• Biological and molecular responses of NB cells to epigenetic drugs and interferon (IFN)-γ exposure were assessed by proliferation assays, immunoblotting, ELISA, qRT-PCR, RNA-seq and ChIP-qPCR as well as co-culture assays with T cells. (bvsalud.org)
  • A vast amount of research exists on the possible molecular mechanisms through which vitamin D affects cancer cell proliferation, cancer progression, angiogenesis, and inflammation. (mdpi.com)
  • Reishi inhibits proliferation and induces programmed cell death in human prostate cancer cells. (greenmedinfo.com)
  • Ganoderma lucidum inhibits proliferation and induces apoptosis in human prostate cancer cells PC-3. (greenmedinfo.com)
  • In this study, we investigated the effects of Ganoderma lucidum on cell proliferation, cell cycle, and apoptosis in human prostate cancer cells PC-3. (greenmedinfo.com)
  • Our data demonstrate that Ganoderma lucidum inhibits cell proliferation in a dose- and time-dependent manner by the down-regulation of expression of cyclin B and Cdc2 and by the up-regulation of p21 expression. (greenmedinfo.com)
  • indicated that ATMs colocalized with T cells in lymphoid clusters within adipose tissue and may act as APCs, which express high levels of MHCII and also costimulatory molecules and process and present antigens to induce CD4+ T-cell proliferation and activation in adipose tissue of obese mice (29, 68, 105). (exposed-skin-care.net)
  • showed that adipose tissue from obese mice induced proliferation of splenic CD8+ T cells, indicating a CD8+ T cell-activating environment in obese adipose tissue (31). (exposed-skin-care.net)
  • Deletions of multiple GID subunits compromise cell proliferation, and this defect is accompanied by deregulation of critical cell cycle markers such as the retinoblastoma (Rb) tumor suppressor, phospho-Histone H3 and Cyclin A. We identify the negative regulator of pro-proliferative genes Hbp1 as a bonafide GID/CTLH proteolytic substrate. (elifesciences.org)
  • MTT assays were employed to determine the effect of cell proliferation. (biomedcentral.com)
  • Meanwhile, overexpression of ENDOD1 suppressed cell proliferation, induced G0/G1 cell cycle arrest and inhibited cell migration and invasion. (biomedcentral.com)
  • Conversely, siRNA-mediated silencing of ENDOD1 promoted cell proliferation, migration and invasion. (biomedcentral.com)
  • Alanine transition of S63 and S262 (S63A and S262A) of IκBα induced inhibition of cell proliferation and suppression of p65 transcription activity. (researchsquare.com)
  • In addition, cell proliferation and angiogenesis will be stimulated, enabling better oxygenation of the tissue. (bmj.com)
  • We also showed that dose-dependent induction of proliferation was connected with changes in the expression of MKI67, CCND1 and CCNE1 genes in well- and poorly differentiated cancer cells. (spandidos-publications.com)
  • Our study revealed that Ang II influences EC cells in terms of cancer-related processes, and is responsible for increased proliferation, reduction in apoptosis, increased mobility and modulation of adhesion potential. (spandidos-publications.com)
  • Therefore, various biologically active peptides such as angiotensin, affecting cell proliferation, have become a new area of study in endometrial cancer research. (spandidos-publications.com)
  • Recently, a number of studies have shown that Ang II plays an important role in proliferation, invasiveness and migration of tumour cells, alteration of expression of cancer-related genes, as well as in physiological tissue remodelling. (spandidos-publications.com)
  • Effects of Ganoderma lucidum spores on HepG2 cells proliferation and growth cycle. (springer.com)
  • To investigate the effects of silibinin on the growth of oral cancer cells, cell proliferation and anchorage-independent colony formation tests were conducted on YD10B and Ca9-22 oral cancer cells. (jcancer.org)
  • Silibinin effectively suppressed YD10B and Ca9-22 cell proliferation and colony formation in a dose-dependent manner. (jcancer.org)
  • Cell proliferation was reduced in a time-dependent manner of drug exposure as revealed from BrdU incorporation and migration studies. (jcimjournal.com)
  • Effects of Thuja occidentalis ethanolic extract on cellular proliferation tested by BrdU incorporation assay Cells were exposed to three different concentration of Thuja occidentalis ethanolic extract for 6, 12 and 24 h along with incorporation of BrdU. (jcimjournal.com)
  • It does not have the morphological characteristics of typical necrosis, such as swelling of the cytoplasm and organelles and rupture of the cell membrane, nor does it have the characteristics of traditional cell apoptosis, such as cell shrinkage, chromatin condensation, formation of apoptotic bodies and disintegration of the cytoskeleton. (nature.com)
  • Evidences are rising about the ability of some copper compounds to induce alternative non-apoptotic form of programmed cell death. (gla.ac.uk)
  • Our data suggest that 125 I seeds radiation can induce paraptosis-like cell death via PI3K/AKT signaling pathway. (hindawi.com)
  • In a preliminary study it has been shown that phenolic antioxidants such as methyl 4- O -galloylchlorogenate and 4- O -galloylchlorogenic acid derived from Sapota fruits can induce cytotoxicity in colon cancer cells 23 . (nature.com)
  • Indeed, the principal adipocytes isolated from obese mice could induce antigen-specific Compact disc4+ T cell activation (58). (exposed-skin-care.net)
