Cell culture 2018. Medical search. Frequent questions

In recent years, with increasing interest being directed toward cultured cell-based production of vaccines, and in vivo proteins such as enzymes, hormones, antibodies and cytokines, as well as cell grafts for use in regenerative medicine, greater focus has been placed on efficient and convenient methodologies for mass cell culturing. (justia.com)
Under this agreement, FDA will oversee the collection, growth, and the differentiation of living cells into various cell types, such as proteins and fats. (usda.gov)
IFN-α 2A enhances the expression of MHC proteins by increasing the presentation of antigen peptides and activates cytotoxic T cells. (sigmaaldrich.com)
hPSC-enriched essential genes mainly encode transcription factors and proteins related to cell-cycle and DNA-repair, revealing that a quarter of the nuclear factors are essential for normal growth. (nature.com)
But in 2018, researchers in Lund started using different human proteins needed for cell survival in a cell culture medium. (lu.se)

A 2021 study suggests that "cell-based seafood" outperformed "cell-cultured seafood" based on the perceptions of 1,200 adult participants. (healthline.com)
2018-2021 obtained my Master's degree (M.Sc. (nki.nl)

Another important component for the devices is the ability to produce stable gradients that are present in vivo as these gradients play a significant role in understanding chemotactic, durotactic, and haptotactic effects on cells. (wikipedia.org)
Spatial organization of cells in microscale devices largely depends on the culture region geometry for cells to perform functions in vivo. (wikipedia.org)
Laminar flow is also useful as is it mimics in vivo fluid dynamics more accurately, often making microscale culture more relevant than traditional culture methods. (wikipedia.org)
The Quasi Vivo ® System consists of interconnected cell culture chambers and a peristaltic pump to create a continuous flow of media over cells. (genengnews.com)
The Quasi Vivo System is available with three different culture chambers (QV500, QV600, and QV900) to support a wide range of applications, including submerged cell culture, co-culture, and modeling of air-liquid and liquid-liquid interfaces. (genengnews.com)
3-D cell culture recapitulates in vivo environments for generating physiologically relevant extracellular vesicles. (the-scientist.com)
However, important and distinct differences exist between 2D and 3D cell culturing, as well as the in vivo situation. (norecopa.no)
To understand the fundamental connections between particle properties and human and environmental toxicology we study toxicological effects on all levels, from single cells to ecosystems using various in-vitro, ex-vivo, and in-vivo methods. (lu.se)

Using human pluripotent stem cells, scientists engineered an amniotic sac-like structure, potentially useful for uncovering the elusive cellular signals that lead to infertility. (the-scientist.com)
Using our recently established haploid human pluripotent stem cells (hPSCs), we generated a genome-wide loss-of-function library targeting 18,166 protein-coding genes to define the essential genes in hPSCs. (nature.com)
Genotoxic Effects of Culture Media on Human Pluripotent Stem Cells. (ncbs.res.in)
Sozzi E, Nilsson F, Kajtez J, Parmar M, Fiorenzano A. Generation of Human Ventral Midbrain Organoids Derived from Pluripotent Stem Cells . (lu.se)

Perfusion in microfluidic cell culture is important to enable long culture periods on-chip and cell differentiation. (wikipedia.org)
Effect of fiber diameter on spreading, proliferation, and differentiation of osteoblastic cells on electrospun poly (lactic acid) substrates. (springer.com)
The influence of fiber diameter of electrospun substrates on neural stem cell differentiation and proliferation. (springer.com)
Derivation and differentiation of haploid human embryonic stem cells. (nature.com)
In this framework, the FDA would oversee cell collection, cell banks, cell growth and cell differentiation. (thompsoncoburn.com)
PKC is an important signaling molecule in numerous cell pathways, including cell differentiation, proliferation and apoptosis. (medscape.com)
Photobiomodulation therapy improves multilineage differentiation of dental pulp stem cells in three-dimensional culture model. (bvsalud.org)
Photobiomodulation therapy (PBM) has shown positive effects on stem cell differentiation in monolayer cell culture model, but little is known about its effect on three-dimensional (3-D) agarose gel culture . (bvsalud.org)
This study evaluated the PBM effect of human dental pulp stem cells (hDPSCs) differentiation and phosphatase alkaline activity (ALP) using an agarose 3-D model under different nutritional conditions. (bvsalud.org)

