• 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-test was used to observe the proliferation of human breast cancer MCF-7 and MDA-MB-231 cells induced on VALD-3. (researchsquare.com)
  • To investigate the effects of silibinin on the growth of oral cancer cells, cell proliferation and anchorage-independent colony formation tests were conducted on YD10B and Ca9-22 oral cancer cells. (jcancer.org)
  • Silibinin effectively suppressed YD10B and Ca9-22 cell proliferation and colony formation in a dose-dependent manner. (jcancer.org)
  • Our results further indicate that MCT4 promotes proliferation and survival by altered cell cycle regulation and cell death mechanisms. (hindawi.com)
  • For this purpose, we designed an MCT4 overexpression and knockdown/inhibition model to explore the functional consequences of MCT4 expression on cancer cell metabolism, survival, proliferation, migration, invasion potential, and angiogenesis as well as on cell cycle profiles and cell death mechanisms. (hindawi.com)
  • Our study demonstrates that survivin and APE1/Ref-1 are significantly higher in human prostate cancer specimens compared to noncancerous controls and that APE1/Ref-1 redox-specific inhibition with small molecule inhibitor, APX3330 and a second-generation inhibitor, APX2009, decreases prostate cancer cell proliferation and induces cell cycle arrest. (oncotarget.com)
  • Xanthoxyletin significantly inhibited the proliferation of oral cancer cells (IC₅₀, 10-30 µM) with lower cytotoxicity for normal cells. (medscimonit.com)
  • Detailed knowledge of cancer cell proliferation mechanisms would not only benefit understanding of cancer progression, but may also provide new clues for developing novel therapeutic strategies. (biomedcentral.com)
  • We reported that MNX1 is responsible for sustaining bladder cancer cell proliferation. (biomedcentral.com)
  • Furthermore, MNX1 overexpression accelerated bladder cancer cell proliferation and tumorigenicity both in vitro and in vivo, whereas MNX1 downregulation arrested it. (biomedcentral.com)
  • Generally, dysregulation in cell cycle progression results in the release of proliferative signals in cancer cells, consequently disrupting cell number homeostasis and causing uncontrolled cell proliferation. (biomedcentral.com)
  • We identified kinases within the insulin signaling pathway and centrosome cycle as regulators of ependymoma cell proliferation, and their corresponding inhibitors as potential therapies. (nih.gov)
  • Additionally, GEM or GEM+CDDP at low concentrations dramatically facilitated the proliferation, migration, and invasion of tumor cells in vitro . (researchsquare.com)
  • Effects of taspine on proliferation and apoptosis by regulating caspase-3 expression and the ratio of Bax/Bcl-2 in A431 cells. (rain-tree.com)
  • Proanthocyanidin derived from the leaves of Vaccinium virgatum suppresses platelet-derived growth factor-induced proliferation of the human hepatic stellate cell line LI90. (rain-tree.com)
  • Metabolic reprogramming is an essential mechanism by which cells rewire their activity to promote cell proliferation and growth. (asn-online.org)
  • Finally, arginine depletion in vivo and in vitro reduced the mTOR signaling pathway, cell proliferation, and kidney cyst overload. (asn-online.org)
  • Arginine depletion ameliorates PTC signaling and cell proliferation which are significant contributors to cyst development in TSC. (asn-online.org)
  • Low PDCD4 level was associated with reduced proliferation but not apoptosis or phosphorylation of endothelial nitric oxide synthase caused by pulsatile shear stress to help maintain the homeostasis of endothelial cells. (plos.org)
  • Ample evidence shows that pro-atherosclerotic disturbed flow induces sustained activation of atherogenic genes in endothelial cells to promote their oxidation, inflammation, cell cycle progression and proliferation, whereas pulsatile shear stress tends to maintain endothelial cells in a quiescent and less proliferative state with a low level of oxidation and inflammation [16] , [17] . (plos.org)
  • Although steady-state conditions revealed no increase in primitive cell proliferation in p21-null mice, a significantly larger fraction of quiescent neural precursors was activated in the hippocampus and subventricular zone after brain ischemia. (rupress.org)
  • Therefore, p21 is an intrinsic suppressor to neural regeneration after brain injury and may serve as a common molecular regulator restricting proliferation among stem cell pools from distinct tissue types. (rupress.org)
  • Poisson-event-based analysis of cell proliferation. (swansea.ac.uk)
  • Additionally, CRABP1 knockdown significantly suppressed the proliferation, migration and invasion of gastric cancer cell lines. (nagoya-u.ac.jp)
  • SFK inhibition also induced muscle differentiation in RMS cells by affecting the NOTCH3 receptor-p38 mitogen-activated protein kinase (MAPK) axis, which regulates the balance between proliferation and differentiation. (oncotarget.com)
  • In vitro, we identified the synergistic effect of metformin plus FOLFOX by functional assays including cell proliferation, migration, cell cycle and apoptosis and also analyze the target protein by using Western blot. (ncri.org.uk)
  • However, proliferation of cardiomyocytes induced by nuclear-targeted cyclin D1 (D1NLS) stops after one or two rounds of cell cycles due in part to accumulation of p27Kip1, an inhibitor of cyclin-dependent kinase (CDK). (elsevierpure.com)
  • Thus, expression of S-phase kinase-associated protein 2 (Skp2), a negative regulator of p27Kip1, significantly enhances the effect of D1NLS and CDK4 on cardiomyocyte proliferation in vitro. (elsevierpure.com)
  • In vitro as well as ex vivo, the whole mistletoe extract viscumTT led to strong inhibition of proliferation and synergistic apoptosis induction in osteosarcoma cells. (biomedcentral.com)
  • An image depicting head and neck squamous cell carcinoma in vitro can be seen below. (medscape.com)