  • Furthermore, Chaga has been found to induce autophagy via the AMPK-mTOR signaling pathway in breast cancer cells, suggesting that it may have potential as an adjuvant therapy for cancer treatment. (chagamushrooms.net)
  • Low ATP concentrations in hypoxic tumour cells disable the apoptotic cascade and induce cell death by necrosis. (bmj.com)
  • Ganoderic acid Mf and S induce mitochondria mediated apoptosis in human cervical carcinoma HeLa cells. (springer.com)
  • Conclusion: Prion protein 106-126 peptide can induce apoptosis in differentiated PC12 cells and present cellular toxicity definitely. (researchgate.net)
  • A Canadian study, for example, has observed both in vitro and in vivo how the stem differentiation factors are able to induce apoptosis in tumor cells. (biava.me)
  • On the other side, many copper complexes were synthesized and tested for their anticancer activity in vitro and in vivo. (gla.ac.uk)
  • Capsid gene delivery into the striatum of mouse brain or interskeletal muscle resulted in cell death and inflammation, likely through capsid-induced apoptosis in vivo. (cdc.gov)
  • Using PCa cellular models in vitro and in vivo we show that MCL1 expression is upregulated in androgen-deprived PCa cells. (oncotarget.com)
  • We aimed to examine the molecular processes underlying the effects of silibinin in oral cancer in vitro and in vivo as well as its potential anticancer effects. (jcancer.org)
  • Next, we investigated the molecular processes underlying both in vitro and in vivo outcomes of silibinin treatment on oral cancer. (jcancer.org)
  • A.J. McGahon, S.J. Martin, R.P. Bissonnette, A. Mahboubi, Y. Shi, R.J. Mogil, W.K. Nishioka, and D.R. Green, The End of the (Cell) Line: Methods for the Study of Apoptosis In Vitro. (nhbs.com)
  • Androgen deprivation therapy induces apoptosis or cell cycle arrest in prostate cancer (PCa) cells. (oncotarget.com)
  • The BH3-mimetic Obatoclax induces apoptosis and decreases MCL1 expression in androgen-sensitive PCa cells, while castration-resistant PCa cells are less sensitive and react with an upregulation of MCL1 expression. (oncotarget.com)
  • AuNPs interact with cell wall macromolecules, damaging them and affecting membrane proteins [ 9 ] . (encyclopedia.pub)
  • Unlike necrosis, an unregulated form of cell death caused by external physicochemical stress, necroptosis is highly regulated and acts as a defense mechanism or escape route for cells infected with viruses that inhibit apoptosis signaling proteins (examples of these viruses include the murine cytomegalovirus, West Nile virus, human immunodeficiency virus-1 (HIV-1), and herpes simplex virus). (bioradiations.com)
  • Apoptosis, on the other hand, is characterized by cell shrinkage, membrane blebbing, condensation, margination of nuclear chromatin, packaging of apoptotic bodies, and engulfment of the immunogenic intracellular proteins by phagocytes and macrophages. (bioradiations.com)
  • Cellular proteostasis involves the coordinated and compensatory action of pathways that control biogenesis, folding, trafficking and breakdown of proteins allowing the cell to adapt to physiological or pathological environmental changes. (elifesciences.org)
  • Khz reduced MMP and Bcl-2 protein levels, but increased ROS generation and p53 and pro-apoptotic proteins. (springer.com)
  • To address this issue, primary mouse cells, haploinsufficient for one or two proteins, ATM and RAD9, related to the cellular response to DNA damage were examined. (aacrjournals.org)
  • The results show that cells having low levels of both ATM and RAD9 proteins are more sensitive to transformation by radiation, have different DNA double-strand break repair dynamics and are less apoptotic when compared with wild-type controls or those cells haploinsufficient for only one of these proteins. (aacrjournals.org)
  • Our conclusions are that under stress conditions, the efficiency and capacity for DNA repair mediated by the ATM/RAD9 cell signaling network depend on the abundance of both proteins and that, in general, DNA repair network efficiencies are genotype-dependent and can vary within a specific range. (aacrjournals.org)
  • Another conclusion from most of the cases is that animals or cells haploinsufficient for the specified proteins have higher transformation rates after DNA damage is induced, but when their DNA is not significantly damaged by exogenous sources, tumor development rates are the same as for their wild-type counterparts. (aacrjournals.org)
  • Furthermore, silibinin inhibited the migration and invasion abilities of YD10B and Ca9-22 cells by regulating the expression of proteins involved in the epithelial-mesenchymal transition. (jcancer.org)
  • This similarity between tumor and stem cells is as much strong that tumor cells activate receptors responding to the same proteins which effect embryonic stem cells and have the task of transforming initial informal mass of embryonic cells into different tissues that constitute the body. (biava.me)
  • Our results indicate that several dozens of proteins for each cell type, are PE-lipoxidated in HT-22, MLE, and H9c2 cells and M2 macrophages after they were induced to undergo ferroptosis. (cdc.gov)
  • Pretreatment of cells with the strong nucleophile, 2-mercaptoethanol, prevented the formation of PE-lipoxidated proteins and blocked ferroptotic death. (cdc.gov)
  • It has generally been described that the toxic effects of copper complexes leads to cell death either by necrosis or through the activation of the apoptotic process. (gla.ac.uk)
