AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation Science
Proliferating Cell Nuclear AntigenCell CycleCell ProliferationCell Cycle ProteinsApoptosisCell Cycle CheckpointsCell DivisionG1 PhaseCell Line, TumorMitosisSignal TransductionS PhaseCells, CulturedCyclinsTumor Suppressor Protein p53Cell SurvivalCell LineCyclin-Dependent Kinase Inhibitor p21G2 PhasePhosphorylationFlow CytometryCaspasesTumor Cells, CulturedCyclin-Dependent Kinase Inhibitor p27Caspase 3Apoptosis Regulatory ProteinsProto-Oncogene Proteins c-bcl-2Retinoblastoma ProteinMenstrual CycleCell DifferentiationProtein-Serine-Threonine KinasesBlotting, WesternRNA, MessengerG2 Phase Cell Cycle CheckpointsDNA-Binding ProteinsCyclin D1Inhibitor of Apoptosis ProteinsTime FactorsG0 PhaseMolecular Sequence DataDNA DamageGene Expression RegulationG1 Phase Cell Cycle CheckpointsTranscription FactorsNuclear ProteinsDown-RegulationTumor Suppressor ProteinsTransfectionCyclin-Dependent Kinase 2Enzyme ActivationCDC2 Protein KinaseAntineoplastic AgentsRNA, Small InterferingMutationIn Situ Nick-End LabelingProto-Oncogene ProteinsBromodeoxyuridineCell NucleusDNA FragmentationCyclin EModels, BiologicalDNA ReplicationGene Expression Regulation, NeoplasticHeLa Cellsbcl-2-Associated X ProteinEnzyme InhibitorsCyclin AReverse Transcriptase Polymerase Chain ReactionFibroblastsDose-Response Relationship, DrugImmunohistochemistryDNACyclin-Dependent Kinase 4Genes, cdcBase SequenceInterphaseTranscription, GeneticCyclin BAmino Acid SequenceMice, Inbred C57BLUp-RegulationGene ExpressionRNA InterferenceE2F Transcription FactorsCDC2-CDC28 KinasesProtein BindingE2F1 Transcription FactorMice, KnockoutCarrier Proteinscdc25 PhosphatasesMitochondriaCyclin B1Cyclin DGene Expression ProfilingProto-Oncogene Proteins c-aktX-Linked Inhibitor of Apoptosis ProteinEstrous CycleProto-Oncogene Proteins c-mycEpithelial CellsCell Death
ViabilityDifferentiation and apoptosisArrestProgressionFlow cytometryAssaysAssayOxidative stressInductionInhibits proliferationAutophagySignificantlyAnnexin V-FITCInhibitMitochondrialCancerInvasionInflammationBrdUPathwaysVitroPhosphorylationMETHODSCyclin-dependMechanismsProteinsGenesVivoTransformationMechanismDose-dependentReagentsColony formationColorectalDendritic cellMetabolism and cellAntigenImmuneMacrophageCheckpointRegulation of cellDetectionAssess cellTumor cellSignaling PathwayKinaseGeneProgrammed cellProtein synthesisCytotoxicityTissue homeostasisAlterationHallmarks
Viability15
  • Pancreatic cancer cells (MIA PaCa-2, BxPC-3, and CFPAC-1) and non-tumorigenic pancreas cells (HPDE) were treated with oleuropein, hydroxytyrosol and tyrosol to determine their effect on cell viability. (uaeu.ac.ae)
  • Outcomes Leucine deprivation decreases viability of tumor cells We 1st determined the result of leucine deprivation on viability of tumor cells. (racetab.org)
  • Open up in another window Shape 1 The result of leucine deprivation on cell viability(A and B) Cells had been incubated in charge BAY 293 (+leu) or leucine-deficient (?leu) moderate for indicated period, accompanied by MTT assay. (racetab.org)
  • HL-60 cells incubated with various amounts (1, 3, 5, 7.5, and 10 micro l/mL) of the extracts for 1-3 days showed dose-dependent, time-dependent growth suppression and decrease in cell viability. (nih.gov)
  • The MTT assay assessed cell viability, and autophagy was detected by electron microscopy. (medscimonit.com)
  • Relative cell viability was assessed using MTT assays. (wjgnet.com)
  • Learn about cell viability assays. (abcam.com)
  • Co-inhibition of IGF and CHK1 caused synergistic suppression of cell viability, cell survival and tumour growth in 2D cell culture, 3D spheroid cultures and in vivo. (nature.com)
  • Taken together, our results suggested that decreased LAPTM5 inhibited proliferation and viability, as well as induced G0/G1 cell cycle arrest possibly via deactivation of ERK1/2 and p38 in BCa cells. (spandidos-publications.com)
  • Methods for detecting apoptosis or dead cells (viability) by cell preparation type. (bdbiosciences.com)
  • Cell viability was detected by the Cell Counting Kit-8 (CCK-8) assay. (hindawi.com)
  • ADH activity and ALDH expression were much lower in SNU449 compared with Huh-7 cells and at the 270 mM dose, CAE decreased cell viability by about 50% and 80%, respectively, in Huh-7 and SNU449 cells but induced mortality only in Huh-7 cells. (springer.com)
