Cell division cycle cell division cycle 2 protein cell cycle. Medical search. Frequent questions

Approximately 20 genes consistently associated with T2DM mainly implicate pancreatic β-cell function in the pathogenesis of T2DM. (scialert.net)
The protein encoded by this gene is a member of the RP/EB family of genes. (nih.gov)
Myb-Related Protein B (MYBL2), a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. (avivasysbio.com)
It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. (avivasysbio.com)
In addition, their osteogenic differentiation potential was increased, and genes involved in cell adhesion, FGF-2 signalling, cell cycle, stemness, cell differentiation, and cell proliferation were upregulated, compared to that of the MSCs cultured on uncoated plates. (hindawi.com)
The cell division cycle of the unicellular eukaryote Trypanosome brucei is tightly regulated despite the paucity of transcriptional control that results from the arrangement of genes in polycistronic units and lack of dynamically regulated transcription factors. (lancs.ac.uk)
The results indicate that TCE induces changes in expression of certain important cell cycle regulation genes. (cdc.gov)

The Cdc7 kinase is involved in regulation of the cell cycle at the point of chromosomal DNA replication. (wikipedia.org)
The Cdc7/Dbf4 complex adds a phosphate group to the minichromosome maintenance (MCM) protein complex allowing for the initiation of DNA replication in mitosis (as explained in the Cdc7 and Replication section below). (wikipedia.org)
It has been suggested that the protein is essential for initiation of DNA replication and that it plays a role in regulating cell cycle progression. (wikipedia.org)
right after replication is over, the protein levels drop. (wikipedia.org)
After chromatin undergoes changes in telophase of mitosis, the hexameric protein complex of MCM proteins 2-7 forms part of the pre-replication complex (pre-RC) by binding to the chromatin and other aiding proteins (Cdc6 and Cdt1). (wikipedia.org)
Since Cdc7 is attached to the Dbf4 protein the entire complex is held in place during replication. (wikipedia.org)
This activation of MCM 2 leads to helicase activity of the MCM complex at the origin of replication. (wikipedia.org)
This is most likely due to the change in conformation allowing the remainder of replication machinery proteins to be loaded. (wikipedia.org)
DNA replication can begin after all the necessary proteins are in place. (wikipedia.org)
The S. cerevisiae ORC binds to specific DNA sequences throughout the cell cycle but becomes active only when it binds to the replication initiator Cdc6. (nature.com)
The ORC-Cdc6 complex (product 1) assembles in step 1 around origin DNA and with the help of another replication initiator protein, Cdt1, it recruits the Mcm2-7 hexamer to the origin in step 2. (nature.com)
Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (6). (cellsignal.com)
Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 7). (cellsignal.com)
Our previous results indicate that 5-ASA improves replication fidelity in colorectal cells, an effect that is active in reducing mutations. (nih.gov)
Interphase is characterized by cell growth and DNA replication , while M is the phase of the cell cycle in which the chromosomes of the cell prepare for the cell to divide into two new cells. (coursehero.com)
With each replication event the amount of dye in the cell is decreased, leading to a characteristic pattern. (bdbiosciences.com)
Mechanisms for maintaining genetic information during cell division and the generation of genetic variation: replication, mitosis, meiosis, recombination. (lu.se)

Chromobox homolog 2 (Cbx2), also known as CDCA6 and M33, is an approximately 56 kDa protein component of the nuclear polycomb repressive complex 1 (PRC1). (rndsystems.com)
The protein localizes to the cytoplasmic microtubule network and binds APCL, a homolog of the adenomatous polyposis coli tumor suppressor gene. (nih.gov)
name: cell division cycle 20 homolog (S. cerevisiae). (jax.org)
names: atonal homolog 1 (Drosophila) and atonal homolog 2 (Drosophila) respectively. (jax.org)

