AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesAnthropology, Education, Sociology and Social PhenomenaInformation ScienceNamed GroupsHealth Care
Peripheral Blood Stem Cell TransplantationTransplantation, AutologousHematopoietic Stem Cell TransplantationHematopoietic Stem Cell MobilizationTransplantation ConditioningTransplantation, HomologousMelphalanGranulocyte Colony-Stimulating FactorLeukapheresisAntigens, CD34Combined Modality TherapyHematologic NeoplasmsBlood Component RemovalStem Cell TransplantationMultiple MyelomaCyclophosphamideLeukocytes, MononuclearAntineoplastic Combined Chemotherapy ProtocolsMyeloablative AgonistsGraft vs Host DiseaseBusulfanTreatment OutcomeLymphoma, Non-HodgkinBone Marrow TransplantationGraft SurvivalHematopoietic Stem CellsEtoposideThiotepaAntineoplastic Agents, AlkylatingRemission InductionRecurrenceCytophagocytosisCarmustineIfosfamideSalvage TherapyVincristineAmyloidosisStem CellsHematopoiesisCytarabineHodgkin DiseaseCord Blood Stem Cell TransplantationDisease-Free SurvivalTransplantation ChimeraNeoplasmsRetrospective StudiesTissue DonorsTime FactorsSurvival RateWhole-Body IrradiationInfectionLymphoma, T-CellSurvival AnalysisDoxorubicinHistocompatibilityPlatelet CountPOEMS SyndromeImmunosuppressive AgentsHistocompatibility TestingT-LymphocytesRecombinant ProteinsBlood CellsPrednisoneCarboplatinLeukemia, Myeloid, AcuteDexamethasoneBlood Cell CountTransplantation, IsogeneicLeukemiaVidarabineFollow-Up StudiesAntineoplastic AgentsCells, CulturedLymphomaImmunophenotypingCytomegalovirus InfectionsFlow CytometryProspective StudiesBone Marrow PurgingFatal OutcomeGraft vs Leukemia EffectFeasibility StudiesLymphocytesLymphocyte ActivationAnemia, AplasticMyelodysplastic SyndromesAntigens, CDCell SeparationLymphocyte DepletionBone MarrowLeukemia, Myelogenous, Chronic, BCR-ABL PositiveLiver TransplantationBlood Transfusion, AutologousHLA AntigensBlood DonorsLeukocyte CountMonocytesCyclosporineImmunosuppressionPrognosis
Allogeneic stem cell transEmbryonic stemLymphocytesMethodsImmunotherapyCytokinesHematopoetic stemAdultCurative treatment for acuteTumor cellsDifferentiationDifferentiateStromal cellEngraftmentVitroPatientsPBMCMSCsLiver transplantationBone marrow derivedEndothelial cellsAdipose-derivedTherapeuticsDonorsPluripotentLineagesVivoHSCTTherapy in retinalTissuesUmbilicalNonmyeloablativeDonorTherapiesRenal cell carcGenetically modified2016Progenitor cell therapyChemotherapySignaling pathwaySubpopulationsDiseasesAntigen-specificRegenerationHumanClinical studiesAnti-inflammatoryMultiple myelomaSurvivalCellularCord bloodInnateImmune cells
Allogeneic stem cell trans4
Embryonic stem9
Lymphocytes7
  • 3 Autologous lymphocytes genetically modified with the TCR for MART-1/Melan-A have been adoptively transferred to patients with advanced melanoma. (haematologica.org)
  • Although human CD20 has previously been described as functional selection marker and suicide gene after retroviral transfer to T lymphocytes, 9 , 10 the specificities of these T cells were unknown, and therefore a potential effect of CD20 on antigen-specific T-cell functions could not be investigated. (haematologica.org)
  • CD8+ T cells, also called cytotoxic T lymphocytes (CTL), are one subtype of T cell ( 6 ), characterized by robust production of interferon (IFN)-γ and cytolytic activities via perforin (PRF)/granzymes (GZM) or Fas mechanisms to kill target cells ( 7 ). (frontiersin.org)
  • Recent studies have shown that soluble factors elaborated by human T lymphocytes enhance erythroid burst formation by human peripheral blood null cells. (shengsci.com)
  • A slow clearance of leukemic cells by the initial course of chemotherapy, as measured by the morphologic analysis of peripheral blood or bone marrow smears 1 to 3 weeks from diagnosis, predicts a higher risk of relapse, but can be inaccurate because ALL blast cells are remarkably similar in appearance to bone marrow lymphoid precursors (alias hematogones) and, in some instances, to mature lymphocytes. (ashpublications.org)
  • The first demonstration that bone marrow samples apparently devoid of ALL cells may, in fact, contain measurable levels of leukemia (ie, "minimal residual disease" or MRD) was provided soon after the advent of polyclonal antisera against leukocyte antigens: combinations of cell markers were used to distinguish T-lineage ALL cells from normal immature and mature lymphocytes by immunofluorescence microscopy. (ashpublications.org)
  • This was associated with increased infiltration of macrophages, lymphocytes, and mast cells. (oaji.net)
Methods3
  • METHODS: ABL602 candidates were compared in terms of binding activity, T-cell activation, and tumor-killing activities. (bvsalud.org)
  • Through the incorporation of novel computational methods for predicting antigen changes to model-informed drug discovery and development, superior vaccines and antibody-based therapeutics or diagnostics can now be more easily designed to mitigate failures. (preprints.org)
  • Therefore, novel therapeutic methods for patients with ALF are urgently required. (springer.com)
Immunotherapy1
Cytokines9
  • ABL602-mediated T-cell activation and tumor-killing activities were determined by measuring the expression of activation markers, cytokines, cytolytic proteins, and the proportion of dead cells. (bvsalud.org)
  • Compared with the reference 1+1 antibody, ABL602 did not induce the release of cytokines including interleukin-6 and tumor necrosis factor-α in the healthy donor-derived peripheral blood mononuclear cell. (bvsalud.org)
  • Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
  • The activation, differentiation, and development of CD8+ T cells can be affected by numerous inflammatory cytokines, transcription factors, and chemokines. (frontiersin.org)
  • Various cytokines, transcription factors, and other regulatory molecules have been found to be differentially methylated in CD8+ T cells in autoimmune diseases. (frontiersin.org)
