Loading...
  • mRNA
  • In osteogenic media, cells pretreated with 300nM reversine exhibited an increased induction in alkaline phosphatase activity and enhanced expression of alkaline phosphatase, bone sialoprotein, osteocalcin, and collagen type I mRNA. (bjmu.edu.cn)
  • antibodies
  • CD19 has been targeted through genetically engineered chimeric-antigen receptor (CAR) T cells, and via bispecific CD3-CD19 antibodies, with reports of relapse via CD19 loss. (nih.gov)
  • dysfunction
  • Conversely, the constitutive activation of calcineurin or calmodulin, a Ca 2+ binding protein, also causes marked β-cell dysfunction ( 3 , 10 , 11 ). (diabetesjournals.org)
  • However, beta cell dysfunction in Wolfram syndrome has yet to be fully characterised, and there are also no specific treatment recommendations. (springer.com)
  • Our study provides deeper insights into the pathophysiology of beta cell dysfunction caused by WFS1 deficiency and implies that activation of the GLP-1 receptor signal may alleviate insulin insufficiency and aid glycaemic control in Wolfram syndrome. (springer.com)
  • dynamics
  • Our new special issue is packed with articles that use mathematical and physical approaches to gain insights into cell and tissue patterning, morphogenesis and dynamics, and that provide a physical framework to capture these processes operating across scales. (biologists.org)
  • Ca 2+ is a ubiquitous second messenger that is central to regulating cellular dynamics of many cell types, including β-cells. (diabetesjournals.org)
  • In addition, granule dynamics in living beta cells were examined using total internal reflection fluorescence microscopy. (springer.com)
  • Induction
  • Effect of Genotype, Explant Type and 2,4-D on Cell Dedifferentiation and Callus Induction in Cumin ( Cuminum cyminum L.) Medicinal Plant', Journal of Applied Biotechnology Reports , 2(3), pp. 265-270. (biotechrep.ir)
  • In chondrogenic media, cells pretreated with reversine exhibited marked increase in the induction of aggrecan, collagen types II, IX, and XI, and versican. (bjmu.edu.cn)
  • phenotypic
  • With time distinct neointimal thickening occurs, associated with disappearance of proliferative activity and a phenotypic shift of the smooth muscle cells. (bmj.com)
  • fibroblasts
  • Generation of human induced pluripotent stem cells from dermal fibroblasts," Proceedings of the National Academy of Sciences of the United States of America , vol. 105, no. 8, pp. 2883-2888, 2008. (hindawi.com)
  • Oct4
  • Oct4 induced dedifferentiation was associated with endogenous Oct4 promoter activity, expression of Nanog and Klf4, and global gene expression changes that enriched for transcription factors. (aacrjournals.org)
  • Furthermore, ShRNA mediated knockdown of Oct4 in dedifferentiated cells led to diminished CSC phenotypes. (aacrjournals.org)
  • pluripotency
  • the reprogramming ESC is called induced pluripotency stem cell (iPSC) [ 5 ]. (ijbs.com)
  • 19755138 ). Plays a role, in a LIF-independent manner, in maintainance of self-renewal and pluripotency of embryonic and trophoblast stem cells through different signaling pathways including FGF signaling pathway and Wnt signaling pathways. (uniprot.org)
  • chimeric antigen receptor
  • The success of autologous chimeric antigen receptor (CAR) T-cell therapy in patients with hematologic malignancies, particularly CD19 + B-cell acute lymphoblastic leukemia (B-ALL), has been partially mitigated by the development of severe side effects, such as neurotoxicity and cytokine release syndrome, in a subset of patients. (aacrjournals.org)
  • inflammation
  • The preventive effect of FoxO ablation on atherosclerosis likely ensues from its ability to increase cell survival and nitric oxide availability, and dampen inflammation, chemotaxis, and superoxide generation. (nbdiabetes.org)
  • insulin
  • Type 1 diabetes (T1D) is a chronic disease characterized by an autoimmune-mediated destruction of insulin-producing pancreatic β cells. (jci.org)
  • Recent data suggest that viral infection of human islets leads to a decrease in insulin production rather than β cell death, suggesting loss of β cell identity. (jci.org)
  • Arrowheads point to insulin + (INS + ) cells that stain positive for SOX9 following cytokine treatment. (jci.org)
  • You take cells from a patient who's been totally insulin-dependent for 20 years and in the lab those cells are making insulin. (nbdiabetes.org)
  • The Accili laboratory is interested in insulin action and pancreatic-cell function. (nbdiabetes.org)
  • The mechanisms by which chronic metabolic stress, including insulin resistance, glucotoxicity, and lipotoxicity ( 1 - 3 ), impair β-cell function are not understood. (diabetesjournals.org)
  • The number of insulin granule fusion events in the first phase decreased by 41% in Wfs1 −/− beta cells compared with WT beta cells. (springer.com)
  • transcriptional program
