Cell cycle regulators expression. Medical search. Frequent questions

Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
In HL-60 leukaemia cells, di-GA activated caspase 3 and dose-dependently induced apoptosis. (nature.com)
These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
Tumour cells acquire the ability to proliferate uncontrollably, resist apoptosis, sustain angiogenesis and evade immune surveillance. (researchgate.net)
Activated p53 transcriptionally regulates hundreds of genes that are involved in multiple biological processes, including in DNA damage repair, cell cycle arrest, apoptosis and senescence. (nih.gov)
In this Review, we discuss the multiple molecular mechanisms of p53 regulation and how they modulate the induction of apoptosis or cell cycle arrest following DNA damage. (nih.gov)
As a consequence, any modulations of the target transcript expression, miRNAs can affect various signalling pathways and cellular process such as apoptosis, proliferation or differentiation. (ukessays.com)
Cancer genetics, DNA damage response, apoptosis, cell cycle My current research is focused on two transcription factors that respond to DNA damage and play important roles in regulating tumor development. (mdanderson.org)
The first project focuses on E2F1, a regulator of genes important for cell cycle progression and apoptosis. (mdanderson.org)
Activating transcription factor-(ATF-) 3, a stress-inducible transcription factor, is rapidly upregulated under various stress conditions and plays an important role in inducing cancer cell apoptosis. (hindawi.com)
First, we found that GS-002 significantly inhibited cell proliferation and induced cell apoptosis in dose-dependent manners. (hindawi.com)
Furthermore, we found that GS-002 induced more cell apoptosis in ATF-3-overexpressing cells. (hindawi.com)
This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. (cancerindex.org)
Low levels of pyruvate induced by a positive feedback loop protects cholangiocarcinoma cells from apoptosis. (cancerindex.org)
BRCA1 expression mediates apoptosis, cell cycle arrest and decreased viability in response to doxorubicin treatment. (conicet.gov.ar)
Western blot analysis was performed to study Boswellia sacra essential oil-regulated proteins involved in apoptosis, signaling pathways, and cell cycle regulation. (biomedcentral.com)
Boswellia sacra essential oil hydrodistilled at 100 o C was more potent than the essential oil prepared at 78 o C in inducing cancer cell death, preventing the cellular network formation (MDA-MB-231) cells on Matrigel, causing the breakdown of multicellular tumor spheroids (T47D cells), and regulating molecules involved in apoptosis, signal transduction, and cell cycle progression. (biomedcentral.com)
These abnormalities result in significant alterations in gene expression related to cell proliferation, apoptosis, migration and immune signaling pathways. (spandidos-publications.com)
Flow cytometry was used to examine apoptosis, cell cycle distribution, and accumulation of reactive oxygen species (ROS). (jcancer.org)
Moreover, it induced cell cycle arrest in the G0/G1 phase, apoptosis, and ROS generation in these cells. (jcancer.org)
Silibinin considerably reduced the development of oral cancer cells by inducing apoptosis, G 0 /G 1 arrest, ROS generation, and activation of the JNK/c-Jun pathway. (jcancer.org)
Primarily, p53 protects the cell from malignant transformation through cell cycle arrest and apoptosis. (uib.no)

It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
Reducing CDK5 activity in human MTC cells down-regulated these cell cycle regulators suggesting that CDK5 activity is critical for cell cycle progression and MTC proliferation. (oncotarget.com)
This gene is thought to regulate cell cycle progression. (wikipedia.org)
In different assays and cell types, overexpression of this protein has been shown to activate or suppress cell cycle progression. (wikipedia.org)
Some studies showed that blocking cell cycle progression ameliorated fibrosis. (nature.com)
Mitotic chromosomal instability - the inability to faithfully segregate equal chromosome complements to two daughter cells during mitosis - is a widespread phenomenon in solid tumours that is thought to serve as the fuel for tumorigenic progression. (researchgate.net)
This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. (cancerindex.org)
T he presence and activity of various proteins are required in the cell cycle progression. (ukessays.com)
As a result, it negatively regulates cyclin-D-dependent phosphorylation of the retinoblastoma (pRb) gene product, thus blocking cell-cycle progression from G 1 -to S-phase ( 2 - 4 ). (iiarjournals.org)
Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. (genetex.com)
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. (cancerindex.org)
Finally, GADD153 depletion significantly abrogates BRCA1 influence on cell cycle progression and cell death in response to doxorubicin treatment. (conicet.gov.ar)
During cell growth and proliferation, ubiquitin plays an outsized role in promoting progression through the cell cycle. (intechopen.com)
Progression through the cell cycle is driven by the oscillating activity of Cyclin Dependent Kinases (CDKs). (intechopen.com)