  • Annexin V-propidium iodide double-staining demonstrated that Sapota fruit extracts potentiate apoptosis rather than necrosis in cancer cells. (nature.com)
  • Traditionally, cell death has been divided into apoptosis and necrosis. (nature.com)
  • Recent studies have shown that in addition to necrosis and apoptosis, there are also other new programmed death modes, such as autophagy, necrosis and necrotic apoptosis, which have unique biological processes and pathophysiological characteristics. (nature.com)
  • Their ability to kill cancer cells is mainly related to the induction of an oxidative stress, but recently it emerged their ability to inhibit the proteasome, a protein complex whose proteolitic activity is needed by several cellular process. (gla.ac.uk)
  • L.T. Bemis, F.J. Geske, and R. Strange, Use of the Yeast Two-Hybrid System for Identifying the Cascade of Protein Interactions Resulting in Apoptotic Cell Death. (nhbs.com)
  • From development to aging and disease, the brain parenchyma is under the constant threat of debris accumulation, in the form of dead cells and protein aggregates. (frontiersin.org)
  • Mitochondria not only serve as power plants in cells but also act as crucial regulators in many biological processes, including reactive oxygen species (ROS) signaling, redox balance, calcium homeostasis, protein quality control, and programmed cell death ( 3 , 4 ). (frontiersin.org)
  • Necroptosis is a programmed form of necrotic cell death that is dependent on activation of receptor interacting protein kinase-3 (RIPK3) and occurs when caspase activation is insufficient or blocked. (bioradiations.com)
  • Under conditions that sensitize the cell to death, a cell death-inducing complex of RIPK1, Fas-associated protein via death domain (FADD), caspase-8, and cellular FLICE-like inhibitory protein (cFLIP) is formed. (bioradiations.com)
  • Real-time PCR and western blot were used to detect ENDOD1 mRNA and protein expression in normal prostatic epithelial and PCa cell lines. (biomedcentral.com)
  • Western blot showed that androgen-sensitive LNCaP cells had the highest protein expression level of ENDOD1, whereas castration-resistant PCa cell lines PC3 and DU145 had significantly lower protein levels. (biomedcentral.com)
  • Background: The phosphorylation of NF-kappaB inhibitor alpha (IκBα) protein is pivotal to the regulation of NF-κB transcription factor activity in the cell. (researchsquare.com)
  • Most of the important molecular processes in the cell, such as DNA replication, are performed by large molecular network of protein-protein interactions. (researchsquare.com)
  • These studies demonstrate that the capsid protein of WNV may be responsible for aspects of viral pathogenesis through induction of the apoptotic cascade. (cdc.gov)
  • Effects of triterpenes from Ganoderma lucidum on protein expression profile of HeLa cells. (springer.com)
  • the expression levels of protein associated with apoptosis and endoplasmic reticulum stress (ERS) of SKO-007 cells in various groups were detected by Western blotting method. (researchgate.net)
  • Objective: To investigate the effects of prion protein 106-126 peptide on inducing apoptosis in differentiated PC12 cells. (researchgate.net)
  • Methods: After differentiated by nerve growth factor (NGF), the PC12 cells were infected by prion protein 106-126 peptide. (researchgate.net)
  • Analysis of MCL1 protein and mRNA expression in PCa tissue and primary cell culture specimens of luminal and basal origin, respectively, reveals higher expression in cancerous tissue compared to benign origin. (oncotarget.com)
  • The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. (cancerindex.org)
  • An image depicting head and neck squamous cell carcinoma in vitro can be seen below. (medscape.com)
  • Head and neck squamous cell carcinoma in vitro (cell culture). (medscape.com)
  • Cancer Lett, 300:215-224.doi:10.1016/j. squamous cell carcinoma in north-eastern Iran. (who.int)
  • Khz cytotoxicity was measured using an MTT assay and the mitochondrial membrane potential (MMP)-related, calcium-induced generation of reactive oxygen species (ROS) in A549 cells was measured by flow cytometry. (springer.com)
  • EHMT and EZH2 inhibitor response gene signatures from NB cells were established as surrogate measures and revealed high EHMT and EZH2 activity in MYCN-amplified high-risk NBs with a cold immune phenotype. (bvsalud.org)
  • Moreover, inhibitor of PI3K/AKT signaling pathway could inhibit paraptosis-like cell death induced by 125 I seeds radiation. (hindawi.com)
  • And paraptosis is also insensitive to apoptotic and autophagic inhibitor [ 10 ]. (hindawi.com)
  • A hypoxic environment devoid of nutrients prevents the cell undergoing energy dependent apoptosis and cells become necrotic. (bmj.com)
  • Because of breakthroughs such as the "switching" mechanism of the p53 tumor suppressor gene, as well as many technical innovations, the number of investigators of cell death has rapidly expanded over the last few years. (nhbs.com)
  • p53, a tumor suppressor gene, helps regulate the cell cycle. (biomedcentral.com)
  • These two important mechanisms of approaching cancer, principally through the clinical application of the P53 tumor suppressor gene and other apoptotic players that for the past 15 years have been included in my cancer strategy as a diagnostic, prognosis, and follow up treatment. (biobran.org)
