AnatomyChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesDisciplines and OccupationsInformation ScienceHealth Care
Single-Cell AnalysisFlow CytometryLaser Scanning CytometryMicrofluidic Analytical TechniquesCell SeparationImage CytometryCytological TechniquesCellsRegional Blood FlowFluorescent DyesBlood Flow VelocityFluorescenceLasersStaining and LabelingCell CountEquipment DesignMicroscopy, FluorescenceGreen Fluorescent ProteinsLeukocyte CountImage Processing, Computer-AssistedCells, CulturedClone CellsTime FactorsReproducibility of ResultsAlgorithmsCell DifferentiationPulsatile FlowGene FlowSignal TransductionCell Line, TumorCell LineCerebrovascular CirculationCoronary CirculationRheologyOptic FlowLaser-Doppler FlowmetryFlow Injection AnalysisPeak Expiratory Flow RateVascular ResistanceBlood PressureMicrospheresBlood CirculationModels, CardiovascularHemorheologyMicrocirculationHydrodynamicsUltrasonography, Doppler, ColorXenon RadioisotopesPulmonary CirculationUltrasonography, DopplerCollateral CirculationLiver CirculationStress, MechanicalOxygenPerfusionCardiac OutputRenal CirculationVasodilationOxygen Consumption
PopulationsFluorescenceSorterAssaysCytometricApoptosisFACSCytometerFacilityProliferationSortersImmunophenotyping by flow cytometryDownstreamIntracellularBeckman CoulterBiologyProteinsIsolationLund UniversityCytometersResearchersLasersMolecularReagentsDifferentiationMarkersParticlesAnalyticalAntigenMammalian cellsTumorStem cellVitroDropletsImmunohistochemistryHeterogeneousTissueGenomicsConfocalLaboratoriesProteinEndothelialFluorescentSubsetsBiosafetyMarkerSimultaneouslyGeneCharacteristicsApplicationsClonalPhenotypeMicrofluidicEnablesFlowJoCommonlyImmuneProtocolsExperimentsSingle-cellLaboratoryBSL2Cytometry needs
Populations20
  • Myeloid cell populations isolated from colonic inflammatory bowel disease (IBD) or murine dextran sulphate sodium (DSS) induced colitis were assessed using flow cytometry and compared to healthy controls. (frontiersin.org)
  • We identify two populations of cells with active Wnt signaling: highly proliferative Lgr5 + /Axin2 + cells, as well as secretory Lgr5 − /Axin2 + cells. (nature.com)
  • Lgr5 is a well-established marker of these basal stem cells in the gastrointestinal tract 1 and lineage tracing experiments have demonstrated that Lgr5 + cells give rise to clonal cell populations establishing entire colonic crypts 2 . (nature.com)
  • Fluorescence-activated cell sorting and cDNA-microarray analyses revealed that each subclone was composed of distinct populations of cells. (nature.com)
  • The flow cytometer is equipped with multiple lasers for analysis of multi-fluorescent labelled cell populations and acoustical focusing for improved cell separations. (utsouthwestern.edu)
  • Herein, we show that mTOR complex 1 (mTORC1) and mTORC2 have distinct roles in the generation of CD8 + T cell effector and memory populations. (jci.org)
  • Also available are post-acquisition analysis software packages that provide listmode playback of all experiments in real-time with full analytical adjustment, cell cycle analysis, histogram subtraction and/or overlays of multiple histograms, and color-backgating of discrete cell populations. (auburn.edu)
  • Over the years, cell sorting was combined with molecular biological techniques and proteomics for characterizing metastatic & primary cancer cell populations and single tumor cells. (grandviewresearch.com)
  • Flow cytometry-based sorting plays a significant role in the development of therapeutic products due to its ability to analyze heterogeneous cell populations. (grandviewresearch.com)
  • The lasers are specifically selected for optimal signal-to-noise separation and improve the resolution of dim populations, thereby enabling more effective gating for sorting. (bdbiosciences.com)
  • She has significant knowledge of innate and adaptive immune cell biology and phenotyping, in vitro functional assays, ex vivo analysis of cells from mouse, human and non-human primate (NHP) models, and sorting of multiple immune populations for downstream assays and analyses, which is a significant component of her research methodology. (drexel.edu)
  • Dr. Muir's major interests lie in identifying causes of low recruitment and retention of a diverse healthcare and scientific workforce and highlighting the impact of low representation of diverse patient populations in clinical trials, with some of her work already published in Cell, Transfusion Apheresis Science and Journal of Clinical Anesthesiology . (drexel.edu)
  • Flow cytometry is a powerful technique for the analysis of multiple parameters of individual cells within heterogeneous populations. (cabd.es)
  • Built on the foundations of the BD FACSymphony™ A5 Cell Analyser and the BD FACSAria™ Fusion Cell Sorter, the BD FACSymphony™ S6 System allows the sorting of complex hierarchies of populations identified with 20 or more colours. (bdbiosciences.com)
  • Full spectral cytometry for multi-parametric analysis of cell populations with high autofluorescence and/or low levels of expression of key biomarkers. (embl.org)
  • Complex multi-color analysis of cell populations based on light scatter and fluorescent probes content by conventional and spectral cytometry. (embl.org)
  • Full-service sorting of rare populations out of a heterogeneous sample. (embl.org)
  • The FACS Aria may be used for analysis or for physically separating out cell populations based on their fluorescence labelling. (lu.se)
  • Flow cytometry is widely used for analysis of immune cell characteristics as well as for sorting of pure cell populations. (lu.se)
  • Flow cytometry is one of the major methods in the research of cell populations. (lu.se)
Fluorescence19
  • Methods of cell sorting fall into two major categories: fluorescence activated cell sorting (FACS) and immunomagnetic cell sorting. (wikipedia.org)
  • Due to many years of refinement and increased demand for cell separation however, researchers are working to develop microfluidic sorting devices that have many benefits in comparison to the main types of fluorescence-activated cell sorting and immunomagnetic cell sorting methods. (wikipedia.org)
  • Fluorescence-Activated Cell Sorting, is also known as flow cytometry cell sorting, or commonly known by the acronym FACS, which is a trademark of Becton Dickinson and Company. (wikipedia.org)
  • Fluorescence activated cell sorting utilizes flow cytometry to separate cells based on morphological parameters and the expression of multiple extracellular and intracellular proteins. (wikipedia.org)
  • Fluorescence activated cell sorting has several systems that work together to achieve successful sorting of events of interest. (wikipedia.org)
  • Diagram A shows fluorescence-activated cell sorting of negative cell selection (undesired group) and diagram B shows FACS of positive cell selection (desired group). (wikipedia.org)
  • If you can identify which CD is presented on your cells of interest, then you can stain your sample with a fluorescent dye specific to it and use fluorescence-activated cell sorting to separate the population of interest. (wikipedia.org)
