AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation ScienceNamed Groups
Cell DifferentiationHematopoietic Stem CellsHematopoietic Stem Cell TransplantationStem CellsCell LineageHematopoiesisCells, CulturedMice, Inbred C57BLBone Marrow CellsAntigens, CD34Cell ProliferationGene Expression Regulation, DevelopmentalSignal TransductionTranscription FactorsHematopoietic Stem Cell MobilizationFlow CytometryStem Cell TransplantationCell LineGene Expression RegulationCell DivisionStem Cell NicheTransplantation, HomologousEmbryonic Stem CellsColony-Forming Units AssayMice, KnockoutAdult Stem CellsRNA, MessengerMice, TransgenicNeoplastic Stem CellsMesenchymal Stromal CellsReverse Transcriptase Polymerase Chain ReactionPluripotent Stem CellsBone MarrowFetal BloodHomeodomain ProteinsMultipotent Stem CellsPhenotypeDNA-Binding ProteinsCell SeparationTretinoinMolecular Sequence DataStem Cell FactorGene ExpressionPlant StemsBase SequenceEmbryo, MammalianHematopoietic SystemNeural Stem CellsTime FactorsCell Culture TechniquesGene Expression ProfilingMice, SCIDOsteoblastsMesonephrosImmunohistochemistryGraft vs Host DiseaseModels, BiologicalCell CycleAntigens, DifferentiationAntigens, CDSex DifferentiationBone Marrow TransplantationTransplantation ConditioningB-LymphocytesBasic Helix-Loop-Helix Transcription FactorsOsteogenesisCoculture TechniquesTranscription, GeneticMyeloid Progenitor CellsCell SurvivalReceptors, NotchProto-Oncogene Proteins c-kitFetal Stem CellsInduced Pluripotent Stem CellsTrans-ActivatorsLymphopoiesisStromal CellsMice, Inbred NODLeukemiaHematologic NeoplasmsTransfectionRepressor ProteinsPromoter Regions, GeneticLentivirusApoptosisGranulocytesBiological MarkersCell MovementDown-RegulationMembrane ProteinsTransduction, GeneticBlotting, WesternNuclear ProteinsTransplantation, AutologousMutationTumor Cells, CulturedT-LymphocytesGenetic VectorsMice, Mutant StrainsIn Situ Hybridization
Proliferation and differentiationHematopoiesisBone marrow hematopoietic stemEmbryonic stemTransplantationProgenitorsLineagesSomaticSelf-renewal and differentiationPrecursorsIPSCMesenchymalMyeloidRegulator of hematopoieticRegulatesCharacterizationTissuesVitro differentiationMaturationDifferentiate2020HSCsNeuronalProgenitor cellPluripotent stemMalignant hematopoieticIPSCsHSCTClonalAntigensMolecularGenesDental pulp stem cellsLeukemiaCellularVivoDisordersTumorImmatureGene expressionImmuneInhibition of differentiationRegenerationOsteogenicBiologyInduceTherapeuticMethodsPhenotypesErythroidProteinsCRISPRStromal cellsMutationsCytokinesErythropoiesis
Proliferation and differentiation6
  • In 2017, the research team used the Tianzhou-1 cargo spacecraft to conduct research on the proliferation and differentiation of embryonic stem cells on a mouse. (pdnews.cn)
  • Furthermore, the study examined the effects of cooperating oncoproteins such as Ras or Bcr/Abl, which allowed for both factor-independent proliferation and differentiation, or Bcl-2, which permitted factor-independent survival but not proliferation. (nih.gov)
  • Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
  • Both C/EBPs command and control molecular programs of proliferation and differentiation into innate immune cells , including various types of myeloid cells. (mdc-berlin.de)
  • This includes the analysis of genes in germline stem cell proliferation and differentiation. (ncl.ac.uk)
  • Hematopoiesis, in particular the genetic control of hematopoietic cell proliferation and differentiation is also studied in human cell cultures and transgenic mouse model systems. (lu.se)
Hematopoiesis17
  • Lei said that in the next phase, the research team needs to conduct comprehensive detection and analysis of this group of hematopoietic stem cells, and screen out the relevant genes affecting the early hematopoiesis differentiation of human pluripotent stem cells in the space environment by comparing with the ground control group. (pdnews.cn)
  • Wnt signaling increases hematopoietic stem cell self-renewal and is activated in both myeloid and lymphoid malignancies, indicating involvement in both normal and malignant hematopoiesis. (ox.ac.uk)
  • Bone marrow-derived hematopoietic and mesenchymal stem cells as targets for Histoplasma capulatum infection: implications for hematopoiesis, differentiation, and proliferation. (urosario.edu.co)
  • To determine whether the interaction of hematopoietic stem cells (HSCs) and bone marrow-derived mesenchymal stem cells (BMMSCs) with Histoplasma capsulatum affects hematopoiesis, differentiation and proliferation of these precursor cells. (urosario.edu.co)
  • BMMSCs) with Histoplasma capsulatum affects hematopoiesis, differentiation and proliferation of these precursor cells. (urosario.edu.co)
  • Hematopoiesis is an important model for stem cell differentiation with great medical significance. (europa.eu)
  • Here, we propose to uncover the hierarchy and regulatory mechanisms controlling hematopoiesis by combining comprehensive single-cell RNA-Seq analyses, modelling approaches, advanced functional assays, single-cell CRISPR screens, knockout models and epigenetic profiling. (europa.eu)
  • We will pursue the following aims: (i) Generate a refined model of hematopoiesis by comprehensive single-cell RNA-Seq profiling of hematopoietic progenitors, (ii) validate the predicted model by in vivo functional developmental assays and then (iii) test candidate transcription and chromatin factors uncovered by our model for their role in controlling progression towards various lineages using single-cell measurements combined with CRISPR screens. (europa.eu)
