Cell cycle regulation embryonic clone cells. Medical search. Frequent questions

He has been leader of a research group at the Max-Delbrück-Center for Molecular Medicine in Berlin until 2011 focusing his research on signal transduction mechanisms in human and murine embryonic stem cells. (eurostemcell.org)

Conditional Mutation of SMC5 in Mouse Embryonic Fibroblasts. (nih.gov)
We also measured the degree of NF-κB inhibition by piperlongumine and its derivatives in a NF-κB zebrafish reporter line and in mouse embryonic fibroblasts. (creighton.edu)

Promoters of genes associated with oxidative phosphorylation are preferentially remethylated at the 8-cell stage, suggesting that this mode of energy metabolism may not be favored. (nature.com)
DEAF-1 activates the expression of Mtk and Drs promoter-luciferase fusion genes in S2 cells. (sdbonline.org)
The expression of cyclin-dependent kinase inhibitors was profoundly affected with early activation and then repression of p21 cip1/waf1 and persistent activation of both p27 kip1 and p57 kip2 , whereas genes involved in cell survival and proliferation were suppressed. (aacrjournals.org)
Enriched expression of genes in LRECb was associated with stem cell attributes and identified WNT, TGF-β, and MAPK pathways of self renewal and proliferation. (frontiersin.org)
Genes expressed in LRECe revealed retention of some stem-like properties along with up-regulation of differentiation factors. (frontiersin.org)
whereas LREC in suprabasal epithelial layers are enriched for more committed progenitor cells, expressing some genes that are associated with stem cell attributes along with those indicative of cell differentiation. (frontiersin.org)
Using a genome-wide chromatin immunoprecipitation cloning technique, we identified and validated 14 novel p53-regulated genes following NGF treatment. (biomedcentral.com)
Furthermore, we describe stimulus-specific regulation of a subset of these target genes by p53. (biomedcentral.com)
The cell cycle is a complex process with myriad genes involved and elaborate and complex signaling mechanisms to allow for this critical cellular process. (biomedcentral.com)
The indicated mutants (all are conditional, temperature sensitive) were transformed with either the empty vector pAid or pAid clones bearing nudE and nudF6 genes (plasmids recovered in the multicopy suppressor screen) and grown at 32°C. Transformants were gridded on YAG plates with or without KCl and incubated at 43°C or 32°C for 2 d. (xenbase.org)
The underphosphorylated, active form of Rb interacts directly with E2F1 , leading to cell cycle arrest, while the hyperphosphorylated form decouples from E2F1, thus promoting the transcription of genes promoting entry into the S phase. (biolegend.com)
The methodology included inducing differentiated somatic cells with the primary genes responsible for embryonic stem cell potency. (papersowl.com)
Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)
Below you can see some examples of the infrastructure for research on genes and cells, available for researchers at Lund University. (lu.se)
In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)

Is the Subject Area "Cell differentiation" applicable to this article? (plos.org)
Furthermore, we used this assay to determine the effects of BRCA1 variants on cell cycle regulation, differentiation, and genomic stability. (jci.org)
This dictates cycles of mammary growth, differentiation, lactation, and regression, during which mammary stem cells (MaSC) provide for the lineages of luminal and basal (myoepithelial) epithelial cells in the ducts and alveoli. (frontiersin.org)
Mounting evidence also suggests a role for p53 in differentiation of cells including neuronal precursors. (biomedcentral.com)
We studied the transcriptional role of p53 during nerve growth factor-induced differentiation of the PC12 line into neuron-like cells. (biomedcentral.com)
We hypothesized that p53 contributed to PC12 differentiation through the regulation of gene targets distinct from its known transcriptional targets for apoptosis or DNA repair. (biomedcentral.com)
The data show p53 protein was transcriptionally activated and contributed to NGF-mediated neurite outgrowth during differentiation of PC12 cells. (biomedcentral.com)
p53 transcriptional activity may be further involved in the differentiation of other non-neuronal cell types. (biomedcentral.com)
Furthermore, the decreased expression of the EZH2 gene is crucial for stem cell differentiation into specific cell lineages involved in myogenesis, adipogenesis, osteogenesis, neurogenesis, and haematopoiesis [ 9 ]. (hindawi.com)
Our scientists have developed a wide array of stem cell-focused reagents and resources for many applications including flow cytometry , western blotting , ELISAs , and recombinant proteins for cell differentiation. (biolegend.com)
RUNX1 is also required for the differentiation of CD8+, Th17, and regulatory T cells. (biolegend.com)
These findings revealed that RUNX1 acts as a tumor suppressor for myeloid leukemia and is crucial for the development and terminal differentiation of several blood cell lineages 2,3 . (biolegend.com)
A stem cell commences as an undifferentiated cell that can either undergo self-renewal, whereby it generates daughter cells that remain as stem cells, or mature into a specific cell type via differentiation (Can/Hematol 2008). (papersowl.com)