  • Cell viability was ascertained through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay after deployment of TO in different doses. (jcimjournal.com)
  • TO demonstrated a dose-dependent decrease in viability of A549 cells after 24 h of exposure. (jcimjournal.com)
  • Effects of Thuja occidentalis ethanolic extract on viability of A549 cell line (A) and L-132 cell line (B) Cells were exposed to Thuja occidentalis ethanolic extract for 24 h at different concentrations and the cell viability was determined by MTT assay. (jcimjournal.com)
Differentiation and apoptosis3
Arrest16
  • The inhibition of cell proliferation was related to arrest of the cell cycle in the G0/G1 phase and increased apoptosis in response to PTGR1 knockdown as indicated by flow cytometry. (go.jp)
  • These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
  • Methionine restriction can significantly inhibit the proliferation, promote cell cycle arrest and induce apoptosis of HL-60 and Jurkat cells , which suggests that methionine restriction may be a potential therapeutic strategy for acute leukemia . (bvsalud.org)
  • Flow cytometric analysis revealed partial cell arrest in the G(1) phase at less than 5 micro L/mL and induction of apoptosis at 10 micro L/mL or more of ethanol and water extracts, with the latter exhibiting more pronounced inhibition than the former. (nih.gov)
  • The inhibition of cell growth was also demonstrated by cell cycle arrest at G2/M phase. (greenmedinfo.com)
  • Moreover, it induced cell cycle arrest in the G0/G1 phase, apoptosis, and ROS generation in these cells. (jcancer.org)
  • Silibinin considerably reduced the development of oral cancer cells by inducing apoptosis, G 0 /G 1 arrest, ROS generation, and activation of the JNK/c-Jun pathway. (jcancer.org)
  • Moreover, cell cycle arrest at G0/G1 phase was triggered by decreased LAPTM5 as well, which could lead to delayed BCa cell growth. (spandidos-publications.com)
  • We show here that expression of EBF3 resulted in cell cycle arrest and apoptosis. (aacrjournals.org)
  • Pack LR, Daigh LH, Meyer T. Putting the brakes on the cell cycle: mechanisms of cellular growth arrest. (bdbiosciences.com)
  • Cd inhibited cell proliferation via cell cycle arrest and induced apoptosis in a dose-dependent manner. (cdc.gov)
  • Cd at 20 microM markedly increased cells in G2/M and hypodiploid sub-G1 phases of the cell cycle suggesting cycle arrest and cell death. (cdc.gov)
  • Inhibition of ERK suppressed DNA synthesis and had an additive effect with Cd-inhibited proliferation suggesting that Cd-induced ERK activity is not responsible for the G2/M arrest and subsequent inhibition of cell proliferation. (cdc.gov)
  • Results suggested that p53 is not involved in Cd-induced cell cycle arrest and apoptosis in J774A.1 cells. (cdc.gov)
  • 10 mg/mL) of Nidus Vespae decoction (NVD) inhibited SGC-7901 gastric cancer cell growth by inducing G2/M cell cycle arrest and apoptosis. (hindawi.com)
  • Furthermore, the supernatant of THP-1 DCs significantly inhibited the proliferation of gastric cancer cells by inducing apoptosis and G1/S cell cycle arrest. (hindawi.com)
Progression11
  • It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
  • Increased levels of POGLUT2 could result in the dysregulated immune cell infiltration and tumor microenvironment and showed a significant regulatory effect on the progression of breast cancer through Notch-related signaling. (frontiersin.org)
  • Investigating the mechanism of synthetic lethality, we reveal that CHK1 inhibition in IGF-1R depleted or inhibited cells further downregulated RRM2, reduced dNTP supply and profoundly delayed replication fork progression. (nature.com)
  • The reversible lysine acetylation of histone H3 may play a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair, nuclear import and neuronal repression. (abcam.com)
  • Cyclin-dependent kinase (CDK) 10, is reported to play an essential role in the progression from the G2 to M phase of the cell cycle. (jcancer.org)
  • Deregulation of cell cycle progression is considered to be critical for carcinogenesis ( 9 ). (jcancer.org)
  • A series of signal transduction events starting from the activation of cyclin-dependent kinase 2 (CDK2) to binding of E2F -target transcription to phosphorylation of Rb helps maintain cell-cycle progression. (bdbiosciences.com)
  • S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (cancerindex.org)
