CD34Role in stimulating myeloid aMurineMoleculesSTEM CELL TRANSPLANTNormal hematopoietic cellsTransplantationDifferentiation of circulatingProliferation and differentiationMyeloid progenitorsLigandPopulationsMacrophagesHematopoiesisCytokineSpecific antigenSelf-renewalMultipotentErythroidTissuesMesenchymalVivoCytokinesVitroBone marroCD45Embryonic stemMacrophageEpithelialLeukemicTumorsLineagesTyrosine kinaseInduceInnate and adaptiveDendritic cellsDistinctPathwaysLymphocytesReceptorColoniesClinicalLineageProliferativeYolk-sacRegulatorSubventricular zoneStromal cellsProliferatePeripheral blooLymphoid potentialImmunologyImmunotherapyMaturationNeuralMarrowHSPCMiceProteinDivision and differentiationChronicMarkersCD24
CD3418
- CD135 is a 130-160 kD type III tyrosine kinase receptor expressed on CD34 + hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. (biolegend.com)
- Expressed on CD34 + hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. (biolegend.com)
- CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
- CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
- In this regard, his lab has identified a novel family of hematopoietic cell surface proteins, called the CD34 family, and shown that these are essential for a number of developmentally important processes. (ubc.ca)
- This Technical Bulletin provides instructions for CRISPR-Cas9 genome editing and maintenance of human CD34 + cells, including optimized pre- and post-editing culture conditions and methods to evaluate genome editing efficiency. (stemcell.com)
- While CRISPR-Cas9 technology has been successfully applied in numerous cell lines, its application in primary human CD34 + cells has been hampered by challenges in efficient expression and delivery of CRISPR-Cas9 components. (stemcell.com)
- The ArciTect™ CRISPR-Cas9 RNP is then prepared and delivered into CD34 + cells using electroporation, and cells are plated in StemSpan™ SFEM II supplemented with StemSpan™ CD34 + Expansion Supplement post-electroporation. (stemcell.com)
- Additionally, edited CD34 + cells can be further cultured in conditions stimulating lineage-specific differentiation by combining StemSpan™ media with expansion supplements designed for the expansion and differentiation of progenitors to erythroid, myeloid, megakaryocyte, natural killer (NK), or T cell lineages. (stemcell.com)
- To identify regulators of primitive chronic myeloid leukemia (CML) cells, we performed a high-content cytokine screen using primary CD34 + CD38 low chronic phase CML cells. (haematologica.org)
- Leukaemia stem cells (LSC) are identified by high CD34 and negative CD38 expression. (ghrnet.org)
- We examined the CD45 dim CD34 + CD38 - CD133 + cells on bone marrow samples of hematologic malignancies and healthy controls using four-color flow cytometry experiments. (researchsquare.com)
- Interestingly, the CD45 dim CD34 + CD38 - CD133 + cells were highly expressed in bone marrow of patients with AML compared to that of healthy controls (HC). (researchsquare.com)
- Additionally, the high levels of the CD45 dim CD34 + CD38 - CD133 + cells in AML patients were an independently significant poor risk factor for overall survival and event free survivals. (researchsquare.com)
- Therefore, our results suggest that CD45 dim CD34 + CD38 - CD133 + cells in AML might have the potential of leukemia stem cells. (researchsquare.com)
- For examples, Rhenen et al showed that a high percentage of CD34 + CD38 - stem cells at diagnosis significantly correlated with a high minimal residual disease frequency and subsequently to relapse especially after the third course of chemotherapy in AML patients. (researchsquare.com)
- Moreover, the CD34 + CD38 - progenitor cells expressed variable amounts of the target receptor CD33, CD133 and c-kit (CD117) [ 20 ]. (researchsquare.com)
- Furthermore, it was developed using the basic platform of the CD34 + CD38 - markers, but applying more advanced antigens such as CD45 dim and CD133. (researchsquare.com)
Role in stimulating myeloid a2
- Signaling through SCF/c-Kit has an important role in stimulating myeloid and erythroid production of primitive hematopoietic progenitor cells. (southernbiotech.com)
- The ligand for c-kit (stem cell factor [SCF]) plays a key role in stimulating myeloid and erythroid cell production of primitive hematopoietic progenitors. (ox.ac.uk)
Murine7
- Combined signaling through interleukin-7 receptors and Flt3 selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells. (biolegend.com)
- Tumor necrosis factor-alpha (TNF-alpha) potently enhances in vitro macrophage production from primitive murine hematopoietic progenitor cells in combination with stem cell factor and interleukin-7: novel stimulatory role of p55 TNF receptors. (ox.ac.uk)
- TNF-alpha generally inhibits the growth of primitive murine hematopoietic progenitor cells (Lin-Scal+) in response to multiple cytokine combinations, and the p75 TNF receptor is essential in signaling such inhibition. (ox.ac.uk)
- Early in murine embryonic development, hematopoietic progenitors can be found in the para-aortic splanchnopleura region on embryonic day 7 (as depicted in Figure 1 A), but these progenitors are not LT reconstituting although they do possess lymphoid potential [ 11 - 13 ]. (biomedcentral.com)
- There is growing evidence that resident progenitor cell populations exist in murine lung tissues and differentiate into a mesenchymal cell lineage [1, 2]. (keio.ac.jp)
- Stem cell antigen-1 (Sca-1) is a cell surface glycoprotein, initially found in murine bone marrow-derived stem cell subtypes, such as hematopoietic stem cells. (keio.ac.jp)
- Some studies showed Sca-1 expression in the pulmonary vascular endothelium of adult murine lungs [3], while a subset of Sca-1-expressing cells formed vascular-like structures under specific conditions [1]. (keio.ac.jp)
Molecules6
- Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
- The GP42 in the complex is combined with the HLA II molecular molecules, and caused the virus cell fusion under the participation of GP85/GP25 and GP110 glycoprotein. (selfgrowth.com)
- EBV-infected B cells can activate NK cells to acquire CD21 molecules through synaptic transfer, and the ectopic receptor leads to the binding of EBV to NK cells. (selfgrowth.com)
- Studies have shown that EBV-infected T/NK lymphocytes often express cytotoxic molecules, such as perforin, granzyme B, and T cell intracytoplasmic antigen (TIA-1). (selfgrowth.com)
- Through gene knockout studies he has shown that these molecules act as a type of molecular "Teflon" to make cells more mobile and invasive and also facilitate chemotaxis. (ubc.ca)
- He has delineated the function of these molecules in diverse set of biological processes including: 1) gut and kidney formation, 2) vascular permeability, 3) mucosal inflammatory disease, 4) stem cell homing and migration, and 5) epithelial tumor progression. (ubc.ca)
STEM CELL TRANSPLANT1