  • Together, our study is expected to generate a revised and high-resolution hematopoietic model and decipher the regulatory networks that control hematopoiesis. (europa.eu)
  • We apply targeted mouse genetics in combination with experimental hematopoiesis, genetic engineering of hematopoietic cells, human iPSC hematopoietic differentiation, proteomics and biochemistry. (mdc-berlin.de)
  • Together with the associated research group of Dr. Alexander Mildner we perform ATACseq, ChIPseq and single cell analysis to disclose fundamental epigenetic mechanisms in hematopoiesis, homeostasis, autoimmune diseases, and in leukemogenesis. (mdc-berlin.de)
  • DNMT3A is a critical regulator of hematopoietic stem cell (HSC) differentiation and somatic DNMT3A mutations are frequent in hematologic malignancies and clonal hematopoiesis. (haematologica.org)
  • While responses to inflammation may be necessary to maintain normal and emergency hematopoiesis, it may be deleterious over the long term, with prolonged inflammation leading to stem cell exhaustion (Cell Reports, 2016). (bcm.edu)
  • Clonal hematopoietic stem cell disorders characterized by ineffective hematopoiesis and peripheral cytopenias. (standardofcare.com)
  • The Division of Molecular Hematology (DMH) aims to generate new knowledge about the regulation of blood cell development by applying recent advances in stem cell technology, in order to dissect the molecular regulation of hematopoiesis. (lu.se)
  • Myelodysplastic syndrome (MDS) in childhood encompasses a diverse group of bone marrow disorders that share a common clonal defect of stem cells and that result in ineffective hematopoiesis with dysplastic changes in the marrow. (medscape.com)
  • Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)
Bone marrow hematopoietic stem2
Embryonic stem11
Transplantation8
  • BACKGROUND: Autologous stem cell transplantation (autoSCT) can extend remission of mantle cell lymphoma (MCL), but the management of subsequent relapse is challenging. (koreamed.org)
  • BACKGROUND: This study aimed to assess the outcome of stem cell transplantation (SCT), including overall survival (OS), failure-free survival (FFS) and graft-versus-host disease (GvHD)-free/failure-free survival (GFFS), and to analyze prognostic. (koreamed.org)
  • The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
  • In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
  • Hematopoietic stem cell transplantation (HSCT) is a life-saving procedure for treatment of malignant and non-malignant disorders and is usually a last resort for those with no other treatment options available. (coriell.org)
  • MDS is rare in childhood and may have a rapidly progressive course with an extremely poor prognosis without hematopoietic stem cell transplantation (HSCT). (medscape.com)
  • 1 line of systemic therapy in case of participants ineligible for high-dose chemotherapy and autologous Hematopoietic stem cell transplantation (HSCT). (who.int)
  • The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)
Progenitors8
  • We examined hematopoietic stem cells at four stages of differentiation, lineage-committed progenitors, and mature myeloid and lymphoid cells. (nih.gov)
  • The cell lines were established by conditional immortalization of primary murine marrow progenitors with an estrogen-regulated E2a/Pbx1-estrogen receptor fusion protein. (nih.gov)
  • Loss of hematopoietic stem cell function was associated with decreased expression of Cdkn1a (encoding the cell cycle inhibitor p21(cdk)), Sfpi1, Hoxb4 and Bmi1 (encoding the transcription factors PU.1, HoxB4 and Bmi-1, respectively) and altered integrin expression in Lin(-)Sca-1(+)c-Kit(+) cells, whereas PU.1 was upregulated in erythroid progenitors. (ox.ac.uk)
  • Recently we have developed an experimental and analytical method, termed massively parallel single-cell RNA-Seq (MARS-Seq), for unbiased classification of individual cells from their native context and successfully applied it for characterization of immune and hematopoietic progenitors. (europa.eu)
  • Exciting preliminary result show that indeed our approach is starting to uncover the complexity of hematopoietic progenitors and the regulatory circuits driving hematopoietic decisions. (europa.eu)
  • Dysregulation of these master switches may affect differentiation, which can lead to "confused" cells as the potential progenitors of leukemia. (mdc-berlin.de)
  • However, very little is known about the mechanisms by which inflammatory cytokines affect the hematopoietic progenitors that reside in bone marrow. (bcm.edu)
  • Indeed, one critical question contemplated at the Workshop was whether tumors derive from organ stem cells that retain self-renewal properties but acquire epigenetic and genetic changes required for tumorigenicity or whether tumor stem cells are proliferative progenitors that acquire self-renewal capacity. (aacrjournals.org)
Lineages7
  • Hematopoietic stem cells (HSC) give rise to all types of blood lineages, including red blood cells (RBC). (lu.se)
  • The variation among induced pluripotent stem cells (iPSCs) in their differentiation capacity to specific lineages is frequently attributed to somatic memory. (nih.gov)
  • The differentiation of mesenchymal stem cells (MSCs) into multiple cell lineages can be exploited as an attractive strategy for cell-based therapy and regenerative medicine [ 1 ]. (hindawi.com)
