AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation Science
RNACell DifferentiationNucleic Acid HybridizationRNA TransportRNA, MessengerRNA-Binding ProteinsOogenesisMolecular Sequence DataDrosophila ProteinsIn Situ HybridizationBase SequenceCells, CulturedGene Expression Regulation, Developmental3' Untranslated RegionsGene Expression RegulationTranscription FactorsDrosophilaCell LineOocytesCell NucleusCell PolarityAmino Acid SequenceCytoplasmSignal TransductionProtein BiosynthesisTranscription, GeneticReverse Transcriptase Polymerase Chain ReactionImmunohistochemistryHomeodomain ProteinsMutationStem CellsDrosophila melanogasterDNA-Binding ProteinsMyosin Type VTrans-ActivatorsRibonucleoproteinsCell DivisionEmbryonic Stem CellsRepressor ProteinsGreen Fluorescent ProteinsNuclear ProteinsEmbryo, NonmammalianCell LineageTransfectionProtein BindingMicroscopy, FluorescencePhenotypeBlotting, WesternNuclear Localization SignalsActinsTransforming Growth Factor alphaInsect ProteinsDNA PrimersCell ProliferationCloning, MolecularTretinoinProtein TransportEgg ProteinsRecombinant Fusion ProteinsBody PatterningAnimals, Genetically ModifiedNeuronsMice, Inbred C57BLMice, KnockoutCell CompartmentationCytoskeletonGene ExpressionBiological TransportBlotting, NorthernMice, TransgenicLuminescent ProteinsActive Transport, Cell NucleusRNA StabilityGene Expression ProfilingChick EmbryoMembrane ProteinsMicrotubulesProteinsProtein IsoformsFluorescent Antibody TechniqueNucleic Acid ConformationTumor Cells, CulturedSubcellular FractionsSex DifferentiationOvaryRNA, Small InterferingGerm CellsTransgenesGenes, ReporterHeterogeneous-Nuclear RibonucleoproteinsModels, BiologicalSaccharomyces cerevisiae ProteinsPromoter Regions, GeneticGenes, InsectTime FactorsDown-RegulationSaccharomyces cerevisiaeUp-RegulationRNA, FungalPolyribosomes
ProteinsGene expressionGenesApoptosisSingle-cell RNA-seqSubcellular localizationProliferationEmbryonicCytoplasmicLncRNAsSequencesMaturationMiRNANeuronsTissuesMethylationVitroMoleculesEukaryoticPost-transcriptionalNoncoding RNASignaling pathwayGonadal differentiationTranscriptionalMicroRNAsCellularInteractionsNeuronalSubsequentlyNucleotideFunctionalTranscriptomeBiologyEndogenousSitu hybridizationExonsNascentEpithelial cellSubsequentProcessesDendriticRegulationVariantsInteractionBiolSquamous cell carcHighly conservedMechanismsDegradationTumorReceptorsProgressionMolecularEvolutionarilyRevealStem cellsViral genomesDistalBiologicalExtracellularRegulatoryRepressionGeneticPolyadenylationExpressionMiRNAsAntibodiesNuclear
Proteins30
  • Of the ten mRNAs produced, six of them are translated into viable proteins. (wikipedia.org)
  • Heterogeneous population of RNA granules serve as motile units to translocate, store, translate, and degrade mRNAs in the dendrites contain cis -elements and trans -acting factors such as RNA-binding proteins and microRNAs to convey stimulus-, transcript-specific local translation. (frontiersin.org)
  • Through this interaction, mRNAs and their associative proteins form messenger ribonucleoprotein particles (mRNPs) that are actively transported along the cytoskeleton to intracellular destinations. (frontiersin.org)
  • We focus our research on the post-transcriptional regulation of embryonic myogenesis and adult muscle differentiation by RNA-binding proteins.We use zebrafish as a model, which is most suitable for in vivo and in vitro analyses using cell and molecular biology, genetics, and living imaging approaches. (sorbonne-universite.fr)
  • The post-transcriptional regulation of gene expression orchestrated by RNA-binding proteins is involved in a wide variety of physiological and pathological processes. (sorbonne-universite.fr)
  • In particular, we focus our research on the role of RNA-binding proteins in the post-transcriptional regulation of muscle differentiation and regeneration. (sorbonne-universite.fr)
  • RNA-binding proteins (RBPs) control RNA metabolism at multiple levels, and are critical for maintaining the homeostasis of protein synthesis during early development and in adult life. (sorbonne-universite.fr)
  • They allow cells to configure protein networks locally and exclude proteins from locations where their activity is harmful. (europa.eu)
  • RNA binding proteins recognize these sequences and assemble with their mRNA target into mRNPs. (europa.eu)
  • It is still not known which RBPs or adaptor proteins are essential to recruit microtubule-binding motor proteins to mRNPs and to which extend this varies between different transported mRNAs. (europa.eu)
  • Mutations affecting zipcodes, RBPs or motor-proteins required for neuronal mRNA localization were shown to lead to severe neurodegenerative diseases as ALS, FXTAS and FXS (7), underlining the need to understand the mechanisms that drive neuronal mRNA transport. (europa.eu)
  • Also in this specific case, it is not understood how RBPs, potential adaptors and motor proteins, which are essential for the transport of CaMKIIa-mRNA are mechanistically contributing to its correct localisation. (europa.eu)
  • Analyses of the molecular pathophysiology using the primary cells from the Tmem53 -/- mice and the TMEM53 knock-out cell lines indicates that TMEM53 inhibits BMP signaling in osteoblast lineage cells by blocking cytoplasm-nucleus translocation of BMP2-activated Smad proteins. (nature.com)
  • This is the first study describing the production and distribution of ETS-1 and ETS-2 mRNAs and proteins using in situ hybridization and immunohistochemistry in murine ocular tissue sections of normal control eyes and tumoral eyes from mice of the same age. (molvis.org)
  • S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (cancerindex.org)
  • Cell-type-specific interacting proteins collaborate to regulate the timing of Cyclin B protein expression in male meiotic prophase. (stanford.edu)
  • The different building blocks can affect the complementarity of the RNA molecules, alter their structure, and enable the binding of specific proteins that mediate various biochemical and cellular outcomes. (pharmaceuticalintelligence.com)
  • This enabled the diversified complementarity and secondary structures that allow RNA species to specifically interact with other components of the cellular machinery such as DNA and proteins. (pharmaceuticalintelligence.com)
  • The telomeres consist of repeating sequences and a set of special proteins, which interact with these repeats and spatially organize them in a specific manner, resulting in the formation of the nucleoprotein complex known as telomeric heterochromatin [ 6 , 7 ]. (actanaturae.ru)
  • Deficiency in ClpP induces an overload of mitochondrial misfolded/unfolded proteins, suppresses mitochondrial respiratory activity, increases mitochondrial oxidative damage and causes cell death. (springer.com)
  • Proteins that act both at the cell surface and intracellularly will be discussed in this thesis, with particular emphasis on the fibroblast growth factors. (beauty104.com.tw)
