Cell cycle g2 arrest mice cell cycle. Medical search. Frequent questions

Next, flow cytometry analysis showed TiO 2 NPs promoted apoptosis and cell cycle G2/M phase arrest of mouse primary corneal endothelial cells. (aging-us.com)

Immunohistochemical analysis revealed that tumor cell proliferation and apoptosis were higher in irradiated groups compared to the control group. (bioone.org)
Xanthoxyletin significantly inhibited the proliferation of oral cancer cells (IC₅₀, 10-30 µM) with lower cytotoxicity for normal cells. (medscimonit.com)
Although TGF-1 alone did not trigger pericyte proliferation or of genes encoding its receptors in mice leads to vascular defects and embryonic lethality.17C19 TGF-1 is thus a cytokine with a profound effect on microvascular development and angiogenesis. (holyexperiment.org)
We showed inhibition of cell proliferation that was time- and dose-dependent in 5RP7 cells for 24 and 48 h. (tubitak.gov.tr)
Exposure to benzo(a)pyrene affected the expression of genes that are involved in cell cycle regulation in both genotypes, indicating that the presence of unrepaired DNA damage in testis blocks cell proliferation to protect DNA integrity in both DNA repair proficient and deficient animals. (maastrichtuniversity.nl)
Firstly, we found TiO 2 NPs inhibited proliferation and damaged morphology and mitochondria of mouse primary corneal endothelial cells. (aging-us.com)
An aberrant miRNA expression could contribute to cancer development and progression [ 6 , 7 ] and could affect their target genes that are involved in many biological processes, such as cell differentiation, proliferation, apoptosis, metabolism, and development [ 8 ]. (hindawi.com)
Artemisinin dimer oxime - dimer molecule synthesized from artemisinin possesses high bioavailability and marked in vitro anticancer activities against solid tumor-derived cell lines, endothelial cell proliferation, migration, and angiogenic processes. (wjtcm.net)
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-test was used to observe the proliferation of human breast cancer MCF-7 and MDA-MB-231 cells induced on VALD-3. (researchsquare.com)
The cell cycle is the process of accurate self-reproduction and proliferation of a cell. (intechopen.com)
Misregulation of the cell cycle may result in malignant cell proliferation, tumorigenesis or cell death. (intechopen.com)
A vast amount of research exists on the possible molecular mechanisms through which vitamin D affects cancer cell proliferation, cancer progression, angiogenesis, and inflammation. (mdpi.com)
This compound inhibited cell proliferation and Erk signaling activation, induced cell cycle arrest in the S phase, confirming its cytostatic effect, and suppressed tumorigenesis. (moestuininfo.com)
Knockdown of TET1 or ectopic expression of TET2 in T-ALL was associated with genome-wide changes in 5mC and 5hmC enrichment and decreased cell proliferation, suggesting a tumor promoting function of TET1, and a tumor suppressing role for TET2. (biomedcentral.com)
mice represent a useful model for exploring the activation of adult hepatic progenitor cells, since PR-SET7 deficiency leads to cell cycle arrest (Beck knockout mice and investigated the effect of PR-SET7 deficiency in liver organogenesis, hepatocyte proliferation and liver regeneration. (antiviralbiologic.com)
Doxorubicin (DOX) is known as an anticancer drug that acts in the G2/M phase of the cell cycle to arrest cell proliferation and induce cellular senescence (see the upper left figure). (dojindo.com)
It was discovered when pulmonary fibroblasts slowed down their proliferation and eventually ended in cell death after cell passaging had been performed for more than 8 months. (dojindo.com)
During cell growth and proliferation, ubiquitin plays an outsized role in promoting progression through the cell cycle. (intechopen.com)
Notably, the G1/S boundary represents a major barrier to cell proliferation and is universally dysfunctional in cancer cells, allowing for the unbridled proliferation observed in malignancy. (intechopen.com)
Numerous E3 ubiquitin ligases, which facilitate the ubiquitination of specific substrates, have been shown to control G1/S. In this chapter, we will discuss components in the ubiquitin proteasome system that are implicated in G1/S control, how these enzymes are interconnected, gaps in our current knowledge, and the potential role of these pathways in the cancer cycle and disease proliferation. (intechopen.com)

Plk1 protein and activity are low in G1, accumulate during S and G2/M phases, and are rapidly reduced in the late stages of mitosis [ 7 ]. (nature.com)
Flow cytometric analysis evidenced that piperine arrested the cell cycle at growth 2/mitosis phase, hence suppressed the breast cancer progression. (ijpsonline.com)
Classic anti-cancer strategies aimed at actively killing cancer cells include alkylating agents that generate crosslinks in DNA and trigger repair mechanisms to induce apoptosis, antimetabolites that severely disrupt nucleic acid synthesis, and alkaloids that interfere with microtubule polymerization/depolymerization and halt mitosis 2 . (cancerbiomed.org)

