Cell apoptosis protein clone cells. Medical search. Frequent questions

Cell apoptosis was analyzed by flow cytometry. (jcancer.org)
S.W. Sherwood and R.T. Schimke, Cell Cycle Analysis of Apoptosis Using Flow Cytometry. (nhbs.com)
Potential roles of AKBA in regulating the cell cycle, apoptosis, and autophagy in A549 were evaluated by flow cytometry, Western blotting, reverse transcription-polymerase chain reaction (PCR) and immunofluorescence (IF). (boswellia.org)
The colony-forming ability of the cells was evaluated using plate cloning assay, and cell cycle changes and cell apoptosis were analyzed with flow cytometry. (bvsalud.org)

Here we show that induction of p53 causes an activation of NF-kappaB that correlates with the ability of p53 to induce apoptosis. (nih.gov)

The proliferation and growth of PDAC cells were detected by Celigo analysis, MTT, and clone formation assay. (jcancer.org)
The effects of AKBA on the cell viability in A549, H460, H1299, and BEAS-2B cells were determined by the CCK-8 assay. (boswellia.org)
The colony formation assay was used to identify the effects of AKBA on cell proliferation. (boswellia.org)
The number of live RGCs was then counted using a cell viability assay. (molvis.org)
Putative CSCs were isolated from human melanoma cells of a different aggressiveness, Hs294T and A375 cell lines, grown under hypoxia using "sphere-forming assay", CD133 surface expression and migration ability. (oncotarget.com)
C1QBP knockdown and overexpression cells were established via lentiviral infection and subjected to apoptosis and ROS assay in vitro . (medsci.org)
The localization pattern of capsid expression was analyzed by immunofluorescent assay in HeLa, 293-T, RD, or SH-SY5Y cells by using anti-His tag antibody as described ( 6 ). (cdc.gov)
In vitro apoptosis in individual cells was determined by terminal desoxyriboxyl-desoxyriboxyl transferase-mediated DVTP nick-end labeling (TUNEL) assay with the In Situ Cell Death Assay Kit (Roche Diagnostic Corp., Indianapolis, IN) and visualized by fluorescent microscopy. (cdc.gov)

Current data suggest that oral lichen planus (OLP) is a T-cell-mediated autoimmune disease in which autocytotoxic CD8 + T cells trigger the apoptosis of oral epithelial cells. (medscape.com)

It possesses a unique forkhead domain combined with a fragment of the target gene to initiate transcription [ 10 ] and regulates various biological processes such as tumor cell differentiation, cell proliferation, and migration [ 11 ]. (hindawi.com)
TIA1- and TIAL1-deficient GC B cells fail to undergo antigen-mediated positive selection, expansion and differentiation into B-cell clones producing high-affinity antibodies. (ox.ac.uk)
Receptor tyrosine kinases (RTKs) play fundamental tasks in varied cell functions including proliferation differentiation survival migration and rate of metabolism (16). (exposed-skin-care.net)
May be involved in regulation of transcriptional responses to NF-kappa-B, including cell adhesion, immune and proinflammatory responses, apoptosis, differentiation and growth. (lu.se)

A.J. McGahon, S.J. Martin, R.P. Bissonnette, A. Mahboubi, Y. Shi, R.J. Mogil, W.K. Nishioka, and D.R. Green, The End of the (Cell) Line: Methods for the Study of Apoptosis In Vitro. (nhbs.com)
In this work, the purified fractions were tested in-vitro for LTC, LTA and COX-2 activities using ELISA and qPCR was performed to determine gene regulation in human leukemia (HL-60) Cells. (boswellia.org)
Soluble Axl (sAxl) is present in cell-conditioned medium of CUDC-305 (DEBIO-0932 ) tumor cells growing in vivo and in vitro and in the sera of humans mice and rats (23 50 However the identities of the sAxl-generating protease(s) and the mechanism(s) that account for this process remain unknown. (exposed-skin-care.net)
[ 8 ] T cells in oral lichen planus contain mRNA for TNF and secrete TNF in vitro. (medscape.com)
The DNMT3A-mutated AML cells attenuated M1 macrophage polarization and resisted its killing effect in vitro and in vivo . (frontiersin.org)
Qingjiao Liao , Jiansheng Tian , Yang Wu , Xulin Chen , 2013: The Identification of Three Sizes of Core Proteins during the Establishment of Persistent Hepatitis C Virus Infection in vitro , Virologica Sinica, 28, 129-135. (virosin.org)
We observed that the WNV-Cp protein is a pathogenic protein, which drives apoptosis in vitro through the mitochodrial/caspase-9 pathway. (cdc.gov)