  • The FGFs are heparin -binding proteins and interactions with cell-surface associated heparan sulfate proteoglycans has been shown to be essential for FGF signal transduction. (beauty104.com.tw)
  • These proteins were found to be identical to the acidic and basic FGFs described by Gospodarowicz and coworkers. (beauty104.com.tw)
  • Although Rab11a subcellular localization has been demonstrated by transgenic zebrafish [10], and molecular function of Rab11 proteins in primary cilia membrane assembly in Kupffer Vesicle has been studied [6], the developmental expression profile of Rab11 proteins in zebrafish remains elusive. (researchsquare.com)
  • The richness of gene expression programs-in a given cell across time, across distinct cell types, and in response to diverse stimuli-results from the combinatorial logic of spatially organized nucleic acid elements that bind transcription factors, RNA binding proteins, and microRNAs. (columbia.edu)
  • One methodology for cancer biomarker discovery exploits the fact that glycoproteins produced by cancer cells have altered glycan structures, although the proteins themselves are common, ubiquitous, abundant, and familiar. (gene-quantification.de)
  • and (c) an isotope-coded glycosylation site-specific tagging high-throughput method to identify carrier proteins with the specific lectin epitope. (gene-quantification.de)
  • DDX5 is required for cell proliferation by controlling the transcription of genes expressing DNA replication proteins in cancer cells in which the DDX5 locus is amplified, and this has uncovered a dependence on DDX5 for cell proliferation. (aacrjournals.org)
  • To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
  • Myelinating Schwann cells form a myelin sheath around a single axon and express high levels of myelin-related proteins and messenger RNA (mRNA). (medscape.com)
Gene expression22
  • Its inactivation revealed its role directing alternations in cell division timing, growth polarity, as well as cell-specific gene expression, ultimately affecting organogenesis and cell differentiation. (wikipedia.org)
  • This process is mediated by 2 mutually exclusive programs of gene expression: 1) an undifferentiated program supporting proliferation by stem cells within the basal layer and 2) a differentiation program instructing growth arrest and differentiation-associated programmed cell death in suprabasal layers. (stanford.edu)
  • Epigenetic control of gene expression lasts through multiple cell divisions without alterations in primary DNA sequence and can occur via mechanisms that include histone modification and DNA methylation. (stanford.edu)
  • Noncoding RNA sequences can regulate gene expression via interactions with epigenetic and other control mechanisms. (stanford.edu)
  • RNA transport and regulated local translation play critically important roles in spatially restricting gene expression in neurons. (frontiersin.org)
  • The existence of mRNAs retaining both nuclear cap binding protein and EJC in the distal sites of neuronal processes suggests that some localized mRNAs have not yet undergone the "very first translation," which contribute to the spatio-temporal regulation of gene expression. (frontiersin.org)
  • This class of noncoding RNAs is small, single stranded, and 19-25 nucleotide long that act as negative regulators involved in posttranscriptional silencing of the gene expression [ 5 ]. (hindawi.com)
  • Due to their importance in the temporal and spatial control of gene expression, a growing number of human diseases are associated with RNAs and RBPs. (sorbonne-universite.fr)
  • Long non-coding RNAs (lncRNAs) are widely recognized as important regulators of gene expression. (mdpi.com)
  • Notably, circRNAs display cell-type, tissue-type and developmental-stage specific expression patterns in eukaryotic transcriptome, which reveals their significant regulatory functions in gene expression. (ijbs.com)
  • Alternative processing of nascent mRNAs is widespread in eukaryotic organisms and greatly impacts the output of gene expression. (stanford.edu)
  • In this review, we summarize current understanding of how APA is regulated during development and cellular differentiation and how the resulting change in 3'UTR content affects multiple aspects of gene expression. (stanford.edu)
  • For 14 cell types, we identified 26,597 independent cis-expression quantitative trait loci (eQTLs), and 62,305 trans-eQTL, with the majority showing cell type specific effects on gene expression. (iscb.org)
  • To analyze the regulatory mechanism involved in the high growth potential of C. virgata , we performed RNA-seq-based transcriptome analysis and illustrated a comprehensive gene expression map of the species. (frontiersin.org)
  • This study aimed at investigating the genomic expression during myeloid differentiation through a computational approach that integrates gene expression profiles with functional information and genome organization. (biomedcentral.com)
  • Gene expression data from 24 experiments for 8 different cell types of the human myelopoietic lineage were used to generate an integrated myelopoiesis dataset of 9,425 genes, each reliably associated to a unique genomic position and chromosomal coordinate. (biomedcentral.com)
  • For instance, the existence of tissue-specific gene clusters may be related to the efficient activation of gene expression in a particular cell lineage, by genetic and epigenetic mechanisms, or related to the repression of entire chromosomal regions containing genes expressed in a specific cell type, e.g. during the developmental switches leading to different cell lineages [ 25 ]. (biomedcentral.com)
  • The development of species-specific gene microarrays has greatly facilitated gene expression profiling in nonhuman primates. (gene-quantification.de)
  • Consequently, the time of day when RNA samples are collected can influence detection of significant changes in gene expression levels. (gene-quantification.de)
  • MicroRNAs (miRNAs) are endogenous short non-coding RNA molecules that can post-transcriptionally regulate gene expression and play a critical role in gonadal differentiation as well as early development of animals. (peerj.com)
  • immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
  • the gene expression profile during B lymphoid differentiation. (lu.se)
Genes23
  • Figure 1: Description of mRNA and Protein Variants: As mentioned previously, the specific function and however, its function can be largely inferred through the study of similar genes. (wikipedia.org)
  • An aberrant miRNA expression could contribute to cancer development and progression [ 6 , 7 ] and could affect their target genes that are involved in many biological processes, such as cell differentiation, proliferation, apoptosis, metabolism, and development [ 8 ]. (hindawi.com)
  • APA naturally occurs during development and cellular differentiation, with around 70% of human genes displaying APA in particular tissues and cell types. (stanford.edu)