Enables cyclin-dependent protein serine/threonine kinase regulator activity. (nih.gov)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
The induction of ATF-3 expression was mediated by mitogen-activated protein kinase (MAPK) signaling pathways in GS-002-treated cells. (hindawi.com)
In this review, we summarize the basic principles manipulating the abovementioned epigenetic pathways and highlight the evidence of the promising clinical and preclinical results using small-molecule inhibitors against chromatin regulators for cancer treatment. (frontiersin.org)
Numerous E3 ubiquitin ligases, which facilitate the ubiquitination of specific substrates, have been shown to control G1/S. In this chapter, we will discuss components in the ubiquitin proteasome system that are implicated in G1/S control, how these enzymes are interconnected, gaps in our current knowledge, and the potential role of these pathways in the cancer cycle and disease proliferation. (intechopen.com)
The mechanisms of radioresistance are still poorly understood, despite it has been suggested that miRNAs play an important role in cell signaling pathways. (frontiersin.org)
Indeed, it has been shown that miRNAs play an important role in gene expression, mainly when associated with the monitoring of several cell and metabolic pathways, being also an essential component of the gene silencing machinery in most eukaryotic organisms ( 4 , 8 ). (frontiersin.org)
Consistently, the essential oil represses signaling pathways and cell cycle regulators that have been proposed as therapeutic targets for breast cancer. (biomedcentral.com)
Cellular proteostasis involves the coordinated and compensatory action of pathways that control biogenesis, folding, trafficking and breakdown of proteins allowing the cell to adapt to physiological or pathological environmental changes. (elifesciences.org)

Deletions of multiple GID subunits compromise cell proliferation, and this defect is accompanied by deregulation of critical cell cycle markers such as the retinoblastoma (Rb) tumor suppressor, phospho-Histone H3 and Cyclin A. We identify the negative regulator of pro-proliferative genes Hbp1 as a bonafide GID/CTLH proteolytic substrate. (elifesciences.org)
While mutations in p53 occur in less than 10% of acute myeloid leukaemia (AML), over-expression of the main negative regulator of p53, MDM2, is frequently observed, representing a mechanism of p53 silencing. (uib.no)

CDKN1C , or cyclin-dependent kinase inhibitor 1C, is a gene that encodes a protein implicated in cell cycle regulation. (medscape.com)
They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. (novusbio.com)
1) The regulation of these protein levels is vital for the understanding of the cell cycle control and its dysregulation. (ukessays.com)
1) For example, the down-regulation of miR15s and miR - 16 - 1 in chronic lymphocytic leukaemia, prostate cancer and pituitary tumours, leading to the inhibition of tumour growth and induce cell cycle arrest at the G 1 - phase by target cell cycle regulators ( cyclin D1, cyclin E1, cyclin D3 and CDK6). (ukessays.com)
De-regulation of cell-cycle control, in particular G 1 - to S-phase transition, is implicated in the pathogenesis of most types of human cancer, including ovarian cancer ( 1 ). (iiarjournals.org)
As main findings, 23 miRNAs were already identified as being involved in genetic regulation of PCa cell response to RT. (frontiersin.org)
role in cell cycle regulation. (atlasgeneticsoncology.org)
The complex life cycle of Plasmodium falciparum requires coordinated gene expression regulation to allow host cell invasion, transmission, and immune evasion. (biorxiv.org)
Furthermore, post-transcriptional gene expression regulation by cellular and/or EBV-derived microRNAs has attracted considerable attention. (spandidos-publications.com)
Regulation of the timing of cell differentiation and cell fate choice are key issues for making organs of the right size, shape and cell composition. (biologists.com)
Despite their importance for immunity against sexually transmitted infections (STIs), the composition of female reproductive tract (FRT) memory T cell populations in response to changes within the local tissue environment under the regulation of the menstrual cycle remains poorly defined. (cdc.gov)
INTERPRETATION: These data demonstrate that periodic shifts in the immune landscape under menstrual cycle regulation drives bystander CCR5+ CD4 T cell recruitment and HIV susceptibility in the female reproductive tract. (cdc.gov)

