• HT-29 cells, which express dominant negative p53 and show no increase of GTPase activity when treated with morphine, were less sensitive in vitro and were not affected in vivo . (aacrjournals.org)
  • In fact, FAK immunoprecipitated from metabolically-labelled mitotic HeLa cells without tyrosine phosphatase inhibitors was phosphorylated on Ser only and was dephosphorylated in vitro by purified muscle PP1, with loss of phospho-Ser. (biochemj.org)
  • FAK obtained from cells at 1.5h was Ser-phosphorylated, and underwent dephosphorylation during in vitro incubation, with loss of phospho-Ser, indicating the presence of active FAK-bound phosphatase. (biochemj.org)
  • These events were associated with increases in the protein levels of cyclin B1, cyclin D1, cyclin E, cyclin-dependent kinase 1, cyclin-dependent kinase 2, proliferating cell nuclear antigen, and p21 Cip1 in vivo and in vitro, whereas inhibition of the PI 3-kinase signaling pathway with either rapamycin or wortmannin blocked the upregulation of these cell cycle proteins, but not mRNA, and arrested the cells in vitro before S phase. (ahajournals.org)
  • In this study, the Dbf4 protein was shown to be required for Cdc7 kinase activity in that Cdc7 kinase activity is thermolabile in vitro when extracts prepared from a temperature-sensitive dbf4 mutant grown under permissive conditions are used. (asm.org)
  • In vitro reconstitution assays, in addition to employment of the two-hybrid system for protein-protein interactions, have demonstrated that the Cdc7 and Dbf4 proteins interact both in vitro and in vivo. (asm.org)
  • All three CSBP II proteins show specificity for binding the wild-type cycling sequence in vitro. (asm.org)
  • Inhibition of cell growth is the most commonly used endpoint for in vitro toxicity of biomaterials. (lu.se)
  • 69:882-888, 1995), who showed that Vpr prevents the establishment in vitro of chronically infected HIV producer cell lines, apparently by causing infected cells to arrest in the G2/M phase of the cell cycle. (asm.org)
  • Indeed, a fusion protein of N-terminal GRD exhibited GAP activity for Rap1 in vitro, and rather surprisingly also for Ran which is so far the only small GTPase known to be present in the nuclei. (nii.ac.jp)
  • Secondly, we show that suppression of Ect2 reduces viability of chemo- and radio-resistant human glioma cells in vitro. (nus.edu.sg)
  • With IC 50 values in the nanomolar range, these compounds block the ability of Pim to phosphorylate peptides and proteins in vitro and, when added to DU145 prostate cancer cells overexpressing Pim, inhibit the ability of this enzyme to phosphorylate a known substrate, the BH 3 protein BAD. (elsevier.com)
  • Smad nuclear interacting protein 1 (SNIP1) is an evolutionarily conserved protein containing a forkhead-associated (FHA) domain that regulates gene expression through interactions with multiple transcriptional regulators. (nature.com)
  • The S100 family of calcium-binding proteins regulates many aspects of cell function but their roles in vascular physiology are less well understood. (sigmaaldrich.com)
  • Here, we report that the E2Fa transcription factor of Arabidopsis thaliana is an essential component that regulates the asymmetric cell division marking lateral root initiation. (ugent.be)
  • NUB1 is a negative regulator of the NEDD8 conjugation system, which regulates many biological events, including cell cycle transition and apoptosis. (arvojournals.org)
  • SNIP1 itself is a 396 amino-acid protein that contains an N-terminal bipartite nuclear localization signal (NLS) and a putative forkhead-associated (FHA) domain in its C-terminus. (nature.com)
  • PAR pattern of expression and its dynamic localization suggested a functional relationship to chromosomal passenger proteins (CPP). (biomedsearch.com)
  • L1 protein expression and localization ( A ) Representative pictures of immunostained HeLa M2 cells expressing a recoded L1 (ORFeus) or a non-recoded L1 (L1rp). (elifesciences.org)