The TAM receptor and ligand expression was evaluated in LMS cell lines and 358 sarcoma samples by either gene expression or immunohistochemistry. (nature.com)
High expression of TYRO3 and AXL was detected in LMS cell lines. (nature.com)
Expression of the human gene has been shown to be repressed by BCL6, a transcriptional repressor required for lymph node germinal center development, suggesting that BCL6 regulates apoptosis by its effects on PDCD2. (wikipedia.org)
RNA interference was used to inhibit the expression of PYCR1 in PANC- 1 and AsPC-1 cells. (jcancer.org)
After infection, the expression of PYCR1 protein was detected by Western blot. (jcancer.org)
SCARA5 expression is significantly downregulated in various tumor tissues including liver cancer (LC), rectal cancer, and glioblastoma [ 15 , 16 ] and might affect cell proliferation and invasion abilities by the focal adhesion kinase (FAK) signaling pathway and epithelial-mesenchymal transition (EMT) [ 5 , 17 - 20 ]. (hindawi.com)
In abstract, a framework was developed to supply useful human full-length GLP-1R by recombinant expression in E. coli as a prerequisite for eventual construction willpower and a rigorous biophysical characterization together with protein variants. (ncbcs.org)
qRT-PCR was used to detect the expression of miRNA-149 and FOXC1 in gastric cancer tissues and cells. (hindawi.com)
The knockouts were successfully created and the effectiveness of the CRIPSR silencing was proven by real time PCR to measure mRNA expression, and ELISA to measure KLK6 protein secretion. (arizona.edu)
The heterozygous clones however, showed either normal expression of KLK6, or complete silencing of the gene, verified by the same methods mentioned. (arizona.edu)
We found that AKBA suppressed the formation of autolysosome, and decreased the expression levels of Beclin-1, LC3A/B-I, and LC3A/B-II proteins. (boswellia.org)
Furthermore, AKBA also inhibited the expression levels of PI3K/Akt signaling pathway proteins. (boswellia.org)
Fas-activated DCs increase the expression of MHC II, B7 co-stimulatory molecules, and DC-lysosome-associated membrane proteins (DC-LAMP), and secrete proinflammatory cytokines such as IL-1β and TNF-α. (biolegend.com)
The effects of OSI-774 on expression of EGFR messenger (m)RNA and protein, proliferation, anchorage-independent growth, and apoptosis were examined using semiquantitative reverse transcription-polymerase chain reaction, immunocytochemical analysis, Coulter counting, soft agar cloning, and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling/fluorescence-activated cell sorting, respectively. (thejns.org)
In contrast, no considerable correlation could be established between baseline expression levels of EGFR (both mRNA and protein) in GBM cell lines and their biological response to OSI-774. (thejns.org)
Mechanistically, TIA1 and TIAL1 control the transcriptional identity of dark- and light-zone GC B cells and enable timely expression of the prosurvival molecule MCL1. (ox.ac.uk)
We found that a cell sub-population enriched for P1 sphere-initiating ability and CD133 expression also express larger amount of VEGF-R2. (oncotarget.com)
OBJECTIVE@#To investigate the effect of lactic acid-induced upregulation of PLEKHA4 expression on biological behaviors of glioma cells and the possible molecular mechanism. (bvsalud.org)
The mRNA expression of PLEKHA4 was detected by PCR in glioma samples and controls and in glioma cells treated with lactic acid and glucose. (bvsalud.org)
Infection efficiency of the adenovirus vector and expression level of REIC/Dkk-3 in the resistant clones were similar to those in the parental PC3 cells. (elsevierpure.com)
Expression levels of BiP and rates of apoptosis induced by Ad-REIC were inversely correlated. (elsevierpure.com)
Genetic mutations in ATRX, DAXX, and H3.3 have been detected in ALT positive cancers, however, a subset of ALT samples show loss of ATRX or DAXX protein expression or localization without evidence of genetic alterations suggesting additional uncharacterized defects in ATRX/DAXX/H3.3 function. (oncotarget.com)
JPH203 interfered with constitutive activation of mTORC1 and Akt, decreased expression of c-myc and triggered an unfolded protein response mediated by the C/EBP homologous protein (CHOP) transcription factor associated with cell death. (c3m-nice.fr)
Virtually absent from normal pediatric and adult tissues, with the exception of low-level expression in a subset of immature b cell precursors known as hematogones and adipocytes, ROR1 is notably overexpressed, and considered a survival factor, in a number of B lymphoid and epithelial malignancies: including chronic lymphocytic leukemia (CLL), mantle cell lymphoma (MCL), acute lymphoblastic leukemia (ALL), marginal zone lymphoma, lung adenocarcinoma. (peprotech.com)
After the co-culture, the increase in pro-inflammatory cytokine expression in the mutant cells was significantly lower than that in the control group, while that in immunosuppressive factors was not significantly different. (frontiersin.org)
Resistin significantly promoted the expression of inflammatory proteins in AML cells. (frontiersin.org)
The degree of methylation affects the expression of target genes and has a significant impact on cell proliferation, apoptosis, cytokine secretion, and cell activity ( 6 , 7 ). (frontiersin.org)
C1QBP promoted the mRNA and protein expression of hypoxanthine catabolic enzyme XDH. (medsci.org)
C1QBP significantly increased ROS level, apoptosis, and the expression of apoptotic proteins such as cleaved caspase-3 and bax/bcl2 via regulating XDH. (medsci.org)
王平忠 , 白雪帆 , 潘蕾 , 张颖 , 黄长形 , 2003: Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome, Virologica Sinica, 18, 420-422. (virosin.org)
刘超红 , 焦成松 , 陈新文 , 2006: Cloning of HCV ns2 Gene and its Expression in Prokaryotic and Mammalian cells, Virologica Sinica, 21, 217-220. (virosin.org)
We also observed that expression of Cp protein in mouse muscle resulted in apoptosis and inflammation of muscle cells. (cdc.gov)
More importantly, direct in vivo expression of WNV-Cp protein in mouse brain resulted in an induction of apoptosis similar to what is observed in natural infection. (cdc.gov)
Younes F, Quartey EL, Kiguwa S, Partridge M. Expression of TNF and the 55-kDa TNF receptor in epidermis, oral mucosa, lichen planus and squamous cell carcinoma. (medscape.com)
Our results suggest that although trisomy 8 cells are in a pro-apoptotic state, they are checked by the enhanced expression of anti-apoptotic signals which provide them with their proliferative advantage. (who.int)

