Cell cell and protein protein receptor clone cells. Medical search. Frequent questions

To clone a human CTR (hCTR), a BIN-67 cDNA library was screened using a cDNA probe from a porcine renal CTR (pCTR) that we recently cloned. (jci.org)
Transfection of this cDNA into COS cells resulted in expression of receptors with high affinity for salmon CT (Kd = approximately 0.44 nM) and for human CT (Kd = approximately 5.4 nM). (jci.org)
The cloned cDNA encodes a putative peptide of 490 amino acids with seven potential transmembrane domains. (jci.org)
A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains. (jax.org)
FAS was initially purified and cloned from SKW6.4 cells and a cDNA library of human T cell lymphoma KT-3 cells. (biolegend.com)
We and other groups discovered the human IL-17F gene from a human EST sequence, a genomic DNA clone, and T-cell cDNA sequences in 2001 [ 3 , 8 , 9 ]. (hindawi.com)
As a result of alternative splicing of the Epo R gene, cDNA clones encoding truncated soluble forms of Epo R have been found. (rndsystems.com)

IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. (rndsystems.com)
Cloning the neurokinin receptor genes in guinea pig epithelial cells and their expression in epithelial cells lines would allow study of their function. (usda.gov)
In contrast, the previously established ER-negative TMX2-28 cell line has decreased expression of both DOK7 and ELF5 and increased DNA methylation in the transcriptional start site region of these genes. (umass.edu)
Cells deficient in both these proteases are much more amenable to the production of proteins from cloned genes. (neb.com)
We mapped all estrogen receptor and RNA polymerase II binding sites on a genome-wide scale, identifying the authentic cis binding sites and target genes, in breast cancer cells. (nature.com)
Fully human monoclonal antibodies are produced using transgenic mice that contain human immunoglobulin genes or using phage display (ie, a bacteriophage-based cloning method) of immunoglobulin genes isolated from human B cells. (msdmanuals.com)

Endothelial protein C receptor (EPCR) also known as activated protein C receptor (APC receptor) is a protein that in humans is encoded by the PROCR gene . (wikidoc.org)
The protein encoded by this gene is a receptor for protein C that enhances its activation. (wikidoc.org)
Gene expression and cellular localization of neurokinin receptors would be examined by reverse transcription-polymerase chain reaction and in situ hybridization. (usda.gov)
Deletion of this gene confers resistance to this type of phage, but does not significantly affect the transformation or growth characteristics of the cell. (neb.com)
The glmS gene (glucosamine synthetase) is mutated and the expressed GlmS protein (67 kDa) contains six histidine to alanine substitutions (positions 62, 65, 432, 436, 466, 467). (neb.com)
The isolation of temperature-sensitive transformation-defective mutants of Rous sarcoma virus led to the hypothesis that the viral protein or proteins should be temperature-sensitive in its expression or function, and Dr. John Wyke and Rohrschneider found that expression of some of the cell-surface tumor antigens correlated with transformation, suggesting that they were under control of the transforming gene. (fredhutch.org)
The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. (sigmaaldrich.com)
By this technique, the gene coding for restin (RSN) was assigned to chromosome region 12q24.31→q24.33, while localization of the α-2-macroglobulin receptor (A2MR) was refined to 12q13.1→q13.3. (karger.com)
To further analyze the restin gene, a 500-kb YAC clone containing the gene was isolated and analyzed. (karger.com)
Molecular cloning and domain structure of human myosin-VIIa, the gene product defective in Usher syndrome 1B. (embl-heidelberg.de)
DNA constructs were cloned into a pGL3 promoter vector for luciferase gene reporter assays. (cdc.gov)
The Hansenula polymorpha PER9 gene encodes a peroxisomal membrane protein essential for peroxisome assembly and integrity. (microbiologyresearch.org)
This gene encodes a B-lymphocyte surface molecule that plays a role in the development and differentiation of B-cells into plasma cells . (wikidoc.org)
Alternative splicing of this gene results in two transcript variants that encode the same protein. (wikidoc.org)
Figure 1: Summary of estrogen receptor and RNA PolII binding sites and correlation with nucleotide and gene number. (nature.com)
Figure 3: Estrogen receptor and RNA PolII binding relative to specific gene targets. (nature.com)
In the version of this article initially published online, the estrogen receptor and RNA PolII binding data were aligned incorrectly in the top panel of Figure 3, which represents estrogen receptor binding at the ESR1 gene region. (nature.com)
The resultant DNA is placed in a mammalian cell culture, which then expresses the resultant gene, producing the desired antibody. (msdmanuals.com)