  • Notably, tumors expressing LCMV-gp33 as a model antigen are sensitized to gp33-TCR transgenic T cell-mediated cytotoxicity upon PGRN blockade. (bvsalud.org)
  • Extraction from 9 parameters of KSO-SFE was screened for cytotoxicity towards human colorectal cancer cell lines (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines using MTS assay. (hindawi.com)
  • Therefore, this study determined the cytotoxicity of KSO-SFE on colon cancer cells. (hindawi.com)
  • Here we show that methanolic extracts of Sapota fruit (MESF) induces cytotoxicity in a dose-dependent manner in cancer cell lines. (nature.com)
  • In vitro studies have demonstrated that Chaga extracts and small phenolic compounds derived from the mushroom exhibit cytotoxicity against various cancer cell lines. (chagamushrooms.net)
  • Miyajima, A, Nakashima J, Yoshioka K. Role of reactive oxygen species in cis-dichlorodiammineplatium-induced cytotoxicity on bladder cancer cells. (springer.com)
  • Cell Death provides a current and comprehensive collection of methods for the study of cell death. (nhbs.com)
  • In the tradition of this long-standing series, this volume's user-friendly recipes and more than 100 tables and figures make the study of cell death accessible across life science disciplines. (nhbs.com)
  • Students of apoptosis and other types of cell death hypothesize that virtually all cells are genetically programmed to self-destruct. (nhbs.com)
  • In turn, they also have learned that massive cell death does not always represent pathology, but rather a normal and necessary "weeding out" in the development and life of an organism. (nhbs.com)
  • It offers a cross-disciplinary look at cell death using a broad range of technical approaches and model systems. (nhbs.com)
  • The techniques described facilitate both understanding and regulation of cell death, promising to have immense value in the clinical treatment of cancer. (nhbs.com)
  • J.F.R. Kerr, G.C. Gobe, C.M. Winterford, and B.V. Harmon, Anatomical Methods in Cell Death. (nhbs.com)
  • B.A. Osborne, S.W. Smith, Z.-G. Liu, K.A. McLaughlin, and L.M. Schwartz, Transient Transfection Assays to Examine the Requirement of Putative Cell Death Genes. (nhbs.com)
  • DNA fragmentation was first documented by Williamson in 1970 when he observed discrete oligomeric fragments occurring during cell death in primary neonatal liver cultures. (wikipedia.org)
  • Apoptotic DNA fragmentation is a natural fragmentation that cells perform in apoptosis (programmed cell death). (wikipedia.org)
  • Many studies on the cancer-killing activity of 125 I seeds radiation mainly focused on its ability to trigger apoptosis, which is the most well-known and dominant type of cell death induced by radiation. (hindawi.com)
  • We found that 125 I seeds radiation activated PI3K/AKT signaling pathway and could trigger paraptosis-like cell death. (hindawi.com)
  • The work presented here characterizes a novel form of cell death in response to 125 I seeds radiation. (hindawi.com)
  • Paraptosis is a kind of caspase-independent programmed cell death and is characterized by distinct cytoplasmic vacuolization derived from swelling endoplasmic reticulum and/or mitochondria. (hindawi.com)
  • This form of cell death is fundamentally different from apoptosis and lacks some distinct characteristics of apoptosis such as DNA fragmentation, pyknosis, or caspase activation and cleavage [ 14 ]. (hindawi.com)
  • Necroptosis is often compared to apoptosis, another programmed cell death pathway. (bioradiations.com)
  • The two cell death pathways share several upstream signaling elements and eventually lead to plasma membrane rupture, but the cell morphologies of each process are drastically different. (bioradiations.com)
  • This leads to a series of auto- and cross-phosphorylations between RIPK1 and RIPK3 that lead to formation and activation of a cell death-inducing complex called a necrosome. (bioradiations.com)
  • 3- 5 Therefore, during severe hypoxia or anoxia, the cell initiates a cascade of events that leads to apoptotic cell death, thereby preventing the accumulation of cells with hypoxia induced mutations. (bmj.com)
  • 7 Severe hypoxia in the presence of energy stimulates cells to undergo apoptosis, whereas oxygen levels above 0.5% prevent cell death. (bmj.com)
  • We report that the WNV capsid (Cp) by itself induces rapid nuclear condensation and cell death in tissue culture. (cdc.gov)
  • Programmed cell death and Bcl-2 protection in very low oxygen. (springer.com)
  • Jacobson MD. Reactive oxygen species and programmed cell death. (springer.com)
  • Heparin prevents programmed cell death in human trophoblast. (mdx.ac.uk)
  • Apoptosis is a genetically controlled cell death that was initially recognized for its role in development. (jneurosci.org)
  • Ferroptosis-inducing factors can directly or indirectly affect glutathione peroxidase through different pathways, resulting in a decrease in antioxidant capacity and accumulation of lipid reactive oxygen species (ROS) in cells, ultimately leading to oxidative cell death. (nature.com)
  • Ferroptosis is a new type of programmed cell death, which occurs with iron dependence. (nature.com)
  • What is the relationship between ferroptosis and other types of cell death? (nature.com)
  • Whether under physiological or pathological conditions, cell death is an unavoidable and important link in the process of life and marks the end of the life of a cell. (nature.com)
