• TYRO3 or AXL gene knockdown reduced cell proliferation/colony formation. (nature.com)
  • By utilising flow cytometry, researchers can accurately identify and measure specific cell populations within complex mixtures, as well as assess cell cycle progression, proliferation status, and expression of fluorescent markers. (embl.org)
  • Here, we determined the effect of Jiyuan oridonin A (JOA), an ent -kaurene diterpenoid compound, on the differentiation blockade in imatinib-sensitive, particularly, imatinib-resistant CML cells with BCR-ABL-T315I mutation by cell proliferation assay, apoptosis analysis, cell differentiation analysis, cell cycle analysis and colony formation assay. (jcancer.org)
  • We found that JOA at lower concentration significantly inhibited the proliferation of CML cells expressing mutant BCR-ABL (T315I mutation included) and wild-type BCR-ABL, which was due to that JOA induced the cell differentiation and the cell cycle arrest at G0/G1 phase. (jcancer.org)
  • BCR-ABL oncogene encodes a BCR-ABL tyrosine kinase (TK), which triggers different downstream targets including c-Myc, STAT5 and CrkL, which participated in the control of cell differentiation, proliferation, migration and other cellular behaviors [ 3 - 5 ]. (jcancer.org)
  • Explore several methods to analyze cell cycle, cell proliferation, apoptosis, phosphorylation and cytokine secretion. (bdbiosciences.com)
  • Cell cycle analysis showed that visfatin increased S-stage percentage and proliferation index in a dose- and time-dependent manner. (thieme-connect.com)
  • Potential synergistic effects of various combinations including these compounds were tested by measuring cancer cell proliferation and apoptosis. (frontiersin.org)
  • Poisson-event-based analysis of cell proliferation. (swansea.ac.uk)
  • Taken together, our results suggested that decreased LAPTM5 inhibited proliferation and viability, as well as induced G0/G1 cell cycle arrest possibly via deactivation of ERK1/2 and p38 in BCa cells. (spandidos-publications.com)
  • Furthermore, previous studies suggested that knockdown of LAPTM4B , another important subtype of the LAPTM family inhibited proliferation of hepatocellular carcinoma ( 11 ), prostate ( 12 ) and breast cancer cells ( 13 ). (spandidos-publications.com)
  • Extracts prepared from Trametes versicolor under the trade name of I'm-Yunity exhibit anti-tumorigenic activities, as supported by inhibition of the proliferation and induction of apoptosis in malignant cells. (spandidos-publications.com)
  • The aim of this study is to investigate potential synergistic effects of Flavopiridol and Lonfarnib inhibiting the proliferation of human ovarian cancer cells in vitro. (uni-marburg.de)
  • End points are proliferation, cell cycle allocation, induction of apoptosis and necrosis. (uni-marburg.de)
  • The following methods were applied: at first proliferation assays with photometric analysis were conducted, then an annexin-V-detection was applied for differentiation of the treated cells in vital, necrotic and apoptotic groups. (uni-marburg.de)
  • Analysing the results of the proliferation assays synergistic effects when combining Flavorpiridol and Lonafarnib could not be shown in SKOV3-cells but in BG1-cells. (uni-marburg.de)
  • Down-regulation of EIF5B significantly decreased proliferation and invasiveness of the HCC cells. (techscience.com)
  • Its roles include regulation of proliferation, promotion of immune system and activation of apoptosis in B cells. (intechopen.com)
  • In these cells, IL-21 does not promote proliferation but enhances apoptosis and chemotaxis. (intechopen.com)
  • Further, the results of miR-4755-5p mimic transfection confirmed that under the action of NaF, miR-4755-5p overexpression reduced Cyclin D1 protein expression within osteoblasts and further inhibited cell proliferation and activation. (fluoridealert.org)
  • Then, the relatively insensitive gastric cancer cell lines (MGC-803 and BGC-823) were treated with low concentrations of chidamide alone, bortezomib alone, or chidamide and bortezomib combination to detect the effects on cell proliferation, apoptosis, migration, and invasion. (dovepress.com)
  • Compared with low-dose chidamide or bortezomib alone, the low-dose drug combination significantly inhibited the proliferation, migration, and invasion of MGC-803 and BGC-823 cells and induced apoptosis of the cells. (dovepress.com)
  • Our results suggest that the combination of chidamide and bortezomib can significantly reduce the proliferation, invasion, and migration of MGC-803 and BGC-823 cells, providing a framework for the clinical evaluation of combined therapies for gastric cancers. (dovepress.com)
  • Effects of Ganoderma lucidum spores on HepG2 cells proliferation and growth cycle. (springer.com)
  • Id2a-dependent defects in retinoblast proliferation lead to microphthalmia and to an absence of nearly all differentiated inner and outer nuclear layer cell types. (biologists.com)
  • Similarities between acetaminophen- and estradiol-induced proliferation of cultured, estrogen-responsive breast cancer cells. (cdc.gov)
  • this moiety appears to influence estradiol- and xenoestrogen-induced ER+ cell proliferation. (cdc.gov)
