• 1 ). By using 3-dimensional aggregates of a highly differentiated intestinal epithelial cell line, the investigators claimed to have established an in vitro cell culture model that "support[s] the natural growth of human noroviruses. (cdc.gov)
  • ECM gel can used with epithelial cells, endothelial cells, muscle cells, nerve cells and tumor cells. (sigmaaldrich.com)
  • In addition, Plk1 also prevented partial epithelial-mesenchymal transition (pEMT) of tubular epithelial cells via autophagy pathway. (nature.com)
  • In this study, we explored the role of Plk1 in the classical kidney fibrosis model, UUO, and cultured fibroblast and renal tubular epithelial cells by using specific Plk1 inhibitors, genetic silencing, and heterozygous global knockout mice. (nature.com)
  • In this study, we found that the SCARA5 expression was lower in CAL-27 and SCC-9 cells than that in human normal oral epithelial keratinocytes. (degruyter.com)
  • In addition, SCARA5 repressed OSCC cell epithelial-mesenchymal transformation (EMT), evidenced by increased E-cadherin expression and reduced N-cadherin expression. (degruyter.com)
  • The primary barriers for invading respiratory pathogens are the airway epithelial cells of the respiratory tract and antimicrobial peptides produced by these cells. (cdc.gov)
  • Significant downregulation of β-defensin1 gene ( DEFB1 ) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)
  • Primary human bronchial epithelial cells HBEp cells. (cdc.gov)
  • Differences in the biological effects of crocidolite asbestos and two glass fibres on epithelial lung cells. (cdc.gov)
  • This study was designed to investigate the effects of substances released from non-injured and injured bovine arterial endothelial cells on 125I-low density lipoprotein uptake and degradation by smooth muscle cells in culture. (lu.se)
  • Injury to endothelial cells by lipid-soluble smoke particles or ultraviolet light, which reduced total cellular protein by 15-25%, enhanced the endothelial cell release of the substances stimulating 125I-low density lipoprotein uptake. (lu.se)
  • article{e328883f-12c1-4d3a-a8e7-8700ca87f7ac, abstract = {{This study was designed to investigate the effects of substances released from non-injured and injured bovine arterial endothelial cells on 125I-low density lipoprotein uptake and degradation by smooth muscle cells in culture. (lu.se)
  • While some islet BM components are produced by endothelial cells (ECs), the source of others remains unknown. (nature.com)
  • We present a rate equation model for the TGF-b pathway in endothelial cells together with novel measurements. (lu.se)
  • endothelial cells. (lu.se)
  • NK cell proliferation, phenotypic expression and cytotoxic activity were evaluated. (springer.com)
  • However, cultures without the presence of MSCs showed higher NK proliferation, expression of activation markers (CD16 and NKG2D) and related cytotoxic activity. (springer.com)
  • The increase in 125I-low density lipoprotein uptake and degradation by smooth muscle cells could be dissociated from cell proliferation. (lu.se)
  • When used in conjunction with FGF and EGF, the maintenance medium will allow for the proliferation of ReNcell immortalized VM and CX neural stem cells. (thomassci.com)
  • In addition to providing biomechanical support to the islets, ECM components are required for β-cell development, proliferation, survival, and proper insulin secretion. (nature.com)
  • SCARA5 overexpression significantly inhibited cell proliferation and induced apoptosis of CAL-27 and SCC-9 cells. (degruyter.com)
  • In conclusion, SCARA5 inhibited the proliferation and EMT and induced the apoptosis of OSCC cells through the inhibition of STAT3 and PI3K/AKT signaling pathways, thereby exerting a tumor suppressor effect. (degruyter.com)
  • The effect of the materials on the cultivated fibroblasts was analyzed in terms of short-term and long-term cell growth as well as in viability and cell proliferation assays. (bvsalud.org)
  • In addition, AKT/mTOR signaling and MAPK signaling were inhibited by JIB extract to suppress melanoma cell growth and proliferation. (medsci.org)
  • The results demonstrated that JIB extract combined with cisplatin enhanced the inhibition of cell growth, proliferation, and survival through the obstruction of cell cycle progression and AKT/mTOR and MAPK signaling as well as the induction of cell apoptosis. (medsci.org)
  • Potential synergistic effects of various combinations including these compounds were tested by measuring cancer cell proliferation and apoptosis. (frontiersin.org)