Consumption of media substrates and production of waste by the cells create a dynamic osmolality profile. (labmanager.com)

On March 7, 2019, FDA and FSIS agreed to establish a joint regulatory framework for human foods made from cultured cells of livestock and poultry to help ensure that any such products brought to market are safe, unadulterated, and truthfully labeled. (usda.gov)
I would like to welcome you to today's COCA Call: 2018-2019 Influenza Season and Recommendations for Clinicians. (cdc.gov)

Inactivated Vero cell culture-derived JE vaccine (Ixiaro [JE-VC]) is the only JE vaccine that is licensed and available in the United States. (cdc.gov)
Therefore, the aim of this research was to evaluate the platelet-rich plasma (PRP) as a supplement and scaffold for Vero cell culture, in a one case report study. (springer.com)
The morphological analysis, carried out on the third and fifth days, did not verify changes in the typical morphology of Vero cells cultured with SPRP. (springer.com)
Therefore, in Vero cell cultures, the results suggest that SPRP and CPRP can be used as a supplement and scaffold, respectively. (springer.com)
While in infected A549 and Vero cells a pronounced cytopathic effect was observed COS7, 293T and Huh7.5 cells were most resistant. (wjgnet.com)
Title : Japanese Encephalitis (JE) vaccine = GRADE for inactivated Vero cell culture- derived JE vaccine (JE-VC) Personal Author(s) : Walter, Emmanuel B.;Hills, Susan L. (cdc.gov)
Infectious virus was detected by Vero cell culture and plaque assay. (medscape.com)

Cells generally exist as three-dimensional aggregates in the body, but in classical plate culturing, cells are cultured in a monolayer fashion with the cells attached to a vessel. (justia.com)
Both 2D monolayer and 3D neurospheres were successfully cultured and characterized for proliferative potential, intrinsic plasticity, presence of cancer stem cells and the expression of stem cell markers. (frontiersin.org)

On November 16, 2018, the USDA and FDA announced their intention to "jointly oversee the production of cell-cultured food products derived from livestock and poultry. (thompsoncoburn.com)

Towards integrated production of an influenza A vaccine candidate with MDCK suspension cells. (mpg.de)
Perfusion Control for High Cell Density Cultivation and Viral Vaccine Production. (mpg.de)
Efficient influenza vaccine manufacturing: Single MDCK suspension cells in chemically defined medium. (mpg.de)
This stage of the process is conducted if suspected polioviruses are identified in the cell culture under a microscope, in order to find out what kind of virus it is - vaccine virus, vaccine-derived poliovirus or wild poliovirus. (who.int)

Also, in order to isolate other viruses of interest, PIM cultured in some common cell lines ( see Survey Guidance Document: Appendix C: Common Cell Lines and Animals Susceptible to Poliovirus* ) may have amplified poliovirus unintentionally. (cdc.gov)

For example, long, narrow channels may be desired to culture neurons. (wikipedia.org)
Compartmentalized microfluidic cultures have also been combined with live cell calcium imaging, where depolarizing stimuli have been delivered to the peripheral terminals of neurons, and calcium responses recorded in the cell body. (wikipedia.org)

Epub 2018 Apr 28. (nih.gov)
Epub 2018 Oct 12. (nih.gov)
Epub 2018 Nov 8. (nih.gov)
Epub 2018 Jul 26. (nih.gov)
Epub 2018 Feb 2. (nih.gov)
Epub 2018 Feb 22. (nih.gov)

Microfluidic cell culture integrates knowledge from biology, biochemistry, engineering, and physics to develop devices and techniques for culturing, maintaining, analyzing, and experimenting with cells at the microscale. (wikipedia.org)
Microfluidics has been used for cell biology studies as the dimensions of the microfluidic channels are well suited for the physical scale of cells (in the order of magnitude of 10 micrometers). (wikipedia.org)
There is a long history of scientific advances in biology, biochemistry, and engineering that have led to the innovations enabling the growth of animal cells outside of the animal itself, in a controlled environment, for food. (usda.gov)
2018. Discovery biology of neuropsychiatric syndromes (DBNS): a center for integrating clinical medicine and basic science. . (ncbs.res.in)