  • To this end, effects of p-, m-, and o-acetamidophenol on ER+ (MCF7 and T47D) cell proliferation and on % cells in S (DNA synthesis) phase of the cell cycle were determined. (cdc.gov)
  • These data suggest the p-phenol moiety is important in both estradiol- and acetaminophen-induced ER+ cell proliferation. (cdc.gov)
  • The second goal of this research was to establish if estrogen receptor pathways are involved in mediating the acetaminophen induced proliferation of ER+ cells. (cdc.gov)
  • Effects of antiestrogens on acetaminophen-induced proliferation were assessed by flow cytometry (0/0 S phase cells) and by cell counting. (cdc.gov)
  • In MCF7 and 1470 cells (but not in MDA MB-231 cells), both antiestrogens inhibited proliferation induced by acetaminophen and by estradiol. (cdc.gov)
  • Furthermore, estrogen receptors may directly or indirectly mediate the acetaminophen induced proliferation of ER+ cells. (cdc.gov)
  • Proliferation-linked shifts in eligibility of T-cell progenitors, population dynamics, DN1 cells to undergo specification thus control kinetics of T-cell generation. (lu.se)
  • In response to mito- pendence between the key mecha- gens, cell proliferation is triggered by nistic characteristics. (who.int)
  • H - Ras cell proliferation are also linked with haematopoietic cel s. (who.int)
  • ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)
  • Our course aims to provide a comprehensive understanding of the underlying principles of flow cytometry, while also emphasising practical considerations related to the most frequently used assays. (embl.org)
  • Scientific Methods: In vitro growth inhibition assays were completed with AHCC alone and in combination with PLD in panel of human cancer cell lines and findings confirmed in vivo in an ovarian cancer xenograft mouse model. (researchgate.net)
  • B.A. Osborne, S.W. Smith, Z.-G. Liu, K.A. McLaughlin, and L.M. Schwartz, Transient Transfection Assays to Examine the Requirement of Putative Cell Death Genes. (nhbs.com)
  • In vitro assays for measuring the cytotoxic capacity of immune cells have been a staple in laboratories for many years. (jove.com)
  • These assays have been used to determine the ability of T cells, NK cells, or any other immune cell to kill target cells in an antigen-specific or -nonspecific manner. (jove.com)
  • These in vitro assays involve the mixing of different cell populations, as well as using a relatively low number of both effector and target cells. (jove.com)
  • With the addition of these new formats, more complex assays can be developed to look at apoptosis within heterogeneous cell subsets. (bdbiosciences.com)
  • Collectively, both arms of this Module add scientific value to the work of vision scientists by reducing the time and effort required for basic cell culture assays such as viability and cell cycle assessments. (utsouthwestern.edu)
  • and a Nexcelom Cellometer for rapid determination of cell number, cell viability, transfection efficiency, and cell cycle assays. (utsouthwestern.edu)
  • It consists of 4 fluorescent channels (UV to far red) and has extensive applications including bright field cell counting, wound-healing and cell growth assays, cell line development, induced pluripotent stem cell (iPSC) reprogramming, 3D invasion and migration modeling, immune-oncology, virology, and fluorescent-based assays. (utsouthwestern.edu)
  • Researchers are continuously more engaged in high-parameter flow cytometry assays, analyzing twenty or more markers at high throughput, for deeper insights into the immune network of the host organism. (biolegend.com)
  • Using loss-of-function and overexpression assays in zebrafish, we demonstrate that Id2a levels modulate retinoblast cell cycle kinetics and thereby influence neuron and glia formation in the retina. (biologists.com)
  • Prior basic working knowledge of flow cytometry, data acquisition or analysis software (DIVA + FlowJo) is of advantage, but not strictly a must. (embl.org)
  • It is a must for everyone interested in basic and more specific aspects of flow cytometry techniques. (embl.org)
  • Are you interested in gaining a broad understanding of different aspects of flow cytometry - from basic concepts to common applications and the planning of experiments? (lu.se)
  • The course aims to provide a broad understanding of different aspects of flow cytometry, including experimental design, sample material handling, analysis of flow cytometry data, as well as file and image handling prior to research publication. (lu.se)
  • Housed in the IDDRC Stem Cell Core Facility, this core offers cost effective, state-of-the-art flow cytometry and cell sorting services. (childrenshospital.org)
  • This course is a co-arrangement between the Research School in Stem Cell Biology, the Lund University Neuroscience Graduate School, the StemTherapy FACS Core Facility at Lund Stem Cell Center, and the MultiPark Cellomics and Flow Cytometry Core Facility. (lu.se)
  • Similar cell cycle compartments are also recognized by multiparameter analysis that includes measurement of expression of cyclin D1, cyclin E, cyclin A and cyclin B1, each in relation to DNA content Concurrent measurement of DNA content and of incorporation of DNA precursor 5-bromo-2'-deoxyuridine (BrdU) by flow cytometry is an especially useful assay, that has been widely used in analysis of the cell cycle in vitro and in vivo. (wikipedia.org)