The xenograft model in nude mice was used to elucidate the function of CMTM4 in tumourigenesis ex vivo. (biomedcentral.com)
Overexpression of CMTM4 also markedly inhibited the tumour xenograft growth in nude mice. (biomedcentral.com)
In this study, human tumor cells (NCI-H640, 1 × 10 7 in 100 μL) are implanted subcutaneously, or 1 × 10 7 in 50 μL in the thoracic cavity, in athymic nude mice (nu/nu). (wjtcm.net)
We successfully established subcutaneous and thoracic xenografts with human nonsmall cell lung cancer cell line xenografts in athymic nude mice in only 10 days. (wjtcm.net)
The antitumor activity and survival analysis of VALD-3 in vivo was determined by the nude mice xenograft assay. (researchsquare.com)
By xenografting human breast cancer cells into nude mice, we found that VALD-3 significantly suppressed tumor cell growth while showing low toxicity against major organs. (researchsquare.com)
In vivo studies in nude mice also confirms the same. (pka-signal.com)
Furthermore, in nude mice model, restored expression of CHFR by demethylation inhibited tumor growth and decreased sensitivity to paclitaxel. (bmj.com)

This gene is thought to regulate cell cycle progression. (wikipedia.org)
In different assays and cell types, overexpression of this protein has been shown to activate or suppress cell cycle progression. (wikipedia.org)
Some studies showed that blocking cell cycle progression ameliorated fibrosis. (nature.com)
The effects of CMTM4 on cellular apoptosis and cell cycle progression were analysed by flow cytometry, and cell cycle hallmarks were detected by western blotting and RT-PCR. (biomedcentral.com)
PP2A is involved in diverse regulatory functions, including cell cycle progression, apoptosis, and DNA repair. (cancerbiomed.org)
PP2A, a serine/threonine phosphatase composed of 3 distinct subunits-65-kDa PP2A-A (scaffold subunit), 55-kDa PP2A-B (regulatory subunit), and 37-kDa PP2A-C (catalytic subunit)-constitutes 0.2%-1.0% of the total protein content in mammalian cells and has been implicated in the regulation of diverse cellular processes, including signal transduction, cell cycle progression, DNA replication, gene transcription, and protein translation 5 - 8 . (cancerbiomed.org)
As a result, changes in SA-ß-Gal expression, cell cycle progression, and mitochondrial membrane potential were observed. (dojindo.com)
Common detection indicators for assessing cellular senescence include features related to cell cycle progression (DNA synthesis, p16/p21 expression, etc.), features related to morphology (of the cell, nucleus, nucleolus, etc. (dojindo.com)
Progression through the cell cycle is driven by the oscillating activity of Cyclin Dependent Kinases (CDKs). (intechopen.com)

The results showed that chrysin inhibited cancer cell growth at a dose-dependent manner by inducing apoptosis and cell cycle arrest at G2/M phase. (propolisscience.org)
This study is the first to show that VALD-3 induces apoptosis and cell cycle arrest in human breast cancer cells by suppressing Wnt/β-catenin signaling, indicating that it could be a potential drug for the treatment of breast cancer. (researchsquare.com)

This study aimed to investigate the effects of xanthoxyletin, a plant-derived coumarin, on human oral squamous cancer cells in vitro and in mouse xenografts in vivo . (medscimonit.com)
Mouse xenografts were used for the in vivo evaluation of the effects of xanthoxyletin. (medscimonit.com)
Tubeimoside I inhibits LPS-stimulated production of TNF-α, IL-1β, and IL-6, preventing the development of pulmonary edema and infiltration of inflammatory cells in vivo. (lktlabs.com)
In vivo, rigosertib treatment resulted in decreased tumor growth and increased survival of PDX mice. (lu.se)
Our research highlighted the toxic effect of TiO 2 NPs on corneas in vitro and in vivo , providing an alternative insight into TiO 2 NPs-induced corneal endothelial cell injury. (aging-us.com)
Chrysin, a natural and biologically active flavonoid suppresses tumor growth of mouse B16F10 melanoma cells: In vitro and In vivo study. (propolisscience.org)
The current report is aimed at finding out whether the antitumor potential of chrysin, evidenced in vitro and in vivo, is linked or not to its effect on immunological mechanisms of melanoma-bearing mice. (propolisscience.org)
In vivo, BALB/c mice received a subcutaneous injection of B16F10 melanoma cells prior to antitumor treatments with chrysin (50 mg/kg b.w) for 14 days and 21 days. (propolisscience.org)
These findings establish a mechanism for co-targeting DNA damage-induced cell cycle checkpoints in combination with repair of cisplatin-DNA lesions in vivo using RNAi nanocarriers, and motivate further exploration of ASL as a generalized strategy to improve cancer treatment. (nature.com)
This was done in vitro as well as in vivo by establishing a xenograft model from a Kir6.2/SUR2 positive high grade serous ovarian cancer cell line. (survivornet.com)
Importantly, depletion of H2A.Z isoforms reduces the tumor size in a mouse xenograft model in vivo and sensitizes PDAC cells to gemcitabine. (irpa2006europe.com)