  • The activation, differentiation, and development of CD8+ T cells is accompanied by large-scale changes in the coordinated expression of numerous inflammatory cytokines, transcription factors (TFs) and chemokines that are correlated with their survival, effector function, and self-renewal ( 7 ). (frontiersin.org)
  • We also present the data of our own research concerning evaluation of the prognostic role of cytokines and lymphocyte subpopulations in peripheral blood of the TNBC patients. (cttjournal.com)
  • Inhibits proinflammatory cytokine secretion and increases anti-inflammatory cytokines from peripheral blood mononuclear cells. (medscape.com)
  • The presence of inflammatory cytokines after high-dose chemotherapy leads to proliferation and activation of MDSCs originating from autologous hematopoietic progenitors at the time of engraftment. (biomedcentral.com)
Hematopoetic stem1
  • The bone morrow contains several stem cell types including hematopoetic stem cells, endothelial progenitor cells, mesenchymal (stromal) stem cells and multipotent adult progenitor cells. (escardio.org)
Adult11
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • Adult stem cells are found in a few select locations in the body, known as niches, such as those in the bone marrow or gonads. (wikipedia.org)
  • Several investigations [ 5 - 7 ] have been carried out with isolated embryonic, fetal, and adult SCs in a well-defined culture microenvironment to define the sequential steps and intracellular pathways that are involved in their differentiation into the specific cell lineages. (biomedcentral.com)
  • A blastocyst contains an inner cell mass including a clump of around 150 cells that eventually will generate the entire body of the adult animal. (bmrat.org)
  • This way of making iPSCs from adult cells circumvents the need to destroy an embryo from living donor. (bmrat.org)
  • Adult stem cells were firstly isolated from bone marrow in mice (Spangrude, Heimfeld, and Weissman, 1988) and later in humans. (bmrat.org)
  • Sadan O, Shemesh N, Cohen Y, Melamed E, Offen D. Adult neurotrophic factor-secreting stem cells: a potential novel therapy for neurodegenerative diseases. (jamanetwork.com)
  • In vascular medicine, various stem cells and adult progenitors have been highlighted as having a vasoreparative role in ischaemic tissues. (biomedcentral.com)
  • More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)
  • If members of the family do not match, the next options are to seek a match from the registries of unrelated adult donors, or from a registry of donated cord blood. (biohellenika.gr)
  • Mesenchymal stem cells (MSCs) are multipotent adult stem (or stromal) cells with the capacity of self-renewal and differentiation potential towards several mesodermal linages such as osteogenic, chondrogenic and adipogenic differentiation (more citations) [ 1 ]. (biomedcentral.com)
Curative treatment for acute2
Tumor cells7
  • Clones from three patients with a partial response or stable disease recognized antigens expressed on renal cell carcinoma tumor cells. (aacrjournals.org)
  • CD8 + CTL-recognizing minor H antigens on tumor cells can be isolated posttransplant and could contribute to the graft- versus -tumor effect. (aacrjournals.org)
  • The aim of adoptive T-cell therapy of cancer is to selectively confer immunity against tumor cells. (haematologica.org)
  • ALCL was recognized in 1985, when tumor cells consistently demonstrated labeling by the monoclonal antibody Ki-1, a marker later shown to recognize the CD30 antigen. (medscape.com)
  • Unlike primary NK cells, those prepared from iPSCs can be prepared with a homogeneous quality and are easily modified to exert a desired response to tumor cells. (molcells.org)
  • Furthermore, T cells and NK cells complement each other in that certain immune suppression mechanisms taken by tumor cells that are effective against T cells, such as the downregulation of human leukocyte antigens (HLA), have proven to be stimulatory for NK cells. (molcells.org)
  • Results ADCC and ADCP assays showed that particularly neutrophils and macrophages from healthy donors, non-human primates and patients with neuroblastoma are able to kill neuroblastoma tumor cells efficiently with IgA3.0 ch14.18. (bmj.com)
Differentiation7
  • Moreover, it seems that paracrine action of skeletal myoblasts facilitate neighboring cardiomyocytes to maintain their replicative potential and/or stimulate differentiation of native cardiac stem cells (8,9). (escardio.org)
  • Mesenchymal stem cells (MSCs) have been widely tested for treating a variety of refectory medical indications such as type 1 diabetes mellitus, systemic lupus erythematosus, rheumatoid arthritis and Crohn's disease due to their multiple differentiation potentials and immunomodulation capability [1-5]. (researchsquare.com)
  • To determine therapeutic potential of hematopoietic stem cell (HSC), we need to understand better correlation between the metabolic/energy status of the cells and stages of stem cell differentiation during hematopoiesis. (gov.rs)
  • Accordingly, scientists have developed protocols for the expansion, genetic modification and differentiation of stem cells to NK cells. (molcells.org)
  • In isolated naive CD4 + T-cells from HD and patients, MSCs suppressed the differentiation of naive towards an effector phenotype while memory and naive cells showed higher percentages in culture with MSCs. (biomedcentral.com)
  • The results of the study indicate significant immunomodulatory properties of MSCs, as under Th17-polarizing conditions MSCs are still able to control T-cell differentiation and proinflammatory cytokine production in both HD and patients with autoimmune arthritis. (biomedcentral.com)