Specifically, cell-mediated responses typically focus on to major histocompatibility complex (MHC) class II mol- peptides from internal influenza proteins, which are far less ecules. (cdc.gov)
Emerging evidence indicates that findings from from PR8 that are processed to generate peptides recog- mouse studies are pertinent to immunopathology in human nized by influenza-specific CD8+ T cells. (cdc.gov)
The endothelins ET-1 ET-2 and ET-3 are vasoactive peptides originally cloned from endothelial cells [1] but also made by various other cell types including Taladegib some tumor cells [2-5]. (exposed-skin-care.net)
Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. (sigmaaldrich.com)
We found that most of the previously described PAR 1 -targeted agents can also activate/desensitize PAR 2 , and most of these peptides can also activate a calcium signaling pathway in a target cell that possesses PAR 2 along with PAR 1 . (aspetjournals.org)
In contrast, thrombin receptor-activating peptides derived from the human PAR 1 receptor sequence (e.g. (aspetjournals.org)

Teplizumab, sold under the brand name Tzield, is a monoclonal antibody, a type of antibody manufactured by cloning a white blood cell that was exposed to a target protein. (yaledailynews.com)
ADCP (antibody-dependent cellular phagocytosis) assays were performed using human leukemia monocytic cells (THP-1). (news-medical.net)
Current vaccine strategies against influenza focus on Antigen-specific ligation of T-cell receptors induces generating robust antibody responses. (cdc.gov)
This Anti-Dopamine Transporter Antibody, N-terminus, clone DAT-Nt is validated for use in IC, IH, WB for the detection of Dopamine Transporter. (sigmaaldrich.com)
The T45-2342 monoclonal antibody recognizes the mouse F4/80 antigen which is also known as EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1). (bdbiosciences.com)
Obese mice administered anti-B cell CD-20 antibodies, however, did not become less responsive to insulin and as a result did not develop diabetes mellitus or the metabolic syndrome, the posited mechanism being that anti-CD20 antibodies rendered the T cell antibodies dysfunctional and therefore powerless to cause insulin desensitivity by a B cell antibody-modulated autoimmune response. (wikidoc.org)
The 1A8 antibody reacts with Ly-6G, a 21 - 25 kDa GPI-anchored protein, which together with the structurally related Ly-6C protein comprises the granulocyte receptor-1 antigen (Gr-1). (stemcell.com)
The 1A8 antibody binds specifically to Ly-6G, whereas another commonly used antibody, clone RB6-8C5, binds to both Ly-6G and Ly-6C. (stemcell.com)
It has been reported that the 1A8 antibody detects Ly-6G-expressing granulocytes in peripheral blood, whereas the RB6-8C5 antibody also binds to Ly-6C-expressing lymphocytes, monocytes, and dendritic cells. (stemcell.com)
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse Neutrophil Enrichment Kit (Catalog #19762). (stemcell.com)
Murine monoclonal antibodies are produced by injecting a mouse with an antigen, harvesting its spleen to obtain B cells that are producing antibody specific to that antigen, fusing those cells with immortal mouse myeloma cells, growing these hybridoma cells (eg, in cell culture), and harvesting the antibody. (msdmanuals.com)
In both cases, the process usually begins as above with production of mouse hybridoma cells that make antibody to the desired antigen. (msdmanuals.com)

We have examined the T cell receptor (TcR) expression of clones specific for epitopes of mycobacterial 65-kDa heat-shock protein (hsp65) in the context of two different HLA molecules, and used this system as a model to assess the selection of T cells responsive to this antigen in vivo. (ox.ac.uk)
Sequencing alpha and beta chains of representative V beta 5.2+ and V beta 5.6+ PB-derived clones revealed TcR which were identical to those utilized by the SF-derived clones, showing that the repertoire for recognition of this antigen is stable over time. (ox.ac.uk)
EPCR protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. [3] It belongs to the MHC class I / CD1 family of proteins, that is characterized by having a deep groove, that in other proteins in the family (but not in EPCR) is usually used for antigen binding. (wikidoc.org)
Influenza-specific CD8+ T cells recognize multiple of reagents and genetically modified mouse models has viral epitopes on target cells and antigen-presenting cells. (cdc.gov)
Fig. 3: Analysis of libraries of CD38 + CCR7 - T cell clones and fluorescence barcoding to dissect the antigen-specific repertoire of effector CD4 + T cells. (nature.com)
B-lymphocyte antigen CD20 or CD20 is an activated- glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase ( CD45 R+, CD117 +) and progressively increasing in concentration until maturity. (wikidoc.org)
Antigen-specific T cell unresponsiveness in cloned helper T cells mediated via the CD2 or CD3/Ti receptor pathways. (ox.ac.uk)
We have investigated the role of the CD2 protein in the negative regulation of immune function and report that similar to antigen and anti-CD3, the monoclonal anti-CD2 antibodies (T112 and T113) can induce specific unresponsiveness. (ox.ac.uk)
Therefore, early in the formation of oral lichen planus lesions, CD8 + T cells may recognize an antigen associated with the major histocompatibility complex (MHC) class I on keratinocytes. (medscape.com)
After antigen recognition and activation, CD8 + cytotoxic T cells may trigger keratinocyte apoptosis. (medscape.com)
Chimeric monoclonal antibodies activate antigen-presenting cells (APCs) and T cells more effectively than murine monoclonal antibodies but can still induce production of human anti-chimeric antibodies. (msdmanuals.com)