These results validated a different transcript in pigs and characterized its expression profile in fetal tissues of different gestation stages, which indicated that EZH2 played important roles during porcine embryonic development. (hindawi.com)
In this study, we identified a transcript variant of EZH2 in porcine fetal tissues by cloning and sequencing. (hindawi.com)
Mechanochemical Principles of Spatial and Temporal Patterns in Cells and Tissues. (mpi-cbg.de)
Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
Totipotent stem cells contain all the constituents necessary to produce a living being, given that these cells can supply all embryonic and extraembryonic tissues required for proper growth (Can/Hematol 2008). (papersowl.com)
It is a complex process that affects various tissues, one of them being the bone marrow, wherethrough the interactions of the hematopoietic stem cell (CTH) with its surrounding environment, including with the mesenchymal stem cell (CTM), hematopoiesis takes place. (bvsalud.org)
Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)

In somatic cells, the activity of telomerase, a reverse transcriptase that can elongate telomeric repeats, is usually diminished after birth so that the telomere length is gradually shortened with cell divisions, and triggers cellular senescence. (nature.com)
Thus, even in stem cells, except for embryonal stem cells and cancer stem cells, telomere shortening occurs during replicative ageing, possibly at a slower rate than that in normal somatic cells. (nature.com)
Telomeric DNA consists of short guanine-rich repeat sequences in all eukaryotes with linear chromosomes, and its length in human somatic cells is remarkably heterogeneous among individuals ranging from 5 to 20 kb, according to age, organ, and the proliferative history of each cell ( Wright and Shay, 2005 ). (nature.com)
In most human somatic cells except for stem cells and lymphocytes, telomerase activity is diminished after birth so that telomere length shortens with each cell division. (nature.com)
While CTCF is essential for embryonic development, little is known of its absolute requirement in somatic cells and the consequences of CTCF haploinsufficiency. (preprints.org)
In contrast, adult stem cells can only give rise to differentiated somatic cells of the particular tissue from which these cells originated (Can/Hematol 2008). (papersowl.com)

Western blot analysis of HEK293 embryonic kidney cell line whole cell lysate probed with Mouse anti Human c-myc antibody ( MCA2200 ) followed by HRP conjugated Goat anti Mouse IgG secondary antibody visualized using chemiluminescence. (bio-rad-antibodies.com)

Zygotic mutants develop to pupal stages, but do not eclose, whereas maternal mutants display severe defects in early embryonic patterning (Veraksa, 2002). (sdbonline.org)
and from 2002 on pluripotency in embryonic stem cells in the lab of Ali H. Brivanlou. (eurostemcell.org)

The process of isolating these cells has been controversial, because it typically results in the destruction of the embryo. (wikipedia.org)
Somatic cell nuclear transfer is a cloning method that can be used to create a cloned embryo for the use of its embryonic stem cells in stem cell therapy. (wikipedia.org)
In 1-cell and 2-cell embryos Dnmt1s is derived from the oocyte, whereas from the 2-cell stage onward the embryo starts to synthesize its own Dnmt1s 8 . (nature.com)
In the early embryonic development, abnormal expression of EZH2 impaired embryo growth and pluripotency maintenance [ 7 , 8 ]. (hindawi.com)
A pluripotent stem cell has the potential to form all cells of the embryo, but cannot form a placenta. (papersowl.com)