We find that downstream target genes of the tumor suppressor, retinoblastoma protein, including genes encoding cell cycle regulators such as CDKs, cyclins and CDK inhibitors, are significantly upregulated in malignant mouse tumors in a CDK5-dependent manner. (oncotarget.com)
In this study, we explored the role of Plk1 in the classical kidney fibrosis model, UUO, and cultured fibroblast and renal tubular epithelial cells by using specific Plk1 inhibitors, genetic silencing, and heterozygous global knockout mice. (nature.com)
The CDKN2A/B locus contains genes encoding cell cycle inhibitors, including p16 Ink4a , which have not yet been implicated in the control of hepatic glucose homeostasis. (diabetesjournals.org)

Dmp1 is a unique tumor suppressor that activates Arf expression and thus induces p53-dependent cell cycle arrest. (eurekalert.org)
This finding is consistent with the fact that many FOXC2-regulated genes are involved in cell cycle arrest. (elifesciences.org)
We also assessed the effect of WSC on the expression of genes involved in cell cycle arrest and inflammation. (who.int)
However, WSC induced cell cycle arrest and cellular senescence mediated by the p53-p21 pathway. (who.int)

Specifically, we discuss how the interaction of p53 with DNA and chromatin affects gene expression, and how p53 post-translational modifications, its temporal expression dynamics and its interactions with chromatin regulators and transcription factors influence cell fate. (nih.gov)

Complex patterns of posttranslational modifications of chromatin including acetylation of histones establish combinatorial signals that are apparently interpreted by chromatin-binding proteins and the gene expression machinery to control transcriptional activity of individual genes. (aacrjournals.org)
Although BRCA1 interaction with key proteins involved in DNA repair is well known, its role as a co-regulator in the transcriptional response to DNA damage remains poorly understood. (conicet.gov.ar)
A focused survey of BRCA1 regulated genes in prostate carcinoma reveals that multiple regulators of genome stability and cell cycle control, including: BLM, FEN1, DDB2, H3F3B, BRCA2, CCNB2, MAD2L1 and GADD153, are direct transcriptional targets of BRCA1. (conicet.gov.ar)
These findings define a novel transcriptional pathway through which BRCA1 orchestrates cell fate decisions in response to genotoxic insults, and suggest that BRCA1 status should be considered for new chemotherapeutic treatment strategies in prostate cancer. (conicet.gov.ar)
Also our study indicated that WSC induced an increase in the transcriptional expression of matrix metalloproteinases, MMP-2 and MMP-9 and an immune response regulator, Toll Like Receptor-4. (who.int)

Positive regulators include two protein groups that allow cells to pass through regulatory checkpoints: cyclins and cyclin-dependent kinases (CDKs). (jove.com)
Cyclins can be categorized as G 1 , G 1 /S, S, or M cyclins based on the cell cycle phase or transition they are most involved in. (jove.com)
Generally, levels of a given cyclin are low during most of the cell cycle but abruptly increase at the checkpoint they most contribute to (G 1 cyclins are an exception, as they are required throughout the cell cycle). (jove.com)
Cyclins function as regulators of CDK kinases. (novusbio.com)
Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. (novusbio.com)