Colchicine prevents the mitosis of the cells at which of the following stage? (zigya.com)
A fifth phase has been identified, G0, in which the cell is resting just after mitosis but still carrying out normal functions. (coursehero.com)
The M phase consists of mitosis, in which the cell divides, producing two new, identical cells. (coursehero.com)
Interphase is collectively the gap 1 (G1), synthesis (S), and gap 2 (G2) phases of mitosis, in which a cell grows, replicates its DNA, and grows again. (coursehero.com)
Other cellular structures are duplicated during G2, such that each replicated daughter cell produced during mitosis will have all necessary organelles (such as mitochondria, endoplasmic reticulum, etc. (coursehero.com)
Mitosis includes prophase, prometaphase, metaphase, and anaphase, as well as telophase, during which chromosome copies are carefully separated in preparation for cytokinesis, where the cytoplasm divides. (coursehero.com)
Mitosis is a form of nuclear division in which replicated chromosomes are carefully organized and separated in preparation for cytoplasmic division. (coursehero.com)
Finally, in the G2 phase, cells continue to grow and prepare for mitosis. (bdbiosciences.com)

Avoid freeze-thaw cycles. (novusbio.com)
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. (rndsystems.com)
For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. (avivasysbio.com)

Trafficking between the nucleus and cytoplasm occurs through nuclear pore complexes (NPCs), which consist of ca. thirty distinct proteins called nucleoporins. (nih.gov)
In interphase cells, GTP-bound Ran (Ran-GTP) is the major form in nucleus while GDP-bound Ran (Ran-GDP) is the predominant form in cytoplasm. (nih.gov)
The asymmetrical distribution of Ran-GTP and Ran-GDP drives cargo transport between the nucleus and cytoplasm through karyopherins, a family of nuclear transport carrier proteins that bind to Ran-GTP. (nih.gov)
During the G2 phase, a structure in the cytoplasm of animal cells that coordinates the formation of microtubules, called a centrosome , allows cell division to proceed during reproduction. (coursehero.com)
These include changes in the plasma membrane (such as loss of membrane symmetry and loss of membrane attachment), a condensation of the cytoplasm and nucleus, protein cleavage, and internucleosomal cleavage of DNA. (bdbiosciences.com)

3. Davidson L, Muniz L, West S. (2014) 3' end formation of pre-mRNA and phosphorylation of Ser2 on the RNA polymerase II CTD are reciprocally coupled in human cells. (guidetopharmacology.org)
Cell cycle progression, phosphorylation, and DNA binding of cell cycle checkpoint proteins were analyzed. (nih.gov)
Chief among the intracellular signals is the phosphorylation of proteins belonging to the pocket protein family, such as p107, p130 and retinoblastoma protein (Rb). (bdbiosciences.com)
A series of signal transduction events starting from the activation of cyclin-dependent kinase 2 (CDK2) to binding of E2F -target transcription to phosphorylation of Rb helps maintain cell-cycle progression. (bdbiosciences.com)
To identify the contribution of dynamic phosphorylation to T. brucei cell cycle control we have combined cell cycle synchronisation by centrifugal elutriation with quantitative phosphoproteomic analysis. (lancs.ac.uk)
Cell cycle regulated changes in phosphorylation site abundance (917 sites, average 5- fold change) were more widespread and of a larger magnitude than changes in protein abundance (443 proteins, average 2-fold change) and were mostly independent of each other. (lancs.ac.uk)
Hierarchical clustering of co-regulated phosphorylation sites according to their cell cycle profile revealed that a bulk increase in phosphorylation occurs across the cell cycle, with a significant enrichment of known cell cycle regulators and RNA binding proteins (RBPs) within the largest clusters. (lancs.ac.uk)
Cell cycle regulated changes in essential cell cycle kinases are temporally co-ordinated with differential phosphorylation of components of the kinetochore and eukaryotic initiation factors, along with many RBPs not previously linked to the cell cycle such as eight PSP1-C terminal domain containing proteins. (lancs.ac.uk)
The temporal profiles demonstrate the importance of dynamic phosphorylation in co-ordinating progression through the cell cycle, and provide evidence that RBPs play a central role in post-transcriptional regulation of the T. brucei cell cycle. (lancs.ac.uk)

It is also important for germ cell viability and meiotic chromosome synapsis. (rndsystems.com)
During S- phase of cell cycle, synthesis of histone proteins takes place as the chromosome number gets doubled to that of somatic number. (zigya.com)
The ultimate goals of our studies are to understand how these proteins enable accurate chromosome segregation and to discover how they are coordinated with each other and with other aspects of cell physiology. (nih.gov)
A centromere , the point on a chromosome that attaches to the spindle fibers with a kinetochore during cell division, attaches the sister chromatids. (coursehero.com)