  • In 2012, Dixon 1 first proposed the concept of ferroptosis, an iron-dependent, non-apoptotic mode of cell death characterized by the accumulation of lipid reactive oxygen species (ROS). (nature.com)
  • In the meantime Biava and colleagues' have continued with their research which at the end allowed to identify various molecular mechanisms employed by the differentiation factors of normal stem cells, taken from the embryo of Zebrafish (chosen due to its being the most studied model of embryonic differentiation), to differentiate tumor cells or lead them to a programmed cell death. (biava.me)
  • Ferroptosis is an iron dependent form of cell death, that is triggered by the discoordination of iron, lipids, and thiols. (cdc.gov)
  • Its unique signature that distinguishes it from other forms of cell death is the formation and accumulation of lipid hydroperoxides, particularly oxidized forms of polyunsaturated phosphatidylethanolamines (PEs), which drives cell death. (cdc.gov)
  • 2001. Subchronic dermal application of N,N-diethyl m-toluamide (DEET) and permethrin to adult rats, alone or in combination, causes diffuse neuronal cell death and cytoskeletal abnormalities in the cerebral cortex and the hippocampus, and purkinje neuron loss in the cerebellum. (cdc.gov)
  • However, the molecular mechanisms underlying such age-dependent differences in apoptotic response to neuronal injury have not been identified. (jneurosci.org)
  • Eldadah and Faden, 2000 ), we hypothesized that suppression of apoptotic capability during maturation of mammalian brain results from repression of genes involved in the caspase-3 activation pathway, and that injury-induced neuronal apoptosis in the mature brain results from reactivation of these genes. (jneurosci.org)
  • Western Blot and TUNEL results suggested that Nigella sativa seed extracts regulated the expression of pro- and anti- apoptotic genes, indicating its possible development as a potential therapeutic agent for cervical cancer upon further investigation. (biomedcentral.com)
  • Cells will adapt by activating the expression of genes involved in metabolic adaptation, such as those involved in glycolysis. (bmj.com)
  • After Ang II treatment, poorly differentiated endometrial cancer cell line acquired a mesenchymal phenotype, which was characterized by induced expression of EMT-related genes (VIM, CD44, SNAI1, ZEB1 and ZEB2). (spandidos-publications.com)
  • Moreover, we examined alterations in the expression of genes related to cancer cell behaviour after Ang II treatment. (spandidos-publications.com)
  • These results suggest that differential expression of Apaf-1 and caspase-3 genes may underlie regulation of apoptotic susceptibility during brain development, as well as after acute injury to mature brain, through the intrinsic pathway of caspase activation. (jneurosci.org)
  • Method: Anti-proliferative and apoptotic effect of various doses of essential oil from Ferula asafoetida was investigated on colon cancer cell lines i.e. (ac.ir)
  • Result: The potent anti-proliferative activity has been observed in both the cell lines. (ac.ir)
  • This study aimed to investigate the anti-proliferative effects of Khz on A549 lung cancer cells. (springer.com)
  • The anti-proliferative and pro-apoptotic effects of Khz suggest that this extract shows great promise as a potential chemotherapeutic agent. (springer.com)
  • Caspases, a class of cysteine proteases, are central players in the apoptotic process that trigger a cascade of proteolytic cleavage events [ 14 ]. (biomedcentral.com)
  • Apoptosis an active physiological process resulting in cellular self-destruction of unwanted cells is absent in cancer cells. (biomedcentral.com)
  • Necroptosis is characterized by increased cell volume, swelling of organelles, perforation of the plasma membrane, cellular collapse, release of cellular contents that triggers an innate and adaptive immune response, and clearance of the necroptotic cells by macropinosomes. (bioradiations.com)
  • Macropinosomes, a subcellular component of macrophages, clear the necroptotic cells through pinocytosis, a process of "cellular drinking" in which the broken-down cells are brought into the macropinosome and suspended within small vesicles that fuse with lysosymes to hydrolyze the cell. (bioradiations.com)
  • Our biochemical and cellular analysis thus demonstrates that the GID/CTLH complex prevents cell cycle exit in G1, at least in part by degrading Hbp1. (elifesciences.org)
  • This is the multihit theory of tumorigenesis, in which a series of multiple triggering events in the genetic and cellular makeup of a cell ultimately cause cancer. (medscape.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • This clinical cancer strategy incorporates advancements in cancer treatment based on a multi-approach to the disease that includes targeting apoptosis, immune cell activation, angiogenesis, restoration of cellular respiration, inhibition of inflammatory mediators, not mentioning some secondary factors such as intoxication, disturbed microbiome, and nervous disorders. (biobran.org)
  • The degree of DNA fragmentation in sperm cells can predict outcomes for in vitro fertilization (IVF) and its expansion intracytoplasmic sperm injection (ICSI). (wikipedia.org)
  • Processed hepatic evaluating program within chronic graft-versus-host condition enhances prognostic danger stratification associated with long-term outcomes. (bcl-2protein.com)