  • Head and neck squamous cell carcinoma in vitro (cell culture). (medscape.com)
  • This study is the first to show that VALD-3 induces apoptosis and cell cycle arrest in human breast cancer cells by suppressing Wnt/β-catenin signaling, indicating that it could be a potential drug for the treatment of breast cancer. (researchsquare.com)
  • DNA damage, cell apoptosis and cell cycle arrest were examined by flow cytocytometry. (bvsalud.org)
  • VALD-3 also regulated the Wnt/β-catenin signaling pathway in breast cancer cells, inhibiting the activation of downstream molecules. (researchsquare.com)
  • Antitumor activity of taspine by modulating the EGFR signaling pathway of Erk1/2 and Akt in vitro and in vivo . (rain-tree.com)
  • A novel angiogenesis inhibitor impairs LoVo cell survival via targeting against human VEGFR and its signaling pathway of phosphorylation. (rain-tree.com)
  • The phosphoinositide-3 kinase signaling pathway is implicated in the development of malignancy and promotes cell-cycle progression and resistance to apoptosis. (mesothelioma-line.com)
  • Further, we innovatively demonstrated that notch signaling pathway is responsible for in vitro CD201 + hematopoietic cell induction from mouse PSCs. (biomedcentral.com)
  • The effects of silibinin on the migration and invasion of oral cancer cells were evaluated using transwell assays. (jcancer.org)
  • Distinct hallmarks of cancer were examined using in silico analysis, various in vitro cell culture assays, and ex vivo organotypic rat brain slice culture model. (hindawi.com)
  • We investigated the impact on tumor malignancy in F98 glioma cells using in silico analysis, in vitro cell culture assays, and ex vivo vascular organotypic glioma impact model (VOGIM) [ 35 ] by implanting F98 cells into rat brain slices. (hindawi.com)
  • B.A. Osborne, S.W. Smith, Z.-G. Liu, K.A. McLaughlin, and L.M. Schwartz, Transient Transfection Assays to Examine the Requirement of Putative Cell Death Genes. (nhbs.com)
  • Using a mouse model of ependymoma-a chemoresistant brain tumor-we combined multicell high-throughput screening (HTS), kinome-wide binding assays, and in vivo efficacy studies, to identify potential treatments with predicted toxicity against neural stem cells (NSC). (nih.gov)
  • This study aimed to investigate the effects of xanthoxyletin, a plant-derived coumarin, on human oral squamous cancer cells in vitro and in mouse xenografts in vivo . (medscimonit.com)
  • risk of esophageal squamous cell carcinoma. (who.int)
  • PMID:18786442 mutations and the risk of esophageal squamous cell carcinoma. (who.int)
  • Arbyn M, Bergeron C, Klinkhamer P, Martin-Hirsch squamous cell carcinoma in a high-risk region in Iran. (who.int)
  • In the present study, the TCEE treatment induced cell cycle arrest and suppressed cell growth on both Hep3B and HepJ5 cells. (hindawi.com)
  • Moreover, it induced cell cycle arrest in the G0/G1 phase, apoptosis, and ROS generation in these cells. (jcancer.org)
  • Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. (nature.com)
  • The first is a stochastic model of the progression of cell cycle states, which is applied to experimental data for two synchronized isogenic MMR + and MMR − colon cancer cell lines treated with and without IUdR. (aacrjournals.org)
  • In cancer progression, FOXO3a inhibition stimulates cell transformation and angiogenesis ( 9 ). (spandidos-publications.com)
  • The prevailing model during cell cycle progression is that cyclin-dependent kinase (CDK) activity increases during G1-S transition, which involves the formation of a cyclin-CDK complex. (biomedcentral.com)
  • Therefore, better understanding of how the cell cycle transits would aid comprehension of bladder cancer progression and provide new clues for developing novel therapeutic strategies. (biomedcentral.com)
  • These findings indicate that, the continuous low-dose administration of GEM and GEM+CDDP can promote tumorigenesis and tumor progression in vivo by inhibiting apoptosis, mobilizing BMDCs, and promoting angiogenesis in certain dose ranges. (researchsquare.com)
  • This study observes a previously neglected pharmacological phenomenon and investigates its mechanism of that the continuous low-dose administration of some antineoplastic agents in certain dose ranges can promote tumorigenesis and tumor progression in vitro and in vivo , through stimulation of tumor cell functions directly as well as enhancement of tumor angiogenesis by BMDCs recruitment indirectly. (researchsquare.com)
  • Although PDCD4 in general suppresses the development and progression of tumors, its specific biological functions differ by cell type [8] . (plos.org)
  • In this review, we focused our attentions on carcinogenic metal-induced signal transduction pathways leading to the activation of NF-kappaB, cell apoptosis and cell cycle progression, three crucial steps or events involved in the transformation and carcinogenesis. (cdc.gov)
  • Decades of efforts have attempted to differentiate the pluripotent stem cells (PSCs) into truly functional hematopoietic stem cells (HSCs), yet the problems of low differentiation efficiency in vitro and poor hematopoiesis reconstitution in vivo still exist, mainly attributing to the lack of solid, reproduced, or pursued differentiation system. (biomedcentral.com)
  • Although hematopoietic stem and progenitor cells (HSPCs) enable autologous treatment of blood disorders, de novo generation of hematopoietic cells from pluripotent stem cells still compromises the unlimited high yield and rich hematopoiesis engraftment potential [ 2 ]. (biomedcentral.com)
  • Sozzi E, Nilsson F, Kajtez J, Parmar M, Fiorenzano A. Generation of Human Ventral Midbrain Organoids Derived from Pluripotent Stem Cells . (lu.se)
  • relative mRNA expression was evaluated by real-time polymerase chain reaction (RT-PCR), and relative protein expressions were measured by western blot assay in an in vitro study. (medscimonit.com)
