Cell death in hela cells. Medical search. Frequent questions

Henrietta Lacks was an East Baltimore resident and cervical cancer patient in the early 1950s at The Johns Hopkins Hospital, where cells taken from her tumor became the first "immortal" human cells grown in culture and have led to breakthroughs in cell research related to cancer, AIDS, the effects of radiation and more. (jhu.edu)
They are called 'HeLa' cells from their initial host's name, Henrietta Lacks. (philosophynow.org)
So back to Henrietta Lacks and her overly hardy-some say virulent-cervical cancer cells. (candidaabrahamson.blog)
Hela cells, otherwise known as Henrietta Lacks' HeLa cells, were taken from a sample of her cervical cancer cells in 1951 without her knowledge or consent and were subsequently used to create the first ever polio vaccine. (helacells.com)
Her name was Henrietta Lacks, but scientists know her as HeLa. (worldcat.org)

We treated TMZ-resistant cells U251-TMZ and T98G-TMZ to normoxia or hypoxia and silenced NUPR1 in hypoxia-treated U251-TMZ and T98G-TMZ cells to assess cell viability, proliferation, apoptosis, LC3-II/LC3-I and p62 expressions, and autophagic flux under different concentrations of TMZ. (techscience.com)
Also, our mutants induced proliferation, glucose uptake, inhibited the migration of 293T cells and affected the responsiveness of the cells to cisplatin and radiation. (scielo.br)
During the metastasis, cancer cells in situ move to other tissues and organs from the blood circulation or the lymphatic system through proliferation, adhesion, invasion, and migration. (frontiersin.org)
The proliferation activity, mortality of SH-SY5Y cells, adenosine triphosphate and pyruvic acid were measured. (ijpsonline.com)
The Effect of Vitamin C on HeLa Cell Proliferation" by James D. Dinger, Allysa C. Steinhaus et al. (csp.edu)
The results showed that a reduction in U251 cell proliferation was associated with the regulation of JAK2/STAT3 and p38 MAPK/ERK signaling pathways. (bvsalud.org)
Effects of Ganoderma lucidum spores on HepG2 cells proliferation and growth cycle. (springer.com)
Results obtained indicate that both cannabidiol and Cannabis sativa extracts were able to halt cell proliferation in all cell lines at varying concentrations. (biomedcentral.com)
These compounds are almost structurally similar or possess identical pharmacological activities and offer various potential applications including the ability to inhibit cell growth, proliferation and inflammation [ 22 ]. (biomedcentral.com)
In previous research, the authors demonstrated that the methanol extract of Artemisia vulgaris (AVM) has the ability to inhibit chronic myeloid leukemia (CML) cell proliferation. (spandidos-publications.com)
Sesquiterpene lactones containing a 3-methylenedihydrofuran-2(3H)-one (or α-methylene-γ-lactone) moiety, for example damsin (1), are Michael acceptors that affect biological processes such as cell proliferation, death/apoptosis, and cell migration, by interfering with cell signalling pathways. (lu.se)

Therefore, in this review, we avoid a lengthy repetition of the description of autophagy and cell death processes and focus on the death-promoting roles of autophagy and the intertwined connection between autophagy and apoptosis. (nature.com)
In the following article, you'll see that researchers have found both ionic silver and metallic silver to cause cervical cancer cells to commit cellular suicide through a process called apoptosis. (thesilveredge.com)
According to the study abstract, the researchers found that silver nanoparticles caused the cervical cancer cells to self-destruct, in a process called apoptosis . (thesilveredge.com)
Apoptosis…was associated with this cell death. (thesilveredge.com)
In other words, in this test-tube study, when a source of silver ions was used on the HeLa cells line (i.e., cervical cancer cell line) the cancer cells committed cellular suicide (apoptosis) at a higher rate than when the same cancer cells were exposed to metallic silver. (thesilveredge.com)
Since cells with uncompromised membranes may retain esterase activity and an early stage of apoptosis is cessation of metabolic activity, electrochemical metabolic activity measurements may be an early indicator of programmed cell death. (nist.gov)
Glucose withdrawal induces cell death that can occur by apoptosis (in Bax, Bak-deficient mouse embryonic fibroblasts or HeLa cells) or by necrosis (in Rh4 rhabdomyosarcoma cells). (gla.ac.uk)
Inhibition of autophagy by chemical or genetic means by using 3-methyladenine, chloroquine, a dominant negative form of ATG4B or silencing Beclin-1, Atg7, or p62 indicated that macroautophagy does not protect cells undergoing necrosis or apoptosis upon glucose deprivation. (gla.ac.uk)
It was previously demonstrated that redirection of Bcl-x pre-mRNA splicing from Bcl-xL to -xS induced apoptosis in breast and prostate cancer cells. (unc.edu)
Mcl-1 splice-switching resulted in PARP cleavage and cell death in HeLa cells, indicative of apoptosis. (unc.edu)
These findings validate Mcl-1 as a target for SSOs to induce apoptosis and sensitize cancer cells to chemotherapy. (unc.edu)
The cytotoxicity, intracellular reactive oxygen species (ROS), matrix metalloproteinase (MMP), and cell apoptosis activity of TA have been evaluated by the MTT assay and by DCFH-DA, Rh-123, AO/EB, and western blot analysis. (bvsalud.org)
The results obtained showed that TA abridged the viability of U251 cells, while MMP increased apoptosis by increasing the ROS levels in a time-dependent manner. (bvsalud.org)
Our findings showed that the suppression of JAK2/STAT3 and p38 MAPK/ERK signaling by TA results in cell growth arrest and stimulation of apoptosis in GBM cells. (bvsalud.org)
Khz inhibited cell division and induced apoptosis in A549 cells. (springer.com)
Induction of apoptosis by ethanol extracts of Ganoderma lucidum in human gastric carcinoma cells. (springer.com)
Ganoderic acid Mf and S induce mitochondria mediated apoptosis in human cervical carcinoma HeLa cells. (springer.com)
AVM induced the release of cleaved PARP and cleaved caspase‑3 caused apoptosis and inhibited the viability of these cells. (spandidos-publications.com)