  • 1994. Mesothelial cell proliferation: A nonspecific response to lung injury associated with fibrosis. (cdc.gov)
  • In modern usage, "tissue culture" generally refers to the growth of cells from a tissue from a multicellular organism in vitro. (wikipedia.org)
  • As a result, cultures are exposed to more physiologically relevant conditions, increasing the predictive value of in vitro experiments. (genengnews.com)
  • Straub TM , Honer zu Bentrup K , Orosz-Coghlan P , Dohnalkova A , Mayer BK , Batholomew RA , In vitro cell culture assay for human noroviruses. (cdc.gov)
  • The specific aim of our project was to develop an in vitro cell culture infectivity assay for human norovirus (hNoV) to enhance risk assessments when these viruses are detected in water supplies. (cdc.gov)
  • Indeed, in vitro cell culture infectivity assays for the waterborne pathogen Cryptosporidium parvum that supplement approved fluorescent microscopy assays do not result in amplification of the environmentally resistant hard-walled oocysts ( 2 ). (cdc.gov)
  • A major challenge with this technique lies in achieving sufficient T cell growth and survival in vitro to obtain a sufficient dose of reactive T cells. (medgadget.com)
  • The present study had aimed to evaluate the effect of a feeder layer of irradiated MSCs in the in vitro expansion of NK cells. (springer.com)
  • Furthermore, we have enriched the hNSCs for the cell surface marker CD133 via magnetic sorting, which has led to an increase in the expansion rate and neuronal fate specification of the hNSCs in vitro. (ca.gov)
  • ReNcell NSC Maintenance Medium (SCM005): ReNcell Neural Stem Cell (NSC) Maintenance Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of ReNcell immortalized human neural progenitor cells. (thomassci.com)
  • In that study investigators failed to observe an increase in viral shedding from symptomatic piglets upon serial passage, despite successful intracellular detection of viral RNA and newly synthesized virus-encoded protein in host cells dying of apoptosis. (cdc.gov)
  • Additionally, JIB extract induced B16/F10 cell apoptosis via the caspase cascade. (medsci.org)
  • The aim of this work was to study its localization and molecular forms in primary cultures, where cell types can be easily distinguished with specific markers. (lu.se)
  • Thus, targeting fibroblast cell activation and pEMT are critical strategies for attenuating fibrosis. (nature.com)
  • Aim: The aim of the present study was to assess the toxicity of different chemically activated GICs in fibroblast cell cultures post-agglutination (PA) and post-setting reaction (PS). (bvsalud.org)
  • The common pathological feature of CKD is the loss of tubular cells and progressive interstitial fibrosis. (nature.com)
  • Some studies showed that blocking cell cycle progression ameliorated fibrosis. (nature.com)
  • Role of stem-like cells in carbon nanotube -induced pulmonary fibrosis. (cdc.gov)
  • Since fibrosis is associated with aberrant tissue repair and extracellular matrix (ECM) accumulation, identifying the cells that are responsible for the repair and ECM production is fundamental to the understanding of fibrosis mechanism. (cdc.gov)
  • We hypothesize that CNTs induce fibroblast stem-like cells (FSCs) and that such induction is essential to the development of fibrosis. (cdc.gov)
  • These cells, isolated from SP-positive FSCs, showed a high expression of type I collagen and alpha-smooth muscle actin, which are key biomarkers of fibrosis, as compared to non-SP cells. (cdc.gov)
  • The synthesized tumor cells allow scientists to diagnose cancer and optimize treatment without putting the patient through tiresome rigors of chemotherapy trial and error. (eurekalert.org)
  • They captured diseased cells voided in the dogs' urine and replicated them using a new process that generates bodies mimicing key characteristics of the original tumor cells closely enough that they can effectively be used to diagnose disease and identify possible treatments. (eurekalert.org)
  • It is suggested that curcumin can selectively kill tumor cells through its multifaceted metabolic effects, that culminate in its anti-oxidant and anti-inflammatory activities ( Hewlings and Kalman, 2017 ). (frontiersin.org)
  • Madin Darby Canine Kidney (MDCK) cells were cultured in Eagle's Minimum Essential Medium (ATCC, Manassas, VA) with 10% fetal bovine serum, and appropriate antibiotics. (cdc.gov)
  • GMP-Compatible, Xeno-Free Culture of Human Induced Mesenchymal Stem Cells. (bvsalud.org)