  • Furthermore, Tan IIA-induced apoptosis and mitochondrial membrane potential changes were also further demonstrated by DNA fragmentation, single-cell gel electrophoresis assay (SCGE), and flow cytometry methods. (hindawi.com)
  • 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, acridine orange, and monodansylcadaverine staining and flow cytometry were performed. (hindawi.com)
  • First, the sensitivity and IC 50 values of chidamide and bortezomib in several gastric cancer cell lines (MGC-803, BGC-823, SGC-7901, and MKN45) were measured using the CCK-8 assay. (dovepress.com)
  • Thus, having an assay in which the user can easily integrate any number of different effector cells, target cells, and/or response modifiers into the experimental design can serve as a valuable means of quickly assessing the cytotoxic capacity of effector cells and/or the responsiveness of the target cell. (jove.com)
  • Thus, one necessity of the assay is to label the target cells in a manner that can easily be detected and quantitated, allowing the user to then determine the 'percent specific lysis' mediated by the effector cells. (jove.com)
  • The short labeling and total assay times reduces the potential for significant changes in the number and/or phenotype of the target cells, which could influence the outcome of the assay. (jove.com)
  • As with any assay examining the function of immune cells in vitro , there are a number of important considerations to consider improving the performance of the experiment. (jove.com)
  • Khz cytotoxicity was measured using an MTT assay and the mitochondrial membrane potential (MMP)-related, calcium-induced generation of reactive oxygen species (ROS) in A549 cells was measured by flow cytometry. (springer.com)
  • The aim of the present investigation was to elucidate the cellular mechanisms whereby Tanshinone IIA (Tan IIA) leads to cell cycle arrest and apoptosis in vitro in keratinocytes, the target cells in psoriasis. (hindawi.com)
  • We investigated the effects of various concentrations of Tan IIA (5-10 ÎĽ g/mL) on mouse keratinocytes and human HaCat cells in vitro to confirm this hypothesis. (hindawi.com)
  • Find out more information on our sandwich ELISA options and protocols for specific targets or see how you can analyze individual cells that secrete a particular protein in vitro using our ELISPOT immunoassays. (bdbiosciences.com)
  • The aim of this paper is to analyse sunitinib malate in vitro ability to enhance cisplatin cytotoxicity in T24, 5637, and HT1376 human urinary bladder-cancer cell lines. (hindawi.com)
  • This investigation aims to analyse the in vitro effects of cisplatin and sunitinib malate in isolation and in combination, on one human nonmuscle invasive urinary bladder-cancer cell line (5637) and on two human muscle-invasive urinary bladder-cancer cell lines (T24 and HT1376). (hindawi.com)
  • This technology is used in pharmaceutical research for drug discovery, stem cell research, and in vitro toxicity testing studies. (grandviewresearch.com)
  • Investigating FlowSight® imaging flow cytometry as a platform to assess chemically induced micronuclei using human lymphoblastoid cells in vitro. (swansea.ac.uk)
  • Stem cell senescence in the in vitro expansion stage is an essential concern in clinical procedures of cell therapy. (magiran.com)
  • Flavopiriol blocks the ATP-binding site of cyclin-dependent cinases and leads to a G1- respectively G2-cellcycle arrest in vitro. (uni-marburg.de)
  • This study supports evidence in literature pointing to a certain activity of these drugs and at the same time emphasizes the different sensibility of human ovarian cancer cell lines to targeted therapeutics in vitro. (uni-marburg.de)
  • A.J. McGahon, S.J. Martin, R.P. Bissonnette, A. Mahboubi, Y. Shi, R.J. Mogil, W.K. Nishioka, and D.R. Green, The End of the (Cell) Line: Methods for the Study of Apoptosis In Vitro. (nhbs.com)
  • We tested whether natural killer cells expanded by co-culture with K562 cells transfected with 41BBL and membrane-bound interleukin-15 could kill myeloma cells with a high-risk gene expression profile in vitro and in a unique model which recapitulates human myeloma. (haematologica.org)
  • The incorporation of a flow cytometer and a cell sorter also reduces the cost associated with recurrent use of the main flow cytometry facility on campus. (utsouthwestern.edu)
  • Explore how to maximize your multicolor panel experiment, perform multiomic analysis of hundreds of genes and proteins at a single-cell level, and take advantage of our instruments and reagents for your applications. (bdbiosciences.com)
  • Find tips on how to combine the power and capabilities of our flow cytometry instruments, reagents and software and examine sample data to understand their performance. (bdbiosciences.com)
  • Learn how the BD single-cell multiomics platform, BD Rhapsody™ System, which includes instrument, reagents and bioinformatics solutions, facilitates in-depth analyses of research in immunology, oncology and metabolic diseases. (bdbiosciences.com)
  • P.J. Fraker, L.E. King, D. Lill-Elghanian, and W.G. Telford, Quantification of Apoptotic Events in Pure and Heterogeneous Populations of Cells using the Flow Cytometer. (nhbs.com)