Scientists typically start with a sample of cells from the tissue of an animal or fish, a process that typically does not permanently harm or kill the animal. (usda.gov)
After the cells have multiplied many times over into billions or trillions of cells, additional factors (e.g., protein growth factors, new surfaces for cell attachment, additional nutrients) are added to the controlled environment to enable the cells to differentiate into various cell types and assume characteristics of muscle, fat, or connective tissue cells. (usda.gov)
Tissue engineering performs the culture of cells on scaffolds, aiming at the restoration of damaged tissue. (springer.com)
Tumor tissue from a rare non-infantile 8-year-old female DIG patient was dissociated and digested using collagenase to establish live cultures. (frontiersin.org)
Cells cultured as 3D were embedded as tissue blocks. (frontiersin.org)
Using immunohistochemistry, the 3D cultures were shown to express markers as in the original DIG tumor tissue indicating that the spheroid cultures were able to maintain the heterogeneity found in the original tumor. (frontiersin.org)
The tumor is heterogeneous with meningeal tumor cells, mainly a mixture of fibroblast like cells, and neuroepithelial cells in a background of connective tissue. (frontiersin.org)
Waweru D, KuriaE, BradleyJ, Scholes J, Runo S. Tissue Culture Protocols for the Obligate Parasitic Plant Striga hermonthica and Implications for Host-Parasite Co-cultivation. (ac.ke)
It can be concluded that hDPSCs cultured in 3-D agarose associated to PBM could be a promising tool for tissue engineering applications. (bvsalud.org)

Chrysin regulated miR-18a, miR-21, and miR-221 genes in gastric cancer cell line [ 26 ]. (hindawi.com)
Fig. 2: Analysis of cell-essential genes. (nature.com)

In his search for effective therapies for Parkinson's disease, Lorenz Studer is uncovering pluripotency switches and clues to what makes cells age. (the-scientist.com)

Leeb, M. & Wutz, A. Derivation of haploid embryonic stem cells from mouse embryos. (nature.com)
Forward and reverse genetics through derivation of haploid mouse embryonic stem cells. (nature.com)
2018. Derivation of iPSC lines from two patients with familial Alzheimer's disease from India. . (ncbs.res.in)

The evaluation of SPRP as a supplement showed that there was no statistical difference in cell viability compared to cultures supplemented in the standard way, with fetal bovine serum (FBS), after 24 h of culture. (springer.com)
It took off in 1958 when researchers discovered that fetal bovine serum (FBS) was an additive that could make cells thrive, both normal and cancerous. (lu.se)

HumanKine IFN-α 2A, expressed in human HEK 293 cells, is a glycosylated monomer with an apparent molecular mass of 16 kDa. (sigmaaldrich.com)

Established in 2011, Berkeley Lights Inc. (BLI) is an American corporation that provides products and services for biomedicines, genome analysis and cell treatment using its unique OptoSelect *1 technology. (nikon.com)

As a solution for the issue of oxygen supply, it has been attempted to increase the oxygen concentration by utilizing microbubbles (PTL 1) or to employ methods for uniformly supplying oxygen in microcarrier culturing (PTL 2). (justia.com)
Methods of culturing while exposing the cell culture support to a gas phase have also been attempted. (justia.com)
These reduced cell numbers make studying non-dividing or slow dividing cells (e.g., stem cells) easier than traditional culture methods (e.g., flasks, petri dishes, or well plates) due to the smaller sample volumes. (wikipedia.org)
Once the cells have differentiated into the desired type, the cellular material can be harvested from the controlled environment and prepared using conventional food processing and packaging methods. (usda.gov)
The scaffold allowed the researchers to significantly expand T cell numbers in a dish, compared with existing culture methods, and could bring T cell therapies, such as anti-cancer treatments, closer to clinical reality. (medgadget.com)

This paper presents results from a six-year ethnographic study involving interviews, focus groups and other forms of engagement with the scientific research team and other stakeholders, including public groups, which sought to understand respondents' reactions to cultured red blood cells. (stir.ac.uk)