  • The technique helps view cell pictures in 2D by stacking the 1D spatial profiles after demodulating the detected transmission and fluorescence signals. (grandviewresearch.com)
  • On the basis of technology, Fluorescence-based Droplet Cell Sorting (FACS) segment generated the highest revenue in 2018, as it is a highly accurate and precise sorting method. (grandviewresearch.com)
  • These characteristics are determined using an optical-to-electronic coupling system that records how the cell or particle scatters incident laser light and emits fluorescence. (yale.edu)
  • Electrostatic deflection is used to direct the collection of cells, sperm, or other targets according to their fluorescence and/or scatter signatures. (coherent.com)
  • Dr. Muir is also a senior level expert in flow cytometry and fluorescence activated cell sorting (FACS) with 15 years' experience. (drexel.edu)
  • The new BD FACSymphony™ A5 SE Cell Analyzer is a fluorescence-activated, spectral-enabled cell analyzer that offers researchers the ability to choose between spectral or compensation-based cell analysis to meet different flow cytometry needs. (bdbiosciences.com)
  • The new BD FACSymphony™ A1 Cell Analyzer is a fluorescence-activated cell analyzer that offers advanced research capabilities in a compact design, which helps improve access to instrumentation for complex scientific research to more labs. (bdbiosciences.com)
  • Applications also include cell sorting, multiphoton imaging, matrix-assisted laser desorption ionization time-of-flight (Maldi-Tof) for protein analysis, fluorescence analysis in proteomics and genomics, and a novel treatment for ischemic strokes. (photonics.com)
  • In addition, our analytical and sorting flow cytometry capabilities complement the imaging equipment by providing high throughput cell fluorescence measurements. (ca.gov)
  • Positive cells were sorted by fluorescence-activated cell sorting. (bvsalud.org)
  • Flow cytometry enables measurement of fluorescence intensity of particles (cells) in a liquid stream. (lu.se)
  • fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)
Sorter12
  • The incorporation of a flow cytometer and a cell sorter also reduces the cost associated with recurrent use of the main flow cytometry facility on campus. (utsouthwestern.edu)
  • Equipment in this laboratory includes an Attune NxT Flow Cytometer from Life Technologies and a Sony SH800 Cell Sorter. (utsouthwestern.edu)
  • The cell sorter has 4 lasers providing flexibility for experiments with up to 6 colors and with multiple sort collection formats. (utsouthwestern.edu)
  • The fluidic system has to establish a precisely-timedbreak off from the liquid stream in small uniform droplets, so that droplets containing individual cells can then be deflected electrostatically Based on the invention of Richard Sweet, droplet formation of the liquid jet of a cell sorter is stabilized by vibrations of an ultrasonic transducer at the exit of the nozzle orifice. (wikipedia.org)
  • The facility has both analytical and cell sorting instrumentation available including both a Beckman Coulter analyzer and cell sorter. (auburn.edu)
  • The AU Flow Cytometry & High-Speed Cell Sorting Core Laboratory has recently installed a new state-of-the-art compact benchtop cell sorter that is housed within a BSL2-competant laminar flow biosafety cabinet. (auburn.edu)
  • The CytoFLEX SRT Cell Sorter employs ultrasensitive avalanche photodiode detectors (APDs) that can drive 4-way positive cell sorting as well as the ability to capture aborts. (auburn.edu)
  • The sorter also has a robotic arm that can sort into 96 or 384-well plates, with or without index sorting, and employs automated sort setup and monitoring throughout each sort. (auburn.edu)
  • The custom designed Baker SterilGARD Biosafety Cabinet is a Class II Type Biosafety Containment Cabinet designed specifically for the CytoFLEX SRT Cell Sorter. (auburn.edu)
  • The BD FACSAria™ Fusion Flow Cytometer combines sorting capabilities with best-in-class biosafety expertise for a comprehensive advanced cell sorter and biosafety solution. (bdbiosciences.com)
  • A high-speed sorter is available for a wide range of applications including flow karyotyping, chromosome sorting, cell cycle analysis, stem cell isolation, green fluorescent protein (GFP) containing cell sorting, and immunocytochemistry. (childrenshospital.org)
  • Our facility houses one state of the art cell sorter, two analysers and one mutliplex analyser. (lu.se)
Assays8
  • Collectively, both arms of this Module add scientific value to the work of vision scientists by reducing the time and effort required for basic cell culture assays such as viability and cell cycle assessments. (utsouthwestern.edu)
  • and a Nexcelom Cellometer for rapid determination of cell number, cell viability, transfection efficiency, and cell cycle assays. (utsouthwestern.edu)
  • It consists of 4 fluorescent channels (UV to far red) and has extensive applications including bright field cell counting, wound-healing and cell growth assays, cell line development, induced pluripotent stem cell (iPSC) reprogramming, 3D invasion and migration modeling, immune-oncology, virology, and fluorescent-based assays. (utsouthwestern.edu)
  • For successful flow cytometry assays and reproducible results, antibodies validated for use in flow cytometry and proper controls are essential. (sigmaaldrich.com)
  • Our enhanced validation antibodies are characterized using numerous molecular and cell-based techniques and assays to provided superior assurance with target specificity for your required application needs. (sigmaaldrich.com)
  • Extensive support for basic and complex cell cycle analysis and apoptosis assays. (embl.org)
  • Distinct hallmarks of cancer were examined using in silico analysis, various in vitro cell culture assays, and ex vivo organotypic rat brain slice culture model. (hindawi.com)
  • We investigated the impact on tumor malignancy in F98 glioma cells using in silico analysis, in vitro cell culture assays, and ex vivo vascular organotypic glioma impact model (VOGIM) [ 35 ] by implanting F98 cells into rat brain slices. (hindawi.com)
Cytometric8
  • Our mission is to provide comprehensive flow cytometric analysis and sorting including instrumentation, technical support, training and consultation. (yale.edu)
  • Facility provides instrumentation, technical and professional assistance, training and consultations for performing laser-based flow cytometric analysis and sorting. (nationaljewish.org)
  • The LMS/NIHR Flow Cytometry Facility at Imperial aims to facilitate biomedical research by providing a specialist service in flow cytometric analysis and cell sorting. (imperial.ac.uk)
  • The Flow Cytometry Core Facility offers a range of flow cytometric techniques. (embl.org)
  • We offer a wide range of flow cytometric techniques, hands-on service, data analysis, and tailored training sessions for users inside and outside EMBL. (embl.org)