  • In normal cell cycles STAT3's activation is fleeting, but in many cancer cell lines aberrantly activated STAT3 promotes cancer progression. (hawaii.edu)
  • The first project focuses on E2F1, a regulator of genes important for cell cycle progression and apoptosis. (mdanderson.org)
  • A multitude of pathways control cyclin-dependent kinase activities as the major event for cell cycle progression. (benthamscience.com)
Flow cytometry8
Assays5
  • Furthermore, POGLUT2 knockdown of breast cancer cells was established, followed by in vitro biological function assays and in vivo tumor growth study. (frontiersin.org)
  • Learn about the assays used to assess cell proliferation and the cell cycle. (abcam.com)
  • The effects of silibinin on the migration and invasion of oral cancer cells were evaluated using transwell assays. (jcancer.org)
  • Choose from a variety of BD Biosciences assays and reagents for visualizing cellular events such as apoptosis, cell proliferation and the cell cycle. (bdbiosciences.com)
  • Effects of Thuja occidentalis ethanolic extract on A549 cell migration (Light microscopy, ×20) Wound-healing assays were performed to assess cell migration. (jcimjournal.com)
Assay7
  • Cell Counting Kit-8 ( CCK-8 ) assay was used to detect the effect of methionine restriction on HL-60 and Jurkat cells proliferation. (bvsalud.org)
  • The expression of cell cycle -related proteins cyclin B1 , CDC2 and apoptosis -related protein Bcl-2 was evaluated by Western blot assay. (bvsalud.org)
  • Open up in another window Shape 3 Leucine deprivation induces apoptosis of human being breast tumor BAY 293 cell(A and B) MDA-MB-231 or MCF-7 cells had been incubated in charge (+leu) or leucine-deficient (?leu) moderate for 48h, accompanied by dedication of cleaved caspases inside a and annexin-V assay in B. (C and D) Nude mice. (racetab.org)
  • Review our assay kits for the study of apoptosis. (abcam.com)
  • The cell survival rate was measured using the principle of water-soluble tetrazolium salt-1 assay, which is a measurement method for cell survival rate. (researchgate.net)
  • Senescence-associated β-galactosidase (SA-β-gal) assay was implemented to dye β-galactosidases (used as a cell aging biomarker) to measure HDF cell aging by UVA. (researchgate.net)
  • Effects of Thuja occidentalis ethanolic extract on cellular proliferation tested by BrdU incorporation assay Cells were exposed to three different concentration of Thuja occidentalis ethanolic extract for 6, 12 and 24 h along with incorporation of BrdU. (jcimjournal.com)
Oxidative stress2
  • These include regulation of cell cycle, activation of mitochondrial pathway, induction of mitochondrial outer membrane permeabilization, induction of apoptosis, modulation of oxidative stress, amelioration of inflammation, modulation of insulin signaling and inhibition of angiogenesis. (mdpi.com)
  • At the cellular and whole organ level, degenerative changes that are a hallmark of natural aging (shorter telomeres, increased expression of cellular senescence markers, increased DNA damage, oxidative stress, and apoptosis, accompanied by diminished elasticity) reach pathological levels in aging humans in the form of chronic respiratory disease. (karger.com)
Induction6
  • induction of apoptosis, and (3). (nih.gov)
  • I'm-Yunity acts selectively in HL-60 leukemic cells, resulting in cell cycle restriction through the G(1)/S checkpoint and the induction of apoptosis. (nih.gov)
  • Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
  • These findings indicate that sesamin inhibits cervical cancer cell proliferation, and its mechanism may be attributed to the induction of p53/PTEN-mediated apoptosis. (medsci.org)
  • However, the underlying mechanisms involved in the induction of genomic instability, genotoxicity, mutations and consequent increased cell proliferation are still a matter of intense research. (benthamscience.com)
  • The THP-1 human monocytic cell line was used as a source of monocytic effector cells for analyzing proliferation and dendritic cell (DC) induction. (hindawi.com)
Inhibits proliferation2
Autophagy4
  • Involvement of C/EBPβ-related signaling pathway in methamphetamine-induced neuronal autophagy and apoptosis. (cancerindex.org)
  • Our previous study has shown that CCAAT-enhancer binding protein β (C/EBPβ) is an important regulator in METH-induced neuronal autophagy and apoptosis. (cancerindex.org)
  • We hypothesized that C/EBPβ is involved in METH-induced DDIT4-mediated neuronal autophagy and Trib3-mediated neuronal apoptosis. (cancerindex.org)
