment not only for these two untreatable diseases -- Stargardt's disease and macular degeneration -- but for patients suffering from a range other debilitating eye diseases. (foxnews.com)
  • Patients had the dry form of age-related macular degeneration (AMD) or Stargardt disease and received injections of human embryonic stem-cell (hESC)-derived RPE cells. (medscape.com)
  • But this is just a first step in the long road towards making regenerative cell therapy a reality in macular and retinal degeneration. (medscape.com)
  • We are also interested in its role in AT1 cell homeostasis and injury in the adult lung. (mdanderson.org)
  • In the adult animal, seam cells are arranged as longitudinal rows of 16 cells on the left and right sides of the body, and they are embedded in the cylindrical hyp 7 syncytium ( SeamFIG 1 ). (wormatlas.org)
  • B. Schematic view of left lateral seam cells in adult hermaphrodite. (wormatlas.org)
  • At L4/adult molt, seam cells fuse to one another making one longitudinal syncytium on each side of the adult animal that extends between the hyp 5 and hyp 7 junction at the anterior and the phasmids at the posterior. (wormatlas.org)
  • B. TEM, transverse section representing an area through the seam-cell body in an adult animal. (wormatlas.org)
  • Stem cells exist both in embryos and adult cells. (articlecity.com)
  • They are able to differentiate into any cell of an organism and have the ability of self-renewal. (articlecity.com)
  • They develop in a juxtanuclear position, adjacent to the centrosome, the microtubule organizing centre of the cell, and share some protein components. (biorxiv.org)
  • Despite the long-standing observation that aggresomes/Lewy bodies and the centrosome sit side-by-side in the cell, no studies have been done to see whether these protein accumulations impede the organelle function. (biorxiv.org)
  • The WT1 protein is a transcription factor predominantly expressed in the embryonic kidneys and gonads. (medscape.com)
  • 293T cells and chicken fibroblast DF-1 cells were maintained in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS). (cdc.gov)
  • Superficial injury heals via surface cells with histopathologic changes like foveolar hyperplasia. (bvsalud.org)
  • To reach these goals, we use a combination of genetic mouse models, single cell and bulk transcriptomics, organ and cell culture methods, and sophisticated time-lapse imaging techniques. (helsinki.fi)
  • Stains mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. (rndsystems.com)
  • Detection of Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Mouse Lgr5/GPR49 and eGFP by Flow Cytometry. (rndsystems.com)
  • HEK293 human embryonic kidney cell line transfected with mouse Lgr5/GPR49 and eGFP was stained with and either (A) Rat Anti-Mouse Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8240) or (B) Rat IgG 2B Isotype Control (Catalog # MAB0061 ) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113 ). (rndsystems.com)
  • Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Mouse Lgf5/GPR49. (rndsystems.com)
  • Lgr5/GPR49 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with GFP (green) tagged mouse LGR5 using Rat Anti-Mouse Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8240) at 10 µg/mL for 3 hours at room temperature. (rndsystems.com)
  • Lgr5/GPR49 in Mouse Cells Transfected with Mouse Lgr5/GPR49. (rndsystems.com)
  • Cells were stained using an anti-Mouse HRP-conjugated secondary antibody (brown). (rndsystems.com)
  • Rat Anti-Mouse Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8240) induces TOPflash activity in the HEK293 human embryonic kidney cell line stably expressing LGR5 in the presence of Wnt-3a, but in the absence of R-Spondins. (rndsystems.com)
  • In contrast, the ED 50 values were much higher in untransformed cells, specifically at ∼8.8 μmol/L in GM05757 (primary normal human fibroblast), ∼8.9 μmol/L in HNEpC (primary normal human nasal epithelial), and ∼19.6 μmol/L in NIH/3T3 (mouse embryonic fibroblast) cells. (aacrjournals.org)
  • ROSA mT/mG mouse embryonic lung imaged using light-sheet microscopy. (elifesciences.org)