In order to distinguish the rate of proliferation between different PHM clones, a comparative index (CI) was established using the cell cycle and total RNA data of the two PHM clones. (sun.ac.za)
Finally, FGF6 and FGF2, both individually and sequentially, were used to treat quiescent myoblasts to determine their involvement in activation and proliferation with the use of cell cycle analysis and mRNA assessment of ki67, p21, myf5, and MyoD. (sun.ac.za)
Results and conclusion: The development of the CI was successful in determining the difference in proliferation rate for the different clones. (sun.ac.za)
However, FGF2 did impede the cell cycle inhibition factor p21, indirectly influencing proliferation. (sun.ac.za)
The inverse sequential treatment order did not demonstrate any significant effect on both activation and proliferation of the quiescent cells. (sun.ac.za)
During rapid mammary growth in the mouse, label retaining epithelial cells (LREC) appear to retain label by asymmetric distribution of DNA strands, as evidenced by a rapid proliferation index of the LREC ( Smith, 2005 ). (frontiersin.org)
During periods of low mammary proliferation, quiescence of the stem cell population may account for retention of label. (frontiersin.org)
Long noncoding RNAs (lncRNAs) regulate a number of physiological and pathological processes, including gene transcription and translation, chromatin modification, cell cycle progression, cell proliferation, and oncogenic and tumor-suppressive signals in cancer 6 . (researchsquare.com)
Retroviral vectors expressing short hairpin RNAs (shRNAs) against CDC2 caused efficient deletion of CDC2, cellular G2/M arrest concluding in apoptosis and inhibition of proliferation in human glioma cells U251 and SHG-44 cell lines ex vivo. (biomedcentral.com)
Bowen, 2004} detected keratinocyte proliferation by PCNA (proliferating cell nuclear antigen) marking, 13,9% of keratinocytes were stained, compared with 6,4% in normal skin. (globale-dermatologie.com)

CCCTC-binding factor (CTCF) is a conserved transcription factor that performs diverse roles in transcriptional regulation and chromatin architecture. (preprints.org)
Positional cloning efforts revealed that this locus contains the SWI/SNF related, matrix-associated, actin-dependent regulator of chromatin, subfamily B, member 1 ( SMARCB1 ) gene, also known as human sucrose non-fermenting gene number 5 ( hSNF5 ), integrase interactor 1 ( INI1 ) , or 47-Kd Brg1/Bam -associated factor ( BAF47 ). (medscape.com)

Unlike FGF2, individual treatment with FGF6 was adequate to activate the quiescent PHMs and aid their re-entry into cell cycle with consistency in all three PHM clones by upregulating ki67 expression. (sun.ac.za)
The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. (thermofisher.com)
We show here that expression of EBF3 resulted in cell cycle arrest and apoptosis. (aacrjournals.org)
For example, increased expression of LINC00958 attributes to cell metastasis in gastric cancer 7 . (researchsquare.com)
Retroviral vectors expressing short hairpin RNAs (shRNAs) targeted against CDC2 were designed and transducted into human glioma cell line ex vivo in order to downregulate the expression of CDC2. (biomedcentral.com)
Western Blotting was used to determine the level of expression of CDC2 protein as measure to quantify down regulation of CDC2 expression along with use of flow cytometry to investigate effect of shRNAs on cell cycles and detection of apoptosis. (biomedcentral.com)
Thus, the appropriate expression level of the EZH2 gene is important for embryonic development. (hindawi.com)
However, the transcript and expression status of the EZH2 gene during porcine embryonic development remains unknown. (hindawi.com)
Subsequently, we detected the expression of EZH2 on mRNA level and protein level in two different embryonic development stages (65-dpc and 90-dpc) via qRT-PCR and western blots. (hindawi.com)
This thesis aimed to 1) lower the cost of production by using cheap C1 carbon sources (e.g. methane) from agricultural activities (e.g. intensive dairy, piggeries, etc.) for the cultivation of transformed yeast and 2) to assess the therapeutic efficacy of recombinant hIFNγ in its glycosylated and non-glycosylated form from different expression systems against ovarian cancer cells. (edu.au)
As shown by ELISA, amino acid starvation-induced selective pressure on HIS4 improved expression and secretion of the adjacent hIFNγ by 55% compared to unchallenged cells. (edu.au)
The capability of these cells to differentiate depends on the stem cell type, the regulation of gene expression by various transcription factors and interaction with the stem cell niche 1,4 . (biolegend.com)
Alexa-FluorA647 conjugated Mouse anti Human c-myc antibody, clone 9E10 ( MCA2200A647 ) used for the evaluation of c-myc on dicidual NK cells demonstrating the correlation between c-myc and CD122 expression. (bio-rad-antibodies.com)
Let us underline nevertheless that such cytokeratin expression is found in embryonic cell lines and that the study only concerned 10 specimens. (globale-dermatologie.com)