The product encoded by this gene is predominantly localized in the nucleus and is a cell division cycle protein with kinase activity. (wikipedia.org)
Altered responsiveness to co- oup the nucleus accumbens reflect the behavioral approaches that incorporate caine and increased immobility in the forced swim test associated with elevated cAMP response ele- memory of associations between the extinction-like processes may have effi- ment binding protein in nucleus accumbens. (lu.se)

JAM3 knockdown additionally inhibited trophoblast proliferation and increased the number of trophoblasts in the sub-G1 and G2/M phases, indicating cell-cycle disturbance and apoptosis. (bioone.org)
The SignalStain ® Proliferation/Apoptosis IHC Sampler Kit from Cell Signaling Technology allows the researcher to examine paraffin-embedded tissues or cells with antibodies that will detect cellular apoptosis or proliferation. (cellsignal.com)
Each antibody in the SignalStain ® Proliferation/Apoptosis IHC Sampler Kit detects endogenous levels of its target protein and does not cross-react with any related proteins. (cellsignal.com)
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). (cellsignal.com)
Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle through inhibiting apoptosis and promoting cell division (4,5). (cellsignal.com)
Resveratrol could play a toxic role through inducing apoptosis of the cancer cell in a time- and concentration-dependent manner. (mdpi.com)
Khz inhibited cell division and induced apoptosis in A549 cells. (springer.com)
Induction of apoptosis by ethanol extracts of Ganoderma lucidum in human gastric carcinoma cells. (springer.com)
Ganoderic acid Mf and S induce mitochondria mediated apoptosis in human cervical carcinoma HeLa cells. (springer.com)
Epidemiologic, animal, and laboratory studies suggest that 5-amino-salicylic acid (5-ASA) protects from the development of CRC by altering cell cycle progression and by inducing apoptosis. (nih.gov)
Cells are also programmed for death at the genetic level as a response to DNA damages through the process of apoptosis. (bdbiosciences.com)
2 Apoptosis is triggered by caspase-mediated signal transduction pathways. (bdbiosciences.com)
Cell division, proliferation, and apoptosis and death are integral parts of life. (bdbiosciences.com)
The balance of cell proliferation and apoptosis is important for both development and normal tissue homeostasis. (bdbiosciences.com)
Apoptosis is an organized process that signals cells to self-destruct for cell renewal or to control aberrant cell growth. (bdbiosciences.com)
As cells become damaged or are no longer needed, they undergo apoptosis or programmed cell death, a normal physiological process that occurs during embryonic development and tissue homeostasis. (bdbiosciences.com)
Apoptosis controls the orderly death of damaged cells, whereas necrosis occurs as a result of tissue damage, causing the loss of both damaged and surrounding cells. (bdbiosciences.com)
Methods for detecting apoptosis or dead cells (viability) by cell preparation type. (bdbiosciences.com)
However, some cell types do not display characteristic features of apoptosis. (bdbiosciences.com)
In those cases, multiple aspects of apoptosis might need to be analyzed to confirm the mechanism of cell death. (bdbiosciences.com)

2015) Expression, purification, and identification of associated proteins of the full-length hCDK12/CyclinK complex. (guidetopharmacology.org)

The cell cycle has two major phases: interphase, the phase between mitotic events, and the mitotic phase, where the mother cell divides into two genetically identical daughter cells. (bdbiosciences.com)
Occupational exposure limits with proteins such as topoisomerase inhibitors, and mitotic and meiotic spindle poisons. (cdc.gov)

In this study, we hypothesized that 5-ASA restrains cell cycle progression by activating checkpoint pathways in colorectal cell lines, which would prevent tumor development and improve genomic stability. (nih.gov)
We found that 5-ASA at concentrations between 10 and 40 mmol/L affects cell cycle progression by inducing cells to accumulate in the S phase. (nih.gov)