  • In addition to using renewable sources of pluripotent stem cell-derived human hematopoietic lineages for therapeutic and investigative applications, she is genome editing pluripotent stem cells using the CRISPR-CAS9 technology. (chop.edu)
  • The blood cell counts in TERT-depleted zebrafish embryos are markedly decreased and hematopoietic cell differentiation is impaired, whereas other somatic lineages remain morphologically unaffected. (harvard.edu)
  • The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
  • HSCs are able to self-renew and differentiate into all blood cell lineages. (coriell.org)
Somatic6
  • The telomerase-expressing somatic cells observed in this study are not thought to be immortal, and expression was not correlated with cell cycle distribution or differentiation state. (nih.gov)
  • All GS-2 iPSC clones displayed a normal karyotype (46XX or 46XY) and were shown to express the same RAB27A gene mutation that was present in the original somatic donor cells. (biomedcentral.com)
  • Somatic DNMT3A gene mutations are also found relatively frequently in another form of blood cancer called T-cell acute lymphoblastic leukemia. (medlineplus.gov)
  • These data demonstrated that variations in the hematopoietic differentiation capacity of iPSCs are not attributable to somatic memories of their origins. (nih.gov)
  • We research human embryonic stem (ES) cells, germline stem cells and somatic stem cells. (ncl.ac.uk)
  • During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Self-renewal and differentiation3
  • My laboratory studies signal transduction pathways regulate hematopoietic stem cell (HSC) self-renewal and differentiation as well as drug resistance of hematological malignancies. (coriell.org)
  • A better understanding of the complex signaling network regulating the balance between HSC self-renewal and differentiation will allow us to manipulate HSCs to enhance their therapeutic efficacy. (coriell.org)
  • A broader interest of our laboratory is to understand the molecular regulation of HSCs both from the perspectives of self-renewal and differentiation, and how these features become altered upon disease-causing or disease-associated perturbations. (lu.se)
Precursors5
  • The TAL1/SCL transcription factor regulates cell cycle progression and proliferation in differentiating murine bone marrow monocyte precursors. (nih.gov)
  • Differentiation of induced pluripotent stem cells (iPSCs) into stromal cells and hematopoietic precursors. (nih.gov)
  • The analysis revealed that hematopoietic commitment capacity (PSCs to hematopoietic precursors) is correlated with the expression level of the IGF2 gene independent of the iPSC origins. (nih.gov)
  • Hematopoietic stem cells (HSCs) are the precursors of all cells of the peripheral blood, and they usually reside in a quiescent state in the bone marrow. (bcm.edu)
  • The paradox is that the mutation provides advantage at the stem cell level and progenitor cells, with a disadvantage at the level of hematopoietic precursors. (standardofcare.com)
IPSC4
  • Induced pluripotent stem cells (iPSC) express genes associated with pluripotency, have the capacity for infinite expansion, and can differentiate into cells from all three germ layers. (biomedcentral.com)
  • Especially in cases where sufficient cells cannot be obtained due to the rarity of the disorder, iPSC models have shown great potential in providing research material to develop new treatments or diagnostics, but also to study the pathophysiology of the diseases [ 10 , 12 ]. (biomedcentral.com)
  • Studies employ a transgenic animal model of spondyloarthritis and cells from human subjects including induced pluripotent stem cells (iPSC), to evaluate effects of HLA-B27 expression and misfolding on ER stress and its consequences for cytokine production and cellular function. (nih.gov)
  • In this study, we compared hematopoietic differentiation capacity of 35 human iPSC lines derived from four different tissues and four embryonic stem cell lines. (nih.gov)
Mesenchymal5
  • Mesenchymal stem cell medium was used in the control groups. (scielo.br)
  • Mesenchymal stem cells (MSCs) possess great therapeutic potential. (hindawi.com)
  • The effects of erythropoietin (EPO) on the behaviors of bone marrow mesenchymal stem cells (BMSCs) subjected to mechanical stretch remain unclear. (hindawi.com)
  • Notably, it promotes the osteogenic differentiation of bone mesenchymal stem cells (BMSCs) [ 15 - 17 ]. (hindawi.com)
  • Conclusions: The cells isolated from dental pulp exhibited characteristics compatible with those expected for mesenchymal stem cells (MSCs) and are good candidates for cell therapy applications and tissue bioengineering. (bvsalud.org)
Myeloid8
  • The molecular pathways of normal myeloid differentiation, as well as the mechanisms by which oncogenes disrupt this process, remain poorly understood. (nih.gov)
  • This report describes clonal, granulocyte-macrophage colony-stimulating factor-dependent myeloid cell lines that exhibit these properties. (nih.gov)
  • These myeloid cell lines provide tools for examining the biochemical and genetic pathways that accompany normal differentiation as well as a system in which to dissect how other leukemic oncoproteins interfere with these pathways. (nih.gov)
  • Researchers speculate that the altered gene activity prevents hematopoietic stem cells from differentiating normally, which leads to the overproduction of abnormal, immature white blood cells characteristic of acute myeloid leukemia. (medlineplus.gov)