Role of eukaryotic translation initiation factor 4E (eIF4E) in cadmium -induced cytotoxicity and cell death. (cdc.gov)
The role of eukaryotic translation initiation factor 4E (eIF4E), in cadmium -induced cytotoxicity and cell death was investigated. (cdc.gov)
The SiRNAmediated silencing of eIF4E gene expression resulted in significant cytotoxicity and cell death suggesting that the cytotoxicity and cell death noticed among the Cdtreated cells were probably due to the chemical-induced inhibition of eIF4E gene expression. (cdc.gov)
Transgenic Chinese hamster ovary cell lines overexpressing eIF4E were resistant to Cd-induced cytotoxicity and cell death. (cdc.gov)
Pre-exposure of cells to proteasome inhibitors blocked the Cd-induced inhibition of eIF4E gene expression as well as the resulting cytotoxicity and cell death. (cdc.gov)
Taken together, our results demonstrate that the exposure of cells to cadmium chloride resulted in cytotoxicity and cell death due to the enhanced ubiquitination and proteolysis and the consequent inhibition of eIF4E gene expression leading to diminished cellular level of critical genes such as cyclin D1. (cdc.gov)
After adding vitamin C reagent to plated HeLa cells, we performed cytotoxicity assays to measure cell viability. (csp.edu)
Khz cytotoxicity was measured using an MTT assay and the mitochondrial membrane potential (MMP)-related, calcium-induced generation of reactive oxygen species (ROS) in A549 cells was measured by flow cytometry. (springer.com)
Miyajima, A, Nakashima J, Yoshioka K. Role of reactive oxygen species in cis-dichlorodiammineplatium-induced cytotoxicity on bladder cancer cells. (springer.com)
In this investigation, the cytotoxicity of 23 α-methylene-γ-lactones towards normal breast epithelial MCF-10A cells as well as breast cancer JIMT-1 cells is compared. (lu.se)

In this study we compare the anti-proliferative effects of crude extract of Cannabis sativa and its main compound cannabidiol on different cervical cancer cell lines. (biomedcentral.com)
In conclusion, these data suggest that cannabidiol rather than Cannabis sativa crude extracts prevent cell growth and induce cell death in cervical cancer cell lines. (biomedcentral.com)
Therefore, the aim of this study was to evaluate the cytotoxic and anti-proliferative properties of Cannabis sativa and its isolate, cannabidiol in cervical cancer cell lines. (biomedcentral.com)

Western blot analysis of the cells demonstrated a significant inhibition of eIF4E gene (protein) in response to Cd exposure. (cdc.gov)
Whether the inhibition of eIF4E was responsible for the observed toxicity and death was studied by silencing the cellular expression of eIF4E gene by employing a small interfering RNA (SiRNA) specifically targeting the eIF4E gene. (cdc.gov)
Furthermore, exposure of cells to Cd resulted in a significant inhibition of expression of the cell cycle and growth regulating gene, cyclin D1. (cdc.gov)
Transfection of cells with SiRNA specifically targeting eIF4E gene expression also resulted in a significant inhibition of cyclin D1 gene expression suggesting that the observed inhibition of cyclin D1 gene in the Cd-treated cells is most likely mediated through inhibition of eIF4E gene. (cdc.gov)
ADAM10 inhibition in DAPG-treated cells prevented the effects on sAPPα but only partly on intracellular and secreted Aß. (bvsalud.org)
Luteolin's positive inhibition of melanoma cell lines. (emerginginvestigators.org)
Cannabis sativa in particular cannabidiol, we propose it plays important role in helping the body fight cancer through inhibition of pain and cell growth. (biomedcentral.com)