  • In the presence of TGF-β, IL-9 may induce differentiation of naive CD4 + T-cells towards Th17 cells, while IL-9 also affects thymus-derived natural Tregs (nTregs) and enhances their suppressive function in vitro [ 26 ]. (biomedcentral.com)
Differentiate12
  • In multicellular organisms, stem cells are undifferentiated or partially differentiated cells that can differentiate into various types of cells and proliferate indefinitely to produce more of the same stem cell. (wikipedia.org)
  • In vivo, they eventually differentiate into all of the body's cell types (making them pluripotent). (wikipedia.org)
  • They exist to replenish rapidly lost cell types and are multipotent or unipotent, meaning they only differentiate into a few cell types or one type of cell. (wikipedia.org)
  • they are vastly outnumbered by the progenitor cells and terminally differentiated cells that they differentiate into. (wikipedia.org)
  • Hematopoietic stem cells (HSCs) were first identified in 1961, and their ability to migrate and differentiate into multiple cell types was documented [ 2 ]. (biomedcentral.com)
  • A progenitor cell is a biological cell that, like an SC, has a tendency to differentiate into a specific type of cell but is already more specific than an SC and is pushed to differentiate into its 'target' cell. (biomedcentral.com)
  • MSCs have the capacity of multiple differentiations and self-renewal and have been proven to be able to differentiate into a series of cell lineages, such as adipocytes, osteoblasts, chondroblasts, and hepatocyte-like cells (HLCs). (springer.com)
  • Our bodies contain a pool of stem cells that have the ability to differentiate into any other cell type in the body. (bmrat.org)
  • Stem cells are widely defined by two main characteristics: the ability to self-renew (divide in a way that reproduces more identical stem cells) and to differentiate (to turn stem cells into specialized cells that form different organs and tissues). (bmrat.org)
  • There already exist several protocols to genetically modify and differentiate iPSCs into NK cells, and each has its own advantages with regards to immunotherapies. (molcells.org)
  • Later, Arnold Caplan's group described mesenchymal stem/stromal cells (MSCs) as multipotent mesenchymal cell populations which can differentiate into several tissue types, and demonstrated roles for MSCs in the regeneration of bone, cartilage or ligaments in animal and clinical studies [2-4]. (stemcellslab.net)
  • This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
Stromal cell3
  • When administered post-stroke, mesenchymal stromal cell-derived extracellular vesicles (MSC-EVs) induce neuroprotection, promote brain remodeling and plasticity, and enhance neurological recovery in rodents and non-human primates via mechanisms that involve immunomodulation and anti-inflammation. (explorationpub.com)
  • Recently, mesenchymal stromal cell (MSC)-derived small extracellular vesicles (EVs) have emerged as a potential new therapeutic agent for stroke treatment. (explorationpub.com)
  • Human mesenchymal stromal cell transplantation modulates neuroinflammatory milieu in a mouse model of amyotrophic lateral sclerosis. (jamanetwork.com)
Engraftment6
  • Cell therapy is a potential new treatment for post MI patients, but the main challenges are : the necessity to preserve immune competency and to gain adequate nutrition and homing signals necessary for stem cells' engraftment and survival. (escardio.org)
  • Repair of scar tissue constitutes a challenge for cardiac stem cell therapy due to lack of adequate nutrition and homing signals necessary for stem cells' engraftment and survival. (escardio.org)
  • Patients were monitored for donor engraftment of myeloid and lymphoid cells, for clinical response by serial imaging, and for immunologic response by in vitro isolation of donor-derived CD8 + CTLs recognizing recipient minor histocompatibility (H) antigens. (aacrjournals.org)
  • Prior to infusion of the CAR-modified immune cells, lymphodepletion is performed in most therapeutic settings to allow efficient cell engraftment [ 10 ]. (nature.com)
  • Long-term engraftment of allogeneic cells necessitates eluding immune-mediated rejection, which is currently achieved by matching for human leukocyte antigen (HLA) expression, immunosuppression, and/or delivery of donor-derived cells to sanctuary sites. (ashpublications.org)
  • Engraftment, clinical, and molecular follow-up of patients with multiple myeloma who were reinfused with highly purified CD34+ cells to support single or tandem high-dose chemotherapy. (shengsci.com)
Vitro5
  • The method of stimulating isolated cells with CSE in vitro has been explored and frequently applied to determine the direct causes in the relationships between cigarette smoking and cellular functions [ 15 ]. (hindawi.com)
  • In this study, in attempt to provide fresh information about the impact of CS on proliferation of EPCs to elucidate the pathophysiological mechanisms of the diseases related to CS in cellular level, we assessed the proliferation of EPCs after interfering the cells with a series of concentrations of CSE for various times of exposure in vitro . (hindawi.com)
  • T-cell receptor (TCR) gene transfer is an attractive strategy for rapid in vitro generation of high numbers of antigen specific T cells. (haematologica.org)
  • Although liver failure can be treated via hepatocyte transplantation, it also faces multiple problems comprising the shortage of high-quality hepatocytes sources, rejection of allogeneic transplants, difficulty to expand, and losing hepatic characteristics in vitro [ 7 , 8 ]. (springer.com)
  • Both MDSC phenotypes pre-ASCT but not post-ASCT similarly suppressed in vitro autologous T and natural killer T cell proliferation. (biomedcentral.com)
Patients32
  • As such, stem cell therapy cannot be regarded as a valid therapeutic option for patients with cardiovascular disease in the present era of evidenced-based medicine, yet it holds great hope for the future. (escardio.org)