  • We identified >500 Drosophila genes that express mRNA isoforms with a long 3' UTR in proliferating spermatogonia but a short 3' UTR in differentiating spermatocytes due to APA. (stanford.edu)
  • Analyzing the human transcriptome in this way, we identified more than 12,000 methylated sites in mRNA molecules derived from approximately 7,000 protein-coding genes. (pharmaceuticalintelligence.com)
  • The transcripts of most expressed genes, in a variety of cell types, were shown to be methylated, indicating that m6A modifications are widespread. (pharmaceuticalintelligence.com)
  • Gene regulatory network (GRN) inference that incorporates single-cell RNA-seq (scRNA-seq) differentiation trajectories or RNA velocity can reveal causal links between transcription factors and their target genes. (bilkent.edu.tr)
  • however, mutations in this gene and the BBS8 gene are thought to play a minor role and mutations in chaperonin-like BBS genes are found to be a major contributor to disease development in a multiethnic Bardet-Biedl syndrome patient population. (nih.gov)
  • A gain of function screen for genes that enable proliferation independently of matrix anchorage identified a cell adhesion molecule PVRL4 (poliovirus-receptor-like 4), also known as Nectin-4. (elifesciences.org)
  • explored which genes enable epithelial cells from the human mammary gland to grow without being attached to the extracellular matrix. (elifesciences.org)
  • When comparing the transcriptomes across developmental stages, we found an over-representation of genes involved in growth regulation in the early development stage in C. virgata . (frontiersin.org)
  • Results: Here, by phylogeny analysis, we found the three rab11 genes are highly conserved especially for their GTPase domains. (researchsquare.com)
  • We found that all the three genes were highly enriched in the central nervous system, but in different areas of the brain. (researchsquare.com)
  • RAB11 was first found from cultured MDCK cell cDNA library screening for homologous genes of yeast YPT1/SEC4[3], and later identified as the 11th member of the Rab family[4]. (researchsquare.com)
  • This approach allowed identifying specific chromosomal regions significantly highly or weakly expressed, and clusters of differentially expressed genes and of transcripts related to specific functional modules. (biomedcentral.com)
  • The analysis of genomic expression during human myelopoiesis using an integrative computational approach allowed discovering important relationships between genomic position, biological function and expression patterns and highlighting chromatin domains, including genes with coordinated expression and lineage-specific functions. (biomedcentral.com)
  • The correlation among co-expression and co-localization of genes was also investigated and confirmed in different organisms [ 12 ]. (biomedcentral.com)
  • The analysis of transcriptome data in the perspective of the genomic organization of genes could certainly shed light not only on the aberrations leading to pathological states but also on the physiological mechanisms of all cellular processes, including cellular differentiation. (biomedcentral.com)
  • The expression of a gene is shaped by the convergence of upstream inputs that impinge upon DNA and RNA sequence elements in the vicinity of genes, leading to precise modulation of global mRNA and protein abundances. (columbia.edu)
  • Many of these signatures are found in NP, PA, and PB2 genes (viral ribonucleoproteins [RNPs]) and are mostly located in the functional domains related to RNP-RNP interactions that are important for viral replication. (cdc.gov)
  • Although many research efforts have focused on the molecular evolution of specific genes of influenza viruses, comprehensive comparisons among the nucleotide sequences of all 8 genomic segments and among the 11 encoded protein sequences have not been extensively reported. (cdc.gov)
  • Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
  • The WT1 protein mediates the mesenchymal-epithelial transition and differentiation during morphogenesis of the kidney and gonad by repressing genes that encode cell proliferation factors and by activating genes that encode markers of epithelial cell differentiation. (medscape.com)
Apoptosis7
  • Exhibiting high consistence in sequence and structure, S100 family members are interchangeable in function and they show a wide spectrum of biological processes, including proliferation, apoptosis, migration, inflammation and differentiation and the like. (cancerindex.org)
  • However,these neurons subsequently underwent cell death through apoptosis. (biologists.com)
  • The ability of E2F-1 to prompt apoptosis in DNA damaged cells coincides with enhanced PRMT1 methylation. (embl.de)
  • In this study, we investigated the role of autophagy and apoptosis in Newcastle disease virus (NDV)-infected chicken cells and tissues. (immune-source.com)
  • In bottom line, we confirmed that autophagy brought about by genotype VII NDV infections was important for virus-like replication, NDV-induced apoptosis, and cell survival in buy 480-11-5 chicken cells and tissues. (immune-source.com)
  • Apoptosis of tumor cells increased significantly as concentration of cocultured TD-MSCs increased. (immune-source.com)
  • Animal miRNA are involved in neuronal cell fate, cell proliferation and differentiation, metabolism, aging, apoptosis and organ morphogenesis, suggest that miRNAs are particularly critical in the development, health, and aging of animals ( Ameres & Zamore, 2013 ). (peerj.com)
Single-cell RNA-seq2
  • Using single-cell RNA-seq, we identify a myeloid-biased subset within the LT-HSC population (mLT-HSCs) that is much more common amongst aged LT-HSCs and is uniquely primed to respond to acute inflammatory challenge. (biorxiv.org)
  • Single-cell RNA-seq unmasked the existence of two subsets within the LT-HSC population that was apparent upon stimulation but not steady-state. (biorxiv.org)
Subcellular localization3
  • The subcellular localization of EZH2 protein was predicted by using different predictors (CELLO, Euk-mPLoc, WoLF PSORT, and TargetP). (hindawi.com)
  • In addition, it displays dynamic subcellular localization during cellular differentiation. (sorbonne-universite.fr)
  • Furthermore, we found that RBM24 displays dynamic subcellular localization and post-transcriptional functions during muscle differentiation and regeneration. (sorbonne-universite.fr)
Proliferation8
  • Epidermal growth factor (EGF) and fibroblast growth factor 2 (FGF-2) induce the proliferation of neural precursor cells isolated from specific regions of the embryonic and adult brain. (beauty104.com.tw)
  • Not long after FGF1 and FGF2 were isolated, another group isolated a pair of heparin -binding growth factors which they named HBGF-1 and HBGF-2, whilst a third group isolated a pair of growth factors that caused proliferation of cells in a bioassay containing blood vessel endothelium cells which they called ECGF-1 and ECGF-2. (beauty104.com.tw)