  • In the peripheral nervous system, Schwann cells (SCs) surrounding damaged axons undergo an injury response that is driven by an intricate transcriptional program and is critical for nerve regeneration. (nih.gov)
  • characteristic
  • Ultrastructurally, the characteristic secretory granules in the neoplastic cells decreased remarkably in number, consistent with the immunohistochemical findings. (springer.com)
  • somatic
  • The H3K27me3 is a repressive chromatin marker in dedifferentiating somatic cells to iPSCs. (ijbs.com)
  • The H3K27me3 domains in iPSC are smaller, interspersed, and spanning less base pairs as compared with somatic cells [ 14 ]. (ijbs.com)
  • Cellular
  • Further analysis revealed that there was involvement of gene networks concerned with cellular assembly and organization, DNA replication and repair, tissue morphology, and cell-to-cell signaling. (bjmu.edu.cn)
  • Plant cells have a remarkable plasticity that allows cellular reprogramming from differentiated cells and subsequent tissue regeneration. (bioportfolio.com)
  • morphology
  • Reversine modulated cell growth, morphology, and the actin cytoskeleton of annulus fibrosus cells. (bjmu.edu.cn)
  • Taken together, our results suggest that chronically elevated β-cell [Ca 2+ ] i in Abcc8 −/− islets contributes to the alteration of β-cell identity, islet cell numbers and morphology, and gene expression by disrupting a network of Ca 2+ -regulated genes. (diabetesjournals.org)
  • expression
  • Microarray profiling coupled with Ingenuity Pathway Analysis revealed that reversine treatment resulted in a significant expression change in many genes including those required for cell-cell interaction, cell movement, cell growth, and development. (bjmu.edu.cn)
  • It can be induced by nuclear transfer, cell fusion, or forced transcription factors expression [ 1 , 2 ]. (ijbs.com)
  • A pulse of C/EBPα followed by an over expression of OSKM permits a rapid and highly efficient reprogramming of B cells into iPSCs [ 16 ]. (ijbs.com)
  • Expression is required in OSN to cell-autonomously regulate OSN axon projections. (uniprot.org)
  • Also regulates expression of multiple rod-specific genes and is required for survival of this cell type (By similarity). (uniprot.org)
  • NLRP3 expression in PVN was increased significantly in prehypertensive rats, accompanied by increased number of microglia, CD4 + , CD8 + T cell, and CD8 + microglia. (springermedizin.de)
  • RNA-sequencing analysis of FACS-purified Abcc8 −/− β-cells confirmed an increase in Ppy gene expression and revealed altered expression of more than 4,200 genes, many of which are involved in Ca 2+ signaling, the maintenance of β-cell identity, and cell adhesion. (diabetesjournals.org)
  • secretion
  • SOX9 was induced by the NF-κB pathway and also in a paracrine non-cell-autonomous fashion through the secretion of IFN-α. (jci.org)
  • Furthermore
  • Furthermore, only a few neoplastic cells in specimens obtained at postmortem sampling exhibited a weak response to CT. (springer.com)
  • regulates
  • TOR (focus on of rapamycin) can be an evolutionarily conserved nutrient sensing proteins kinase that regulates development and metabolism in every eukaryotic cells. (expandingmarketing.com)
  • Regulates non-cell-autonomously the layer formation of the olfactory bulb development and the interneurons. (uniprot.org)
  • formation
  • Cell implantation markedly improved vascularity and cardiac function after myocardial infarction without any evidence of teratoma formation. (ahajournals.org)
  • Callus formation occurs from pericycle-like cells through a la. (bioportfolio.com)
  • Previously, we have demonstrated that Sex-determining region Y-box 9 (SOX9) is ectopically expressed during activation of hepatic stellate cells (HSC) when it is responsible for the production of type 1 collagen, which causes scar formation in liver fibrosis. (semanticscholar.org)
  • role
  • HEFCE-funded clinician scientist who has played an integral role in fostering the multidisciplinary networks underpinning translation of cell therapy in Newcastle. (ncl.ac.uk)
  • receptor
  • In this study, we aimed to characterise beta cell secretory defects and to examine the potential effects of a glucagon-like peptide-1 (GLP-1) receptor agonist on diabetes in Wolfram syndrome. (springer.com)
  • CD19
  • Although recent studies have identified potential biomarkers for the development of these significant toxicities, particularly for the CD19 CAR 19-41BB T-cell product, currently it is unknown whether these biomarkers and cytotoxicities are CAR construct-specific. (aacrjournals.org)
  • acute lymphobl
  • Novel treatments for acute lymphoblastic leukemia (ALL) are utilizing the patient's T cells targeting surface molecules on the leukemic blasts. (nih.gov)
  • sufficient
  • If sufficient cells were present in the culture inoculate, a continuous monolayer formed at about 9 days of culture and redifferentiation of smooth muscle began. (biologists.org)