  • As in cytogenetically normal acute myeloid leukemia (described above), the mutations disrupt the normal pattern of methylation in cells, which blocks differentiation. (medlineplus.gov)
  • We discovered that interferon gamma (IFNγ) promotes hematopoietic stem cell (HSC) division and myeloid differentiation in a mouse model of Mycobacterium avium infection (Nature 2010). (bcm.edu)
  • Bone marrow cells didn't develop into myeloid and red blood cells. (embl.org)
  • A heterogenous group of myeloid disorders characterized by somatically mutated hematopoietic stem cells,the presence of variable peripheral cytopenias, and a broad risk of progression to acute myeloid leukemia (AML). (standardofcare.com)
Regulator of hematopoietic1
  • In response to the inhibition of rRNA transcription, nucleolar protein MYBBP1A was translocated from the nucleolus to the nucleoplasm and interacted with c-Myc, which is one of the master regulator of hematopoietic differentiation. (nii.ac.jp)
Regulates1
  • Stromal cell CD9 regulates differentiation of hematopoietic stem/progenitor cells. (bdbiosciences.com)
Characterization3
  • Heterogeneity within hematopoietic progenitor populations has considerably limited characterization and molecular understanding of lineage commitment in both health and disease. (europa.eu)
  • First steps in this direction are the characterization of relevant immune cells, optimization of culture conditions and in vitro differentiation from precursor to mature immune cells. (uni-potsdam.de)
  • The pluripotent stem cell technology represents a major breakthrough for studying disease because these cells express human genes of interest, they can be differentiated in vitro to the three germ layers and derivative tissues, they can be genetically manipulated, and they allow for easy access to cells for in-depth structural, functional, and molecular characterization. (chop.edu)
Tissues8
  • It was found that expression of NR2F6 was consistently upregulated in neoplastic tissues in leukemic, ovarian cancer and endometrial cancer as compared to non-malignant tissues, while the silencing of NR2F6 induces a reduction of cancer cell proliferation, inhibiting clonogenicity and self-renewal of proliferating cancer cells, and induces differentiation. (wikipedia.org)
  • Fig. 3: Single-cell transcriptomics of dorsal and ventral MiSTR tissues. (nature.com)
  • Although different stem cells, morphogens and scaffolds can be combined to simulate natural odontogenic regeneration, it is crucial to determine which options most closely replicate the characteristics of human dental tissues. (scielo.br)
  • Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. (rndsystems.com)
  • hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
  • However, MSCs need to be expanded in vitro in order to obtain sufficient cells for clinical trials since they are extremely rare in various tissues. (hindawi.com)
  • The ability to generate isogenic lines has eliminated the clonal heterogeneity observed when comparing cell lines of different genetic backgrounds during differentiations to derivative tissues and downstream functional applications. (chop.edu)
  • Stem cells are cells with a low degree of differentiation that have the capacity to reproduce and can generate differentiated cells of several different types of tissues 1 . (bvsalud.org)
Vitro differentiation2
  • erythroid cells after in vitro differentiation, despite having similar frequencies of phenotypic HSC initially. (lu.se)
  • In vitro differentiation of GS-2 iPSCs into hematopoietic stem and progenitor cells was done using Op9 feeder layers and specified media. (biomedcentral.com)
Maturation2
Differentiate7
  • This stem cell will further mature and differentiate to form a group of hematopoietic stem cells resembling a cluster of grapes. (pdnews.cn)
  • GS-2 iPSCs showed the capacity to differentiate into cells of the hematopoietic lineage. (biomedcentral.com)
  • Hematopoietic stem cells in the bone marrow sustain their own maintenance and give rise to "transit amplifying", lineage committed cells that terminally differentiate into distinct, mostly short-lived cell types. (mdc-berlin.de)
  • Stem cells in the body are responsible for the regeneration of tissue in the body and can differentiate to form an array of specialized cells. (medicalnewstoday.com)
  • Specifically, the division of a stem cell can result in the formation of identical daughter stem cells and/or cells that can differentiate to assume different fates. (medicalnewstoday.com)
  • Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
  • These cancer stem cells have the capacity to both divide and expand the cancer stem cell pool and to differentiate into the heterogeneous nontumorigenic cancer cell types that in most cases appear to constitute the bulk of the cancer cells within the tumor. (aacrjournals.org)
20201
  • We recently demonstrated that the cell surface expressed protein BST2 is a critical mediator of IFNγ-dependent HSC activation, and deletion of BST2 can help preserve HSCs in the setting of chronic infection (Cell Reports, 2020). (bcm.edu)
HSCs6
  • Although HSCs are the most well-characterized tissue-specific stem cells, our understanding of the molecular pathways that control HSC maintenance and function is still limited. (coriell.org)