  • Developing of novel flow cytometric techniques to meet EMBL's diverse scientific needs. (embl.org)
  • The Flow Cytometry Core Facility at LUDC provides instrumentation and technical assistance to perform flow cytometric analysis and operator based cell sorting service to all Lund University scientists as well as scientists from other institutes and industry. (lu.se)
  • Flow cytometric immunophenotyping is a highly complex and relatively new technology. (cdc.gov)
Apoptosis6
  • When either the sphere-forming system or the adherent system is used, some of the cancer cells can be propagated but other cells are lost because of cell death/apoptosis or differentiation. (nature.com)
  • Dr. Lim has more than 18 years of experience in cell sorting, cell cycle analysis, apoptosis and immunophenotyping. (sc.edu)
  • Etoposide does not influence phenotype of this sub-population of melanoma cells, while a combined treatment with Etoposide and Bevacizumab significantly abolished P1 sphere-forming ability, an effect associated with apoptosis of this subset of cells. (oncotarget.com)
  • Hypoxic melanoma cells sorted for VEGF-R2/CD133 positivity also undergo apoptosis when exposed to Etoposide and Bevacizumab. (oncotarget.com)
  • J) Annexin V and (K) Ki-67 staining of CD33+ myeloid cells show apoptosis and proliferation, respectively. (eprf.ca)
  • BP act in the regulation of osteoclast function by decreasing bone resorption by different mechanisms, inhibiting the development of their precursor cells, increasing apoptosis rate, stimulating inhibitor factors and reducing its activity 28 . (bvsalud.org)
FACS7
  • Cell sorting using microfluidic technology is growing significantly due to its convenient and fast application as well as lower costs as compared to other techniques such as FACS and MACS. (grandviewresearch.com)
  • The analysis of the samples via FACS Calibur and FACS Canto II can be performed autonomously by the respective user, after the operator's briefing. (unimedizin-mainz.de)
  • Cell sorting with FACS Vantage must be performed by the operator. (unimedizin-mainz.de)
  • The Flow Cytometry Core Facility offers access to high-end FACS analysis and state-of-the-art cell sorting capabilities, actively providing scientists with adaptive setups and dedicated support. (embl.org)
  • Practical parts will be performed on human immune cells in our cell lab, and samples will be analysed on BD FACS Canto and BD FACS Aria II instruments. (lu.se)
  • The Lund Stem Cell Center FACS Core Facility is a state-of-the-art multi-colour flow cytometry facility located at BMC B12 and serves all researchers at Lund University and others. (lu.se)
  • At the facility we currently have four cell sorters (the BD FACS AriaIIu SORP, BD FACS AriaIII, BD FACS Melody and BD FACS Symphony S6) and three analysers (the BD Symphony A1, BD LSR Fortessa SORP and BD LSR Fortessa X20). (lu.se)
Cytometer9
  • Moreover, inclusion of a high throughput imaging cytometer allows for many of these same comparisons to be made without the need for trypsinization and extensive cell processing. (utsouthwestern.edu)
  • In this study, the association between circulating Th17 frequencies and disease activities or laboratory parameters was examined in flow cytometer-sorted CD45RO-positive memory CD4 T cells from SLE. (bmj.com)
  • The Flow Cytometry Laboratory offers flow cytometry analysis on a Beckman Coulter Analytical Flow Cytometer - a CytoFLEX LX flow cytometer - which is used for the qualitative and quantitative measurement of biological and physical properties of cells and other particles. (auburn.edu)
  • The CytoFLEX LX flow cytometer can perform up to 21 color marker analyses simultaneously. (auburn.edu)
  • The software is common across many BD cell analyzers and cell sorters, making the BD FACSAria™ Fusion Flow Cytometer compatible with them. (bdbiosciences.com)
  • Get more information from the BD FACSAria™ Fusion Flow Cytometer brochure . (bdbiosciences.com)
  • The BD FACSAria™ Fusion Flow Cytometer can be purchased with or without a BSC. (bdbiosciences.com)
  • The flow cytometer is used only after previous booking. (unimedizin-mainz.de)
  • A conventional flow cytometer typically uses the same four laser wavelengths: 405 nm, 488 nm, and 640 nm, with an optional 561 nm. (coherent.com)
Facility17
  • The Flow Cytometry Core Facility at the National Eye Institute provides flow cytometry analytical and sorting equipment and services to the NEI Intramural community. (nih.gov)
  • The facility offers comprehensive analytical and sorting services. (nih.gov)
  • Use the Flow Cytometry facility description above for grant proposals. (auburn.edu)
  • The Department is housed in a state-of-the-art facility with core laboratories for flow cytometry, cell biology, and molecular sciences, as well as pathogen containment facilities for small (BSL-3) and large animal research (ABSL-2). (montana.edu)
  • The flow cytometry facility is a shared resource facility providing cell analysis and sorting to the entire UT Southwestern campus. (utsouthwestern.edu)
  • There is a Moflo and three FACSArias available in the facility for sorting. (utsouthwestern.edu)
  • The facility is a focal point for research involving microscopy and cell imaging. (nationaljewish.org)
  • The central mission of our Berkeley CIRM Shared Stem Cell Facility (SSCF) is to provide our East Bay users with knowledge, expertise, training, and equipment to advance scientific knowledge of human embryonic and other stem cells. (ca.gov)
  • Our facility is designed to support human embryonic stem cell (hESC) culture, including high quality cell culture space equipped with biosafety cabinets, incubators, cryogenic storage, and a standard microscope. (ca.gov)
  • Finally, the facility is equipped with a laminar flow hood to conduct chemistry to create biomaterials and micropatterned surfaces for stem cell culture, as well as subsequent analysis by imaging and flow. (ca.gov)
  • We are very thankful to CIRM for enabling the construction and development of this state-of-the-art stem cell facility. (ca.gov)
  • Quantification of gene delivery efficiency and gene targeting has been greatly aided with the flow cytometry and high throughput imaging capabilities of the CIRM facility. (ca.gov)
  • He is establishing laser ablation using the multiphoton microscope in the CIRM facility, a capability that will be applied to study cytoskeletal mechanics of stem cells. (ca.gov)
  • Our facility strives to meet researchers' specific needs by providing dedicated help with experimental design, enabling the highest possible resolution in terms of analysis and product. (embl.org)
  • We have recently upgraded the facility to provide access to S2 cell sorting services and improved our general sorting set up to meet the growing need for high-parametric and aseptic sorting. (embl.org)
  • Housed in the IDDRC Stem Cell Core Facility, this core offers cost effective, state-of-the-art flow cytometry and cell sorting services. (childrenshospital.org)