  • Using hybrid/integrative modeling, involving multiple experimental and computational methods, we have been able provide mechanistic insights into the role of cryptochromes a core clock protein, in regulating circadian period length, thus directly relating protein structure and dynamics to in vitro and in vivo experimental observations [1]. (imsc.res.in)
  • Due to the role of oncogenic transcriptional activators NFĸB and STAT3 in survivin protein expression, and APE1/Ref-1 redox activity regulating their transcriptional activity, we assessed selective inhibition of APE1/Ref-1's redox function as a novel method to halt prostate cancer cell growth and survival. (oncotarget.com)
  • L.T. Bemis, F.J. Geske, and R. Strange, Use of the Yeast Two-Hybrid System for Identifying the Cascade of Protein Interactions Resulting in Apoptotic Cell Death. (nhbs.com)
  • FoxO3a silencing resulted in decreased protein expression of FoxO3a, increased in vitro carcinogenesis and CSC characteristics, with no appreciable effects on AMPK and ACC phosphorylation, and displayed similar activities to those neutralizing the effects of casticin on in vitro carcinogenesis and CSC characteristics. (spandidos-publications.com)
  • Application of unidirectional pulsatile shear stress to human umbilical vein endothelial cells (HUVECs) decreased PDCD4 protein but not mRNA level. (plos.org)
  • For this purpose, the function of cellular retinoic acid binding protein 1 (CRABP1) in gastric cancer cells was investigated and it was determined whether it serves as a novel biomarker for gastric cancer. (nagoya-u.ac.jp)
  • The main result reported by the authors was as follows: "The use of phosphomutant Ascl1 in place of the wild-type protein significantly promotes neuronal maturity after human fibroblast reprogramming in vitro. (genengnews.com)
  • Dr. Philpott and her collaborators are aware that the protein control mechanisms that promote neuron maturation are similar to those involved in the maturation of important cells in other tissues such as pancreatic islets, the cell type that fails to function effectively in type 2 diabetes. (genengnews.com)
  • The mechanisms by which specific ATP2A2 mutations impact the function of the ATP2A2 protein have been investigated using an in vitro model. (medscape.com)
  • The investigators found that the resultant transfected cells showed defects in ATP2A2 protein expression (15 mutants), ATP hydrolysis (29 mutants), calcium transport (4 mutants), and calcium binding and kinetics (3 mutants). (medscape.com)
  • In a different study, in which researchers systematically analyzed mutations identical to those found in patients with Darier disease, mutant SERCA2 protein aggregates were found to cause stress to the endoplasmic reticulum, subsequently inducing cell apoptosis. (medscape.com)
  • Here we describe a different method of transgene regulation by the use of the human glial ®brillary acidic protein (GFAP) promoter. (lu.se)
  • In animals receiving excitotoxic lesions of the striatum we detected an eightfold increase of green ¯uorescent protein (GFP)-expressing cells. (lu.se)
  • Materials and Methods Reagents DO1 antibody was a kind gift from Dr Borivoj Vojtesek. (icem2012.org)
  • Materials and Methods: Twenty-four nude mice were subcutaneously injected with HeLa cells. (iiarjournals.org)
  • MATERIALS AND METHODS: This study was a systematic review and meta-analysis of randomized controlled trials (RCTs). (bvsalud.org)
  • MATERIALS AND METHODS: DOP was extracted, purified and structurally characterized. (bvsalud.org)
  • Materials and methods approach to regulate transgenic expression. (lu.se)
  • The cyclin-dependent kinase inhibitor, p21 cip1/waf1 (p21), maintains hematopoietic stem cell quiescence, and we evaluated its role in the regenerative response of neural tissue after ischemic injury using the mice deficient in p21. (rupress.org)
  • Overall, our findings suggest that SFK inhibition, besides reducing RMS cell growth and invasive potential, could also represent a differentiation therapeutic strategy for RMS. (oncotarget.com)
  • The method, developed by researchers at the University of Cambridge, was described online May 12 in Development, in an article entitled "The phosphorylation status of Ascl1 is a key determinant of neuronal differentiation and maturation in vivo and in vitro. (genengnews.com)
  • By manipulating the signals that transcription factors send to the cells, the researchers, led by Anna Philpott, Ph.D., were able to promote cell differentiation and maturation, even in the presence of conflicting signals that were directing the cell to continue dividing. (genengnews.com)
  • This multisite phosphorylation is a crucial regulator of the ability of Ascl1 to drive neuronal differentiation and maturation in vivo in the developing embryo. (genengnews.com)
  • To arrive at such results, suggested Dr. Philpott, "not only do you have to think about how you start the process of cell differentiation in stem cells, but you also have to think about what you need to do to make differentiation complete-we can learn a lot from how cells in developing embryos manage this. (genengnews.com)
  • In this study, we established an in vitro differentiation system yielding in vivo hematopoietic reconstitution hematopoietic cells from mouse PSCs through a 3D induction system followed by coculture with OP9 stromal cells. (biomedcentral.com)
  • We interestingly found that our 3D+OP9 system enriched a higher percentage of CD201 + c-kit + cells that showed more similar HSC-like features such as transcriptome level and CFU formation ability than CD201 - c-kit + cells, which have not been reported in the field of mouse PSCs hematopoietic differentiation. (biomedcentral.com)
  • Altogether, our findings lay a foundation for improving the efficiency of hematopoietic differentiation and generating in vivo functional HSC-like cells from mouse PSCs for clinical application. (biomedcentral.com)
  • Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
  • The expression of pro-apoptotic proteins and anti-apoptotic proteins in MCF-7 and MDA-MB-231 cells was investigated by Western blotting. (researchsquare.com)
  • Furthermore, silibinin inhibited the migration and invasion abilities of YD10B and Ca9-22 cells by regulating the expression of proteins involved in the epithelial-mesenchymal transition. (jcancer.org)
  • In vitro transcription-translation Proteins were synthesised by in vitro transcription/translation using the PURESYSTEM kit (NEB). (icem2012.org)
  • Cell-cycle arrest, activation of associated apoptotic proteins, and inhibition of apoptosis were also observed in tumor cells. (researchsquare.com)
  • Thus, the effects of EP on modulating PDCoV-induced apoptosis and the impact of PDCoV on TJ proteins need to be elucidated in vivo. (biomedcentral.com)
  • Additionally, down-regulation of inhibitor of apoptosis proteins (IAPs), such as BIRC5 and XIAP was observed in Ewing's sarcoma cell lines (Twardziok et al. (biomedcentral.com)
  • To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
  • These vectors have a number of appealing features including the expression by using the machinery of the host cell instead of depending abilities to ef®ciently transduce cells in the central nervous system, on recombinant regulatory proteins. (lu.se)
  • These findings indicated that CRABP1 contributes to the malignant phenotype of gastric cancer cells and may serve as a biomarker for prognosing recurrence after curative resection, particularly peritoneal dissemination. (nagoya-u.ac.jp)
  • This highly malignant tumor has traditionally been considered to arise from committed skeletal muscle precursor cells that fail to differentiate [ 1 ], although some RMSs develop in organs lacking a skeletal muscle component [ 2 , 3 ]. (oncotarget.com)
  • Malignancies involving mesothelial cells that normally line the body cavities, including the pleura (see the image below), peritoneum, pericardium, and testis, are known as malignant mesothelioma. (medscape.com)
  • In contrast to results with 2D culture, observed mitotic arrest was relatively low, suggesting involvement of additional mechanisms in their antitumor effect in vivo . (nature.com)
  • The ethanolic extract of T. camphoratus (TCEE) which contains abundant bioactive compounds including triterpenoids and polysaccharides also has antitumor effects in various human cancer cell lines. (hindawi.com)
  • The aims of this study are to clarify the antitumor effects of TCEE on human hepatocellular carcinoma cells and also evaluate the combination drug effects with conventional chemotherapy agents, cisplatin and doxorubicin. (hindawi.com)
  • This TCEE also demonstrates antitumor properties such as the induction of cell cycle arrest and activation of apoptosis on human colon, lung, melanoma, osteosarcoma, and pancreatic cancer cells [ 16 - 19 ]. (hindawi.com)
  • In contrast, the antitumor effects and related biological mechanism of TCEE as well as the combination drug effects with conventional chemotherapy agents remain unclear particularly in human hepatocellular carcinoma cells. (hindawi.com)
  • The aims of this preclinical study are to evaluate the capability of TCEE to suppress human hepatocellular carcinoma cells and clarify the related antitumor effects. (hindawi.com)
  • The antitumor activity and survival analysis of VALD-3 in vivo was determined by the nude mice xenograft assay. (researchsquare.com)
  • Background/Aim: Demethoxycurcumin (DMC), a derivate of curcumin from natural plants, exerts antitumor effects on various human cancer cells in vitro and in vivo. (iiarjournals.org)
  • Therefore we investigated the antitumor effects of DMC on a HeLa cell xenograft model in nude mice in this study. (iiarjournals.org)
  • Because APE1/Ref-1 is expressed and elevated in prostate cancer, we sought to characterize APE1/Ref-1 expression and activity in human prostate cancer cell lines and determine the effect of selective reduction-oxidation (redox) function inhibition on prostate cancer cells in vitro and in vivo . (oncotarget.com)
  • Croton lechleri sap and isolated alkaloid taspine exhibit inhibition against human melanoma SK23 and colon cancer HT29 cell lines. (rain-tree.com)
  • Inhibition of epidermal growth factor receptor resulted in apoptotic cell death and Akt hypophosphorylation in mesothelioma cell lines. (mesothelioma-line.com)
  • Insulin-like growth factor receptor inhibition led to apoptotic cell death without affecting Akt phosphorylation. (mesothelioma-line.com)
  • We have demonstrated that epidermal growth factor receptor inhibition leads to apoptotic cell death through downregulation of phosphoinositide-3 kinase signaling in mesothelioma cell lines, whereas insulin-like growth factor receptor inhibition leads to apoptosis independent of phosphoinositide-3 kinase. (mesothelioma-line.com)
  • Results: DMC significantly reduced tumor weights and volumes of HeLa cell xenografts in mice, indicating the suppression of growth of xenograft tumors. (iiarjournals.org)
  • The aim of this study was to examine the effects and mechanisms of tenacigenin B in lymphoma treatment by in vitro and in vivo experiment. (medscimonit.com)
  • In this study, we investigated the cytotoxic and apoptotic effects of VALD-3, a Schiff base ligand synthesized from o-vanillin derivatives, on human breast cancer cells and the possible underlying mechanisms. (researchsquare.com)
  • However, the exact mechanisms leading to tubular cell damage and cyst formation remain poorly understood. (asn-online.org)
  • Although the signaling mechanisms governing myelination are not fully understood, NF-κB activation in Schwann cells has been implicated as a key regulator in vitro . (jneurosci.org)