  • Mesenchymal stem cells (MSCs) have been used in therapies owing to their regenerative potential, paracrine regulatory effects, and immunomodulatory activity. (bvsalud.org)
  • With over 20-year professional experience in cell line and cell culture development, and achieved world record high mammalian cell line productivity, he is an international recognized scientist / expert and frequently invited keynote speaker / chairperson / scientific advisor for international conferences, and also a member of Editor and Advisor Board for Bioprocess International Journal, and reviewer for several scientific and professional Journals and authored numerous publications and presentations. (selectbiosciences.com)
  • Interactions between light and various cells in cultures, such as bacteria or mammalian cells, are widely applied for optical sensors and optofluidic systems. (mdpi.com)
  • However, while many studies demonstrated the importance of the ECM, its interactions with β-cells are still not fully understood. (nature.com)
  • The interactions with the various BM components differentially affect β-cells, although the underlying mechanism is largely unknown. (nature.com)
  • Here we compared two microfluidic devices for the recovery of circulating melanoma cells. (nature.com)
  • Melanoma tumour cells exhibit pronounced heterogeneity within a single tumour, amongst concurrent tumours within the same patient and amongst tumours from different patients. (nature.com)
  • The heterogeneous expression of markers, together with their rare frequency (1-10 CTCs in 8 mL of blood) are the main intrinsic factors hampering CTC isolation and limiting in-depth study of these cells in patients with melanoma. (nature.com)
  • In this study, we compared the recovery and purity of melanoma cells isolated through different microfluidic devices, Parsortix (Angle plc, Surrey, United Kingdom) and ClearCell® FX1 system (Biolidics, Singapore), which enrich CTCs based on their differential cell size, density and deformability. (nature.com)
  • The melanoma cell lines A2058 and SK-MEL-5 were used in spiking experiments due to their differential cell size. (nature.com)
  • extract (JIB extract) for melanoma cells. (medsci.org)
  • Collectively, our results indicate that JIB extract showed anti-tumor effects and synergized with cisplatin against B16/F10 cells, indicating the possibility of JIB extract to be developed as adjuvant therapy for melanoma. (medsci.org)
  • Researchers at the Harvard Wyss Institute have developed a biomaterial scaffold that mimics the actions of antigen-presenting cells (APCs) in stimulating T cell growth and survival. (medgadget.com)
  • NK expansion and activation were stimulated by culture with artificial antigen-presenting cells (aAPCs) and IL-2, in the presence or absence of BM-MSCs. (springer.com)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. (wikipedia.org)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • The scaffold allowed the researchers to significantly expand T cell numbers in a dish, compared with existing culture methods, and could bring T cell therapies, such as anti-cancer treatments, closer to clinical reality. (medgadget.com)
  • Common methods for the generation of human embryonic-derived neural stem cells (hNSCs) result in cells with potentially compromised safety profiles due to maintenance of cells in conditions containing non-human proteins (e.g. in bovine serum or on mouse fibroblast feeders). (ca.gov)
  • Double-staining studies with specific cell markers to distinguish astrocytes, neurons and oligodendrocytes were performed. (lu.se)
  • COMT immunoreactivity colocalized with a specific oligodendrocyte marker galactocerebroside in cells displaying oligodendrocyte morphology, flat cells displaying type-1 astrocyte morphology and glial fibrillary acidic protein, in branched cells displaying type-2 astrocyte morphology and in cell bodies of neurons, the processes of which displayed neurofilament immunoreactivity. (lu.se)
  • The results suggest that COMT is synthesized by cultured astrocytes, oligodendrocytes and neurons. (lu.se)
  • ReNcell CX Immortalized Cells (SCC007): ReNcell CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. (thomassci.com)
  • Their many advantages include a long-range cell-targeting action, low toxicity, low immunogenicity, high stability and the capacity to encapsulate proteins, drugs or nucleic acids. (anton-paar.com)
  • Western blots detected both soluble 24 kDa and membrane-bound 28-kDa COMT proteins in neuronal and astrocyte cultures. (lu.se)