  • Our research focuses on how hematopoietic stem cells (HSCs) and their immediate offspring are regulated in situations such as infections, stress, aging and leukemia. (lu.se)
  • We have developed and applied FACS-based high-resolution cell fractionation procedures to map the differentiation processes from HSCs. (lu.se)
  • Our goal is to understand, at cellular and molecular levels, what controls HSCs and the fate of other immature blood cells. (lu.se)
  • It is believed that benzene and its metabolites target hematopoietic stem cells (HSCs) to cause toxicity and cancer in the hematopoietic system. (cdc.gov)
  • HQ reduced the proliferation and the differentiation and colony formation, but increased the apoptosis of both YS-HSCs and BM-HSCs. (cdc.gov)
Neuronal2
  • 2001. Subchronic dermal application of N,N-diethyl m-toluamide (DEET) and permethrin to adult rats, alone or in combination, causes diffuse neuronal cell death and cytoskeletal abnormalities in the cerebral cortex and the hippocampus, and purkinje neuron loss in the cerebellum. (cdc.gov)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
Progenitor cell1
  • By studying murine transgenic leukemia models developed in our laboratory, we are currently exploring the relationships between hematopoietic progenitor cell identities and the development and progression of acute leukemia. (lu.se)
Pluripotent stem2
  • Bisulfite converted genomic DNA lysates from induced pluripotent stem cell and embryonic stem cell lines were hybridized to Illumina HumanMethylation450 BeadChip. (nih.gov)
  • Dr. French came to CHOP in 2008 to establish the Human Pluripotent Stem Cell Core in the Center for Cellular and Molecular Therapeutics. (chop.edu)
Malignant hematopoietic2
  • There is clonal proliferation of malignant hematopoietic stem cells, dysregulated cellular differentiation, and compromised tissue function. (standardofcare.com)
  • Melinda is highly proficient in the use of in vitro and in vivo assays for normal and malignant hematopoietic stem and progenitor cells, determination of self-renewal, expansion and lineage specific differentiation capabilities, and the use of induced pluripotent stem cells for the investigation of hematopoietic disorders and leukemic progression and the testing of targeted therapeutics. (edu.au)
IPSCs1
  • Regenerative therapy holds great promise in the development of cures of some untreatable diseases such as cardiovascular diseases, and pluripotent stem cells (PSCs) including induced PSCs (iPSCs) are the most. (koreamed.org)
HSCT1
  • The overall death infections that occurred in 2 clusters of hematopoietic stem rate was 50%, and death occurred within 8 days after diag- cell transplant (HSCT) recipients in our institution prompt- nostic culture collection. (cdc.gov)
Clonal3
  • Criteria for diagnosis of MDS consist of anemia, thrombocytopenia, or neutropenia that persist for six months or longer, dysplasia greater than 10% in at least one bone marrow cell lineage, and MDS associated clonal cytogenetic abnormalities or molecular markers. (standardofcare.com)
  • The mutation occurs in hematopoetic stem cells capable of self renewal and additional mutations are associated with clonal progression may occur in progenitor cells conferring a self renewal capability. (standardofcare.com)
  • The clonal outgrowth of mutant stem cells favors and abnormal hematopoietic stem cell niche. (standardofcare.com)
Antigens2
Molecular5
  • Consequently, investigation of molecular heterogeneity in the differentiation potential of HSPC is vital to identify novel regulators that affect generation of specific cell types, especially RBC. (lu.se)
  • We study the development of blood cells and the emergence of leukemia by focusing on the molecular genetics of self-renewal and its connection to differentiation into functional cells of the innate immune system, like macrophages, granulocytes, and dendritic cells. (mdc-berlin.de)
  • Over the past decades, molecular biologists have identified several pathways - sequences of molecules which manage the flow of information within the cell - responsible for major biological processes. (embl.org)
  • Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
  • Little is known about molecular mecha- desmosomal glycoproteins, resulting in the nisms affecting mast cell and T lympho- loss of keratinocyte cell-cell adhesion [ 1 ]. (who.int)
Genes3
  • In addition, their osteogenic differentiation potential was increased, and genes involved in cell adhesion, FGF-2 signalling, cell cycle, stemness, cell differentiation, and cell proliferation were upregulated, compared to that of the MSCs cultured on uncoated plates. (hindawi.com)
  • Scientists have long had the tools to identify the genes being transcribed in a single cell. (acs.org)
  • In some cases, "master" genes have been described which control differentiation versus self-renewal. (justia.com)
Dental pulp stem cells6
  • This study was designed to determine the in vivo performance of three different materials as scaffolds for dental pulp stem cells (DPSC) undergoing induced odontogenic differentiation. (scielo.br)
  • Dental pulp stem cells (DPSC) seem to be the most appropriate cell group for the regenerative approach. (scielo.br)
  • Dental pulp stem cells: state of the art and suggestions for a true translation of research into therapy. (scielo.br)
  • Odontogenic capability: bone marrow stromal stem cells versus dental pulp stem cells. (scielo.br)