these receptors comprise a distinct family of G protein-coupled seven transmembrane domain receptors. (jci.org)
Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. (rndsystems.com)
Thus, the IL-17 cytokine family appears to represent a distinct ligand-receptor signaling system. (hindawi.com)
Functionally distinct T-cell over the course of a year, vaccine strains must be reformu- subsets are broadly identified according to their differen- lated specifically for each influenza season. (cdc.gov)
The influenza A viruses infect host serologically distinct coat proteins. (cdc.gov)
Four distinct complement receptors, CR1, CR2, CR3, and CR4, have been described for the surface-bound complement fraction C3 and its cleavage fragments. (medscape.com)

Protein C is an anti-coagulant serine protease activated by the blood coagulation pathway. (wikidoc.org)
We have recently identified, for the first time, that bronchial epithelial cells are its novel cell source in response to IL-33 via ST2-ERK1/2-MSK1 signaling pathway. (hindawi.com)
In HEK293 cells solid potentiation of TRPV1 like this induced by ET-1 via the ETAR could possibly be induced by PKC activation however not with activators from the adenylyl cyclase or the PKA pathway. (exposed-skin-care.net)
Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. (sigmaaldrich.com)
Efficient transport of cell surface glycoproteins to the Golgi apparatus has been previously demonstrated for a limited number of proteins, and has been proposed to require selective sorting in the endocytic pathway after internalization. (rupress.org)
These results suggest that one of the functions of late endosomes is constitutive recycling of cell surface receptors through the Golgi apparatus if they fail to recycle to the cell surface directly from early endosomes, and that the late endosome recycling pathway is followed frequently by many rapidly internalized proteins. (rupress.org)

A soluble version of the type II receptor is induced by anti-inflammatory agents such as glucocorticoids, IL-4 and IL-13. (rndsystems.com)

Interestingly, the hCTR sequence is remotely related to the cAMP receptor of Dictyostelium discoideum (21% identical), but is not significantly related to other G protein-coupled receptor sequences now in the data bases. (jci.org)
We show also that unstable RAR-RXR heterodimers were formed in solution, and that C-terminal sequences and the DNA-binding domains of both receptors were required for efficient formation of stable heterodimers on response elements. (nih.gov)
For confirming the S protein-specificity of IgG4-expressing B lymphocyte clones, four mAbs (monoclonal antibodies) were cloned from the BCR (B cell receptor) sequences, and their RBD binding and S protein-binding were assessed. (news-medical.net)

The third aim is to determine whether substance P activates neurokinin receptors on epithelial cells to stimulate chloride secretion and prostaglandin synthesis. (usda.gov)
Hs 578T are epithelial cells isolated from breast tissue derived from a 74-year-old, White, female breast cancer patient. (atcc.org)
This distinction is of epithelial cells by attaching to a cellular receptor (sialic consequence since many viruses rapidly mutate their coat acid) by the viral surface protein hemagglutinin (HA). (cdc.gov)
Current data suggest that oral lichen planus (OLP) is a T-cell-mediated autoimmune disease in which autocytotoxic CD8 + T cells trigger the apoptosis of oral epithelial cells. (medscape.com)

IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. (rndsystems.com)
Fas-activated DCs increase the expression of MHC II, B7 co-stimulatory molecules, and DC-lysosome-associated membrane proteins (DC-LAMP), and secrete proinflammatory cytokines such as IL-1β and TNF-α. (biolegend.com)
These three cytokines are differentially expressed in activated CD4 + T cells. (hindawi.com)
We discuss the advantages of develop- ing a vaccine based on cell-mediated immune responses tion is not absolute, since CD4+ cells may exhibit lytic toward highly pathogenic influenza virus and potential activity, while CD8+ cells secrete antiviral cytokines, problems arising from immune pressure. (cdc.gov)
Activated CD8 + T cells (and possibly keratinocytes) may release cytokines that attract additional lymphocytes into the developing lesion. (medscape.com)
Les numérations plaquettaire et leucocytaire ainsi que la concentration en cytokines (IL-2R, IL-8 et TNF-) ont été me- surées. (who.int)

