• Stem cell- and tissue- derived organoids have more complex 3D tissue-like architecture and structure. (sigmaaldrich.com)
  • Dispase is suitable for tissue disaggregation and subcultivation procedures since it does not damage cell membranes. (thomassci.com)
  • Neutral protease (Dispase®) is designed for primary cell isolation and tissue dissociation. (thomassci.com)
  • Next, transfer the tissue fragments into a single cell suspension dissociation tube containing the digestion mix and tightly close the tube. (jove.com)
  • Cell counters frequently exhibit problems in handling heterogeneous samples after tissue dissociation. (jove.com)
  • Schneider's Drosophila Powder Medium, enzymes and reagents for cell and tissue dissociation, media supplements, growth factors and extracellular matrices - SERVA's products for cell culture provide the quality and consistency you need and ensure reproducible results and successful research. (serva.de)
  • Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? (hpscreg.eu)
  • It is a ready-to-use non-mammalian, non-bacterial replacement for all applications of trypsin and collagenase in tissue dissociation, cell counting, and dissolving cell clumps, such as spheroids. (diagnovum.com)
  • Cell lysis only takes a few minutes per well, but tissue homogenisation can take 10-20 minutes per sample depending on how tough the tissue is. (openwetware.org)
  • This reagent does NOT contain porcine trypsin or any other protein. (fishersci.com)
  • In fact, it works as quickly as trypsin on most cells. (fishersci.com)
  • Trypsin solutions are widely utilized as cell dissociation reagents for continuous cell culture of adherent growing cells. (bioind.com)
  • EDTA is a chelator that binds calcium and magnesium ions that may otherwise inhibit the trypsin activity, which then hydrolyzes and gains access to the intercellular bonds (cell-cell and/or cell-substrate bonds). (bioind.com)
  • Biological Industries' Trypsin is designed to gently dissociate cells from almost any support substrates, as well as from each other, in order to actualize cell manipulation techniques, and for other studies that require intact cell-surface proteins. (bioind.com)
  • Trypsin and alternative cell dissociation products are ideal for use with tissues and cell monolayers. (gaiascience.com.my)
  • Trypsin reagent comes in a wide variety of formats to meet the diverse needs of researchers performing adherent cell culture. (gaiascience.com.my)
  • TrypRC™ is a recombinant enzyme alternative to animal trypsin for the dissociation of adherent cell lines from plasticware. (gaiascience.com.my)
  • Our product portfolio includes a comprehensive line of fetal bovine serum (FBS), human serum (HSAB), other animal sera, cell culture media, supplements and reagents, growth factors and cytokines, plus lab consumables. (geminibio.com)
  • Additionally, advancements in non-enzymatic dissociation approaches are expected to offer lucrative opportunities for the end users of this market. (marketsandmarkets.com)
  • Most researchers in R&D institutes and biotechnology companies have primarily relied on traditional enzymatic dissociation products to separate cells from primary tissues. (marketsandmarkets.com)
  • Although these traditional enzymatic dissociation products have advantages, such as better dissociation while performing research, they can sometimes be cytotoxic and damage viable cells. (marketsandmarkets.com)
  • Owing to this advantage, the demand for non-enzymatic dissociation products is increasing among several end users. (marketsandmarkets.com)
  • Based on the product, the cell dissociation market is segmented into non-enzymatic dissociation products, enzymatic dissociation products and instruments & accessories. (marketsandmarkets.com)
  • Collagenase segment is the key contributor to the dominance of enzymatic dissociation products. (marketsandmarkets.com)
  • Dissocation of pluripotent stem cell (PSCs) can be achieved by different methods, including the use of either enzymatic or non-enzymatic reagents. (thermofisher.com)
  • Use Vitronectin XF™ with mTeSR™1 , mTeSR™ Plus , TeSR™-E8™ , or TeSR™-AOF medium to provide a defined culture system for the maintenance of human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells under feeder-free conditions. (stemcell.com)