Inhibition of cytotoxic effect of FASL on Jurkat cells in the presence of recombinant human FAS. (biolegend.com)
ED 50 = 10 - 50 ng/ml, corresponding to a specific activity of 0.02 - 0.1 x 10 6 units/mg, as determined by inhibition of apoptotic cell death induced by FASL on Jurkat cells. (biolegend.com)
Inhibition or loss of NF-kappaB activity abrogated p53-induced apoptosis, indicating that NF-kappaB is essential in p53-mediated cell death. (nih.gov)
Inhibition of MEK1 blocked activation of NF-kappaB by p53 and completely abrogated p53-induced cell death. (nih.gov)
Just clones with shRNA concentrating on cIAP-1 had been sensitized to TRAIL-mediated apoptosis, whereas cIAP-2 or XIAP mobile depletion acquired no significant influence on apoptosis SVT-40776 inhibition (Fig. 2BCC). (acancerjourney.info)

KLK6 is a secreted protein commonly overexpressed in CRC due to its effects on cell growth, apoptosis and metastasis. (arizona.edu)
Analysis of the changes of migration and invasion, showed significant reduced LAPTM5 suppressed cell metastasis. (spandidos-publications.com)
Furthermore, proteins involved in epithelial-mesenchymal transition (EMT) were strongly altered, which plays a central role in metastasis. (spandidos-publications.com)
patients with type E (AD) tumor had higher incidence of nodal metastasis at MGCD0103 cell line TGF-beta inhibitor mediastinal lymph node than did patients in tumor groups, and all mediastinal positive nodes existed in lower mediastinal area. (hsdpathway.com)

In human BCa tissues, we observed that LAPTM5 was significantly induced at both mRNA and protein levels, which is consistent with our microarray result. (spandidos-publications.com)
mRNA and protein expressions of Ngb and HIF-1α showed positive in different tissues of the yak and cattle telencephalon. (bvsalud.org)

Subsequently, caspase 8 is released from DISC and induces activation of caspase 3 and 7, proteolysis of cellular components, and apoptosis. (biolegend.com)
The interaction of FAS/FASL in immature dendritic cells (DCs) induces functional maturation. (biolegend.com)
Activation or re-introduction of p53 induces apoptosis in many tumour cells and may provide effective cancer therapy. (nih.gov)
We report that the WNV-capsid (Cp) by itself induces rapid nuclear condensation and cell death in tissue culture. (cdc.gov)

Recent studies have shown that in addition to necrosis and apoptosis, there are also other new programmed death modes, such as autophagy, necrosis and necrotic apoptosis, which have unique biological processes and pathophysiological characteristics. (nature.com)
Acetyl-11-Keto-β-Boswellic Acid Exerts the Anti-Cancer Effects via Cell Cycle Arrest, Apoptosis Induction and Autophagy Suppression in Non-Small Cell Lung Cancer Cells. (boswellia.org)
AKBA exerts the anti-cancer effects via cell cycle arrest, apoptosis induction, and autophagy suppression in NSCLC cells. (boswellia.org)
We show here that a potent and LAT1 selective inhibitor (JPH203) decreased leukemic cell viability and proliferation, and induced transient autophagy followed by apoptosis. (c3m-nice.fr)

TYRO3 or AXL gene knockdown reduced cell proliferation/colony formation. (nature.com)
Crizotinib and foretinib decreased TYRO3 and AXL phosphorylation, apoptosis, G2/arrest and reduced colony formation. (nature.com)
In addition, FAS/FASL interaction increases the production of chemokines in NK-T cells, and FASL acts as a survival factor for human CD34 + cells and increases their colony formation. (biolegend.com)
Trisomy 8+ cells showed a significant positive correlation with apoptotic CD34+ cells and capacity for colony formation. (who.int)

Ferroptosis-inducing factors can directly or indirectly affect glutathione peroxidase through different pathways, resulting in a decrease in antioxidant capacity and accumulation of lipid reactive oxygen species (ROS) in cells, ultimately leading to oxidative cell death. (nature.com)
The purpose of this study was to investigate whether astaxanthin confers a neuroprotective effect against glutamate stress, oxidative stress, and hypoxia-induced apoptotic or necrotic cell death in primary cultures of rat retinal ganglion cells (RGCs). (molvis.org)
Our results suggest that astaxanthin has a neuroprotective effect against RGC death induced by glutamate stress, oxidative stress, and hypoxia, which induce apoptotic and necrotic cell death. (molvis.org)
In this study, we investigate the influence of AST against glutamate- and hypoxia-induced apoptosis, as well as oxidative stress-induced necrosis, in primary rat RGC cultures. (molvis.org)
However, the role of C1QBP in metabolism, oxidative stress, and apoptosis of renal cell carcinoma (RCC) cells have not yet been explored. (medsci.org)

Strong induction of apoptosis in one compartment of the Drosophila wing disc causes apoptosis of cells in the other compartment, indicating that dying cells can release long-range death factors. (elifesciences.org)
The induction of EGFR mRNA may constitute a cellular mechanism to counteract the inhibitory effect of OSI-774 on the anchorage-independent growth of GBM cells. (thejns.org)
Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
A JPH203-resistant tPTEN-/-clone appeared CHOP induction deficient. (c3m-nice.fr)
These studies demonstrate that the capsid protein of WNV may be responsible for aspects of viral pathogenesis through induction of the apoptotic cascade. (cdc.gov)

