Cell biol hela cells. Medical search. Frequent questions

HeLa cells stably expressing BAC-encoded GFP-labeled snRNP protein Snu114 and imaged witb a Leica SP5 confocal microscope using a 60x 1.4NA HCX Plan Apo objective lens. (cellimagelibrary.org)
HeLa cell lines containing stably integrated copies of the fusion gene were synchronized by double thymidine block. (umassmed.edu)
Chromosome order in HeLa cells changes during mitosis and early G1, but is stably maintained during subsequent interphase stages. (ox.ac.uk)
We conclude that CT arrangements were stably maintained from mid G1 to late G2/early prophase, whereas major changes of CT neighborhoods occurred from one cell cycle to the next. (ox.ac.uk)

To study the poliovirus-cell interaction, eight monoclonal antibodies that specifically block the cytopathic effects of poliovirus were generated by using HeLa cell preparations as immunogen and a newly developed colorimetric screening assay. (ncsu.edu)

Surface functions during mitosis I: phagocytosis, pinocytosis and mobility of surface bound Con A. Cell 15: 327-341. (uconn.edu)

Checkpoint kinase 2 (CHEK2), a cell cycle checkpoint regulator gene, codes for a kinase protein activated in response to radiation and other agents that cause breaks in the DNA. (termedia.pl)
Monoclonal antibody identification of a 100-kDa membrane protein in HeLa cells and human spinal cord involved in poliovirus attachment. (ncsu.edu)
HeLa cells expressing Myc-GnT1IP-L (Myc-tagged GlcNAcT-1 inhibitory protein, long transcript, membrane-anchored) (red) and GlcNAcT-1-HA (green) were treated with cycloheximide and brefeldin A to cause retrograde transport of Golgi enzymes into the ER. (cellimagelibrary.org)
When cells were transfected with GFP-HIF-1α, treatment with TSA increased the number of green fluorescence protein (GFP)-positive cells. (oncotarget.com)
This control is propagated through the alpha-subunit of prolyl 4-hydroxylase where protein levels were previously shown to rise fivefold with increasing cell density. (embl.de)
Using a fluorescent sulfhydryl probe as well as a sulfhydryl inhibitor, one observes a strong cell density response, supporting the hypothesis that cellular redox potential could alter protein stability. (embl.de)
Important biological events associated with plasma membranes, such as signal transduction, cell adhesion, and protein trafficking, are mediated through the membrane microdomains. (mdpi.com)
A major component of the regulation of histone protein synthesis during the cell cycle is the modulation of the half-life of histone mRNA. (umassmed.edu)
The microtubules in cells that make smaller amounts of this protein were more disorganised, and these cells were unable to fuse with each other to form muscle cells. (elifesciences.org)
The subunit alpha protein activates transcription of the subunit beta gene" J. Biol. (proteinkinase.biz)
Chester N, Yu IJ, Marshak DR (1995) "Identification and characterization of protein kinase CKII isoforms in HeLa cells. (proteinkinase.biz)
The glial cells (black dots) and nerve cells (brown bands) in this developing fruit fly nerve cord formed normally despite the absence of the SPITZ protein, which bloc. (ucsd.edu)
HeLa cells were processed for indirect immunofluorescence with antibodies to two nuclear pore components, ELYS (embryonic large protein derived from yolk sac) and mAb414 against the FG nucleoporins. (ucsd.edu)
Exposure of human cell lines, HCT15, PLC/PR/5, HeLa, and Chang, to cadmium chloride resulted in cytotoxicity and cell death, and this was associated with a significant decrease in eIF4E protein levels. (cdc.gov)
Further studies revealed the absence of alterations in the eIF4E mRNA level in the cadmium -treated cells despite their decreased eIF4E protein level. (cdc.gov)
In addition, exposure of cells to cadmium resulted in enhanced ubiquitination of eIF4E protein while inhibitors of proteasome activity reversed the cadmium -induced decrease of eIF4E protein. (cdc.gov)
Taken together, our results demonstrate that the exposure of cells to cadmium chloride resulted in cytotoxicity and cell death due to enhanced ubiquitination and consequent proteolysis of eIF4E protein, which in turn diminished cellular levels of critical genes such as cyclin D1. (cdc.gov)
The capsid-coat protein serves multiple functions, including (1) protecting the viral RNA from degradation by environmental RNase, (2) determining host and tissue tropism by recognition of cell-specific cell-membrane receptors, (3) penetrating target cells and delivering the viral RNA into the cell cytoplasm, and (4) selecting and packaging viral RNA. (medscape.com)