To identify cell culture models supportive for Zika virus (ZIKV) replication. (wjgnet.com)
Various human and non-human cell lines were infected with a defined amount of ZIKV Polynesia strain. (wjgnet.com)
All investigated cell lines except CHO cells supported infection, replication and release of ZIKV. (wjgnet.com)
Here, we evaluated the semen of 49 ZIKV-infected men to identify immune factors correlating with long-term ZIKV shedding in semen and ZIKV-infected cell types in semen. (cdc.gov)
Additionally, we found ZIKV RNA in seminal leukocytes and epithelial cells. (cdc.gov)
In contrast to the frequent and prolonged shedding of ZIKV RNA in semen, the shedding of infectious ZIKV that could be cultured was rare, short-lived, and limited to the few samples with at least 7.0 log 10 ZIKV RNA copies per milliliter of semen," the authors write. (medscape.com)
None of the 75 cultured samples with RNA loads lower than 7.0 log 10 per milliliter yielded infectious ZIKV, and no sample that was obtained more than 30 days after illness onset yielded infectious ZIKV. (medscape.com)

For culturing of adherent cells, a range is limited within a scaffold in which the cells grow during the course of their division and proliferation, and therefore with continued proliferation it is unavoidable that they eventually reach the limit of proliferation in the scaffold. (justia.com)
miRNAs have been implicated as transcriptional regulators in a wide range of biological processes determining cell fate, stress response, proliferation, or death [ 5 ]. (hindawi.com)
Autologous preparations rich in growth factors promote proliferation and induce VEGF and HGF production by human tendon cells in culture. (springer.com)
HEMA reduces cell proliferation and induces apoptosis in vitro. (niom.no)

Researchers use organoid cultures in unique ways to study health and disease. (the-scientist.com)
Researchers at the Harvard Wyss Institute have developed a biomaterial scaffold that mimics the actions of antigen-presenting cells (APCs) in stimulating T cell growth and survival. (medgadget.com)
Researchers at the Harvard Wyss Institute took inspiration from another immune cell type, the APC, which stimulates T cells to grow and survive during an immune response. (medgadget.com)
The researchers designed a biomaterial scaffold that provides pro-survival and pro-growth biological cues to T cells, just like an APC in the body. (medgadget.com)
Using the scaffolds, the researchers were able to expand T cells from mice and humans much faster than existing culture techniques, and they confirmed that the expanded cells have clinical potential in a mouse lymphoma model. (medgadget.com)
Researchers from Lund have developed a completely animal-free preparation for cell culture, a medium that can be used to grow human cells, including for cancer research. (lu.se)

Interactions between light and various cells in cultures, such as bacteria or mammalian cells, are widely applied for optical sensors and optofluidic systems. (mdpi.com)

The epidemiological data to assist WHO in making recom- traditional method of influenza virus detection by isola- mendations on suitable virus strains for seasonal and tion in eggs or cell culture fol owed by antigenic typing pandemic influenza vaccines. (who.int)

Cell Stem Cell 19 , 569-572 (2016). (nature.com)
Nikon Corporation has great regard for the OptoSelect technology developed by BLI, as a result of which it invested $10 million in BLI in October 2016, and in February 2018, Nikon decided to invest an additional $30 million in the company. (nikon.com)

Our study suggests that TFs can decrease cell apoptosis, which may be mediated by altering the expression of miR-21, PTEN/Akt, and Bcl/Bax. (hindawi.com)
In murine cardiomyocytes miR-21 was found to protect from hypoxia/reoxygenation (H/R)-induced cell apoptosis via regulation of its target gene PDCD4 [ 13 ]. (hindawi.com)
Since epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors, including gefitinib (GEF) have been reported to induce the apoptosis of several cancer cell lines, in the present study, we examined whether the cytotoxic effects of GEF are further enhanced under amino acid starvation (AAS) culture conditions. (spandidos-publications.com)
PKC isozymes are LR-dependent molecules that link psychosine-induced LR disruption to reduced PKC function and altered cell signaling activity, possibly driving demyelination and apoptosis in oligodendrocytes and Schwann cells. (medscape.com)

Multiple sources have established the importance of osmolality when establishing cultivation conditions for stem cells to preserve optimal critical quality attributes (CQAs) like cell viability and productivity. (labmanager.com)

Here I show how at least some of these studies can be carried out in cultured HepG2 cells using Hox/luciferase reporters and a doxycycline-inducible expression system. (cam.ac.uk)