M, Peterson C. 2018 Haematopoietic stem suggests that during differentiation a stem cell approaches a stable equili- cells: entropic landscapes of differentiation. (lu.se)
Single cells in these populations exhibit different com- stem cell differentiation, Shannon information binations of regulator activity that suggest the presence of multiple theory, entropy configurations of a potential differentiation network as a result of multiple entry points into the committed state. (lu.se)
Neuronal differentiation and cell-cycle programs mediate response to BET-bromodomain inhibition in MYC-driven medulloblastoma. (cancerindex.org)
Let-7 Let-7 has an essential role in cell cycle and the differentiation of cell cycle terminals. (ukessays.com)
Osteocytes have recently been identified as a new regulator of bone remodeling, but the detailed mechanism of their differentiation from osteoblasts remains unclear. (lu.se)
The purpose of this study is to identify cell cycle regulators involved in the differentiation of osteoblasts into osteocytes and determine their physiological significance. (lu.se)
The study uses IDG-SW3 cells as a model for the differentiation from osteoblasts to osteocytes. (lu.se)
Among the major cyclin-dependent kinases (Cdks), Cdk1 is most abundantly expressed in IDG-SW3 cells, and its expression is down-regulated during differentiation into osteocytes. (lu.se)
Inhibition of CDK1 activity reduces IDG-SW3 cell proliferation and differentiation into osteocytes. (lu.se)
Pthlh expression increases during differentiation, but inhibiting CDK1 activity reduces Pthlh expression. (lu.se)
Embryogenesis involves orchestrated processes of cell proliferation and differentiation. (biologists.com)
Embryogenesis depends on the timely proliferation of progenitor cells and their subsequent differentiation into multiple cell types. (biologists.com)
In many organs, cell proliferation and differentiation are antagonistically regulated by multiple basic helix-loop-helix (bHLH) activator and repressor genes. (biologists.com)
For example, in the developing nervous system of mouse embryos,progenitor cells proliferate and sequentially give rise to different types of cells by changing their differentiation competency. (biologists.com)
In this primer, we describe the key features of Hes factors and detail their roles in some representative processes of embryogenesis: namely, in the development of the nervous and digestive systems, two well-characterized processes, where Hes1 (and Hes3 and Hes5 in the nervous system) regulates cell proliferation and differentiation, and in the process of somite segmentation, where Hes7 functions as a biological clock. (biologists.com)

In particular, ubiquitin-mediated degradation is critically important at transition points where it provides directionality and irreversibility to the cell cycle, which is essential for maintaining genome integrity. (intechopen.com)
In eukaryotes, many lncRNAs have been identified and shown to be pivotal regulators of genome structure and gene expression. (biorxiv.org)
In gastric carcinoma cells, EBV is not integrated into the host genome but maintained as a type of plasmid called an episome. (spandidos-publications.com)

The programmes governing the function and fate of cells are to a large extent driven by the coordinated activity of transcription factors forming complex and dynamic gene regulatory networks (GRNs). (lu.se)
The activities of transcription factors and other genes involved in cell fate decisions can be measured by a number of different gene expression quantification experiments. (lu.se)
These include genes involved in energy production, intermediary metabolism and cell signaling/transcription factors (0.4 - 0.7 -fold down regulated). (cdc.gov)
The regulatory genes primarily the transcription factors that exert their activity by binding to certain cis DNA elements and consequently either up regulated or down regulate to target expression. (scielo.br)
These transcription factors are known as masters regulators because its single transcript regulate more than one gene, in this context the regulon word is fascinating more in compass of transcription factors. (scielo.br)
To coordinate expression of virulence genes during infection, S. aureus uses 2-component systems, transcription factors ( 5 ) and regulatory or small RNAs (sRNAs), which function as positive ( 6 ) or negative ( 7 ) virulence determinants. (cdc.gov)
Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)

In the present study, polysaccharides composed mainly by β-glucans, produced by the mushroom Gymnopilus imperialis (from Atlantic Forest in Brazil), were evaluated in terms of in vitro immunomodulatory potential, iNOS expression levels, and NO production. (mdpi.com)
Mice lacking the gene for Dmp1 commonly develop lung tumors, and Dmp1 has been identified as a regulator of the Arf-p53 pathway in vitro. (eurekalert.org)
In this study, we examined the antitumor effects of GS-002 in human hepatoma Hep3B and HepG2 cells in vitro . (hindawi.com)
We tested whether natural killer cells expanded by co-culture with K562 cells transfected with 41BBL and membrane-bound interleukin-15 could kill myeloma cells with a high-risk gene expression profile in vitro and in a unique model which recapitulates human myeloma. (haematologica.org)
Indeed, Hbp1 accumulates in cells lacking GID/CTLH activity, and Hbp1 physically interacts and is ubiquitinated in vitro by reconstituted GID/CTLH complexes. (elifesciences.org)
Moreover, inhibition of miR-222-3p can suppress OS cell proliferation, colony formation, and invasion in vitro. (biovendor.com)
In this manuscript, we report the in vitro mutagenicity of waterpipe smoke condensate (WSC), the alteration in cellular parameters of lung alveolar cells in response to WSC exposure and discuss the implication of cellular responses in the pathophysiology of chronic obstructive pulmonary disease (COPD). (who.int)
Nutlin-3 and VPA in combination was found to show a synergistic effect in vitro in an AML cell line expressing wild type p53 (MOLM-13). (uib.no)