  • Astrocytes are generated from neural stem cells (NSCs) during late embryonic and early postnatal stages. (nature.com)
  • culture and passage of human embryonic and induced pluripotent stem cells.Dispase-II enzyme is produced in Bacillus polymyxa. (thomassci.com)
  • For Swiss Webster embryonic pancreatic dissociations, cells were also stained with the mesenchymal marker, CD140a. (nih.gov)
  • We describe a two-step differentiation protocol that starts with differentiation of H1 human embryonic stem cells (hESCs) into embryoid bodies (EBs) in mesoderm-inducing conditions followed by maturation of EB derived CD34+ cells on OP9M2 stroma on HSC supportive conditions. (researchsquare.com)
  • Embryonic stem cells (ESC) can be expanded indefinitely without undergoing replicative senescence or aging due to their high telomerase expression. (biomedcentral.com)
  • Key driving factors of the market include an increase in demand for personalized medicine, cGMP approvals each year for new production facilities developing cell therapies, and the growing use of cell dissociation products in mammalian cell culture to develop recombinant therapeutics. (marketsandmarkets.com)
  • We support the unique needs of plasmid DNA and viral vector manufacturing, as well as therapeutic cell manufacturing, by providing custom manufactured cGMP media, buffers, and other process liquids in batch sizes from 10-liters to 10,000-liters. (geminibio.com)
  • EZ-LiFT does not suffer from the drawbacks of other enzyme-based dissociation reagents such as Dispase, Collagenase IV and Accutase. (thomassci.com)
  • More than 20 different types of collagenases exist, but only a handful are most suited for the enzyme dissociation of tissues. (marketsandmarkets.com)
  • It can be used to dissociate primary cells, tissues, and tumors with an increased plating efficiency. (fishersci.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Next, mix 20 microliters of magnetic labeling reagent for mouse cells into the cell suspension and incubate the cells for 15 minutes in a refrigerator. (jove.com)
  • Most importantly, it preserves the structural and functional integrity of cell surface proteins and does not have the cytotoxic effects sometimes associated with 0.5mm EDTA. (fishersci.com)
  • SPGA-EDTA Solution is a suspending medium utilized for the extraction of cell-free viruses. (bioind.com)
  • Dissociations were neutralized with FACS buffer (10% FBS + 2mM EDTA in phenol-red free HBSS). (nih.gov)
  • EDTA-metal complexes are very stable and have high dissociation constants. (dojindo.com)
  • For these reasons, EDTA is a widely used titration reagent. (dojindo.com)
  • These polymer-nucleotide substrates, termed avidites, decrease the required concentration of reporting nucleotides from micromolar to nanomolar and yield negligible dissociation rates. (rna-seqblog.com)
  • A reagent containing multivalent avidite substrates and an engineered polymerase are combined with DNA polonies inside a flowcell. (rna-seqblog.com)
  • It was during this time that I developed a passion for studying cancer stem cell biology and the molecular signaling that promotes glioblastoma (GBM) tumor progression. (labmanager.com)
  • As the former director of the Starr Foundation Cerebral Organoid Translational Core at Weill Cornell Medicine, I established a novel, ex vivo 3D system to study the interactions and molecular crosstalk between brain tumor cells and a miniature model of the human brain. (labmanager.com)
  • Divalent cations and group-specific chemical modifiers were used to modify sodium efflux in order to probe the molecular structure of sodium channels in dog red blood cells. (cdc.gov)
  • This protocol generates cells that resemble human foetal liver hematopoietic stem/progenitor cells (HSPC) by immunophenotype (CD34+CD38loCD90+CD45+GPI80+/-) and molecular profile. (researchsquare.com)
  • Here, we describe a two-step differentiation protocol for H1 hESC that combines 2 weeks of embryoid body differentiation in mesoderm inducing conditions followed by OP9M2 stroma co-culture in HSC supportive conditions, which enables the generation of hematopoietic cells that resemble foetal liver HSPCs by immunophenotype (CD34+CD38loCD90+CD45+GPI80+) 2 and molecular profile. (researchsquare.com)