Cloning, characterization, and expression of a human calcitonin receptor from an ovarian carcinoma cell line. (jci.org)
Restricted T cell receptor expression by human T cell clones specific for mycobacterial 65-kDa heat-shock protein: selective in vivo expansion of T cells bearing defined receptors. (ox.ac.uk)
Similar studies of TcR expression were carried out on hsp65-specific, DP4-restricted clones derived from the SF of a patient with rheumatoid arthritis by two independent cloning procedures. (ox.ac.uk)
Other unusual features of the receptor include its ability to engage classes of molecules, rather than individual ligands, and its enhanced surface expression in environments rich in RAGE ligands. (jci.org)
This study characterizes the expression of hormone receptors, and the mRNA and DNA methylation levels of docking protein 7 (DOK7), and E74-like factor 5 (ELF5), in 21 novel tamoxifen-resistant cell lines and extends the findings to primary and recurrent human breast tumors. (umass.edu)
The parent (MCF-7) and tamoxifen-selected cell lines were characterized for protein expression of ER, progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2) using immunohistochemistry (IHC). (umass.edu)
Expression of DOK7 and ELF5 is significantly up-regulated in half of the tamoxifen-selected cell lines as compared to the parental MCF-7. (umass.edu)
The properties of this strain that contribute to its usefulness as a protein expression strain are described below. (neb.com)
Thus DE3 strains may exhibit uninduced target protein expression. (neb.com)
Within this research we looked into ET receptor expression in DRG and using the patch clamp technique the effects of ET-1 on responses to capsaicin in DRG neurons. (exposed-skin-care.net)
Results Endothelin receptors in dorsal root ganglion neurons The expression of endothelin receptor subtypes in the rat lumbar DRG was analyzed in binding experiments using 125I-ET-1 as the radioligand. (exposed-skin-care.net)
Two-color analysis of the expression of Ly-49C/I on splenic NK cells. (bdbiosciences.com)
The proportion of NK T cells expressing Ly-49C/I is higher (2-5 fold) in thymus than in liver (immature and mature NK T cells, respectively), and there is evidence that the down-regulation of Ly-49 receptor expression is necessary for normal NK T-cell development. (bdbiosciences.com)
Three-color image analysis of F4/80 expression by cells in C57BL/6 mouse spleen. (bdbiosciences.com)
Members of this nascent protein family are characterized by common structural features and similar intron / exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. (wikidoc.org)
Defects in the expression of complement or complement receptors may result in loss of tolerance to self-proteins and the development of immune complex-mediated autoimmune diseases such as systemic lupus erythematosus (SLE). (medscape.com)
Among healthy individuals, the number of CR1 receptors on the red blood cells varies widely, yet the phenotypic expression, regulated by 2 codominant alleles, is stable. (medscape.com)
Younes F, Quartey EL, Kiguwa S, Partridge M. Expression of TNF and the 55-kDa TNF receptor in epidermis, oral mucosa, lichen planus and squamous cell carcinoma. (medscape.com)

In conclusion, it is deduced that upregulation of autophagy contributed to the AT1-AA-induced ß-cell apoptosis and islet dysfunction, and AT1R mediated the signal transduction. (bvsalud.org)

The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. (rndsystems.com)
FAS is a type I transmembrane protein and belongs to the TNF receptor superfamily. (biolegend.com)
The Ly-49 family of NK-cell receptors are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate-recognition domains (CRD) that bind to MHC class I alloantigens. (bdbiosciences.com)

The mutated GlmS protein does not bind Ni-NTA resin in the presence of 20mM imidazole binding/ wash buffer, whereas wt GlmS protein binds Ni-NTA resin and is not eluted until the imidazole concentration is within 55-80 mM (1). (neb.com)
The CR1 receptor preferentially binds C3b that is covalently attached to immune complexes, and it has a weaker affinity for bound C4b and iC3b. (medscape.com)

Inhibition of cytotoxic effect of FASL on Jurkat cells in the presence of recombinant human FAS. (biolegend.com)
The 397 amino acid recombinant protein has a predicted molecular mass of approximately 44.7 kD. (biolegend.com)
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. (biolegend.com)
GCG (AAH05278, 1 a.a. ~ 180 a.a) full-length recombinant protein with GST tag. (sigmaaldrich.com)

We review the literature express CD8 and induce apoptosis of cells on which they on the role of CD4+ and CD8+ T cell-mediated immunity in recognize foreign antigens presented by MHC class I mol- influenza infection and the available data on the role of ecules, providing a defense against intracellular pathogens these responses in protection from highly pathogenic such as viruses. (cdc.gov)
We sought to extend the receptor-desensitization paradigm for use with a cultured cell system, in which an intracellular calcium signal rather than a contractile response might be used as an index of receptor activation. (aspetjournals.org)
The microtubule-based kinesin motors and actin-based myosin motors generate movements required for intracellular trafficking, cell division, and muscle contraction. (embl-heidelberg.de)