1 Significant advances have been made by combining novel agents with autologous peripheral blood stem cell transplantation which allows for long-term disease-free survival in the majority of transplant-eligible patients. (haematologica.org)
High-dose melphalan followed by autologous peripheral blood stem cell transplantation may also be used. (msdmanuals.com)

and syncytial blastoderm mitotic cell cycle. (nih.gov)

Interestingly, the nitric oxide production was significantly increased in treated samples, and the expression of inducible nitric oxide synthase (iNOS) showed that the fraction with β-glucans from G. imperialis induces M1 polarization phenotype. (mdpi.com)
ATF-3 induces DNA synthesis and expression of cyclin D1 in hepatocytes [ 21 ] and is involved in serum-induced cell proliferation as a target gene of c-myc [ 10 ]. (hindawi.com)
Similar to our previous observations in human bladder cancer cells, Boswellia sacra essential oil induces breast cancer cell-specific cytotoxicity. (biomedcentral.com)

By default, CDKs are always present in a cell in an inactivated form. (jove.com)

Cell cycle regulator p27Kip1 was identified as a target of miR-221/miR-222. (biovendor.com)
It was shown that in pancreatic cells, p27Kip1 and miR-221/222 expression levels inversely correlated and that miR221/222 overexpression had important consequences on the proliferation rate and the cell cycle phase distribution. (biovendor.com)

Avoid freeze-thaw cycles. (novusbio.com)

Such heterogeneity could originate from extrinsic factors, such as cell-to-cell signalling and surrounding temperature and pressure, but also from the intrinsic noise generated by having few copies of molecules involved in transcription and translation. (lu.se)

GXD's primary emphasis is on endogenous gene expression during development. (jax.org)
transplanted a population of uSPGs enriched in these cells into the testes of mice treated with busulfan, a toxic compound that kills endogenous germ cells. (elifesciences.org)
These mice are devoid of endogenous natural killer and T-cell activity and were used to determine whether adoptively transferred expanded natural killer cells could inhibit myeloma growth and myeloma-associated bone destruction. (haematologica.org)
Many ncRNAs have critical roles in tumorigenesis, and some ncRNAs function as competing endogenous RNAs (ceRNAs) to perturb gene expression. (biomedcentral.com)
These dCas9-activator fusions targeted to the promoter region of endogenous genes can then modulate gene expression 7 - 11 . (cdc.gov)

In contrast, expression of p27 Kip1 within the primary tumors was not identified to reveal any prognostically important information. (uni-luebeck.de)
Many tumor cell lines and experimental tumors respond to HDAC inhibition. (aacrjournals.org)
Adoptive transfer of expanded natural killer cells inhibited the growth of established OPM2 and high-risk primary myeloma tumors grown in the murine model. (haematologica.org)

A cells cycle is positively regulated promoting progress through the stages via the interaction of two classes of proteins found in the cytoplasm. (jove.com)
It is induced by p53 in response to DNA damage, or by sublytic levels of complement system proteins that result in activation of the cell cycle. (wikipedia.org)
The protein also translocates to the nucleus in response to treatment with complement system proteins, and can associate with and increase the kinase activity of cell division cycle 2 protein. (wikipedia.org)
I am a plant cell biologist and protein biochemist at Oxford Brookes University with expertise in the structure and function of the plant endoplasmic reticulum (ER), membrane proteins and auxin biosynthesis using biochemical techniques as well as high-resolution live cell imaging. (brookes.ac.uk)
Furthermore, silibinin inhibited the migration and invasion abilities of YD10B and Ca9-22 cells by regulating the expression of proteins involved in the epithelial-mesenchymal transition. (jcancer.org)