Transfected HeLa cells were fixed in 3% paraformaldehyde, blocked with 0.2% Triton X-100, 1% FBS, 0.5% BSA in PBS with Ca[2+] and Mg[2+], followed by incubation in primary antibodies (anti-Myc mouse mAb, 9E10 (Covance) and anti-HA mouse mAb, HA.11 (Covance)) and secondary antibodies (Alexa 568 and Alexa 488) and DAPI (blue) to stain nuclei. (cellimagelibrary.org)
To analyze possible changes of chromosome arrangements from one cell cycle to the next, nuclei were photobleached in G2 maintaining a contiguous zone of unbleached chromatin at one nuclear pole. (ox.ac.uk)
Accordingly, chromatin patterns observed in daughter nuclei differed significantly from the mother cell nucleus. (ox.ac.uk)

For HeLa cell nucleus volume of 690 um^3 see BNID 104716 . (harvard.edu)
Similarly, the large ribosomal RNA gene clusters that reside on different chromosomes manage to find each other in almost every cell nucleus to form another easily discernable nuclear entity, the nucleolus [ 27 - 29 ]. (biomedcentral.com)
The nuclear lamina, which coats the inner nuclear cell membrane, represents a major repressive environment in the nucleus. (biomedcentral.com)

Nup133 (a component of the Nup107-160 nucleoporin complex, red) localizes to spindle poles and kinetochores while NuMA localizes to spindle poles (green) in prometaphse Hela cells. (ucsd.edu)

Furthermore, AF3 detected the band in a human-mouse hybrid cell line containing human chromosome 19, which confers permissivity for poliovirus infection, but the antibody did not detect the band in a human chromosome 19-deficient subclone. (ncsu.edu)

T. Twellmann, T.W. Nattkemper, W. Schubert, H. Ritter, Cell detection in micrographs of tissue sections using support vector machines, in: Proceedings of the ICANN: workshop on Kernel & Subspace Methods for Computer Vision, Vienna, Austria, 2001, pp. 79–88. (crossref.org)

It is also involved in cell cycle arrest and apoptosis [17]. (termedia.pl)
Mishra RR, Chaudhary JK, Rath PC (2012) Cell cycle arrest and apoptosis by expression of a novel TPIP (TPIP-C2) cDNA encoding a C2-domain in HEK-293 cells. (jnu.ac.in)

N 6 -methyladenosine modification destabilizes developmental regulators in embryonic stem cells. (nature.com)
Examples of a novel series of phenanthrolinones are shown to be potent competitive inhibitors of avian prolyl 4-hydroxylase, and of collagen hydroxylation, in embryonic chick tendon cells and human foreskin fibroblasts in vitro and in the oestradiol-stimulated rat uterus in vivo. (embl.de)
In embryonic avian tendon, cell density regulates collagen production. (embl.de)

Whether chromosomes maintain their nuclear positions during interphase and from one cell cycle to the next has been controversially discussed. (ox.ac.uk)

Maul GG, Deaven L. Quantitative determination of nuclear pore complexes in cycling cells with differing DNA content. (harvard.edu)
Rath P.C. (2005) Relationship between constitutive Nuclear Factor-kappaB (NF-kB) and Inhibitor kappaB-alpha (IkB-a) in an Interferon-a-sensitive human Burkitt lymphoma cell line. (jnu.ac.in)