  • Neural precursor cells from adults have exceptional proliferative and differentiative capability in vitro yet respond minimally to in vivo brain injury due to constraining mechanisms that are poorly defined. (rupress.org)
  • The aim of this study is to evaluate the potential of EP as a treatment for PDCoV in vivo and elucidate the possible mechanisms. (biomedcentral.com)
  • [ 6 ] These events lead the cancer cell to escape normal cell growth and control mechanisms, to avoid system control mechanisms (ie, immunologic surveillance), and to establish a nutrient supply. (medscape.com)
  • CellCognition: time-resolved phenotype annotation in high-throughput live cell imaging. (nature.com)
  • By xenografting human breast cancer cells into nude mice, we found that VALD-3 significantly suppressed tumor cell growth while showing low toxicity against major organs. (researchsquare.com)
  • In Vivo, seven weeks-old male BALB/c nude mice were randomly divided into four groups. (ncri.org.uk)
  • To better understand Plasmodium biology, researchers can whether proceed to in vitro studies or use in vivo models. (wjgnet.com)
  • In parallel, the development of animal models opened new opportunities to study parasite biology in vivo . (wjgnet.com)
  • To the best of our knowledge, this is the first study that used predicted miRNAs to determine the features that can separate the diseased samples (T2D or AD) from the normal ones, instead of using conventional non-biology-based feature selection methods. (frontiersin.org)
  • Reverse transcription-quantitative (RT-q) PCR and a PCR-array method were used to determine whether the expression of CRABP1 mRNA in gastric cancer cell lines correlated with the expression of cancer-related genes. (nagoya-u.ac.jp)
  • Methods: Transcriptome analysis of surgically resected metastatic tissue from GC patients and noncancerous tissue was performed to identify novel metastasis-related genes. (nagoya-u.ac.jp)
  • 2003). The therapeutic effects of different genes delivered by ment of the hGFAP promoter used here has been extensively studied and lentiviral vectors have been documented in a number of animal found to be up-regulated after several different stimuli both in vitro and models, both in rodents and primates, and examples include delivery in vivo in transgenic mice (Brenner et al. (lu.se)
  • We further demonstrate that Nutlin-3 inhibits the TCTP-HDM2 connection both in vitro and ex vivo, therefore highlighting an additional mechanism through which Nutlin-3 abrogates HDM2 function. (icem2012.org)
  • However, whether casticin inhibits in vitro carcinogenesis and cancer stem cell (CSC) characteristics, and whether casticin activates FoxO3a in small cell lung cancer (SCLC) cells remain unclear. (spandidos-publications.com)
  • However, whether casticin inhibits in vitro carcinogenesis and CSC characteristics in the SCLC H446 cell line, and activates FoxO3a remains unclear. (spandidos-publications.com)
  • A novel taspine analog, HMQ1611, inhibits growth of non-small cell lung cancer by inhibiting angiogenesis. (rain-tree.com)
  • Tas13D inhibits growth of SMMC-7721 cell via suppression VEGF and EGF expression. (rain-tree.com)
  • FOXC1 silencing inhibits the epithelial‑to‑mesenchymal transition of glioma cells: Involvement of β‑catenin signaling. (cancerindex.org)
  • Programmed cell death 4 (PDCD4) is an important tumor suppressor in the development of various human cancers [1] and inhibits translation rather than transcription. (plos.org)
  • Analysis of mitosis and antimitotic drug responses in tumors by in vivo microscopy and single-cell pharmacodynamics. (nature.com)
  • This process enables tumor cells, including those within the core of the heterogeneous tumor mass to survive, despite being at a distance from blood vessels that supply their metabolic needs [ 16 , 17 ]. (hindawi.com)
  • Emerging evidence suggests that cancer stem cells (CSCs), a subpopulation of tumor cells, have the properties of self-renewal, heterogeneous progeny, drug-resistance, and carcinogenesis in vitro and in vivo ( 4 , 5 ). (spandidos-publications.com)
  • SI221, which showed to be mainly effective against the SFK member YES, significantly reduced cell viability and induced apoptosis, without affecting non-tumor cells, such as primary human skin fibroblasts and differentiated C2C12 cells. (oncotarget.com)
  • The MTT assay assessed cell viability, and autophagy was detected by electron microscopy. (medscimonit.com)
  • In vitro function of MNX1 (Motor neuron and pancreas homeobox 1) in bladder cancer cell was evaluated using MTT assay, colony formation assay, and bromodeoxyuridine incorporation assay. (biomedcentral.com)
  • After exposure of mesothelioma lines to kinase inhibitors, a cell viability assay was performed, cell-cycle analysis was performed to determine the percentage of apoptosis, and Western blot analysis was performed for phosphorylated Akt. (mesothelioma-line.com)
  • The in vivo hematopoietic reconstitution potential of c-kit + cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. (biomedcentral.com)
  • RESULTS: The results showed that DOP achieve photoaging protection by inhibiting oxidative stress, alleviating cell cycle arrest and apoptosis, and enhancing autophagy flux in photoaged HaCaT cells. (bvsalud.org)
  • ViscumTT treatment results in synergistic apoptosis induction in osteosarcoma cells in vitro and ex vivo. (biomedcentral.com)
  • The c-kit + cells from 3D self-assembling peptide induction system followed by the OP9 coculture system possessed apparently superiority in terms of in vivo repopulating activity than that of 3D induction system followed by the 0.1% gelatin culture. (biomedcentral.com)
  • Expression of cell cycle inhibitors, P21 and P27, and activation of apoptosis executer enzyme, caspase-3, were also induced by TCEE. (hindawi.com)
  • We assessed whether cell cycle inhibitors that restrict stem cell populations in other tissues may participate in limiting neural stem cell reactivity in vivo. (rupress.org)