  • However, there are still questions waiting for answers and results of large-scale randomized trials are needed for the appearance of myocardial cellular therapy as a clinical therapeutic option for patients with ischemic heart disease. (escardio.org)
  • Cell therapy is currently emerging as a potential new treatment for post MI patients with the assumption that recolonization of the areas of scarred myocardium with exogenously supplied surrogates or precursors of cardiomyocytes can restore function and ultimately affect clinical outcomes. (escardio.org)
  • Several preliminary reports have demonstrated that local stem cell transplantation in patients with acute myocardial infarction is safe and may lead to improved myocardial function and perfusion. (escardio.org)
  • MSCs, if considered as a CTP in therapeutic use, have a huge challenge to achieve stable and uniform biological characteristics for ensuring safety and effectiveness in patients received MSCs treatment. (researchsquare.com)
  • Eight patients received conditioning with fludarabine and low-dose total body irradiation followed by hematopoietic cell transplantation from an HLA-matched sibling donor. (aacrjournals.org)
  • Treatment of metastatic renal cell carcinoma with allogeneic hematopoietic cell transplantation after nonmyeloablative conditioning with fludarabine/total body irradiation is feasible and may induce tumor regression or stabilization in some patients. (aacrjournals.org)
  • Between May 2005 and February 2010, 126 consecutive patients underwent intramyocardial cell injection. (tci-thaijo.org)
  • For a long time, stem cells have been utilized to renew the immune system for radiation or chemo- therapy treated patients. (bmrat.org)
  • PBMCs were separately isolated from the human peripheral blood of patients with AR and normal person (NP). (frontiersin.org)
  • However, we still cannot predict with certainty a rate of recovery in the patients with the same disease undergoing identical therapeutic protocols. (gov.rs)
  • Main goal of this work is to investigate metabolic status of peripheral blood mononuclear cells (PBMC) in patients with solid tumors (neuroblastoma, Ewing sarcoma) and lymphoma (Hodgkin and nonHodgkin). (gov.rs)
  • Experiments were performed on PBMC samples of patients that were: a) subjected to mobilization of stem cells from bone marrow, b) not subjected to mobilization, and c) healthy donors (control group). (gov.rs)
  • Between February 1986 and March 1990, 56 patients with relapsed Hodgkin's disease treated with high-dose cyclophosphamide, carmustine, and etoposide (CBV) received an autologous peripheral stem cell transplantation (PSCT) rather than an autologous bone marrow transplantation (ABMT) because each patient had a marrow abnormality, either hypocellularity or tumor involvement. (shengsci.com)
  • Eighty-two patients with advanced multiple myeloma (MM) were enrolled in 2 sequential clinical studies of 1 or 2 courses of myeloablative therapy with stem cell support. (shengsci.com)
  • Absolute values of immunoglobulin free light chains are prognostic in patients with primary systemic amyloidosis undergoing peripheral blood stem cell transplantation. (medscape.com)
  • For individuals who have acute cardiac ischemia who receive progenitor cell therapy, the evidence includes 2 randomized controlled trials (RCTs) with 200 patients, numerous small RCTs, and meta-analyses of these RCTs. (southcarolinablues.com)
  • Additional larger trials are needed to determine whether progenitor cell therapy improves health outcomes in patients with refractory angina. (southcarolinablues.com)
  • Identification of new immunodominant epitopes will facilitate and broaden immune assessment strategies to identify patients suitable for T-cell transfer. (biomedcentral.com)
  • Human adenovirus (HAdV) infection constitutes a major cause of morbidity and mortality in patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT). (biomedcentral.com)
  • The public clinical trials database http://clinicaltrials.gov shows 123 clinical trials using MSCs for a very wide range of therapeutic applications (Figure 1 ), the majority of which are in Phase I (safety studies), Phase II (proof of concept for efficacy in human patients), or a mixture of PhaseI/II studies (Figure 2 ). (biomedcentral.com)
  • Emergence of exhausted B cells in asymptomatic HIV-1-infected patients naïve for HAART is related to reduced immune surveillance. (prelekara.sk)
  • Gross elevation of TT virus genome load in the peripheral blood mononuclear cells of cancer patients. (prelekara.sk)
  • Cerebral Palsy Expanded Access Program in Europe November 2019 Frances Verter, PhD Cerebral Palsy patients and families living in Europe now have expanded access to a program of therapy with cord blood stem cells. (biohellenika.gr)
  • Patients that meet these conditions can receive cord blood stem cell therapy in a hospital in Poland. (biohellenika.gr)
  • The importance of proinflammatory T-cells and their cytokine production in patients with autoimmune arthritis has been widely described. (biomedcentral.com)
  • The aim of this study was to investigate the influence of MSCs on the phenotype, cytokine profile, and functionality of naive and non-naive CD4 + T-cells from healthy donors (HD) and patients with autoimmune arthritis under Th17-cytokine polarizing conditions in an explorative way using a transwell system prohibiting any cell-cell-contact. (biomedcentral.com)
  • In patients, MSCs significantly decreased the proportion of IL-9 and IL-17 producing effector T-cells. (biomedcentral.com)
  • IL-9 and Th9 cells are overexpressed in synovial tissue while proportion of Th9 cells are increased in peripheral blood from RA patients [ 28 ]. (biomedcentral.com)