She died without knowing that her cells would become immortal -the first to grow and survive indefinitely in culture. (crownpublishing.com)
HeLa cells are an immortal line of human cervical cancer cells used in medical research. (philosophynow.org)
The discovery of the immortal, ever-replicating HeLa cell line in 1952 has led to several breakthroughs in biomedical research for countless diseases including cancer, AIDs, and polio. (onclive.com)
Amazingly, her new tissue culture "cell line" proved to be "immortal. (candidaabrahamson.blog)
The HeLa cell line is the first immortal cell line that has been grown in laboratory conditions, allowing for massive amounts of research to be conducted over an extended period of time. (helacells.com)
The first 'immortal' human cells grown in culture, they are still alive today, though she has been dead for more than sixty years. (worldcat.org)

Henrietta's cancer cells unexpectedly did not die after a few hours - more the opposite: it was found that if nourished sufficiently they would survive and could be reproduced indefinitely. (philosophynow.org)
3 While most cancer cell samples tended to divide a few times and die before any notable research could be conducted, investigators found that Lacks' cells continued to divide indefinitely, provided they were given the right amount of nutrients. (onclive.com)

However, emerging evidence suggests that autophagy is a primary mechanism of cell death (autophagic cell death, ACD) and implicates ACD in several aspects of mammalian physiology, including tumor suppression and psychological disorders. (nature.com)
Nevertheless, the definition of autophagic cell death (ACD) has been neither universally understood nor unanimously accepted in the field 4 . (nature.com)
Moreover, glucose deprivation did not induce autophagic flux in any of the four cell lines analyzed, even though mTOR was inhibited. (gla.ac.uk)

We hypothesized that higher concentrations of vitamin C would lead to lower cell viability, as vitamin C is predicted to cause cell death in cancer cells. (csp.edu)
Our results indicated that higher concentrations of vitamin C do not necessarily correlate with lower cell viability of cervical cancer cells. (csp.edu)
This experiment analyzes the effects of luteolin on the cell viability of malignant melanoma cells using an in vitro experiment to research alternative melanoma treatments and hopefully to help further cancer research as a whole. (emerginginvestigators.org)
In previous research, the authors demonstrated that the methanol extract of A. vulgaris (AVM) has the ability to inhibit the viability of CML cells ( 10 ). (spandidos-publications.com)

Using Vero cells, we isolated a virus (NII561-2000) from a cerebrospinal fluid specimen of a 1-year-old girl with Reye syndrome. (cdc.gov)
Inoculation of a cerebrospinal fluid (CSF) specimen from the patient into Vero cells identified a virus (NII561-2000) with similar properties to HPeVs. (cdc.gov)

This study investigated the mechanism of NUPR1 in TMZ resistance in hypoxia-treated glioma cells and its mechanism in modulating autophagy. (techscience.com)
We found that hypoxia upregulated NUPR1 expression and autophagy while NUPR1 silencing suppressed hypoxia-induced TMZ resistance and autophagy in glioma cells. (techscience.com)
Autophagy is generally considered as a cell survival/protection mechanism because it removes toxic or obsolete proteins and organelles and recycles the degradation products for use as sources for energy and metabolites in anabolic pathways 3 . (nature.com)
Therefore, the relationship between autophagy and cell death remains unclear and warrants further study to harness autophagy for the treatment of various human diseases. (nature.com)
Appropriate regulation of autophagy is crucial for clearing toxic proteins from cells. (elifesciences.org)
Here, we report that the microRNA miR-1 regulates the autophagy pathway through conserved targeting of the orthologous T re-2/ B ub2/ C DC16 (TBC) Rab GTPase-activating proteins TBC-7 and TBC1D15 in Caenorhabditis elegans and mammalian cells, respectively. (elifesciences.org)
The goal of this study was to explore the regulatory mechanism of trehalose on the autophagy death of SHSY5Y cells induced by oxygen and sugar deprivation from the aspect of glycolysis. (ijpsonline.com)
On the contrary, the cell death rate and autophagy level of the experimental group were significantly lower than that of the model group. (ijpsonline.com)
Starvation of glucose, an essential nutrient, is thought to promote autophagy in mammalian cells. (gla.ac.uk)
We thus aimed to determine the role of autophagy in cell death induced by glucose deprivation. (gla.ac.uk)

1951 -- Death. (who.int)

Here, we examined the anti-lung-metastatic effects and the mechanism of lycopene in combination with sorafenib in C57BL/6 mice xenografted with Lewis lung carcinoma (LLC) cells. (frontiersin.org)
In anti-metastatic studies, lycopene has been found to inhibit the metastasis of human hepatoma SK-Hep-1 cells in athymic nude mice ( 17 ). (frontiersin.org)
In the research presented herein, the effect of SSO-induced Bcl-x splice-switching on metastatic melanoma was assessed in cell culture and B16F10 tumor xenografts. (unc.edu)
An aggressive HeLa, a metastatic ME-180 and a primary SiHa cell lines were purchased from ATCC (USA, MD). Camptothecin was supplied by Calbiochem® and cannabidiol was purchased from Sigma-Aldrich and used as a standard reference. (biomedcentral.com)

In 1966, Stanley Gartler, a cell and molecular biologist, became the first to reveal a very unpleasant truth to the cancer research community. (candidaabrahamson.blog)