Binding of these ligands to RAGE does not accelerate clearance or degradation but rather begins a sustained period of cellular activation mediated by receptor-dependent signaling. (jci.org)
This last point is crucial, since it explains how upregulation of this receptor can contribute to an ascending spiral of RAGE-dependent cellular perturbation. (jci.org)
Taken together, these features of RAGE allow the receptor to propagate cellular dysfunction in a number of pathophysiologically relevant situations, most often dictated by the formation and persistence of ligands in the tissues. (jci.org)
As described below, these diverse situations range from the complications of diabetes and cellular perturbation in amyloidoses to immune and inflammatory responses and tumor cell behavior. (jci.org)
CITE (cellular indexing of transcriptomes and epitopes)-sequencing was performed for labeling anti-S and anti-S RBD (receptor-binding domain)-binding B lymphocytes. (news-medical.net)
This property gives vaccines a well-developed experimental system to analyze T that induce protective cellular immune responses the cell-mediated immunity. (cdc.gov)
Around this time, Dr. Chuck Sherr at St. Jude's and Dr. Richard Stanley at Albert Einstein identified the normal cellular Fms protein as the cell surface receptor for a growth factor known as the macrophage colony-stimulating factor or M-CSF. (fredhutch.org)
Under prolonged cellular stresses, the σR1 translocates to the extended ER network, whereby it interacts with and regulates the function of a variety of ion channels, receptors, or kinases. (molvis.org)
Protein tyrosine (pTyr) phosphorylation is a common post-translational modification which can create novel recognition motifs for protein interactions and cellular localisation, affect protein stability, and regulate enzyme activity. (embl.de)
Consequently, maintaining an appropriate level of protein tyrosine phosphorylation is essential for many cellular functions. (embl.de)
The complement system, interacting with its regulatory molecules and cellular receptors, plays a central role in the induction and regulation of immunity. (medscape.com)
They are single-chain molecules present on host cellular membranes and belong to the complement control protein family. (medscape.com)

Structurally, the IL-17 cytokine family members have no sequence similarity to any other known cytokine or other mammalian proteins [ 2 ]. (hindawi.com)

CD20 is the target of the monoclonal antibodies rituximab , ocrelizumab , obinutuzumab , ofatumumab , ibritumomab tiuxetan , tositumomab , and ublituximab , which are all active agents in the treatment of all B cell lymphomas , leukemias , and B cell-mediated autoimmune diseases. (wikidoc.org)

The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. (rndsystems.com)
The estrogen receptor is the master transcriptional regulator of breast cancer phenotype and the archetype of a molecular therapeutic target. (nature.com)

Mammalian Cdc25 phosphatase is responsible for the dephosphorylation of Cdc2 and other cyclin-dependent kinases at Thr14 and Tyr15, thus activating the kinase and allowing cell cycle progression. (embl.de)

A human ovarian small cell carcinoma line (BIN-67) expresses abundant calcitonin (CT) receptors (CTR) (143,000 per cell) that are coupled, to adenylate cyclase. (jci.org)
The dissociation constants (Kd) for the CTRs on these BIN-67 cells is approximately 0.42 nM for salmon CT and approximately 4.6 nM for human CT. (jci.org)
The purified protein is a human retinoid X receptor beta (hRXR beta). (nih.gov)
Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines. (rndsystems.com)
In addition, FAS/FASL interaction increases the production of chemokines in NK-T cells, and FASL acts as a survival factor for human CD34 + cells and increases their colony formation. (biolegend.com)
Human FAS, amino acids (Arg17-Asn173) (Accession# NM_000043), was expressed in 293E cells. (biolegend.com)
To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
Western blot: Recognizes a diffuse band at approximately 70-85 kDa on western blots of extracts (20 ug total protein) from human caudate, putamen, and nucleus accumbens. (sigmaaldrich.com)
We developed a calcium signaling-based assay, using cultured human embryonic kidney cells (HEK), that evaluates simultaneously, the activation/desensitization or blockade of the proteinase-activated receptors, PAR 1 and PAR 2 . (aspetjournals.org)
The abilities of the PAR 1 -targeted agents to desensitize or block PAR 1 in the HEK cell assay were compared with their activities in a human platelet aggregation assay. (aspetjournals.org)
To this end, we developed a calcium-signaling assay, employing cultured human embryonic kidney cells (HEK293) in which the action of PAR 1 and PAR 2 agonists and antagonists could be evaluated simultaneously. (aspetjournals.org)
The biological effects of Epo are mediated by the Erythropoietin receptor (Epo R). At the protein sequence level, the human Epo R is approximately 83% identical to the mouse protein. (rndsystems.com)
Because the described cDNAs encode less than half of the protein predicted from immunoblots, we have cloned cDNAs encoding the rest of human myosin-VIIa. (embl-heidelberg.de)
We aimed to establish scoring criteria for the implementation of ANA IFT on human epithelioma-2 (HEp-2) cells and ELISA-based testing. (bvsalud.org)

The interaction of FAS/FASL in immature dendritic cells (DCs) induces functional maturation. (biolegend.com)
The results suggest that exposure to GLA, even in low doses, during infantile period in the rat induces alterations in the kainic acid receptor in the brain. (go.jp)
However, it remains unclear whether AT1-AA induces ß-cell apoptosis and participates in the development of DM. (bvsalud.org)