In anti-metastatic studies, lycopene has been found to inhibit the metastasis of human hepatoma SK-Hep-1 cells in athymic nude mice ( 17 ). (frontiersin.org)
Moreover, we recently reported that lycopene inhibits the metastasis of human liver adenocarcinoma SK-Hep-1 cells by downregulation of the NADPH oxidase 4 (NOX4) proteins ( 18 ). (frontiersin.org)
To this topic, lycopene had been demonstrated to have synergism from the combination of oxaliplatin in a human ovarian cancer cell line ( 20 ), suggesting that the adjuvant property on the anticancer of lycopene. (frontiersin.org)
Furthermore, AF3 reacted with human peripheral mononuclear cells, consistent with the known replication of poliovirus in Peyer's patches and tonsils. (ncsu.edu)
Mishra RR, Chaudhary JK, Bajaj GD, Rath PC (2011) A novel human TPIP splice-variant (TPIP-C2) mRNA, expressed in human and mouse tissues, strongly inhibits cell growth in HeLa cells. (jnu.ac.in)
Rath P.C. and Mukhopadhyaya T. (2009) p53 gene expression and 2-methoxyestradiol treatment differentially induce NF-kB activation in human lung cancer cells with different p53 phenotypes, DNA Cell Biol. (jnu.ac.in)
Moreover, knockdown of METTL3 significantly affected angiogenesis of the human endothelial cells. (biomedcentral.com)
Whether translation initiation factor 4E (eIF4E), the mRNA cap binding and rate-limiting factor required for translation, is a target for cytotoxicity and cell death induced by cadmium , a human carcinogen, was investigated. (cdc.gov)

In Fall 2012 I taught Cell Biology and a community-based research senior seminar in Health Studies. (dickinson.edu)
Appreciate how our understanding of cell biology has resulted in medical advances, and discuss current biomedical issues in a cellular context. (dickinson.edu)

During the metastasis, cancer cells in situ move to other tissues and organs from the blood circulation or the lymphatic system through proliferation, adhesion, invasion, and migration. (frontiersin.org)
Plaque-inhibition assays confirmed the viral specificity of the antibodies, and when one antibody, AF3, was used as a probe in immunoblots of cell membrane preparations, it detected a 100-kDa band in only those cell lines and tissues permissive for poliovirus infection. (ncsu.edu)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
This is due to acute alterations in the tissues surrounding the tumor and in the environment as a result of treatment with anticancer therapeutics, which facilitate the drug resistance of tumor cells. (oncotarget.com)

In mammalian cells, most of the redox potential used for generating ATP arrives at the mitochondrion in the form of the nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FADH 2 ), reduced coenzymes generated by the acceptance of electrons derived from the breakdown of organic substances in the tricarboxylic acid (TCA) cycle. (biomedcentral.com)

With the exception of the xylem, where there are no transverse cell divisions, each file of cells commences with its initial cell and then possesses a zone of concomitant cell expansion and transverse cell division, followed, after completion of the divisions, by a zone of terminal cell differentiation. (biologists.com)
Three types of behaviour, all related to changes in the cell walls, were noted as cortex, xylem and sieve element cells entered their respective phases of cell differentiation. (biologists.com)
The microtubule cytoskeleton is critical for muscle cell differentiation and undergoes reorganisation into an array of paraxial microtubules, which serves as template for contractile sarcomere formation. (elifesciences.org)
We show that oMAP4 is expressed upon muscle cell differentiation and is the only MAP4 isoform essential for normal progression of the myogenic differentiation programme. (elifesciences.org)
Upon differentiation, mono-nucleated myoblasts withdraw from the cell cycle and fuse to form syncytial myotubes ( Wakelam, 1985 ). (elifesciences.org)