Deformed epidermal autoregulatory factor-1 (DEAF-1) is a transcription factor that was originally shown to bind the autoregulatory enhancer of the Deformed ( Dfd ) Hox gene, which is activated in embryonic head segments of Drosophila (Gross, 1996). (sdbonline.org)
Chromosome association of minichromosome maintenance proteins in Drosophila mitotic cycles. (colorado.edu)
Cloning of Drosophila MCM homologs and analysis of their requirement during embryogenesis. (colorado.edu)

In mammals, roughly 50-150 cells make up the inner cell mass during the blastocyst stage of embryonic development, around days 5-14. (wikipedia.org)
However, robust and large-scale genome-wide reprogramming of DNA methylome occurs during two critical developmental processes: (1) development of primordial germ cells and (2) pre-implantation embryogenesis. (nature.com)
Monkeys have served as one of the most valuable models for understanding DNA methylation dynamics during early embryogenesis in human due to their similarities in genetics and early embryonic development 17 , 18 . (nature.com)
Furthermore, due to ethical and legal concerns, very limited techniques can be applied to human embryos to validate some of significant conclusions drawn from descriptive studies regarding human embryonic development. (nature.com)
Fibroblast Growth Factors (FGFs) are fundamental in embryonic development but also in adult skeletal muscle regeneration from injury or pathology. (sun.ac.za)
SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. (thermofisher.com)
The thymus plays a central role in the development of the body's immune system as it is the main site in which T cells are generated. (eurostemcell.org)
We show that the enzymatic acetylation and deacetylation of a cell surface carbohydrate controls B cell development, signaling, and immunological tolerance. (harvard.edu)
In this study, we evaluated the effect of heat stress (HS) on the development of cells secreting major reproductive hormones of the HPG axis (i.e., hypothalamus, pituitary gland, ovary, and testis) of Wenchang chicks. (scielo.br)
In brief, HS caused dysfunction of the corresponding target organs of the HPG axis in Wenchang chicks, and this affected the normal growth and development of the cells' HPG axis. (scielo.br)
Impact of maternal heat stress in conjunction with dietary zinc supplementation on hatchability, embryonic development, and growth performance in offspring broilers. (scielo.br)
EZH2, a methyltransferase catalyzing H3K27me3, has been abundantly studied in human and mouse embryonic development. (hindawi.com)
In pigs, several studies have focused on its function in early embryonic development regulation and in SCNT efficiency improvement [ 8 , 14 - 16 ]. (hindawi.com)
Our study is aimed at providing information for understanding the biological function of EZH2 in porcine embryonic development. (hindawi.com)
These results suggest that maintaining MPF activity after cryopreservation may have a positive effect on further embryonic development, but is unable to fully overcome cryoinjury. (molcells.org)
We examined the consequences of CTCF depletion in immortalised human and mouse cells using shRNA knockdown and CRISPR/Cas9 genome editing and examined the growth and development of heterozygous Ctcf ( Ctcf +/- ) mice. (preprints.org)
RUNX1 regulates CD4 gene transcription during multiple stages of T cell development and represses the CD4 gene in CD4-CD8- (double negative) T cells. (biolegend.com)
Pax6 is a transcription factor present during embryonic development. (biolegend.com)
Within the brain, the protein is involved in the development of specialized cells that process smell. (biolegend.com)
Increases in the amount of PAPP-A mRNA in granulosa cells during follicular development occurs in some but not all species, indicating that other proteases or protease inhibitors may be involved in IGFBP degradation. (bioone.org)
Overall, the presence of stem cells is essential for proper human development and function as they contribute to the growth, maintenance, and repair of numerous physiological systems (Kara et al. (papersowl.com)

Conditional mutation of Smc5 in mouse embryonic stem cells perturbs condensin localization and mitotic progression. (nih.gov)
The tumor suppressor p53 is recognized as a critical regulator of cell cycle progression and apoptosis, incorporating signals from DNA damage and other cellular stressors to decide cell fate [ 1 ]. (biomedcentral.com)
Abnormalities in cell cycle regulation are reported to be strongly associated with tumorigenesis and progression of tumors. (biomedcentral.com)

Transcription factors have an important role in the ability of a cell to self-renew and also differentiate into most cell types, also known as pluripotency 1 . (biolegend.com)
This pluripotency enables these cells to produce daughter cells of all differentiated somatic cell types, germ cells, and cells of all three embryonic germ layers (Can/Hematol 2008). (papersowl.com)

