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Cell CycleCell Cycle ProteinsMitosisG1 PhaseS PhaseCyclinsMeiosisG2 PhaseCDC2 Protein KinaseCell DivisionCell Cycle CheckpointsDisease ProgressionCyclin BProtein-Serine-Threonine KinasesCell ProliferationRetinoblastoma ProteinCyclin-Dependent Kinase Inhibitor p27Cyclin-Dependent KinasesPhosphorylationSaccharomyces cerevisiaeDNA ReplicationApoptosisCyclin-Dependent Kinase Inhibitor p21Cyclin-Dependent Kinase 2Molecular Sequence DataCyclin D1Cell Line, TumorDNA-Binding ProteinsMutationCyclin ENuclear ProteinsSignal TransductionSaccharomyces cerevisiae ProteinsInterphaseSpindle ApparatusCell NucleusCyclin ASchizosaccharomycesTranscription FactorsSchizosaccharomyces pombe ProteinsCell LineG0 PhaseFungal ProteinsGenes, FungalCyclin-Dependent Kinase 4Protein KinasesHeLa CellsE2F Transcription FactorsTumor Suppressor Protein p53G2 Phase Cell Cycle CheckpointsCDC2-CDC28 KinasesAmino Acid SequenceRNA, MessengerUbiquitin-Protein Ligase ComplexesTranscription, GeneticDNA DamageTime FactorsCyclin B1Flow CytometryTumor Suppressor ProteinsAnaphase-Promoting Complex-Cyclosomecdc25 PhosphatasesE2F1 Transcription FactorBase SequenceGenes, cdcCells, CulturedModels, BiologicalStarfishTumor Cells, CulturedGene Expression Regulation, FungalG1 Phase Cell Cycle CheckpointsCyclin DGene Expression RegulationOocytesBlotting, WesternMenstrual CycleCell SurvivalPhenotypeNocodazoleCentrosomeXenopus ProteinsDown-RegulationBromodeoxyuridineRNA, Small InterferingProtein BindingDNATranscription Factor DP1TransfectionGene Expression Regulation, NeoplasticMicrotubule-Associated ProteinsCell DifferentiationProto-Oncogene ProteinsFibroblastsRNA InterferenceRetinoblastoma-Binding Protein 1FertilizationHistonesZygoteAntineoplastic AgentsXenopus
ApoptosisSpindleArrestKinasesBasalRegulatesAltersCentriolesMEK1RolesVertebrate cellsCytokinesisCentrosomeInterphaseTransitionPathwaysCiliaMicrotubuleHeLaStructures calledRegulationBiologyProteinInhibitionDuplicationImportantNucleusMalignantFormationChromosomesAffectsAvoidDaughterColonMachineryFigureFunctionsPlaysSingleDeathEffectReportsContentGrowthAnalysisDamageMake
Apoptosis10
  • The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, 5-ethynyl-2′-deoxyuridine assay, propidium iodide (PI) staining, annexin V-fluorescein isothiocyanate/PI staining, and transwell assay were employed to test the proliferation, apoptosis, migration ability, and invasiveness of COAD cells. (hindawi.com)
  • Upregulating the level of miR-323a-3p impaired the proliferation, migration, and invasion of COAD cells and promoted apoptosis, whereas supplementing NEK6 alleviated the damage of the proliferation, migration, and invasion of COAD cells caused by miR-323a-3p and inhibited miR-323a-3p-induced apoptosis. (hindawi.com)
  • These findings indicate that miR-323a-3p regulates the proliferation, migration, invasion, and apoptosis of COAD cells by targeting NEK6. (hindawi.com)
  • Non-receptor tyrosine kinase involved in various processes such as cell cycle progression, apoptosis, mitotic recombination, genetic instability and histone modifications. (caslab.com)
  • Here, we examined the role of the Bcl-2 family proteins in AURK inhibition-induced apoptosis in colon cancer cells. (bvsalud.org)
  • We found that alisertib and danusertib, two small-molecule inhibitors of AURK, are inefficient inducers of apoptosis in HCT116 and DLD-1 colon cancer cells, the survival of which requires at least one of the two antiapoptotic Bcl-2 family proteins, Bcl-xL and Mcl-1. (bvsalud.org)
  • We further identified Bcl-xL as a major suppressor of alisertib- or danusertib-induced apoptosis in HCT116 cells. (bvsalud.org)
  • Together, these results define the Bcl-2 protein network critically involved in AURK inhibitor-induced apoptosis and suggest that BH3-mimetics targeting Bcl-xL may help overcome resistance to AURK inhibitors in cancer cells. (bvsalud.org)
  • Additionally, miR-1291 induced cell apoptosis. (bvsalud.org)
  • Prolonged mitotic arrest triggers partial activation of apoptosis, resulting in DNA damage and p53 induction. (mdc-berlin.de)
Spindle6
  • The activity of NEK6 plays important roles in mitotic spindle kinetochore fiber formation, metaphase-anaphase transition, cytokinesis, and checkpoint regulation [ 3 , 4 ]. (hindawi.com)
  • Centrioles play critical roles in organizing the assembly of the mitotic spindle and templating the formation of primary cilia. (elifesciences.org)
  • Centrosomes nucleate the formation of the microtubule cytoskeleton in interphase cells and form the poles of the mitotic spindle during cell division. (elifesciences.org)