X-linked inhibitor of apoptosis (XIAP) is the most potent endogenous caspase inhibitor preventing cell death via caspase-9, -7 and -3 (initiator and executioner caspase pathways). (nih.gov)
XIAP knockdown induced a 2-fold increase in the basal level of apoptosis without modification of caspase 3/7 activity. (nih.gov)
Cell-free studies confirmed cIAP-1 can be a substrate for caspase 8, with most likely multiple cleavage sites. (acancerjourney.info)
These outcomes claim that TRAIL-mediated apoptosis proceeds SVT-40776 through caspase 8-reliant degradation of cIAP-1. (acancerjourney.info)
E,F) Mz-ChA-1 had been treated with raising concentrations of Path (0C20 ng/ml) and apoptosis was evaluated by (E) fluorescence microscopy and (F) SVT-40776 caspase 3/7 activation. (acancerjourney.info)
Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. (thermofisher.com)
Apoptosis is induced through the mitochondrial pathway resulting in caspase-9 activation and downstream caspase-3 activation. (cdc.gov)
For a caspase-9-specific test, 5 μg of pcWNV-Cp-DJY or pcWNV-CpWT was cotransfected with a dominant negative caspase-9 (DN caspase-9) construct, and cleavage of procaspase-9 protein was determined by Western blot analysis with antihuman caspase-9 antibody (MBL, Nagoya, Japan). (cdc.gov)

B.A. Osborne, S.W. Smith, Z.-G. Liu, K.A. McLaughlin, and L.M. Schwartz, Transient Transfection Assays to Examine the Requirement of Putative Cell Death Genes. (nhbs.com)
Therefore, it is important to explore candidate genes that could have significance to the clinical diagnosis, as well as the treatment of esophageal squamous cell carcinoma (ESCC) at the molecular level [ 4 ]. (hindawi.com)
The proteins produced from both genes are involved in a multistep process that connects particular proteins to the surface of cells. (medlineplus.gov)

XIAP mRNA levels were established by RT-PCR, the four X (XIAP knockdown) clonal cell lines show 82-93% reduction in XIAP mRNA when compared to the four L (luciferase control) cell lines. (nih.gov)
Immunoblot analysis showed a 67-89% reduction in XIAP protein in X cell lines compared to L. RNA was analysed by microarray and XIAP knockdown was confirmed in 7 probe sets, there was no significant compensation of other IAP family members. (nih.gov)
Two clones with effective knockdown of every proteins had been selected and used for these research (Fig. 2A). (acancerjourney.info)

Protein tyrosine (pTyr) phosphorylation is a common post-translational modification which can create novel recognition motifs for protein interactions and cellular localisation, affect protein stability, and regulate enzyme activity. (embl.de)
Consequently, maintaining an appropriate level of protein tyrosine phosphorylation is essential for many cellular functions. (embl.de)
Upon immobilization sAxl advertised cell migration and induced the phosphorylation of Axl and phosphatidylinositol 3-kinase. (exposed-skin-care.net)
Western blotting was used to detect the expressions of cyclinD1, CDK2, Bcl2, β-catenin and phosphorylation of the key proteins in the MAPK signaling pathway. (bvsalud.org)

Eiger is produced in apoptotic cells and, through activation of the c-Jun N-terminal kinase (JNK) pathway, is able to propagate the initial apoptotic stimulus. (elifesciences.org)
found that the JNK pathway activated apoptosis in anterior cells. (elifesciences.org)
While the precise mechanism underlying activation of the ALT pathway is unclear, mutations in the chromatin remodeling protein ATRX, histone chaperone DAXX, and the histone variant H3.3 correlate with ALT status. (oncotarget.com)
The majority of cancer cells rely on reactivation of the enzyme telomerase or activation of the Alternative Lengthening of Telomeres pathway (ALT) to promote telomere elongation. (oncotarget.com)
In the poster section, Joanna Shisler (University of Illinois at Urbana-Champaign [UIUC], Urbana) reported that the modified virus, Ankara, activates nuclear factor κB through the mitogen-activated protein kinase, extracellular signal–regulated kinase (MEK)/extracellular signal–regulated kinase (ERK) pathway, possibly facilitating the host immune response. (cdc.gov)

The tumour suppressor p53 inhibits cell growth through activation of cell-cycle arrest and apoptosis, and most cancers have either mutation within the p53 gene or defects in the ability to induce p53. (nih.gov)
Moreover, cell cycle arrest at G0/G1 phase was triggered by decreased LAPTM5 as well, which could lead to delayed BCa cell growth. (spandidos-publications.com)
Taken together, our results suggested that decreased LAPTM5 inhibited proliferation and viability, as well as induced G0/G1 cell cycle arrest possibly via deactivation of ERK1/2 and p38 in BCa cells. (spandidos-publications.com)

Gas6 is the product of growth arrest-specific gene 6 which was in the beginning cloned from serum-starved fibroblasts and shares about CUDC-305 (DEBIO-0932 ) 44% sequence identity and related website organization with protein S a negative regulator of blood coagulation (48). (exposed-skin-care.net)