In this study, exosomes released from HeLa cervical cancer cells were loaded with raloxifene to increase its bioavailability, and an aptamer was attached to the exosome membrane for targeting only HeLa cells. (techscience.com)
Lung cancer is the leading cause of cancer-related death, and NSCLC constitutes nearly 85%-90% of all cases. (scielo.br)
Lung cancer is divided into two major groups: non small cell lung cancer (NSCLC) and small cell lung cancer (SCLC). (scielo.br)
2004) Akt phosphorylation and gefitinib efficacy in patients with advanced non-small-cell lung cancer. (scielo.br)
2004) Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib. (scielo.br)
Cancer metastasis is the leading cause of death in cancer patients. (frontiersin.org)
Cancer metastasis is a complicated process, which is one of the main causes of the recurrence and death of cancer patients. (frontiersin.org)
To this topic, lycopene had been demonstrated to have synergism from the combination of oxaliplatin in a human ovarian cancer cell line ( 20 ), suggesting that the adjuvant property on the anticancer of lycopene. (frontiersin.org)
More and more test tube clinical research is being conducted, demonstrating that silver in a variety of forms is able to cause cancer cells to destroy themselves. (thesilveredge.com)
Here's a brief abstract for a test tube clinical study once again demonstrating that silver kills cancer cells. (thesilveredge.com)
In this study, a HeLa cell line was used, which is a cell line composed of human cervical cancer cells - the same kind of cancer known to be caused in women by Human Papillomavirus. (thesilveredge.com)
What's more, the researchers discovered that silver ions (i.e., ionic silver) had even greater toxic effects against the cancer cells than silver nanoparticles (i.e., metallic silver). (thesilveredge.com)
But it's certainly one more piece of evidence in a growing body of recent clinical and anecdotal evidence indicating that silver destroys a wide variety of cancer cells. (thesilveredge.com)
Also, see Another Bogus Study Claims Nanosilver Harms Human Cells for a more in-depth expose' of the ongoing drive to mis-portray silver as being harmful to human cells, when instead the studies are clearly demonstrating silver's profound anti-cancer properties. (thesilveredge.com)
The doctors who treated her took a sample of her cancer cells. (philosophynow.org)
3,4 There is, however, one pressing issue with the discovery that has negatively impacted the relationship between the medical community and with patients of color: Lacks' cervical cancer cells were collected without her or her family's consent. (onclive.com)
In honor of Black History Month, Markman looks back on the progress that has been made through the HeLa cell line, incidents that have contributed to a lack of trust in the medical community, and efforts that need to be made to ensure that the rights of underserved patient populations are maintained in clinical cancer trials. (onclive.com)
Although the collection of Lacks' cells is a subject that continues to be surrounded by controversy to this day, it's difficult to deny the progress that has been made in medical research as a byproduct of this discovery, in the field of cancer and beyond. (onclive.com)
Maddened promiscuous replication of cells is famously known as cancer. (elivieira.com)
Some genes 'detect' virus infection and cancer conspiracy, and oblige the cell to cease its activity, brake its DNA and dissolve into vesicles later 'eaten' by defense cells. (elivieira.com)
In this study, we investigated the impact of vitamin C on cervical cancer cells at varying concentrations. (csp.edu)
However, the application of SSOs against cancer targets has been hindered by poor in vivo delivery of antisense therapeutics to tumor cells. (unc.edu)
For some unknown reason, Henrietta's cancer cells continued to grow vigorously. (candidaabrahamson.blog)
Liver cells" and "monkey cells" used in cancer experiments turned out to be Henrietta's cervical cancer in disguise (see Cantwell). (candidaabrahamson.blog)
The Wall Street Journal opens its article on cell contamination with the story of cancer researcher Robert Mandic, who spent years studying a rare head-and neck cancer, and finally had his article published on the topic in Oral Oncology . (candidaabrahamson.blog)
Turns out, he learned, he had been studying nothing other than Ms. Lacks' cervical cancer cells. (candidaabrahamson.blog)
Khz, a naturally occurring compound derived from the fusion of Ganoderma lucidum and Polyporus umbellatus mycelia, inhibits the growth of cancer cells. (springer.com)
This study aimed to investigate the anti-proliferative effects of Khz on A549 lung cancer cells. (springer.com)
Zhao S, Ye G, Fu G, Cheng JX, Yang BB, Peng C. Ganoderma lucidum exerts anti-tumor effects on ovarian cancer cells and enhances their sensitivity to cisplatin. (springer.com)
The major drawback of chemotherapy regimens for treating cancer is that the cancerous cells acquire drug resistance and become impervious to further dose escalation. (emerginginvestigators.org)
Using the crude extract from the Amaranthus spinosus plant, HeLa cancer cells were assessed for cell death. (emerginginvestigators.org)
HeLa cells have enabled researchers to make incredible scientific advancements, such as in cancer research, understanding viruses, and developing new drugs. (helacells.com)
They have been used to understand how cancer cells grow, develop, and spread, as well as to develop treatments for numerous other conditions. (helacells.com)
Hela cells have been used to develop vaccines, study the effects of radiation and toxins, and research new treatments for cancer. (helacells.com)
Cancer is the leading cause of death worldwide, and there is a constant need for new treatment strategies. (lu.se)
The ratio between the IC50 values for MCF-10A and JIMT-1 cells, as a measure for the selectivity of a compound to kill cancer cells, was calculated, and found to vary between just over 1 to more than 10. (lu.se)