  • The Flow Cytometry Shared Resource facility is able to comply with these standards only at the level of BSL2 with enhanced precautions. (cuanschutz.edu)
Proliferation9
  • Ly6C high MC presented downregulated co-stimulatory receptors (CD2, GITR, and TIM1) which direct immune cell proliferation, and upregulated co-stimulatory ligands (LIGHT and SEMA4A) which trigger antigen priming and differentiation. (frontiersin.org)
  • Endothelial cell lineage tracing showed that BNP directly stimulated the proliferation of resident endothelial cells via NPR-A binding and p38 MAP kinase activation. (elifesciences.org)
  • BNP also stimulated the proliferation of WT1 + epicardium-derived cells but only in the hypoxic area of infarcted hearts. (elifesciences.org)
  • The origin of new endothelial cells (i.e. resident or infiltrating) as well as the underlying mechanism leading to their proliferation (partial endothelial-to-mesenchymal transition [EndMT] or not) have long been debated. (elifesciences.org)
  • In addition to providing biomechanical support to the islets, ECM components are required for β-cell development, proliferation, survival, and proper insulin secretion. (nature.com)
  • FLT3 ITD triggers the proliferation of the quiescent hematopoietic stem cell (HSC) pool but fails to directly transform HSCs. (biomedcentral.com)
  • Our results further indicate that MCT4 promotes proliferation and survival by altered cell cycle regulation and cell death mechanisms. (hindawi.com)
  • For this purpose, we designed an MCT4 overexpression and knockdown/inhibition model to explore the functional consequences of MCT4 expression on cancer cell metabolism, survival, proliferation, migration, invasion potential, and angiogenesis as well as on cell cycle profiles and cell death mechanisms. (hindawi.com)
  • This is a basic course in flow cytometry, which covers various applications of the technique, such as analysis of extracellular and intracellular proteins, analysis of cell viability and cell proliferation, detection of proteins in solution and cell sorting. (lu.se)
Sorters4
  • Moreover, flow cytometry cell sorters are complex instruments that are generally used only by well-trained staff in flow cytometry facilities or well-equipped laboratories and, since they are normally big in size, it is not always possible to place them inside a biological safety cabinet. (wikipedia.org)
  • Growing research in cell sorting technologies, technological advancements in cell sorters, and rising pharmaceutical & biotechnology companies are expected to drive growth. (grandviewresearch.com)
  • The market is segmented into cell sorters, services, and reagents & consumables. (grandviewresearch.com)
  • Cell sorters dominated the market in 2018, owing to their advanced technology. (grandviewresearch.com)
Immunophenotyping by flow cytometry2
  • The process of measuring the percentage of CD4+ T-lymphocytes in the whole blood sample is referred to as ``immunophenotyping by flow cytometry'' (9-14). (cdc.gov)
  • This document provides CDC recommendations for immunophenotyping by flow cytometry. (cdc.gov)
Downstream4
  • The aim is to give you practical recommendations on how to succeed with Cell Sorting for downstream single-cell analyses. (ki.se)
  • The Genesis system automates cDNA synthesis, pairing each cell with a unique cellular barcode and molecular indice for downstream analysis. (genomeweb.com)
  • Celsee claims that the Genesis tool can sort cells in about 20 minutes and capture at least 70 percent of analyzed cells for downstream analysis. (genomeweb.com)
  • The possibility to do single cell sorting makes it a strong tool for single cell analysis in downstream applications. (lu.se)
Intracellular7
  • Cell sorting is the process through which a particular cell type is separated from others contained in a sample on the basis of its physical or biological properties, such as size, morphological parameters, viability and both extracellular and intracellular protein expression. (wikipedia.org)
  • We examined frequencies of Th17 cells by sorting the purified CD4 T cells bearing the CD45RO marker, followed by intracellular IL-17A staining after in vitro activation. (bmj.com)
  • Frequencies of Th1 and T Foxp3 cells were also measured by intracellular IFN-γ and Foxp3 staining, respectively. (bmj.com)
  • Using PBMC from 48 patients with SLE and 48 age- and sex-matched healthy individuals, we examined the frequency of Th17 cells in purified CD4-positive T cells bearing CD45RO, a well-known differentiated memory T cell marker, followed by intracellular IL-17A staining upon in vitro activation. (bmj.com)
  • CT serves as a model for AB type toxins that act by ADP-ribosylation of an intracellular G-protein in the target cell. (mdpi.com)
  • The fusogenic lectin-like property of CT has been explored for membrane transport, cell signalling, host cell biology and controlling intracellular machineries. (mdpi.com)
  • Analysis of live and fixed cells may be based on size, granularity/complexity, fluorescent proteins, cell surface antigen expression, intracellular antigen expression, DNA content. (lu.se)
Beckman Coulter1
  • We work with equipment from Beckman Coulter, Becton Dickinson, Cytek Biosciences, and Coherent Inc. The EMBL Flow Cytometry platform is open to test new technological developments to serve the needs of the scientific community. (embl.org)
Biology9
  • She serves on NIH study sections and as reviewer for journals in her area of expertise, manages an active laboratory, supervises students in pulmonary biology and epithelial cell research and has received awards recognizing both teaching and original research accomplishments. (rochester.edu)
  • Flow cytometry-based sorting has been widely used by researchers in biology laboratories for cell isolation. (grandviewresearch.com)
  • Flow cytometry is a powerful molecular and cellular biology tool that enables multi-parametric analysis of individual cells. (sigmaaldrich.com)
  • It will enable stem cell biology and mammalian cell-based protein manufacturing, which the company said are both fast-developing fields. (genomeweb.com)
  • The Ocular Cell Biology research program at QUT (Kelvin Grove Campus, Brisbane) is seeking to appoint a biomedical scientist to lead the design and implementation of protocols for the immunophenotyping and sorting of cells isolated from human corneas. (timeshighereducation.com)
  • The Ocular Cell Biology research program at QUT (Kelvin Grove Campus, Brisbane) resides within the School of Biomedical Sciences and is one of several leading programs within the Centre for Vision and Eye Research. (timeshighereducation.com)
  • For over twenty years, the Ocular Cell Biology research program, led by Professor Damien Harkin, has been assisting clinicians in their efforts to develop improved methods for the treatment of patients with eye disease. (timeshighereducation.com)
  • Experience in dendritic cell biology and antigen presentation mechanisms is a merit, T cell biology is also attractive. (lu.se)
  • Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)
Proteins3
  • Rspo proteins are secreted and can stabilize the effects of Wnt ligands by preventing ubiquitination and turnover of the Wnt receptor frizzled 9 , thereby dictating the size of the Lgr5 + stem cell pool by regulating self-renewal of Lgr5 + cells 10 . (nature.com)
  • These are engineered using antibody biochemistry so that they stick only to specific antigens (usually proteins) on the outside of the cells. (coherent.com)
  • Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)
Isolation3
  • Loss of the ECM during the islet isolation process is considered to be a significant factor in the poor engraftment rate of transplanted β-cells, thus limiting cell replacement therapy to diabetes 6 . (nature.com)
  • Tools like flow cytometry and lasers offer expedited cell isolation and differentiation. (genomeweb.com)
  • Prior experience in techniques for the cultivation and/or isolation of mammalian cells from blood or tissue. (timeshighereducation.com)
Lund University1
  • Below you can see some examples of the infrastructure for research on genes and cells, available for researchers at Lund University. (lu.se)
Cytometers3
  • The flow cytometers performs this analysis by passing thousands of cells per second through a laser beam and capturing the light that emerges from each cell as it passes through. (cabd.es)
  • Flow cytometers have the ability to measure multiple celular parameters and markers of a large number of cells at a rapid rate. (cabd.es)
  • OptoRobotix develops flow cytometers with cell sorting capabilities. (genomeweb.com)
Researchers11
  • The goal of the Cell Culture and Cell Phenotyping Module is to streamline cell culture and phenotyping analyses for vision researchers. (utsouthwestern.edu)
  • Researchers can use a variety of fluorescent dyes to design multi-color panels to achieve successful, simultaneous sorting of multiple, precisely defined cell-types. (wikipedia.org)
  • In the journal Cell, in August 2018, researchers announced the development of Image-Activated Cell Sorting (IACS). (grandviewresearch.com)
  • According to researchers, a novel product based on microfluidic technique facilitates separation of circulating tumor cells from the peripheral blood of patients suffering from cancer. (grandviewresearch.com)
  • The Flow Cytometry Unit provides scientific and technological support to CNB research groups,as well as to researchers from the public and private sectors. (csic.es)
  • Provides support to NJH researchers in mass spectrometric analyses of lipids and other small molecules in virtually all biological matrixes. (nationaljewish.org)
  • Researchers can use a variety of methods to prepare single cells for analysis. (genomeweb.com)
  • After preparing a liquid, tissue, or cell suspension sample, researchers use the platform's Celsingle micro-analysis slides - which Celsee said can have the capacity for up to 1 million cell microwells - to isolate individual cells. (genomeweb.com)
  • Coe noted that Genesis will allow researchers to process up to 4 ml of fluid at a time using the "appropriate enrichment or preparation slide" and routinely analyze 100,000 single cells in one sample run. (genomeweb.com)
  • Flow cytomery applications used by EMBL researchers: Cell cycle analysis and Phenotyping of immune cells. (embl.org)
  • We offer to our researchers' advice and dedicated training sessions in the use of flow cytometry, instrument operation, and post-acquisition data analysis. (embl.org)
Lasers2
  • The system has the maximum of 6 lasers installed (375 nm, 405 nm, 488 nm, 561nm, 638 nm, 808 nm) and can analyze cells in 21 unique fluorescent color channels. (auburn.edu)
  • Flow cytometry uses lasers to count or sort different types of cells and other biological particles. (coherent.com)
Molecular3
  • Specific areas of interest include understanding the mechanisms, which allow cancer cells to develop resistance to chemotherapy at the cellular and molecular levels. (sc.edu)
  • Finally, the Kumar lab studies how the molecular and mechanical properties of the cellular cytoskeleton regulate cell function. (ca.gov)
  • C.P. tion stage, why much more detailed molecular analysis is contributed to the design of the study, discussions on statistical analysis of called for [2]. (lu.se)
Reagents1
  • With increasing applications of sorting technologies, cell labeling reagents have become widely available in the market. (grandviewresearch.com)
Differentiation5
  • Activation of mTOR-dependent pathways regulates the specification and differentiation of CD4 + T effector cell subsets. (jci.org)
  • One complementary therapy could be 're-activating' vasculogenesis (i.e. the differentiation of precursor cells into mature endothelial cells), a mechanism that occurs in the heart during development but is quiescent in adult hearts. (elifesciences.org)
  • A switch from glycolysis to mitochondrial respiration drives these cells toward differentiation, but the mechanisms that control this switch are poorly defined. (lww.com)
  • Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repres- sion and transcriptional activation. (lu.se)
  • In this study, highly purified, flow-cytometry sorted, classified in relation to normal B-cell differentiation [1]. (lu.se)
Markers9
  • It has recently been shown that murine intestinal macrophages are continually repopulated from circulating blood monocytes, identified by expression of the cell surface markers Ly6C, CCR2, and CD62L ( 6 ). (frontiersin.org)
  • Flow cytometry cell sorting yields very high specificity according to one or several surface markers, but one limitation is constituted by the number of cells that can be processed during a work-day. (wikipedia.org)
  • Interestingly, higher frequencies of IL-17-producing CCR6/CD45RO-positive memory T cells are found in patients with SLE carrying the type I interferon signature, but there are few published reports characterising the memory cell markers on CD4-positive IL-17-producing T cells. (bmj.com)
  • Antibodies are used in flow cytometry to help characterize markers on the cell surface since they allow for the detection of specific antigens. (sigmaaldrich.com)
  • Flow cytometry (CMF) is a technology of multiparametric cell analysis for detection and identification of molecules and cell structures using fluorescent markers and conjugated antibodies. (csic.es)
  • Multicolor flow cytometry is a powerful technology that enables the simultaneous analysis of multiple markers at the single-cell level. (bdbiosciences.com)
  • assessed by immunohistochemistry or flow cytometry, by using a handful of phenotypic markers. (lu.se)
  • CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
  • 2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)
Particles3
  • Flow cytometry is a technique for counting and examining microscopic particles, such as cells and beads, by suspending them in a stream of fluid and passing them by a detection apparatus. (nih.gov)