  • We tested our hypothesis by examining the effects of silencing C/EBPβ, DDIT4, Trib3 or α-syn with small interfering ribonucleic acid (siRNA) on METH-induced autophagy and apoptosis in the human neuroblastoma SH-SY5Y cells. (cancerindex.org)
Significantly7
  • Functional analysis revealed that knockdown of PTGR1 significantly inhibited proliferation and colony formation by PC cells. (go.jp)
  • Furthermore, Western blot analysis demonstrated that methionine restriction significantly reduced the proteins expression of Cyclin B1 (P Jurkat cells . (bvsalud.org)
  • The growth rate of the cells was reduced when grown in ( significantly? (racetab.org)
  • Xanthoxyletin significantly inhibited the proliferation of oral cancer cells (IC₅₀, 10-30 µM) with lower cytotoxicity for normal cells. (medscimonit.com)
  • Furthermore, we established a BCa cell model with downregulated LAPTM5, revealing a significantly delayed growth rate in the BCa cells with knockdown of LAPTM5. (spandidos-publications.com)
  • Inhibition of p53 by pifithrin-α significantly reduced the levels of PUMA, Bax, and PTEN but restored AKT phosphorylation in SiHa cells exposed to sesamin. (medsci.org)
  • High-risk MDS cases had a significantly higher percentage of apoptotic CD34+ cells and anti-apoptotic survivin+ cells than controls, particularly for trisomy 8 cases. (who.int)
Annexin V-FITC3
  • Annexin V - FITC / PI double staining was applied to detect apoptosis of HL-60 and Jurkat cells following methionine restriction. (bvsalud.org)
  • Fluorescence-activated cell sorting analysis after annexin V-fluorescein isothiocyanate and propidium iodide (annexin V-FITC-PI) dual staining method was done to ascertain early stage of apoptosis, if any. (jcimjournal.com)
  • Annexin-V-FITC positivity of cells up to 11.72% as compared to the untreated control revealed early state of TO-induced apoptosis. (jcimjournal.com)
Inhibit2
  • The article also underscores the various possible mechanisms by which honey may inhibit growth and proliferation of tumors or cancers. (mdpi.com)
  • IM combined with IR can synergistically inhibit the proliferation, apoptosis, clonogenesis, invasion, migration and cell cycle of GIST cells. (researchsquare.com)
Mitochondrial5
  • At the cellular level, aging is marked by depletion of adult stem cell reservoirs, the inability to maintain baseline homeostasis, a reduced response to stress, an increased accumulation of damaged DNA leading to telomere shortening, and mitochondrial dysfunction [ 1,2 ]. (karger.com)
  • The Drosophila retinoblastoma protein, Rbf1, induces a debcl and drp1 -dependent mitochondrial apoptosis. (sdbonline.org)
  • This study demonstrates that debcl and the pro-fission gene drp1 are necessary downstream of buffy to trigger a mitochondrial fragmentation during rbf1 -induced apoptosis. (sdbonline.org)
  • Notably, Debcl modulates Drp1 mitochondrial localization during apoptosis. (sdbonline.org)
  • These results provide a mechanism by which Drosophila Bcl-2 family proteins can control apoptosis and shed light on a link between Rbf1 and mitochondrial dynamics, in vivo. (sdbonline.org)
Cancer35
  • Furthermore, di-GA inhibited the generation of lymphendothelial gaps by cancer cell spheroid-secreted lipoxygenase metabolites. (nature.com)
  • Cancer Cell. (wikipedia.org)
  • Therefore, the search for novel agents, which induce apoptosis in cancer cells while displaying limited toxicity towards normal cells, is paramount. (uaeu.ac.ae)
  • The olive biophenols, oleuropein, hydroxytyrosol and tyrosol, have displayed cytotoxicity towards cancer cells without affecting non-tumorigenic cells in cancers of the breast and prostate. (uaeu.ac.ae)
  • Four varieties of tumor cells were examined: malignant melanoma A375, lung caner A549, ovarian carcinoma breast and A2780 cancer MCF-7 and MDA-MB-231 cells. (racetab.org)
  • However, the mechanism(s) responsible for the effects of Ganoderma lucidum on cancer cells remain to be elucidated. (greenmedinfo.com)
  • We have previously demonstrated that Ganoderma lucidum down-regulated the expression of NF-kappaB-regulated urokinase plasminogen activator (uPA) and uPA receptor (uPAR), which resulted in suppression of cell migration of highly invasive human breast and prostate cancer cells. (greenmedinfo.com)
  • In this study, we investigated the effects of Ganoderma lucidum on cell proliferation, cell cycle, and apoptosis in human prostate cancer cells PC-3. (greenmedinfo.com)