- I have expertise in novel therapies for acute myeloid leukemia, stem cell mobilization and homing, as well as stem cell transplantation. (rochester.edu)
Normal hematopoietic cells1
- In summary, we identify myostatin propeptide as a novel positive regulator of primitive CML cells and corresponding normal hematopoietic cells. (haematologica.org)
Transplantation10
- HSCs specifically are defined by their ability for self-renewal and to reconstitute the entire hematopoietic system following transplantation. (stemcell.com)
- It is used both by hematopoietic researchers and clinical labs to assess the potency of transplantation units of cord blood (CB) and hematopoietic cellular therapy products. (stemcell.com)
- Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
- 4. Hodek M, Vávrová J, Šinkorová Z, Mokŕy J, Filip S. Hematopoietic recovery after transplantation CD117 + B220 - (LacZ + ) bone marrow cells in lethally irradiated mice. (southernbiotech.com)
- Smith and Broxmeyer, 1986), thereby making it a potential source of cells for transplantation (Bodger, 1987). (nationalacademies.org)
- 2003). After the early success of transplantation of cord blood from related donors, cord blood banks were established to provide rapidly accessible, human leukocyte antigen (HLA)-typed units predominantly for transplantation of HPCs from unrelated donors. (nationalacademies.org)
- The stromal cell requirement for androgen-mediated effects was confirmed in vivo by experiments using chimeric animals created by bone marrow transplantation in which androgen receptor expression was restricted to either the stromal or lymphoid cells of the bone marrow. (jci.org)
- Therefore, hematopoietic stem cells mobilized into a subject's peripheral blood by the method of the invention is useful as a source of donor cells in bone marrow transplantation for the treatment of a variety of disorders, including cancer, anemia, autoimmunity and immunodeficiency. (justia.com)
- In addition, in vivo serial transplantation assays, dye exclusion assays, and isolation via cell surface specific antigen profile methods are now used to identify CSCs. (biomedcentral.com)
- The established mesothelioma cell cultures are able to engraft, after pseudo orthotopic intraperitoneal transplantation, in immunodeficient mouse and maintain this ability to after serial transplantation. (biomedcentral.com)
Differentiation of circulating1
- The prenatal population of dermal macrophages is gradually replaced by the recruitment and differentiation of circulating progenitors in adulthood. (wikipedia.org)
Proliferation and differentiation3
- The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
- A variety of cytokines act synergistically with SCF to stimulate proliferation and differentiation of bone marrow progenitor cells. (southernbiotech.com)
- Differential growth factor requirement of primitive cord blood hematopoietic stem cell for self-renewal and amplification vs proliferation and differentiation. (unito.it)
Myeloid progenitors2
- It is expressed on early B lymphoid lineage cells in bone marrow, on primitive myeloid progenitors within the BM CD34+ cell population. (biolegend.com)
- Effect of interferon-gamma on HLA class II antigen expression and sensitivity to prostaglandin E1 by normal and leukemic myeloid progenitors. (unito.it)
Ligand3
- CD135, also known as FMS-like tyrosine kinase-3, FLT3, STK-1, and Flk-2, is a growth factor receptor that binds the FLT3 ligand to promote the growth and differentiation of primitive hematopoietic cells. (biolegend.com)
- Ligation of Flk-2 with Flt3 ligand regulates the growth of hematopoietic stem cells and promotes the survival of primitive hematopoietic progenitor cells with myeloid as well as B lymphoid potential. (biolegend.com)
- In the present study, the flt3-ligand (FL), which, in combination with other cytokines, has overlapping activities with SCF on myeloid cell production from uncommitted progenitors, was investigated for its ability to induce selective stroma-independent B-cell commitment from uncommitted Lin-Sca-1+ bone marrow progenitor cells. (ox.ac.uk)
Populations7
- We assessed whether cell cycle inhibitors that restrict stem cell populations in other tissues may participate in limiting neural stem cell reactivity in vivo. (rupress.org)
- Also, these cell populations directly correlated with poor survival [ 2 , 5 ]. (researchsquare.com)
- Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
- Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
- At least two populations of tissue macrophages exist: tissue-resident cells that are embryonically derived and a monocyte-derived population. (musculoskeletalkey.com)
- The administration of nonlethal irradiation to induce death of hematopoietic cells and their precursors followed by administration of donor bone marrow (radiation chimeras) revealed populations of macrophages, including synovial macrophages, Langerhans cells, and microglia, that were resistant to irradiation and remained host origin, whereas the monocyte population was derived from donor hematopoietic cells. (musculoskeletalkey.com)
- Recent studies have isolated distinct cell populations from infantile hemangiomas that display properties equivalent to aberrant progenitor cells, suggesting that, in addition to malignant tumors, benign tumors may also contain a stem cell-like component. (medscape.com)
Macrophages20
- Dermal macrophages are native immune cells mainly located in the dermis of the skin. (wikipedia.org)
- They are also a type of antigen-presenting cells (APCs) that can mediate the infiltration of immune cells during an immune response, suggesting dermal macrophages' influence on both the innate and adaptive immune systems. (wikipedia.org)
- Dermal macrophages are either from embryonic progenitors or circulating progenitors. (wikipedia.org)
- Numerous dermal macrophages are present in the skin at birth due to the infiltration of yolk-sac derived macrophages and haematopoietic stem cell (HSC)-derived monocytes. (wikipedia.org)
- Prenatal dermal macrophages are not to be confused with Langerhans cells as they uniquely express CD14 and FXIIIa and lack CD1a. (wikipedia.org)
- The generation of dermal macrophages results from primitive haematopoiesis or definitive haematopoiesis. (wikipedia.org)
- Primitive haematopoiesis allows the generation of yolk-sac derived macrophages and subsequent release into the foetal bloodstream for tissue infiltration and colonisation. (wikipedia.org)
- The generation of dermal macrophages is highlighted by the expression of cell markers such as CD64, MERTK and the downregulation of CCR2. (wikipedia.org)
- Dermal macrophages can phagocytose and digest foreign substances similar to other cell types in the mononuclear phagocyte system. (wikipedia.org)
- Dermal macrophages, Langerhans cells and dendritic cells are the main types of antigen-presenting cells (APCs) in the skin. (wikipedia.org)