  • We performed absolute quantification of BAALC copy numbers in peripheral blood prior (median 7 days) to HSCT in complete remission (CR) or CR with incomplete peripheral recovery in 82 acute myeloid leukemia patients using digital droplet PCR (ddPCR) technology. (oncotarget.com)
  • Evaluation of pre-HSCT BAALC / ABL1 copy numbers in peripheral blood by ddPCR represents a feasible and rapid way to identify acute myeloid leukemia patients at high risk of early relapse after HSCT. (oncotarget.com)
  • eg, red blood cells [RBCs], platelets, plasma based on specific gravity) to use for transfusion in different patients. (msdmanuals.com)
PBMC2
  • Peripheral blood mononuclear cells (PBMC) were collected from healthy donors after approval by the LUMC institutional review board and informed consent according to the Declaration of Helsinki. (haematologica.org)
  • T-cell clones were stimulated every 10-14 days with irradiated allogeneic PBMC and phyto-hemagglutinin in IMDM with 5% human serum (HS), 5% FBS and 100 IU/mL IL-2 as previously described. (haematologica.org)
MSCs18
  • The HUCMSCs derived different donors have individual heterogeneity, which potentially lead to distinct therapeutic outcomes in mouse liver fibrosis, indicating we could make use of the donor-variation of MSCs to screen out guaranteed general indicators of MSCs for specific diseases in further stem cell therapy. (researchsquare.com)
  • The heterogeneity of MSCs discussed above hampers the comparison of the therapeutic among different MSC products when applied to clinic use. (researchsquare.com)
  • Over the past few years, MSCs researches have achieved some inspiring results and some of which moved up to clinic period from preclinical phases, resulting in the marketing approval of a few cell-based therapy products (CTPs) by different national regulatory authorities [18]. (researchsquare.com)
  • We established a Good Manufacturing Practice (GMP) grade cell facility to produce clinic-grade human umbilical cord derived MSCs (HUCMSCs) for treating premature ovarian failure (POF) and recurrent uterine adhesion [18, 20]. (researchsquare.com)
  • Although there is a great deal of preclinical and clinical research about MSCs in the treatment of ALF, the exact mechanism underlying the therapeutic role of MSCs remains unclear [ 6 ]. (springer.com)
  • Therefore, MSCs may help resolve issues restricting the application of hepatocyte transplantation. (springer.com)
  • Mesenchymal stem/stromal cells (MSCs) are increasingly used as an intravenously applied cellular therapeutic. (stemcellslab.net)
  • Pivotal studies by the group of Horwitz in children with osteogenesis imperfecta, an inherited enzyme deficiency of collagen synthesis by mesenchymal cells in bone, opened the field for intravenous use of MSCs. (stemcellslab.net)
  • Therefore, the authors concluded that transplantation of isolated healthy allogeneic MSCs might cure the disease. (stemcellslab.net)
  • In a second study [6], these authors showed that autologous, enzyme-deficient MSCs transduced with a copy of the intact gene resulted in normal collagen production in bone cavities. (stemcellslab.net)
  • After 2000, the therapeutic use of MSCs by intravenous administration was explored by a number of studies in animals and also humans. (stemcellslab.net)
  • The tissue source of the MSCs was in most cases not decisive, and cells from various tissue sources were explored. (stemcellslab.net)
  • and signals from the injected cells were found early after administration of the MSCs at the highest frequencies in the lungs, followed by liver and spleen. (stemcellslab.net)
  • There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
  • The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
  • Due to their immunomodulatory properties, mesenchymal stem cells (MSCs) have come into focus as a potential therapeutic concept. (biomedcentral.com)
  • Magnetically isolated naive and non-naive CD4 + T-cells were stimulated under Th17-polarizing proinflammatory cytokine conditions in presence and absence of bone marrow derived mesenchymal stromal cells (MSCs). (biomedcentral.com)
  • A shift from Th1 to Th2 cells demonstrated by an increase in the levels of IL-4 has been observed upon treatment with MSCs [ 8 ]. (biomedcentral.com)
Liver transplantation2
  • The only curative treatment for ALF is liver transplantation, but there are many restrictions on the application of liver transplantation because of financial considerations, a shortage of donor livers, and immunosuppression-related complications [ 3 ]. (springer.com)
  • Torque Teno virus in children who underwent orthotopic liver transplantation: new insights about a common pathogen. (prelekara.sk)
Bone marrow derived5
  • These include bone marrow-derived cells, peripheral blood derived stem cells and skeletal myoblasts (3). (escardio.org)
  • Interestingly, contradictory data exist for transformation of bone marrow derived progenitor cells (BMPC) into new cardiomyocytes and alternative mechanisms as enhanced neovascularization, enhanced scar tissue formation due to augmented inflammatory response and decreased apoptosis have been suggested for the benefical effects of these cells on myocardial function after myocardial infarction (4). (escardio.org)
  • The bone-marrow-derived endothelial progenitor cells (EPCs) provide an alternative source of endothelial cells (ECs) that contributes to neovessel formation in endothelium structure [ 4 , 5 ]. (hindawi.com)
  • Experimental and human studies with intravitreal bone marrow-derived stem cells have begun. (biomedcentral.com)
  • Sadan O, Melamed E, Offen D. Bone-marrow-derived mesenchymal stem cell therapy for neurodegenerative diseases. (jamanetwork.com)
Endothelial cells2
  • These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