The OmpT protease resides at the surface of wild type E. coli in both K-12 and B strains, presumably helping the cells to derive amino acids from their external environment. (neb.com)
Rohrschneider focused initially on cell-surface proteins in cells infected with avian oncorna viruses, identifying the envelope and group-specific antigens (gag proteins). (fredhutch.org)
This was one of the initial discoveries that mutations in a cell surface receptor could initiate oncogenesis, just one year after the avian erythroblastosis virus oncogene was found to be a mutant EGF receptor. (fredhutch.org)
The cation-independent mannose 6-phosphate receptor and the low density lipoprotein receptor, both rapidly internalized from the cell surface, and the synaptic vesicle membrane protein synaptophysin, were transported to the Golgi apparatus with equivalent, nonlinear kinetics. (rupress.org)
Since each of the transmembrane proteins studied showed the same t1/2 for transport to the Golgi apparatus, we conclude that transport of these proteins from the cell surface to the Golgi apparatus does not require sorting information specific to any one of these proteins. (rupress.org)
However, in both instances interleukin 2 receptor surface levels were increased. (ox.ac.uk)

Gr-1 is expressed on monocytes, neutrophils and subsets of macrophages, plasmacytoid dendritic cells, and T cells. (stemcell.com)
Mice with C5a receptor-deficient dendritic cells promote induction of Treg and Th17 cells. (medscape.com)
It is a single-chain membrane glycoprotein of approximately 200 kd that has 4 allotypic forms on myeloid cells (eg, erythrocytes, granulocytes, monocytes), lymphoid cells (mostly B cells), follicular dendritic cells, and glomerular podocytes. (medscape.com)

Partial or complete deficiencies of the components of the complement system, including its receptors and regulatory proteins, are now described in humans and may be of a genetic or familial origin or acquired. (medscape.com)
The receptors are widely expressed in humans. (medscape.com)

Some of the receptor PTPases contain fibronectin type III (FN-III) repeats, immunoglobulin-like domains, MAM domains or carbonic anhydrase-like domains in their extracellular region. (embl.de)
Effector proteins interacting with the Fc portion of immunoglobulin M (IgM) include complement and complement receptors. (medscape.com)

Three murine members of the RXR family (mRXR alpha, beta, and gamma) have also been cloned, and their interactions with RARs and TRs have been investigated. (nih.gov)
Therefore, murine airway CD8 + T cell-responses to aspiration of Aspergillus conidia may be mediated in part by the ability of conidia to germinate in the host lung tissue. (cdc.gov)

The dense subepithelial mononuclear infiltrate in oral lichen planus is composed of T cells and macrophages, and there are increased numbers of intraepithelial T cells. (medscape.com)
[ 8 ] T cells in oral lichen planus contain mRNA for TNF and secrete TNF in vitro. (medscape.com)
The role of autoimmunity in the pathogenesis is supported by many autoimmune features of oral lichen planus, including its chronicity, onset in adults, predilection for females, association with other autoimmune diseases, occasional tissue-type associations, depressed immune-suppressor activity in patients with oral lichen planus, and the presence of autocytotoxic T-cell clones in lichen planus lesions. (medscape.com)
Sugerman PB, Satterwhite K, Bigby M. Autocytotoxic T-cell clones in lichen planus. (medscape.com)
Shan J, Ma JM, Wang R, Liu QL, Fan Y. Proliferation and Apoptosis of Peripheral Blood Mononuclear Cells in Patients with Oral Lichen Planus. (medscape.com)

Substance P which is found in extrinsic and intrinsic neurons is elevated along with its receptor during inflammation. (usda.gov)
The first aim addresses whether neural secretory reflexes triggered by mucosal stroking and activation of 5-HT1P receptors present on intrinsic afferent neurons stimulate chloride secretion. (usda.gov)
Background Endothelin-1 (ET-1) both stimulates nociceptors and sensitizes these to noxious stimuli an impact probably mediated with the ETA receptor (ETAR) expressed in sensory neurons. (exposed-skin-care.net)
ET-1 highly potentiated capsaicin-induced TRPV1 currents in a few neurons and in HEK293 cells co-expressing TRPV1 as well as the ETAR. (exposed-skin-care.net)

The type II receptor acts as a decoy target for IL-1, inhibiting IL-1 activities by preventing the binding of IL-1 to the type I receptor. (rndsystems.com)
Autoantibody against the angiotensin II type I receptor (AT1-AA) has been found in the serum of patients with diabetes mellitus (DM). (bvsalud.org)

These findings suggest a convergence of the signaling pathways of some members of the nuclear receptor superfamily. (nih.gov)
The receptor for IL-17F is the heterodimeric complex of IL-17RA and IL-17RC, and IL-17F activates many signaling pathways. (hindawi.com)
Endothelins action on ETA and ETB receptors (ETARs and ETBRs) [6 7 both G protein-coupled receptors that may activate multiple G proteins types and impact several signaling pathways [8]. (exposed-skin-care.net)
To investigate the signaling pathways involved in potentiation we analyzed the effects of ET-1 in HEK293 cells coexpressing the ETAR and TRPV1. (exposed-skin-care.net)