  • The sequence of affinities, as measured by the dissociation constants, reflects the reactivity of these divalent cations with sulfhydryl groups. (cdc.gov)
  • Their selectivity depends on the dissociation constants of metal ions and their sensitivity depends on the molar absorptivity of the complex. (dojindo.com)
  • Isolation and purification of cell organelles integrates expensive products, additionally, automated/sem-automated benchtop instruments are more expensive than traditional methods. (marketsandmarkets.com)
  • Here, we establish a method to enable clonal isolation, selection, and expansion of properly edited cells, using OptoElectroPositioning technology for single-cell manipulation on a nanofluidic device. (nature.com)
  • Another experimental challenge that we faced included isolation of tumor cells from the normal 3D mini-brain. (labmanager.com)
  • It is commonly used to separate skin epidermis from dermis leaving intact epithelial sheets and stem cell, hepatocyte and other cell isolation applications. (thomassci.com)
  • There, a viral reverse transcriptase enzyme adds DNA nucleotides onto the RNA sequence, generating cDNA that is inserted into the host cell genome by the enzyme integrase, encoding viral proteins. (wikipedia.org)
  • Enzyme-Free Cell Dissociation Solution is a Hank's based formulation of chelating agents and agents to stabilize their activity on cells. (fishersci.com)
  • Applying MPTR to dermal fibroblasts from middle-aged donors, we found that cells temporarily lose and then reacquire their fibroblast identity, possibly as a result of epigenetic memory at enhancers and/or persistent expression of some fibroblast genes. (elifesciences.org)
  • We have determined the dissociation rates of Arp2/3 and CP as 0.048 and 0.58 s −1 , respectively, in lamellipodia of live XTC fibroblasts. (silverchair.com)
  • We followed the quantification steps of the qPCR reactions with a dissociation assay and ran both in a plate format on an ABI 7500 qPCR thermal cycler. (cdc.gov)
  • Cell viability assay also revealed that the extracts could safely be used in concentrations of 125 µg/mL. (bvsalud.org)
  • The key limitation of dissociated cultures is the small number of cells relative to immortalized cell lines. (marketsandmarkets.com)
  • Additionally, most primary cell cultures are not homogeneous, which poses a challenging scenario for cell dissociation experiments. (marketsandmarkets.com)
  • Neuronal cultures are often mixtures of both glia and neurons that respond to different neurotransmitters, so identifying an individual population of cells can be difficult. (marketsandmarkets.com)
  • Dipase is an extremely stable microbial metalloprotease suitable for use in cell cultures. (thomassci.com)
  • from Clostridium histolyticum and Bacillus polymyxa cultures devoid of animal containing reagents. (thomassci.com)
  • Herein, we present the first evaluation of cationic dendrimers based on 2,2-bis(methylol)propionic acid (bis-MPA) as nonviral vectors for transfection of short interfering RNA (siRNA) in cell cultures. (mdpi.com)
  • We demonstrate here that highly sensitive in vitro bioassays for FSH, TSH, and PTH can be set up in mouse Leydig Tumor Cells (mLTC), in addition to the normal LH/CG bioassay, after they were transfected with expression vectors encoding the corresponding Gs Protein-Coupled Receptors (GsPCR), such as FSHR, TSHR, or PTHR. (bvsalud.org)
  • These varying degress of dissociation, from larger clumps to clusters of 2-3 cells or single cells, can be accomplished using different passaging reagents. (thermofisher.com)
  • Moreover, even when high-purity single-cell sorting can be achieved, viability after sorting is often low to moderate, especially for cell types that are particularly sensitive to hydrodynamic stress or low-density culture conditions (e.g., primary cells or pluripotent stem cell lines). (nature.com)
  • Generation of patient-derived pluripotent stem cell-lines and CRISPR modified isogenic controls with mutations in the Parkinson's associated GBA gene. (hpscreg.eu)