  • Targeted therapy drugs are used to target specific genes and proteins of cancer cells to precisely identify and attack specific types of cancer cells. (medsci.org)
  • But in 2018, researchers in Lund started using different human proteins needed for cell survival in a cell culture medium. (lu.se)
  • CD133-enriched Xeno-Free human embryonic-derived neural stem cells expand rapidly in culture and do not form teratomas in immunodeficient mice. (ca.gov)
  • Here, we report a strategy that produces hNSCs in completely 'Xeno-Free' culture conditions. (ca.gov)
  • For therapeutic applications, human iMSCs must be produced in xeno-free culture conditions and following procedures that are compatible with the principles of Good Manufacturing Practice. (bvsalud.org)
  • The human embryo is a source of vital cells used in regenerative medicine, as well as a powerful symbol of life. (lu.se)
  • In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph. (wikipedia.org)
  • He holds both M.S. and Ph.D. degrees in molecular cell biology and biotechnology from Illinois Institute of Technology. (selectbiosciences.com)
  • International Journal of Biochemistry & Cell Biology, 40 , 2335-2340. (springer.com)
  • Natural killer cell biology: An update and future directions. (springer.com)
  • As members of the Department of Cell Biology we are all greatly privileged to be able to pursue our dreams of discovering new knowledge, widening horizons, and improving the well-being of future generations, both in our own communities and throughout the world. (duke.edu)
  • Given this background, it is the goal of the Department of Cell Biology to foster a supportive and inclusive work environment in which the guiding principles of scientific integrity, innovation, open communication, training, career advancement and discovery research can flourish. (duke.edu)
  • It is the responsibility of every PI or group leader to make sure that all members of their lab, group, Initiative or Center associated with Cell Biology are aware of the Plan, and they should review and discuss it with new members as soon as possible. (duke.edu)
  • One presentation at the summit underscored the potential of human pluripotent stem-cell-derived organoids to help address viral diseases such as COVID-19 through disease modeling and drug screening. (corning.com)
  • Human pluripotent stem cells, with their ability to proliferate indefinitely and to differentiate into virtually all cell types of the human body, provide a novel resource to study human development and to implement relevant disease models. (mdpi.com)
  • Here, we employed a human pancreatic differentiation platform complemented with an shRNA screen in human pluripotent stem cells (PSCs) to identify potential drivers of early endoderm and pancreatic development. (mdpi.com)
  • We have established a novel type of pluripotent neural crest-like stem cells (NCLSCs) from neonatal mouse epidermis (Sviderskaya et al. (selectbiosciences.com)
  • When doing studies with human samples, including iPS (induced pluripotent stem) cells or embryonic stem cells, be particularly careful about possible restrictions on their use and terms of IRB (Institutional Review Board) approvals. (duke.edu)
  • To foster commercialization and implementation of stem cells treatments , researchers have recently derived MSCs from human induced pluripotent stem cells (iMSCs). (bvsalud.org)
  • The lifespan of most cells is genetically determined, but some cell-culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (wikipedia.org)
  • Professor Usui's team set out to create a type of organoids without the use of cell-stimulating supplements and Matrigel, a costly product derived from stem cells harvested from genetically engineered rodents. (eurekalert.org)
  • Elena has a long-standing interest in the genetic and physiological regulation of mammalian pigmentation, with a special focus on determination of neural crest stem cells. (selectbiosciences.com)
  • 2018). We further discovered that metabolic reprograming in neural stem cells irreversibly converts them into tumor stem cells initiating malignant overgrowth (Bonnay et al. (europa.eu)
  • ReNcell CX Human Neural Progenitor Cell Line & Culture Media Kit ReNcell CX Kit contains human neural progenitor cells, optimized maintenance media & freezing media for culture of human neural stem cells. (thomassci.com)
  • Withdrawal of the growth factors from ReNcell NSC Maintenance Medium will result in the spontaneous differentiation of ReNcell immortalized neural progenitor cells. (thomassci.com)