We study the effects on lung cell cultures using e. g. the NACIVT system and the array of characterization instruments of the aerosol lab in close collaboration with Occupational and Environmental Medicine and Lund Bioengineering and Regeneration. (lu.se)

The case of cultured red blood cells currently being grown in a laboratory for future use in human transfusion raises questions about the ontological status of such products of modern biotechnology. (stir.ac.uk)

This is a fact common to both culturing on flat surfaces such as standard plates, and three-dimensional culturing using a substrate. (justia.com)

Interferon α 2A (IFN-α 2A) belongs to the type I interferon protein family and is produced by many cell types in response to viral infection. (sigmaaldrich.com)
Cells were analyzed 48 h post infection for the amount of intracellular and extracellular viral genomes and infectious viral particles by quantitative real-time PCR and virus titration assay. (wjgnet.com)
Impact of Influenza A Virus Infection on Growth and Metabolism of Suspension MDCK Cells Using a Dynamic Model. (mpg.de)
Dynamics of intracellular metabolite pools in MDCK suspension cells during cell growth and influenza virus infection. (mpg.de)
Infection, Genetics and Evolution, 2018. (ac.ke)

A key component of microfluidic cell culture is being able to mimic the cell microenvironment which includes soluble factors that regulate cell structure, function, behavior, and growth. (wikipedia.org)

Brunner D, Frank J, Appl H, Schöffl H, Pfaller W, Gstraunthaler G. Serum-free cell culture: the serum-free media interactive online database. (springer.com)
Autologous human serum for cell culture avoids the implantation of cardioverter-defibrillators in cellular ardiomyoplasty. (springer.com)
The transport of serum amino acids into cells is an active process that is facilitated by plasma membrane-localized amino acid transporters. (spandidos-publications.com)
For the quantitative determination of 15(S)-HETE in culture supernatants, plasma, serum, and urine from any species. (enzolifesciences.com)
A purple well means the antibodies in the serum sample prevented poliovirus from infecting and killing cells within the cell culture. (cdc.gov)
The presence of antibodies in a serum sample, capable of blocking poliovirus from infecting cells, means that the person, from whom the sample was extracted, is protected from developing paralysis after exposure to poliovirus. (cdc.gov)

For example, eukaryotic cells have linear dimensions between 10 and 100 μm which falls within the range of microfluidic dimensions. (wikipedia.org)
Macroautophagy (thereafter designated as autophagy) is a self-digestive system conserved in all eukaryotic cells. (spandidos-publications.com)

In cell culturing, regardless of whether or not a cell culture support is used and regardless of whether suspended cells or adherent cells are used, supply of oxygen is an essential issue for achieving healthy growth of the cells, with the exception of anaerobic bacteria. (justia.com)

Moreover, with spheroid-forming cell population, an excessively large size is known to result in oxygen deficiency for the cells in the interior (NPL 1). (justia.com)
The dynamic micro-gravity spheroid 3D system, exhibits the ability to overcome many of the shortcomings of traditional 2D cell cultures. (norecopa.no)

The presence of necrostatin-1, an inhibitor of receptor-interacting serine/threonine-protein kinase 1 (RIPK‑1), but not that of Z-VAD-fmk, attenuated the cytotoxic effects of GEF under AAS culture conditions. (spandidos-publications.com)
They loaded the rods with Interleukin-2, a protein produced by APCs that can enhance T cell survival, and coated them using a lipid layer, to mimic the APC cell membrane. (medgadget.com)
A study by White et al (2009) found that psychosine's cytotoxic effects on oligodendroglia and Schwann cells was mediated through disruption of the architecture and composition of lipid rafts (cell membrane regions characterized by high cholesterol and sphingolipid concentration), followed by altered protein kinase C (PKC) function. (medscape.com)
Scientists also added various protein extracts from animals to make the cells grow. (lu.se)

Some of the major advantages of microfluidic cell culture include reduced sample volumes (especially important when using primary cells, which are often limited) and the flexibility to customize and study multiple microenvironments within the same device. (wikipedia.org)

As a result, cultures are exposed to more physiologically relevant conditions, increasing the predictive value of in vitro experiments. (genengnews.com)

NIH gave out $137 million in human and non human embryo stem cell research grants. (cbc-network.org)
Keep in mind that embryonic stem cell research isn't even at the level of any human clinical trials because those embryo stem cells keep forming tumors in the lab rats. (cbc-network.org)