2001). "Identification of the protein C/activated protein C binding sites on the endothelial cell protein C receptor. (wikidoc.org)
Under physiological circumstances, the resistance of the endothelial cell lining to interactions with platelets and coagulation factors prevents thrombosis. (medscape.com)

Our results identify immune-dominant, serovar-specific, and cross-reactive T cell antigens, which should aid in the design of T cell-vaccination strategies against Salmonella . (nature.com)
Fig. 5: HlyE and CdtB are S . Typhi- and S . Paratyphi A-specific immunodominant T cell antigens. (nature.com)
The protein has no known natural ligand [4] and its function is to enable optimal B-cell immune response, specifically against T-independent antigens. (wikidoc.org)

Overview of the Immune System The immune system distinguishes self from nonself and eliminates potentially harmful nonself molecules and cells from the body. (msdmanuals.com)

The cDNAs for Epo have been cloned from many species. (rndsystems.com)

In an initial conference survey we demonstrated that activation from the ETAR potentiated TRPV1 replies to capsaicin in HEK 293 cells [24]. (exposed-skin-care.net)
Furthermore, we used this assay to develop a PAR 1 receptor-activating probe [Ala-parafluoroPhe-Arg-Cha-Cit-Tyr-NH 2 (Cit-NH 2 )], which displays a high degree of specificity for PAR 1 over PAR 2 , and we used the assay to quantitate the ability of trypsin to disarm the activation of PAR 1 by thrombin. (aspetjournals.org)
[ 2 ] T cell-derived complement C3a and CD46 activation is required for Th1 induction. (medscape.com)
The density of CR1 receptors on cell surfaces varies with the cell type and with the activation of the cell for neutrophils and monocytes. (medscape.com)
Platelet activation allows binding of these proteins, which bridges adjacent platelets. (medscape.com)

Sorting and function of peroxisomal membrane proteins. (microbiologyresearch.org)
PEX3 functions as a PEX19 docking factor in the import of class I peroxisomal membrane proteins. (microbiologyresearch.org)

another ER chaperone), and becomes available to regulate inositol trisphosphate (IP3) receptor-mediated Ca 2+ release to maintain mitochondrial Ca 2+ homeostasis. (molvis.org)

Those first immunoprecipitation experiments also revealed a related protein in uninfected cells, one of the first sightings of the Src proto-oncogene product. (fredhutch.org)
He also went proto-oncogene hunting, cloning out the normal cell version of the Fms oncogene and identifying the mutations that make it oncogenic. (fredhutch.org)

IL-17a-producing gammadeltaT cells and NKG2D signaling mediate bacterial endotoxin-induced neonatal lung injury: implications for bronchopulmonary dysplasia. (jax.org)

Our data illustrate the usefulness of the HEK cell assay for evaluating the PAR 1 /PAR 2 selectivity of PAR-activating agonists. (aspetjournals.org)

There was conservation of alpha chain usage, since all clones expressed a member of the V alpha 1 family, but again CDR3 sequence conservation was not apparent. (ox.ac.uk)
The four conserved cysteines in the C-terminal half of the IL-17F sequence are shown to form a cystine knot structural motif in the crystal structure, and, interestingly, this cystine knot structure is similar to a common structural motif seen in several growth factors, such as bone morphogenic proteins (BMPs), TGF- β , nerve growth factor (NGF), and platelet-derived growth factor (PDGF) [ 9 ]. (hindawi.com)
Fig. 6: Sequence-dependent recognition of different Salmonella serovars by CdtB-specific T cell clones. (nature.com)
The mature proteins from the various species are highly conserved, exhibiting greater than 80% sequence identity at the amino acid level. (rndsystems.com)

Fig. 7: Characterization of the specificity of T cell clones cross-reactive against nontyphoidal Salmonella . (nature.com)

The GlmS(6Ala) protein and the CBD-tagged proteins are functional in vivo according to assays conducted at New England Biolabs. (neb.com)
Meanwhile, it was determined that treatment with autophagy inhibitor 3-methyladenine and angiotensin II type 1 receptor (AT1R) blocker telmisartan could improve insulin secretion and apoptosis in vitro and in vivo. (bvsalud.org)

The IL-1 receptor type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail and has no signaling function. (rndsystems.com)
Structurally, all known receptor PTPases, are made up of a variable length extracellular domain, followed by a transmembrane region and a C-terminal catalytic cytoplasmic domain. (embl.de)

Figure 5: Involvement of cooperating transcription factors at estrogen receptor binding sites. (nature.com)

A helper cells are primarily responsible for helping other vaccine approach based on cell-mediated immunity that immune cells through direct cell-cell interactions or by avoids some of these drawbacks is discussed here. (cdc.gov)

We undertook this study to investigate receptor (IL-2R) accumulation is related to whether the cytokine accumulation in PCs the leukocyte content of the platelet comc obtained from single apheresis donors was ponent and can be reduced by prestorage beyond the acceptable limits of WBC conc leukocyte depletion [ 3 ]. (who.int)
The alpha granules contain hemostatic proteins such as fibrinogen, vWf, and growth factors (eg, platelet-derived growth factor and transforming growth factors). (medscape.com)