  • The odontogenic differentiation of human dental pulp stem cells on nanofibrous poly(L-lactic acid) scaffolds in vitro and in vivo. (scielo.br)
  • OBJECTIVES: This study investigated the indirect effect of calcium-enriched mixture (CEM) cement and mineral trioxide aggregate (MTA), as 2 calcium silicate-based hydraulic cements, on human dental pulp stem cells (hDPSCs). (koreamed.org)
Leukemia7
  • The CD9 antibody, clone M-L13, is derived from the hybridization of mouse myeloma cells with spleen cells isolated from mice immunized with common acute lymphoblastic leukemia (cALL) cells. (bdbiosciences.com)
  • Experimental hematology, leukemia research, in combination with stem cell research strives to untangle the underlying "grammar" of transcription factor networks. (mdc-berlin.de)
  • I found that rRNA transcription was downregulated during hematopoietic differentiation in both human leukemia cell line and mouse hematopoietic stem cells. (nii.ac.jp)
  • Dr. French and her colleagues have shown a number of hematopoietic pathologies to be successfully recapitulated through differentiation of patient-derived induced pluripotent stem cells, including Glanzmann thrombasthenia, Down syndrome, and juvenile myelomonocytic leukemia. (chop.edu)
  • Because defects in such pathways and in the development of stem cells frequently lead to leukemia and other diseases, the work should give researchers a new handle on processes within cells that lead to cancer. (embl.org)
  • Overexpression of FLI1 in erythroblasts causes inhibition of differentiation and ultimately the development of pre-T cell lymphoblastic leukemia/lymphoma [ 11 ]. (biomedcentral.com)
  • Aberrant regulation of blood cell formation underlies a wide range of hematological disorders, including leukemia. (lu.se)
Cellular7
  • Cellular senescence, which is morphologically characterized by an enlarged and flattened cell shape, was first described by Hayflick [ 9 ]. (hindawi.com)
  • Cellular senescence refers to active cells that eventually enter a state of irreversible growth arrest. (hindawi.com)
  • In this study, I have tried to address the role of the nucleolus in cellular differentiation. (nii.ac.jp)
  • These results suggest that the downregulation of rRNA transcription accelerates cellular differentiation at least in hematopoietic cells. (nii.ac.jp)
  • Analysis of the cellular phenotype showed that the cells were negative for CD45, CD69, CD117 and HLADR, and positive for CD13, CD44, CD73, CD90 and CD105. (bvsalud.org)
  • However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
  • ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)
Vivo4
  • In vivo differentiation to cells from all three germlines was tested using a teratoma assay. (biomedcentral.com)
  • The use of such cell lines allows the ex vivo induction of an immune response and makes it independent from experimental animals. (uni-potsdam.de)
  • The participants critically discussed the need for a precise definition of cancer stem cells, the requirement for new markers and more rapid and tractable in vitro and in vivo assays, and the need to develop drug screening strategies to selectively target cancer stem cells to generate therapeutics for this subpopulation of cells that could be resistant to classic treatments while possessing potent tumor-forming capacity. (aacrjournals.org)
  • Pulp stem cells (PSCs) are able to form ectopic dentin in vitro and in vivo and also to generate a dentine-pulp complex composed of a mineralized matrix, with dentinal tubules that are aligned and filled with odontoblastic prolongations, containing vascularized pulp tissue, in a similar arrangement to that observed in natural dental structures 3,6 . (bvsalud.org)
Disorders5
  • She is continuing this work using human pluripotent stem cells to study pediatric and adult genetic diseases, including hematological disorders. (chop.edu)
  • Our recent work identified a new connection between GSK3 and PPAR-δ/mitophagy pathway, which will provide new insights into the mechanisms of the regulation of HSC activity and may improve the therapy of many hematopoietic disorders. (coriell.org)
  • This will provide key insights on how ubiquitin signaling plays a vital role in stem cell differentiation and how perturbations in these networks can lead to disorders such as cancer. (lu.se)
  • Pemphigus vulgaris (PV) is a life-threate- disorders and to shed a light on the role of ning blistering skin disease in which pa- mast cells in autoimmune diseases [ 7 ]. (who.int)
  • PC-ALCL is one of the primary cutaneous CD30 + T-cell lymphoproliferative disorders, a wide spectrum of disease, with lymphomatoid papulosis (LyP) at the benign end of the spectrum and PC-ALCL at the malignant end. (medscape.com)
Tumor5
  • A workshop was convened by the AACR to discuss the rapidly emerging cancer stem cell model for tumor development and progression. (aacrjournals.org)
  • Either mechanism is different from the widely held notion that most cells in a tumor should be competent for tumor formation. (aacrjournals.org)
  • In the cancer stem cell model of tumors, there is a small subset of cancer cells, the cancer stem cells, which constitute a reservoir of self-sustaining cells with the exclusive ability to self-renew and maintain the tumor. (aacrjournals.org)
  • If cancer stem cells are relatively refractory to therapies that have been developed to eradicate the rapidly dividing cells within the tumor that constitute the majority of the nonstem cell component of tumors, then they are unlikely to be curative and relapses would be expected. (aacrjournals.org)