  • One of the most important functions of bFGF is the promotion of endothelial cell proliferation and the physical organization of endothelial cells into tube-like structures. (beauty104.com.tw)
  • The Wnt signaling are evolutionarily conserved and implicated in cell proliferation and sarcomagenesis. (oncotarget.com)
  • Activation of Wnt/β-catenin signaling with Wnt3a or GSK-3β inhibitor drives the proliferation of bone sarcoma cells, whereas downregulation of activated Wnt signaling with dnTCF4 or siLEF1 suppresses bone sarcoma proliferation and induces cell cycle arrest. (oncotarget.com)
  • Taken together, our findings establish the evidence that aberrant activation of Wnt/β-catenin pathway involving an autocrine Wnt singaling drives the proliferation of bone sarcoma cells, and identify the autocrine activation of the Wnt/β-catenin signaling as a potential novel therapeutic target for bone sarcomas. (oncotarget.com)
  • Our results show a novel role for DDX5 in cancer cell proliferation and suggest DDX5 as a therapeutic target in breast cancer treatment. (aacrjournals.org)
  • Defects in the control of cell proliferation are a hallmark of cancer, and DNA replication is a key process for cell proliferation. (aacrjournals.org)
Embryonic5
  • The reason being: most of the embryonic tissues did not undergo appropriate cell patterning during gastrulation. (wikipedia.org)
  • Subsequently, we detected the expression of EZH2 on mRNA level and protein level in two different embryonic development stages (65-dpc and 90-dpc) via qRT-PCR and western blots. (hindawi.com)
  • Context and state of the art mRNA transport in general: Cytoplasmic mRNA transport and local translation are essential for many processes requiring symmetry breaking such as embryonic development, cell migration and neuronal differentiation (1). (europa.eu)
  • Steroid or nuclear hormone receptors constitute an important superfamily of transcription regulators that are involved in widely diverse physiological functions, including control of embryonic development, cell differentiation and homeostasis. (embl.de)
  • In primary cultures of mouse embryonic day 14 (E14) striatum, maintained in vitro for 24 hr, 12% of the cells responded to FGF-2, whereas no response to EGF could be detected. (beauty104.com.tw)
Cytoplasmic3
  • We have made the unprecedented finding that RBM24 plays a key role in cytoplasmic polyadenylation to control mRNA translation. (sorbonne-universite.fr)
  • mRNAs are transported along microtubules (MTs) by different kinesins and cytoplasmic dynein and get anchored at the actin cortex, intermediate filaments or unknown structures (1, 3). (europa.eu)
  • Since the first discovery of cytoplasmic mRNA transport almost three decades ago, several essential questions could not be answered mostly due to the complexity of the in vivo situation and the approaches available. (europa.eu)
LncRNAs2
  • Long noncoding RNAs (lncRNAs), due to their multifunction in various biological processes, have been indicated to play a crucial role in CSC renewal and stemness maintenance. (nature.com)
  • In addition, about 250 noncoding RNA sequences-including well-characterized long noncoding RNAs (lncRNAs), such as the XIST transcripts that have a key role in X-chromosome inactivation-are decorated by m 6 A. In almost all cases, the epigenetic mark was found on adenosines embedded in the predicted A/G-methylated A-C sequence. (pharmaceuticalintelligence.com)
Sequences2
  • mRNA localisation requires primary sequences and secondary structures often localised in their 3'UTR (3). (europa.eu)
  • The first aim of the study was to develop methods for the efficient and accurate identification of the involved regulatory RNA sequences and the miRNA-mRNA interactions. (fu-berlin.de)
Maturation3
  • Galectin-3, well characterized as a glycan binding protein, has been identified as a putative RNA binding protein, possibly through participation in pre-mRNA maturation through interactions with splicosomes. (bvsalud.org)
  • The process of autophagy in mammalian cells involves six principal actions: initiation, nucleation, elongation, closure, maturation, buy 480-11-5 and degradation [27]. (immune-source.com)
  • The neutrophil maturation and differentiation pathway termed myelopoiesis takes approximately 12 days. (medscape.com)
MiRNA3
  • Zu Beginn der Arbeit sollten Methoden für die exakte Identifizierung der beteiligten regulatorischen RNA-Sequenzen und der miRNA-mRNA Interaktionen entwickelt werden. (fu-berlin.de)
  • Diese Methoden ermöglichten die schnelle Amplifikation und Sequenzierung relevanter 3' UTR, sowie die Herstellung von Reporterplasmiden zur in vitro Verifikation der miRNA-mRNA Interaktion. (fu-berlin.de)
  • Die erstmalige Lokalisation ausgewählter miRNA-Familienmitglieder durch in situ Hybridisierungen im Kolon fand bei 14, 31 und 56 Tage alten Tieren statt. (fu-berlin.de)
Neurons8
  • The molecular link between splicing and mRNA localization/local translation has also been demonstrated in mammalian neurons. (frontiersin.org)
  • mRNA transport in neurons: In neurons, thousands of mRNAs are transported into axons or dendrites by so far not identified transport mechanisms (6). (europa.eu)
  • While it is clear, that RNA distribution patterns in neurons are created by active transport processes of mRNPs along microtubules, the essential enzymatic activities required are not known. (europa.eu)
  • For example, neurons tend to express mRNAs with long 3'UTRs due to preferential processing at PASs more distal than other PASs used in other cell types. (stanford.edu)
  • By contrast, Sfrs1 was not required for the survival of the neurons generated later, including later-born amacrine cells, rod photoreceptors,bipolar cells and Müller glia. (biologists.com)
  • Here, we show that the protein level of ClpP is selectively decreased in αSyn-expressing cell culture and neurons derived from iPS cells of PD patient carrying αSyn A53T mutant, and in dopaminergic (DA) neurons of αSyn A53T mice and PD patient postmortem brains. (springer.com)
  • Overexpression of ClpP reduces αSyn-induced mitochondrial oxidative stress through enhancing the level of Superoxide Dismutase-2 (SOD2), and suppresses the accumulation of αSyn S129 phosphorylation and promotes neuronal morphology in neurons derived from PD patient iPS cells carrying αSyn A53T mutant. (springer.com)
  • Seventy-five percent of these FGF-2-responsive cells were beta tubulin III (TuJ1)-positive neurons, and 25% expressed nestin, a marker for neuroepithelial precursors. (beauty104.com.tw)
Tissues13
  • Skin malignancies, including epidermal squamous cell carcinoma (SCC), alone account for nearly as many cancers as all other tissues combined. (stanford.edu)
  • We found RCC tissues displayed significantly higher miR-34a expression level than their corresponding noncancerous tissues, particularly in chromophobic subtype. (hindawi.com)
  • In addition, ORs were recently shown to be expressed in many nonolfactory tissues and cells, indicating that these receptors have physiological and pathophysiological roles beyond olfaction. (bmbreports.org)