little attention has been devoted to the mechanisms and other immune cells. (hindawi.com)
Although the use of anti-VISTA monoclonal antibody has demonstrated encouraging outcomes in the therapy of various malignancies, its specific impact and underlying mechanisms in oral squamous cell carcinoma (OSCC) remain to be explored. (bvsalud.org)
In this study, we investigated the cytotoxic and apoptotic effects of VALD-3, a Schiff base ligand synthesized from o-vanillin derivatives, on human breast cancer cells and the possible underlying mechanisms. (researchsquare.com)
In order to explore the underlying mechanisms, we performed tests to assess cell migration and motility, apoptosis, expression of glucose transporter 1 and proapoptotic proteins. (oncotarget.com)
Increased intracellular calcium disrupts mechanisms of cell division by arresting the cell cycle in G2/M phase and this is associated with alteration of the oxidative state, disruption of the mitochondria and activation of the caspase-3-independent cell death pathway. (survivornet.com)
Mechanisms regulating cardiomyocyte cell cycle arrest are of great interest partly because reversing this process could provide a way to stimulate cardiac regeneration after injury [ 17 ]. (springer.com)

In addition, Plk1 also prevented partial epithelial-mesenchymal transition (pEMT) of tubular epithelial cells via autophagy pathway. (nature.com)
In this study, we explored the role of Plk1 in the classical kidney fibrosis model, UUO, and cultured fibroblast and renal tubular epithelial cells by using specific Plk1 inhibitors, genetic silencing, and heterozygous global knockout mice. (nature.com)
The study included SCC-1 human oral cancer cells and EBTr normal embryonic bovine tracheal epithelial cells, which were treated with 0 µM, 5 µM, 10 µM, and 20 µM of xanthoxyletin for 24 hours. (medscimonit.com)
mmc9.pdf (66K) GUID:?55F9BD4F-0F85-40B3-99F0-75E20D3D96BC Supplemental Physique?S10 Silencing p21 reversed cell cycle G2/M arrest of TGF-1Ctreated proximal tubular epithelial cells. (holyexperiment.org)
We investigated the role of CAL-101 (GS-1101, Idelalisib) TGF-1 in inducing profibrotic signaling from epithelial cells to activate pericyte-myofibroblast transition. (holyexperiment.org)
8 Astrocytes interact with blood vessels, pericytes, and epithelial cells to organize CNS flow and modulate the blood-brain barrier (BBB). (news-medical.net)
Human bronchial epithelial cells were exposed to the nine CNT/F (0-24g/ml) to determine cell viability, inflammation, cellular oxidative stress, micronuclei formation, and DNA double-strand breakage. (cdc.gov)
All CNT/F induced some level of genotoxicity in human epithelial cells. (cdc.gov)
Our aim was to measure the genotoxicity of MWCNT-7 compared to these two physicochemically-altered MWCNTs in human lung epithelial cells (BEAS-2B & SAEC). (cdc.gov)

It induced apoptosis, suppressed cell migration and invasion, blocked cell cycle and phosphatidylinositol 3-kinase/ protein kinase B signaling pathway. (ijpsonline.com)
Intrinsic apoptotic pathway and G2/M cell cycle arrest involved in tubeimoside I-induced EC109 cell death. (lktlabs.com)
In the current study, we explored the effect of Nrf2 signaling pathway in TiO 2 NPs-induced corneal endothelial cell injury. (aging-us.com)
Further investigation suggested that Nrf2 signaling pathway activation and the downregulation of ZO-1, β-catenin and Na-K-ATPase were involved in TiO 2 NPs-induced mouse primary corneal endothelial cell injury. (aging-us.com)
VALD-3 also regulated the Wnt/β-catenin signaling pathway in breast cancer cells, inhibiting the activation of downstream molecules. (researchsquare.com)
Therefore, compared to normal p53-proficient cells, p53-defective cells are more reliant on MK2 activity, which drives an alternative cell cycle checkpoint pathway that stabilizes the CKI inhibitors p27 Kip1 and Gadd45α in order to maintain G 1 /S and G 2 /M arrest after certain types of DNA damage 16 , 18 . (nature.com)

The MTT assay assessed cell viability, and autophagy was detected by electron microscopy. (medscimonit.com)
The most promising candidate, CYT997, was tested for its invitro effects on cell viability and cell death, colony formation, cell cycle changes, and cell migration/invasion in HCC cells. (stanford.edu)
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay was executed for determination of effects of piperine on breast cancer cell viability. (ijpsonline.com)
The inhibitors demonstrated promising effects in vitro with reduced viability, increased differentiation and cell death of neuroblastoma cells. (lu.se)
Rigosertib reduced cell viability of neuroblastoma cells and caused cell cycle arrest in the G2/M phase. (lu.se)
Cell number and viability were assessed on harvested cells using the trypan blue dye exclusion assay. (moestuininfo.com)