  • Finally, data analysis is dependent on the inclusion of positive and negative control target cell populations. (jove.com)
  • The populations of cells that are stained with annexin V only represent the apoptotic cell populations. (bdbiosciences.com)
  • The flow cytometer is equipped with multiple lasers for analysis of multi-fluorescent labelled cell populations and acoustical focusing for improved cell separations. (utsouthwestern.edu)
  • Find out how to take advantage of antibodies conjugated to magnetic particles for positive and negative selection and pre-enrichment prior to cell sorting. (bdbiosciences.com)
  • These antibodies are available in a variety of formats and can be used for flow cytometry, imaging, ELISA and western blot. (bdbiosciences.com)
  • Flow cytometry (CMF) is a technology of multiparametric cell analysis for detection and identification of molecules and cell structures using fluorescent markers and conjugated antibodies. (csic.es)
  • During a typical flow cytometry experiment, samples are stained using fluorophore-labeled antibodies that recognize and bind specific cell surface or intracellular markers, or a variety of fluorescent chemical probes. (biolegend.com)
  • Cell cycle anomalies revealed on the DNA content frequency histogram are often observed after different types of cell damage, for example such DNA damage that interrupts the cell cycle progression at certain checkpoints. (wikipedia.org)
  • Such an arrest of the cell cycle progression can lead either to an effective DNA repair, which may prevent transformation of normal into a cancer cell (carcinogenesis), or to cell death, often by the mode of apoptosis. (wikipedia.org)
  • S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (cancerindex.org)
  • EIF5B (Eukaryotic Translation Initiation Factor 5B) is a conserved eukaryotic translation factor that mediates association of the 40S and 60S ribosomal subunits during eukaryotic translation initiation, and modulates the cell cycle progression by regulating the translation of upstream open reading frames (uORF)-containing mRNAs such as p27 and p21 [ 3 ]. (techscience.com)
  • These data suggest that EIF5B functions as an oncogene that promotes cancer cell growth, survival, and progression. (techscience.com)
  • Id2a-deficient retinas possess increased numbers of cells occupying S phase, at the expense of mitotic cells, and kinetic analyses demonstrate that Id2a is required for S-phase progression and/or the transition from S to M phase. (biologists.com)
  • Mosaic analyses reveal that Id2a function in facilitating both cell cycle progression and neuronal differentiation in the retina is non-cell-autonomous, suggesting that Id2a functions upstream of the extrinsic pathways that regulate retinogenesis. (biologists.com)
  • Deep RNA-DNA, RNA-protein interaction studies, and phenotype rescue analyses reveal that LETR1 is a nuclear trans-acting lncRNA modulating, via key epigenetic factors, the expression of essential target genes, including KLF4 and SEMA3C , governing the growth and migratory ability of LECs. (nature.com)
  • The latter should be based on chromosome banding analysis as well as on molecular genetic analyses, such as "next-generation sequencing" of relevant genes supplemented with fluorescence in situ hybridization and/or reverse transcription polymerase chain reactions analyses when needed. (cancercentrum.se)
  • Furthermore, the cell cycle regulatory genes p16 INK4a and p14 ARF are noted to be altered in lesions of proliferative verrucous leukoplakia. (medscape.com)
  • Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)
  • Below you can see some examples of the infrastructure for research on genes and cells, available for researchers at Lund University. (lu.se)
  • In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)
  • During EMT, cells will undergo transformation from epithelial phenotype to mesenchymal phenotype ( 14 ) and many characteristics of cells will change including loss of cell-cell adhesion and acquisition of aggressive and metastatic ability ( 15 ). (spandidos-publications.com)
  • Furthermore, EIF5B knockdown suppressed epithelial-mesenchymal transition (EMT) and the cancer stem cell (CSC) phenotype. (techscience.com)
  • Flow cytometry, which first began to develop in the 1950s, is a technology for characterizing the phenotype and functionality of individual cells within a heterogeneous population. (biolegend.com)
  • The TAM receptor and ligand expression was evaluated in LMS cell lines and 358 sarcoma samples by either gene expression or immunohistochemistry. (nature.com)
  • Find an array of useful tools for your immunohistochemistry (IHC) analysis. (bdbiosciences.com)
  • In certain circumstances, when a lymph node is not easily accessible for excisional or incisional biopsy, a combination of core biopsy and FNA biopsies in conjunction with appropriate ancillary techniques for the differential diagnosis (immunohistochemistry, flow cytometry, PCR for IGHV and TCR gene rearrangements, karyotype, and fluorescence in situ hybridization [FISH] for major translocations) may be sufficient for diagnosis. (medscape.com)
  • However, the incorporation of 5-ethynyl-2'-deoxyuridine (EdU), the precursor whose detection offers certain advantages over BrdU, has now become the preferred methodology do detect DNA replicating (S-phase) cells. (wikipedia.org)