In addition, Plk1 also prevented partial epithelial-mesenchymal transition (pEMT) of tubular epithelial cells via autophagy pathway. (nature.com)
We have demonstrated that the hDMP1 gene is hemizygously deleted in a significant percentage of mouse and human non-small-cell lung carcinomas, especially those which retained the intact Arf-p53 pathway," explains Dr. Inoue. (eurekalert.org)
Program access grant to the STFC Harwell Laser Facility 'The Plant Cell Initiative: Protein interactions in the higher plant secretory pathway' 2017-2021 (approximate value of £200K). (brookes.ac.uk)
In breast cancer, ATF-3 enhances cancer cell-initiating features [ 22 ] and is associated with activation of the canonical Wnt/ β -catenin pathway [ 23 ]. (hindawi.com)
Western blotting revealed that silibinin downregulated SOD1 and SOD2 and triggered the JNK/c-Jun pathway in oral cancer cells. (jcancer.org)
This leads to activation of p53 and the p53 pathway in cancer cells with wild type p53, subsequently inhibiting tumour growth. (uib.no)

abstract = "Background: The expression of the negative cell cycle regulator p27 Kip1 is frequently found to be deregulated in various human cancer types. (uni-luebeck.de)

Thus, the secretion of gall wasps stimulates plant cell growth and overrules homeostasis of the affected leaf area - this is similar to tumour outgrowth. (nature.com)
The phenomenon of field cancerization (the existence of histologically abnormal tissue beyond a neoplastic area that predisposes to tumour formation) was described in the 1950s on the basis of analyses of squamous cell carcinomas (SCCs). (researchgate.net)
p53 is a tumour suppressor protein involved in maintenance of genomic stability of the cell. (uib.no)

Therefore, in the present investigation the expression of protein within different tissue areas obtained from renal cell carcinomas, their invasion front and corresponding histologically benign renal parenchyma was determined and statistically correlated with several tumor and patient characteristics including the disease-specific long-term survival following surgical treatment. (uni-luebeck.de)
Conclusion: Our analysis demonstrated that p27 Kip1 is heterogeneously expressed in renal cell carcinomas. (uni-luebeck.de)

In the context of DNA damage, p53 is thought to be a decision-making transcription factor that selectively activates genes as part of specific gene expression programmes to determine cellular outcomes. (nih.gov)
Suppression of cellular network formation and disruption of spheroid development of breast cancer cells by Boswellia sacra essential oil suggest that the essential oil may be effective for advanced breast cancer. (biomedcentral.com)
Our biochemical and cellular analysis thus demonstrates that the GID/CTLH complex prevents cell cycle exit in G1, at least in part by degrading Hbp1. (elifesciences.org)
In these processes, Hes genes function as effectors of Notch signaling, which coordinates cellular events via cell-cell interactions. (biologists.com)

New evidence in mice suggests that cells expressing the transcription factor FOXC2 may form a reservoir of quiescent stem cells that contributes to sperm formation. (elifesciences.org)
This involves spermatogonial stem cells (SSCs) dividing to form undifferentiated spermatogonia (uSPGs), which then progress through the meiotic and haploid phases of spermatogenesis to form mature sperm ( de Rooij, 1998 ). (elifesciences.org)
To assess the role of an individual HDAC isoenzyme in physiology and tumor development, HDAC2-mutant mice were generated from a gene trap embryonic stem cell clone. (aacrjournals.org)
The theoretical basis for marrow failure includes primary defects in or damage to the stem cell or the marrow microenvironment. (medscape.com)
External insults (eg, infections, radiation, drugs) may disrupt stem cell homeostasis in marrow environment, leading to altered growth. (medscape.com)
Hematopoietic stem cells (HSCs) enable hematopoietic stem cell transplantation (HCT) through their ability to replenish the entire blood system. (lu.se)

Mutant embryonic fibroblasts fail to respond to insulin-like growth factor I (IGF) by the IGF-I-induced increase in cell number observed in wild-type cells. (aacrjournals.org)

We have generated a mouse model, in which MTC proliferation is induced upon conditional overexpression of the CDK5 activator, p25, in C-cells, and arrested by interrupting p25 overexpression. (oncotarget.com)
Importantly, Dmp1 overexpression inhibited growth of human lung cancer cells with K-ras mutation and wild-type Arf and p53. (eurekalert.org)

However, it is difficult to capture the complexity of transcript isoform variance using these libraries, and large cDNA sequences are often difficult to clone into size-limited viral expression vectors. (cdc.gov)