Invivo, CYT997 (20mg/kg, three times weekly by oral gavage) significantly inhibited PDX growth, while being non-toxic to mice. (stanford.edu)
in other, similar cellular and animal models, this compound inhibits cell and tumor growth. (lktlabs.com)
Melatonin inhibited cell growth and induced apoptosis in 5RP7 cells. (tubitak.gov.tr)
This trial will combine two compounds that aim to starve the tumors while simultaneously blocking cancer cell growth. (med-chemist.com)
Cell Counting Kit-8 (CCK-8) and viable cell counting assays were used to delineate the growth curves of 786-O cells after CMTM4 overexpression or knockdown. (biomedcentral.com)
Restoration of CMTM4 significantly suppressed 786-O cell growth by inducing G2/M cell cycle arrest and p21 upregulation, and cell migration was also inhibited. (biomedcentral.com)
Evaluation of arrest of tumor growth was evaluated in a previous study. (survivornet.com)
This article discusses how a combination of live-cell analysis methods deliver the required flexibility to allow these highly dynamic astrocytic models to be characterized, via quantification of their growth, motility, morphology, and functional analysis. (news-medical.net)
The Incucyte ® Live-Cell Analysis System delivers flexible kinetic quantification of biological phenotypes to monitor growth, motility, morphology, and cellular function. (news-medical.net)
Temporal monitoring of brain region astroglia revealed differences in cell growth and morphology. (news-medical.net)
C1632 suppresses the growth of A549R xenograft tumours in miceThe above benefits prompted us to examine the endogenous antitumour activity of C1632 on A549R xenograft tumours in mice. (emlinhibitor.com)
p values have been calculated working with the unpaired Student's t test ( p 0.001)addition, C1632 suppressed the growth of xenograft tumour cells in a time- dependent manner (Figure 7C). (emlinhibitor.com)
11 Kosaka K, Yokoi T. Carnosic acid, a component of rosemary ( Rosmarinus officinalis L.), promotes synthesis of nerve growth factor in T98 G human glioblastoma cells. (thieme-connect.com)

In the present study, cytotoxic and apoptotic effects of melatonin were investigated on H-ras oncogene transformed rat embryo fibroblast 5RP7 cells. (tubitak.gov.tr)
These findings show that melatonin induces cell death and apoptosis in H-ras oncogene transformed 5RP7 cells. (tubitak.gov.tr)
Melatonin may be an anticancer agent against H-ras oncogene activated cancer cells. (tubitak.gov.tr)
Here, we report that in T cell acute lymphoblastic leukemia (T-ALL) the MYC oncogene controls the expression of TET1 and TET2 to maintain 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) patterns, which is associated with tumor cell-specific gene expression. (biomedcentral.com)

8 Visanji J M, Thompson D G, Padfield P J. Induction of G2/M phase cell cycle arrest by carnosol and carnosic acid is associated with alteration of cyclin A and cyclin B1 levels. (thieme-connect.com)

Regulator of cell cycle RGCC (RGCC) also known as response gene to complement 32 protein (RGC-32) is a protein that in humans is encoded by the RGCC gene. (wikipedia.org)
The protein also translocates to the nucleus in response to treatment with complement system proteins, and can associate with and increase the kinase activity of cell division cycle 2 protein. (wikipedia.org)
The human hemopoietic cell kinase (HCK) is a member of the src family of protein tyrosine kinases specifically expressed in myeloid cells and to a minor extent in B-lymphoid cells. (uni-frankfurt.de)
Electrophoretic mobility shift analysis using nuclear extracts obtained from RAW264 cells and from the promonocytic cell line U-937 revealed the formation of at least three distinct protein-DNA complexes at each of these sites, one of which was found to contain the transcription factor Sp1. (uni-frankfurt.de)
Our data reveal consistent ENKUR downregulation at both RNA and protein levels across MPN patient specimens and experimental models, with a concomitant upregulation of a cell cycle marker, CDC20. (stanford.edu)
Western blotting assay was implemented to check the levels of phosphatidylinositol 3-kinase and protein kinase B. Results revealed that piperine induced dose reliant cytotoxicity in MCF-7 human breast cancer cells. (ijpsonline.com)
and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)
The precise regulations of pre-RC protein levels and assembly are effective ways to prevent reassembly of de novo MCM2-7 onto the replicated origins to re-license and re-replicate the genomic DNA in the subsequent phases of the same cell cycle ( Figure 1) . (intechopen.com)
In the zebrafish, rprma mRNA is expressed in the olfactory placodes (OP) and olfactory epithelium (OE), rprmb is observed in the tectum opticum (TeO) and trigeminal ganglion (Tg), whereas rprml is found primarily in the telencephalon (Tel). At protein level, RPRM is present in a subset of cells in the OP, and neurons in the OE, TeO, hindbrain and sensory peripheral structures. (frontiersin.org)
Open in a separate window Fig. 1 The three H2A.Z isoforms are overexpressed in PDAC cell lines and in PDAC patients.a Histone-enriched protein extracts Deoxycholic acid from PDAC cell lines and the non-cancerous hTERT-HPNE cell line were analyzed by western blot using an anti-H2A.Z antibody. (irpa2006europe.com)
ATP-binding cassette (ABC) drug transporter ABCG2, one of multidrug resistance (MDR) protein which can effectively discharge a wide spectrum of chemotherapeutic agents out of cancer cells and subsequently reduce the intracellular concentration of these drugs. (pka-signal.com)
mRNA and protein expression of ABCG2 and phosphorylation of NF-κB was significantly higher in OXA-R than parental cells. (pka-signal.com)