  • Many ORs are expressed by tumor cells and tissues, suggesting that they may be associated with cancer progression or may be cancer biomarkers. (bmbreports.org)
  • ETS-1 and ETS-2 mRNA and protein levels were much higher in the ocular tissues of Tyrp-1-TAg mice than in control ocular tissues from wild-type mice. (molvis.org)
  • Although widespread, the role of APA in the biology of cells, tissues, and organisms has been controversial. (stanford.edu)
  • Mechanochemical Principles of Spatial and Temporal Patterns in Cells and Tissues. (mpi-cbg.de)
  • Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
  • A single-cell layer of epithelium is separated from the tissues beneath it by a supporting substance called the extracellular matrix. (elifesciences.org)
  • Understanding how cancerous cells gain this ability may lead to new approaches to stopping tumor cells from dividing and colonizing tissues around the body. (elifesciences.org)
  • This behavior is particularly evident in the cells that divide aggressively to form tumors that subsequently migrate and colonize other tissues around the body. (elifesciences.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • 100 HPV types, some found in skin warts and others in mucous tissues, and the association of different HPV types with cervical, some anogenital, and head and neck cancers is well established ( 3 ). (cdc.gov)
Methylation5
  • The proper methylation status of histones is essential for appropriate cell lineage and organogenesis. (hindawi.com)
  • Among the 140 modified RNA nucleotide variants identified, methylation of adenosine at the N6 position (m6A) is the most prevalent epigenetic mark in eukaryotic mRNA. (pharmaceuticalintelligence.com)
  • Identified in bacterial rRNAs and tRNAs as early as the 1950s, this type of methylation was subsequently found in other RNA molecules, including mRNA, in animal and plant cells as well. (pharmaceuticalintelligence.com)
  • We describe the residue-specific methylation of E2F-1 by the asymmetric dimethylating protein arginine methyltransferase 1 (PRMT1) and symmetric dimethylating PRMT5 and relate the marks to different functional consequences of E2F-1 activity. (embl.de)
  • In contrast, cyclin A binding to E2F-1 impedes PRMT1 methylation and augments PRMT5 methylation, thus ensuring that E2F-1 is locked into its cell-cycle progression mode. (embl.de)
Vitro5
  • The latter encompass human skin regenerated on immune deficient mice as well as organotypic constructs with epithelial and stromal cells embedded within architecturally faithful mesenchyma in vitro. (stanford.edu)
  • A recent study found that the delivery of circRNAs generated in vitro activates RIG-I-mediated innate immune responses and provides protection against viral infection. (ijbs.com)
  • Human granulosa cells (GCs) derived from women undergoing in vitro fertilization (IVF) are an adequate model for endocrine cells of the follicle and the corpus luteum and were therefore employed in an attempt to decipher their DA receptor repertoire and functionality. (biomedcentral.com)
  • Priming of striatal cells for 24 hr with FGF-2 but not with EGF was sufficient to induce the appearance of EGF- and FGF-2 responsive cells after only 2 d in vitro. (beauty104.com.tw)
  • Thus, neural precursor cells from the mouse E14 striatum initially responding to FGF-2 only acquire EGF responsiveness later during in vitro development. (beauty104.com.tw)
Molecules4
  • Modifications to RNA molecules are much more common and are critical for regulating diverse biological processes. (pharmaceuticalintelligence.com)
  • The genetic information in eukaryotic cells is stored in linear DNA molecules known as chromosomes [ 1 ]. (actanaturae.ru)
  • Shortening of the 5'-terminus of the daughter strand, caused by the removal of the terminal RNA-primer and the subsequent incomplete replication of linear DNA molecules, is observed during the genome replication occurring upon cell fission. (actanaturae.ru)
  • This can either occur by cell surface receptor interactions, as in the case with peptide growth factors, or by interactions with intracellular molecules, as in the case with steroid hormones. (beauty104.com.tw)
Eukaryotic1
  • Circular RNAs (circRNAs), a novel type of non-coding RNAs (ncRNAs), are ubiquitously expressed in eukaryotic cells during post-transcriptional processes. (ijbs.com)
Post-transcriptional1
  • MicroRNAs (miRNAs) are all non-coding RNAs that play vital role in post-transcriptional regulation of various animals and plants ( Bartel, 2009 ). (peerj.com)
Noncoding RNA1
  • The function of histone modifying epigenetic regulators and noncoding RNA as central mediators of epithelial stem cell renewal and differentiation represent major emerging areas of study in the lab. (stanford.edu)
Signaling pathway1
  • We show that TMEM53 acts as an inhibitor of BMP-SMAD signaling by preventing SMAD accumulation in the cell nucleus and that its deficiency enhances osteogenic differentiation by overactivating the BMP signaling pathway. (nature.com)
Gonadal differentiation3
  • Among the intersex disorders, pure gonadal dysgenesis with male pseudohermaphroditism is the classic presentation, although a wide variety of abnormalities in gonadal differentiation can be encountered. (medscape.com)
  • Point mutations in the WT1 gene result in loss of its regulatory function, with the consequent abnormalities in glomerular formation and gonadal differentiation seen in Denys-Drash syndrome. (medscape.com)
  • Mutations that disrupt the second alternative splicing site of the WT1 gene alter the normal ratio of KTS-positive/negative isoforms from 2:1 to 1:2 and result in abnormalities in glomerular formation and gonadal differentiation seen in Frasier syndrome. (medscape.com)
Transcriptional6
  • One sentence summary Murine hematopoietic stem cells display transcriptional heterogeneity that is quantitatively altered with age and leads to the age-dependent myeloid bias evident after inflammatory challenge. (biorxiv.org)
  • Specifically, alternative cleavage and polyadenylation (APA) is a co-transcriptional molecular process that switches the polyadenylation site (PAS) at which a nascent mRNA is cleaved, resulting in mRNA isoforms with different 3'UTR length and content. (stanford.edu)
  • We find that the pH of the nucleolus is intricately linked to the transcriptional activity of the organelle and facilitates the recruitment and condensation of DDX21. (bvsalud.org)
  • Together, our results uncover the functional diversity of HUSH targets and show that this vertebrate-specific complex exploits evolutionarily ancient transcription termination machinery for co-transcriptional chromatin targeting and genome surveillance. (bvsalud.org)
  • The regulation of expression of X-box-binding protein-1 (XBP1), a transcriptional factor, involves an unconventional mRNA splicing that removes the 26 nucleotides intron. (mdpi.com)