  • Flow cytometry analysis is a technique commonly used to examine physical and chemical characteristics of cells and particles. (sigmaaldrich.com)
  • Flow cytometry is a technology that simultaneously measures and then analyzes multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. (yale.edu)
Analytical1
  • Analysis of the data is performed on CytExpert Analytical Software. (auburn.edu)
Antigen4
  • CD4 T helper (Th) cells can differentiate from naïve status into antigen-experienced effectors with distinct characteristics, such as the interleukin 17 (IL-17)-secreting Th17 subset. (bmj.com)
  • Neither the B-lymphocyte antigen CD19 nor the T-cell surface glycoprotein CD3 gamma chain (hybridization with a dual probe showed an absence of the fusion (Physique S3A). (eprf.ca)
  • Rosa et al, Science Immunology 2022 ) and has shown recently that the same combination of factors can reprogram tumor cells into antigen presenting cells (Zimmermannova et al, In revision). (lu.se)
  • The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
Mammalian cells2
  • The OPTISORT platform will be a sorting and analysis tool that uses optical and droplet technologies for sorting fragile mammalian cells. (genomeweb.com)
  • A minimum of 3-years of experience in designing and implementing protocols for the immunophenotyping and sorting of mammalian cells. (timeshighereducation.com)
Tumor13
  • Although this heterogeneity is generally discussed in terms of pathological structures, examining the dynamic heterogeneity at the cellular level is fundamental to understanding the origins of the cells, potential therapeutic targets and the source of tumor recurrences 3 . (nature.com)
  • Therefore, functionally analyzing the individual types of the heterogeneous cells and determining their role in tumor pathogenesis are critical. (nature.com)
  • The cancer stem cell (CSC) hypothesis, which was first described in studies on leukemia, has attracted considerable attention in other cancer fields, including the one devoted to brain tumor 4 . (nature.com)
  • They constitute a pool of self-renewing cells with the ability of sustaining tumor growth and promoting metastatic dissemination. (oncotarget.com)
  • However, most GEP studies have typically been performed on whole tissue samples, containing varying degrees of tumor cell content, which results in uncertainties in data analysis. (lu.se)
  • GEP analyses were performed on highly purified, flow-cytometry sorted tumor-cells from eight subgroups of BCLs. (lu.se)
  • This enabled identification of TFs that can be uniquely associated to the tumor cells of chronic lymphocytic leukemia (CLL), diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL), hairy cell leukemia (HCL), and mantle cell lymphoma (MCL). (lu.se)
  • Formerly known as DeNovo Sciences, Celsee changed its name in 2016 to Celsee Diagnostics to reflect its focus on developing commercial tools based on its circulating tumor cell enrichment products. (genomeweb.com)
  • In summary, our data highlight MCT4 /SLC16A3 as a key gene for distinct hallmarks of tumor malignancy in glioma cells. (hindawi.com)
  • This process enables tumor cells, including those within the core of the heterogeneous tumor mass to survive, despite being at a distance from blood vessels that supply their metabolic needs [ 16 , 17 ]. (hindawi.com)
  • In the metabolic symbiosis model, these cells have been proposed to overexpress MCT4 to release high amounts of lactate produced during anaerobic glycolysis into the tumor microenvironment. (hindawi.com)
  • We here aimed to investigate the anti-tumor activity of a novel monoclonal antibody (mAb) 11C9 and identify the antibody tractable target in the hepatocellular cancer stem cells (HCSCs). (bvsalud.org)
  • This project will aim to test a cancer immunotherapy approach based on in-vivo dendritic cell reprogramming of tumor cells to reinstate anti-tumor immunity. (lu.se)
Stem cell7
  • Signals that regulate epithelial regeneration upon stem cell injury are largely unknown. (nature.com)
  • Expression of Lgr5 is controlled by Wnt signaling, which plays a critical role for stem cell turnover in the gastrointestinal tract 2 . (nature.com)
  • We have shown that in the stomach, stem cell homeostasis is regulated by Wnt and Rspo secreted by stromal myofibroblasts 11 . (nature.com)
  • Hence, recapitulating the islet ECM in both isolated islets and stem-cell-derived β-cells is of high therapeutic value. (nature.com)
  • NEW YORK (GenomeWeb News) - UK-based microfluidics developer Dolomite said today that it will collaborate with the Danish firms Bioneer and OptoRobotix in a European Union-funded effort to develop a new stem cell handling device. (genomeweb.com)
  • Seeks to conduct and enable research and NJH aimed at stem cell characterization, regeneration of the lung, and the genetic editing of stem and other primary cells. (nationaljewish.org)
  • As an additional example, the Li lab has been utilizing micropatterned surfaces of variable mechanical properties to study the effects of substrate mechanics on stem cell function, and these efforts have been enabled by the high throughput imager. (ca.gov)
Vitro6
  • Our results suggest that single cell-derived subclones from a patient can produce phenotypically heterogeneous self-renewing progenies in both in vitro and in vivo settings. (nature.com)
  • Regardless of the nomenclatures used, the CSCs (as the cells are commonly termed) that are isolated directly from patients' tumors are considered to serve as valuable tools that can enhance our understanding of tumorigenesis, therapeutic resistances and the functional heterogeneity of cancers in vitro and in vivo 5 . (nature.com)
  • 1 , 2 Patients with systemic lupus erythematosus (SLE) have increased levels of IL-17 in blood and renal tissues, and higher frequencies of Th17 cells are found by directly measuring CD4-positive IL-17-producing T cells upon in vitro activation of peripheral blood mononuclear cells (PBMC), with inconsistent results regarding the correlation between IL-17 levels or frequencies of Th17 cells and disease activities. (bmj.com)
  • 3 , 4 Indeed, there are difficulties in defining human CD4-positive IL-17-producing cells by flow cytometry because of the very low frequencies in PBMC samples and the problem of background after in vitro activation. (bmj.com)
  • As one example, the Robey and Winoto labs are developing and optimizing in vitro culture systems to induce T cell development from human embryonic stem cells and iPS cell lines. (ca.gov)
  • Single-cell sorting for single cell quantitative genome, transcriptome, CRISPR/Cas9 genome editing and in vitro studies. (embl.org)
Droplets3
  • This method allows multiparameter cell sorting and involves encapsulating cells into small liquid droplets which are selectively given electric charges and sorted by an external electric field. (wikipedia.org)