  • Thus, Ganoderma lucidum exerts its effect on cancer cells by multiple mechanisms and may have potential therapeutic use for the prevention and treatment of cancer. (greenmedinfo.com)
  • This study aimed to investigate the effects of xanthoxyletin, a plant-derived coumarin, on human oral squamous cancer cells in vitro and in mouse xenografts in vivo . (medscimonit.com)
  • The study included SCC-1 human oral cancer cells and EBTr normal embryonic bovine tracheal epithelial cells, which were treated with 0 µM, 5 µM, 10 µM, and 20 µM of xanthoxyletin for 24 hours. (medscimonit.com)
  • These effects of honey have been thoroughly investigated in certain cancers such as breast, liver and colorectal cancer cell lines. (mdpi.com)
  • Expression, gene mutation and amplification, methylation, and copy number alteration (CNA) of POGLUT2 were evaluated using The Cancer Genome Atlas (TCGA), Cancer Cell Line Encyclopedia (CCLE), and Genotype-Tissue Expression (GTEx) databases. (frontiersin.org)
  • Increased levels of POGLUT2 were found in multiple types of cancer tissues and cell lines. (frontiersin.org)
  • POGLUT2 was mainly expressed in stromal cells as verified by StromalScore, ESTIMATEScore, ImmuneScore, and Tumor purity, and POGLUT2 was positively correlated with cancer-associated fibroblasts, macrophages, monocytes, and neutrophils in the tumor microenvironment. (frontiersin.org)
  • To investigate the effect of gambogic acid (GA) on apoptosis in the HT-29 human colon cancer cell line. (wjgnet.com)
  • Aiming to exploit this effect in therapy we performed a compound screen in five breast cancer cell lines with IGF neutralising antibody xentuzumab. (nature.com)
  • First, given evidence that IGFs regulate the response to IR, we also found evidence that IGF-1R depletion induced endogenous DNA lesions marked by γH2AX foci in prostate cancer cells [ 10 ]. (nature.com)
  • To investigate the effects of silibinin on the growth of oral cancer cells, cell proliferation and anchorage-independent colony formation tests were conducted on YD10B and Ca9-22 oral cancer cells. (jcancer.org)
  • Western blotting revealed that silibinin downregulated SOD1 and SOD2 and triggered the JNK/c-Jun pathway in oral cancer cells. (jcancer.org)
  • Furthermore, previous studies suggested that knockdown of LAPTM4B , another important subtype of the LAPTM family inhibited proliferation of hepatocellular carcinoma ( 11 ), prostate ( 12 ) and breast cancer cells ( 13 ). (spandidos-publications.com)
  • Here, we explored whether sesamin activates p53, which is widely inhibited in cervical cancer cells, thereby inducing p53-mediated apoptosis. (medsci.org)
  • Human HeLa and SiHa cervical cancer cells and normal Hs68 dermal cells were used as cell models. (medsci.org)
  • Uncontrolled cell proliferation is the hallmark of cancer, and genes that directly regulate the cell cycle are typically damaged in tumor cells ( 10 ). (jcancer.org)
  • Cancer genetics, DNA damage response, apoptosis, cell cycle My current research is focused on two transcription factors that respond to DNA damage and play important roles in regulating tumor development. (mdanderson.org)
  • Apigenin Restrains Colon Cancer Cell Proliferation via Targeted Blocking of Pyruvate Kinase M2-Dependent Glycolysis. (spandidos-publications.com)
  • Our findings suggest that NVD not only directly inhibits the growth of gastric cancer cells but also exerts indirect antitumor effects by enhancing immune function. (hindawi.com)
  • With increasing globalization and advancements in modern medicine, novel therapeutic approaches such as targeted molecular therapy [ 4 ], immunotherapy [ 5 ], and latent/residual tumor cell ablation have been used to improve clinical outcomes for cancer patients, but the prognosis for patients with gastric cancer remains poor. (hindawi.com)
  • Early stage Huh-7 and advanced SNU449 liver cancer cell lines were subjected to chronic alcohol exposure (CAE), at different doses for 6 months followed by 1-month alcohol withdrawal period. (springer.com)
  • CAE induced a dose-dependent increase in cell migration and invasion and also in the expression of cancer stem cells markers (CD133, CD44, CD90). (springer.com)
  • Numerous mechanisms such as acquisition of cancer stem cells (CSCs), a subpopulation characterized by self-renewal and differentiation abilities can explain HCC aggressiveness. (springer.com)