- Although CD4 T cells are the main targets of HIV infection, macrophages also become infected and resist the cytopathic effects of infection, contributing potentially to HIV reservoir persistence. (nih.gov)
- It is now known that type A cells were synovial macrophages, type B were synovial fibroblasts, and type C were an undetermined population. (musculoskeletalkey.com)
- Further studies in the 1980s and early 1990s refined the classification of synovial macrophages with the use of immunohistochemistry and known antibodies to antigen-presenting cells. (musculoskeletalkey.com)
- Taken together, these fundamental studies provided the basis that the synovial macrophages were derived from hematopoietic progenitors and represented a heterogeneous population in both normal and inflamed synovium. (musculoskeletalkey.com)
- These studies determined that adult monocytes develop as precursors in the bone marrow and then enter the circulation to replenish macrophages in tissue through the use of radiolabeled monocyte progenitors. (musculoskeletalkey.com)
- Initial studies suggested that, in adults, a common monocyte/dendritic cell (MDP) progenitor in the bone marrow leads to the development of monocytes, macrophages, and dendritic cells. (musculoskeletalkey.com)
- Moreover, parabiotic mice that share the same circulation showed that only a subset of macrophages such as heart, gut, and dermis exhibited a mixed population, whereas Langerhans cells, microglia, and alveolar macrophages were exclusively derived from the parent mouse. (musculoskeletalkey.com)
- In multicellular organisms, and more particularly in animals with a well-developed immune system, phagocytosis is mostly performed by specialized, professional phagocytes: macrophages, dendritic cells (DCs), and neutrophils. (frontiersin.org)
- Cell biology of phagocytosis has been mainly established on bone marrow-derived tissue macrophages. (frontiersin.org)
- Particularly, yolk sac-derived macrophages including microglia may possibly have different tasks since they are confronted with different target structures, mainly apoptotic cells during developmental tissue remodeling, while bone marrow-derived macrophages have a higher chance to be confronted with pathogens, mainly during defense against invading microbes. (frontiersin.org)
Hematopoiesis6
- Tumor necrosis factor-alpha (TNF-alpha) is a bifunctional regulator of hematopoiesis, and its cellular responses are mediated by two distinct cell surface receptors. (ox.ac.uk)
- Mouse hematopoiesis was thought to develop initially in the yolk sac, where primitive hematopoietic development occurs [ 4 ]. (biomedcentral.com)
- Their results indicated that yolk sac progenitors before the onset of circulation did not have LT reconstitution potential while progenitors isolated from the AGM did, which indicates that primitive and definitive hematopoiesis may occur at distinct anatomical locations [ 10 ]. (biomedcentral.com)
- The ability to genetically manipulate hematopoietic stem and progenitor cells (HSPCs) in particular has significantly advanced our understanding of the mechanisms that regulate hematopoiesis and is contributing to the development of novel cellular therapies. (stemcell.com)
- Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
- In approximately 15% of patients, however, myelofibrosis is accompanied by significant extramedullary hematopoiesis, hepatosplenomegaly, and transfusion-dependent anemia, which are manifestations of stem cell failure. (basicmedicalkey.com)
Cytokine6
- 2 1 There is growing evidence to suggest that primitive CML cells affect the bone marrow (BM) niche, contributing to deregulated cytokine levels. (haematologica.org)
- Cytokines are essential for the function and maintenance of cells, and altered cytokine levels influence not only leukemic cells, but also the normal HSC within the BM. (haematologica.org)
- Effects of hematopoietic stem cell adhesion on marrow stromal cell cytokine p. (rochester.edu)
- Cytokine-induced killer (CIK) cells as feasible and effective adoptive immunotherapy for the treatment of solid tumors. (unito.it)
- Cytokine-induced killer cells eradicate bone and soft-tissue sarcomas. (unito.it)
- Many of the clinical complications of PV relate directly to the increase in blood viscosity associated with red cell mass elevation and indirectly to the increased turnover of red cells, leukocytes, and platelets with the attendant increase in uric acid and cytokine production. (basicmedicalkey.com)
Specific antigen2
- Elevated levels of anti-EBV-specific antigen IgA were found in mucosal secretions of NPC patients, and this EBV-IgA-SC-mediated endocytosis may represent a physiological pathway for EBV to invade nasopharyngeal epithelial cells in vivo. (selfgrowth.com)
- Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. (thermofisher.com)
Self-renewal4
- True stem cells are both pluripotent-that is they can give rise to all cell types-and capable of self-renewal. (justia.com)
- Acute myeloid leukemia (AML) is generally regarded as a stem cell disease, and it originates from a hierarchy of leukemic stem cell classes that differ in self-renewal capacity [ 1 , 2 ]. (researchsquare.com)
- Altogether our FACS (fluorescence-activated cell sorter) analyses reveal that the neonatal subventricular zone is far more heterogeneous than previously suspected and our studies provide new insights into the signals and mechanisms that regulate their self-renewal and proliferation. (karger.com)
- To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)
Multipotent5
- Multiple cytokines can synergize to stimulate the in vitro proliferation and exclusive myeloid differentiation of multipotent bone marrow progenitor cells. (ox.ac.uk)
- B lood cell differentiation begins with multipotent hematopoietic progenitor cells (HPCs), which are located in the marrow spaces of the bone. (nationalacademies.org)
- These committed progenitor cells are difficult to discern from the original multipotent cells but can be cultured to form colonies of specific types of blood cells (Guyton and Hall, 2000). (nationalacademies.org)
- Umbilical cord blood is a rich source of these committed progenitor cells and, presumably, multipotent HPCs (Knudtzon, 1974). (nationalacademies.org)
- FIGURE 2-1 Formation of the multiple peripheral blood cells from multipotent hematopoietic stem cells. (nationalacademies.org)
Erythroid1
- Phenotyping showed that greater than 99% of the cells produced were of the B-cell lineage, in that they expressed B220, but not cell surface markers specific for myeloid, erythroid, or T-cell lineages. (ox.ac.uk)
Tissues9
- CD140b is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. (thermofisher.com)
- Results: In this study, we found high expression of the HOXD9 gene transcript in glioma cell lines and human glioma tissues by quantitative real-time PCR. (123dok.net)