  • Chromatin target of Prmt1 (Chtop) is a vertebrate-specific chromatin-bound protein that plays an important role in transcriptional regulation. (embl.de)
MicroRNAs1
  • SncRNAs are less than 200 nt in length, consisting of microRNAs (miRNAs), Piwi-interacting RNAs (piRNAs), endogenous small interfering RNAs (endo-siRNAs or esiRNAs) and transcription initiation RNAs (tiRNAs) [ 6 ]. (ijbs.com)
Cellular6
  • Analyses of RBM24 loss of function reveal that it is critically required for cellular differentiation. (sorbonne-universite.fr)
  • While most current platforms for spatial transcriptomics only provide multi-cellular resolution (10-15 cells per spot), recent technologies provide a much denser spot placement leading to sub-cellular resolution. (bilkent.edu.tr)
  • Analysis of RNAs enriched in different sub-cellular regions based on SCS spot assignments provides information on RNA localization and further supports the segmentation results. (bilkent.edu.tr)
  • Murine Rab11a and Rab11b were found to localize in distinct cellular compartment, suggesting functional differentiation [9]. (researchsquare.com)
  • Human myelopoiesis is an exciting biological model for cellular differentiation since it represents a plastic process where multipotent stem cells gradually limit their differentiation potential, generating different precursor cells which finally evolve into distinct terminally differentiated cells. (biomedcentral.com)
  • Other cellular components of the innate immune system include mononuclear phagocytes, dendritic cells, and NK cells. (medscape.com)
Interactions5
  • APA can potentially affect mRNA translation efficiency, localization, stability, and mRNA seeded protein-protein interactions. (stanford.edu)
  • These motions, referred to as intrinsic dynamics, ensure the adaptation to various interactions in the cell, and largely assist in, if not determine, viable mechanisms of biological function. (bilkent.edu.tr)
  • Spatial transcriptomics promises to greatly improve our ability to understand tissue organization and cell-cell interactions. (bilkent.edu.tr)
  • also showed that interactions between the PVRL4 in one cell and a related protein called PVRL1 in a neighboring cell were responsible for holding the cells together in clusters. (elifesciences.org)
  • Cell-to-cell interactions set off a cascade of events that may result in T- or B-cell activation and, ultimately, host defense. (medscape.com)
Neuronal2
  • Here we report a class of mRNA granules in human neuronal processes that are enriched in the nuclear cap-binding protein complex (CBC) and exon junction complex (EJC) core components, Y14 and eIF4AIII. (frontiersin.org)
  • Neuronal mRNA transport occurs in packages of single to a few copies of mRNAs (8-11), mostly in a translationally repressed mode. (europa.eu)
Subsequently2
  • The cells subsequently enter a state of crisis, accompanied by massive cell death. (actanaturae.ru)
  • We subsequently show how eQTLs have dynamic allelic effects in B cells transitioning from naïve to memory states, and demonstrate how commonly segregating alleles lead to inter-individual variation in immune function. (iscb.org)
Nucleotide3
Functional6
  • NF90/NF110 are also functional in inhibiting viral replication through binding to viral mRNAs. (ijbs.com)
  • Although the identification of m6A in RNA is 40 years old, until recently researchers lacked efficient molecular mapping and quantification methods to fully understand the functional implications of the modification. (pharmaceuticalintelligence.com)
  • However, with limited functional evidence of a direct RNA interaction, many molecular-level observations rely on affinity reagents and lack appropriate genetic controls. (bvsalud.org)
  • Bioincompatible dialysis fluid causes mesothelial cells to undergo uncontrolled mesothelial-to-mesenchymal transition triggered by Transforming growth factor-β1, which leads morphological and functional disruption of membrane. (ukessays.com)
  • In this study we used phosphorylation of the transcription factor cAMP response element-binding protein as a functional readout to identify cells responding to EGF and FGF-2. (beauty104.com.tw)
  • Of these, three miRNAs were differentially expressed miRNAs and 18 miRNAs involved in sexual differentiation as determined by functional analysis with GO annotation and KEGG pathway analysis. (peerj.com)
Transcriptome2
  • However, it has been proven that more than 62% of genomic DNA serves as a template for transcription, which indicates that there are abundant non-coding RNAs (ncRNAs) in human transcriptome [ 2 , 3 ]. (ijbs.com)
  • Through de novo transcriptome assembly with the RNA-seq reads from whole organ samples of C. virgata at the germination stage (2 days after germination, DAG), early young development stage (8 DAG), young development stage (17 DAG), and adult development stage (28 DAG), we identified 21,589 unified transcripts (contigs) and found that 19,346 and 18,156 protein-coding transcripts were homologous to those in rice and Arabidopsis , respectively. (frontiersin.org)
Biology1
  • Given recent developments with cell surface RNA biology, the putative dual-function nature of galectin-3 evokes a possible non-classical connection between glycobiology and RNA biology. (bvsalud.org)
Endogenous1
  • We demonstrate that antibodies raised to endogenous human galectin-3 can isolate RNA-protein crosslinks, but this activity remains insensitive to LGALS3 knock-out. (bvsalud.org)
Situ hybridization1
Exons4
  • These unique mRNAs differ by varying truncation of the 3' and 5' ends, as well as the presence of 3 cassette exons. (wikipedia.org)
  • Circular RNAs (circRNAs), a novel member of the lncRNA kingdom, are generated by non-sequential backsplicing of exons, introns or a combination of both [ 7 - 9 ]. (ijbs.com)
  • The few mutations reported so far resemble those commonly found in AS, being located in the three largest exons (exons 8, 10 and 16) and predicted to cause premature termination of translation. (springer.com)
  • 2, 3, 4, 5] The WT1 gene contains 10 exons that produce 4 different messenger RNAs (mRNAs) as a result of 2 alternative splicing sites in exons 5 and 9 that, in turn, encode 4 different isoforms of the WT1 protein. (medscape.com)
Nascent2
  • Developmentally regulated alternate 3' end cleavage of nascent transcripts controls dynamic changes in protein expression in an adult stem cell lineage. (stanford.edu)
  • We further demonstrate that HUSH interacts with the termination factor WDR82 and-via its component MPP8-with nascent RNA. (bvsalud.org)
Epithelial cell1
  • We find that the DDX5 locus is frequently amplified in breast cancer and that breast cancer-derived cells with amplification of DDX5 are much more sensitive to its depletion than breast cancer cells and a breast epithelial cell line that lacks DDX5 amplification. (aacrjournals.org)
Subsequent1
  • The inactivation lead to abnormal cell positioning and subsequent death. (wikipedia.org)
Processes3