In mouse embryonic stem cells (ESCs), Cdc7 is needed for proliferation. (wikipedia.org)
Inhibited HER2 activities include downstream signal transduction events and cell cycle regulatory pathways, such as the cell division cycle, ultimately resulting in decreased cell proliferation. (her2support.org)
HER2 positive breast cancer is identified by the overexpression of human epidermal growth factor receptor-2 (HER2) protein in tumor tissue and results in an increased rate of cell proliferation. (her2support.org)
Effects of Ganoderma lucidum spores on HepG2 cells proliferation and growth cycle. (springer.com)
Cell proliferation is an increase in the number of cells as a result of growth and division. (bdbiosciences.com)
A number of techniques are used to assess cell proliferation. (bdbiosciences.com)
Using analogs to these nucleosides provides a way to measure cell proliferation. (bdbiosciences.com)
Several cell cycle-associated proteins, such as Ki-67, are also used as indicators of cell proliferation. (bdbiosciences.com)
Fluorescent or nonfluorescent cytoplasmic proliferation dyes can also be used as a measure cell proliferation. (bdbiosciences.com)
BD Biosciences offers BD Horizon™ Violet Proliferation Dye 450 and BD Horizon™ CFSE for the detection of cell proliferation with the violet laser and blue laser, respectively, which facilitates the use of larger panels. (bdbiosciences.com)

Khz cytotoxicity was measured using an MTT assay and the mitochondrial membrane potential (MMP)-related, calcium-induced generation of reactive oxygen species (ROS) in A549 cells was measured by flow cytometry. (springer.com)
Flow cytometry analysis showed that the percentage of A549 cells in sub-G1 phase of the cell cycle increased in response to Khz treatment. (springer.com)

Mol Cell Biol , 35 (2): 468-78. (guidetopharmacology.org)
Cell Biol. (pdbj.org)

CDK9 promotes RNA synthesis in genetic programs for cell growth, differentiation, and viral pathogenesis. (thermofisher.com)
A cell spends most of its time in interphase, during which it mostly performs its normal functions, such as protein synthesis. (coursehero.com)
Interphase comprises the gap 1 (G1), synthesis (S), and gap 2 (G2) phases, in which the cell grows and replicates its genetic material. (coursehero.com)
When conditions are right, cells enter the S stage of the cell cycle and commit to DNA synthesis and replicate their chromosomal DNA. (bdbiosciences.com)
During the synthesis (S) phase of the cell cycle, DNA polymerases incorporate a variety of nucleosides (deoxyadenosine, deoxyguanosine, deoxycytidine and thymidine) into the newly extending strands of DNA. (bdbiosciences.com)
5-bromo-2'-deoxyuridine (BrdU), a thymidine analog, is widely used to measure de novo DNA synthesis. (bdbiosciences.com)

scaffold_2:1980450. (uu.nl)

During interphase, chromosomes are enclosed within nuclei, and exchange of all molecules between this compartment and the rest of the cell occurs through nuclear pore complexes (NPCs). (nih.gov)

Using this strain, we studied genetic interactions between cell cycle regulators and CDC20 , and the result was highly consistent with the previously identified interactions. (biomedcentral.com)

Our HCC signature covered well-established liver cancer hallmarks, and network analyses revealed coordinated interaction between several MRs. One novel MR, SEC14L2 , exerted an anti-proliferative effect in HCC cells and strongly suppressed tumor growth in a mouse model. (aging-us.com)
Zhao S, Ye G, Fu G, Cheng JX, Yang BB, Peng C. Ganoderma lucidum exerts anti-tumor effects on ovarian cancer cells and enhances their sensitivity to cisplatin. (springer.com)
Studies have shown that transcription factor AP2A2 (activator protein-2 alpha-2) is involved in the expression of DLEC1, a tumor suppressor gene, which, when mutated, will cause breast cancer and is thus an excellent target for breast cancer studies. (bvsalud.org)

organogenesis and somatic embryogenesis, artificial seed, somaclonal variation, secondary metabolism in plant cell culture, embryo culture, in vitro fertilization. (successcds.net)

This study aimed to investigate the anti-proliferative effects of Khz on A549 lung cancer cells. (springer.com)

The protein is a serine-threonine kinase that is activated by another protein called either Dbf4 in the yeast Saccharomyces cerevisiae or ASK in mammals. (wikipedia.org)