  • We found besides apoptosis that 125 I seed radiation killed colorectal cancer cell via inducing paraptosis. (hindawi.com)
  • We will then describe that resulting from engulfment and degradation of apoptotic cargo, phagocytes undergo an epigenetic, transcriptional and metabolic rewiring that leads to trained immunity, and discuss its relevance for microglia and brain function. (frontiersin.org)
  • In MYCN-amplified NB cells, co-inhibition of EZH2 (enhancer of zeste homologue 2), a H3K27 histone methyltransferase cooperating with EHMTs, was needed for strong transcriptional responses to IFN-γ, in line with histone mark changes at CXCL9 and CXCL10 chemokine gene loci. (bvsalud.org)
  • Biological assays and gene expression analysis were performed on three cell lines: ISH, MFE-296 and MFE-280. (spandidos-publications.com)
  • Here we demonstrate that, during brain maturation, the potential of the intrinsic apoptotic pathway is progressively reduced and that such repression is associated with downregulation of apoptotic protease-activating factor-1 (Apaf-1) and caspase-3 gene expression. (jneurosci.org)
  • Treatment of CRC cells with MS-444 resulted in growth inhibition and increased apoptotic gene expression, while similar treatment doses in non-transformed intestinal cells had no appreciable effects. (oncotarget.com)
  • Transwell migration and invasion assays were done to evaluate changes in the ability of cell migration and invasion. (biomedcentral.com)
  • Biological assays were performed on well-[Ishikawa (ISH)] moderately (MFE-296) and poorly (MFE-280) differentiated human adenocarcinoma cancer cell lines, representing the G1, G2 and G3 stage of EC. (spandidos-publications.com)
  • The effects of silibinin on the migration and invasion of oral cancer cells were evaluated using transwell assays. (jcancer.org)
  • Effects of Thuja occidentalis ethanolic extract on A549 cell migration (Light microscopy, ×20) Wound-healing assays were performed to assess cell migration. (jcimjournal.com)
  • Studies have shown that cells can eliminate defective mitochondria by delivering them to recipient cells (such as macrophages) to maintain homeostasis. (frontiersin.org)
  • Another statement showed the preadipocyte- and endothelial cell-derived stromal-derived element-1 (CXCL12), mediated early infiltration of CD4+ T lymphocytes in obesity, which preceded the increase of macrophages in adipose cells of mice on HFD (101). (exposed-skin-care.net)
  • Interestingly, cytokines from type-2 innate immune cells, such as M2 macrophages or D2 dendritic cells exhibit a protective effect on both types of diabetes. (biobran.org)
  • Myeloid immune cells, specifically tumor-associated macrophages (TAMs), may represent potential novel therapeutic targets in HCC, complementing current ablative or immune therapies. (cancerindex.org)
  • During apoptosis, the apoptotic effector caspase, caspase 3, cleaves ICAD and thus causes CAD to become activated. (wikipedia.org)
  • Similar to CD4+ T cells, CD8+ T cells exhibit effector memory or effector phenotypes expressing elevated levels of IFN- in obese adipose tissue (31, 44). (exposed-skin-care.net)
  • Inhibition of PGRN abrogates autophagy-dependent MHCI degradation and restores MHCI expression on PDAC cells. (bvsalud.org)
  • We further focused our interest on the molecular mechanisms that underlie 125 I seeds radiation-induced paraptosis on colorectal cancer cells. (hindawi.com)
  • Then, we will discuss several principles that govern macrophage phagocytosis of apoptotic cells (efferocytosis), including the existence of redundant recognition mechanisms ("find-me" and "eat-me") that lead to a tight coupling between apoptosis and phagocytosis. (frontiersin.org)
  • Thus, Ganoderma lucidum exerts its effect on cancer cells by multiple mechanisms and may have potential therapeutic use for the prevention and treatment of cancer. (greenmedinfo.com)
  • [ 6 ] These events lead the cancer cell to escape normal cell growth and control mechanisms, to avoid system control mechanisms (ie, immunologic surveillance), and to establish a nutrient supply. (medscape.com)
  • How to intervene in the occurrence and development of related diseases by regulating cell ferroptosis has become a hotspot and focus of etiological research and treatment, but the functional changes and specific molecular mechanisms of ferroptosis still need to be further explored. (nature.com)
  • Cell cycle analysis showed a significant increase in the accumulation of KSO-SFE-treated cells at sub-G1 phase, indicating the induction of apoptosis by KSO-SFE. (hindawi.com)
  • Premedicate Kyprolis infusions with dexamethasone prior to all Cycle 1 doses and if infusion reaction symptoms develop or reappear. (aetna.com)
  • Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. (affbiotech.com)
  • Cell viability was ascertained through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay after deployment of TO in different doses. (jcimjournal.com)
  • Insulin-like growth factor-II and heparin are anti-apoptotic survival factors in human villous cytotrophoblast. (mdx.ac.uk)
  • RD cells transfected with pcWNV-Cp-DJY or pcDNA3.1 sis in vitro through the mitochodrial/caspase-9 pathway. (cdc.gov)
  • The proven essentiality of copper for angiogenesis, together with the marked sensitivity shown by several cancer cell lines to the copper toxicity, open a new perspective in the anticancer strategy: exploiting the tumor need of copper to accumulate toxic amount of the metal inside its cells. (gla.ac.uk)