  • The biopharmaceutical industry has witnessed a considerable increase in the proportion of recombinant therapeutics sourced from mammalian cells since 2002. (marketsandmarkets.com)
  • The number of approved recombinant products from mammalian cell culture increased by 8.5% annually from 2002 to 2022. (marketsandmarkets.com)
  • Cell culture products, including cell dissociation form an integral part of developing mammalian-based recombinant therapeutics, propelling the industry growth. (marketsandmarkets.com)
  • We report that the variable domains of recombinant HIV-1 Env immunogens activate a large number of B cell clones that give rise to many non-neutralizing antibodies, and that removing the variable domains from the immunogen reduces the number of activated B cell lineages and leads to the development of autologous neutralizing antibodies, a step towards bNAb-production. (plos.org)
  • 1 Monolayers of Caco‐2 cells, a transformed cell line derived from human colorectal carcinoma, have been widely used by pharmaceutical companies as a standard intestinal barrier model for the prediction of intestinal permeability for drug candidates. (sigmaaldrich.com)
  • Amanda Linkous , PhD, research associate professor, Department of Biochemistry and scientific center manager, NCI Center for Systems Biology of Small Cell Lung Cancer at Vanderbilt University , talks to contributing editor Tanuja Koppal, PhD, about new scientific and technological advancements in 3D cell culture. (labmanager.com)
  • Building off experience in hematopoietic stem cell transplant and exploiting advances in genetic engineering, genome editing, immunology, immunotherapy, stem cell biology, understanding disease mechanisms and cell therapy manufacturing, considerable progress has been made with hundreds of clinical trials underway. (bdbiosciences.com)
  • Dispense 100 µL/well CellTiter-Glo ® 3D Cell Viability Reagent. (sigmaaldrich.com)
  • Cytotoxic effects of five compounds (flavopiridol, loperamide, paracetamol, ketoprofen and alosetron) on human colon organoids using the CellTiter-Glo ® 3D Cell Viability Reagent. (sigmaaldrich.com)
  • Cell viability of five compounds were tested: two compounds (flavopiridol and loperamide) had negative effects on cell viability, three compounds (paracetamol, ketoprofen, alosetron) had no effects on cell viability. (sigmaaldrich.com)
  • Here we demonstrate the use of highly-characterized human iPS cell-derived colon organoids as an alternative cell model for traditional Caco-2 cell drug toxicity assays for drug screening applications. (sigmaaldrich.com)
  • Neural Progenitor Cells Derived from XCL-1 DCXp-GFP are a neuronal lineage reporter cell line that can be used in neuronal differentiation and drug screening. (atcc.org)
  • When a single cell suspension has been obtained, quickly centrifuge the tube to collect the cells at the bottom of the tube. (jove.com)
  • Then centrifuge the cells and resuspend the human tumor mouse stromal cell pellet in five milliliters of PB buffer. (jove.com)
  • Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. (cytion.com)
  • Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). (cytion.com)
  • To directly differentiate to pancreatic cells, use our pancreatic progenitor kit to generate functional PDX1 + /NKX6.1 + cells that can be matured into insulin-producing beta cells. (stemcell.com)
  • CP dissociates slower from actin stress fibers than from the lamellipodial actin network, suggesting that CP dissociation correlates with actin filament dynamics. (silverchair.com)
  • Hg++, Ni++, Co++, and PCMBS (parachloromercuribenzene sulfonic acid), a sulfhydryl reactive reagent, induce large increases in Na+ permeability and their effects can be described by a curve which assumes 2:1 binding with the sodium channel. (cdc.gov)
  • Fluoxetine (FLX), a selective serotonin reuptake inhibitor antidepressant, exhibits various other mechanisms of action in numerous cell types and has been shown to induce cell death in cancer cells, paving the way for its potential use in cancer therapy. (bvsalud.org)