The OSI-774 induced greater ( p53 -independent) apoptosis in more malignant GBM phenotypes and may be a promising therapeutic agent against secondary GBM. (thejns.org)
Moreover, some studies demonstrated that LAPTM5 was highly expressed in malignant B lymphomas and involved in B cell malignancies ( 10 ), involving in negative regulation of cell surface T and B cell receptor by promoting lysosome degradation ( 6 ). (spandidos-publications.com)
Indeed, a 5-year survival rate of advanced stage malignant melanoma is less than 10% and resistance to chemotherapy, most likely due to the presence of cancer stem cells (CSCs), is the major cause of treatment failure [ 1 - 3 ]. (oncotarget.com)
Clear cell RCC (ccRCC) is the most common subtype among the malignant RCCs, accounting for 70-80%, followed by papillary (15%), chromophobe (4-5%), and other fewer subtypes[ 2 ]. (medsci.org)
Chronic myeloid leukemia (CML) occurs when a pluripotent stem cell undergoes malignant transformation and clonal myeloproliferation, leading to a striking overproduction of mature and immature granulocytes. (msdmanuals.com)

SCARA5 promoted cell proliferation and migration but inhibited apoptosis in ESCC. (hindawi.com)
miRNA-149 was underexpressed in gastric cancer tissues and cells, while overexpression of miRNA-149 promoted cell apoptosis, retarded cell cycle, and inhibited proliferation and migration in AGS and MKN28 cells. (hindawi.com)

Fractions of Suppress LTA, LTC, Cyclooxygenase-2 Activities and mRNA in HL-60 Cells and Reduce Lung Inflammation in BALB/c Mice. (boswellia.org)
Capsid gene delivery into the striatum of mouse brain or interskeletal muscle resulted in cell death and inflammation, likely through capsid-induced apoptosis in vivo. (cdc.gov)
These pro- has been implicated as contributing apoptosis, inflammation, and several liferating cel s replace dead cel s to the cancer phenotype, through pleiotropic responses. (who.int)

Programmed cell death protein 2 is a protein that in humans is encoded by the PDCD2 gene. (wikipedia.org)
This gene encodes a nuclear protein expressed in a variety of tissues. (wikipedia.org)
This gene is closely linked on chromosome 6 to the gene for TBP, the TATA binding protein. (wikipedia.org)
And defects in the PYCR1 gene can lead to the abnormality of mitochondrial membrane potential, which results in mitochondrial network disruption and the apoptosis rate increasing during oxidation [ 12 - 15 ]. (jcancer.org)
Because of breakthroughs such as the "switching" mechanism of the p53 tumor suppressor gene, as well as many technical innovations, the number of investigators of cell death has rapidly expanded over the last few years. (nhbs.com)
GSE76211) ( 3 , 4 ), revealing a significantly upregulated gene in BCa tissues, the lysosomal-associated protein multispanning transmembrane 5 (LAPTM5). (spandidos-publications.com)
Here, using Next Generation Sequencing we identified a novel gene fusion event between DAXX and the kinesin motor protein, KIFC3, leading to the translation of a chimeric DAXX-KIFC3 fusion protein. (oncotarget.com)
The gene (SLC7A5) encoding system L amino-acid transporter 1 (LAT1) is overexpressed in murine lymphoma cells generated via T-cell deletion of the pten tumor suppressor, and also in human T-cell acute lymphoblastic leukemia (T-ALL)/lymphoma (T-LL) cells. (c3m-nice.fr)
The cloning of a synthetic WNV-Cp gene based on the reported NY-99 infectious strain was described earlier ( 4 ). (cdc.gov)
In people with PNH, variants of the PIGA gene occur during a person's lifetime and are present only in certain cells. (medlineplus.gov)
PNH occurs when a somatic variant of the PIGA gene or PIGT gene occurs in a blood-forming cell called a hematopoietic stem cell. (medlineplus.gov)

Thus, we demonstrate here that TIA1 and TIAL1 are key players in the post-transcriptional program that selects high-affinity antigen-specific GC B cells. (ox.ac.uk)
Therefore, early in the formation of oral lichen planus lesions, CD8 + T cells may recognize an antigen associated with the major histocompatibility complex (MHC) class I on keratinocytes. (medscape.com)
After antigen recognition and activation, CD8 + cytotoxic T cells may trigger keratinocyte apoptosis. (medscape.com)
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)

Traditionally, cell death has been divided into apoptosis and necrosis. (nature.com)
It does not have the morphological characteristics of typical necrosis, such as swelling of the cytoplasm and organelles and rupture of the cell membrane, nor does it have the characteristics of traditional cell apoptosis, such as cell shrinkage, chromatin condensation, formation of apoptotic bodies and disintegration of the cytoskeleton. (nature.com)
We identified Eiger, the Drosophila tumor necrosis factor (TNF) homolog, as the signal responsible for apoptosis-induced apoptosis (AiA). (elifesciences.org)

IM combined with IR can synergistically inhibit the proliferation, apoptosis, clonogenesis, invasion, migration and cell cycle of GIST cells. (researchsquare.com)

The rat homolog, Rp8, is transiently expressed in immature thymocytes and is thought to be involved in programmed cell death. (wikipedia.org)
Ferroptosis is a new type of programmed cell death, which occurs with iron dependence. (nature.com)
Apoptosis is a distinct form of programmed cell death in which cells activate an intrinsic suicide program to self-destruct. (elifesciences.org)
The bcr-abl oncoprotein has uncontrolled tyrosine kinase activity, which deregulates cellular proliferation, decreases adherence of leukemia cells to the bone marrow stroma, and protects leukemic cells from normal programmed cell death (apoptosis). (msdmanuals.com)