These genes are regulated by specific regions near the genes called imprinting control regions (ICRs), which contain epigenetic marks (methylation) that coordinate gene expression. (medscape.com)
BWS is caused by genetic or epigenetic changes that disrupt the parent-of-origin specific expression of these genes. (medscape.com)
This process is mediated by various enzymes modifying chromatin structures to achieve the diversity of epigenetic space and the intricacy in gene expression files. (frontiersin.org)
Increasing evidence now suggests a major role for epigenetic mechanisms in gene expression in the parasite. (biorxiv.org)

The tumor suppressor gene p16/cyclin-dependent kinase inhibitor-2A (CDKN2A) is located within the chromosomal region 9p21 and encodes a cell-cycle protein that is an inhibitor of cyclin-dependent kinases (CDK)-4 and -6. (iiarjournals.org)

At these checkpoints, positive and negative regulators promote or inhibit a cell's continuation through the cell cycle. (jove.com)

A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly. (cancerindex.org)

Here, a cDNA array was used to examine differential gene expression in a tibialis anterior (TA) muscle injury mouse model in ( order to gain an insight to the early mediators involved in skeletal muscle injury/repair mechanisms. (cdc.gov)
The microarray analysis, including 715 genes, was conducted ill triplicate and only genes modulated by the injury showing a differential expression (both increased and decreased) 1.7-fold or greater (p (cdc.gov)

Taken together, this study provides novel insights into the dynamic nature of FRT memory CD4 T cells and identifies the menstrual cycle as a key regulator of immune surveillance at the site of STI-pathogen exposure. (cdc.gov)
Here we investigated the contribution of the menstrual cycle to HIV susceptibility through evaluating immune behavior with infection-risk over time. (cdc.gov)
METHODS: Blood and vaginal lavage samples were collected from 18 pig-tailed macaques to evaluate immune changes over reproductive cycles, and from 5 additional animals undergoing repeated vaginal exposures to simian HIV (SHIV). (cdc.gov)
FINDINGS: Macaques cycle-phases were associated with fluctuations in systemic immune properties and a type-1 inflammatory T cell response with corresponding CCR5+ memory CD4 T cell (HIV target cell) infiltration into the vaginal lumen at the late luteal phase. (cdc.gov)

Results: In univariate survival analysis, decreased expression of p27 Kip1 within tissue cores obtained from the invasion front was significantly correlated with the patients' disease-specific long-term survival (p=0.02, log rank test). (uni-luebeck.de)
The signature of our top screening hits is significantly correlated with gene expression data from clinical melanoma samples. (cdc.gov)

Immunocytochemistry/ Immunofluorescence: Cyclin H Antibody (1B8) [H00000902-M01] - Analysis of monoclonal antibody to CCNH on HeLa cell. (novusbio.com)
Western Blot: Cyclin H Antibody (1B8) [H00000902-M01] - CCNH monoclonal antibody (M01), clone 1B8 Analysis of CCNH expression in HeLa. (novusbio.com)
Antibody reactivity against cell lysate and recombinant protein for WB. (novusbio.com)
WB analysis of HeLa cell lysate using GTX33611 Cyclin D1 antibody [RM241]. (genetex.com)
IHC-P analysis of human mantle cell lymphoma tissue using GTX33611 Cyclin D1 antibody [RM241]. (genetex.com)
Antibody- and T-cell-based approaches to targeting of B-cell maturation antigen have shown efficacy. (msdmanuals.com)

This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. (genetex.com)

Many aspects of Hes expression are regulated by Notch signaling, which mediates cell-cell communication. (biologists.com)

J Cell Biol, 2007 Jun 18. (nih.gov)

In 11 cases, there were two cell populations, one with polysomy 9 and the other with monosomy 9. (iiarjournals.org)
Peripheral blood mononuclear cell (PBMC) samples from healthy women (n=10) were prospectively collected over the course of a menstrual cycle to profile T cell populations. (cdc.gov)

I am committed to interdisciplinary research, and an example of the successes gained from this approach is the project with Prof A Nabok (Engineering Sheffield Hallam University) using total internal reflection ellipsometry to quantify protein-membrane interactions on native plant membranes and human cell lines. (brookes.ac.uk)