Furthermore, A. fumigatus particles exhibited greater persistence in the lungs of recipient mice when compared to non-viable A. fumigatus or A. versicolor , and this correlated with increased maintenance of airway memory-phenotype CD8 + T cells. (cdc.gov)

Note the image below, as well as the article Pediatric Complement Receptor Deficiency may be helpful. (medscape.com)

Overexpression of Pex15p, a phosphorylated peroxisomal integral membrane protein required for peroxisome assembly in S. cerevisiae , causes proliferation of the endoplasmic reticulum membrane. (microbiologyresearch.org)

The multiligand receptors that form the focus of this Perspective series have expectedly diverse functions, often conforming to potential gaps in the host response to invading pathogens that are not effectively manned by adaptive immunity. (jci.org)
Greater understanding of how each subset contributes to V protective immunity and how T-cell memory is maintained accine approaches against respiratory virus infections and recalled in a secondary infection would contribute to such as influenza have relied on inducing antibodies development of effective vaccines that use these basic fea- that protect against viral infection by neutralizing virions tures of the immune response. (cdc.gov)

We have purified and cloned a HeLa cell nuclear protein that strongly stimulates binding of retinoic acid and thyroid hormone receptors (RARs and TRs) to response elements. (nih.gov)
and amphoterin, a nuclear protein sometimes found in the ECM (Table 1 ). (jci.org)
Electrophoretic mobility shift assays detected allele-dependent nuclear protein binding in A549 cells for 8 of 21 variants. (cdc.gov)

ELF5 and DOK7 regulation in anti-estrogen treated cells and tumors. (umass.edu)

If living cells from mouse strain CBA were injected into an adult mouse of strain A, some immunologic process destroyed the CBA cells, and the A-line mouse that received the CBA cells quickly destroyed any subsequent graft from the same donor strain. (medscape.com)

Rohrschneider generated similar antisera and used them to good effect, performing some of the first immunoprecipitation and immunofluorescence experiments to identify the Src protein in Rous sarcoma virus-infected model organisms and to localize the protein to the cytoplasm. (fredhutch.org)
Using improved immunofluorescence microscopy, Rohrschneider soon realized that most of the Src oncoprotein is actually concentrated in small regions of the cell membrane where the internal cytoskeleton sticks to the external matrix that makes up the glue of connective tissue. (fredhutch.org)
Double-labelled immunofluorescence showed that both p33 and p92 replicase proteins localized to peroxisomes, independently of one another and of the presence of the replication template. (microbiologyresearch.org)

An inflammation response that mobilizes both T and B cells results in the creation of antibodies against these cells, causing them to become less responsive to insulin by an as-yet unknown mechanism and promoting hypertension , hypertriglyceridemia , and arteriosclerosis , hallmarks of the metabolic syndrome . (wikidoc.org)

Results showed that existence of AT1-AA impaired the islet function and increased the apoptosis of pancreatic islet cells in rats, and the autophagy level in rat pancreatic islet tissues tended to increase gradually with the prolongation of immunization time. (bvsalud.org)
AT1-AA markedly reduced INS-1 cell viability, promoted cell apoptosis, and decreased insulin secretion in vitro. (bvsalud.org)

Introduction tions of T-cell derived IL-2R are increased in mixed lymphocyte cultures. (who.int)

METHODS: ANA and SMA reactivity of 61 AIH sera and 72 non-alcoholic fatty liver disease controls were separately assessed on tissue sections and HEp-2 cells to compare the diagnostic value at increasing titers. (bvsalud.org)
Immunohistochemistry can be used to determine the presence of CD20 on cells in histological tissue sections. (wikidoc.org)

Further, flow cytometry analysis was performed to characterize anti-S memory B lymphocytes by IgG subclasses among PBMC (peripheral blood mononuclear cells) samples, obtained from 11 individuals post-second dose and third dose administration. (news-medical.net)
Additionally, single-cell RNA sequencing (scRNA-seq) analysis was performed for anti-S B lymphocytes obtained from four donor individuals 210.0 days post-second dose and 10.0 days post-third dose of Comirnaty vaccines. (news-medical.net)
Most T cells in the epithelium and adjacent to the damaged basal keratinocytes are activated CD8 + lymphocytes. (medscape.com)

Type 1 diabetes is an autoimmune disease where your body destroys the cells of the pancreas that secrete insulin. (yaledailynews.com)

Anatomy42

Organisms12

Diseases2

Chemicals and Drugs103

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Phenomena and Processes64

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture2

Information Science4

CDNA7

Genes6

Gene18

Peptides6

Antibody12

Antigen11

Distinct6

Pathway6

Soluble1

Sequences3

Epithelial4

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Expression18

Signal Transduction1

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Molecular2

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Human14

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Surface6

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Humans2

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Activation5

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Proto-oncogene2

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Helper cells1

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