  • Before analysis, the cells are usually permeabilised and treated with a fluorescent dye that stains DNA quantitatively, such as propidium iodide (PI) or 4,6-diamidino-2-phenylindole (DAPI). (wikipedia.org)
  • Most fluorescent DNA dyes (one of exceptions is Hoechst 33342) are not plasma membrane permeant, that is, unable to pass through an intact cell membrane. (wikipedia.org)
  • In this study, we investigated the potential mechanism underlying the anti- C. albicans effects of AMP-17 using flow cytometry, transmission electron microscopy, fluorescent probes, fluorescence microplate reader, and confocal laser microscopy. (researchsquare.com)
  • A high-speed sorter is available for a wide range of applications including flow karyotyping, chromosome sorting, cell cycle analysis, stem cell isolation, green fluorescent protein (GFP) containing cell sorting, and immunocytochemistry. (childrenshospital.org)
  • Alternatively, the cells themselves may be engineered to tie the expression of a protein of interest to a reporter such as Green Fluorescent Protein ( GFP ). (biolegend.com)
  • Critically, as flow cytometry has advanced, the number of fluorescent readouts that can be measured simultaneously has increased, along with the sample processing speed. (biolegend.com)
  • Chronic myeloid leukemia (CML) results from BCR-ABL oncogene, which blocks CML cells differentiation and protects these cells from apoptosis. (jcancer.org)
  • Mechanistically, the cell differentiation mediated by JOA may be originated from the inhibition of BCR-ABL/c-MYC signaling in CML cells expressing wild-type BCR-ABL and BCR-ABL-T315I. (jcancer.org)
  • JOA displayed the activity of inhibiting the BCR-ABL and promoted differentiation of not only imatinib -sensitive but also imatinib -resistant cells with BCR-ABL mutation, which could become a potent lead compound to overcome the imatinib -resistant induced by inhibitors of BCR-ABL tyrosine kinase in CML therapy. (jcancer.org)
  • Histone deacetylase (HDAC) plays a pivotal role in regulating the level of cellular DNA acetylation and participate in cell growth, differentiation, and apoptosis. (dovepress.com)
  • Within the B-cell and T-cell categories, two subdivisions are recognized: precursor neoplasms, which correspond to the earliest stages of differentiation, and more mature differentiated neoplasms. (medscape.com)
  • An arrest of cells in G0 or G1 is often seen as a result of lack of nutrients (growth factors), for example after serum deprivation. (wikipedia.org)
  • A cell cycle arrest in early S-phase and in G 0 /G 1 -phase was also found after exposure to cisplatin and sunitinib malate, in isolation, respectively. (hindawi.com)
  • Moreover, cell cycle arrest at G0/G1 phase was triggered by decreased LAPTM5 as well, which could lead to delayed BCa cell growth. (spandidos-publications.com)
  • The combined treatment of Flavopiriol and Lonafarnib in BG1-cells showed a slightly increased cell cycle arrest in the subG1-fraction in selected concentrations. (uni-marburg.de)
  • Furthermore, in cultured Fanconi anemia cells, cell cycle arrest in gap 2/mitosis (G2/M) occurs at lower concentrations of clastogens than in normal cells. (medscape.com)
  • The cellular DNA content of individual cells is often plotted as their frequency histogram to provide information about relative frequency (percentage) of cells in the major phases of the cell cycle. (wikipedia.org)
  • Multiparameter analysis of the cell cycle includes, in addition to measurement of cellular DNA content, other cell cycle related constituents/features. (wikipedia.org)
  • The concurrent measurement of cellular DNA and RNA content, or DNA susceptibility to denaturation at low pH using the metachromatic dye acridine orange, reveals the G1Q, G1A, and G1B cell cycle compartments and also makes it possible to discriminate between S, G2 and mitotic cells. (wikipedia.org)
  • Specific areas of interest include understanding the mechanisms, which allow cancer cells to develop resistance to chemotherapy at the cellular and molecular levels. (sc.edu)
  • In zebrafishes, forced expression of rat lcn2 gene increased the number and thickness of cellular processes in GFAP-expressing radial glia cells, suggesting that lcn2 expression in glia cells plays an important role in vivo . (jneurosci.org)
  • Radioactivity - especially, chromium 51 ( 51 Cr) in the form of Na 2 51 CrO 4 - is an inexpensive way to rapidly and nonspecifically label cellular proteins within the target cells (1). (jove.com)
  • Upon the loss of membrane integrity of the target cells as a result of the cytotoxic activity of the effector cells, the 51 Cr-labelled cellular proteins within the target cells are released into the culture supernatant, becoming available for quantitation. (jove.com)
  • This leads to the loss of cellular structure and function, and ultimately results in cell death. (bdbiosciences.com)
  • Inclusion of tunable resistance pulse sensing for the real time analysis of cellular microvesicles adds an additional layer of innovation. (utsouthwestern.edu)
  • Cellular receptors and targeted cells for these viruses are unknown and, to date, novel astroviruses have not been culturable. (cdc.gov)