  • DOP1B has been found to be involved in the following processes: multicellular organism development in cell differentiation and developmental patterning, cognition, as well as endoplasmic reticulum organization and Golgi to endosome transport. (wikipedia.org)
  • DEAD-box ATPases are major regulators of biomolecular condensates and orchestrate diverse biochemical processes that are critical for the functioning of cells. (bvsalud.org)
  • All through the development process of peritoneal fibrosis, peritoneal cells will experience changes and some of the pro inflammatory factors which act as inducers to induce fibrosis and inflammatory processes. (ukessays.com)
Dendritic1
  • Dendritic localisation of CaMKIIa-mRNA requires its 3'UTR harbouring binding sites for RBPs as FMRP and Staufen2, which are required for its correct localisation. (europa.eu)
Regulation3
  • In addition to classical gene regulatory networks noted above, we have recently identified a central role for additional biologic mechanisms, namely gene regulation by chromatin regulators and by noncoding RNAs. (stanford.edu)
  • During meiosis, germ cell and stage-specific components impose additional layers of regulation on the core cell cycle machinery to set up an extended G2 period termed meiotic prophase. (stanford.edu)
  • Regulation and function of alternative polyadenylation in development and differentiation. (stanford.edu)
Variants3
  • Transcription produces 10 unique mRNAs, 8 alternatively spliced variants, and 2 unspliced forms. (wikipedia.org)
  • These mRNA variants range from 7691bp (mRNA variant DOPEY2.aAug10) to 315bp (mRNA variant DOPEY2.jAug10-unspliced) and are further described in Table 1 below. (wikipedia.org)
  • To date, a lot of EZH2 variants have been found in various cell and tissue types [ 18 - 20 ]. (hindawi.com)
Interaction3
  • We also applied Velorama to study cell differentiation in pancreas, dentate gyrus, and bone marrow from real datasets and obtained intriguing evidence for the relationship between regulator interaction speeds and mechanisms of gene regulatory control during differentiation. (bilkent.edu.tr)
  • Genetic ablation of HNRNPA2B1, but not LGALS3, eliminates the ability of the anti-galectin-3 antibodies to isolate RNA-protein crosslinks, implying either an indirect interaction or cross-reactivity. (bvsalud.org)
  • cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)
Biol2
Squamous cell carc3
  • A growing body of research shows that human papillomavirus (HPV) is a common and increasing cause of oropharyngeal squamous cell carcinoma (OSCC). (cdc.gov)
  • We focus on the role of HPV in the increased incidence of oropharyngeal squamous cell carcinoma (OSCC), the head and neck cancer in which HPV is most commonly found ( 4 ). (cdc.gov)
  • Head and neck cancer most commonly is of the squamous cell carcinoma type (HNSCC) and includes cancers of the oral cavity, oropharynx, hypopharynx, larynx, sinonasal tract, and nasopharynx. (cdc.gov)
Highly conserved1
  • The RNA-binding protein RBM24 displays highly conserved expression pattern in vertebrate embryos. (sorbonne-universite.fr)
Mechanisms2
  • Mechanisms regulating cardiomyocyte cell cycle arrest are of great interest partly because reversing this process could provide a way to stimulate cardiac regeneration after injury [ 17 ]. (springer.com)
  • Our goal is to convey a picture of pattern formation that draws attention to the principles rather than solely to specific molecular mechanisms. (mpi-cbg.de)
Degradation1
  • In the canonical Wnt/β-catenin signaling, a Wnt ligand binds to a cell surface receptor complex consisting of the members of Frizzled family and the transmembrane protein LRP 5 and 6 to prevent phosphorylation and degradation of β-catenin by the GSK3β/APC/Axin destruction complex. (oncotarget.com)
Tumor7
  • Tumor initiation, development, and relapse may be closely associated with cancer stem cells (CSCs). (nature.com)
  • and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)
  • In addition to basic science investigations in such areas as tumor cell invasion, oncogenes, immunotherapy and genetic inheritance, our scientists and physicians participate in national cancer trials. (upstate.edu)
  • During all stages of tumor progression, cancer cells are subjected to inappropriate extracellular matrix environments and must undergo adaptive changes in order to evade growth constraints associated with the loss of matrix attachment. (elifesciences.org)
  • 5 Functionally, proinflammatory polarization leads to potent effector cells that kill intracellular micro-organisms and tumor cells. (haematologica.org)
  • Objectives Mesenchymal stem cells (MSCs) play an important role in the development and growth of tumor cells. (immune-source.com)
  • Cell cycle analysis showed an accumulation of tumor cells predominantly in 511296-88-1 manufacture G0/G1 phase with an increase in concentration of TD-MSCs, which was confirmed by increased mRNA expression of cell cycle negative regulator p21. (immune-source.com)
Receptors8
  • High levels of dopamine (DA) were described in human ovary and recently evidence for DA receptors in granulosa and luteal cells has been provided, as well. (biomedcentral.com)
  • However, neither the full repertoire of ovarian receptors for DA, nor their specific role, is established. (biomedcentral.com)
  • The presence of the trophic hormone hCG (10 IU/ml) in the culture medium for several days did not alter mRNA (semiquantitative RT-PCR) or protein levels (immunocytochemistry/Western blotting) of D 1,2,4,5 DA receptors. (biomedcentral.com)
  • Among the DA receptors found in human GCs, D 2 expression was marked both at mRNA and protein levels and it was therefore further studied. (biomedcentral.com)
  • A previous study showed D 2 in human GCs, which are linked to cAMP, and the present study reveals the full spectrum of DA receptors present in these endocrine cells, which also includes D 2 -like receptors, linked to calcium. (biomedcentral.com)
  • Here we demonstrate aberrant activation of Wnt/β-catenin signaling in bone sarcoma cells, involving an autocrine Wnt signaling loop with upregulation of specific Wnt ligands and receptors. (oncotarget.com)
  • The generation of receptors specific for antigens is a unique and complex process that generates 10 12 specific receptors for each cell type of the adaptive immune system, including T and B cells. (medscape.com)
  • Various cell types can process and present these antigens to T cells, or antigens may be soluble and bound to B-cell receptors. (medscape.com)
Progression2
  • Immune cells that infiltrate lesions are important for atherosclerosis progression and immunotherapies. (biomedcentral.com)
  • We identify an important role for DDX5 in G 1 -S-phase progression where it directly regulates DNA replication factor expression by promoting the recruitment of RNA polymerase II to E2F-regulated gene promoters. (aacrjournals.org)
Molecular2
  • In this context, we have been engaged in understanding the molecular events during muscle cell development in vertebrate embryos. (sorbonne-universite.fr)