- The subject's blood can be processed and used to repopulate the destroyed lymphohematopoietic system of a recipient and may in the future be utilized to repair a variety of non-hematopoietic tissues. (justia.com)
- In humans, at least two subsets of DC are known in the blood, myeloid DC (also known as interstitial or dermal DC), and plasmacytoid DC (pDC) and Langerhans cells (LC) in the tissues [ 3 ]. (biomedcentral.com)
- Unlike some other tissues, it has not been possible to identify or purify neural stem cells directly from the tissue. (elifesciences.org)
- Cells referred to as MSCs originating from various tissues are now used in clinical trials. (biomedcentral.com)
- Over the last years, dentistry has been exploring the potential application of stem cells from different origins in the regeneration of oral tissues that were lost or damaged by disease or trauma 1 . (bvsalud.org)
- Antigenicity for each protein was quantified based on staining intensity and percentage of tissue positive for each antigen, and subsequently compared to data obtained from two control tissue sets: 10 vascular tissues and a panel of 58 various malignant sarcomas. (medscape.com)
- Identification and expansion of cancer stem cells in tumor tissues and peripheral blood derived from gastric adenocarcinoma patients. (celljournal.org)
Mesenchymal4
- These growth factors are mitogens for cells of mesenchymal origin. (thermofisher.com)
- Furthermore, we show expression of MSTN by CML mesenchymal stromal cells, and that myostatin propeptide has a direct and instant effect on CML cells, independent of myostatin, by demonstrating binding of myostatin propeptide to the cell surface and increased phosphorylation of STAT5 and SMAD2/3. (haematologica.org)
- Because of their multi/pluripotency and immunosuppressive properties, mesenchymal stem/stromal cells (MSCs) are important tools for treating immune disorders and for tissue repair. (biomedcentral.com)
- Producing cells according to Good Manufacturing Practices (GMPs) is a global challenge for the production of all cells for use in humans, specifically mesenchymal stem/stroma cells (MSCs), one of the most promising tools in cellular therapy and regenerative medicine. (biomedcentral.com)
Vivo7
- Neural precursor cells from adults have exceptional proliferative and differentiative capability in vitro yet respond minimally to in vivo brain injury due to constraining mechanisms that are poorly defined. (rupress.org)
- Pre-GEPCOT cells could not form neurospheres but expressed the stem cell markers Slc1a3-CreER T , GFAP-CreER T2 , Sox2 CreERT2 , and Gli1 CreERT2 and were long-lived in vivo. (elifesciences.org)
- Different growth factor requirements for the ex vivo amplification of transplantable human cord blood cells in a NOD/SCID mouse model. (unito.it)
- These cell cultures may provide a valuable in vitro and in vivo model to investigate mesothelioma biology. (biomedcentral.com)
- Critically, in both diseases, upstream immunological processes have been poorly characterised ex vivo , particularly with respect to the site and nature of antigen presentation. (biomedcentral.com)
- This remark is certainly a stunning point in favor of a significant Refametinib contribution to growth development control by FSP-specific Testosterone levels cells in vivo , producing those peptides extremely interesting applicants for the advancement of targeted vaccination strategies. (immune-source.com)
- Ex vivo Hsp70-activated nk cells in combination with pd-1 inhibition significantly increase overall survival in preclinical models of glioblastoma and lung cancer. (celljournal.org)
Cytokines3
- They can acquire an M1 or M2 phenotype to promote or suppress an inflammatory response, thereby influencing other cells' activity via the production of pro-inflammatory or anti-inflammatory cytokines. (wikipedia.org)
- Aberrantly expressed cytokines in the bone marrow (BM) niche are increasingly recognized as critical mediators of survival and expansion of leukemic stem cells. (haematologica.org)
- Out of the 313 unique human cytokines evaluated, 11 were found to expand cell numbers ≥2-fold in a 7-day culture. (haematologica.org)
Vitro3
- Progenitor cell function may be measured post-modification by in vitro CFU assays. (stemcell.com)
- Effect of acidic and basic isoferritins on in vitro growth of human granulocyte-monocyte progenitors. (unito.it)
- The primary cell cultures, obtained from four human pleural mesotheliomas, were expanded in vitro in a low serum proliferation-permissive medium and the expression of different markers as well as the tumorigenicity in immunodeficient mice was evaluated. (biomedcentral.com)
Bone marro9
- C57BL/6 mouse bone marrow cells were stained with Rat Anti-Mouse CD117-BIOT (SB Cat. (southernbiotech.com)
- 50 cells), whereas the combined stimulation with FL and IL-7 resulted in clonal growth of 10% of Lin-Sca-1+ bone marrow cells. (ox.ac.uk)
- Multicolor flow cytometric analysis of Ly-76 (TER-119) expression on viable Mouse bone marrow cells. (bdbiosciences.com)
- BALB/c Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. (bdbiosciences.com)
- The bivariate pseudocolor density plot showing the correlated expression of Ly-76 (TER-119) [or Ig Isotype control staining] versus CD45 was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) bone marrow cells. (bdbiosciences.com)
- Stem cells represent a very small percentage of bone marrow cells, and are normally quiescent. (justia.com)
- In this study, we focused on measuring LSCs easily in the bone marrow cells from AML patients by developing a four-color flow cytometric analysis. (researchsquare.com)
- Investigation of bone marrow cells showed that these became apoptotic within seven days after poly injection. (biomedcentral.com)
- Furthermore, infusion of normal bone marrow cells rescued mice from death induced by loss of THOC5/Fms interacting protein. (biomedcentral.com)
CD452
- The cells were then stained with APC Rat Anti-Mouse CD45 antibody (Cat. (bdbiosciences.com)
- We prospectively identified, and isolated by flow cytometry, adult mouse lateral ventricle subventricular zone (SVZ) NICs as Glast mid EGFR high PlexinB2 high CD24 −/low O4/PSA-NCAM −/low Ter119/CD45 − (GEPCOT) cells. (elifesciences.org)
Embryonic stem1
- We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)
Macrophage3
- Then, we will discuss several principles that govern macrophage phagocytosis of apoptotic cells (efferocytosis), including the existence of redundant recognition mechanisms ("find-me" and "eat-me") that lead to a tight coupling between apoptosis and phagocytosis. (frontiersin.org)
- Because it is such a diverse population of cells, a fundamental question is whether they have a common precursor or whether each macrophage population develops from a different precursor. (frontiersin.org)
- However, they have a different origin and are a unique macrophage cell type in the adult organism. (frontiersin.org)
Epithelial15