Analysis of the effectiveness of caffeine and MG132 for improving somatic cell nuclear transfer (SCNT) technology using cryopreserved eggs showed that supplementation did not improve the blastocyst formation rate of cloned mouse eggs. (molcells.org)

Since 1998 however, it has been possible to culture and differentiate human embryonic stem cells (in stem-cell lines). (wikipedia.org)
By 1998, human embryonic stem cells were first isolated by American biologist James Thomson, which made it possible to have new transplantation methods or various cell types for testing new treatments. (wikipedia.org)
Recently, the importance of telomere maintenance in human stem cells has been highlighted by studies on dyskeratosis congenital, which is a genetic disorder in the human telomerase component. (nature.com)
The regulation of telomere length and telomerase activity is a complex and dynamic process that is tightly linked to cell cycle regulation in human stem cells. (nature.com)
Here we review the role of telomeres and telomerase in the function and capacity of the human stem cells. (nature.com)
In fact, low levels of telomerase activity have been found in human adult stem cells including haematopoietic and non-haematopoietic stem cells such as neuronal, skin, intestinal crypt, mammary epithelial, pancreas, adrenal cortex, kidney, and mesenchymal stem cells (MSCs) ( Table 1 ). (nature.com)
Basically, given the difference of telomere and telomerase activity in human and mouse cells, the telomere and telomerase status in stem cell populations is different between humans and mice ( Harrington, 2004 ). (nature.com)
Methods: Isolated satellite cells from human muscle biopsies were expanded in vitro creating primary human myoblast (PHM) clones. (sun.ac.za)
We introduced BAC clones with human wild-type BRCA1 or variants into Brca1-null ES cells and confirmed that only wild-type and a known neutral variant rescued cell lethality. (jci.org)
In this issue of the JCI , Chang, Sharan, and colleagues describe a novel system to evaluate human BRCA1 alleles for in vivo function using BACs containing human BRCA1 vectors in mouse cells and embryos (see the related article beginning on page 3160). (jci.org)
The KEGG and GO analyses indicated 'Human T − cell leukemia virus 1 infection' and 'Hippo signaling pathway' were the main affected biological processes. (researchsquare.com)
Downregulation of CDC2 could potentialy inhibit human gliomas cells growth ex vivo and in vivo. (biomedcentral.com)
To reveal the roles of CDC2/Cyclin B1 in human malignant gliomas, CDC2 in glioma cell line, it was down-regulated by retrovirus vectors expressing short hairpin RNAs (shRNAs) ex vivo and in vivo. (biomedcentral.com)
Rb homeostasis is also essential for self-renewal and survival of human embryonic stem cells 10 . (biolegend.com)
The Host Cell Reactivation assay (HCR) allows studying the DNA repair capability in different types of human cells. (biomedcentral.com)
Previously we have shown that DDB2, a protein involved in the Global Genome Repair, interacts directly with PCNA and, in human cells, the loss of this interaction affects DNA repair machinery. (biomedcentral.com)
To this end, human kidney 293 stable clones, expressing DDB2 Wt or DDB2 PCNA- , were co-transfected with pmRFP-N2 and UV-irradiated pEGFP-reported plasmids. (biomedcentral.com)
This method allows studying the DNA repair capability in different types of human cells [ 11 ] and may be employed as a marker for genetic instability and cancer risk [ 12 , 13 ]. (biomedcentral.com)
Father Frank Pavone, a key proponent of the Roman Catholic Church's pro-life movement, has devoted his life's work to ending abortion, euthanasia, embryonic stem cell research, and other techniques and procedures that he believes threaten human life from conception to death. (asu.edu)
It wasn't until over a decade later, in the late 1990s, that human-derived stem cells were propelled to the forefront of experimentation. (papersowl.com)
Marking a vast leap in reproductive therapy, it became possible to isolate these human embryonic stem cells for reproductive purposes, such as in vitro fertilization. (papersowl.com)