  • Founded in 1985, GeminiBio serves the global biotechnology industry, from basic research to commercial production, with a focus on helping our customers accelerate the development of life-enhancing biotherapeutics by streamlining and improving their cell culture and process liquid manufacturing workflows. (geminibio.com)
  • GeminiBio enables cell therapy customers to optimize their upstream and downstream workflows to improve productivity and speed. (geminibio.com)
  • Different workflows, including routine passaging, gene editing and stem cell differentiation, require varying degrees of dissociation. (thermofisher.com)
  • Differentiate human pluripotent stem cells (hPSCs) to cells of the endoderm lineage with our specialized cell culture media. (stemcell.com)
  • Support the growth and differentiation of human pluripotent stem cells by using Vitronectin XF™, a defined and xeno-free cell culture matrix. (stemcell.com)
  • This system allows complete control over the culture environment, resulting in more consistent cell populations and reproducible results in downstream applications. (stemcell.com)
  • Investigating astrocyte maturation in a cell culture model revealed that in vitro-differentiated astrocytes lack expression of many mature astrocyte-specific genes, including genes for the transcription factors Rorb, Dbx2, Lhx2 and Fezf2. (nature.com)
  • CAR) T cells are then grown and expanded in defined cell culture conditions and harvested. (bdbiosciences.com)
  • GeminiBio's products are organized into two core verticals - Cell Culture Solutions and Process Liquid Solutions. (geminibio.com)
  • GeminiBio enables researchers to optimize their cell culture workflow to improve productivity, reproducibility, and speed. (geminibio.com)
  • Any of the biochemicals that are regularly used by Biological Industries in the manufacture of their cell culture products are available to be purchased separately. (cellseco.com)
  • Cell culture was established from the left tumor which showed fetal rhabdomyomatous histology as described by Royer-Pokora 2010. (cytion.com)
  • In vitro cell culture model. (cytion.com)
  • Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). (cytion.com)
  • Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. (cytion.com)
  • Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. (cytion.com)
  • One or two cell culture flasks come filled with cell culture medium. (cytion.com)
  • Carefully add 5 ml of cell culture medium to each T25 cell culture flask. (cytion.com)
  • If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. (cytion.com)
  • While the embryoid body differentiation generates immature haematovascular precursors, co-culture on mouse bone marrow OP9M2 stroma 3 enables subsequent maturation step of the CD34+ haemato-vascular cells to immunophenotypic human HSPCs. (researchsquare.com)
  • We show that treatment of EB-derived CD34+ cells with a pulse of retinoic acid receptor alpha agonist (AM580) during the first days of stroma co-culture promotes the definitive HSC fate by inducing the expression of developmental regulators of definitive HSC genetic program, while suppressing genes associated with differentiation primed progenitors. (researchsquare.com)
  • 3 Because this bone cell culture model demonstrates a capacity to respond to mechanical changes, we hypothesize that this model may also show differences in expression of metalloproteinases in response to cyclic mechanical stretch that can be used to model changes at the bone-implant interface. (allenpress.com)
  • We assessed their cytotoxic effect after sensitizing cancer cells with insulin. (oncotarget.com)
  • The resultant activation of T cells is then exploited for tumor elimination. (bdbiosciences.com)
  • Patient PBMCs are collected by leukapheresis followed by enrichment approaches to isolate T cells while removing other cells such as B cells, monocytes, neutrophils, dendritic cells and contaminating tumor cells. (bdbiosciences.com)
  • The fourth generation of CAR-T design attempts to deliver cytokines to modulate the tumor microenvironment either by activating host effector T cells or hampering host suppressors and reinforcing memory T cells. (bdbiosciences.com)
  • Human tumor xenografts are vascularized and infiltrated by cells of mouse origin during the growth phase in vivo . (jove.com)