Mouse anti PTEN tumour suppressor antibody, clone A2b1, is specific for PTEN, a tumour suppressor protein located on the human chromosome 10 that is often mutated in various types of advanced cancers. (bio-rad-antibodies.com)
We have recently shown that an adenovirus carrying REIC/Dkk-3 (Ad-REIC) exhibits a potent tumor-specific cell-killing function for various human cancers. (elsevierpure.com)
It has also become evident that some human cancers are resistant to Ad-REIC-induced apoptosis. (elsevierpure.com)
These data highlight a potentially unrecognized mechanism of DAXX inactivation in ALT positive osteosarcoma and provide rationale for thorough and comprehensive analyses of ATRX/DAXX/H3.3 proteins in ALT positive cancers. (oncotarget.com)
Renal cell carcinoma is one of the most common cancers in the urinary system, causing over 140,000 deaths worldwide each year[ 1 ]. (medsci.org)

deletion of an exon encoding the transmembrane domain results in a soluble FAS molecule that blocks apoptosis. (biolegend.com)
Several studies shown that Axl could mediate cell adhesion and aggregation through homotypic ectodomain associations (9 23 Both CUDC-305 (DEBIO-0932 ) murine and human being Axl proteins undergo proteolytic processing to yield a soluble form of this molecule. (exposed-skin-care.net)
We also demonstrate that targeting LAT1 may be an efficient broad spectrum adjuvant approach to treat deadly T-cell malignancies as the molecule synergized with rapamycin, dexamethasone, doxorubicin, velcade and l-asparaginase to alter leukemic cell viability. (c3m-nice.fr)
These proteins are attached to the cell by a specialized molecule called GPI anchor and are known as GPI-anchored proteins. (medlineplus.gov)

created 'undead' cells in which one of these proteins was turned on, but other downstream proteins (that are responsible for the cellular execution phase of apoptosis) had been turned off: these cells were undergoing apoptosis, but could not complete the process and die. (elifesciences.org)
In A549 cells, AKBA suppressed the clone formation, arrested the cell cycle at the G/G phase, induced cellular apoptosis. (boswellia.org)
The aim of the current study was to evaluate molecular and cellular effects of OSI-774, a novel EGFR tyrosine kinase inhibitor, on nine glioblastoma multiforme (GBM) cell lines. (thejns.org)
Outcomes Cellular depletion of cIAP-1 enhances the performance of TRAIL-mediated apoptosis We originally examined mobile degrees of cIAP-1, cIAP-2 and XIAP in the hepatocarcinoma cell series HuH-7 during treatment with raising concentrations of Path (0C20 ng/ml). (acancerjourney.info)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Telomere elongation is a requisite for cellular immortality and a hallmark of cancer cells. (oncotarget.com)
ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)

A highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinase (Ki = 140 nM for p160 ROCK (ROCK-I). Also inhibits ROCK-II with almost equal potency. (sigmaaldrich.com)
Does not affect the activity of p21-activated protein kinase (PAK) even at high concentrations (~100 µM). (sigmaaldrich.com)
Mammalian Cdc25 phosphatase is responsible for the dephosphorylation of Cdc2 and other cyclin-dependent kinases at Thr14 and Tyr15, thus activating the kinase and allowing cell cycle progression. (embl.de)
In addition, phosphorylated ERK1/2 and p38, key members of mitogen-activated protein kinase (MAPK) family regulating BCa tumorigenesis, were strongly decreased. (spandidos-publications.com)
Receptor-tyrosine-kinase-like orphan receptor 1 (ROR1) is a tumor-associated, surface protein predominantly expressed during embryogenesis, where it is involved in organ morphogenesis, nervous system development, and neural progenitor cell maintenance and survival. (peprotech.com)
First identified during PCR-based cloning of a human neuroblastoma cell line in search of tyrosine kinases similar to tropomyosin-receptor-kinase (Trk) neurotropic receptors, ROR1, along with the related receptor tyrosine kinase (RTK) ROR2, was catalogued as an "orphan" receptor due to the fact its related ligand remained elusive. (peprotech.com)

During EMT, cells will undergo transformation from epithelial phenotype to mesenchymal phenotype ( 14 ) and many characteristics of cells will change including loss of cell-cell adhesion and acquisition of aggressive and metastatic ability ( 15 ). (spandidos-publications.com)
Hypoxic melanoma cells sorted for VEGF-R2/CD133 positivity also undergo apoptosis when exposed to Etoposide and Bevacizumab. (oncotarget.com)

Enhances survival & cloning efficiency of ESC without affecting their pluripotency. (sigmaaldrich.com)
Prevents apoptosis and enhances the survival and cloning efficiency of dissociated hES cells without affecting their pluripotency. (sigmaaldrich.com)
In these cells, IL-21 does not promote proliferation but enhances apoptosis and chemotaxis. (intechopen.com)

PYCR1 interference was able to inhibit cell proliferation and promote cell apoptosis of pancreatic cancer. (jcancer.org)
Activation of the glucagon-like peptide-1 receptor (GLP-1R) upon ligand binding results in the discharge of insulin from pancreatic cells. (ncbcs.org)

this way T cells can check if the cell contains internal foreign proteins (sign of a virus infection or cancerous transformation). (stackexchange.com)

PTEN exhibits protein phosphatase activity and can suppress the growth of glioma cells. (bio-rad-antibodies.com)

This entry represents the PTPase domain found in several tyrosine-specific protein phosphatases (PTPases). (embl.de)