  • The physiology and behavior of almost all living organisms on earth is synchronized to a 24-hour solar cycle by a well-regulated molecular clock mechanism. (imsc.res.in)
  • Because of breakthroughs such as the "switching" mechanism of the p53 tumor suppressor gene, as well as many technical innovations, the number of investigators of cell death has rapidly expanded over the last few years. (nhbs.com)
  • These findings reveal a novel mechanism for regulating AMPK/FoxO3a signaling in response to casticin, suggesting a new strategy for SCLC therapy by targeting cancer stem‑like cells. (spandidos-publications.com)
  • However, there is no comprehensive study on the effect of DOP on skin photoaging combined with in vitro and in vivo models, and its specific mechanism is still unclear. (bvsalud.org)
  • Apoptosis is a double-edged sword mechanism, which is used by the host to eliminate viruses and is manipulated by the virus to cause cytopathic effects in vitro and/or tissue damage in vivo [ 10 ]. (biomedcentral.com)
  • Several research findings demonstrate the role of miRNAs in β-cell stimulus-secretion coupling and insulin biosynthesis. (frontiersin.org)
  • Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)
  • Stable F98 glioma cell lines with MCT4 overexpression, normal expression, and knockdown were generated. (hindawi.com)
  • In summary, our data highlight MCT4 /SLC16A3 as a key gene for distinct hallmarks of tumor malignancy in glioma cells. (hindawi.com)
  • We determine a comprehensive map of lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs), combining RNA-Seq and CAGE-Seq. (nature.com)
  • Thereafter, a distinct subpopulation of endothelial cells lining the cardinal vein starts differentiating by expressing the TF PROX1, the master regulator of lymphatic endothelial cell (LEC) identity, via the TFs SOX18 and COUPTFII. (nature.com)
  • Loss of corneal endothelial cells (CECs) bears disastrous consequences for the patient, including corneal clouding and blindness. (lww.com)
  • 1 If the number of corneal endothelial cells (CECs) falls below a certain threshold because of traumatic injury, disease, or normal aging processes, functionality of the CE is decreased, and the cornea swells and becomes milky, leading to eventual vision loss. (lww.com)
  • We investigated whether atheroprotective unidirectional pulsatile shear stress affects the expression of PDCD4 in endothelial cells. (plos.org)
  • En face co-immunostaining of the mouse aortic arch revealed a low level of PDCD4 in endothelial cells undergoing pulsatile shear stress. (plos.org)
  • However, the action of PDCD4 in endothelial cells is unclear. (plos.org)
  • Vascular endothelial cells, as a monolayer in direct contact with the flowing blood, bear the most of the wall shear stresses and have important homeostatic functions in response to stress [16] . (plos.org)
  • [ 22 ] Investigators transfected a fibroblast cell line with 51 different mutations seen in keratosis follicularis (Darier disease) pedigrees. (medscape.com)
  • Tenacigenin B had anti-tumor effects on lymphoma via regulation of Aurora-A in vitro and in vivo . (medscimonit.com)
  • The techniques described facilitate both understanding and regulation of cell death, promising to have immense value in the clinical treatment of cancer. (nhbs.com)
  • During development, the blood vascular system arises from endothelial cell progenitors that differentiate from mesodermal cells, mostly through the expression of the transcription factor (TF) ETV2. (nature.com)
  • Activation of the VEGFA/VEGFR2 signaling and expression of blood vascular endothelial cell (BEC) markers, such as NRP1 and EphrinB2, further differentiate these precursor cells into BECs, which then form the hierarchical network of blood vessels 4 . (nature.com)
  • This internal biological clock regulates a host of cellular responses to the environment, ranging from gene expression and cell division in cyanobacteria, to photosynthesis in plants and finally to the sleep/wake cycles in mammals (commonly referred as circadian rhythms). (imsc.res.in)
  • These trends were associated with an increase in urea cycle metabolites and the rate-limiting enzyme, argininosuccinate synthase 1 (ASS1), expression levels. (asn-online.org)
  • Rapamycin treatment reversed the increase in ASS1 expression in Tsc1 Knockout HK2 cell, emphasizing the contribution of the Tsc1-mTORC1 pathway to ASS1 expression. (asn-online.org)
  • Western blot analysis was performed to determine the relative expression of epidermal growth factor receptor, insulin-like growth factor receptor, and platelet-derived growth factor receptor in the mesothelioma cell lines I-45 and REN and the mesothelial line Met5a. (mesothelioma-line.com)
  • One and 28 d after acute exposure, and 1, 28, and 90 d following sub-chronic exposure, bronchoalveolar lavage was performed on the left lung to collect cells and fluid for analyses, the apical right lobe was preserved for histopathology, and the right cardiac and diaphragmatic lobes were processed for gene expression analyses. (cdc.gov)
  • immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
  • B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)
  • There are a number of hypothesized that regulating the transgene with a GFAP promoter in a different vectors that transduce cells in the brain in a slightly different viral vector would give rise to a high transgenic expression in the manner (for a review see, e.g. (lu.se)
  • 2000). We of glial cell line-derived neurotrophic factor (GDNF) in models of have recently reported that this vector directs transgene expression to Parkinson's disease (Georgievska et al. (lu.se)
  • In vitro, we confirmed that metformin plus FOLFOX group could induce apoptosis significantly compared with FOLFOX only or metformin only group. (ncri.org.uk)
  • In addition to the new in vitro systems, in recent years there were also significant advances in the development of new animal models that allows studying the entire cell cycle of human malaria. (wjgnet.com)