  • The overall goal of this procedure is to rapidly isolate xenotransplanted human tumor cells from mice to improve the downstream analysis of the desired target cells. (jove.com)
  • The elimination of the contuminating mouse cells by this method allows the specific assessment of human tumor cell gene expression or in vitro direct responses. (jove.com)
  • The main advantage of this technique is that the human tumor cells can be quickly and easily isolated without having to identify any surface markers on the target cells. (jove.com)
  • Attach the heater to the dissociator, then click the folder symbol on the touch screen and use the up and down arrows to select the appropriate tumor dissociation mode. (jove.com)
  • The aim of this study was to determine the off-target effects of the anti-depressant drug FLX, on the human ovarian granulosa tumor COV434 cells stimulated by forskolin (FSK), by measuring the real-time kinetics of intracellular cyclic AMP (cAMP), ATP level, cytoplasmic calcium ([Ca2+]cyt) and survival of COV434 cells. (bvsalud.org)
  • To download a certificate of analysis for Neural Progenitor Cells Derived from XCL-1 DCXp-GFP ( ACS-5005 ), enter the lot number exactly as it appears on your product label or packing slip. (atcc.org)
  • The certificate of analysis for that lot of Neural Progenitor Cells Derived from XCL-1 DCXp-GFP ( ACS-5005 ) is not currently available online. (atcc.org)
  • Organoids, termed "mini-organs" by many, are comprised of organ-specific cell types (from stem cells or progenitor cells) that exhibit lineage-specific differentiation and self-assembly. (labmanager.com)
  • BD Biosciences supports each stage of this journey, with research tools for single-cell multiomics, high-parameter analysis and informatics to deeply characterize cell samples in discovery. (bdbiosciences.com)
  • Colorimetric chelating reagents form colored complexes with metal ions in pH-controlled solutions. (dojindo.com)
  • In vivo DNA synthesis (DNA replication) is dependent on a complex set of enzymes which have evolved to act during the S phase of the cell cycle, in a concerted fashion. (wikipedia.org)
  • AccuMax is a cell dissociation solution containing proteolytic, collagenolytic, and DNase enzymes. (diagnovum.com)
  • See More Use our definitive endoderm (DE) differentiation kit to obtain functional DE cells that are capable of downstream differentiation to hepatic, intestinal, pancreatic and pulmonary cells. (stemcell.com)
  • The main property that sets ESC apart from other cells is their pluripotent nature, meaning they can give rise/differentiate to cells of the three primary germ layers (mesoderm, endoderm and ectoderm) [ 13 ]. (biomedcentral.com)
  • CAR-T cells, which incorporate an antibody-derived extracellular receptor and T cell derived intracellular signaling domains, have shown convincing outcomes in certain types of leukemia and lymphoma, including commercial licensure of CD19 CAR-Ts for the treatment of relapsed/ refractory large B cell lymphoma and acute lymphoblastic leukemia. (bdbiosciences.com)
  • Stem cells rely on extracellular signals produced by the niche, which dictate their ability to self-renew, expand and differentiate. (karger.com)
  • AccuMax is a cell detachment solution suitable for various cell types. (diagnovum.com)
  • Patient-derived glioma stem cells (green) invading and proliferating within a miniature replica of the human brain. (labmanager.com)
  • It is essential to have sensitive and reproducible methods of either quantifying or isolating these stem cells and progenitors to understand their intrinsic properties and how extrinsic signals regulate their development. (karger.com)
  • However, stem cells are difficult to distinguish from multipotential progenitors, which may look and act like them. (karger.com)
  • JangoCell offers a wide selection of canine mesenchymal stem cells for sale, including dog adipose-derived stem cells (ADSC), dog bone marrow stem cells (BM-MSCs), dog umbilical cord stem cells (UC-MSCs), and fresh or frozen dog PBMC and dog T cells. (jangocell.com)
  • The generation of definitive, transplantable haematopoietic stem cells (HSC) from pluripotent stem cells (PSC) is an unmet challenge. (researchsquare.com)
