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  • Intracellular
  • We have shown previously that the ADP-ribosylation factor (ARF)-6 GTPase localizes to the plasma membrane and intracellular endosomal compartments.Expression of ARF6 mutants perturbs endosomal trafficking and the morphology of the peripheral membrane system.Furthermore, we have shown that the ARF6-containing intracellular compartment partially colocalized with transferrin receptors and cellubrevin and morphologically resembled the recycling endocytic compartment previously described in CHO cells. (nih.gov)
  • Here we demonstrate that at the lowest detectable levels of protein expression by cryoimmunogold electron microscopy, ARF6 localized predominantly to an intracellular compartment at the pericentriolar region of the cell. (nih.gov)
  • Furthermore, we have shown that the ARF6-containing intracellular compartment partially colocalized with transferrin receptors and cellubrevin and morphologically resembled the recycling endocytic compartment previously described in CHO cells. (nih.gov)
  • Accordingly, intracellular Na + levels were increased in ap1b1 mutant hair cells. (zfin.org)
  • Tissues
  • Explain the basic mechanisms of homeostasis by integrating the functions of cells, tissues, organs, and organ systems. (brandman.edu)
  • As these molecules are vital for life, metabolic reactions either focus on making these molecules during the construction of cells and tissues, or by breaking them down and using them as a source of energy, by their digestion. (wikipedia.org)
  • interactions
  • Collectively, these findings establish the existence of a differential vesicular trafficking mechanism for specific Usher protein variants in mouse cochlear hair cells, with the apical variants playing a potential role in endosomal recycling and stereocilia development/maintenance, and the basolateral variants involved in vesicle docking and/or fusion through SNAP25-mediated interactions. (biomedsearch.com)
  • antibodies
  • HEK-293 cells expressing ARF6 (T27N) were double labeled with antibodies against ARF6 (5 nm gold) followed by labeling with antisera against MPR (C, 10 nm gold), or with anti-ARF6 antibody (10 nm gold) followed by anti-Lamp-3 antibody (D, 5 nm gold). (nih.gov)
  • The cells were then exposed to antibodies against biotin followed by colloidal gold-conjugated antibodies and then critical-point dried. (umassmed.edu)
  • Endosomes
  • However, another study on the distribution of ARF6 in subcellular fractions of Chinese hamster ovary (CHO) cells suggested that ARF6 did not localize to endosomes labeled after 10 min of horseradish peroxidase (HRP) uptake, but instead was uniquely localized to the plasma membrane, and that its reported endosomal localization may have been a result of overexpression. (nih.gov)
  • subcellular
  • Cell death pathways are likely regulated in specialized subcellular microdomains, but how this occurs is not understood. (umassmed.edu)
  • Hair Cells
  • Immature mouse cochleae and UB/OC-1 cells were used in this work to address whether specific variants of PCDH15 and VLGR1 are being selectively transported to opposite poles of the hair cells. (biomedsearch.com)
  • The hair cells of the inner ear are polarized epithelial cells with a specialized structure at the apical surface, the mechanosensitive hair bundle. (zfin.org)
  • Mechanically-evoked calcium transients were reduced in mutant hair cells, indicating that mechanotransduction was also compromised. (zfin.org)
  • We observed that the Na + /K + -ATPase pump (NKA) was less abundant in the basolateral membrane and was mislocalized to apical bundles in ap1b1 mutant hair cells. (zfin.org)
  • Our results suggest that Ap1b1 is essential for maintaining integrity and ion homeostasis in hair cells. (zfin.org)
  • Plant
  • Plant, Cell & Environment, 31: 1510-1521. (wiley.com)
  • Cell Processes: Photosynthesis Part 1: Pigments in Plant Leaves Purpose: The purpose of this experiment is to visualize the pigments present in plant leaves by using paper chromatography. (educationindex.com)
  • mitochondrial
  • Cells with any of the gag mutations displayed aberrant mitochondrial morphology characterized by elongated, unbranched tubes and highly fenestrated structures. (nih.gov)
  • Additionally, each of the gag mutations prevented mitochondrial fragmentation caused by loss of the mitochondrial fusion factor, Fzo1p, or by treatment of cells with sodium azide. (nih.gov)
  • type
  • CHO cells expressing wild-type ARF6 were doubled labeled with an anticellubrevin antibody (15 nm gold), followed by anti-Tfn-R antibody (A, 10 nm), or with an anti-ARF6 antibody (5 nm gold) followed by an anticellubrevin antibody (B, 10 nm gold). (nih.gov)
  • Energy
  • Overall, HK3 may promote cell survival by controlling ROS levels and boosting energy production. (wikipedia.org)
  • Several
  • In this study, the metal compartmentation, elemental composition of zinc deposits and zinc complexation within leaves from poplars grown on soil with mixed metal contamination was analysed combining several histochemical and microanalytical approaches. (deepdyve.com)
  • potential
  • Purpose: My purpose is to provide information on cell phone radiation, the risks that can be caused by cell phone radiation, and the potential health problems that could result from this. (educationindex.com)