Furthermore, MP increased WBC and HGB counts and reduced the ratio of G0/G1 phase obviously, increased the proportion of S phase and G2/M phase in peripheral blood lymphocytes, increased the counts of CD4+ T cells and the ratio of CD4+/CD8+, and reduced the inhibition rate of splenic lymphocytes. (hindawi.com)
Furthermore, we observed that inhibition of RUNX1/ETO in Kasumi1 cells and in RUNX1/ETO positive primary acute myeloid leukemia patient samples leads to up-regulation of miR144/451. (uni-frankfurt.de)
SL originally described a relationship between two genes, where alteration of either gene alone results in viable cells, but alteration (mutation, loss, or inhibition) of both genes simultaneously was lethal. (nature.com)

The percentages of early/late apoptotic cells to which melatonin (IC50: 380 μM) was administered were increased 6.2-fold in 5RP7 cells compared to controls for 48 h. (tubitak.gov.tr)
Chrysin-treated B16F10 cells were analyzed for their metabolic rate and apoptotic potentials. (propolisscience.org)
The expression of pro-apoptotic proteins and anti-apoptotic proteins in MCF-7 and MDA-MB-231 cells was investigated by Western blotting. (researchsquare.com)

Muse flow cytometric analysis was performed for assessing of different cell cycle phases. (ijpsonline.com)
The cell cycle arrest caused by melatonin was observed in G1 and G2 phases in the cell cycle analyses in 5RP7 cells for 48 h. (tubitak.gov.tr)

Combining some of these neoadjuvant approaches with standard-of-care chemotherapy or radiotherapy appears to "sensitize" tumor cells to treatment. (cancerbiomed.org)
5) increasing the intrinsic radioresistance of normal cells through ketone bodies but decreasing that of tumor cells by targeting glycolysis. (springer.com)
Tumor cells were cultured in the presence of increasing concentrations of Alizarin for 24, 48, 72, and 144 h. (moestuininfo.com)
While aberrant DNA methylation is a characteristic feature of tumor cells, our knowledge of how these DNA methylation patterns are established and maintained is limited. (biomedcentral.com)

V-domain Ig suppressor of T-cell activation (VISTA) is a novel immune checkpoint regulator that can inhibit T cell-mediated antitumor immunity. (bvsalud.org)
A slightly higher induction of average gene expression was observed at the G2-M checkpoint in Xpc-/- mice, but this did not reach statistical significance (P=0.086). (maastrichtuniversity.nl)
In response to DNA damage, a synthetic lethal relationship exists between the cell cycle checkpoint kinase MK2 and the tumor suppressor p53. (nature.com)
Impairment of a cell cycle checkpoint is often associated with sensitivity to chemotherapeutic drugs. (bmj.com)
Here, we studied the correlations between the checkpoint with forkhead-associated and ring finger (CHFR) gene expression and responses to paclitaxel in endometrial cancer cells. (bmj.com)

In CKD patients or obstructive mice model, we observed that Plk1, a critical mitotic regulator, was upregulated in kidneys. (nature.com)
Treatment of neuroblastoma PDX cells with ARRY-520 resulted in mitotic arrest and subsequent cell death. (lu.se)
Recent breakthroughs have uncovered more and more DNA replication licensing machinery proteins (ORC, Cdc6, Cdt1, geminin, etc.) functioning in other cell cycle events, including centrosome replication, mitotic events, transcription and so on. (intechopen.com)

The risk is increased 1000-fold for squamous cell carcinoma, basal cell carcinoma, malignant melanoma, and fibrosarcoma and is increased 10-fold to 20-fold for other tumors. (medscape.com)

ABSTRACT: BACKGROUND: Benzo[a]pyrene (B[a]P) exposure induces DNA adducts at all stages of spermatogenesis and in testis, and removal of these lesions is less efficient in nucleotide excision repair deficient Xpc-/- mice than in wild type mice. (maastrichtuniversity.nl)

Clonogenic assay was implemented for checking the effect of piperine on clonogenic potency of breast cancer cells. (ijpsonline.com)
Cell migration and invasion was monitored by transwell chambers assay. (ijpsonline.com)
Acridine orange/ethidium bromine staining assay revealed that piperine induced apoptosis in MCF-7 cells which was further investigated by Western blotting. (ijpsonline.com)
On top of that, an Edu staining assay and flow cytometry have been performed to additional investigate regardless of whether C1632 inhibited the colony formation of A549 and/or A549R cells by DNA replication inhibitionand cell cycle arrest. (emlinhibitor.com)
C) C1632 inhibits migration of A549R cells inside the scratch-wound healing assay. (emlinhibitor.com)
D) Quantification of the results in (C). (E) C1632 inhibits migration and invasion of A549R cells in the Transwell assay. (emlinhibitor.com)