  • Denervation of embryonic mouse lung explants reveals that lung branching requires nerves. (caltech.edu)
  • The use of hESC-derived cells is an attractive treatment approach, in fact, for many different diseases because transplanted cells may be able to react to changing conditions in the microenvironment, which is an important biological process, Dr Banin pointed out. (medscape.com)
  • Dr Gregori and her multi-institutional team conducted two phase 1/2 trials using hESC-derived RPE cells, one in dry AMD patients (n = 13) and the other in Stargardt disease (n = 13). (medscape.com)
  • Seam cells are linked to the hypodermis by small adherens junctions along their apical borders and by small gap junctions on their lateral membranes ( SeamFIG 2 ).In a newly hatched animal, there are ten seam cells on each side of the animal (H1-H2, V1-V6 and T). All seam cells, except for H0, divide in a stem-cell-like pattern before each molt. (wormatlas.org)
  • The apical face of each seam cell faces the cuticle and remains in contact with the cuticle along the full length of the cell (a, b). (wormatlas.org)
  • The cells are polarised between an apical and a basal side. (elifesciences.org)
  • Near the apical side, cells adhere tightly via adhesion junctions (green). (elifesciences.org)
  • b ) Apical surface projection of an embryonic lung bud at E12.5 imaged using light-sheet microscopy. (elifesciences.org)
  • Apical and basal epithelial organisation. (elifesciences.org)
  • b ) Apical and basal 2.5D cell segmentation overlays on imaged tip and tube sections (dotted boxes in panel a). (elifesciences.org)
  • c ) Normalised apical and basal cell area distributions in the tip (broken lines) and tube (solid lines) datasets. (elifesciences.org)
  • These transcription factors are the basis of the pluripotency of stem cells. (wikipedia.org)
  • The pluripotency of the human amniotic epithelial cells makes them useful in treating and fighting diseases and disorders of the nervous system as well as other tissues of the human body. (wikipedia.org)
  • However, some studies have shown that once these cells are duplicated in the lab, some of the pluripotency is lost. (wikipedia.org)
  • These cells are known to have some of the same markers as embryonic stem cells, more specifically, Oct-4 and nanog. (wikipedia.org)
  • Amniotic epithelial cells were known to express some of neuronal and glial cell markers (Sakuragawa et al. (scielo.org.ar)
  • 2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)
  • MDCK, 293T, and A549 cells were maintained at 37°C under 5% CO2. (cdc.gov)
  • The pro-regenerative activity of IL-33 was related to stimulation of cells to collagen I production. (bvsalud.org)
  • CONCLUSIONS: The recovery of gastric barrier cells during Hp infection potentially can be affected due to downregulation of pro-regenerative activity of IL-33 by LPS Hp. (bvsalud.org)
  • Since dopaminergic neurons are polarised, ensuring correct localisation of organelles and the effective intracellular transport of neurotransmitter vesicles, loss of centrosome activity could contribute to loss of dopaminergic function and neuronal cell death in Parkinson's disease. (biorxiv.org)
  • In summary, we demonstrate a role of motile cilia in TGF-β1 sensing and showed that TGF-β1 disturbs TJ permeability of conductive airway epithelia by redistributing CLDN3 from TJs into cell nuclei. (springer.com)
  • Therefore, nerves play a conserved role in directing epithelial airway branching. (caltech.edu)
  • While some patients in her study have gained vision, Ninel Z. Gregori, MD, from Bascom Palmer Eye Institute, Miami, Florida, said, "These trials show long term safety and possible biologic activity of pluripotent stem-cell derived progeny, but they were not powered to show efficacy. (medscape.com)
  • In this article, we first provide a short history of cell therapy in Parkinson's disease and briefly describe the current state-of-art regarding human stem cell-derived dopamine neurons for use in any patient trial. (lu.se)
  • A. Schematic view of left lateral seam cells in an L1-stage larva. (wormatlas.org)
  • C. Schematic view of seam cells embedded in the hypodermis, looking from the inside out. (wormatlas.org)