Depletion of oMAP4 impairs cell elongation and cell-cell fusion. (elifesciences.org)

It has long been considered to play a role in cell growth and proliferation and considerable evidence suggests that it can also exert potent suppression of apoptosis in cells. (proteinkinase.biz)
Cancer cells share six features that distinguish them from normal cells: Autocrine production of growth signals, inability to respond to anti-growth signals, sustained angiogenesis, limitless replicative potential, tissue invasion and metastasis, and apoptosis avoidance [ 1 ]. (biomedcentral.com)
Tumor cells are subjected to stressful internal and external environments, but nevertheless are resistant to apoptosis. (biomedcentral.com)

In addition, Methylated RNA Immunoprecipitation Sequencing technology (MeRIP-seq) was performed to screen downstream targets of METTL3 in endothelial cells and to fully clarify the specific underlying molecular mechanisms affecting the phenotype of cerebral AVM. (biomedcentral.com)
Ultimately, the up-regulated downstream genes of Notch signaling pathway dramatically affected the angiogenesis of endothelial cells. (biomedcentral.com)
In addition, we demonstrated that blocking Notch pathway with DAPT could restore the phenotype of METTL3 deficient endothelial cells. (biomedcentral.com)

Immunocytochemical labeling of phosphorylated MLC in paraformaldehyde fixed A7r5 cells. (ecmbio.com)

Cell density regulates prolyl 4-hydroxylase activity independent of mRNA levels. (embl.de)
Distinct populations of H3 histone mRNA were produced by heat induction in early S-phase, late S-phase, or G2-phase cells, and the stability of the induced H3 histone mRNA was measured. (umassmed.edu)
The H3 histone mRNA induced in non-S-phase cells is more stable than that induced in late S phase, with a half-life of 40 min. (umassmed.edu)
Thus, the stability of histone mRNA is actively regulated throughout the cell cycle. (umassmed.edu)

These proteins are located on the plasma membrane of cells throughout the body. (guidetopharmacology.org)
caspases (e.g. caspase-3, -6, and -7) in a cascade-like manner, which cleave key cellular proteins that lead to the morphological changes associated with apoptotic cell death. (enzolifesciences.com)

To address this question, we performed long-term live-cell studies using a HeLa cell line with GFP-tagged chromatin. (ox.ac.uk)

These inhibitors inhibit NHEJ-catalyzed DSB repair and sensitize in vitro cancer cells to DSB-inducing agents. (termedia.pl)

Unique receptor sites for poliovirus are considered to be the primary determinant of the virus' cell and tissue-type specificity. (ncsu.edu)
Primary source (p.2251 left column 2nd paragraph) gives range of 3,000 to 5,000 molecules/cell. (harvard.edu)
Primary source studied HeLa cells. (harvard.edu)
Among immunodeficient individuals, toxoplasmosis most often occurs in those with defects of T-cell-mediated immunity, such as those with hematologic malignancies, bone marrow and solid organ transplants, or acquired immunodeficiency syndrome ( AIDS ).In most immunocompetent individuals, primary or chronic (latent) T gondii infection is asymptomatic. (medscape.com)

For example, microarray technology has been used to compare gene expression profiles between normal and diseased cells and this has led to dramatic advances in the understanding of cellular processes at the molecular level [ 1 ]. (biomedcentral.com)
Exposure of cells to cadmium , as well as the specific silencing of eIF4E gene, also resulted in decreased cellular levels of cyclin D1, a critical cell cycle and growth regulating gene, suggesting that the observed inhibition of cyclin D1 gene expression in the cadmium -treated cells is most likely due to decreased cellular level of eIF4E. (cdc.gov)

The metastasizing cells penetrate the blood vessel wall by invasiveness, and migrate in the circulation, extravasate out of the blood vessels, colonize in the distant tissue, initiate angiogenesis, and finally grow at the new site ( 2 , 3 ). (frontiersin.org)
This type of cell death is fundamental for the maintenance of tissue homeostasis and immune system development [ 2 ]. (biomedcentral.com)