  • Based on this, the aim of the study was to investigate whether altered localization and/or p53 protein expression is an early event in tumor development in liver that possibly contributes to the growth advantage of initiated hepatocytes. (diva-portal.org)
  • Additionally, we selected one of these newly identified membrane proteins to further characterize in both T. gondii and P. falciparum. (usf.edu)
  • Transport vesicles contain membrane proteins (v-SNAREs), which specifically bind to cognate membrane proteins (t-SNAREs) in the appropriate target membrane. (rupress.org)
  • SM proteins are peripheral membrane proteins. (rupress.org)
  • To explore the molecular basis for the cell cycle-dependent oscillation of Skp2 mRNA, we have now characterized the promoter region of Skp2 . (aacrjournals.org)
  • These findings could inform more effective cancer treatments and help answer larger questions about molecular mechanisms, according to Dileep Varma, PhD , assistant professor of Cell and Molecular Biology and senior author of the study. (northwestern.edu)
  • Dileep Varma, PhD, assistant professor of Cell and Molecular Biology, was the senior author of a study published in the Journal of Cell Biology. (northwestern.edu)
  • 000114857 001__ 114857 000114857 005__ 20181203021031.0 000114857 037__ $$aARTICLE 000114857 245__ $$aA superfamily of conserved domains in DNA damage-responsive cell cycle checkpoint proteins 000114857 269__ $$a1997 000114857 260__ $$c1997 000114857 336__ $$aJournal Articles 000114857 500__ $$aEuropean Molecular Biology Laboratory, Heidelberg, Germany. (epfl.ch)
  • 4 We hypothesized that in addition to the generalized increases in protein synthesis necessary for cell growth, there is a highly regulated increase in the translation of certain mRNA species, including those that encode cell cycle proteins, and we also hypothesized that the molecular machinery regulating this translation represents a potential target for therapeutic intervention. (ahajournals.org)
  • A second distinct binding protein termed CSBP II has been purified from CSBPA null mutant cells, lacking both CSBPA and CSBPB proteins, and contains three major polypeptides with predicted molecular masses of 63, 44.5, and 33 kDa. (asm.org)
  • Accumulated evidence suggests that Ras functions as a molecular switch for reentry of quiescent cells into the cell cycle, which is at least partly dependent on the Raf-MEK1/2-ERK pathway ( 16 , 22 , 33 , 52 ). (asm.org)
  • Rescue of this molecular phenotype is possible by re-expressing human FXR1P in Fxr1 -depleted C2C12 cells. (prolekare.cz)
  • Prostate androgen regulated (PAR) protein is a 148 amino acid polypeptide ubiquitously expressed in normal cells and overexpressed in many malignancies. (biomedsearch.com)
  • The BRCT domain consists of approximately 95 amino acid residues and occurs as a tandem repeat at the carboxyl terminus of numerous proteins, but has been observed also as a tandem repeat at the amino terminus or as a single copy. (epfl.ch)
  • Azidohomoalaine (AHA) is a non-radioactive and "clickable" amino acid analog of methionine which can be incorporated into newly synthesized proteins. (bio-protocol.org)
  • The kinase most closely related to STK-1 is Xenopus laevis XLP46 protein kinase which shows 71% amino-acid identity to STK-1 between their kinase domains. (semanticscholar.org)
  • One of these brands, the acetylation of the amino acid lysine 16 of histone H4 protein (H4K16Ac), appears to be particularly important in regulating the organization and genome integrity. (technologynetworks.com)
  • Santa Cruz Biotechnology offers conjugated and unconjugated monoclonal primary antibodies directed against most cell cycle proteins. (scbt.com)
  • Immunofluorescence studies with protein phosphatase-1 (PP1) isoforms-specific antibodies detected PP1δ, but not α or γ1, at focal adhesions. (biochemj.org)