  • Toll-like receptor family (TLRs), pattern recognition receptors, is expressed not only on immune cells but also on non-immune cells, including cardiomyocytes, fibroblasts, and vascular endothelial cells. (biomedcentral.com)
Adipose-derived1
  • Systemic treatment with adipose-derived mesenchymal stem cells ameliorates clinical and pathological features in the amyotrophic lateral sclerosis murine model. (jamanetwork.com)
Therapeutics6
  • The target of an antibody plays a significant role in the success of antibody-based therapeutics and diagnostics, and to an extent, that of vaccine development. (preprints.org)
  • Besides modification based on a second-generation CAR, more advanced CAR-immune cell therapeutics are being tested, which utilize precise insertion of genes to circumvent graft-versus-host disease (GvHD) or employ a dual targeting approach and adapter CARs in order to avoid therapy resistance caused by antigen loss. (nature.com)
  • In his early career Dr. Cogle discovered that blood stem cells make blood vessels and used that biology to invent new therapeutics and diagnostics for people with cancer and heart disease. (ufl.edu)
  • Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)
  • We have chosen to concentrate on the emerging therapeutics that broadly involves a wide range of cell types in clinical trials registered on the National Institutes of Health's clinical trials web site. (biomedcentral.com)
  • First, we are interested in Epstein Barr Virus-mediated mechanisms of immune evasion with particular emphasis on pathways that promote survival and proliferation of EBV B cell lymphomas, the characterization of the human T cell and NK cell response to EBV and the identification of novel therapeutics for treatment of EBV B cell lymphomas. (stanford.edu)
Donors4
  • To compare the heterogeneities of human umbilical cord mesenchymal stem cells (HUCMSCs) derived from different donors and test their therapeutic variations in mouse liver fibrosis model. (researchsquare.com)
  • Most CAR-T cell-based gene therapy products that are under clinical evaluation consist of autologous enriched T cells, whereas CAR-NK cell-based approaches can be generated from allogeneic donors. (nature.com)
  • Among PSCs, the donors available for induced pluripotent stem cells (iPSCs) are greatest, providing a potentially universal cell source for all types of cell therapies including cancer immunotherapies using natural killer (NK cells). (molcells.org)
  • But another factor parents need to consider, is that stem cell transplants have fewer complications and better survival rates with donors who are not just a match on the HLA types, but who are related to the patient. (biohellenika.gr)
Pluripotent7
Lineages2
  • All stem cells are undifferentiated cells that exhibit unlimited self-renewal and can generate multiple cell lineages or more restricted progenitor populations that can contribute to tissue homeostasis by replenishing the cells or to tissue regeneration after injury. (biomedcentral.com)
  • Progenitor cell therapy describes the use of multipotent cells of various cell lineages (autologous or allogeneic) for tissue repair and/or regeneration. (southcarolinablues.com)
Vivo5
  • Autoimmune side effects, however, remain a risk, emphasizing the relevance of a suicide mechanism allowing in vivo elimination of infused T cells. (haematologica.org)
  • Adverse side effects, however, may occur and lead to severe autoimmunity or graft-versus-host disease (GvHD), emphasizing the relevance of a suicide mechanism that allows efficient in vivo elimination of infused T cells. (haematologica.org)
  • Transfer of HSV-tk to DLI preserves the beneficial anti-tumor effect and allows in vivo elimination of donor T cells if severe GvHD occurs. (haematologica.org)
  • A dose escalation study was performed to determine in vivo efficacy of IgA3.0 ch14.18 in an intraperitoneal mouse model using 9464D-GD2 neuroblastoma cells as well as in a subcutaneous human xenograft model using IMR32 neuroblastoma cells. (bmj.com)
  • Peptides were synthesized and tested for in vivo immunogenicity by screening peripheral blood mononuclear cells from healthy volunteers (n = 64) and HAdV-infected stem cell recipients (n = 26) for memory T cells recognizing the candidate epitopes in the context of most common HLA alleles. (biomedcentral.com)
HSCT4
Therapy in retinal1
Tissues9
  • hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
  • Donor T cells may also induce GvHD due to recognition of allo-antigens on non-malignant tissues of the patient. (haematologica.org)
  • Autoimmune diseases are usually complex and multifactorial, characterized by aberrant production of autoreactive immune cells and/or autoantibodies against healthy cells and tissues. (frontiersin.org)
  • Autoimmune diseases are complex diseases characterized by the loss of immunological tolerance to self-antigens and sustained aberrant immunological response against healthy cells and tissues, leading ultimately to the overproduction of autoreactive immune cells and/or autoantibodies ( 1 , 2 ). (frontiersin.org)
  • Organs and tissues are built up by specialized cells from the pool of stem cells that form shortly after fertilization. (bmrat.org)
  • Treating such diseases remains difficult and several strategies have been used to stimulate the growth of blood vessels and promote regeneration of ischaemic tissues, such as the use of recombinant proteins and gene therapy. (biomedcentral.com)
  • Ischaemia is characterised by a reduction in oxygen supply to tissues and organs, usually as a result of blood vessel constriction or obstruction. (biomedcentral.com)
  • The evaluation of the most suitable timing of cell delivery as well as the number of cells needed to integrate into resident vasculature and promote revascularisation of specific tissues requires careful optimisation and evaluation. (biomedcentral.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Umbilical1