Discovery of non-genotoxic mechanisms through which p53 protein may be activated within tumor cells harboring wild-type p53 remains an important therapeutic objective, both in effort to alleviate traditional off-target chemotherapeutic side-effects and to prevent the unpredictable mutagenicity characteristic of these therapies. (biomedcentral.com)
Chimento A, Sirianni R, Casaburi I, Pezzi V. Role of Estrogen Receptors and G Protein-Coupled Estrogen Receptor in Regulation of Hypothalamus-Pituitary-Testis Axis and Spermatogenesis. (scielo.br)
Rb , or retinoblastoma protein, is a key regulator of the cell cycle, particularly during the transition from the G1 to S phases. (biolegend.com)
To clarify this issue, we decided to apply a transfection-based assay, named Host Cell Reactivation (HCR), to investigate DNA lesions removal efficacy in the presence of DDB2 Wt protein or DDB2 mutated one. (biomedcentral.com)
Although intracellular signal transduction is often portrayed as a protein kinase 'domino effect', the counterbalancing function of phosphatases, and thus the control of phosphatase activity, is equally relevant to proper regulation of cellular function. (ac.be)
In the past, most of the attention was focused primarily on protein kinases and on their regulation, mainly because phosphatases were then viewed as simple housekeeping enzymes. (ac.be)
But advances in the understanding of protein phosphatases make now clear that these enzymes are precisely regulated and are as important as kinases in the regulation of cellular processes involving protein phosphorylation. (ac.be)

p53 is recognized as a critical regulator of the cell cycle and apoptosis. (biomedcentral.com)
Indeed apoptosis is inhibited in senescent cells. (globale-dermatologie.com)

Background: The transcription factor B-Myb is present in all proliferating cells, and in mice engineered to remove this gene, embryos die in utero just after implantation due to inner cell mass defects. (harvard.edu)

Malignant rhabdoid tumor (MRT) was initially described in 1978 as a rhabdomyosarcomatoid variant of a Wilms tumor because of its occurrence in the kidney and because of the resemblance of its cells to rhabdomyoblasts. (medscape.com)

antibody (clone AD2.35) on mouse primary astrocytes. (biolegend.com)
The cells were fixed, permeabilized, blocked and then stained with primary antibody, and Alexa Fluor® 594 conjugated secondary IgG (Cat. (biolegend.com)

In vivo, they eventually differentiate into all of the body's cell types (making them pluripotent). (wikipedia.org)
These approaches result in the loss of all histological information pertaining to the in vivo locale of MaSC and progenitor cells. (frontiersin.org)
Until recently, it was believed that they were tissue-specific…however, this concept has been challenged… (multipotent cells) can differentiate in vitro and in vivo into various cell types not only from the tissue of origin" (Can/Hematol 2008). (papersowl.com)

AVT is important for osmoregulation ( 15 ), and increasing number of neuroanatomical studies reported that AVT is involved in the regulation of sex-related reproductive behavior ( 16 - 18 ). (frontiersin.org)

In that same year, Siminovitch was a lead investigator for studies that found colony-forming cells were capable of self-renewal, which is a key defining property of stem cells that Till and McCulloch had theorized. (wikipedia.org)

Depletion of SMC5/6 sensitizes male germ cells to DNA damage. (nih.gov)

Telomerase can add telomeric repeats onto the chromosome ends, and prevents the replication-dependent loss of telomere and cellular senescence in highly proliferative cells of the germline and in the majority of cancers ( Blasco, 2005 ). (nature.com)
No chromosome was found contrary to an extra chromosome 5 in the basal cell carcinoma. (globale-dermatologie.com)

4 While proteins can be phosphorylated on nine amino acids, serine, threonine and tyrosine phosphorylation are by far the most predominant in eukaryotic cells. (ac.be)

They are found in both embryonic and adult organisms, but they have slightly different properties in each. (wikipedia.org)
Adult stem cells are found in a few select locations in the body, known as niches, such as those in the bone marrow or gonads. (wikipedia.org)
ENGLISH ABSTRACT: Introduction: Adult mammalian tissue regeneration recruits progenitor stem cells. (sun.ac.za)
Stem cells are unique due to their ability to limitlessly self-renew and differentiate into each cell type in the adult body. (biolegend.com)
That is to say, one type of stem cell may be capable of differentiating into all adult cell types, while another may only be capable of maturing into one specific somatic cell type. (papersowl.com)
On a macro scale, the two classifications of stem cells are embryonic and adult stem cells. (papersowl.com)
To delve further into the specifics, embryonic and adult stem cells fall into potency categories reflecting their ability to differentiate: totipotent, pluripotent, multipotent, and unipotent. (papersowl.com)
In the early 1980s, scientists began to exploit two major classes of stem cells: embryonic and non-embryonic, or adult stem cells. (papersowl.com)
However, the crowning achievement arose in 2006 when researchers at Kyoto University discovered the necessary means to reprogram differentiated adult cells to possess embryonic stem cell-like functionality, as if reversing time itself. (papersowl.com)