Nevertheless, both types of silver - ionic and metallic - worked against the cancerous cells. (thesilveredge.com)
One of the older innovations to emerge from the discovery were methods for testing for cancerous cells that proved to be so reliable, they continue to be used by investigators in the modern age. (onclive.com)

By locally oxidizing ferrocenemethanol (FcCH2OH) at a microelectrode, we probed the metabolic activity of individual HeLa cells using a scanning electrochemical microscope. (nist.gov)
The device incorporates an on-chip electrochemical cell array and a multichannel paper component, engineered to be easily aligned into a polymeric cartridge and exchanged if necessary. (csic.es)

HeLa cells, as they are called, were essential in developing the polio vaccine. (crownpublishing.com)
However, Hela cells are best known for their role in developing the polio vaccine. (helacells.com)

A prostatic stromal myofibroblast cell line (WPMY-1) was co-incubated with testosterone (1 µM) and different concentrations of capsaicin (10-100 µM) for 24 and 48 h. (techscience.com)
Here, we employ stable isotope labeling with amino acids in cell culture (SILAC)-based proteomics to quantify the response of over 6200 proteins to increasing concentrations of tunicamycin in HeLa cells. (torvergata.it)
The two cell lines were treated with various concentrations of the compounds in dose response assays, and the 50 % inhibitory concentration (IC50) was determined from dose response curves. (lu.se)

Hela cells were a crucial component of the development of the polio vaccine, as they were the first immortalized human cell line to be successfully cultured in the laboratory. (helacells.com)
In 1952, Dr. Jonas Salk and his team at the University of Pittsburgh successfully developed the polio vaccine, which was made possible by the use of Hela cells. (helacells.com)
The development of the polio vaccine with Hela cells was a ground-breaking achievement, as it allowed scientists to understand the effects of the virus on humans and provided a much-needed solution to a deadly disease that had been plaguing the world for centuries. (helacells.com)
The use of Hela cells in the development of the polio vaccine is a testament to the power of medical research and the importance of ethical considerations. (helacells.com)

Jacobson MD. Reactive oxygen species and programmed cell death. (springer.com)
The serine/threonine kinase protein kinase D1 (PKD1) is a stress-responsive kinase and sensor for reactive oxygen species, which can initiate cell survival through NF-κB signaling. (biologists.com)

HeLa cells are the most commonly used cell line in biological and medical research, particularly in cell biology and molecular biology. (helacells.com)

The MyD88 protein acts as an adapter, connecting proteins that receive signals from outside the cell to the proteins that relay signals inside the cell. (wikipedia.org)
Subsequently, these proteins activate two important transcription factors: NF-κB is a dimeric protein responsible for expression of various inflammatory cytokines, chemokines and adhesion and costimulatory molecules, which in turn triggers acute inflammation and stimulation of adaptive immunity IRFs is a group of proteins responsible for expression of type I interferons setting the so-called antiviral state of a cell. (wikipedia.org)
Moreover, we recently reported that lycopene inhibits the metastasis of human liver adenocarcinoma SK-Hep-1 cells by downregulation of the NADPH oxidase 4 (NOX4) proteins ( 18 ). (frontiersin.org)
Fusion of autophagosomes with lysosomes results in degradation of their contents and thereby removes toxic proteins and damaged organelles from cells to maintain homeostasis. (elifesciences.org)
We carry in each of our cells the chromosomes, long strands of DNA wound up into proteins. (elivieira.com)
Unexpectedly, there are few strongly downregulated proteins, despite expression of the pro-apoptotic transcription factor CHOP, suggesting that IRE1-dependent mRNA decay (RIDD) has a limited contribution to ER stress-mediated cell death in our system. (torvergata.it)
Given the role towards better design of real experiments, accumulation of oligomers of mutated proteins in AD and PD activating microglia and secreting TNFα along with other cytokines map to oxidative stress that led to cell death. (springeropen.com)

Oral squamous cell carcinoma (OSCC) is a common malignant tumor. (techscience.com)

In addition, cell death subroutines have been recently classified on the basis of mechanical and molecular aspects of cell death processes 5 . (nature.com)
However, the exact molecular action of TA in the U251 human GBM cells remains unknown. (bvsalud.org)