  • At the onset of mitosis, centrosomes become the core structures of spindle poles and direct the formation of mitotic spindles. (biomedcentral.com)
  • In early mitosis, the cohesion between the two pairs of centrioles is broken and each of them participates in the formation of the mitotic spindle pole. (biomedcentral.com)
  • During mitosis the two centrosomes are present and form the mitotic spindle poles. (biomedcentral.com)
Arrest2
  • However, impaired splicing affects only a subset of human transcripts, enriched for mitotic cell cycle factors, leading to mitotic arrest. (biomedcentral.com)
  • Knockdown of SRSF2, its splicing targets SLMAP-L, or CETN3-S caused colon cancer cells to arrest in G1 phase of the cell cycle. (bvsalud.org)
Kinases1
  • Aurora kinases (AURKs) are mitotic kinases important for regulating cell cycle progression. (bvsalud.org)
Basal2
  • In quiescent cells, centrioles dock at the plasma membrane and act as basal bodies that template the formation of cilia and flagella ( Breslow and Holland, 2019 ). (elifesciences.org)
  • Basal dynamics of p53 reveal transcriptionally attenuated pulses in cycling cells. (mdc-berlin.de)
Regulates1
  • However, a major unresolved question is how each cell regulates the precise number of its motile cilia during differentiation. (elifesciences.org)
Alters2
  • Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. (genetex.com)
  • In addition HIV-1 Vpr protein alters the cell-cycle regulation by hijacking centrosome functions. (biomedcentral.com)
Centrioles7
  • Animal cells use structures called centrioles to help them divide their sets of chromosomes accurately. (elifesciences.org)
  • When cells are about to divide, they make a new set of centrioles by assembling a variety of proteins. (elifesciences.org)
  • if too many or too few centrioles are built, cell division errors can occur that lead to the generation of new cells with abnormal numbers of chromosomes. (elifesciences.org)
  • The modification of STIL at a specific location by PLK4 was needed to link it to another protein in the outer wall of the centriole, and was also necessary for the cells to build new centrioles. (elifesciences.org)
  • Cells in which PLK4 was unable to modify STIL had too few centrioles when they were beginning to divide. (elifesciences.org)
  • Airway progenitor cells contain two parental centrioles (PC) and form structures called deuterosomes that nucleate centrioles during amplification. (elifesciences.org)
  • In G1 phase, cells have a single centrosome consisting of two centrioles joined by cohesion fibers. (biomedcentral.com)
MEK11
  • Our group has previously demonstrated the involvement of the alternatively spliced variants ERK1c and MEK1b (ERK1 is also known as MAPK3, and MEK1 as MAP2K1) in mitotic Golgi fragmentation. (biologists.com)
Roles1
  • They localize in close proximity to the nucleus for the duration of interphase and play major roles in numerous cell functions. (biomedcentral.com)
Vertebrate cells1
  • Centrosomes are the major microtubule organizing structures in vertebrate cells. (biomedcentral.com)
Cytokinesis2
Centrosome1
  • Centrosome duplication is tightly regulated and limited at once per cell cycle through a mechanism that prevents re-duplication. (biomedcentral.com)
Interphase1
Transition1
  • This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. (genetex.com)
Pathways2
  • miRNAs expressed in a wide variety of human cancers can regulate posttranscriptional gene expression by binding to the 3′ untranslated region of the target mRNAs and act as oncogenes or tumor suppressors to regulate cell signaling pathways, affecting tumorigenesis and tumor progression [ 17 ]. (hindawi.com)
  • A number of direct and indirect radiation interaction pathways can produce damage to the DNA of irradiated cells. (cdc.gov)
Cilia4
  • Multiciliated cells (MCC) contain hundreds of motile cilia used to propel fluid over their surface. (elifesciences.org)
  • Our results demonstrate that a cell-intrinsic surface area-dependent mechanism controls centriole and cilia abundance in multiciliated cells. (elifesciences.org)
  • Each MCC contains hundreds of motile cilia, microtubule-based organelles that generate the motive force to move fluid over the surface of the cell. (elifesciences.org)
  • suggesting that establishment of the correct number of cilia per cell is important for function. (elifesciences.org)
Microtubule1
  • In animal cells, centrosomes represent the major microtubule-organizing structures (MTOC). (biomedcentral.com)
HeLa1
Structures called1