Use punctuation only to separate two adjacent numbers (for example, Lamb1-2 ) or for designating related sequences (for example, Emb-rs3 ) and pseudogenes (for example, Adh5-ps1 ). (jax.org)
Bioinformatic analyses of DNA- and protein sequences. (lu.se)

Identifying permissible limits of intracellular parameters such as protein expression provides important information for examining robustness. (biomedcentral.com)
To make the decision of whether to enter the cell cycle or not, cells integrate information from a variety of intracellular and extracellular signals. (bdbiosciences.com)

The expression of p53, Bax, Bcl-2, and actin proteins was analyzed by western blotting. (springer.com)

This Special Issue, "Plant Cell Wall Proteins and Development", will cover a selection of recent research topics in the field of cell wall biology focused on cell wall proteins and their roles during development. (mdpi.com)
5] "Molecular Biology of the cells", B. Alberts et al. (sns.it)
Current Plant Biology 2:1-11. (ugent.be)
Area of expertise: plant molecular cell biology and intercellular signaling in plant reproduction. (jipb.net)
Frontiers in cell and developmental biology. (lu.se)
The course is an optional second-cycle course for a degree of Bachelor or Master of Science in Biology and Molecular Biology. (lu.se)
Gene regulation in developmental biology and the cell cycle. (lu.se)

Cell-cycle arrest was associated with the engagement of checkpoint kinase 2-cell division cycle 25C-cyclin-dependent kinase 1/cyclin B1 signaling. (bioone.org)
Proteins involved in the ATM-and-Rad3-related kinase (ATR)-dependent S-phase checkpoint response (Chk1 and Rad17) were also phosphorylated but not ataxia telengectasia mutated kinase. (nih.gov)
Our data demonstrate that 5-ASA causes cells to reversibly accumulate in S phase and activate an ATR-dependent checkpoint. (nih.gov)

FA is highly reactive towards biomolecules and can react with proteins to form so called "adducts" (Metz et. (cdc.gov)

Cbx2 was detected in immersion fixed iPS2 human induced pluripotent stem cells, differentiated with 0.2 µM retinoic acid for 4 days (panels A and C) or undifferentiated (panels B and D), using Mouse Anti-Human Cbx2 Monoclonal Antibody (Catalog # MAB8098) at 10 µg/mL for 3 hours at room temperature. (rndsystems.com)
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
We show that the S1-Fc fusion protein is extraordinarily immunogenic, as evidenced by robust antibody titers noticed by day 7. (genoimmun.com)
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. (avivasysbio.com)
Immunohistochemical analysis of paraffin embedded Jurkat cell pellets, untreated (left) or etoposide-treated (right), using Phospho-Histone H3 (Ser10) Antibody, PCNA (PC10) Mouse mAb, Cleaved Caspase-3 (Asp175) Antibody and Survivin (71G4B7E) Rabbit mAb. (cellsignal.com)
Monoclonal antibody is produced by immunizing animals with a recombinant Protein A-PCNA fusion protein, or with a synthetic peptide corresponding to residues surrounding Cys60 of human Survivin. (cellsignal.com)

Mechanisms regulating cardiomyocyte cell cycle arrest are of great interest partly because reversing this process could provide a way to stimulate cardiac regeneration after injury [ 17 ]. (springer.com)
In this talk, I will present the work that we have been doing to not only enhance the understanding of molecular mechanisms regulating circadian clock [1] but also to develop therapeutic interventions to modulate the circadian rhythms in mammals [2, 3]. (imsc.res.in)
Pack LR, Daigh LH, Meyer T. Putting the brakes on the cell cycle: mechanisms of cellular growth arrest. (bdbiosciences.com)
Mechanisms that regulate development from single cell to multicellular organisms. (lu.se)

11. Amsel, A. & Roussel, J. Motivational properties of ences in post-transcriptional processes spark interest in the development of frustration: I. Effect on a running response of the (protein redistribution, degradation), pharmacotherapies that selectively reg- addition of frustration to the motivational com- plex. (lu.se)
However, the proteins needed for transcriptional control have been reported to be immunogenic and there is often a substantial leakage in the systems (Clackson, 2000). (lu.se)