- At present, tumors related to EBV infections include epithelial tumors such as nasopharyngealcarcinoma(NPC), gastric carcinoma (GC), lymphatic hematopoietic malignant tumors such as Hodgkin's Lymphoma (HL) and Non-Hodgkin Lymphoma (NHL), BL, pyropogy-related lymphoma (PAL) and NK/T cell lymphoma and other rare tumors (such as EBV-related smooth fibroids, neuromuscular endocrine cancer). (selfgrowth.com)
- Oral mucosal epithelial cells are the first threshold for their invasive host cells. (selfgrowth.com)
- EBV can also be transmitted from EBV-infected B cells or epithelial cells to T/NK lymphocytes by cell-to-cell infection. (selfgrowth.com)
- NK cells, CD8+ T cells, and γδ T cells observed in EBV-associated T/NK lymphocyte tumors are typical of EBV-infected cells and belong to the type of killer cells that attempt to kill EBV-infected B cells or epithelial cells. (selfgrowth.com)
- Epithelial cells do not express CR2, and the mechanism of how EBV invades and releases from epithelial cells is not yet clear. (selfgrowth.com)
- 3) After primary infection, EBV spreads directly across the lateral membrane to adjacent epithelial cells. (selfgrowth.com)
- The anti-EBV antigen polymer IgA can mediate the invasion of EBV into pharyngeal epithelial cells through endocytosis, and EBV bound to IgA can invade pharyngeal epithelial cells through endocytosis mediated by secretory corpuscle (SC). (selfgrowth.com)
- EBV infection of glandular epithelial cells can cause gastric cancer and bile duct cancer, and the mechanism is unclear. (selfgrowth.com)
- Description: The G8.8 monoclonal antibody reacts with the 40 kDa mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40 (epithelial glycoprotein 40), 17-1A antigen, TACSTD1 (tumor-associated calcium signal transducer 1), and CD326. (thermofisher.com)
- The immunogen used to generate the G8.8 antibody was the TE-71 thymic epithelial cell line. (thermofisher.com)
- CD326 is expressed on the majority of epithelial cells, and is considered a pan-carcinoma antigen. (thermofisher.com)
- Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. (thermofisher.com)
- Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. (thermofisher.com)
- On the other hand, clear cell acanthoma is a rare, benign epithelial cutaneous tumor. (medscape.com)
- Clear cell acanthoma usually stains positively for epithelial membrane antigen and negatively for carcinoembryonic antigen. (medscape.com)
Leukemic1
- A pro-inflammatory environment is thought to provide a selective advantage for the leukemic stem cells (LSC). (haematologica.org)
Tumors14
- The histologic spectrum of AT/RT overlaps significantly with other forms of CNS neoplasia, including medulloblastomas, germ cell tumors, sarcomas, and choroid plexus carcinomas. (medscape.com)
- The term "malignant rhabdoid tumor" was first used to describe rare pediatric renal tumors that contained tumor cells with vesicular nuclei, prominent nucleoli, and cytoplasmic inclusion-like structures composed of whorls of intermediate filaments. (medscape.com)
- A rare group of embryonal CNS neoplasms that arise outside of the cerebellum and pineal gland were previously called "central nervous system primitive neuroectodermal tumors" (CNS PNETs). (medscape.com)
- Such tumors may arise in the cerebral hemispheres, brainstem, or spinal cord, and they are composed of immature neuroepithelial cells that may express neuronal and glial antigens. (medscape.com)
- The researchers found that even when radiation kills half of the tumor cells treated, the surviving cells which are resistant to treatment, known as induced breast cancer stem cells (iBCSCs), were up to 30 times more likely to form tumors than the nonirradiated breast cancer cells. (mexicancancerclinics.com)
- Tumors are actually highly organized assemblages of cells, which are surprisingly well-coordinated for cells that are supposed to be the result of strictly random mutation. (mexicancancerclinics.com)
- Because tumors are not simply the result of one or more mutated cells "going rogue" and producing exact clones of itself (multi-mutational and clonal hypotheses), but are a diverse group of cells having radically different phenotypal characteristics, chemotherapy and radiation will affect each cell type differently. (mexicancancerclinics.com)
- Tumors are composed of a wide range of cells, many of which are entirely benign. (mexicancancerclinics.com)
- In this regard, tumor cell lines development has been an important tool to study the biological properties of many tumors. (biomedcentral.com)
- As with all tumors, whether benign or malignant, the paradigm of comprehension depends on identifying the cell or cell layer of origin. (medscape.com)
- Skin malignancies, Merkel cell carcinoma and rare appendageal tumors. (medscape.com)
- When evaluating with microsatellite steady tumors, there is normally some proof for Kitty least partialC immunological development control in MSI malignancies, like (I) the thick regional lymphocytic infiltration (Crohn's-like lymphocytic response), (II) the elevated apoptotic growth cell amount, and (3) the low amount of isolated metastases that (4) network marketing leads to an improved general individual success [1]C[3]. (immune-source.com)
- The "stem cell theory of cancer" states that a subpopulation of cells with stem cell-like properties plays a central role in the formation, sustainment, spread, and drug resistant characteristics of malignant tumors. (medscape.com)
- These findings provide supporting evidence that enrichment for proteins involved in pluripotency is not restricted solely to malignant tumors as is suggested by the "stem cell theory of cancer", but additionally extends to common benign vascular tumors such as hemangiomas. (medscape.com)
Lineages4
- The future direction of the study of hematopoietic stem cells will probably make use of pluripotent stem cells to yield specific immune cell lineages and eventual clinical applications. (biomedcentral.com)
- These more advanced hematopoietic progenitors isolated from the aorta-gonad-mesonephros (AGM) region at embryonic day 9.5 to 10 can give rise to lymphoid lineages and are LT reconstituting definitive HSCs, but since this time point is after the establishment of the circulatory system in the embryo their source was difficult to determine [ 13 ]. (biomedcentral.com)
- Objective: To establish cultures of cells from the pulp of permanent teeth by the explant method assessing parameters usually presented by stem cells, such as the expression of certain markers and the differentiation ability into osteogenic, adipogenic, and chondrogenic lineages. (bvsalud.org)
- Similarly, the cells showed differentiation ability into all three lineages of cells tested. (bvsalud.org)
Tyrosine kinase3
- It was reported that the receptor tyrosine kinase Flt3 is required for dendritic cell development. (biolegend.com)
- PDGFRb is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. (thermofisher.com)