  • A ) Comparison of immunofluorescence stainings using several antibodies against ORF1p and HeLa M2 cells expressing a recoded L1. (elifesciences.org)
  • B ) Quantification of nuclear and cytoplasmic ORF1p and ORF2p in HeLa cells expressing a recoded L1 (ORFeus) or a native L1 (L1rp) and stained with JH74 or JH73g antibodies in combination with mouse anti-FLAG M2 antibody. (elifesciences.org)
  • C ) Western blot of HeLa M2 cells expressing ORFeus, L1rp or no L1 (HeLa) blotted with the indicated antibodies in combination with 4H1 always in green. (elifesciences.org)
  • Cells expressing ORFeus are fixed with formalin and stained with 4H1 mouse anti-ORF1p (red) and rabbit anti-FLAG antibodies (green). (elifesciences.org)
  • A ) HeLa-M2 cells transfected with empty pCEP-puro vector (LD207), L1rp (MT302) and ORFeus (LD401) were stained with secondary antibodies without incubation with primary antibodies. (elifesciences.org)
  • The Proliferating Cell Nuclear Antigen (PCNA) was initially found as a nuclear protein that was recognized by auto antibodies in patients with systemic lupus erythematosus and whose synthesis was related with a proliferative state of the cells since it was synthesized during the S phase of the cell cycle. (scielo.org.mx)
  • Certain 17β-estradiol enzymes transfer ubiquitin directly to the protein substrate, whereas others require the participation of a third component, termed a ubiquitin ligase (E3), to achieve this effect. (aacrjournals.org)
  • They focused on the role of proteins called cryptochromes, which are believed to have evolved from light-activated DNA repair enzymes in bacteria. (technologynetworks.com)
  • To investigate the potential role of these enzymes as anticancer drug targets, we have synthesized novel benzylidene-thiazolidine-2,4-diones that function as potent Pim protein kinase inhibitors. (elsevier.com)
  • In a study published in Cell , Mikhail Savitski - together with the Bork and Beck labs at EMBL Heidelberg - systematically studied the amount of different proteins during six phases of the cell cycle. (embl.org)
  • Each monomer contains a region called InterDomain Connector Loop (IDCL) which is bound by different proteins possessing a domain known as of Interaction to PCNA or PIP. (scielo.org.mx)
  • f ) The rate of Skp2 ubiquitination in vivo was measured in the presence or absence of pRB by transfecting HA-tagged ubiquitin and Flag-tagged Skp2 into SAOS-tetRB cells. (nature.com)
  • RBP45 and RBP33 are phosphoproteins, and RBP45 has been found to bind in vivo specifically to target mRNA containing cycling sequences. (asm.org)
  • These include a family of RNA binding proteins recently identified from Trypanosoma cruzi that shows highly restrictive binding interactions in vivo with specific mRNAs ( 9 ). (asm.org)
  • In vivo, Vpr might, by preventing p34cdc2 activation, delay or prevent apoptosis of infected cells. (asm.org)
  • Here, we report that these in vivo findings are consistent with observations made in cancer cells in culture. (aacrjournals.org)
  • An analysis of the c-myc protein in vivo shows that de novo synthesis occurs in G1 and G2 and the protein turns over with a half-life of approximately 20-30 min in both phases. (ox.ac.uk)
  • Thus, the presence of a nonfunctional Dbf2 protein, rather than the lack of function per se, is inhibitory. (genetics.org)
  • Fan, LM , Vinoj, G , Brooks, G and Li, JM (2008) Nox2 modulation of cell cycle inhibitory protein p21cip1 in endothelial cells In: Annual Scientific Conference of the British-Cardiovascular-Society/British-Society-for-Cardiovascular-Research, 2008-06-02 - 2008-06-04, Manchester, ENGLAND. (surrey.ac.uk)
  • The retinal pigment epithelial cells (RPE) play a pivotal role in the pathogenesis of the proliferative vitreoretinopathy (PVR). (arvojournals.org)
  • Transforming growth factor-β [TGF-β] is such an example, being a growth stimulator in fibroblastic cells with TGF-β receptors, but a negative regulator in epithelial cells. (biomedcentral.com)