  • During the separation of the droplet with the cell in it from the intact liquid jet, a voltage pulse is given to the liquid jet so that droplets containing the cells of interest can be deflected in an electric field between two deflection plates for sorting. (wikipedia.org)
  • In a sorting instrument, the flow passes through a nozzle prior to the interaction zone that ensures the cells are individually held in tiny droplets which pick up a small static charge from the nozzle. (coherent.com)
Immunohistochemistry2
  • Antibodies in 25 μg or 25 μL small pack sizes for your immunocytochemistry (ICC), immunohistochemistry (IHC), immunofluorescence (IF), immunoprecipitation (IP), flow cytometry, ELISA and western blot applications. (sigmaaldrich.com)
  • Practical experience with multiparametric flow cytometry, cell sorting and immunohistochemistry /immunofluorescence microscopy, or immunopeptidomics is a merit. (lu.se)
Heterogeneous2
  • Cancers are composed of heterogeneous combinations of cells that exhibit distinct phenotypic characteristics and proliferative potentials. (nature.com)
  • GBM, as its name suggests, is composed of a pathologically heterogeneous mixture of cells that exhibit varying degrees of cellular and nuclear polymorphism 2 . (nature.com)
Tissue8
  • Monocytes (MC) are bone marrow (BM) derived mononuclear phagocytes that play an important role in innate immune response and are the major immune cell population in chronic tissue inflammatory ( 1 , 2 ). (frontiersin.org)
  • Results from murine studies ( 2 ) combined with the observation of radiolabelled blood monocytes in the inflamed intestinal mucosa of IBD patients ( 15 ), indicate that increased LP inflammatory mononuclear cells are a result of monocyte recruitment, rather than expansion of a tissue resident macrophage population. (frontiersin.org)
  • IMs are found in the space between the tissue stroma cells and contain, among other components, fibrillar collagens and fibronectin. (nature.com)
  • Cells from solid tissue must be disaggregated before analysis. (yale.edu)
  • The NJH lab provides a resource for storage of multiple cell tissue samples, technical consultation, assay development, training for CTRC investigators, staff, visiting physicians, and students. (nationaljewish.org)
  • Photobleaching and low penetration depth, caused by the high scattering of the visible light, limit the utility of confocal microscopy in certain applications, particularly with living cells and/or thicker tissue samples. (photonics.com)
  • High-throughput sorting of tissue-specific nuclei from Drosophila melanogaster embryo as a preparative step in the analysis of genome regulatory activity during tissue development. (embl.org)
  • The protein protects tissue from inflammation when cells rupture. (lu.se)
Genomics4
  • Technology breakthrough has potential to transform wide range of disciplines from immunology and genomics research to cell-based therapeutics. (bdbiosciences.com)
  • NEW YORK (GenomeWeb) - With the commercial launch of its single-cell analysis platform today, Celsee aims to tap into the expanding markets for proteogenomics, immune monitoring, and single-cell genomics. (genomeweb.com)
  • Some early users from a large non-profit genomics research institute who wished to remain anonymous due to the status of their early-stage research explained that they are using Genesis to profile single cells related to their research. (genomeweb.com)
  • This core provides projects with assistance in designing integrative genomics studies and in data analysis and interpretation. (childrenshospital.org)
Confocal2
  • Instrumentation suites house equipment for DNA sequencing, genomic analysis, flow cytometry and cell sorting, and confocal microscopy. (montana.edu)
  • Because the amount of laser energy that is actually absorbed is relatively low, multiphoton imaging is much more compatible with live-cell studies than is scanning confocal laser microscopy. (photonics.com)
Laboratories3
  • The Cell Culture and Cell Phenotyping (CCCP) laboratories are located within the Department of Ophthalmology on the 7th floor of the Florence (E) Building. (utsouthwestern.edu)
  • In blood cell analysis applications in both research and clinical laboratories, the cells are first treated with a number of fluorescent labels (fluorochromes). (coherent.com)
  • Regulatory standards require that cell sorting laboratories comply with BSL2 requirements at a minimum. (cuanschutz.edu)
Protein5
  • This can be attributed to the growing cellular analysis in the field of cancer, stem cells, drug discovery, protein engineering , and cell engineering. (grandviewresearch.com)
  • To study the potential role in this metabolic shift of von Hippel-Lindau (VHL), a protein component of a ubiquitin ligase complex, the authors generated nephron progenitor cell-specific VHL knockout mice. (lww.com)
  • G and H) Representative immunoblots show increased pSTAT5 and total STAT5 (G), and pERK (H) protein expression levels in NS/JMML CD33+ myeloid cells. (eprf.ca)
  • Establishment of clonal cell lines carrying fluorescent protein-tagged genome-edited genes for 4D life cell imaging, and assessment of protein interactions and concentrations during mitosis. (embl.org)
  • In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)
Endothelial5
  • BNP treatment increased vascularisation and the number of endothelial cells in all areas of infarcted hearts. (elifesciences.org)
  • Our results demonstrated that these immature cells have a natural capacity to differentiate into endothelial cells in infarcted hearts. (elifesciences.org)
  • While some islet BM components are produced by endothelial cells (ECs), the source of others remains unknown. (nature.com)
  • A multiphoton excited image such as this bovine pulmonary endothelial cell relies on an ultrafast laser. (photonics.com)
  • Other proposed mechanisms include passage of cell-free virus into the brain and release of the virus from the infected endothelial cells. (medscape.com)
Fluorescent2
  • The biosafety hood is certified for BSL2 level sorting and will sort at up to 40,000 events per sec processing fluorescent events using a fully digital system with 7 decades data display using CytExpert software. (auburn.edu)
  • Multiline output is critical because it allows selection of the wavelength that offers the highest excitation cross section for the multiple fluorescent labels that are employed in cell imaging. (photonics.com)
Subsets4
  • Human MC were initially divided into three subsets based on the cell surface expression of CD14 and CD16, and recently classified based on CD40 expression ( 2 - 5 ). (frontiersin.org)
  • The SLE disease activity index (SLEDAI) and other laboratory parameters were further correlated with frequencies of different T cell subsets. (bmj.com)
  • She has authored many publications in her field, with a focus on how specialized cell subsets in the follicles can be targeted for novel vaccines against HIV and other viral infections. (drexel.edu)
  • Dr. Muir's recent work examines how cell subsets of the GC such as T follicular helper cells and GC B cells are known to be dysregulated in acute and chronic infections. (drexel.edu)