  • ALCL was recognized in 1985, when tumor cells consistently demonstrated labeling by the monoclonal antibody Ki-1, a marker later shown to recognize the CD30 antigen. (medscape.com)
Immature3
Gene expression1
  • Differentiation was normal and terminal as evidenced by morphology, cell surface markers, gene expression, and functional assays. (nih.gov)
Immune9
  • It was found that antigen receptor stimulation-induced NF-AT/AP-1 activity is regulated through the nuclear receptor NR2F6 (NR2F6 acts as a direct repressor of NF-AT/AP-1 transactivation) and that by preventing NR2F6 function, transcriptional activation of NF-AT/AP-1 is enhanced in immune cells which leads to an augmented immune response. (wikipedia.org)
  • We assayed telomerase activity in single cells of the hematopoietic and immune systems. (nih.gov)
  • Griscelli syndrome type 2 (GS-2) is a rare, autosomal recessive immune deficiency syndrome caused by a mutation in the RAB27A gene, which results in the absence of a protein involved in vesicle trafficking and consequent loss of function of in particular cytotoxic T and NK cells. (biomedcentral.com)
  • In our current project we are working on the generation of immortal immune cell lines in order to improve our In vitro Immunization approach. (uni-potsdam.de)
  • Thereby the in vitro Immunization represents an alternative to the conventional hybridoma technique because cell lines would provide permanent and limitless numbers of the immune cells needed and could be generated from different host species. (uni-potsdam.de)
  • As the immune cells of the central nervous system (CNS), microglia could influence neurogenesis through the M1 and M2 subtypes, and these may promote depressive-like behaviors. (frontiersin.org)
  • Microglial cells are the brain's immune cells in the CNS. (frontiersin.org)
  • Furthermore, this work may elucidate how inflammatory signals affect the pattern of HSC differentiation and, in turn, downstream immune responses, as well as how infections can contribute to aging and oncogenesis. (bcm.edu)
  • The production of distinct blood cell types can be selectively adapted to meet an individual's acute needs, such as for instance the rapid and enhanced generation of innate immune cells following infection. (lu.se)
Inhibition of differentiation1
  • So, when inhibition of differentiation of stem cell is desired, inhibition of differentiation is achieved through induction of increased NR2F6 activity. (wikipedia.org)
Regeneration6
  • In recent years, foreign scientists have also reported several times on research regarding stem cell growth and tissue regeneration carried out during space flight missions, such as blood stem cells for astronauts with anemia. (pdnews.cn)
  • This can provide more beneficial assistance for the future use of stem cell regeneration to serve human health. (pdnews.cn)
  • Consequently, PLDL and PDL seemed to be promising scaffold candidates for odontogenic regeneration at least as HA-TCP, when they were applied with the DPSC induced for odontogenic differentiation. (scielo.br)
  • These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
  • Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
  • Our study suggests that melanocyte stem cells are mobile but can start the regeneration of hair melanocytes only when they are present in a specific area within the hair follicle (hair germ compartment). (medicalnewstoday.com)
Osteogenic7
  • This study was therefore aimed at establishing the dose-response effect of EPO stimulation on rat BMSCs and investigating the effects of mechanical stretch combined with EPO on the proliferation and osteogenic differentiation of BMSCs. (hindawi.com)
  • The proliferation and osteogenic differentiation of rat BMSCs were examined and compared using EPO with different concentrations. (hindawi.com)
  • The osteogenic differentiation of BMSCs was detected by alkaline phosphatase (ALP) staining, and the mRNA expression levels of ALP, OCN, COL, and Runx2 were detected by qPCR. (hindawi.com)
  • Mechanical stretch combined with 20 IU/ml EPO promoted proliferation and osteogenic differentiation of BMSCs. (hindawi.com)
  • EPO was able to promote the proliferation and osteogenic differentiation of BMSCs, and these effects were enhanced when combined with mechanical stretch. (hindawi.com)
  • This study was therefore aimed at addressing three key issues: First, is there a dose-dependent effect of EPO on the proliferation and osteogenic differentiation of BMSCs? (hindawi.com)
  • The objective of this study is to isolate and characterize stem cells from the pulp of permanent third molars and analyze the cells obtained in terms of their morphology and chondrogenic, osteogenic and adipogenic cell differentiation. (bvsalud.org)
Biology3
  • Measurements of cell mixtures can hide differences between cells that might be important in biology or disease studies. (acs.org)
  • Researchers are putting single-cell proteomics to work in fields like developmental biology, cell biology, cancer biology, and neuroscience. (acs.org)
  • I am currently a Senior Scientist for Stem Cell Biology and Gene Engineering at the Coriell Institute for Medical Research. (coriell.org)
Induce5
  • An odontogenic medium modified by the addition of recombinant human bone morphogenetic protein 2 was used in the experimental groups to induce differentiation. (scielo.br)