  • The attempts to generate definitive, transplantable hematopoietic stem cells (HSC) from human pluripotent stem cells have not been successful. (researchsquare.com)
  • It has been proposed that hematopoietic differentiation from pluripotent stem cells is biased toward the generation of the yolk sac like differentiation-primed progenitors. (researchsquare.com)
  • Induced pluripotent stem cells (iPS) can be generated from various somatic cells and can subsequently be differentiated to multiple cell types of the body. (biomedcentral.com)
  • Cell engineering through gene editing is fundamentally a two-step bioprocess. (nature.com)
  • We have identified a novel gene, gtcA , involved in the decoration of cell wall teichoic acid of Listeria monocytogenes serotype 4b with galactose and glucose. (asm.org)
  • The matrix is in market exchange format while the row (gene names) and columns (cell barcodes) are provided as TSV files. (nih.gov)
  • These experimental cytokine-producing CAR-Ts, called T cells redirected for universal cytokine killing (TRUCKs), can deliver a variety of cytokines, such as IL-12, IL-15, IL-18 or IL-21. (bdbiosciences.com)
  • Phenotypic consequences of editing are rapidly assessed on-chip with cell-surface staining for CXCR4. (nature.com)
  • The NP-EGTA salt can be complexed with Ca 2+ to generate a caged Ca 2+ reagent that will rapidly deliver Ca 2+ upon photolysis ( Figure 19.8.1 ). (thermofisher.com)
  • Solid tumors, autoimmune diseases, graft versus host disease (GvHD), genetic diseases and neurodegenerative diseases are investigated using many types of autologous and allogeneic cells with rapidly evolving cell engineering and editing approaches. (bdbiosciences.com)
  • Altogether our FACS (fluorescence-activated cell sorter) analyses reveal that the neonatal subventricular zone is far more heterogeneous than previously suspected and our studies provide new insights into the signals and mechanisms that regulate their self-renewal and proliferation. (karger.com)
  • In the present study, our aim was to establish an in vitro cell model based on patient-specific human neurons to study the pathomechanism of sporadic AD. (biomedcentral.com)
  • These cells have the ability to generate progeny associated with definitive haematopoiesis (adult globin expressing erythroid cells and T-lymphocytes) but have limited expansion ability in vitro and cannot engraft in vivo. (researchsquare.com)
  • This CP dissociation rate is three orders of magnitude faster than in vitro. (silverchair.com)
  • The global cell dissociation market in terms of revenue was estimated to be worth $0.6 billion in 2023 and is poised to reach $1.4 billion by 2028, growing at a CAGR of 17.8% from 2023 to 2028. (marketsandmarkets.com)
  • GeminiBio supports therapeutic cell manufacturing with a comprehensive line of human serum products, and mesenchymal stem cell (MSC) and T-Cell media. (geminibio.com)
  • Split organoids using the 3dGRO™ Organoid Dissociation Reagent ( SCM300 ) following recommended instructions. (sigmaaldrich.com)
  • Our contribution is to use a buffer solution, or reagent, that we all consume regularly in various foods. (foxcroft.com)
  • Sodium permeability of dog red blood cell membranes. (cdc.gov)
  • It is concluded that the movement of sodium ions through ionic channels in dog red blood cells can be enhanced by modification of amino and sulfhydryl groups. (cdc.gov)
  • Although previous protocols have generated clonogenic progenitors and various differentiated hematopoietic cells, there is no confirmation of their definitive identity or functional HSC properties. (researchsquare.com)
  • Many protocols have been described that achieve the generation of clonogenic progenitors and various differentiated hematopoietic cells. (researchsquare.com)
  • Recent work has demonstrated that the epigenome is already rejuvenated by the maturation phase of somatic cell reprogramming, which suggests full reprogramming is not required to reverse ageing of somatic cells. (elifesciences.org)
  • If one administers the appropriate growth factor or biological cue, but at the inappropriate time, then there can be wide variation in the resulting cell types that are generated. (labmanager.com)