  • To study the possible anticancer and antiproliferative activities of ethanolic leaf extract of Thuja occidentalis (TO) on A549 non-small lung carcinoma cells in vitro . (jcimjournal.com)
  • They demonstrated that transferring functional mitochondria of bone marrow-derived stem cells to defective parenchymal cells increases the aerobic respiration capacity of recipient mitochondria ( 7 ). (frontiersin.org)
  • Cancer stem cells are responsible for tumor chemoresistance and recurrence in adjuvant and metastatic settings. (isciii.es)
  • The objective of this article was to evaluate how these chemotherapeutic regimens affect the proportion of cancer stem cells and the expression of stemness markers. (isciii.es)
  • After analyzing different tumor stem cell markers (SOX2, OCT4, CD133, CD44 and CD24) in pancreatic cancer cells treated with different chemotherapeutic protocols by means of RT-qPCR, Oxaliplatin and Gemcitabine in monotherapy were the chemotherapies that selected the most cancer stem cells while the FOLFIRI protocol decreased them. (isciii.es)
  • Recurrence and chemoresistance in pancreatic cancer are largely related to cancer stem cells. (isciii.es)
  • The aim of this study was to determinate the effect of different chemotherapy protocols used in the clinic on tumor stem cell markers in vitro . (isciii.es)
  • Exosomes (EXOs) produced by stem cells have grown to be a potential brand new answer to intense myocardial infarction (AMI). (bcl-2protein.com)
  • Objective: To investigate the induction of tanshinone [1 A (Tan fl A) on the differentiation of human placenta-derived mesenchymal stem cells (hPDMSCs) into cardiomyocytes, and to provide an experimental basis for Tan IT A as a cardiomyocytc differentiation inducer. (researchgate.net)
  • Intelligent Information gives birth to Life: it is the factors existing at 5 stages of stem cells differentiation that determine the fate of healthy and pathological cells. (biava.me)
  • These are the results attained by a partnership of 23 Italian universities aimed at the study of new frontiers of cells reprogramming in order to determine fate of healthy and pathological stem cells. (biava.me)
  • It took almost 20 years to frame these observations until "Nature", one of the most prestigious scientific journals in the world, published an article in 2007, where it was shown that cancer cells behave in a very similar way to stem cells and in particular those of zebrafish were referred to in the study. (biava.me)
  • a cancer cell is a cell that returns to its archaic state , i.e. when it was a stem cell, and it behaves as such. (biava.me)
  • The theoretical basis for marrow failure includes primary defects in or damage to the stem cell or the marrow microenvironment. (medscape.com)
  • External insults (eg, infections, radiation, drugs) may disrupt stem cell homeostasis in marrow environment, leading to altered growth. (medscape.com)
  • S.A. Ben-Sasson, Y. Sherman, and Y. Gavrieli, Identification of Dying Cells--In Situ Staining. (nhbs.com)
  • Fluorescence-activated cell sorting analysis after annexin V-fluorescein isothiocyanate and propidium iodide (annexin V-FITC-PI) dual staining method was done to ascertain early stage of apoptosis, if any. (jcimjournal.com)
  • In a first proof-of-concept, the developed molecules allow studies on isoform-selective effects of Akt inhibition in cancer cells. (bvsalud.org)
  • In the present study we investigated the efficacy of Organic extracts of Nigella sativa seed powder for its clonogenic inhibition and induction of apoptosis in HeLa cancer cell. (biomedcentral.com)
  • The inhibition of cell wall β-glucan synthase leads to changes in the cell wall integrity and further cell damage [ 9 ] [ 10 ] . (encyclopedia.pub)
  • The inhibition of cell growth was also demonstrated by cell cycle arrest at G2/M phase. (greenmedinfo.com)
  • such as the complicated I inhibition-independent improvement of energy and Operating-system exhaustion, probably through the destabilization of the MT program and obstruction of voltage-dependent anions stations (VDACs), leading 604-80-8 manufacture to cell-cycle interruptions, advertising of neuroprotection and difference, and the service of apoptotic paths. (scienceexhibitions.org)
  • We have previously demonstrated that Ganoderma lucidum down-regulated the expression of NF-kappaB-regulated urokinase plasminogen activator (uPA) and uPA receptor (uPAR), which resulted in suppression of cell migration of highly invasive human breast and prostate cancer cells. (greenmedinfo.com)
  • In cells stimulated to undergo ferroptosis we identified PE-truncated species with sn-2 truncations ranging from 5 to 9 carbons. (cdc.gov)
  • Regulation of MCL1 through the AR signaling axis is indirectly mediated via a cell cycle-dependent mechanism. (oncotarget.com)
  • Induction of apoptosis by ethanol extracts of Ganoderma lucidum in human gastric carcinoma cells. (springer.com)
  • Using constructs downregulating or overexpressing MCL1 we demonstrate that expression of MCL1 prevents induction of apoptosis when PCa cells are grown under steroid-deprived conditions. (oncotarget.com)
  • Mammalian cells have developed a range of adaptations to survive acute and even prolonged hypoxia. (bmj.com)
  • However, the main element substances that mediate T cell infiltration into adipose tissues in maturing remain to become discovered. (exposed-skin-care.net)