  • Mechanism of miR-455-3 in suppressing epithelial-mesenchymal transition and angiogenesis of non-small cell lung cancer cells. (nih.gov)
  • On the other hand, the infiltration of organs by a monoclonal population of aberrant cells, the possibility of lethal evolution, and the cancer-based modalities of successful treatment are all consistent with a neoplastic process. (medscape.com)
  • These pro- has been implicated as contributing apoptosis, inflammation, and several liferating cel s replace dead cel s to the cancer phenotype, through pleiotropic responses. (who.int)
  • Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)
Invasion3
  • Furthermore, silibinin inhibited the migration and invasion abilities of YD10B and Ca9-22 cells by regulating the expression of proteins involved in the epithelial-mesenchymal transition. (jcancer.org)
  • Analysis of the changes of migration and invasion, showed significant reduced LAPTM5 suppressed cell metastasis. (spandidos-publications.com)
  • CircPAPPA Regulates the Proliferation, Migration, Invasion, Apoptosis, and Cell Cycle of Trophoblast Cells Through the miR-3127-5p/HOXA7 Axis. (nih.gov)
Inflammation2
  • NF-κB pathways are associated with immunity, inflammation and cell survival. (springer.com)
  • Exhibiting high consistence in sequence and structure, S100 family members are interchangeable in function and they show a wide spectrum of biological processes, including proliferation, apoptosis, migration, inflammation and differentiation and the like. (cancerindex.org)
BrdU1
  • Cell proliferation was reduced in a time-dependent manner of drug exposure as revealed from BrdU incorporation and migration studies. (jcimjournal.com)
Pathways6
Vitro3
  • H-29 cells were used for in vitro experiments in this study. (wjgnet.com)
  • In addition to promoting proliferation, SLC25A22 when expressed in vitro, progressed the cell cycle and inhibited apoptosis. (wikipedia.org)
  • To study the possible anticancer and antiproliferative activities of ethanolic leaf extract of Thuja occidentalis (TO) on A549 non-small lung carcinoma cells in vitro . (jcimjournal.com)
Phosphorylation2
  • Endogenous regulation of the cell cycle depends on phosphorylation and dephosphorylation of the cyclin- cyclin-dependent kinase (CDK)-cyclin-dependent kinase inhibitor (CDKI) pathway. (jcancer.org)
  • Two biochemical processes, protein phosphorylation/dephosphorylation and ubiquitin-mediated degradation drive cell cycle control. (benthamscience.com)
METHODS2
Cyclin-depend1
  • The expression of cyclin-dependent kinase inhibitors was profoundly affected with early activation and then repression of p21 cip1/waf1 and persistent activation of both p27 kip1 and p57 kip2 , whereas genes involved in cell survival and proliferation were suppressed. (aacrjournals.org)
Mechanisms3
  • The underlying molecular mechanisms by which androgen-independent PC cells acquire the capacity to proliferate remain largely unclear. (go.jp)
  • Gene expression analysis revealed putative mechanisms of action, which suggested that c-Jun and c-Fos are involved in oleuropein and hydroxytyrosol induced apoptosis of MIA PaCa-2 cells. (uaeu.ac.ae)
  • The purpose of BAY 293 our current research would be to investigate the result of leucine deprivation on breast tumor cells and also to elucidate the root mechanisms. (racetab.org)
Proteins6
  • Subsequent analysis of Bcl-2 family proteins and caspase 3/7 activation determined that oleuropein and hydroxytyrosol induced apoptosis in MIA PaCa-2 cells, while oleuropein displayed a protective effect on HPDE cells. (uaeu.ac.ae)
  • Recombinant proteins for cell culture, standard curves and other applications. (bdbiosciences.com)
  • BD Biosciences has a variety of recombinant proteins that can be used in cell culture and as quantitative standards and controls for various applications. (bdbiosciences.com)
  • During the first stage called G1, cells monitor their environment and, when the requisite signals are received, the cells synthesize RNA and proteins to induce growth. (bdbiosciences.com)
  • Several cell cycle-associated proteins, such as Ki-67, are also used as indicators of cell proliferation. (bdbiosciences.com)
  • Both proteins can localize at the mitochondrion , but the way they control apoptosis still remains unclear. (sdbonline.org)
Genes3