  • Specialized cell structures called ribosomes are the cellular organelles that actually synthesize the proteins (RNA transcription). (cdc.gov)
Regulation1
Biology2
  • Finzel, A. and Grybowski, A. and Strasen, J. and Cristiano, E. and Loewer, A. Molecular Biology of the Cell 27 (15): 2360-2367. (mdc-berlin.de)
  • Molecular Biology of the Cell 23 (4): 567-576. (mdc-berlin.de)
Protein5
  • The mitotic reorganization of the Golgi is accompanied by a temporary block in Golgi functioning, as protein transport in and out of the Golgi stops. (biologists.com)
  • By performing cell biological and biochemical experiments using human cells, Moyer and Holland show that PLK4 interacts with a protein called STIL that is found in the central part of the centriole. (elifesciences.org)
  • The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. (genetex.com)
  • miR-1291 also suppressed DCLK1 mRNA and protein expression in HCT116 cells that expressed DCLK1. (bvsalud.org)
  • Cell-type-specific processing of the amyloid precursor protein by Presenilin during Drosophila development. (mdc-berlin.de)
Inhibition2
  • Rescue of SLMAP-L or CETN3-S splice isoform in SRSF2 knockdown colon cancer cells could effectively reverse the inhibition of cell proliferation by SRSF2 knockdown through mediating cell cycle progression. (bvsalud.org)
  • Hyper-activation of ATM upon DNA-PKcs inhibition modulates p53 dynamics and cell fate in response to DNA damage. (mdc-berlin.de)
Duplication1
Important1
Nucleus1
  • Each cell (except for red blood cells) contains a nucleus that houses these chromosomes. (cdc.gov)
Malignant1
  • Knockdown of SLMAP-L or CETN3-S splice isoform could suppress the growth of colon cancer cells, predicting their role in malignant proliferation of colon cancer cells. (bvsalud.org)
Formation1
  • It was identified that miR-1291 significantly suppressed the proliferation, invasion, cell mobility and colony formation of CRC cells. (bvsalud.org)
Chromosomes2
Affects1
  • Cellular conference call: external feedback affects cell-fate decisions. (mdc-berlin.de)
Avoid2
  • Store at -20°C. Avoid freeze / thaw cycles. (caslab.com)
  • Avoid multiple freeze-thaw cycles. (genetex.com)
Daughter1
  • Golgi fragmentation is a highly regulated process that allows division of the Golgi complex between the two daughter cells. (biologists.com)
Colon2
  • The activity of NEK6 is enhanced in several cancer cells, including colon adenocarcinoma (COAD) cells. (hindawi.com)
  • Both in vitro and in vivo, SRSF2 significantly accelerated the proliferation of colon cancer cells. (bvsalud.org)
Machinery1
  • The results presented by Moyer and Holland help us to understand how dividing cells build the complex machinery that enables them to pass on their genetic material accurately. (elifesciences.org)
Figure1
  • The number of centrosomes within a cell is strictly controlled [ 5 ] (Figure 1 ). (biomedcentral.com)
Functions1
  • Radiation can disrupt the structure of the DNA (and other macromolecules), thereby disrupting normal cell and organ functions. (cdc.gov)
Plays1
  • Testing the activity of PLK4 in fruit flies revealed that it plays a similar role as in human cells. (elifesciences.org)
Single2
  • The p53 response in single cells is linearly correlated to the number of DNA breaks without a distinct threshold. (mdc-berlin.de)
  • Disentangling pro-mitotic signaling during cell cycle progression using time-resolved single-cell imaging. (mdc-berlin.de)
Death1
  • however, the utility of these inhibitors as inducers of cancer cell death has thus far been limited. (bvsalud.org)
Effect1
  • In the present study, the expression and effect of miR-1291 in CRC cells was investigated. (bvsalud.org)
Reports2
  • Benary, M. and Bohn, S. and Lüthen, M. and Nolis, I.K. and Blüthgen, N. and Loewer, A. Cell Reports 31 (2): 107514. (mdc-berlin.de)
  • Sheng, C. and Mendler, I.H. and Rieke, S. and Snyder, P. and Jentsch, M. and Friedrich, D. and Drossel, B. and Loewer, A. Cell Reports 27 (1): 48-58. (mdc-berlin.de)
Content1
  • The total chromosomal content of a cell involves approximately 105 genes in a specialized macromolecule of deoxyribonucleic acid (DNA). (cdc.gov)
Growth1
  • When the body needs to make more cells, for example during growth or repair, existing cells divide in two in order to replicate themselves. (elifesciences.org)
Analysis1
  • IHC-P analysis of human mantle cell lymphoma tissue using GTX33611 Cyclin D1 antibody [RM241]. (genetex.com)
Damage1
Make1
  • Cells depend on their DNA for coding information to make various classes of proteins that include enzymes, certain hormones, transport proteins, and structural proteins that support life. (cdc.gov)