  • When the savior child is born, its umbilical cord blood can be saved as a source of stem cells for the patient in need of a transplant. (biohellenika.gr)
Nonmyeloablative1
Donor4
  • 1 , 2 The anti-tumor effect of DLI is mediated by donor T cells recognizing allo-antigens on the malignant cells of the recipient. (haematologica.org)
  • Allogeneic-donor-derived cells can be genetically modified to eliminate expression of HLA-A. (ashpublications.org)
  • HLA-A disruption from donor cells is a step toward generating allogeneic cells as an off-the-shelf therapeutic. (ashpublications.org)
  • For those families with a child that requires a stem cell transplant, often there is the additional hurdle of finding a donor for the transplant. (biohellenika.gr)
Therapies4
  • Chimeric antigen receptor (CAR)-T cell therapies are on the verge of becoming powerful immunotherapeutic tools for combating hematological diseases confronted with pressing medical needs. (nature.com)
  • Lately, CAR-NK cell therapies have also come into focus as novel therapeutic options to address hurdles related to CAR-T cell therapies, such as therapy-induced side effects. (nature.com)
  • In this review, we are going to take a closer look at the commercial CAR-T cell therapies, as well as on CAR-T and CAR-NK cell products, which are currently under evaluation in clinical trials, that are being conducted in Germany. (nature.com)
  • There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
Renal cell carc4
  • The therapeutic approach to renal cell carcinoma (RCC) is guided by the probability of cure, which is related directly to the stage or degree of tumor dissemination. (medscape.com)
  • Go to Renal Cell Carcinoma Treatment Protocols , Clear Cell Renal Cell Carcinoma , and Sarcomatoid and Rhabdoid Renal Cell Carcinoma for complete information on these topics. (medscape.com)
  • To view a multidisciplinary tumor board case discussion, see Memorial Sloan Kettering e-Tumor Boards: Metastatic Clear Cell Renal Cell Carcinoma . (medscape.com)
  • Surgical resection remains the only known effective treatment for localized renal cell carcinoma, and it also is used for palliation in metastatic disease. (medscape.com)
Genetically modified1
  • The use of CD20 as suicide gene was investigated in CMV specific T cells and in T cells genetically modified with an antigen specific T-cell receptor. (haematologica.org)
20162
Progenitor cell therapy6
  • Progenitor cell therapy is being investigated for the treatment of damaged myocardium resulting from acute or chronic cardiac ischemia and for refractory angina. (southcarolinablues.com)
  • For individuals who have chronic cardiac ischemia who receive progenitor cell therapy, the evidence includes a nonrandomized comparative trial and systematic reviews of smaller RCTs. (southcarolinablues.com)
  • For individuals who have refractory angina who receive progenitor cell therapy, the evidence includes phase 2 trials and a phase 3 pivotal trial. (southcarolinablues.com)
  • In preclinical and clinical studies, progenitor cell therapy (cord blood and mesenchymal stem cells) has shown promise in reversing the underlying pathology of SNHL, the loss of cochlear sensory hair cells. (intechopen.com)
  • Progenitor cell therapy may also allow functional reorganization of the auditory pathways including primary auditory cortex (Heschl's gyrus). (intechopen.com)
  • We will present a summary of the effect of hearing loss on auditory development, existing preclinical and clinical data on progenitor cell therapy, and its potential role in the (re)habilitation of non-genetic SNHL. (intechopen.com)
Chemotherapy1
  • Options for chemotherapy and endocrine-based approaches are limited, and no hormonal or chemotherapeutic regimen is accepted as a standard of care. (medscape.com)
Signaling pathway1
  • The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis found 16 significant regulated pathways in imDCs and 10 significant regulated pathways in mDCs, including the phagosome, cell adhesion signaling pathway, and inflammatory mediator regulation of TRP channels pathway. (frontiersin.org)
Subpopulations2
  • In experimental and clinical studies unselected mononuclear bone marrow cells as well as specific subpopulations have been used for transplantation. (escardio.org)
  • After an incubation period of 6 days, the proportions of the T-cell subpopulations TEMRA (CD45RA + CD27 − ), memory (CD45RA − CD27 + ), effector (CD45RA − CD27 − ) and naive cells (CD45RA + CD27 + ) were determined. (biomedcentral.com)
Diseases12
  • Endothelial progenitor cells (EPCs) contribute to neovascularization and play an important role in the development of these diseases. (hindawi.com)
  • In each of these diseases, genes that play a role in the proliferation or activation of CD8+ T cells have been found to be affected by epigenetic modifications. (frontiersin.org)
  • However, mounting evidence has suggested that CD8+ T cells, in particular, play an important role in the induction, progression, pathogenesis, and protection for autoimmune diseases ( 5 ). (frontiersin.org)
  • In autoimmune diseases, these target cells killed by autoreactive CD8+ T cells can release numerous autoantigens to induce the overproduction of autoantibodies, and finally lead to the death of self-cells. (frontiersin.org)
  • Dendritic cells (DCs), which are the most important antigen-presenting cells (APCs) that send signals to the T cells, mainly participate in the pathogenesis of many diseases with immunoregulatory mechanisms, such as AR. (frontiersin.org)
  • This is a problem for the therapeutic use of recombinant TNFSF ligands in diverse diseases inclu. (biomedcentral.com)
  • The prognosis of the disease may vary differentially based on the causes and conditions of diseases. (ijpsonline.com)