  • ReNcell NSC Freezing Medium (SCM007): ReNcell NSC Freezing Medium is qualified for use with ReNcell immortalized human neural progenitor cell lines, CX (CHEMICON Catalog No. SCC007) and VM (CHEMICON Catalog No. SCC008) cultured in serum-free conditions with ReNcell NSC Maintenance Medium (CHEMICON Catalog No. SCM005). (thomassci.com)
  • We may not have been able to gather together in person this year, but the 2021 Virtual 3D Cell Culture Summit was no less of a smash. (corning.com)
  • While in infected A549 and Vero cells a pronounced cytopathic effect was observed COS7, 293T and Huh7.5 cells were most resistant. (wjgnet.com)
  • Title : Japanese Encephalitis (JE) vaccine = GRADE for inactivated Vero cell culture- derived JE vaccine (JE-VC) Personal Author(s) : Walter, Emmanuel B.;Hills, Susan L. (cdc.gov)
  • Triple treatment showed synergism in terms of exhibiting anti-tumorigenic effects by activating the Hippo YAP signaling pathway in the HCT116 cell line. (frontiersin.org)
  • In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered by cells overexpressing DEFB1 , suggesting that this may be one of the pathways by which defensin regulates IAV replication in HBEpCs. (cdc.gov)
  • image: CK5 expression, a basal cell marker, in the 2.5D organoid cells (right) and parental 3D organoids (left). (eurekalert.org)
  • The team created the organoids by culturing the captured diseased cells in a protein-rich medium. (eurekalert.org)
  • Once the team succeeded in creating organoids from the dogs' diseased cells, they tested their response to three common anti-cancer drugs as well as several antibodies. (eurekalert.org)
  • The cells became tumors as anticipated, giving the team further evidence that 2.5D organoids may be an accurate and effective research medium. (eurekalert.org)
  • Industry leaders exchanged ideas about getting started in 3D, workflow optimizations, and the future of 3D cell culture applications, from spheroids and organoids to tissue models. (corning.com)
  • After developing a panel of hPSC-derived cells and organoids and screening ten different types, Dr. Chen and team found that lung, colon, heart, liver, and pancreatic cells could be infected by the novel coronavirus. (corning.com)
  • We use a tissue culture system called cerebral organoids that we have developed in 2013 and that can recapitulate brain development at a remarkable level of detail (Lancaster et al. (europa.eu)
  • The cells are bathed in a culture medium, which contains essential nutrients and energy sources necessary for the cells' survival. (wikipedia.org)
  • The researchers designed a biomaterial scaffold that provides pro-survival and pro-growth biological cues to T cells, just like an APC in the body. (medgadget.com)
  • They loaded the rods with Interleukin-2, a protein produced by APCs that can enhance T cell survival, and coated them using a lipid layer, to mimic the APC cell membrane. (medgadget.com)
  • Our approach closely mimics how APCs present their stimulating cues to primary T cells on their outer membrane and how they release soluble factors that enhance the survival of the T cells," said David Mooney, a researcher who led the study. (medgadget.com)
  • In conclusion, the reduction in IAV copy number in DEFB1 overexpressing cells suggests that β-defensin-1 plays a key role in regulating IAV survival through STAT3 and is a potential target for antiviral drug development. (cdc.gov)
  • This suggests that the demonstration of cytopathic effect and virus internalization in cells alone may not provide direct evidence of productive virus replication. (cdc.gov)
  • 2014). Human NK cells: From surface receptors to the therapy of leukemias and solid tumors. (springer.com)
  • Procko examined more than 2,000 ACE2 mutations and created cells with the mutant receptors on their surfaces. (scitechdaily.com)
  • They are preferentially expressed by a variety of adenocarcinomas, including breast, prostate, ovarian and pancreatic carcinomas as well as malignant plasma cells of multiple myelomas. (sigmaaldrich.com)
  • Pancreatic pericytes directly support β-cells through mostly unidentified secreted factors. (nature.com)
  • Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
  • The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. (wikipedia.org)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). (wikipedia.org)
  • In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
  • Ross Granville Harrison, working at Johns Hopkins Medical School and then at Yale University, published results of his experiments from 1907 to 1910, establishing the methodology of tissue culture. (wikipedia.org)
  • Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture. (wikipedia.org)
  • He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • C trachomatis grows well in a variety of cell lines (eg, McCoy and HeLa cells) that can be maintained in tissue culture. (medscape.com)
  • Incubation in tissue culture is 40-72 hours, depending on the cell type and specific biovar. (medscape.com)
  • Over four weeks of culturing, the differentiated yet integrated tissues maintained their molecular, structural, and functional phenotypes, indicating their potential durability and reliability in immune-oncology applications. (corning.com)
  • Understanding of molecular mechanisms in natural killer cell therapy. (springer.com)
  • It was demonstrated that endothelial cell-released non-dialysable (molecular weight cut off 12-14000) substances significantly stimulated 125I-low density lipoprotein uptake and degradation by smooth muscle cells. (lu.se)
  • Exosomes are nanometer-range vesicles originating from the fusion of endosomes with the plasma membrane of eukaryotic cells. (anton-paar.com)
  • Due to its key role in the eukaryotic cell cycles, Plk1 has been one of the most validated drug targets for cancer treatment. (nature.com)
  • Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology. (wikipedia.org)
  • In contrast, culturing on Laminin-421 induced the expression of β-cell genes, including Ins1 , MafA , and Glut2 , and significantly improved glucose-stimulated insulin secretion. (nature.com)
  • Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture. (wikipedia.org)
  • Adherent cell culturing is a space intensive task, running the risk of scores of traditional flasks sprawling across lab benches or filling incubators to reach required surface areas. (corning.com)
  • Grow large quantities of adherent cells in a compact space with Corning's HYPER technology. (corning.com)
  • The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
  • This vaccine was made possible by the cell culture research of John Franklin Enders, Thomas Huckle Weller, and Frederick Chapman Robbins, who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • Cell culturing is integral to diverse research fields including cell therapy, gene therapy, diagnostics, and vaccine development, among others. (corning.com)
  • Researchers in Japan have replicated cancer cells from diseased bladder tissue in dogs, minimizing the use of costly stem cell products. (eurekalert.org)
  • To study this new cell culture approach, the researchers focused on bladder cancer in dogs, a disease which occurs at a rate similar to humans. (eurekalert.org)
  • Researchers at the Harvard Wyss Institute took inspiration from another immune cell type, the APC, which stimulates T cells to grow and survive during an immune response. (medgadget.com)
  • Using the scaffolds, the researchers were able to expand T cells from mice and humans much faster than existing culture techniques, and they confirmed that the expanded cells have clinical potential in a mouse lymphoma model. (medgadget.com)
  • In the culture vessel comparison, the researchers used a "traditional" large-capacity (6,320 cm 2 surface area)10-layer vessel and Corning HYPER technology allowing for a more compact (1,720 cm 2 ) 10-layer vessel. (corning.com)
  • Researchers from Lund have developed a completely animal-free preparation for cell culture, a medium that can be used to grow human cells, including for cancer research. (lu.se)
  • It took off in 1958 when researchers discovered that fetal bovine serum (FBS) was an additive that could make cells thrive, both normal and cancerous. (lu.se)
  • Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
  • While the authors provide compelling evidence of successful virus infection through microscopy, hybridization of viral RNA after 5 passages in cell culture, and preliminary evidence of viral RNA replication through limiting dilution PCR, we question the level of virus replication that is actually achieved in this system. (cdc.gov)
  • As further validation of our published findings, we have now optimized RT quantitative PCR to assess the level of viral production in cell culture, where we are finding increases in viral titer. (cdc.gov)
  • When biologists from the Cer Groupe immunobiology laboratory in Belgium wanted to improve viral production yields for use in diagnostic kits, they ran a performance comparison for stacked (multi-layer) culture vessels. (corning.com)