Even today, HeLa is the most widely used cell line in labs worldwide, bought and sold by the billions. (crownpublishing.com)
After learning about the HeLa cell line in high school, Rebecca Skloot became consumed by curiosity about the woman behind the cells. (crownpublishing.com)
Upon excitation, the molecule, which was otherwise not harmful to cells, killed the cell population of the human HeLa cell line to almost 100 percent. (chemistryviews.org)
Should the law have given control of the cell line to Henrietta's family? (philosophynow.org)
Although the research that emerged from the discovery of the HeLa cell line has helped to prevent 4.5 billion global infections and 10.3 million global deaths, the unethical and controversial nature of their discovery has raised issues with regard to privacy and consent in underrepresented patient populations. (onclive.com)
This cell line, dubbed the HeLa cell line after Lacks' name, has since become one of the most widely used human cell lines in biologic research over the past half century, with the cell lines having been used in over 100,000 scientific publications on PubMed alone. (onclive.com)
And with his propogation of her cancerous tissues he created the closest thing we have to human immortality-a cell line that never quits. (candidaabrahamson.blog)
These malignant cells became the first successful human tissue culture cell line in medical history-the now famous HeLa cell line. (candidaabrahamson.blog)
The cost, both monetary and to science, of cell line mix-ups is considerable. (candidaabrahamson.blog)
And, although some safeguards have been put into place to try to prevent such widescale contamination nowadays (in fact, HeLa's unparalleled aggressiveness is so great that if a lab wants to use it, they're not currently allowed to use any other cell line), the mess-ups continue. (candidaabrahamson.blog)
A list of cross-contaminated or misidentified cell lines," the authors write that "Cross-contamination, in which the contaminant is another cell line, was first recognized in the 1950s but, disturbingly, remains a serious issue today. (candidaabrahamson.blog)
Results demonstrated a cooperative effect between HF1 and 5FU on the drug resistant cell line, implying that administration of HF1 with 5FU results in cell death as measured by MTT assay. (emerginginvestigators.org)

It is conceivable that this change may be related to that which enables the cells of a compact tumor to become invasive. (onclive.com)
Delivery to tumor cells in vivo was achieved by encapsulating the SSO in targeted lipid nanoparticles. (unc.edu)

With this book, we too become immersed in the story of the Lacks family, and are shocked to discover that Henrietta's husband and children did not find out about her "immortality," or the enormous profits her cells had generated, until more than twenty years after her death, when scientists investigating HeLa began using blood samples from her family in research without informed consent. (crownpublishing.com)
Prior to this, scientists were unable to develop a vaccine using the traditional methods because the virus was unable to reproduce in any existing cell lines. (helacells.com)
Hela cells, however, were able to successfully replicate the virus and allowed scientists to produce a safe and effective vaccine. (helacells.com)
Her family did not learn of her 'immortality' until more than twenty years after her death, when scientists began using her husband and children in research without informed consent. (worldcat.org)

Extracts inhibited oxidative stress and reduced activity of key enzymes involved in the pathogenesis of both diseases in neuroblastoma cells. (emerginginvestigators.org)

The 2 cell types possess volumes, nuclear cross-sections, plating efficiencies, and generation times which are identical within experimental error, but display widely different cross-sectional areas, suggesting that the basic change occurs in the cell surface," wrote the authors of the study. (onclive.com)
Since their discovery, Hela cells have been widely used in medical research as a tool to better understand a variety of human diseases and conditions. (helacells.com)

Metz H, Busch SE, Hanke ML, Kargl J, Kim KH and Houghton M (2014) Insulin receptor substrate-1 regulates immune cell content in lung adenocarcinoma. (scielo.br)

Lacks' family was unaware that her cells, now known worldwide as HeLa cells, were being used for research until more than 20 years after her death. (jhu.edu)
So years passed, and the Lacks family remained completely unaware that these cells existed. (philosophynow.org)
You may recall from last post that Ms. Lacks' cells, entitled HeLa, were taken without her permission, and without recompense to the family. (candidaabrahamson.blog)
Gey, somewhat stingy in offering the Lacks family anything, was extremely generous in sharing the HeLa cells. (candidaabrahamson.blog)

Based on these findings, investigators concluded that these methods would be useful for examining mammalian cell growth, physiology, genetics, and biochemistry in addition to examining how cells respond to drugs, viruses, and high-energy radiation. (onclive.com)

Further, we found that the cytokine interferon-β (IFN-β) can induce miR-1 expression in mammalian cells, reducing TBC1D15 levels, and safeguarding against proteotoxic challenges. (elifesciences.org)

If you could pile all HeLa cells ever grown onto a scale, they would weigh more than fifty million metric tons-more than a hundred Empire State Buildings. (crownpublishing.com)

For some cells, FcCH2OH+ penetrated the cell membrane and was reduced, leading to an enhanced current known as positive feedback. (nist.gov)
For other cells, FcCH2OH diffusion was blocked by the cell membrane, leading to a lower current known as negative feedback. (nist.gov)

The subsequent chapter discusses the effect of the mechanism of cell death of neutrophil granulocytes on the realization of the inflammatory process. (novapublishers.com)

Capsaicin (10-100 µM) significantly inhibited testosterone-treated WPMY-1 cell growth at 48 h by MTT assay. (techscience.com)
In THP1 monocytic leukemia cells 1,25(OH)_{2} D_{3} stimulated TrxR mRNA levels 2-4‐fold by 4-8 h and enhanced TrxR activity (60%) (as measured by the dithionitrobenzole‐assay) after 24 h, which declined below baseline after 96 h. (iospress.com)