Our aim was to establish a live cell culture to enable the understanding of the cellular processes driving the non-malignant growth of these tumors. (frontiersin.org)

This accumulation causes formation of globoid cells (hematogenous often-multinucleated macrophages containing undigested galactosylceramide), which is the histologic hallmark of Krabbe disease. (medscape.com)

Production in human HEK 293 cells offers authentic glycosylation. (sigmaaldrich.com)
Glycosylation contributes to stability in cell growth media and other applications. (sigmaaldrich.com)
Osmolality is a critical indicator of lactate production (influenced by pH) and media base addition, ultimately impacting glycosylation in cell culture. (labmanager.com)

To make food, a small number of cells are taken from the cell bank and placed in a tightly controlled and monitored environment (e.g., a very large, sealed vessel) that supports growth and cellular multiplication by supplying appropriate nutrients and other factors. (usda.gov)
Organs boast a unique three-dimensional cellular architecture, with cell-cell and cell-matrix interactions, creating a complex communication network through biochemical and mechanical signals. (norecopa.no)

Although the analyzed cell lines released comparable amounts of viral genomes to the supernatant significant differences were found for the number of infectious viral particles. (wjgnet.com)
Regardless of the transfection method, osmolality is important when transfecting cells with plasmid to initiate viral production. (labmanager.com)
Respiratory or enteric viral isolates obtained from PIM specimens using any of these cell lines are considered PIM. (cdc.gov)

If the well shows up as colorless, this means that antibodies did not block the virus from infecting and killing the cells. (cdc.gov)

For example, in a system that incorporates syringe pumps, channels for perfusion inlet, perfusion outlet, waste, and cell loading would need to be added for the cell culture maintenance. (wikipedia.org)
High cell density perfusion process for high yield of influenza A virus production using MDCK suspension cells. (mpg.de)
Semi-perfusion cultures of suspension MDCK cells enable high cell concentrations and efficient influenza A virus production. (mpg.de)

For example, for hollow fiber culturing, a liquid phase/gas phase exposure bioreactor has been proposed (PTL 3), as a system wherein hollow fibers inhabited by cells are rotated for alternate exposure to a gas phase and a liquid phase. (justia.com)
Need help designing a 194.0 °F bioreactor for cell culture. (electro-tech-online.com)
I've been having trouble deciding how to design a 194.0 °F (90ºC) bioreactor for cell culture (not cell culture now but I do need to get to that temperature to take a sample). (electro-tech-online.com)

The combination of the Beacon of BLI, employing the OptoSelect technology, with the live cell imaging technology of Nikon will expand the solutions already provided by Nikon in cell-related fields such as the development of antibody drugs and regenerative medicines. (nikon.com)

We show that it is possible to grow cells successfully with a completely defined and animal product-free medium," says Stina Oredsson. (lu.se)

Cell media osmolality must be controlled to optimize and maintain cell growth and vector production. (labmanager.com)
Falling out of specification could result in lower growth rate and viable HEK 293 cell concentrations, leading to growth inhibition and ineffective vector production [7]. (labmanager.com)
Higher osmolality has been used to increase the specific production rates of waste and/or slow cell growth to maximize productivity [10, 11]. (labmanager.com)
Highly efficient influenza virus production: A MDCK-based high-cell-density process. (mpg.de)
A Dynamic Model for Cell Growth, Metabolism & Virus Production of MDCK Suspension Cells. (mpg.de)
Evaluation of MDCK suspension cell lines for influenza A virus production: media, metabolism, and process conditions. (mpg.de)
Nikon will strengthen its position in cell-related fields by providing workflows for cell line development and production, from cell quality evaluation solutions and preclinical sorting of regenerative and genetic treatment cells to market expansion solutions. (nikon.com)

Anti-cancer T cell therapies involve culturing and sometimes modifying patient-specific T cells in a dish before administering them to a cancer patient, where they can attack and kill cancer cells. (medgadget.com)
In the right SEM image, T cells (in blue) bind to a section of a completed antigen-presenting cell-mimetic scaffold (in brown), where they are instructed to multiply and are kept alive for future use in T cell therapies. (medgadget.com)
Gene and cell therapies have the potential to revolutionize the treatment of incurable diseases that are often debilitating, if not fatal. (labmanager.com)