  • Large numbers of myeloid-derived suppressor cells (MDSCs), a mixture of monocytic and granulocytic cells, accumulate during many pathologic conditions, including cancer, infectious diseases, trauma, and sepsis. (biomedcentral.com)
  • Ischaemia-related diseases such as peripheral artery disease and coronary heart disease constitute a major issue in medicine as they affect millions of individuals each year and represent a considerable economic burden to healthcare systems. (biomedcentral.com)
  • In particular, we focus on the therapeutic potential of endothelial progenitor cells as an exciting new option for the treatment of ischaemic diseases. (biomedcentral.com)
  • His pioneering work spans discoveries in basic biology of blood stem cells and leukemia to first-in-human clinical trials for people with cancer and heart diseases, alongside shaping innovative policies in state health and healthcare financing. (ufl.edu)
  • Solid organ transplantation is an important therapeutic option for children with a variety of end stage diseases. (stanford.edu)
Antigen-specific1
  • Transduction of CLL cells by CD40 ligand enhances an antigen-specific immune recognition by autologous T cells. (shengsci.com)
Regeneration3
  • The idea of myocardial regeneration with stem cell transplantation after myocardial infarction receives tremendous interest. (escardio.org)
  • The use of cell therapy for vascular regeneration offers an exciting new prospect in regenerative medicine. (biomedcentral.com)
  • 1 Treatment with progenitor cells (i.e., stem cells) offers potential benefits beyond those of standard medical care, including the potential for repair and/or regeneration of damaged myocardium. (southcarolinablues.com)
Human4
  • Enhancement of human erythroid progenitor cell growth by media conditioned by a human t-lymphocyte line. (shengsci.com)
  • Sadan O, Melamed E, Offen D. Intrastriatal transplantation of neurotrophic factor-secreting human mesenchymal stem cells improves motor function and extends survival in R6/2 transgenic mouse model for Huntington's disease. (jamanetwork.com)
  • Understanding heterogeneity of human bone marrow plasma cell maturation and survival pathways by single-cell analyses. (immunetolerance.org)
  • IgA3.0 ch14.18 was evaluated in binding assays and in ADCC and antibody-dependent cellular phagocytosis (ADCP) assays with human, neuroblastoma patient and non-human primate effector cells. (bmj.com)
Clinical studies2
  • The aims of this article are to review the current knowledge regarding therapeutic mechanisms of mesenchymal stem cells in acute liver failure, to discuss recent advancements in preclinical and clinical studies in the treatment of mesenchymal stem cells, and to summarize the methodological improvement of mesenchymal stem cell transplantation in treating liver failure. (springer.com)
  • A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
Anti-inflammatory1
  • Thus, the basic therapeutic goal in ADs is to achieve immune homeostasis, i.e., the state of dynamic equilibrium, a balance between pro- and anti-inflammatory responses. (mdpi.com)
Multiple myeloma1
Survival2
  • Reversible proteasome inhibition disrupts pathways supporting cell growth, thus decreases cancer cell survival. (medscape.com)
  • Intravenous mesenchymal stem cells improve survival and motor function in experimental amyotrophic lateral sclerosis. (jamanetwork.com)
Cellular4
  • Many of these factors stem from the location of the epitope that can affect accessibility of the antibody to the epitope at a cellular or molecular level, direct inhibition of target antigen activity, conservation of function despite escape mutations, and even non-competitive inhibition sites. (preprints.org)
  • This complex degrades ubiquitinated proteins that control the cell cycle and cellular processes and maintains cellular homeostasis. (medscape.com)
  • These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
  • Apheresis refers to the process of separating the cellular and soluble components of blood using a machine. (msdmanuals.com)
Cord blood6
  • While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
  • Placenta-cord blood can save lives. (biohellenika.gr)
  • Expecting parents mut be informed about the current and future use of cord blood stem cells and decide for family use or for public donation. (biohellenika.gr)
  • The new program is open to children that have a diagnosis of cerebral palsy and also have their own cord blood in storage. (biohellenika.gr)
  • The first expanded access program (EAP) of cord blood stem cell therapy for cerebral palsy launched in the United States at Duke University in Oct. 2017, under FDA registration as clinical trial NCT03327467. (biohellenika.gr)
  • At the Cord Blood Association annual meeting in Sept 2019, Dr. Joanne Kurtzberg of Duke stated in her talk that to date over 320 children have received treatment through this program. (biohellenika.gr)
Innate1
  • On the other hand, other ligands, such as HLA, bind to inhibitory receptors NKG2A and killer immunoglobulin-like receptors (KIR) to inhibit NK cells, providing a form of protection for self-cells from innate immunity. (molcells.org)
Immune cells4
  • Adaptive immune cells, including B and T cells, have been demonstrated to be primary contributors to the overactive immune response and overproduction of antibodies. (frontiersin.org)
  • One way to achieve this is to genetically modify immune cells, mainly T cells and recently also natural killer (NK) cells, to express chimeric antigen receptors (CARs). (nature.com)
  • The addition of separate adapter molecules (AMs) specific for tumor antigens and CAR-immune cells targeting these AMs allows a more precise and temporally limited therapy. (nature.com)
  • CAR-modified immune cells are expanded until sufficient cell numbers are attained and are adoptively transferred into the patient to fight malignant cells. (nature.com)