  • In this paper, we review the different protocols available to study human Plasmodium species either by using stem cell or alternative animal models. (wjgnet.com)
  • Nevertheless, no reports have disclosed whether DMC can affect the growth of human cervical cancer cells in vivo. (iiarjournals.org)
  • The experimental design for the demethoxycurcumin (DMC) treatments in human cervical HeLa cell-bearing mice. (iiarjournals.org)
  • The study included SCC-1 human oral cancer cells and EBTr normal embryonic bovine tracheal epithelial cells, which were treated with 0 µM, 5 µM, 10 µM, and 20 µM of xanthoxyletin for 24 hours. (medscimonit.com)
  • Because human mesenchymal stem cells are known to be sensitive to their mechanical environments, we investigated the mechanotransductive potential of Descemet membrane-like microtopography (DLT) to differentiate human mesenchymal stem cells into CEC-like cells. (lww.com)
  • 2 Despite having high metabolic activity, human CECs (hCECs) do not proliferate in vivo because these cells arrest in the G1 phase of the cell cycle. (lww.com)
  • Sangre de grado ( Croton palanostigma ) induces apoptosis in human gastrointestinal cancer cells. (rain-tree.com)
  • This technologic and biologic revolution continued through the 1960s to today, and the explosion in technology has fueled the current expansion of knowledge into the working of the human cell. (medscape.com)
  • As these kinds of health effects data become available and methods to assess levels of significant human exposure improve, these MRLs will be revised. (cdc.gov)
  • Artificial reproduction of Descemet membrane with respect to topography and similar stiffness offers a potential innovative way to bioengineer a functional CEC monolayer from autologous stem cells. (lww.com)
  • For example, the methods used to generate nerve cells from patient fibroblasts tend to yield low numbers of cells, and those that are produced are not fully functional. (genengnews.com)
  • In fact, only using the OP9 co-culture system combined with hematopoietic related cytokines, we hardly obtained the in vivo functional reconstitution hematopoietic cells from PSCs [ 9 ]. (biomedcentral.com)
  • Intracellular signal transduction of cells in response to carcinogenic metals. (cdc.gov)
  • This review summarizes current knowledge and our recent studies concerning intracellular signal transduction pathways initiated by carcinogenic metals and the cross-talk that occurs among these pathways in cells in response to metals. (cdc.gov)
  • Six parasites showed in vitro resistance to sodium stibogluconate using murine J774 macrophage amastigote testing method. (who.int)
  • Quantification of cell-cycle state at a single-cell level is essential to understand fundamental three-dimensional (3D) biological processes such as tissue development and cancer. (nature.com)
  • Together, our study provides several lines of evidence supporting the intriguing concept that every cell type expresses precise lncRNA signatures to control lineage-specific regulatory programs. (nature.com)
  • Thanks to recent progresses, stem cells have been extensively employed to study Plasmodium liver and blood cycle in vitro . (wjgnet.com)
  • The present study uses the integration of probabilistic mathematical models and experimental data on MMR − versus MMR + cells to describe the effects of IUdR incorporation upon the cell cycle for the purpose of increasing IUdR-mediated radiosensitivity in MMR − cells. (aacrjournals.org)
  • Cell Death provides a current and comprehensive collection of methods for the study of cell death. (nhbs.com)
  • In the tradition of this long-standing series, this volume's user-friendly recipes and more than 100 tables and figures make the study of cell death accessible across life science disciplines. (nhbs.com)
  • A.J. McGahon, S.J. Martin, R.P. Bissonnette, A. Mahboubi, Y. Shi, R.J. Mogil, W.K. Nishioka, and D.R. Green, The End of the (Cell) Line: Methods for the Study of Apoptosis In Vitro. (nhbs.com)
  • Our in vitro study revealed that ergosterol peroxide (EP) from the mushroom Cryptoporus volvatus has efficient anti-PDCoV properties. (biomedcentral.com)
  • This prospective study was conducted with 96 spent CM from patients undergoing IVF cycle, in order to determine relationships of Cell-free DNA levels in embryo CM with embryo cleavage quality on day 3. (who.int)
  • Fruits, vegetables and endometrial cancer risk in women in the European forces in synthesizing evidence on new methods for the risk of cancer: a multisite case-control study in Prospective Investigation into Cancer and Nutrition cervical cancer prevention. (who.int)
  • In the metabolic symbiosis model, these cells have been proposed to overexpress MCT4 to release high amounts of lactate produced during anaerobic glycolysis into the tumor microenvironment. (hindawi.com)
  • Metabolome analysis of whole kidneys and proximal tubular cells (PTCs) from TSC mice showed major perturbation in several metabolic pathways, mainly the arginine biosynthesis pathway. (asn-online.org)
  • Two cell lineages are observed on the embryonic-abembryonic axis of the blastocyst: the inner cell mass and the trophectoderm. (bioone.org)
  • In mouse embryos, cells seem prepatterned to become certain cell lineage because the first cleavage plane has been related with further embryonic-abembryonic axis at the blastocyst stage. (bioone.org)
  • Blastocysts were then classified according to the allocation of the labeled cells in the embryonic and/or abembryonic part of the blastocyst. (bioone.org)
  • We applied our workflow to quantify cell-cycle effects of three antimitotic cancer drugs over 8 d in HT-1080 fibrosarcoma xenografts in living mice using a data set of 38,000 cells and compared the induced phenotypes. (nature.com)
  • In vivo tumor growth was conducted in BALB/c-nu mice. (biomedcentral.com)