The mammalian Hes basic helix-loop-helix repressor genes play central roles in these processes by maintaining progenitor cells in an undifferentiated state and by regulating binary cell fate decisions. (biologists.com)
In particular,the Hes bHLH repressor genes play an essential role in the development of many organs by maintaining progenitor cells and by regulating binary cell fate decisions. (biologists.com)
Without Hes genes such as Hes1 , however, progenitor cells prematurely differentiate into certain types of neurons only, and are depleted before they have proliferated sufficiently and generated all neuronal and glial cell types. (biologists.com)

Field cancerization is primarily attributed to changes in the epithelium, but whether changes in mesenchymal cells might also have a primary role in the establishment of afield effect is not clear. (researchgate.net)

Low-power view of hematoxylin-eosin-stained bone marrow showing hypocellularity, with increased adipose tissue and decreased hematopoietic cells in the marrow space. (medscape.com)
The clinical presentation of patients with aplastic anemia includes signs and symptoms related to the decrease in bone marrow production of hematopoietic cells. (medscape.com)
Low power, H and E showing a hypocellular bone marrow with increased adipose tissue and decreased hematopoietic cells in the marrow space. (medscape.com)

EBVaGC is defined by monoclonal proliferation of carcinoma cells with latent EBV infection, as demonstrated by EBV-encoded small RNA (EBER) in situ hybridization. (spandidos-publications.com)
The uniformity of the numbers of terminal repeats (TRs) among EBV positive carcinoma cells reflects the clonal origin of a tumor and suggests that EBV is a causative virus for gastric carcinoma ( 6 ). (spandidos-publications.com)

Whether the expression of p27 Kip1 can be used as a prognostic marker for renal cell cancer patients still remains to be clarified. (uni-luebeck.de)
Multiple myeloma (MM) is a malignant plasma cell disorder with debilitating symptoms related to anemia, immunosuppression, bone destruction, and renal failure. (haematologica.org)

Lesions are caused by bone replacement by expanding plasmacytomas or by cytokines that are secreted by malignant plasma cells that activate osteoclasts and suppress osteoblasts, leading to bone loss. (msdmanuals.com)

This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. (genetex.com)

In this context, in the undifferentiated state, the entropy would be large since fewer constraints exist on the gene expression programmes of the cell. (lu.se)
Accepted: 12 September 2018 tiation progresses, gene expression programmes become more and more constrained and thus the entropy would be expected to decrease. (lu.se)

Patients and Methods: For analysis of p27 Kip1 expression in 420 tumor nephrectomy specimens obtained from 420 consecutively included patients, tissue microarrays were used comprising 1260 tissue samples each obtained from the tumor itself, the invasive front as well as the non-malignant surrounding parenchyma. (uni-luebeck.de)

This averaging effect hampered the analysis of finer regulatory mechanisms at the single-cell level, the fundamental unit for any fate decision process. (lu.se)
The first step was for the team to follow the fate of these cells for six weeks following transplantation. (elifesciences.org)

Multiple myeloma is a cancer of plasma cells that produce monoclonal immunoglobulin and invade and destroy adjacent bone tissue. (msdmanuals.com)

Anatomy18

Organisms11

Diseases5

Chemicals and Drugs101

Analytical, Diagnostic and Therapeutic Techniques and Equipment20

Phenomena and Processes67

Disciplines and Occupations1

Information Science3

Apoptosis22

Progression14

Protein serine1

Pathways8

Negative regulator2

Regulation12

Inhibitors3

Arrest4

Chromatin regulators1

Transcriptional5

Cyclins5

Differentiation15

Genome3

Transcription factors7

Vitro8

Autologous peripheral blood stem cell transplant2

Mitotic cell cycle1

Induces3

CDKs1

P27Kip12

Freeze-thaw cycles1

Molecules1

Endogenous5

Tumors3

Proteins5

Pathway6

Abstract1

Tumour3

Carcinomas2

Cellular4

Stem6

Embryonic1

Overexpression2

TRANSCRIPT1

Epigenetic4

Cyclin-depende1

Inhibit1

Reduced cell proliferation1

Differential2

Immune4

Significantly2

Antibody6

Tumor suppressor protein1

Mediates1

Biol1

Populations2

Interactions1

Progenitor3

Mesenchymal cells1

Hematopoietic cells3

Carcinoma Cells2

Renal2

Suppress1

CDK61

Gene expression programmes2

Malignant1

Fate2

Multiple myeloma1