  • Cells were analyzed 48 h post infection for the amount of intracellular and extracellular viral genomes and infectious viral particles by quantitative real-time PCR and virus titration assay. (wjgnet.com)
  • Although the analyzed cell lines released comparable amounts of viral genomes to the supernatant significant differences were found for the number of infectious viral particles. (wjgnet.com)
  • Oral squamous cell carcinoma (OSCC) is a common tumor in the world. (degruyter.com)
  • Besides, 12% of patients have second- or third-degree skin reactions that are sensitive to light, and about 18% of patients develop cutaneous squamous-cell carcinoma and keratoacanthoma or occurred at the same time [ 6 ]. (medsci.org)
  • Monoclonal Anti-MUC1 (mouse IgG1 isotype) is derived from the hybridoma 1D1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with semi-purified MUC1 from pleural effusion of a breast cancer patient. (sigmaaldrich.com)
  • Cell culture biotech startup Avant has launched a multifunctional protein for the active beauty market made from cultivated fish cells - an ingredient it says will carve out an entirely new segment in anti-ageing and protective skin care. (cosmeticsdesign-europe.com)
  • We investigated the effects of different level of HA concentrations on mRNA and protein expressions of MMP-2 and MMP-9 in cultured trabecular meshwork cells using reverse transcription-polymerase chain reaction (RT-PCR) method and gelatin zymography analysis. (molvis.org)
  • To infect a human cell, a virus must first bind to a receptor protein on the surface of the cell. (scitechdaily.com)
  • JIB extract induced cell cycle arrest at the G 0 /G 1 phase and decreased cyclin and cdk protein expressions. (medsci.org)
  • Protein collected from cells infected or uninfected using RIPA lysis buffer. (cdc.gov)
  • Scientists also added various protein extracts from animals to make the cells grow. (lu.se)
  • Trabecular meshwork cells were cultured from trabecular tissues obtained from the POAG patients aged 23 to 45. (molvis.org)
  • Immortalized by retroviral transduction with the c-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. (thomassci.com)
  • We investigated the effect of HA on the expression of matrix metalloproteinases (MMP-2 and MMP-9) in cultured trabecular meshwork cells. (molvis.org)
  • Whereas α2 laminins promoted islet cell clustering, they did not affect gene expression. (nature.com)
  • Fluorescence activated cell sorting was used to isolate FSCs from CNT-treated normal human lung fibroblasts (NHLFs).The expression of stem cell markers and fibrogenic markers was examined using western blotting, immunofluorescence staining and confocal microscopy. (cdc.gov)
  • Our results demonstrated for the first time that CNTs can induce FSCs from NHLFs as evidenced by their side population (SP) property and expression of stem cell markers ABCG2 and ALDH1A1. (cdc.gov)
  • These cells may be cells isolated from a donor organism (primary cells) or an immortalised cell line. (wikipedia.org)
  • COMT immunoreactivity was studied in primary astrocytic cultures from newborn rat cerebral cortex, and in neuronal cultures from rat brain from 18-day-old rat embryos using antisera against rat recombinant COMT made in guinea pig. (lu.se)
  • The Quasi Vivo ® System consists of interconnected cell culture chambers and a peristaltic pump to create a continuous flow of media over cells. (genengnews.com)
  • The Quasi Vivo System is available with three different culture chambers (QV500, QV600, and QV900) to support a wide range of applications, including submerged cell culture, co-culture, and modeling of air-liquid and liquid-liquid interfaces. (genengnews.com)
  • Shaping of natural killer cell antitumor activity by ex vivo cultivation. (springer.com)
  • Hence, recapitulating the islet ECM in both isolated islets and stem-cell-derived β-cells is of high therapeutic value. (nature.com)
  • In this study, we investigated a plausible therapeutic synergism of a triple combination of CBD/CBG, curcumin, and piperine in the colon adenocarcinoma using HCT116 and HT29 cell lines. (frontiersin.org)
  • Dr. Jianguo Yang is principal scientist leading projects on cell line / cell culture development at Genzyme/ Sanofi. (selectbiosciences.com)
  • This presentation is an overview for the major challenges, including vector and host engineering, clone selection and cell line stability, cell banking, platform medium and process development, product quality assessment and their future trends. (selectbiosciences.com)