Sustaining an appropriate level of genes encoding DNA repair factors is thought to be necessary for cell survival by preventing the accumulation of DNA mismatches and epigenetic alterations. (novapublishers.com)
Steen and Collas 2001 (this issue) now provide some tantalizing data that links nuclear lamina organization to cell survival. (rupress.org)
If genes are affected, further replication and survival of the cells becomes jeopardized (what can be observed in elders, e. g., in scarceness of their defense cells). (elivieira.com)

Biocompatibility of composites was tested on HELF and HeLa cells and revealed no toxic effects. (nature.com)

This, in my opinion, could ultimately have profound implications for women diagnosed with abnormal cell growth in the cervix caused by Human Papillomavirus. (thesilveredge.com)
From groundbreaking scientific research to the production of products and treatments, HeLa cells have made a profound impact on our lives. (helacells.com)

B) Stability of cyaninplatin in complete RPMI 1640 culture medium containing 10% fetal bovine serum (FBS) at 37°C. (C) Stability of cyaninplatin in the lysate of A2780 cells at 37°C. (D) Reduction of cyaninplatin under sono-activation (FUS: 1.75 MHz, 4 W, 0 to 60 min) in phosphate-buffered saline (PBS) containing 5 mM ascorbate at 37°C with (E) corresponding reduction profile of cyaninplatin with or without FUS. (worldmedicinefoundation.com)
Forty different human cell culture lines, used extensively in labs worldwide, were contaminated with HeLa. (candidaabrahamson.blog)
In 1974, a cell culture expert by the name of Walter Nelson-Rees discovered widespread contamination of cell lines with HeLa, and had the moxie to share his findings-publicly. (candidaabrahamson.blog)

Second , HeLa cells have been used to create a wide variety of products, from vaccines and insulin to synthetic skin. (helacells.com)

Different strains of HeLa cells, all descended from that original sample, have been invaluable in a wide range of medical research ever since. (philosophynow.org)
In addition, HeLa cells have been used in tissue engineering and organ transplantation, making them invaluable for medical advancement. (helacells.com)
The unique properties of Hela cells allow them to divide rapidly and continuously in the laboratory and make them invaluable in medical research. (helacells.com)

Exposure of human cell lines HCT15, PLC/PR/5, HeLa and Chang, to cadmium chloride (Cd) resulted in a dose-dependent toxicity and death. (cdc.gov)
The nuclear lamina is a thin (20 nm) yet insoluble protein meshwork that, in higher cells, lines the nucleoplasmic face of the nuclear envelope (NE). (rupress.org)
These cell lines were cultured in Eagle minimum essential medium with 6 mmol/L L-glutamine, 1.1 g/L sodium bicarbonate, antimicrobial agents (0.2 g/L gentamicin, 0.25 g/L amphotericin B), and 8% fetal bovine serum at 37°C under 5% carbon dioxide. (cdc.gov)
After locating a gene specific to African-Americans in 18 separate supposedly Caucasian cell lines, Gartler came to the conclusion that HeLa was overtaking these cells. (candidaabrahamson.blog)
In that same year, 5 cell lines claimed to be of human lineage were sent here from the Soviet Union. (candidaabrahamson.blog)
All of the lines were discovered to be of HeLa origin (see Lucey & Rees). (candidaabrahamson.blog)
Hundreds of scientific reports based on fraudulent cell lines were published, and tainted research, estimated in value well in excess of 10 million dollars , was discredited. (candidaabrahamson.blog)

Flow cytometry analysis showed that the percentage of A549 cells in sub-G1 phase of the cell cycle increased in response to Khz treatment. (springer.com)

When cells replicate in mitosis (the rate of replication differs from tissue to tissue - skin and gut cells renew every week or so, while neurons may last a lifetime), the DNA must be copied and inherited by daughter cells. (elivieira.com)

HeLa cells have done some truly remarkable things in the past few decades. (helacells.com)

In one study, the authors focus on the effects of aging on adult neurogenesis, a process of producing new neurons from neural stem cells and neural progenitor cells in the neocortex, comparing the dentate gyrus and subventricular zone. (novapublishers.com)
This could lead to the prolonged activation of microglia that result in death of surrounding neurons. (springeropen.com)
Parkinson's disease (PD) is the second most prevalent neurodegenerative disorder that causes death of dopaminergic neurons in substantia nigra pars compacta of the midbrain, which leads to the decline in the synthesis of dopamine (Mhyre et al. (springeropen.com)