- 1 The BCR-ABL1 fusion protein is a constitutively active tyrosine kinase and triggers a cascade of aberrant downstream signaling pathways leading to clonal outgrowth of CML cells and subsequent disease manifestation. (haematologica.org)
Induce2
- Gene modified dendritic cells (DC) are able to modulate DC functions and induce therapeutic immunity or tolerance in an antigen-specific manner. (biomedcentral.com)
- Periodic phlebotomies thereafter serve to maintain the red cell mass within the normal range and to induce a state of iron deficiency that prevents an accelerated reexpansion of the red cell mass. (basicmedicalkey.com)
Innate and adaptive1
- Dendritic cells (DCs) comprise heterogeneous subsets of professional antigen-presenting cells, linking innate and adaptive immunity. (biomedcentral.com)
Dendritic cells1
- However, their antigen-presenting ability is relatively lower than dendritic cells (DCs) and Langerhans cells (LCs) in the skin. (wikipedia.org)
Distinct2
- Distinct anatomical regions of primitive and definitive hematopoietic development in both the mouse and human. (biomedcentral.com)
- Therefore, p21 is an intrinsic suppressor to neural regeneration after brain injury and may serve as a common molecular regulator restricting proliferation among stem cell pools from distinct tissue types. (rupress.org)
Pathways2
- MiRNA-driven pathways are fundamental for the maintenance and proper function of cell stemness in embryonic stem cells. (biomedcentral.com)
- In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)
Lymphocytes4
- EBV shows obvious tendency to B lymphocytes, which is easy to infect B cells and convert initial B cells into proliferative lymphocytes. (selfgrowth.com)
- Studies have shown that both primitive T lymphocytes and lymphoid progenitor cells can express CD21, and EBV can also infect primitive T cells and lymphoid progenitor cells by attaching to T lymphocytes through CD21. (selfgrowth.com)
- The double infection of T lymphocytes and NK cells in patients with chronic active EBV infection (CAEBV) has been reported in the literature, further supporting that EBV may infect the common progenitor cells of T cells and NK cells. (selfgrowth.com)
- Recent studies, however, suggest that MDPs may also develop into other types of hematopoietic cells, such as lymphocytes. (musculoskeletalkey.com)
Receptor6
- In the present study we show the reverse phenomenon in that TNF-alpha on the same progenitor cell population in combination with stem cell factor (SCF) and interleukin-7 (IL-7) through the p55 TNF receptor can recruit additional progenitors to proliferate. (ox.ac.uk)
- The CD117 (c-Kit) antigen is the cell-surface receptor for stem cell factor (SCF). (southernbiotech.com)
- Viral glycoproteins including gp350, gHgL, gB and gp42 mediate the preferential binding of EBV to B cells by interacting with the complement receptor CR2 (CD21) on the surface of B cells, and then the envelope glycoprotein gp42 and gp85/gp25 form a fusion protein triple molecule Complex. (selfgrowth.com)
- They are capable of building their own blood supply (angiogenesis), are able to defend themselves by silencing cancer-suppression genes, secreting corrosive enzymes to move freely throughout the body, alter their metabolism to live in low oxygen and acidic environments, and know how to remove their own surface-receptor proteins to escape detection by white blood cells. (mexicancancerclinics.com)
- CD326 mediates calcium-independent, homophilic, cell-cell adhesion and may function as a growth factor receptor. (thermofisher.com)
- Phagocytosis is a term coined from the Greek to describe the receptor-mediated engulfment and degradation of dead cells and microbes. (frontiersin.org)
Colonies7
- TNF-alpha increased the number of responding progenitors, as well as the size of the colonies formed. (ox.ac.uk)
- Colonies derived from different types of progenitor cells are classified and counted based on morphological and phenotypic criteria. (stemcell.com)
- Consequently, neural stem and progenitor cells have usually been studied retrospectively, based on their ability to form colonies in laboratory cell cultures. (elifesciences.org)
- When cells from the subventricular zone are cultured in a way that allows the cells to freely float around (rather than growing on a surface), a few percent form spherical colonies called neurospheres. (elifesciences.org)
- term pre-GEPCOT cells (based on an acronym of the markers used to isolate the cells), were long-lived and quiescent, but they lacked the ability to form colonies in culture. (elifesciences.org)
- These results demonstrate that the cells that form neurospheres in culture are not stem cells at all, and that real stem cells are not able to form colonies under existing culture conditions. (elifesciences.org)
- MSCs with a mesodermic origin were first described by Alexander Friedenstein and colleagues in the 1960s and 1970s[ 1 ] as non-hematopoietic bone marrow (BM) cells that adhered to plastic and that could develop into colonies with a fibroblastic appearance. (biomedcentral.com)
Clinical3
- Cell therapy: filling the gap between basic science and clinical trials October 15-17, 2001, Rome, Italy. (unito.it)
- The biological properties and clinical potential of stem cells elicit that are generated must not be unduly sensitive to small fluctu- continued scientific, commercial, and public interest. (lu.se)
- Typically, the clinical presentation of clear cell acanthoma is a solitary nodule in the lower extremities. (medscape.com)
Lineage8
- Also making up a part of the hematopoietic system are short-term repopulating progenitor cells, which give rise to lineage-specific cell types. (stemcell.com)
- Interestingly, although all FL + IL-7-responsive Lin-Sca-1+ progenitor cells and the resulting pro-B cells expressed c-kit, FL + IL-7 was much more potent (62-fold) than SCF + IL-7 in stimulating production of cells of the B-cell lineage. (ox.ac.uk)
- Replating studies showed that FL + IL-7-responsive Lin-Sca-1+ progenitors were not committed to the B-cell lineage, because after 2 days of incubation in FL + IL-7, 80% of the progenitors retained a myeloid potential. (ox.ac.uk)
- As much as 27% of the FL + IL-7-responsive progenitors remained uncommitted after 7 days of incubation, but all had committed to the B-cell lineage after 10 days of incubation in FL + IL-7. (ox.ac.uk)
- Acute myeloid leukemia (AML) results from the over-proliferation of progenitor cells of the myeloid lineage in the bone marrow. (ghrnet.org)
- The nature of the stem cell substates and their relationship to commitment to differ- entiate and lineage selection can be elucidated in terms of a landscape picture in which stable states can be defined mathematically as attractors. (lu.se)
- This requirement strongly limits the number of solutions or entiation and lineage-specification, programmed cell death, and ``states'' for the system. (lu.se)
- 2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)