1632 remedy, even at a higher dose, did not drastically enhance apoptosis or senescence (Figures S8 and S9), suggesting that the inhibitory effects of C1632 on colony formation are not because of cytotoxicity-induced cell death. (emlinhibitor.com)
We cultured 6 endometrial cancer cell lines exposed to paclitaxel, studied the cell cytotoxicity, cell cycle distribution, CHFR expression, and methylation status before and after a demethylation agent (5-aza) treatment. (bmj.com)

Senescent cells elicit their fibrogenic actions primarily by secreting an assortment of inflammatory and profibrotic factors known as the senescence-associated secretory phenotype (SASP). (frontiersin.org)
Increasing evidence indicates that senescent cells could be a promising new target for therapeutic intervention known as senotherapy, which includes depleting senescent cells, modulating SASP and restoration of senescence inhibitors. (frontiersin.org)
We also highlight potential options of targeting senescent cells for the treatment of CKD. (frontiersin.org)
We also highlight potential options for targeting senescent cells in developing therapeutics for CKD patients. (frontiersin.org)
Knockdown of these H2A.Z Deoxycholic acid isoforms in PDAC cell lines induces a senescent phenotype, cell cycle arrest in phase G2/M, increased expression of cyclin-dependent kinase inhibitor CDKN2A/p16, SA-galactosidase activity and interleukin 8 production. (irpa2006europe.com)
Scientists have discovered that, in the absence of resident stem cells, senescent cells can instruct neighboring somatic cells to reprogram. (dojindo.com)
None of the individual biomarkers that have been identified so far have been deemed to be specific to senescent cells. (dojindo.com)
The development of drugs that eliminate senescent cells in the body (senolytic drugs) is also attracting the attention of researchers as a possible strategy to extend healthy life expectancy. (dojindo.com)

The melatonin treatment resulted in increased caspase-3 activity and reduced the mitochondrial membrane potential in the 5RP7 cells for 48 h. (tubitak.gov.tr)

It is involved in p53 pathways and is implicated in cell death/survival signaling, the cell cycle, and differentiation, thereby playing a regulatory role in carcinogenesis [ 12 ]. (hindawi.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Transcriptome analysis of H2A.Z-depleted PDAC cells showed altered gene expression in fatty acid biosynthesis pathways and those that regulate cell cycle and DNA damage repair. (irpa2006europe.com)
Role of ABCG2 and plausible molecular signaling pathways involved in Oxaliplatin-Resistant (OXA-R) colon cancer cells was evaluated in the present study. (pka-signal.com)
These marker proteins are known to be tumor suppressors and regulate the cell cycle mainly through two pathways (p16Ink4a-RB and p53-p21CIP1). (dojindo.com)

Abstract: A network of lineage-specific transcription factors and microRNAs tightly regulates differentiation of hematopoietic stem cells along the distinct lineages. (uni-frankfurt.de)
This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. (cancerindex.org)

This revealed increased expression of B-cell lymphoma 2 associated X and reduced expression of B-cell lymphoma 2, indicating apoptosis induction by piperine. (ijpsonline.com)

Cell apoptosis was investigated using 4',6-diamidino-2-phenylindole (DAPI), annexin V, and propidium iodide (PI) fluorescence flow cytometry, which was also used to investigate the cell cycle. (medscimonit.com)
Flow cytometry analysis showed that VALD-3 triggered cell cycle arrest and induced apoptosis of breast cancer cells. (researchsquare.com)

In addition, analysis of cell cycle phase dependent gene expression revealed that expression of genes involved in G1-S and G2-M phase arrest was increased after B[a]P exposure in both genotypes. (maastrichtuniversity.nl)
CONCLUSIONS: Gene expression in testis of untreated Xpc-/- and wild type mice were very similar, with only 4 genes differentially expressed. (maastrichtuniversity.nl)

We used an HCC patient-derived xenograft (PDX) mouse model to assess its invivo efficacy.RESULTS: CYT997 was significantly more cytotoxic against HCC cells than against primary human hepatocytes, and sensitized HCC cells to sorafenib. (stanford.edu)
For this work, I have used patient-derived xenograft mouse models and neuroblastoma cells grown as 3D tumor organoids. (lu.se)

OXA treatment resulted in G2 phase arrest in parental LoVo cells, which was overcome by OXA-R LoVo cells. (pka-signal.com)

B: Silencing p21 reversed cell cycle G2/M arrest of TGF-1Ctreated PTECs. (holyexperiment.org)

Cell migration as well as cell invasive ability of MCF-7 cells was reduced to minimum by the application of piperine in dose dependent-manner. (ijpsonline.com)
OXA resistant LoVo cells was developed by exposing the colon cells to OXA in a dose-dependent manner. (pka-signal.com)
RESULTS: Dose-dependent partitioning of individual nanotubes in the cell nuclei was observed for each MWCNT material and was greatest for MWCNT-7. (cdc.gov)

10 Huang S C, Ho C T, Lin-Shiau S Y, Lin J K. Carnosol inhibits the invasion of B16/F10 mouse melanoma cells by suppressing metalloproteinase-9 through down-regulating nuclear factor-kappa B and c-jun. (thieme-connect.com)