Cell division cycle 7-related protein kinase is an enzyme that in humans is encoded by the CDC7 gene. (wikipedia.org)
Much of our current work concerns the RanBP2 complex, which consists of RanBP2 (a large nucleoporin that is also known as Nup358), SUMO-1-conjugated RanGAP1 (the activating protein for the Ran GTPase), and Ubc9 (the conjugating enzyme for the SUMO family of ubiquitin-like modifiers). (nih.gov)

The aim of this review was to discuss the current understanding of Type 2 Diabetes Mellitus (T2DM) genetic advance and aetiology. (scialert.net)
Type 2 Diabetes Mellitus (T2DM) is a complex heterogeneous group of metabolic condition characterized by elevated levels of serum glucose, caused mainly by impairment in both insulin action and insulin secretion. (scialert.net)

Description: Candida Albicans (strain SC5314 / ATCC MYA-2876) Hsp90 co-chaperone Cdc37, recombinant protein. (genoimmun.com)

This internal biological clock regulates a host of cellular responses to the environment, ranging from gene expression and cell division in cyanobacteria, to photosynthesis in plants and finally to the sleep/wake cycles in mammals (commonly referred as circadian rhythms). (imsc.res.in)
Cellular senescence, which is morphologically characterized by an enlarged and flattened cell shape, was first described by Hayflick [ 9 ]. (hindawi.com)
Cellular senescence refers to active cells that eventually enter a state of irreversible growth arrest. (hindawi.com)
Additionally, Abedin and King showed that FGF-2 suppresses the cellular senescence of human MSCs [ 17 ]. (hindawi.com)
If the target protein expression reaches the upper limit, i.e., essential cellular functions come to a halt when the level of protein exceeds a certain limit, the gene/plasmid copy number must decrease to below the upper limit. (biomedcentral.com)
Each of these proteins must have a minimal requirement level (i.e., the lower limit) to support cellular growth under each of these conditions. (biomedcentral.com)

Overexpression of JAM3 or recombinant JAM3 protein enhanced trophoblast adhesion and migration, which were inhibited by JAM3 knockdown. (bioone.org)

During interphase, several kinetochore proteins stably bind to NPCs (e.g. (nih.gov)

Cell wall proteomics, as well as numerous genetic or biochemical studies, have revealed the high diversity of CWPs, among which proteins acting on polysaccharides, proteases, oxido-reductases, lipid-related proteins and structural proteins. (mdpi.com)
The extracellular matrix (ECM) provides structural and biochemical support to the surrounding cells, and it has been used as a coating substrate for cell culture. (hindawi.com)
The ECM provides structural and biochemical support to the cells and has various other functions including cell adhesion, cell to cell communication, and differentiation [ 17 , 18 ]. (hindawi.com)
A collection of microtubules (structural proteins) called a centrosome also replicates. (coursehero.com)

Radial distribution of these molecules against the protein revealed very slight deviation from binding pocket. (bvsalud.org)

Several common birth defects, such as Down's syndrome, result from aneuploidy arising during meiotic cell divisions. (nih.gov)

Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)
The physiology and behavior of almost all living organisms on earth is synchronized to a 24-hour solar cycle by a well-regulated molecular clock mechanism. (imsc.res.in)
2022). Advanced molecular tweezers with lipid anchors against SARS-CoV-2 and other respiratory viruses. (upenn.edu)
Molecular docking studies carried out in Schrodinger with two quinoline derivatives (referred Q1 and Q2) docked against selected target proteins (PDB IDs: 2VAM and1FSE) of B. subtilis demonstrated ideal binding energies (2VAM-Q1: - 4.63 kcal/mol and 2VAM-Q2: - 4.46 kcal/mol, and 1FSE-Q1: - 3.51 kcal/mol, 1FSE-Q2: - 6.34 kcal/mol). (bvsalud.org)

The gene, CDC7, is involved in the regulation of cell cycle because of the gene product Cdc7 kinase. (wikipedia.org)
Because the two proteins, Cdc7 and Dbf4, must form a complex before activating the MCM complex, the regulation of one protein is sufficient for both. (wikipedia.org)
Here we describe a different method of transgene regulation by the use of the human glial ®brillary acidic protein (GFAP) promoter. (lu.se)

