• The specificity of APC ligases is proposed to be controlled by the incorporation of specificity factors into the ligase complex, instead of substrate phosphorylation. (wikipedia.org)
  • Activators CDC20 and Cdh1 are of particular significance and are the most widely studied and familiar of the APC/C subunits. (wikipedia.org)
  • These include Apc1, the largest subunit which contains 11 tandem repeats of 35-40 amino acid sequences, and Apc2, which contains three cullin repeats of approximately 130 amino acids total. (wikipedia.org)
  • It was the discovery of the APC/C (and SCF) and their key role in eukaryotic cell-cycle regulation that established the importance of ubiquitin-mediated proteolysis in cell biology. (wikipedia.org)
  • The subunit, CDC20 allows APC to degrade substrates such as anaphase inhibitors (Pdsp1) at the beginning of anaphase, on the other hand when CDC20 is substituted for specificity factor Hct1, APC degrades a different set of substrates, particularly mitosis cyclins in late anaphase. (wikipedia.org)
  • These domains, also found in Apc3, Apc7, and Apc8, have been shown to mediate protein-protein interactions, suggesting that Apc6 may assist in coordinating the juxtaposition of the catalytic and substrate recognition module subunits relative to coactivators and APC-C inhibitors. (nih.gov)
  • The APC/C also targets the mitotic cyclins for degradation, resulting in the inactivation of M-CDK (mitotic cyclin-dependent kinase) complexes, promoting exit from mitosis and cytokinesis Unlike the SCF, activator subunits control the APC/C. Cdc20 and Cdh1 are the two activators of particular importance to the cell cycle. (wikipedia.org)
  • Activators CDC20 and Cdh1 are of particular significance and are the most widely studied and familiar of the APC/C subunits. (wikipedia.org)
  • Their dephosphorylation is regulated depending on the sites and protein phosphatase 2A , active in mitosis , is essential to dephosphorylate the threonine residues and activate the APC/C. Consistently, most of the Cdc20 bound to the APC/C in anaphase evades phosphorylation at T79. (bvsalud.org)
  • Furthermore, we show that the 'activation domain' of Cdc20 associates with the Apc6 and Apc8 core subunits. (bvsalud.org)
  • The specificity of APC ligases is proposed to be controlled by the incorporation of specificity factors into the ligase complex, instead of substrate phosphorylation. (wikipedia.org)
  • The catalytic core of the APC/C consists of the cullin subunit Apc2 and RING H2 domain subunit Apc11. (wikipedia.org)
  • The major motifs in APC subunits include tetratricopeptide (TPR) motifs and WD40 repeats 1. (wikipedia.org)
  • A highly conserved subunit of the anaphase-promoting complex (APC-C) containing multiple 34 amino acid tetratricopeptide repeats. (nih.gov)
  • These include Apc1, the largest subunit which contains 11 tandem repeats of 35-40 amino acid sequences, and Apc2, which contains three cullin repeats of approximately 130 amino acids total. (wikipedia.org)
  • Separase then triggers the cleavage of cohesin, the protein complex that binds sister chromatids together. (wikipedia.org)
  • It consists entirely of pentameric α7 subunits expressed in the CNS, autonomic nervous system, vascular system, lymphocytes and spleen. (nih.gov)
  • It was the discovery of the APC/C (and SCF) and their key role in eukaryotic cell-cycle regulation that established the importance of ubiquitin-mediated proteolysis in cell biology. (wikipedia.org)
  • HN - 2014 MH - Allografts UI - D064591 MN - A1.941.500 MS - Tissues, cells, or organs transplanted between genetically different individuals of the same species. (nih.gov)
  • Eleven core APC subunits have been found in vertebrates versus thirteen in yeast. (wikipedia.org)
  • Studies of APC subunits are mainly conducted in yeast, and studies show that the majority of yeast APC subunits are also present in vertebrates, this suggests conservation across eukaryotes. (wikipedia.org)