  • [ 8 ] Tumor growth factor-alpha is a potent mitogenic polypeptide expressed by epithelial cells under physiologic conditions and by activated macrophages and eosinophils in certain pathologic conditions. (medscape.com)
  • By assaying their anti-proliferative and anti-apoptotic effects using human promyelocytic HL-60 cells, we found that the ethanolic extract of I'm-Yunity-Too was more active in inducing cell death compared to I'm-Yunity, based on measured changes in the expression of caspase 3 and Bax. (spandidos-publications.com)
  • In the glioma cells, EIF5B promotes cell survival by enhancing the translation of several IRES-containing mRNAs including those encoding anti-apoptotic proteins such as XIAP and Bcl-xL [ 4 ]. (techscience.com)
  • L.T. Bemis, F.J. Geske, and R. Strange, Use of the Yeast Two-Hybrid System for Identifying the Cascade of Protein Interactions Resulting in Apoptotic Cell Death. (nhbs.com)
  • In the final stages of the process, dying cells become fragmented into apoptotic bodies and consequently are eliminated by phagocytic cells without significant inflammatory damage to surrounding cells. (bdbiosciences.com)
  • Changes in the plasma membrane are one of the characteristics of the apoptotic process detected in living cells. (bdbiosciences.com)
  • Since intracellular annexin V is also exposed if the plasma membrane is compromised, a membrane-impermeant dye such as 7-AAD is commonly used to distinguish between apoptotic and dead cells to exclude the dead cells. (bdbiosciences.com)
  • Flow cytometric estimation of the apoptotic marker CD95 in peripheral neutrophils, lymphocytes and monocytes was done for 18 infants with non-oedematous protein energy malnutrition (PEM) and 12 oedematous ones, on hospital admission and after supervised nutritional rehabilitation, and compared with 12 matched controls. (who.int)
  • High-risk MDS cases had a significantly higher percentage of apoptotic CD34+ cells and anti-apoptotic survivin+ cells than controls, particularly for trisomy 8 cases. (who.int)
  • Trisomy 8+ cells showed a significant positive correlation with apoptotic CD34+ cells and capacity for colony formation. (who.int)
  • Our results suggest that although trisomy 8 cells are in a pro-apoptotic state, they are checked by the enhanced expression of anti-apoptotic signals which provide them with their proliferative advantage. (who.int)
  • The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)
  • njoyed the course a lot and learned what I was looking for: an opportunity to learn the principles of flow cytometry that are important for planning and performing experiments. (embl.org)
  • The course covers the basics, principles and limitations for the most common methods for multivariate statistics (analysis of variance, principal component analysis and partial least squares regression), experimental design (super-saturated and factorial designs) and method validation with a focus on practical use. (lu.se)
  • Through a combination of theoretical instruction and hands-on training, students will gain the knowledge and skills necessary to effectively design, execute, and interpret flow cytometry experiments. (embl.org)
  • Finally, the inhibitory effect of the combined chidamide and bortezomib treatment on MGC-803 cells was verified in vivo through tumor formation experiments in nude mice. (dovepress.com)
  • The Cell Phenotyping arm of this module is designed to provide technical expertise and assistance with ongoing cell phenotyping experiments. (utsouthwestern.edu)
  • The cell sorter has 4 lasers providing flexibility for experiments with up to 6 colors and with multiple sort collection formats. (utsouthwestern.edu)
  • FACS experiments: planning, implementation and analysis of flow cytometry data. (lu.se)
  • Iron and BIM (Bcl-2-interacting mediator of cell death) proteins were involved in the cytotoxic sensitization process. (jneurosci.org)
  • Active caspases can then cleave many proteins, including poly-ADP ribose polymerase (PARP), other caspases and other protein substrates en masse. (bdbiosciences.com)
  • Flow cytometry techniques have undergone significant development in recent years in their ability to analyse thousands of cells per second and provide statistical information instantly. (csic.es)
  • Flowcytometry Solutions (FlowSols) is a flow cytometry consulting company, which aims at providing complete solution to the researchers and clinicians on flow cytometry. (flowsols.com)
  • The goal of the Cell Culture and Cell Phenotyping Module is to streamline cell culture and phenotyping analyses for vision researchers. (utsouthwestern.edu)
  • The Flow Cytometry Unit provides scientific and technological support to CNB research groups,as well as to researchers from the public and private sectors. (csic.es)
  • The RNA and DNA content of large numbers of individual cells was determined by supravital staining with acridine orange. (rupress.org)
  • Cell cycle analysis was first described in 1969 at Los Alamos Scientific Laboratory by a group from the University of California using the Feulgen staining technique. (wikipedia.org)
  • The first protocol for cell cycle analysis using propidium iodide staining was presented in 1975 by Awtar Krishan from Harvard Medical School and is still widely cited today. (wikipedia.org)