To reduce the expression of a gene/protein, we need to reduce the production rate of the transcript, increase the degradation rate of the transcript, decrease the production rate of the protein, or increase the degradation rate of the protein. (biomedcentral.com)

Gene expression patterns in normal human liver cells exposed to tetrachloroethylene using microarray analysis. (cdc.gov)
In this study, we have investigated TCE exposure and global gene expression pattern in normal human liver cells using microarray analysis. (cdc.gov)
Altered gene expression pattems were observed in 194 RNA transcripts with at least a 2 fold change. (cdc.gov)

The G1, S, and G2 phases of the cell cycle are collectively known as interphase. (coursehero.com)
Interphase, the part of the cell cycle between division events, includes the G1, S, and G2 phases, during which the cell grows, replicates its DNA, and undergoes its usual functions. (coursehero.com)
Interphase takes most of the time in the cell cycle, comprising more than 95% of the duration of the cell cycle in most eukaryotes. (coursehero.com)
During interphase, a cell grows larger. (coursehero.com)

Interest in ALMS1 is heightened by the recent discovery of its involvement in neonatal cardiomyocyte cell cycle arrest, a process with potential relevance to regenerative medicine. (springer.com)

Although expression levels of the protein appear to be constant throughout the cell cycle, the protein kinase activity appears to increase during S phase. (wikipedia.org)
This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. (novusbio.com)
JAM3 expression in cell-cell junctions decreased with the formation of syncytiotrophoblasts. (bioone.org)
JAM3 knockdown induced reactive oxygen species and syncytin 2 expression in trophoblasts. (bioone.org)
Furthermore, H 2 O 2 -induced oxidative stress reduced JAM3 expression in trophoblasts and cell culture supernatants. (bioone.org)
Effects of triterpenes from Ganoderma lucidum on protein expression profile of HeLa cells. (springer.com)
In gTOW, we thus increase the expression of a protein to a limit by increasing the copy number and then determine the upper limit by measuring the corresponding copy number of the gene. (biomedcentral.com)
While gTOW is a method to measure the upper limit of protein expression, determination of the lower limit is still a question. (biomedcentral.com)
The commonly used gene-deletion experiments reduce the expression of a target protein to 0. (biomedcentral.com)
At present, there is no effective method to assess the lower expression limit of a target protein. (biomedcentral.com)
To assess the lower limit, we have to gradually reduce the expression of a protein and then measure the lower limit by some methods. (biomedcentral.com)
[ 16 ] In one study, arecoline was found to elevate the mRNA and protein expression of cystatin C, a nonglycosylated basic protein consistently up-regulated in a variety of fibrotic diseases, in a dose-dependent manner in persons with oral submucous fibrosis. (medscape.com)
Expression of the L1 protein using recombinant DNA technology produces noninfectious virus-like particles (VLPs). (cdc.gov)
There are a number of hypothesized that regulating the transgene with a GFAP promoter in a different vectors that transduce cells in the brain in a slightly different viral vector would give rise to a high transgenic expression in the manner (for a review see, e.g. (lu.se)
These vectors have a number of appealing features including the expression by using the machinery of the host cell instead of depending abilities to ef®ciently transduce cells in the central nervous system, on recombinant regulatory proteins. (lu.se)
2000). We of glial cell line-derived neurotrophic factor (GDNF) in models of have recently reported that this vector directs transgene expression to Parkinson's disease (Georgievska et al. (lu.se)
Efforts have therefore, designed a study to compare the expression of green ¯uor- been made to develop systems based on different drugs or hormones escent protein (GFP) and GDNF under the control of the hGFAP including tetracycline, rapamycine and progestrone (Clackson, 2000). (lu.se)

2023). Defining RNA oligonucleotides that reverse deleterious phase transitions of RNA-binding proteins with prion-like domains. (upenn.edu)
19(2): 149-159, 2023. (bvsalud.org)

Poly-L-lysine (PLL) of extracellular matrix proteins is a small natural homopolymer of the essential amino acid L-lysine that is used to coat culture substrates. (hindawi.com)

Unlike embryonic stem cells, adult stem cells (MSCs) have a limited lifespan and stop proliferating during in vitro culture due to replicative senescence [ 8 ]. (hindawi.com)

this means that most eukaryotic cells have the Cdc7 kinase protein. (wikipedia.org)
In eukaryotic cells , DNA is found in structures called chromosomes. (coursehero.com)