  • CNT in biochemical models and in cells yielding the products engineered CNT represent a possible health risk due to their that did not cause pulmonary inflammation in mice [14]. (cdc.gov)
  • FOXO3a is considered an evolutionarily conserved transcription factor involved in various cellular processes, including cell cycle arrest, DNA repair and tumor suppression ( 8 ). (spandidos-publications.com)
  • Specifically, the researchers accomplished the cell-cycle-dependent phosphorylation of a key reprogramming transcription factor, Ascl1, on multiple serine-proline sites. (genengnews.com)
  • Our results provide direct evidence for the participation of MPO - one of the key-orchestrators of inflammatory response - in the in vivo pulmonary oxidative biodegradation of SWCNT and suggest new ways to control the biopersistence of nanomaterials through genetic or pharmacological manipulations. (cdc.gov)
  • Master molds with inverted DLT were produced by 2-photon lithography (2-PL). To measure the mechanotransductive potential of DLT, mesenchymal stem cells were cultivated on silicone or collagen imprints with DLT. (lww.com)
  • Exposure of mesothelioma cell lines to LY294002, a phosphoinositide-3 kinase inhibitor, results in apoptotic cell death and decreased phosphorylated Akt in vitro and tumor burden reduction in vivo. (mesothelioma-line.com)
  • To achieve this, cells of in vitro produced bovine embryos were traced from the 2-cell stage to the blastocyst stage. (bioone.org)
  • We aimed to examine the molecular processes underlying the effects of silibinin in oral cancer in vitro and in vivo as well as its potential anticancer effects. (jcancer.org)
  • Methods and results: Wistar rats underwent ischaemia/reperfusion injury by ligation of the coronary artery followed by injection of adenovirus vectors for D1NLS and CDK4 with or without Skp2. (elsevierpure.com)
  • RESULTS: No exposure-related changes were identified in histopathology, cytotoxicity, or lavage cell profiles. (cdc.gov)
  • The results revealed a significant increase in Cell-free DNA levels on day 2 CM corresponding to 4 to 6 cell embryos compared to those corresponding to 7 to 8 cel embryos (p=0.04). (who.int)
  • As for day 3 CM, the results showed no significant difference between the Cell-Free DNA levels in CM of 7-8 and those of 4-6 cell embryos (p=0.4). (who.int)
  • More recently, TCTP offers been shown to bind directly to p53, with TCTP overexpression increasing p53 degradation and advertising lung malignancy cell survival [17]. (icem2012.org)
  • Within the tomocyst, a series of mitotic cell divisions take place and, depending on temperature, up to 1000 resulting daughter cells (tomites) are produced. (wikipedia.org)
  • Condeelis, J. & Weissleder, R. In vivo imaging in cancer. (nature.com)
  • Next, we investigated the molecular processes underlying both in vitro and in vivo outcomes of silibinin treatment on oral cancer. (jcancer.org)
  • Western blotting revealed that silibinin downregulated SOD1 and SOD2 and triggered the JNK/c-Jun pathway in oral cancer cells. (jcancer.org)
  • Silibinin considerably reduced the development of oral cancer cells by inducing apoptosis, G 0 /G 1 arrest, ROS generation, and activation of the JNK/c-Jun pathway. (jcancer.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Previous studies in our laboratory have described increased and preferential radiosensitization of mismatch repair-deficient (MMR − ) HCT116 colon cancer cells with 5-iododeoxyuridine (IUdR). (aacrjournals.org)
  • Previous studies by our laboratory have identified a series of 9-amino acridine compounds that block the catalytic cycle of topoisomerase II resulting in apoptosis and cell death in a variety of cancer cell lines. (umn.edu)
  • Casticin, a natural polymethoxyflavone isolated from A. annua, V. trifolia, and V. agnus‑castus induces apoptosis in cancer cells by activating FoxO3a. (spandidos-publications.com)
  • We here demonstrated that casticin decreased sphere‑ and colony‑formation capabilities, and downregulated uPAR and CD133 in second‑generation spheres, which were considered as lung cancer stem‑like cells (LCSLCs), from SCLC H446 cells, in a concentration‑dependent manner. (spandidos-publications.com)
  • Small cell lung cancer (SCLC), a poorly differentiated and highly aggressive tumor, constitutes approximately 15% of all lung cancers ( 1 ). (spandidos-publications.com)
  • Conversely, FOXO3a overexpression suppresses cancer cell growth, induces apoptosis, and reduces tumor size by regulating downstream effectors ( 10 ). (spandidos-publications.com)
  • In addition, MNX1 transcriptionally upregulated CCNE1 and CCNE2 by directly bounding to their promoters, which promoted G1-S transition in the bladder cancer cells. (biomedcentral.com)
  • presented that disulfiram (DSF) was highly toxic to gastric cancer cells in a copper-dependent manner. (biomedcentral.com)
  • CRABP1 mRNA levels varied among gastric cancer cell lines and showed significant positive correlations with numerous epithelial-mesenchymal transition factors. (nagoya-u.ac.jp)
  • Analyses of in vitro cell function, apoptosis, the cell cycle and cancer stemness were performed using GC cell lines with a stable knockout of a candidate gene. (nagoya-u.ac.jp)
  • In turn, as a result of research into the abnormal cancer cell, the basic understanding of the cell has greatly improved. (medscape.com)
  • Cancer is characterized by uncontrolled growth and division of a cell, with extension beyond the normally limiting basement membrane and through the boundaries of normal cells. (medscape.com)
  • This is the multihit theory of tumorigenesis, in which a series of multiple triggering events in the genetic and cellular makeup of a cell ultimately cause cancer. (medscape.com)
  • Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)
  • Identification of a novel topoisomerase inhibitor effective in cells overexpressing drug efflux transporters. (rain-tree.com)