Biosafety1
  • The biosafety cabinet relies on the flow of air to provide protection of personnel and containment by an intake air velocity of 100 feet per min (fpm) or more through the front access. (auburn.edu)
Marker1
  • Flow cytometry analysis showed that this differentiating lines expressed the pan-hematopoietic marker CD45 at least until Day 28 (Physique S2B). (eprf.ca)
Simultaneously1
  • By adding specific fluorophores to specific antibodies, cells can be sorted and analyzed simultaneously. (sigmaaldrich.com)
Gene5
  • Welcome to a seminar in the KI Flow Cytometry Seminar Series on the topic of sorting for single-cell analyses, on Wednesday 16 March, at 10:30, in room Gene, NEO. (ki.se)
  • RNA-seq was performed in blood Ly6C high and Ly6C low MC sorted by flow cytometry from control and HHcy cystathionine β-synthase gene-deficient ( Cbs -/- ) mice. (frontiersin.org)
  • Whereas α2 laminins promoted islet cell clustering, they did not affect gene expression. (nature.com)
  • As another example, the Schaffer lab has engineered new gene delivery vehicles that can augment and edit the genome of hESCs and neural stem cells. (ca.gov)
  • Demonstrated ability to perform basic analyses of gene expression (e.g. (timeshighereducation.com)
Characteristics1
  • Several studies have demonstrated that tumors contain a small subset of cells that share many characteristics with CSCs [ 4 ]. (oncotarget.com)
Applications9
  • The homogeneous cell population obtained after sorting can be used for a variety of applications including research, diagnosis, and therapy. (wikipedia.org)
  • These last considerations are of particular importance when cell sorting is used for clinical applications, for example cell therapy and should therefore be performed under Good Manufacturing Practice (GMP) conditions. (wikipedia.org)
  • It also improves collection efficiency and optimizes resolution needed for multicolor applications, even at high-speed sorting settings. (bdbiosciences.com)
  • The flow cell is designed to improve resolution for side population applications and DNA cell cycle analyses. (bdbiosciences.com)
  • For some research applications such as oncology, immunology, and drug discovery, flow cytometry can also be used to sort cells, i.e., to selectively collect a target type of cell while discarding all the others. (coherent.com)
  • So the applications of flow cytometry are numerous, and this has led to the widespread use of these instruments in biological research. (cabd.es)
  • Provides primary human cells to faculty to support research projects and NIH grant applications. (nationaljewish.org)
  • The firm later changed its name again to Celsee as it turned its attention primarily to research applications for its single-cell analysis tech. (genomeweb.com)
  • Preparation of spatial data, developing applications for mobile devices, GIS analysis and modeling, etc. (lu.se)
Clonal1
  • Because most cancers have a clonal origin, cancer stem cells (CSCs) must generate phenotypically diverse progenies including mature CSCs that can self-renew indefinitely and differentiated cancer cells that possess limited proliferative potential. (nature.com)
Phenotype2
  • however, due to constitutive mTORC1 activation, these cells retained a terminally differentiated effector phenotype and were incapable of transitioning into a memory state. (jci.org)
  • CTRC Core Laboratory Services support IRB-approved protocols for clinical research with a wide range of protocols requiring analysis of the function and phenotype of immunologic cells. (nationaljewish.org)
Microfluidic2
  • This instrument integrates microfluidic, optical, computational, electrical, and mechanical technologies for sorting cells on the basis of their global phenotypic profiles and morphological & spatial properties, using an image-driven approach. (grandviewresearch.com)
  • Due to the non-magnetic cell sorting technology, instruments with microfluidic technology speed up the process, sorting billions of cells per second. (grandviewresearch.com)
Enables2
  • It enables 99% cell purity and cell output of 48,000/s. (grandviewresearch.com)
  • It has major advantages such as the simultaneous detection of a wide range of parameters that enables the understanding of diversity among seemingly similar cells. (lu.se)
FlowJo2
  • We also have FlowJo and GemStone Software available for analysis. (nih.gov)
  • Familiarity with the operation of FlowJo and/or FACSDiva software packages for analysis and presentation of flow cytometry data. (timeshighereducation.com)
Commonly1
Immune5
  • However, it is not clear how gut macrophages, relative to recruited blood monocytes and other myeloid cells, contribute to the intestinal inflammatory milieu, nor how macrophages and their monocyte precursors mediate recruitment of other immune cells to the inflamed intestine. (frontiersin.org)
  • The gastrointestinal (GI) tract is exposed to the greatest antigenic burden in the body, which poses a significant challenge to the many resident immune cells ( 1 ). (frontiersin.org)
  • The peri-islet membrane, which encapsulates the islets, separates the endocrine cells from the exocrine pancreas and serves as a barrier from immune cell infiltration to the islets. (nature.com)
  • Dr. Muir's research also looks at the role of multiple adjuvant candidates that aim to improve vaccine responses by enhancing the innate immune and Tfh-B cell program, such as molecules that activate the STING pathway and the enzyme adenosine deaminase-1 (ADA-1). (drexel.edu)
  • In addition to proteogenomics, Celsee said it plans to market the platform for immune monitoring as an alternative to flow cytometry, as well as for the expansion and scale of next-generation sequencing library preparation. (genomeweb.com)
Protocols1
  • The term multi-parameter flow cytometry refers to counting analysis instruments and protocols capable of assaying tens of different parameters. (coherent.com)
Experiments2
  • The Cell Phenotyping arm of this module is designed to provide technical expertise and assistance with ongoing cell phenotyping experiments. (utsouthwestern.edu)
  • The ability to enumerate very large number of cells in a relatively short period of time provides experiments with reliability. (lu.se)
Single-cell3
Laboratory2
  • In laboratory settings, glioma stem cells (GSCs) can be isolated by adding EGF and FGF to suspension cultures in the absence of serum, which is the so-called sphere-forming method, or by using adherent culture systems in which specific materials are coated on culture dishes 4 , 6 . (nature.com)
  • The Flow Cytometry Laboratory at Auburn University provides operator-managed and assisted support for flow cytometry analyses and high-speed cell sorting. (auburn.edu)
BSL21
  • As cell sorting is known to create aerosols, many organisms which can normally be safely handled at the BSL2 level require BSL3 precautions due to the creation of aerosols. (cuanschutz.edu)
Cytometry needs1