Alternative sites for cell density regulation of the enzyme could rely on its sensitivity to sulfhydryl groups. (embl.de)

Cell surface distribution of lectin receptors determined by resonance energy transfer. (uconn.edu)

We found that cells exhibit a broad range of nonstoichiometric m 6 A levels with cell-type specificity. (nature.com)

were prepared for electron microscopy so that cortical microtubules could be counted along the longitudinal walls in cell files in the endodermis, pericycle, and inner and outer cortex, and in sieve and xylem elements. (biologists.com)
The cells augment their population of cortical microtubules as they elongate and divide, showing a net increase of up to 0.6 μ m of polymerized microtubule length per min. (biologists.com)
This initial quota becomes diluted as the second sub-process commences, (ii) The cells interpolate new microtubules at a rate which is characteristic of the cell, and, in the endodermis, of the face of the cell, while the cell elongates. (biologists.com)
Most cell types thus maintain a set density of cortical microtubules while they elongate and divide. (biologists.com)
Comparisons of endodermal cells in untreated controls, and roots that had been treated with colchicine, low temperature, or high pressure indicate that the initial quota of microtubules, and the later interpolations, and differentially sensitive to microtubule perturbations. (biologists.com)
The cortical cells expanded in all dimensions, and the interpolation of microtubules diminished or ceased. (biologists.com)
The sieve elements continued to elongate, and interpolated at a high rate, reaching unusually high densities of microtubules when the cell walls were being thickened. (biologists.com)
Therefore, the next challenge is to understand how other processes-such as the selective stabilisation of some microtubules and the movement of cell materials along the microtubules-cooperate to control muscle fibre formation. (elifesciences.org)

T. Kämpfe, T.W. Nattkemper, H. Ritter, Combining independent component analysis and self-organizing maps for cell image classification, Proc. (crossref.org)

The methylation pattern within a specific multigene locus is influenced by the number and type of rRNA genes in other rDNA loci in the cell. (ncsu.edu)

Cells called myoblasts elongate and fuse together at their tips to make the muscle cells. (elifesciences.org)

Indeed, cells tightly regulate m 6 A modification using specialized enzymes, m 6 A writers and erasers. (molcells.org)
In normal cells, the level of CK2 appears to be tightly regulated, and cells resist a change in their intrinsic level of CK2. (proteinkinase.biz)

Earlier reports indicated that underhydroxylated collagen, accumulating within the endoplasmic reticulum in cells where prolyl 4-hydroxylase is inactivated, is slowly degraded, but is then rapidly hydroxylated and secreted when the activity of prolyl 4-hydroxylase is restored. (embl.de)

The genome of the geminivirus tomato golden mosaic virus (TGMV) consists of two circular DNA molecules designated as components A and B. The A component contains the only virally-encoded function required for autonomous replication in infected plant cells. (ncsu.edu)

CK2 has a number of physiological targets and participates in a complex series of cellular functions including the maintenance of cell viability. (proteinkinase.biz)

DNA repair targeted therapy, which kills cancer cells preventing DNA repair [1-3]. (termedia.pl)

Skeletal muscle fibre formation requires a coordinated programme of morphological and biochemical changes in the differentiating cells. (elifesciences.org)

These cells contain stacks of structures called sarcomeres that enable the cells to contract and generate the force required for movement. (elifesciences.org)

Distribution of Nup133 (a component of the Nup107-160 nucleoporin complex, red) and CENP-B (kinetochores, green) in dividing Hela cells. (ucsd.edu)

Isoform-specific differences in rates of assembly from catalytic and regulatory subunits" J. Biol. (proteinkinase.biz)

Transcription of this gene can be switched on and off by raising and lowering cell culture temperatures, respectively. (umassmed.edu)

Anatomy40

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Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Phenomena and Processes56

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