The recovery of blood-forming stem cells, determined as endogenous spleen colonies, was markedly stimulated by priming irradiation. (go.jp)
But mice pretreated at this priming dose did not show a marked recovery of peripheral blood cell counts after challenging irradiation with mid- or sublethal X-ray doses (a significant, albeit slight, increase in the blood cell counts in the preirradiated group was observed after a lower challenging exposure to 5.0 Gy). (go.jp)
The current version of the model gives predictions of the yields and sizes of translocations, dicentrics, rings, and more complex-type aberrations formed in the G 0 /G 1 cell cycle phase during the first cell division after irradiation. (bioone.org)
The material has the potential to selectively kill cells upon irradiation. (chemistryviews.org)
The new structure was biocompatible, but upon irradiation it killed its host cell. (chemistryviews.org)

Results of Western blot analysis and immunoprecipitation experiments demonstrated a significant induction of ubiquitination of eIF4E in the Cd treated cells. (cdc.gov)
5 Two methods of quick and simple plating of single HeLa cells that results in the growth and formation of colonies from individual cells were detailed in a paper that was published in 1956. (onclive.com)

New Research on Cell Aging and Death reviews previous literature to describe the main behavioral and biochemical characteristics of the SAMP8 mouse model, discussing its main advantages as well as potential weaknesses to model age-related diseases. (novapublishers.com)
Showing posts with label Hela cells and research . (helacells.com)
First , HeLa cells have been used in ground-breaking scientific research. (helacells.com)
HeLa cells have also been used in drug screening, genetic engineering, and cloning research. (helacells.com)
Despite the immense benefit of Hela cells to medical research, it is essential to recognize the potential ethical implications of their use and to strive to ensure that consent and proper ethical protocols are always followed when utilizing human cells in research. (helacells.com)
Hela cells and organ transplants have been integral to the advancement of medical research and treatment. (helacells.com)

A priming X-ray exposure to 0.05-0.10 Gy or 0.3-0.5 Gy imparts radioresistance (decrease in bone marrow death rate after high-dose X-rays) in mice 2-2.5 months or 9-17 days postexposure, respectively. (go.jp)
The reduction in bone marrow death seems chiefly due to the stimulated recovery of blood-forming stem cells. (go.jp)
Among immunodeficient individuals, toxoplasmosis most often occurs in those with defects of T-cell-mediated immunity, such as those with hematologic malignancies, bone marrow and solid organ transplants, or acquired immunodeficiency syndrome ( AIDS ).In most immunocompetent individuals, primary or chronic (latent) T gondii infection is asymptomatic. (medscape.com)

The human ortholog MYD88 seems to function similarly to mice, since the immunological phenotype of human cells deficient in MYD88 is similar to cells from MyD88 deficient mice. (wikipedia.org)
We present a computational model for calculating the yield of radiation-induced chromosomal aberrations in human cells based on a stochastic Monte Carlo approach and calibrated using the relative frequencies and distributions of chromosomal aberrations reported in the literature. (bioone.org)
As usual, the authors use the study to claim silver is harmful to human cells in general, which of course is a crock, as silver has been repeatedly demonstrated to protect normal, healthy human cells. (thesilveredge.com)
The remarkably resilient cells became the first human cells to be cultured continuously for experiments. (philosophynow.org)

Then, these cells were inoculated with a CSF specimen (100 µL per well) from a patient with a diagnosis of Reye syndrome and cultured at 33°C for 2 weeks. (cdc.gov)

When HeLa cells were first cultured, no one really knew the potential they had, not even the researchers. (philosophynow.org)

Mol Cell Proteomics 8:519-534. (scielo.br)

Programmed cell death (PCD), as described by Lockshin and Williams 14 , is defined as controlled cell death evoked by intracellular systems. (nature.com)

PCD has fundamental functions in tissue development and homeostasis, as PCD is activated to sculpt or remove structures, regulate cell numbers, and eliminate unnecessary or dysfunctional cells. (nature.com)
The metastasizing cells penetrate the blood vessel wall by invasiveness, and migrate in the circulation, extravasate out of the blood vessels, colonize in the distant tissue, initiate angiogenesis, and finally grow at the new site ( 2 , 3 ). (frontiersin.org)
This means stem cells (which give birth to themselves and to tissue-function-specialized cells all over the body) diverge from the ancestral type, and this ancestral type is the fertilized egg from our mothers. (elivieira.com)

In the early stage of ischemia and hypoxia, cells will increase the energy supply to the ischemic area, especially the functional area of the ischemic penumbra, by regulating glycolysis. (ijpsonline.com)

In the concluding review the authors evaluated the effects of natural and chemical compounds on promoter activities of several human DNA repair-associated genes in HeLa S3 cells. (novapublishers.com)

Anatomy29

Organisms13

Diseases7

Chemicals and Drugs112

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Psychiatry and Psychology1

Phenomena and Processes67

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

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Health Care6

Henrietta Lacks5

Proliferation11

Apoptosis18

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Cervical cancer cell3

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Immortal6

Indefinitely2

Autophagic3

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20002

Autophagy10

19511

Metastatic4

19661

Cancer36

Cancerous cells2

Electrochemical2

Developing the polio v2

Concentrations3

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Biology1

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Molecular2

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Scientists4

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Widely2

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Lacks4

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Mammalian cells1

Grown1

Membrane2

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Assay2

Survival3

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Profound2

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Invaluable3

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Results2

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