View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips . (rndsystems.com)
Hints on fluorescent quantum dots and fluorescent proteins (GFP family). (sns.it)
Fluorescent proteins and their photophysics. (sns.it)
Once the dye is inside the cell, esterases cleave off the ester group to convert the dye into a fluorescent product and trap it inside the cell. (bdbiosciences.com)

Cbx2 in iPS2 Human Stem Cells. (rndsystems.com)
Unless otherwise stated all data on this page refer to the human proteins. (guidetopharmacology.org)
Role of tau N-terminal motif in the secretion of human tau by End Binding proteins. (nih.gov)
Some studies have suggested a role for this protein in maintaining centriole-nucleated sensory organelles termed primary cilia, and AS is now considered to belong to the growing class of human genetic disorders linked to ciliary dysfunction (ciliopathies). (springer.com)
Intended Use Human CDC2L5 ELISA Kit allows for the in vitro quantitative determination of CDC2L5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids. (biobool.com)
MSCs can easily be obtained from various human tissue sources such as the bone marrow, cord blood, placenta, and adipose [ 2 - 5 ]. (hindawi.com)
RT "A human phosphatidylinositol 3-kinase complex related to the yeast Vps34p- RT Vps15p protein sorting system. (pdbj.org)
Human immunodeficiency virus (HIV) infection results from 1 of 2 similar retroviruses (HIV-1 and HIV-2) that destroy CD4+ lymphocytes and impair cell-mediated immunity, increasing risk of certain infections and cancers. (msdmanuals.com)
Human Immunodeficiency Virus (HIV) Infection in Infants and Children Human immunodeficiency virus (HIV) infection is caused by the retrovirus HIV-1 (and less commonly by the related retrovirus HIV-2). (msdmanuals.com)

Jacobson MD. Reactive oxygen species and programmed cell death. (springer.com)
Miyajima, A, Nakashima J, Yoshioka K. Role of reactive oxygen species in cis-dichlorodiammineplatium-induced cytotoxicity on bladder cancer cells. (springer.com)

Using hybrid/integrative modeling, involving multiple experimental and computational methods, we have been able provide mechanistic insights into the role of cryptochromes a core clock protein, in regulating circadian period length, thus directly relating protein structure and dynamics to in vitro and in vivo experimental observations [1]. (imsc.res.in)
However, MSCs need to be expanded in vitro in order to obtain sufficient cells for clinical trials since they are extremely rare in various tissues. (hindawi.com)
Although other irritants in the smoke may have contributed to the incident, there is supporting evidence that stable strontium can stimulate the release of histamine from mast cells in vitro (ATSDR 2001e). (cdc.gov)

CDK9 (cyclin-dependent kinase 9) is a member of the cyclin-dependent protein kinase family. (thermofisher.com)
The encoded protein is phosphorylated by cyclin A/cyclin-dependent kinase 2 during the S-phase of the cell cycle and possesses both activator and repressor activities. (avivasysbio.com)

Anatomy31

Organisms19

Diseases2

Chemicals and Drugs99

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes84

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science5

Health Care2

Genes7

Replication17

Homolog4

Mitosis8

Freeze-thaw cycles3

Cytoplasm5

Phosphorylation9

Chromosome4

Nucleus2

Apoptosis20

Purification1

Mitotic2

Progression2

Proliferation10

Flow cytometry2

Biol2

Synthesis6

Scaffold1

Nuclear1

CDC201

Tumor3

Metabolism1

Anti-proliferative1

Serine1

Sequences2

Intracellular2

Actin1

Biology7

Checkpoint3

Biomolecules1

Antibody6

Mechanisms4

Transcriptional2

Enzyme2

Type 2 Diabet2

Recombinant Protein1

Cellular6

Overexpression1

Kinetochore1

Structural4

Molecules1

Meiotic1

Molecular4

Regulation3

Transcript1

Gene expression3

Interphase4

Arrest1

Expression17

20232

Extracellular1

Embryonic stem1

Eukaryotic cells2

Fluorescent4

Human9

Reactive2

Vitro3

Cyclin depend2