This process plays a major role in development and tissue homeostasis, and abnormal regulation of apoptosis is associated with a variety of human diseases ( Fuchs and Steller, 2011 ). (elifesciences.org)
Interleukin-21 (IL-21) is produced by activated T cells and it plays many diverse roles by regulating the functions of normal and abnormal cells. (intechopen.com)
CML ensues when an abnormal pluripotent hematopoietic progenitor cell initiates excessive production of all myeloid lineage cells, primarily in the bone marrow but also in extramedullary sites (eg, spleen, liver). (msdmanuals.com)

FAS is a type I transmembrane protein and belongs to the TNF receptor superfamily. (biolegend.com)

How to intervene in the occurrence and development of related diseases by regulating cell ferroptosis has become a hotspot and focus of etiological research and treatment, but the functional changes and specific molecular mechanisms of ferroptosis still need to be further explored. (nature.com)
This study aims to explore the roles and molecular mechanisms of AKBA on cell behavior in non-small cell lung cancer (NSCLC) cells. (boswellia.org)
The 397 amino acid recombinant protein has a predicted molecular mass of approximately 44.7 kD. (biolegend.com)

Right now, hematopoietic stem cell transplantation (HSCT) is the foremost dependable healing treatment with amazing comes about for patients with HLAmatched family or disconnected givers. (alliedacademies.org)
The WASP quality has 12 exons and encodes a 502 amino-acid protein (WASP), which is overwhelmingly communicated in nonerythroid hematopoietic cells [ 3 ]. (alliedacademies.org)
LAPMT5 is a lysosomal membrane protein preferentially expressed in immune cells ( 5 , 6 ) and hematopoietic cells ( 7 ), having a close interaction with the Nedd4 ( 8 ), a member of the E3 ubiquitin ligases family ( 8 ). (spandidos-publications.com)

FAS was initially purified and cloned from SKW6.4 cells and a cDNA library of human T cell lymphoma KT-3 cells. (biolegend.com)
L-type amino-acid transporter 1 (LAT1): a therapeutic target supporting growth and survival of T-cell lymphoblastic lymphoma/T-cell acute lymphoblastic leukemia. (c3m-nice.fr)

Ligand binding of the renatured protein was quantified by fluorescence quenching and floor plasmon resonance spectroscopy. (ncbcs.org)
A common heterophilic ligand for these RTK family members is definitely Gas6 a vitamin K-dependent protein that is widely secreted by most cells including the lungs intestine Serping1 and vascular endothelium (43). (exposed-skin-care.net)
Wnt-5a has since been suggested as a candidate ligand for ROR1, and ROR1 has been implicated to function as a pseudokinase, promoting proliferation and resistance to apoptosis in cancer cells through interaction with Wnt-5a, and TCL1-co-activation of AKT. (peprotech.com)

They can inhibit the growth of tumor cells, kill them via phagocytosis, and promote anti-tumor immunity. (frontiersin.org)

Ectodomain dropping has emerged as an important posttranslational mechanism to regulate the functions of various integral membrane-bound proteins including adhesion molecules cytokines growth factors and their receptors (57 60 Both. (exposed-skin-care.net)

The dense subepithelial mononuclear infiltrate in oral lichen planus is composed of T cells and macrophages, and there are increased numbers of intraepithelial T cells. (medscape.com)
The role of autoimmunity in the pathogenesis is supported by many autoimmune features of oral lichen planus, including its chronicity, onset in adults, predilection for females, association with other autoimmune diseases, occasional tissue-type associations, depressed immune-suppressor activity in patients with oral lichen planus, and the presence of autocytotoxic T-cell clones in lichen planus lesions. (medscape.com)
Sugerman PB, Satterwhite K, Bigby M. Autocytotoxic T-cell clones in lichen planus. (medscape.com)
Shan J, Ma JM, Wang R, Liu QL, Fan Y. Proliferation and Apoptosis of Peripheral Blood Mononuclear Cells in Patients with Oral Lichen Planus. (medscape.com)

Thus, removing MHC from the cells would make them very liable to become nearly unstoppable virus factories or tumor cells. (stackexchange.com)

Jurkat T cells were left untreated (upper left & lower left panels), treated for 5 hours (upper middle & lower middle panels) or 12 hours (upper right & lower right panels) with anti-human Fas antibody (clone DX2, Cat. (bdbiosciences.com)
By Western blot, this antibody recognizes a 116 kDa protein which corresponds to PARP and the 85 kDa apoptosis-induced cleavage product of prICE and CPP32 from rat thymus extract. (thermofisher.com)

Y-27632A, CAS 331752-47-7, is a cell-permeable, reversible, inhibitor of Rho kinases (K i = 140 nM for p160ROCK). (sigmaaldrich.com)
A highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinases (K i = 140 nM for p160 ROCK ). (sigmaaldrich.com)

In 2012, Dixon 1 first proposed the concept of ferroptosis, an iron-dependent, non-apoptotic mode of cell death characterized by the accumulation of lipid reactive oxygen species (ROS). (nature.com)

Immunologic variations from the norm of WAS incorporate T-cell lymphopenia, flawed proliferative reaction to CD3 cross-linking, disabled counter acting agent reaction to polysaccharide antigens, flawed monocyte chemotaxis, anomalies of fortified dendritic cells, and an expanded lymphocyte apoptosis with age. (alliedacademies.org)
In the blood-typing system, the AB antigens don't appear necessary for proper functioning of red blood cells - are the antigens on a person's own cells, actually needed for any essential functions, or could they be removed, without affecting that person's health? (stackexchange.com)
In this case, MHC1 antigens are required on cells for proper self-identification. (stackexchange.com)