Other recent studies verified the presence of PAPP-A mRNA in granulosa cells of humans, monkeys, cattle, mice, and pigs. (bioone.org)

Clare's lab recently identified a population of progenitor/stem cells in mice that, on transplantation, is sufficient to generate a fully functional thymus. (eurostemcell.org)

Secondly, the quiescent state is an integral part of stem cell regulation, therefore choosing the right protocol for inducing quiescence is important. (sun.ac.za)

A critical length of telomere repeats is required to ensure proper telomere function and avoid the activation of DNA damage pathways that result in replicative senescence or cell death. (nature.com)
To further identify the potential signaling pathways after the knockdown of LINC00958 in Ishikawa cells, we performed the KEGG and GO analyses (Figure 2). (researchsquare.com)

RUNX1-deficient mice fail to generate hematopoietic stem cells. (biolegend.com)
Hematopoietic stem cells, which can produce several different types of blood cells, are prime examples of multipotency. (papersowl.com)

Insights into the biology of stem cells will be gained by confirmation and characterization of candidate MaSC markers identified in this study. (frontiersin.org)
Before delving into the molecular biology and therapeutic potentials of induced pluripotent stem cells, it is crucial to provide foundational definitions and descriptions. (papersowl.com)

2000. A metabolite of methoxychlor, 2,2-bis( p hydroxyphenyl)-1,1,1-trichloroethane, reduces testosterone biosynthesis in rat leydig cells through suppression of steady-state messenger ribonucleic acid levels of the cholesterol side-chain cleavage enzyme. (cdc.gov)

The risk is increased 1000-fold for squamous cell carcinoma, basal cell carcinoma, malignant melanoma, and fibrosarcoma and is increased 10-fold to 20-fold for other tumors. (medscape.com)

In multicellular organisms, stem cells are undifferentiated or partially differentiated cells that can differentiate into various types of cells and proliferate indefinitely to produce more of the same stem cell. (wikipedia.org)
Our facilities provide the opportunity to study molecules, cells, organs and entire organisms. (lu.se)

3 This article will review the recent advances in the structure and regulation of this fascinating enzyme. (ac.be)

The GnRH secreted from the hypothalamus activates specific receptors on the pituitary gland to stimulate the synthesis and secretion of FSH and LH from gonadotropic cells of the pituitary gland. (scielo.br)

The key properties of a stem cell were first defined by Ernest McCulloch and James Till at the University of Toronto and the Ontario Cancer Institute in the early 1960s. (wikipedia.org)

Silencing of the EBF3 locus was observed in brain, colorectal, breast, liver, and bone tumor cell lines and its reactivation was achieved on treatment with 5-aza-2′-deoxycytidine and trichostatin A in a significant portion of these tumor cells. (aacrjournals.org)

They exist to replenish rapidly lost cell types and are multipotent or unipotent, meaning they only differentiate into a few cell types or one type of cell. (wikipedia.org)
Oligopotent stem cells, though not often widely discussed, have the ability to differentiate into a few cells (Kara et al. (papersowl.com)

Research into stem cells grew out of findings by Canadian biologists Ernest McCulloch, James Till and Andrew J. Becker at the University of Toronto and the Ontario Cancer Institute in the 1960s. (wikipedia.org)
Thus, telomerase activity and telomere maintenance are associated with the immortality of cancer cells, germ-line cells, and embryonic stem (ES) cells. (nature.com)
In our study, we analyzed the effects of the knockdown of LINC00958 in endometrial cancer cells by using the RNA-seq data. (researchsquare.com)

Appropriate regulation of CDC2 is essential for the entry into mitosis. (biomedcentral.com)
The proper methylation status of histones is essential for appropriate cell lineage and organogenesis. (hindawi.com)
hIFNγ was cloned into the pPIC9 vector adjacent to the HIS4 gene, a gene essential for histidine biosynthesis, which was then transformed into P. pastoris. (edu.au)

They discovered the blood-forming stem cell, the hematopoietic stem cell (HSC), through their pioneering work in mice. (wikipedia.org)
McCulloch and Till began a series of experiments in which bone marrow cells were injected into irradiated mice. (wikipedia.org)
They observed lumps in the spleens of the mice that were linearly proportional to the number of bone marrow cells injected. (wikipedia.org)

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