Proliferative2
- When stimulated to divide, they give rise to more committed, differentiated daughter cells with less proliferative potential, called "early progenitor" cells. (justia.com)
- The neurosphere-forming cells, named GEPCOT cells, were short-lived and highly proliferative in the brain. (elifesciences.org)
Yolk-sac1
- Cumano and colleagues' approach was to culture explants containing hematopoietic progenitors isolated from the yolk sac and the embryo proper before the onset of circulation, and to determine their LT reconstitution potential [ 10 ]. (biomedcentral.com)
Regulator1
- In contrast, TGF-beta 1, another bifunctional regulator of hematopoietic progenitor cell growth, completely blocked SCF plus IL-7-induced proliferation. (ox.ac.uk)
Subventricular zone3
- Although steady-state conditions revealed no increase in primitive cell proliferation in p21-null mice, a significantly larger fraction of quiescent neural precursors was activated in the hippocampus and subventricular zone after brain ischemia. (rupress.org)
- A region of the brain called the subventricular zone contains both neural stem cells and neural progenitor cells, and is one of only two regions of the brain where neural stem cells are found in adult mammals. (elifesciences.org)
- have used a technique called flow cytometry to identify and isolate neural stem cells and neurosphere-forming cells directly from the subventricular zone. (elifesciences.org)
Stromal cells6
- Immature B cells from normal mouse bone marrow were not responsive to the suppressive effect of androgens unless they were cocultured with marrow stromal cells or with supernatants from androgen-treated stromal cells, suggesting that the androgen effects are exerted through marrow stromal elements by production of a diffusible mediator. (jci.org)
- Further experiments revealed that bone marrow stromal cells produced TGF-β in response to dihydrotestosterone (DHT), and neutralization of TGF-β in the DHT-treated stromal cells reversed the suppressive effects. (jci.org)
- Androgens only affected B cell development in chimeric mice with androgen-sensitive stromal cells. (jci.org)
- These experiments suggest that effects of androgens on developing B cells are mediated through androgen receptors in bone marrow stromal cells. (jci.org)
- Background: Stromal cells are a functionally important component of human carcinomas. (immune-source.com)
- Western blot analysis showed that stromal cells of lung tumours were characterised by a statistically considerably lower phrase level of the p16 proteins as likened with that in regular lung stromal cells. (immune-source.com)
Proliferate1
- Neural stem cells can also give rise to neural progenitor cells, which proliferate rapidly during their short lives and then 'differentiate' into neurons or glia. (elifesciences.org)
Peripheral bloo1
- These primitive cells undergo division and differentiation to form the various peripheral blood cells. (nationalacademies.org)
Lymphoid potential1
- Overall, they demonstrated that the definitive HSC differs from the primitive HSC by lymphoid potential as well as in their ability to achieve LT reconstitution following transfer into adult recipients. (biomedcentral.com)
Immunology2
- Phagocytosis of invading microorganisms by immune cells was first discovered by the father of cellular immunology Ilya Metchnikoff in 1882, for which he was awarded the Nobel Prize. (frontiersin.org)
- Using the traditional reverse-immunology strategy, Testosterone levels cells from healthful HLA-A0201+ contributor are triggered by artificial FSPs. (immune-source.com)
Immunotherapy2
- The antigen is being used as a target for immunotherapy treatment of human carcinomas. (thermofisher.com)
- Conclusion: Altogether, we found that heat-induced upregulated expression of gp96 in CSCs enriched mammospheres from breast tumor tissue might be used as a complementary procedure to generate more immunogenic antigens in immunotherapy settings. (celljournal.org)
Maturation4
- 3. Åbrink M, Grujic M, Pejler G. Serglycin is essential for maturation of mast cell secretory granule. (southernbiotech.com)
- There he worked with Dr. Max D. Cooper (Howard Hughes Medical Institute, National Academy of Sciences) and his research focused on cell surface proteins expressed by preB cells that regulate B cell maturation and homing. (ubc.ca)
- There his work focused on transcriptional control of hematopoietic stem cell maturation and cell fate. (ubc.ca)
- We found that efficient gene transfer into pDC can be achieved with VSVG-pseudotyped lentiviral vectors (LV) under the control of phoshoglycerate kinase (PGK) and elongation factor-1 (EF1α) promoters (28% to 90% of E-GFP + cells, respectively) in the absence of phenotypic and functional maturation. (biomedcentral.com)
Neural8
- Finally, we cultured human glioma cancer stem cells (GCSCs) from patient specimens found with high expression of HOXD9 in GCSCs compared with normal astrocyte cells and neural stem/progenitor cells (NSPCs). (123dok.net)
- The cyclin-dependent kinase inhibitor, p21 cip1/waf1 (p21), maintains hematopoietic stem cell quiescence, and we evaluated its role in the regenerative response of neural tissue after ischemic injury using the mice deficient in p21. (rupress.org)
- Neurosphere formation is commonly used as a surrogate for neural stem cell (NSC) function but the relationship between neurosphere-initiating cells (NICs) and NSCs remains unclear. (elifesciences.org)
- Neurons that arise in the adult nervous system originate from neural stem cells and neural progenitor cells. (elifesciences.org)
- Neural stem cells have long lives, much of which they spend in a quiescent state. (elifesciences.org)
- However, the exact relationship between neural stem cells and neurosphere-forming cells has been uncertain. (elifesciences.org)
- The neural stem cells, which Mich, Signer et al. (elifesciences.org)
- Here we define a 4-color flow cytometry panel using CD133, LeX, CD140a, NG2 to define a neural stem cell (NSC) as well as 4 classes of multipotential progenitors and 3 classes of bipotential progenitors, several of which have not been described previously. (karger.com)
Marrow17
- NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
- Splenectomy influences homing of transplanted stem cells in bone marrow-ablated mice. (southernbiotech.com)
- Castration of normal male mice induces expansion of the bone marrow B cell population, an effect that can be reversed by androgen replacement. (jci.org)
- Several lines of evidence support the hypothesis that gonadal steroids exert effects on the development of B cells in bone marrow. (jci.org)
- Androgens also exert effects on B cell development in bone marrow. (jci.org)
- Improved methods and pharmaceutical compositions are provided herein for mobilizing hematopoietic progenitor cells from bone marrow into peripheral blood, comprising the administration of an effective amount of an inhibitor of GTPases, such as Rac1 and Rac2 alone or in combination. (justia.com)