A major challenge with this technique lies in achieving sufficient T cell growth and survival in vitro to obtain a sufficient dose of reactive T cells. (medgadget.com)
The current gold standard in in vitro pre-clinical cancer treatment screening remain cell lines, grown on static flat surfaces - generally referred to known as traditional two-dimensional cultures (2D). (norecopa.no)

For the cell culture into the scaffold, it is necessary to establish certain conditions, such as the type of supplementation of the culture medium. (springer.com)
The members of the L-type amino acid transporter (LAT) family are Na + -independent transporters that deliver neutral amino acids into cells ( 2 ). (spandidos-publications.com)
Media vendors measure and report the osmolality range for their solutions, and it is common for scientists to check the osmolality of each media batch against specifications to ensure the proper environment for a specific cell line or type. (labmanager.com)
Oruru MB, Mugendi EN, Pasquet R, Runo S. Response of a wild-type and modern cowpea cultivars to arbuscular mycorrhizal inoculation in sterilized and non-sterilized soil. (ac.ke)

Data obtained from FluNe t (http://www.who.int/influenza/gisrs_laboratory/flunet/en/ ), a global web-based tool for influenza virological surveil ance and provided by National Influenza Centres (NICs) in the WHO GISRS network. (who.int)

Regulatory oversight of human foods comprised of or containing cultured animal cells depends on the animal species used as the original source of cultured cells, and it is based on the agencies' existing jurisdiction over products. (usda.gov)
Hsa_circ_001242 expression levels were significantly downregulated in four OSCC cell lines (SCC-9, SCC-15, SCC25, and CAL-27) than in human normal oral keratinocyte (HOK) cell lines. (hindawi.com)
The activity is determined by the dose-dependent cytotoxicity of the human TF-1 cell line (human erythroleukemic indicator cell line)) ≤ 0.4. (sigmaaldrich.com)
Here is a nice chart which shows that "dozens of human trials have already begun to assess the efficacy of adult stem cells in treating heart disease. (cbc-network.org)
Our results mean that growing human cells is closer to the environment in which they normally grow. (lu.se)

Subculturing is absolutely indispensable to promote cell growth and is an important process regardless of the purpose and method, but the operations of treating the cells with trypsin or collagenase to remove them from the scaffold, and reseeding them via a cell suspension, not only run the risk of contamination but are also time consuming, while stress exerted on the cells is another factor that cannot be overlooked. (justia.com)
It merges microfluidics, a set of technologies used for the manipulation of small fluid volumes (μL, nL, pL) within artificially fabricated microsystems, and cell culture, which involves the maintenance and growth of cells in a controlled laboratory environment. (wikipedia.org)
Scientists fiddle with formulas to boost the growth of their stem cell cultures. (the-scientist.com)
Whether it is later used in flasks or bioreactors, media must meet pre-determined osmolality specifications to ensure optimal cell growth and expansion [6]. (labmanager.com)
2 Cell strain: Culturing of a single cell into a clonal cell colony that is characterized by secretion, growth rate, cell size, and/or stability over a duration of time, by which clones of interest can be identified and exported for further expansion. (nikon.com)

Anatomy47

Organisms45

Diseases6

Chemicals and Drugs48

Analytical, Diagnostic and Therapeutic Techniques and Equipment63

Phenomena and Processes50

Disciplines and Occupations5

Technology, Industry, Agriculture7

Information Science3

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Proteins5

20212

Vivo8

Pluripotent stem4

Differentiation9

Substrates1

20192

Vero7

Monolayer2

Livestock and poultry1

Vaccine4

Viruses1

Neurons2

Epub6

Biology4

Tissue9

Genes2

Parkinson's Disease1

Derivation3

Fetal2

Monomer1

Genome1

Methods5

Reactions1

ZIKV7

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Researchers6

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20162

Apoptosis4

Viability1

Luciferase1

Characterization1

Biotechnology1

Substrate1

Infection5

Regulate1

Serum6

Eukaryotic cells2

Bacteria1

Spheroid2

Protein4

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Physiologically relevant1

Embryo stem2

Understanding1

Macrophages1

Glycosylation3

Cellular2

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Perfusion3

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Successfully1

Production7

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Type4

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Human5

Growth5