  • Unless staining is performed using Hoechst 33342, the first step in preparing cells for cell cycle analysis is permeabilisation of the cells' plasma membranes. (wikipedia.org)
  • Permeabilisation is therefore crucial for the success of the next step, the staining of the cells. (wikipedia.org)
  • Prior to (or during the staining step) the cells are often treated with RNase A to remove RNAs. (wikipedia.org)
  • S.A. Ben-Sasson, Y. Sherman, and Y. Gavrieli, Identification of Dying Cells--In Situ Staining. (nhbs.com)
  • Chronic myeloid leukemia (CML) is a clonal expansion of the progenitor hematopoietic stem cells arising from the existence of the fusion BCR-ABL oncogene, that represents 15%-20% of the newly diagnosed cases of leukemia patients [ 1 - 2 ]. (jcancer.org)
  • Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
  • LAPMT5 is a lysosomal membrane protein preferentially expressed in immune cells ( 5 , 6 ) and hematopoietic cells ( 7 ), having a close interaction with the Nedd4 ( 8 ), a member of the E3 ubiquitin ligases family ( 8 ). (spandidos-publications.com)
  • Our investigation indicates that most of these studies examined endothelial progenitor cells, hematopoietic stem cells, mesenchymal stem cells, adipose-derived stem cells, and a few others were about less-discussed types of stem cells such as cardiac stem cells, myeloblasts, and induced pluripotent stem cells. (magiran.com)
  • Hematopoietic stem cell transplantation (bone marrow, cord blood, or peripheral blood stem cells) may cure aplastic anemia and prevent myelodysplastic syndrome or leukemia. (medscape.com)
  • The single-cell transcriptome and chromatin accessibility datasets of peripheral blood mononuclear cells in Chinese Holstein cattle. (usda.gov)
  • Single-cell transcriptomic and chromatin accessibility analyses of dairy cattle peripheral blood mononuclear cells and their responses to lipopolysaccharide. (usda.gov)
  • 1 Significant advances have been made by combining novel agents with autologous peripheral blood stem cell transplantation which allows for long-term disease-free survival in the majority of transplant-eligible patients. (haematologica.org)
  • The cells in G1Q are quiescent, temporarily withdrawn from the cell cycle (also identifiable as G0), the G1A are in the growth phase while G1B are the cells just prior entering S, with their growth (RNA and protein content, size) similar to that of the cells initiating DNA replication. (wikipedia.org)
  • Perivascular adipose tissue-derived visfatin is a vascular smooth muscle cell growth factor: role of nicotinamide mononucleotide. (thieme-connect.com)
  • It is also responsible for inhibition of vascular endothelial growth factors receptors, plateletderived growth factor receptors, and stem cell factor receptor [ 8 , 9 ]. (hindawi.com)
  • The France flow cytometry market is expected to register growth during the forecast period owing to, technical development within the field of flow cytometry. (grandviewresearch.com)
  • In addition, increasing incidence of Cancer and HIV/AIDS, usage of flow cytometry in clinical trials, advent of application-specific flow cytometry products are further expected to provide growth platform to this market. (grandviewresearch.com)
  • Although, non-psychoactive cannabidiol (CBD) and cannabigerol (CBG) display antineoplastic effects by repressing tumor growth and angiogenesis both in cell line and animal models, their use as chemotherapeutic agents is awaiting further investigation. (frontiersin.org)
  • Furthermore, we established a BCa cell model with downregulated LAPTM5, revealing a significantly delayed growth rate in the BCa cells with knockdown of LAPTM5. (spandidos-publications.com)
  • These mice are devoid of endogenous natural killer and T-cell activity and were used to determine whether adoptively transferred expanded natural killer cells could inhibit myeloma growth and myeloma-associated bone destruction. (haematologica.org)
  • Adoptive transfer of expanded natural killer cells inhibited the growth of established OPM2 and high-risk primary myeloma tumors grown in the murine model. (haematologica.org)
  • The transferred, expanded natural killer cells proliferated in vivo in an interleukin-2 dose-dependent fashion, persisted up to 4 weeks, were readily detectable in the human bone, inhibited myeloma growth and protected bone from myeloma-induced osteolysis. (haematologica.org)
  • Apoptosis is an organized process that signals cells to self-destruct for cell renewal or to control aberrant cell growth. (bdbiosciences.com)
  • Primig, M.: Integrated RNA- and protein profiling of fermentation and respiration in diploid budding yeast provides insight into nutrient control of cell growth and development. (tu-dresden.de)
  • Khz, a naturally occurring compound derived from the fusion of Ganoderma lucidum and Polyporus umbellatus mycelia, inhibits the growth of cancer cells. (springer.com)
  • Increased expression of immunoreactive tumor growth factor-alpha has been noted in lesions of both proliferative verrucous leukoplakia and oral squamous cell cancer, but not in healthy oral mucosa. (medscape.com)
  • Dr. Lim has more than 18 years of experience in cell sorting, cell cycle analysis, apoptosis and immunophenotyping. (sc.edu)