  • For example, the encoded protein represses the transcription of differentiation-specific genes during keratinocyte proliferation, but this repression is then overcome by differentiation signals. (nih.gov)
  • Using differential microarray analysis, we identify target genes that provide the basis for the understanding of the molecular mechanism for the regulation of cell proliferation and transformation mediated by over-expression of the human ChoKalpha. (nih.gov)
  • Only two Bcl-2 family genes have been found in Drosophila melanogaster including the pro-cell survival, human Bok-related orthologue, Buffy. (sdbonline.org)
Vivo1
  • It is immunotoxic in vivo and induces apoptosis in many cultured cells. (cdc.gov)
Transformation2
  • During EMT, cells will undergo transformation from epithelial phenotype to mesenchymal phenotype ( 14 ) and many characteristics of cells will change including loss of cell-cell adhesion and acquisition of aggressive and metastatic ability ( 15 ). (spandidos-publications.com)
  • Choline kinase alpha (ChoKalpha) is an enzyme involved in the metabolism of phospholipids recently found to play a relevant role in the regulation of cell proliferation, oncogenic transformation and human carcinogenesis. (nih.gov)
Mechanism1
Dose-dependent2
  • Silibinin effectively suppressed YD10B and Ca9-22 cell proliferation and colony formation in a dose-dependent manner. (jcancer.org)
  • Sesamin (75 and 150 μM) clearly inhibited SiHa and HeLa cell proliferation in a dose-dependent fashion, but did not affect the proliferation of Hs68 cells. (medsci.org)
Reagents2
  • Monoclonal antibodies as reagents for the analysis of cell surfaces (PhD thesis). (wikipedia.org)
  • BD Biosciences provides a variety of reagents for the identification and quantification of caspase-3 activity in apoptotic cells. (bdbiosciences.com)
Colony formation1
  • Trisomy 8+ cells showed a significant positive correlation with apoptotic CD34+ cells and capacity for colony formation. (who.int)
Colorectal1
  • Silencing of the EBF3 locus was observed in brain, colorectal, breast, liver, and bone tumor cell lines and its reactivation was achieved on treatment with 5-aza-2′-deoxycytidine and trichostatin A in a significant portion of these tumor cells. (aacrjournals.org)
Dendritic cell1
  • The working group of the Histiocyte Society divided histocytic disorders into three groups: (1) dendritic cell histiocytosis, (2) macrophage-related disorders, and (3) malignant histiocytosis. (medscape.com)
Metabolism and cell1
  • The molecular basis to understand the connection between phospholipids metabolism and cell cycle regulation through choline kinase is reported. (nih.gov)
Antigen2
  • Mib-1 monoclonal antibody recognizes the proliferation-related antigen Ki-67. (lu.se)
  • Inflammatory infiltrates in areas positive for VSBV-1 RNA and antigen consisted of CD4+ and CD8+ T cells, with perivascular B-cell accumulation. (cdc.gov)
Immune12
  • While much work has focused on the failure of epithelial cell populations as a key component of the aging process, additional studies have shown that aging, as a global phenomenon in the lung, also impacts resident endothelial, mesenchymal, and immune cell populations. (karger.com)
  • LAPMT5 is a lysosomal membrane protein preferentially expressed in immune cells ( 5 , 6 ) and hematopoietic cells ( 7 ), having a close interaction with the Nedd4 ( 8 ), a member of the E3 ubiquitin ligases family ( 8 ). (spandidos-publications.com)
  • This study is to explore the biomarkers in IDD and to explore the role of immune cell infiltration in IDD. (hindawi.com)
  • Finally, the infiltration of immune cells in the data were analyzed by MCPcounter analysis package. (hindawi.com)
  • In the analysis of immune infiltration, ATF2 was negatively correlated with T cells but positively correlated with neutrophils and endothelial cells. (hindawi.com)
  • The SNHG5/miR-299-5p/ATF2 can be used as biomarker of IDD, and infiltration of immune cells plays an important role in the pathological development of IDD. (hindawi.com)
  • In addition to the above studies, some researchers believe that the occurrence and development of IDD are closely related to immune cell infiltration. (hindawi.com)
  • At the same time, the nucleus pulposus cells release Fas ligand (FasL) tumor necrosis factor to destroy the infiltrating immune cells and maintain immune isolation [ 14 , 15 ]. (hindawi.com)
  • STAT3 protein is crucial in cell proliferation, apoptosis, and host immune responses. (hawaii.edu)