  • Understanding the molecular basis of AS is critical for the development of specific therapies. (springer.com)
Evolutionarily2
  • The evolutionarily conserved RBM24 (RNA-Binding Motif Protein 24) is highly expressed in muscles and head sensory organs of all vertebrate embryos. (sorbonne-universite.fr)
  • We identify an evolutionarily conserved feature of the C terminus of DDX21 responsible for nucleolar localization. (bvsalud.org)
Reveal2
  • Isolation of the tagged galectin-3 failed to reveal any RNA-protein crosslinks. (bvsalud.org)
  • Understanding how DNA replication is regulated in human cells can provide insight into cancer development and may reveal vulnerabilities that can be exploited therapeutically. (aacrjournals.org)
Stem cells2
  • Long-term hematopoietic stem cells (LT-HSCs) maintain hematopoietic output throughout an animal's lifespan. (biorxiv.org)
  • Stem cells are characterized by a lower telomerase activity, which enables only partial compensation for the shortening of telomeres. (actanaturae.ru)
Viral genomes2
  • Position-specific entropy profiles created from scanning 306 human and 95 avian influenza A viral genomes showed that 228 of 4,591 amino acid residues yielded significant differences between these 2 viruses. (cdc.gov)
  • In this study, we used several computational approaches for finding specific genetic signatures characteristic of human and avian influenza A viral genomes. (cdc.gov)
Distal1
  • After induction of long-term potentiation, CaMKIIa-mRNA is transported to distal parts of dendrites where its translation is locally regulated (12, 13). (europa.eu)
Biological2
  • although it is derived from cells of the renal tubular epithelium, it has several histological subtypes which differ in their clinical outcome and biological features. (hindawi.com)
  • This study was designed to examine whether the unconventional spicing of XBP1 mRNA could occur in the nucleus and its possible biological relevance. (mdpi.com)
Extracellular5
  • COMPARTMENTS localization data places the highest confidence of localization to the extracellular exosome and the Golgi membrane. (wikipedia.org)
  • It is estimated that up to 90% of cancers in humans originate in epithelial tissue, and the cells within such tumors are known to survive and divide even when they are no longer attached to the extracellular matrix. (elifesciences.org)
  • Moreover, cancerous cells tend to cluster together when they are detached from the extracellular matrix. (elifesciences.org)
  • Moreover, PVRL4 triggers a form of signaling between the cells called integrin β4 signaling that allows them to survive without being anchored to the extracellular matrix. (elifesciences.org)
  • In conclusion, we provide evidence that proinflammatory polarization of macrophages does not lead to enhanced procoagulatory function, whereas alternative polarization of macrophages leads to an increased expression of TF and increased production of TF bearing extracellular vesicles by these cells suggesting a procoagulatory phenotype of alternatively polarized macrophages. (haematologica.org)
Regulatory2
  • We are currently pursuing studies of the dominant signaling and gene regulatory networks that control this process, including the Ras/MAPK cascade, which is required for stem cell-mediated self-renewal and the p53 transcription factor family member, p63, which is required for epidermal differentiation. (stanford.edu)
  • It is involved in p53 pathways and is implicated in cell death/survival signaling, the cell cycle, and differentiation, thereby playing a regulatory role in carcinogenesis [ 12 ]. (hindawi.com)
Repression2
  • Previous work showed that the cell cycle regulator Cyclin B (CycB) is subject to translational repression in immature spermatocytes, mediated by the RNA-binding protein Rbp4 and its partner Fest. (stanford.edu)
  • Here we show that the spermatocyte-specific protein Lut is required for translational repression of cycB in an 8-hour window just before spermatocytes are fully mature. (stanford.edu)
Genetic1
  • used genetic techniques to silence PVRL4 in cells from breast tumors, they found that it reduced the formation of clusters by the cancer cells and also reduced their ability to grow in the absence of attachment. (elifesciences.org)
Polyadenylation2
  • We will focus our research on the identification of its interacting partners and mRNA targets to understand how it regulates mRNA stability, polyadenylation and translation. (sorbonne-universite.fr)
  • Alternative polyadenylation (APA) generates transcript isoforms that differ in the position of the 3' cleavage site, resulting in the production of mRNA isoforms with different length 3' UTRs. (stanford.edu)
Expression9
  • The mRNA expressed and levels of expression differ based on the location and tissue type in the body, but overall has been found to be expressed ubiquitously. (wikipedia.org)
  • The highest expression has been found in differentiating, rather than proliferating, tissue zones. (wikipedia.org)
  • Here, we show that young and aged LT-HSCs respond differently to inflammatory stress, such that aged LT-HSCs produce a cell-intrinsic, myeloid-biased expression program. (biorxiv.org)
  • These analyses of the relationships between patient survival and expression patterns obtained from an open mRNA database in cancer patients indicate that ORs may be cancer biomarkers and therapeutic targets. (bmbreports.org)
  • In addition, OR expression pattern in various cancers were analyzed based on RNA-sequencing data reported in the Cancer Genome Atlas (TCGA), and the associations between patient survival outcomes and OR levels were analyzed to determine the clinical relevance and significances of OR expression in tumors. (bmbreports.org)
  • In addition, spermatocyte-specific isoforms of Syncrip (Syp) are required for expression of CycB protein in mature spermatocytes and normal entry into the meiotic divisions. (stanford.edu)
  • In addition, changes in APA mark a variety of pathological states, including many types of cancer, in which mRNAs are preferentially cleaved at more proximal PASs, causing expression of mRNA isoforms with short 3'UTRs. (stanford.edu)
  • Additionally, mRNA expression of caspase 3 was upregulated with increased concentration of TD-MSCs. (immune-source.com)
  • B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)
MiRNAs1
  • Although sexual dimorphism is most visual in birds (example peacock and peahen, hummingbirds, songbirds, paradise fly-catcher), the miRNAs involved in gender differentiation among Psittaciformes are not known. (peerj.com)
Antibodies1
  • During my PhD studies at the Indian Institute of Science (2001-2007), I made a groundbreaking discovery by identifying neutralizing antibodies against Abrin, a highly potent RNA-degrading protein. (westminster.ac.uk)
Nuclear1
  • Driven by a profound interest in the nuclear events that influence human cell health and disease, I dedicated four years to serving as a European Framework for DNA Repair Postdoctoral Research Fellow. (westminster.ac.uk)