- The present invention relates generally to improved methods and pharmaceutical compositions for mobilizing hematopoietic stem and progenitor cell from bone marrow into peripheral blood by administration of at least one inhibitor of a GTPase, such as Rac1 and/or Rac2 GTPase. (justia.com)
- These therapies decrease a patient's white blood cell counts, suppress bone marrow hematopoietic activity, and increase the patient's risk of infection and/or hemorrhage. (justia.com)
- Current treatments to manage the problems that result from prolonged bone marrow suppression include the reinfusion of a patient's own previously harvested hematopoietic stem and progenitor cells. (justia.com)
- In such procedures, patients undergo successive treatments with cell mobilization agents to cause mobilization of hematopoietic progenitor cells from the bone marrow to the peripheral circulation for harvesting. (justia.com)
- After harvesting, the patient is given high dose chemotherapy or radiotherapy and the bone marrow function is reconstituted by infusion of the cells harvested earlier. (justia.com)
- The use of high-dosage chemotherapy or radiotherapy for bone marrow ablation requires subsequent incorporation of hematopoietic stem cells into the patient, in which case prior harvesting of such cells is required. (justia.com)
- The success of treatment crucially depends on the mobilization of the bone marrow stem cells, the subsequent return of which permits the patient to achieve reconstitution of a functioning hematopoietic system. (justia.com)
- Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. (lecturio.com)
- Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. (lecturio.com)
- Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells. (lecturio.com)
- Committed myeloid progenitor cells and cells with long term reconstituting potential were lost from bone marrow within four days after poly injection. (biomedcentral.com)
HSPC1
- Additional markers can be used to distinguish HSPC subsets within the CD34+ population and isolate HSPCs with different engraftment abilities and capacities to expand or generate mature blood cells in culture. (stemcell.com)
Mice3
- Further studies in mice by Cumano and colleagues sought to address the issue of an anatomical origin of the long-term (LT) reconstituting hematopoietic stem cell (HSC) found in adult animals [ 10 ]. (biomedcentral.com)
- T cells in cryptopatch aggregates share TCR γ variable region junctional sequences with γδ T cells in the small intestinal epithelium of mice. (southernbiotech.com)
- These mice express tamoxifen-induced Cre recombinase (Mer-cre-Mer) that enzymatically removes a stop codon flanked by lox sequences (floxed) on a reporter gene such as green fluorescent protein or yellow fluorescent protein, thus resulting in traceable fluorescence on the cells of interest. (musculoskeletalkey.com)
Protein7
- What started as a series of microscopic observations in different animal model systems has since evolved into studies of gene expression and regulation, and subsequent protein-protein interactions, cell surface protein expression profiling, and functional mapping of cell fates. (biomedcentral.com)
- EBV initially enters the body through the oropharyngeal mucosa and infects B cells through the binding of the viral envelope protein gp350 to CD21 on the surface of B cells. (selfgrowth.com)
- Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm caused by an acquired 9;22-chromosomal translocation in a hematopoietic stem cell (HSC) resulting in the expression of the BCR-ABL1 fusion protein. (haematologica.org)
- Conditional deletion of the Bmi-1 polycomb protein depleted pre-GEPCOT and GEPCOT cells, though pre-GEPCOT cells were more dependent upon Bmi-1 for Cdkn2a ( p16 Ink4a ) repression. (elifesciences.org)
- From development to aging and disease, the brain parenchyma is under the constant threat of debris accumulation, in the form of dead cells and protein aggregates. (frontiersin.org)
- Furthermore, mechanistically depletion of THOC5/Fms interacting protein causes the down-regulation of its direct interacting partner, THOC1 which may contribute to altered THO complex function and cell death. (biomedcentral.com)
- The Refametinib ending changed necessary protein typically additionally absence regular efficiency but, they constitute neo-epitopes, when provided in the circumstance of MHC elements at the growth cells' surface area. (immune-source.com)
Division and differentiation2
- Sequential rounds of division and differentiation give rise to an enormous amplification of cell numbers, necessary for the production of mature blood cells. (justia.com)
- This process of division and differentiation is subject to regulation at many levels to control cell production. (justia.com)
Chronic2
- Effect of lithium on normal and chronic granulocytic leukemia colony forming cells (CFU-GM). (unito.it)
- The inflammatory arthritides, including rheumatoid arthritis (RA) and psoriatic arthritis (PsA), comprise autoimmune disorders characterised by chronic joint inflammation, immune cell infiltration to the synovium, fibroblast-like synoviocyte expansion and destruction of cartilage and bone. (biomedcentral.com)
Markers6
- (B) Subset of cell surface markers used in the characterization of this developmental process. (biomedcentral.com)
- Cells were tested for the expression of surface markers and for ALDH1 enzyme activity, by flow cytometry. (bvsalud.org)
- The isolated cells may be considered stem cells, based on the current criteria for their characterization, such as plastic adherence, expression of certain markers, and the absence of others, as well as multi-differentiation potential, which showed to be promising for the application in tissue regeneration. (bvsalud.org)
- Our cell cultures were strongly positive for CD46, CD47, CD56 and CD63 and were also strongly positive for some markers never described before in mesothelioma cell lines, including CD55, CD90 and CD99. (biomedcentral.com)
- By real time PCR we found that our cell lines expressed high mRNA levels of typical mesothelioma markers as mesothelin (MSLN) and calretinin (CALB2), and of BMI-1, a stemness marker, and DKK1, a potent Wingless [WNT] inhibitor. (biomedcentral.com)
- The identification of new mesothelioma markers may be useful for diagnosis and/or prognosis of this neoplasia as well as for isolation of mesothelioma tumor initiating cells. (biomedcentral.com)
CD242
- Furthermore, the cells did not express cytoplasmic mu-heavy chain (cmu) or surface IgM, but were positive for CD24 (heat stable antigen [HSA]) and CD43 (leukosialin), suggesting that the cells produced were blocked at a late pro-B-cell stage. (ox.ac.uk)
- Flow cytometry analysis showed that cells with CD24- CD44+ phenotype were enriched in the culture period (from 2.6% on day 1 to 32.6% on day 22). (celljournal.org)