  • In addition to the balance between tumors and the immune system, there is also a balance between tumors and the nonimmune cells that surround them [ 10 ]. (hindawi.com)
  • Thus, both the ability of antitumor drugs to kill tumor cells and their effects on immune function and normal tissue cells should be studied. (hindawi.com)
  • We focus on the characterization of the central nervous system (CNS) immunologic response to VSBV-1 by IHC analyses of immune cells in the brain of all patients, as well as by examination of cerebrospinal fluid (CSF) reactions over time during the disease. (cdc.gov)
Macrophage1
Checkpoint1
Regulation of cell1
  • Moreover, some studies demonstrated that LAPTM5 was highly expressed in malignant B lymphomas and involved in B cell malignancies ( 10 ), involving in negative regulation of cell surface T and B cell receptor by promoting lysosome degradation ( 6 ). (spandidos-publications.com)
Detection5
  • Improve your apoptosis detection skills with our free apoptosis application ebook. (abcam.com)
  • Flow cytometric enumeration of damaged cells through the detection of micronuclei within each cell. (bdbiosciences.com)
  • In addition, you can find purified antibodies for detection of apoptosis using various other techniques. (bdbiosciences.com)
  • BD Biosciences offers BD Horizon™ Violet Proliferation Dye 450 and BD Horizon™ CFSE for the detection of cell proliferation with the violet laser and blue laser, respectively, which facilitates the use of larger panels. (bdbiosciences.com)
  • To support this spectrum of requirements, BD Biosciences offers a full range of apoptosis detection tools and technologies for measuring indicators at different stages across the apoptotic process. (bdbiosciences.com)
Assess cell1
Tumor cell1
  • Open up in another window Shape 2 Leucine deprivation inhibits breasts tumor cell proliferation(A) MDA-MB-231 or CORIN MCF-7 cells had been seeded in 12-well plates and incubated in charge (+leu) or leucine-deficient (?leu) moderate. (racetab.org)
Signaling Pathway1
Kinase3
  • It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. (cancerindex.org)
  • MiR-455-5p upregulation in umbilical cord mesenchymal stem cells attenuates endometrial injury and promotes repair of damaged endometrium via Janus kinase/signal transducer and activator of transcription 3 signaling. (nih.gov)
  • Interestingly, rbf1 -induced apoptosis leads to a debcl - and drp1 -dependent Reactive Oxygen Species production, which in turn activates the Jun Kinase pathway to trigger cell death. (sdbonline.org)
Gene2
  • The expression and significance of the HOXA7 gene in oral squamous cell carcinoma. (nih.gov)
  • Taken all together this demonstrates a role for Buffy, a Bcl-2 pro-cell survival gene, in neuroprotection. (sdbonline.org)
Programmed cell1
  • As cells become damaged or are no longer needed, they undergo apoptosis or programmed cell death, a normal physiological process that occurs during embryonic development and tissue homeostasis. (bdbiosciences.com)
Protein synthesis1
  • Cycloheximide, an inhibitor of protein synthesis, did not alter Cd-induced cytotoxicity indicating that J774A.1 cell death by Cd is independent of de novo protein synthesis including p53. (cdc.gov)
Cytotoxicity3
  • BAY 293 We looked into the cytotoxicity of leucine deprivation on regular cells further, including major hepatocytes, brownish adipose cells (BAT) and MCF-10A cells. (racetab.org)
  • Pretreatment with PD98059 increased Cd-induced cytotoxicity suggesting that ERK activation may be a survival response in the present cell system. (cdc.gov)
  • ERK activation by Cd is not related to decreased proliferation of macrophages but may play a protective role against Cd-induced cytotoxicity. (cdc.gov)
Tissue homeostasis2
  • Stem cells of all organs - including the lung, which harbors distinct stem cells for each separate tissue that makes up the lung as a whole - reside in niches described as a microenvironment that supports and maintains the 'stemness' of cells as a critical reservoir for maintaining tissue homeostasis and responding to injury [ 3 ]. (karger.com)
  • The balance of cell proliferation and apoptosis is important for both development and normal tissue homeostasis. (bdbiosciences.com)
Alteration1
Hallmarks1
  • Immunopathologic examination during infection showed cleavage of caspase 3 in brain cells adjacent to CD8+ cells and widespread p53 expression, hallmarks of apoptosis. (cdc.gov)