  • During erythropoiesis, the cells start to produce hemoglobin, lose nuclei and organelles, and induce plasma membrane remodeling. (elifesciences.org)
  • Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
  • culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
  • Furthermore, mechanical stretch may induce the differentiation of BMSCs into mature osteoblasts and enhance the deposition of the bone matrix [ 18 - 20 ]. (hindawi.com)
Therapeutic4
  • Stem cell research arose from the need to explore new therapeutic possibilities for intractable and lethal diseases. (koreamed.org)
  • She is an internationally recognized researcher involved in multi-investigator teams that utilize pluripotent stem cells for modeling human disease to study mechanism, development, and establish new therapeutic modalities. (chop.edu)
  • The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
  • The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
Methods8
  • Our methods and models may provide a platform for future medical advancements including a large-scale European collaborative project to discover a comprehensive human hematopoietic tree. (europa.eu)
  • Now single-cell proteomics is possible too, thanks to new methods in sample processing and analysis. (acs.org)
  • People have been able to detect a handful of proteins in individual cells using fluorescence- or antibody-based methods. (acs.org)
  • The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
  • The invention also includes cells, cell lines, testing models and culture systems used in the methods of the present invention and differentiated cells produced therefrom. (justia.com)
  • Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
  • Methods: Pulp tissue was collected from permanent third molars and digested and then the cells were seeded onto plates containing HDMEM medium. (bvsalud.org)
  • CD117 by immunohistochemical methods sion molecules, involved in cell-cell and in order to clarify the role of the infiltrating cell matrix interactions and thought to take inflammatory cells in the pathomechanisms part in cell motility [ 2,3 ]. (who.int)
Phenotypes2
  • The presence of neurotransmitter receptors in microglia illustrates their functional connection to neurons and this receptor activation could cause microglial cells to perform different activation phenotypes ( Pocock and Kettenmann, 2007 ). (frontiersin.org)
  • Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
Erythroid1
  • Orthologous to human TAL1 (TAL bHLH transcription factor 1, erythroid differentiation factor). (nih.gov)
Proteins10
  • The differentiation of the cells could be arrested by heterologous oncoproteins including AML1/ETO, PML/RARalpha, PLZF/RARalpha, Nup98/HoxA9, and other Hox proteins. (nih.gov)
  • Bone morphogenetic proteins (BMP), known as active components of odontogenic differentiation and tooth development, have been used as a growth factor in an appropriate medium. (scielo.br)
  • Key gene regulatory proteins control cell multiplication and instruct cells to adopt distinct cell fates. (mdc-berlin.de)
  • Hematopoietic transcription factors (= gene regulatory proteins) concertedly control cell proliferation, lineage commitment, and cell differentiation. (mdc-berlin.de)
  • Individual cells' proteins vary. (acs.org)
  • Researchers have long had tools to look at nucleic acids at the single-cell level, but studying single cells' proteins hasn't been as easy. (acs.org)
  • Now, the goal is a full readout of all the proteins being made in a cell. (acs.org)
  • Induced p53 deficiency, as well as overexpression of the anti-apoptotic proteins Bcl-2 and E1B-19K, significantly relieves the decreased blood cells numbers caused by zTERT knockdown, but not the impaired blood cell differentiation. (harvard.edu)
  • Currently we are identifying crucial proteins in blood forming stem cells that are regulated by ubiquitin signaling. (lu.se)
  • Since each ubiquitin ligase can recognize its target proteins through a specific degron, we are interested in delineating ubiquitin ligase-target protein interactions that are triggered by differentiation stimulus in stem cells. (lu.se)
CRISPR2
  • Highly Efficient CRISPR-Cas9-Mediated Genome Editing in Human Pluripotent Stem Cells. (chop.edu)
  • Using a CRISPR Cas9-guided immunoprecipitation assay, we identify a circular RNA in the FLI1 promoter chromatin complex, consisting of FLI1 exons 4-2-3, referred to as FECR1.Overexpression of FECR1 enhances invasiveness of MDA-MB231 breast cancer cells. (biomedcentral.com)
Stromal cells1
Mutations2
  • DNMT3A gene mutations that cause DNMT3A overgrowth syndrome are found in all of the body's cells and lead to a decrease in normal enzyme function. (medlineplus.gov)
  • they are typically found in a small percentage of cells and the mutations are not inherited. (medlineplus.gov)
Cytokines2
  • A major limitation in approaching this problem has been the lack of suitable cell lines that exhibit normal, terminal, and synchronous differentiation in the absence of endogenous oncoproteins and in response to physiologic cytokines, and whose differentiation can be arrested by ectopically expressed human oncoproteins. (nih.gov)
  • These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
Erythropoiesis1
  • Mechanistically, we identified dysregulated erythropoiesis with reduced numbers of reticulocytes, the preferred host cells of P. yoelii , in Ac-deficient mice. (elifesciences.org)