  • Although, non-psychoactive cannabidiol (CBD) and cannabigerol (CBG) display antineoplastic effects by repressing tumor growth and angiogenesis both in cell line and animal models, their use as chemotherapeutic agents is awaiting further investigation. (frontiersin.org)
  • Cytology is used mainly for diagnosing infant inclusion conjunctivitis and ocular trachoma through the demonstration of intracytoplasmic C trachomatis inclusions in HeLa cells (ie, continuously cultured carcinoma cell line used for tissue cultures). (medscape.com)
  • Ideally cell line sensitivity should be known for all frozen stocks and evaluated whenever fresh cells are resuscitated or received in the laboratory. (who.int)
  • This facility allows for walk-in usage by users and will soon provide services including cell passage, storage, transfection, etc. (umass.edu)
  • We are confident that our method will make a breakthrough in bladder cancer diagnosis and treatment in both animals and humans, as it efficiently reduces the culture time, handling, gel, and media supplement costs, and therefore it can be an important platform for the development of new bladder cancer therapy," Usui said. (eurekalert.org)
  • 2016). Natural killer cell-based adoptive immunotherapy eradicates and drives differentiation of chemoresistant bladder cancer stem-like cells. (springer.com)
  • Cultivate most cell types without matrix coating when using Thermo Scientific™ Nunc™ Polycarbonate Membrane Inserts in Multidishes. (thermofisher.com)
  • β-Cells depend on the islet basement membrane (BM). (nature.com)
  • The peri-islet membrane, which encapsulates the islets, separates the endocrine cells from the exocrine pancreas and serves as a barrier from immune cell infiltration to the islets. (nature.com)
  • Thus, human endocrine cells are not in direct contact with the vascular BM components but with the invaginated peri-islet membrane. (nature.com)
  • They can be isolated from cell culture medium as well as from many extracellular fluids such as blood, urine, saliva, breast milk and cerebrospinal fluid. (anton-paar.com)
  • In this report, exosomes isolated from the cell culture medium of human buccal cells were characterized using a Litesizer™ 500. (anton-paar.com)
  • We have developed a completely animal product-free cell medium and it worked immediately - we could repeat with great precision. (lu.se)
  • Adding FBS to a cell culture medium is like working with a black hole. (lu.se)
  • We show that it is possible to grow cells successfully with a completely defined and animal product-free medium," says Stina Oredsson. (lu.se)
  • Featuring 26 speakers who logged in from around the world, the event captured the energy and excitement of the rapidly evolving 3D cell culture movement. (corning.com)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • 2009, FASEB J). These cells were isolated as three independent immortal lines. (selectbiosciences.com)
  • Various human and non-human cell lines were infected with a defined amount of ZIKV Polynesia strain. (wjgnet.com)
  • All investigated cell lines except CHO cells supported infection, replication and release of ZIKV. (wjgnet.com)
  • When using cell lines or mouse strains, have accurate records of their origin and genetic background, and how they are identified and maintained. (duke.edu)
  • Mali showed that p-STAT3 was positively expressed in OSCC cell lines [ 15 ]. (degruyter.com)
  • Our findings revealed that different genetic backgrounds of HCT116 and HT29 cell lines resulted in divergent responses to the combination treatments. (frontiersin.org)
  • Figure] out how complex you need it, and then involve patient-specific predictions using patient cells to do drug screening and disease modeling to try to find the best drug for you. (corning.com)
  • Anti-cancer T cell therapies involve culturing and sometimes modifying patient-specific T cells in a dish before administering them to a cancer patient, where they can attack and kill cancer cells. (medgadget.com)
  • Because of its high specificity (100%) and sensitivity, cell culture is the only test that should be used to establish the presence or absence of infections in cases with legal implications, such as those involving rape or sexual abuse. (medscape.com)
  • Using foetal cells from aborted embryos for transplantation to the brains of Parkinson patients is an avenue that has been explored by neuroscientists on and off for the last thirty years. (lu.se)
  • While each tissue is cultured in its own optimized environment, the whole is integrated through a recirculating vascular flow with monocytes by a matured and selectively permeable endothelial barrier. (corning.com)