Several in vitro studies have demonstrated that artemisinin and its derivatives possess promising anticancer, antiproliferative, and antiapoptotic properties in tumor cell lines of colon, breast, and lung. (wjtcm.net)
Rat primary astrocytes were isolated from different brain regions (cortex, cerebellum, and hippocampus) and grown in vitro to demonstrate the utilization of live-cell analysis for monitoring and comparing the diversity of cultured astroglia phenotypes. (news-medical.net)
Interestingly, in vitro experiments indicated that not only splenic cells but also cells in the peritoneal cavity induced anti-PEG IgM following incubation with PEG-Lip. (matci.jp)

We injected 4T1 cells into 50 Balb/c mice to generate tumor xenografts. (bioone.org)

Melatonin was not toxic in NIH/3T3 primary mouse embryonic fibroblast cells at low doses for 24 and 48 h. (tubitak.gov.tr)
Results PR-SET7 deficiency in embryonic hepatocytes impairs liver?organogenesis Mice carrying hepatocyte-specific deletion of in embryonic liver were generated by crossing mice (Oda mice. (antiviralbiologic.com)
Open in a separate window Figure 1 PR-SET7 is required for proper liver organogenesis during embryonic development A Representative pictures of embryos at 18.5?days postcoitum (E18.5) and hematoxylin and eosin staining of whole-mount embryo sections from mice and control littermates (and mRNA levels. (antiviralbiologic.com)

Cell apoptosis and senescence-like morphology were also observed. (stanford.edu)
To monitor morphology of cancerous breast cells, phase contrast microscopy was performed after piperine exposure. (ijpsonline.com)

The present study showed that Polo-like kinase 1 (Plk1) was upregulated in the kidneys of CKD patients and mice subjected to unilateral ureteral obstruction (UUO) with location in proximal tubules and tubulointerstitial fibroblasts. (nature.com)

Due to its key role in the eukaryotic cell cycles, Plk1 has been one of the most validated drug targets for cancer treatment. (nature.com)
Further, application of piperine on cancer cell colonies remarkably reduced the number of cell colonies to minimum. (ijpsonline.com)
In conclusion, the current investigation regarding anticancer effects of piperine against breast cancer revealed remarkable suppression of cancer cells. (ijpsonline.com)
Cancer Research UK's Drug Development Office has opened the first trial of a new drug combination in patients with advanced solid tumors and in a subset of patients who have non-small cell lung cancer. (med-chemist.com)
In the second stage patients with non-small cell lung cancer (NSCLC) will receive the combination. (med-chemist.com)
From this preliminary study, it appears that combination of dimer oxime and topotecan may be used as chemotherapeutic agents against nonsmall cell lung cancer. (wjtcm.net)
[3] In a recent study, the antitumor activity of five chemically synthesized dihydroartemisinin dimers against human colon cancer cell lines, HT29, HCT116 and the nontumorigenic colon cell line, and FHC were compared. (wjtcm.net)
Here, we investigated a radioiodinated PARP inhibitor, [ 125 I]KX1, and show drug target specific DNA damage and subsequent killing of BRCA1 and non-BRCA mutant ovarian cancer cells at sub-pharmacological concentrations several orders of magnitude lower than traditional PARP inhibitors. (osti.gov)
Finally, we showed tumors can be imaged by single-photon computed tomography (SPECT) with PARP theranostic, [ 123 I]KX1, in a human ovarian cancer xenograft mouse model. (osti.gov)
Preclinical success has led to a profusion of clinical trials on LB100 adjuvant therapies, including a phase I trial in extensive-stage small-cell lung cancer, a phase I/II trial in myelodysplastic syndrome, a phase II trial in recurrent glioblastoma, and a completed phase I trial assessing the safety of LB100 and docetaxel in various relapsed solid tumors. (cancerbiomed.org)
Because BRCA mutations are observed in fewer than 10% of cancer patients (cBioPortal: 6.7%) 11 , 12 , 13 the identification of additional genes that share synthetic lethal sensitivity relationships with mutated oncogenes or tumor suppressors would greatly enhance the implementation of tumor cell-specific synthetic lethal sensitivity to improve an anticancer therapeutic response. (nature.com)
Cancer cells that are defective in p53 function are deficient in their ability to transcriptionally upregulate the CDK inhibitor p21 after genotoxic stress. (nature.com)
Two novel compounds labelled as thioglycoside A and B were designed and evaluated on breast and colon cancer cell lines. (oncotarget.com)
We assessed their cytotoxic effect after sensitizing cancer cells with insulin. (oncotarget.com)
Conjugation of glucose with metabolic agents to selectively target cancer cells was inspired by the widespread use of radiolabeled glucose analog to visualize tumors and their metastases. (oncotarget.com)
We conducted a systematic review of the literature on the effects of vitamin D on ovarian cancer cell. (mdpi.com)
The osteosarcoma cells appeared to be the most sensitive, whereas the prostate cancer cells was substantially unaffected. (moestuininfo.com)
Consistent with this hypothesis, prostate cancer cells appeared to be resistant to Alizarin treatment. (moestuininfo.com)
Two weeks right after injection using the cancer cell inoculum, and after that every single two days thereafter, mice have been injected within the caudal vein with 30 mg/kg C1632. (emlinhibitor.com)
Cancer cells acquires resistance to anti-cancer drug and develops resistance. (pka-signal.com)
The intra strand cross-links formed by OXA being the most abundant lesions capable of blocking both replication and transcription of DNA, they are considered to cause the major cytotoxic lesions and being directly involved in the cancer cells death (Woynarowski et al. (pka-signal.com)
Our data suggest that CHFR suppression regulated by hypermethylation may sensitize endometrial cancer cells to paclitaxel, and CHFR may be a promising marker to predict the response of endometrial cancer to paclitaxel. (bmj.com)
Our results demonstrate that in these mice, hepatocyte death initially leads to the activation of ductal progenitors and inflammation, followed by spontaneous development of hepatocellular carcinoma comprised mainly of cells featuring cancer stem cell properties. (antiviralbiologic.com)
Recently, various fields have given particular attention to cellular senescence due to the recent discoveries of SASP (a known cancer-causing factor) and senescence-related phenomena in stem cell research. (dojindo.com)