We also show that during coordinated cell death of hair follicle cells in mice, TNF-α is expressed in apoptotic cells and is required for normal cell death. (elifesciences.org)
find that these cells can stimulate other cells to die in both fruit flies and mice. (elifesciences.org)
Eiger is related to a protein called TNF that has been implicated in cycles of destruction and renewal of hair follicles in mice. (elifesciences.org)
When Etoposide and Bevacizumab-treated hypoxic cells were injected intravenously into immunodeficient mice revealed a reduced capacity to induce lung colonies, which also appear with a longer latency period. (oncotarget.com)
JPH203 could also alter the in vivo growth of luciferase-expressing-tPTEN-/- cells xenografted into nude mice. (c3m-nice.fr)

Cell Death provides a current and comprehensive collection of methods for the study of cell death. (nhbs.com)
J.F.R. Kerr, G.C. Gobe, C.M. Winterford, and B.V. Harmon, Anatomical Methods in Cell Death. (nhbs.com)
汪艳 , 刘春新 , 易有荣 , 崔冶建 , 朱珺 , 董长垣 , 2006: Detection of Apoptosis of the Human Hepatic Carcinoma Cells Induced by Bluetongus Virus by PI and Annexin V/PI Staining Methods, Virologica Sinica, 21, 253-256. (virosin.org)

Here we created KLK6 knockouts and heterozygous clones from the SW480 cell line and measured cell growth and viability over multiple days. (arizona.edu)
The clones which did not express KLK6 showed much slower growth and viability compared to the cell lines which normally expressed the protein. (arizona.edu)

Down-regulation of BiP with siRNA sensitized the resistant cells to Ad-REIC in vivo as well as in culture. (elsevierpure.com)
These results provide evidence of a link between WNV-Cp protein and WNV pathogenesis in vivo. (cdc.gov)

Mutated recombinant human glucagon-like peptide-1 protects SH-SY5Y cells from apoptosis induced by amyloid-beta peptide (1-42). (ncbcs.org)
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. (biolegend.com)

L.T. Bemis, F.J. Geske, and R. Strange, Use of the Yeast Two-Hybrid System for Identifying the Cascade of Protein Interactions Resulting in Apoptotic Cell Death. (nhbs.com)
Other biological effects not leading to apoptotic cell death have also been described. (biolegend.com)

the silencing of FOXC1 inhibited the biological function of AGS and MKN28 cells. (hindawi.com)
In this study, we investigated the inhibitory effects of miR-149 on the biological functions of gastric cancer cells by targeting FOXC1. (hindawi.com)
However, we know less about the biological effects of IL-21 and its signaling mechanisms in leukemia cells and monocytes. (intechopen.com)
In this chapter, we will focus on IL-21's biological effects and signaling pathways as well as discuss the potential implications and applications of IL-21 in leukemia cells. (intechopen.com)

This current work goals to look at whether or not mGLP-1 attenuates Abeta(1-42)-mediated cytotoxicity in SH-SY5Y cells and to discover the attainable mechanisms. (ncbcs.org)
disabling their apoptosis mechanism (Cells with disabled apoptosis mechanisms are commonly referred to as cancer, so we might want to not do this. (stackexchange.com)
GC B cells rely on post-transcriptional RNA mechanisms to translate activation-associated transcriptional programs into functional changes in the cell proteome. (ox.ac.uk)
However, the critical proteins driving these key mechanisms are still unknown. (ox.ac.uk)
The signaling mechanisms of IL-21 in different cell types have been identified. (intechopen.com)

E. Wells containing endothelial cells with microspheres were analyzed for fluorescence. (hsdpathway.com)

All patients can evolve into trisomy 8+ MDS [12], cells, since acquiring adequate samples diagnosed with MDS have a reduced in which 8+ appears to confer a favour- for flow cytometric analysis renders the life expectancy compared with age- able prognosis [13]. (who.int)
The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)

He also showed that double-stranded (ds)RNA- and ZDNA binding proteins had a role in poxvirus pathogenesis. (cdc.gov)

Anatomy30

Organisms10

Diseases12

Chemicals and Drugs120

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes58

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science3

Flow cytometry4

Induce apoptosis1

Assay8

Epithelial cells1

Differentiation4

Vitro7

Expression35

Inhibition5

Metastasis4

MRNA and protein2

Induces4

Autophagy4

Colony formation4

Oxidative5

Induction5

Caspase8

Genes3

Knockdown3

Phosphorylation4

Pathway5

Cycle Arrest3

Arrest1

Malignant5

Proliferation and migration2

Inflammation3

Gene11

Antigen4

Necrosis3

Migration1

Programmed cell4

Cancers5

Molecule4

Cellular7

Kinase6

Undergo2

Enhances3

Pancreatic2

Infection1

Glioma cells1

Tyrosine1

Abnormal3

Transmembrane1

Molecular3

Hematopoietic3

Lymphoma2

Ligand3

Inhibit1

Adhesion1

Lichen planus4

Tumor cells1

Antibody2

Inhibitor2

Reactive oxygen1

Antigens3

Mice5

Methods3

Viability2

Vivo2

Recombinant2

Apoptotic Cell2

Biological4

Mechanisms5

Fluorescence1

Patients2

Pathogenesis1