  • These brief peptide sequences in the antigen are often presented over the cell surface area of APCs by using MHCII substances, which are necessary for activation of Compact disc4+ T cells (102). (exposed-skin-care.net)
  • further defined that mostly huge adipocytes from obese adipose tissues exhibited an increased expression degree of MHCII substances and acted as APCs to activate Compact disc4+ T cells to secrete IFN- (103). (exposed-skin-care.net)
  • Therefore, a clearer understanding capsid expression was analyzed by immunofluorescent assay of WNV is necessary in order to identify new strategies to treat in HeLa, 293-T, RD, or SH-SY5Y cells by using anti-His tag or prevent this viral infection (3). (cdc.gov)
  • DVTP nick-end labeling (TUNEL) assay with the In Situ Cell determined according to the manufacturer's protocol. (cdc.gov)
  • Cell viability was determined by using the MTT assay and the morphological changes were observed. (researchgate.net)
  • Effects of Thuja occidentalis ethanolic extract on viability of A549 cell line (A) and L-132 cell line (B) Cells were exposed to Thuja occidentalis ethanolic extract for 24 h at different concentrations and the cell viability was determined by MTT assay. (jcimjournal.com)
  • METHODS: NB transcriptome analyses were used to identify epigenetic drivers of a T-cell infiltration-poor TME. (bvsalud.org)
  • RESULTS: We identified H3K9 euchromatic histone-lysine methyltransferases EHMT2 and EHMT1, also known as G9a and GLP, as epigenetic effectors of the MYCN-driven malignant phenotype and repressors of IFN-γ transcriptional responses in NB cells. (bvsalud.org)
  • CONCLUSION: Our results delineate a strategy for targeted epigenetic immunomodulation of high-risk NBs, whereby EHMT inhibitors alone or in combination with EZH2 inhibitors (in particular, MYCN-amplified NBs) could promote a T-cell-infiltrated TME via enhanced Th1-type chemokine expression. (bvsalud.org)
  • Recent animal studies also indicate that necroptosis may regulate the number of T cells in peripheral tissues and is required for clearance of abnormal lymphocytes during T cell development. (bioradiations.com)
  • In obese humans, adipocyte-secreted CCL20 may contribute to the deposition of Compact disc4+ helper and Compact disc8+ cytotoxic T lymphocytes within adipose tissues, possibly via connections with CCR6 which was upregulated on T cells in obese adipose tissues (100). (exposed-skin-care.net)
  • However our results showed some unique morphological features such as cell swelling, cytoplasmic vacuolation, and plasma membrane integrity, which is obviously different to apoptosis. (hindawi.com)
  • He described the cytoplasmic DNA isolated from mouse liver cells after culture as characterized by DNA fragments with a molecular weight consisting of multiples of 135 kDa. (wikipedia.org)
  • In pathological conditions, damaged cells transfer dysfunctional mitochondria toward recipient cells to ask for help and take up exogenous functional mitochondria to alleviate injury. (frontiersin.org)
  • Moreover, it induced cell cycle arrest in the G0/G1 phase, apoptosis, and ROS generation in these cells. (jcancer.org)
  • Silibinin considerably reduced the development of oral cancer cells by inducing apoptosis, G 0 /G 1 arrest, ROS generation, and activation of the JNK/c-Jun pathway. (jcancer.org)
  • Cancer is characterized by uncontrolled growth and division of a cell, with extension beyond the normally limiting basement membrane and through the boundaries of normal cells. (medscape.com)
  • Zhao S, Ye G, Fu G, Cheng JX, Yang BB, Peng C. Ganoderma lucidum exerts anti-tumor effects on ovarian cancer cells and enhances their sensitivity to cisplatin. (springer.com)
  • P.J. Fraker, L.E. King, D. Lill-Elghanian, and W.G. Telford, Quantification of Apoptotic Events in Pure and Heterogeneous Populations of Cells using the Flow Cytometer. (nhbs.com)
  • The cell populations doubling moments (PDT) demonstrated in Fig. 1B suggest that rotenone results on expansion change with period also. (scienceexhibitions.org)
  • The ISL IVR was found to be safe and well tolerated in the macaques with no observed mucosal cytokine changes or alterations in peripheral CD4 T-cell populations. (cdc.gov)
  • Contrarily, by implanting human tumor cells after the organogenesis is completed and therefore when the differentiating factors have decreased, tumor cells grow and replicate. (biava.me)
  • Previous study has shown that kenaf seed oil extracted by SFE (KSO-SFE) is cytotoxic towards human cervical cancer cell line [ 7 ]. (hindawi.com)
  • The aim of the present study was to evaluate the influence of angiotensin II (Ang II) on human EC cells. (spandidos-publications.com)
  • By doing so, the study will provide a more complete picture of care received by women in this age Selonsertib in vitro group. (sirna-library.com)
  • 99:209 -215.doi:10.1007/s11060 - 010 - 0129 -5 cell carcinoma-a population-based study in with and without cervical cancer in Tbilisi, Georgia. (who.int)
  • Nevertheless, prior to carrying out the transcriptome evaluation research the relatives toxicity of such rotenone dosages was determined by assaying their results on SK-N-MC cells expansion and loss of life. (scienceexhibitions.org)
  • Antioxidants are compounds that help protect the body against the damaging effects of free radicals, which are unstable molecules that can damage cells and contribute to aging and disease. (chagamushrooms.net)