In this chapter, we mainly discuss the coordination regulations between DNA replication initiation and other cell cycle events that ensure genomic integrity. (intechopen.com)
DNA replication occurs once and only once per cell cycle mainly regulated by DNA replication initiation factors in eukaryotic cells. (intechopen.com)

Wound healing and transwell assays were performed to assess the cells' ability to migrate. (biomedcentral.com)
A) Representative photos of FAK in C1632-treated and untreated A549R cells in immunofluorescence assays. (emlinhibitor.com)

HCK expression is up-regulated at the transcriptional level during myeloid differentiation of hematopoietic cells. (uni-frankfurt.de)

OXA-R LoVo cells exposed to NF-κB inhibitor QNZ effectively reduced the ABCG2 and p-NF-κB expression and increased ER stress marker expression. (pka-signal.com)
Metastasis marker proteins also downregulated in OXA-R cells. (pka-signal.com)
In line with this, the vast majority of A6-positive cells were co-stained with the hepatocyte marker HNF4 (Fig? (antiviralbiologic.com)

Aging kidney and CKD share many common characteristic features with increased cellular senescence, a conserved program characterized by an irreversible cell cycle arrest with altered transcriptome and secretome. (frontiersin.org)
Cellular senescence is characterized by an irreversible and permanent cell cycle arrest coupled with altered transcriptome and secretome. (frontiersin.org)
Irreversible cell cycle arrest is one of the phenomena that characterize cellular senescence. (dojindo.com)
Cellular senescence is controlled by various factors such as cell type and physiological conditions, such as oxidative stress. (dojindo.com)
Research into apoptosis, necrosis, autophagy, and cellular senescence is very important for understanding the intracellular functions that control cell survival and death. (dojindo.com)

Renal cell carcinoma (RCC) incidence has increased over the past two decades. (hindawi.com)
Renal cell carcinoma (RCC) accounts for approximately 3% of human malignancies, and its incidence appears to be increasing globally [ 1 ]. (hindawi.com)
Primary clear cell renal cell carcinoma (ccRCC) and the paired adjacent non-tumour tissues were then collected to examine the expression of CMTM4 by western blotting, immunohistochemistry, and quantitative RT-PCR. (biomedcentral.com)

BC comprises of variant subtypes and those subtypes arise from basal progenitor or luminal progenitor cells undergoing different genetic mutations[ 2 ]. (ijpsonline.com)

Astrocytes are a heterogeneous population of cells with distinguishing functional and morphological characteristics and are specialized to their different brain regions and locations. (news-medical.net)
Fig6CCE),6CCE), suggesting that they are derived from a heterogeneous population of cells. (antiviralbiologic.com)

Anatomy28

Organisms16

Diseases5

Chemicals and Drugs108

Analytical, Diagnostic and Therapeutic Techniques and Equipment18

Phenomena and Processes83

Disciplines and Occupations1

Information Science3

Health Care2

Promoted apoptosis and cell cycle1

Proliferation20

Mitosis3

Nude mice8

Progression9

Apoptosis and cell cycle arrest2

Vivo11

Mechanisms6

Epithelial cells9

Pathway6

Viability6

Growth13

Oncogene4

Phase cell cycle arrest1

Protein12

Inhibition3

Apoptotic3

Phases2

Tumor cells4

Checkpoint5

Mitotic3

Squamous1

NUCLEOTIDE EXCISI1

Assay6

Cytotoxicity2

Senescent8

Mitochondrial membrane potential1

Pathways5

Regulates2

Induction1

Flow cytometry2

Gene expression2

Patient-derived xenograft2

Treatment resulted1

Silencing1

Dose-dependent3

Melanoma cells1

Vitro3

Xenografts1

Embryonic3

Morphology2

Fibroblasts1

Cancer24

Replication2

Assays2

Differentiation1

Marker3

Cellular5

Renal Cell Carc3

Progenitor cells1

Heterogeneous population of cells2