  • It is used for cell counting & sorting, protein engineering, and detection of the biomarker by passing them by an electronic detection equipment and suspended cells in a fluid stream. (grandviewresearch.com)
  • Flow sorting is an extension of this technology, by which any cell or object measured can be separated selectively from the suspension based on properties measured in the flow stream. (csic.es)
  • Flow cytometric analysis of archived lesions of proliferative verrucous leukoplakia has shown evidence of aneuploid cell lines, with DNA indexes remaining constant throughout the course of disease in most cases. (medscape.com)
  • All patients can evolve into trisomy 8+ MDS [12], cells, since acquiring adequate samples diagnosed with MDS have a reduced in which 8+ appears to confer a favour- for flow cytometric analysis renders the life expectancy compared with age- able prognosis [13]. (who.int)
  • Moreover, some studies demonstrated that LAPTM5 was highly expressed in malignant B lymphomas and involved in B cell malignancies ( 10 ), involving in negative regulation of cell surface T and B cell receptor by promoting lysosome degradation ( 6 ). (spandidos-publications.com)
  • Multiple myeloma (MM) is a malignant plasma cell disorder with debilitating symptoms related to anemia, immunosuppression, bone destruction, and renal failure. (haematologica.org)
  • The risk is increased 1000-fold for squamous cell carcinoma, basal cell carcinoma, malignant melanoma, and fibrosarcoma and is increased 10-fold to 20-fold for other tumors. (medscape.com)
  • Proliferative verrucous leukoplakia (PVL) is an uncommon form of progressive multifocal leukoplakia with a high rate of malignant transformation to either squamous cell cancer or verrucous carcinoma and a high probability of recurrence. (medscape.com)
  • [ 9 ] More recently, it has been suggested that a combination of image-based DNA ploidy analysis and evidence of dysplasia on histology may be useful in predicting which lesions are likely to undergo malignant transformation. (medscape.com)
  • Together, our study provides several lines of evidence supporting the intriguing concept that every cell type expresses precise lncRNA signatures to control lineage-specific regulatory programs. (nature.com)
  • In particular, the FANTOM (Functional Annotation of the Mammalian Genome) consortium pioneered the discovery of the noncoding RNA world by providing, through Cap Analysis of Gene Expression (CAGE-Seq), the first evidence that large portions of our genome are transcribed, producing a multitude of sense and antisense transcripts 12 . (nature.com)
  • in BG1-cells evidence for a synergism of Flavopiridol and Lonafarnib was found. (uni-marburg.de)
  • Further, AMP-17 treatment resulted in an increase in reactive oxygen species (ROS) levels, depolarization of the mitochondrial membrane potential (MMP), and changes in the cell cycle, leading to the apoptosis and necrosis, which ultimately contributed to the death of C. albicans cells. (researchsquare.com)
  • Jacobson MD. Reactive oxygen species and programmed cell death. (springer.com)
  • Miyajima, A, Nakashima J, Yoshioka K. Role of reactive oxygen species in cis-dichlorodiammineplatium-induced cytotoxicity on bladder cancer cells. (springer.com)
  • Storage of bone marrow or blood leukemic cells in biobanks is strongly recommended. (cancercentrum.se)
  • 15]. The percentage of bone common of which are trisomy 8, mono- marrow blast cells for estimation of the Myelodysplastic syndrome (MDS) is somy 7 and 5q- [11]. (who.int)
  • BD Biosciences tools use multiple methodologies including flow cytometry , bioimaging and microscopy (for live and fixed cell analysis) as well as ELISA , IHC , western blot and spectrofluorometry. (bdbiosciences.com)
  • We studied the cell cycle of lectin-stimulated human lymphocytes, making use of a flow cytometer. (rupress.org)
  • Moreover, inclusion of a high throughput imaging cytometer allows for many of these same comparisons to be made without the need for trypsinization and extensive cell processing. (utsouthwestern.edu)
  • Equipment in this laboratory includes an Attune NxT Flow Cytometer from Life Technologies and a Sony SH800 Cell Sorter. (utsouthwestern.edu)
  • One of the main functions of the cells of the immune system is to remove target cells that have been infected with viruses or have undergone transformation into a tumor cell. (jove.com)
  • It is suggested that curcumin can selectively kill tumor cells through its multifaceted metabolic effects, that culminate in its anti-oxidant and anti-inflammatory activities ( Hewlings and Kalman, 2017 ). (frontiersin.org)
  • In addition to inhibiting pathogens such as bacteria and fungi, AMPs also have varying degrees of killing effects on viruses, tumor cells, and parasites (Falanga et al. (researchsquare.com)
  • Our findings revealed that different genetic backgrounds of HCT116 and HT29 cell lines resulted in divergent responses to the combination treatments. (frontiersin.org)
  • Therefore, based on clinical experience as well as cytotoxic activity against multiple human cancer cells, we hypothesized that induction of apoptosis is the underlying mechanism for the treatment of psoriasis. (hindawi.com)
  • The techniques described facilitate both understanding and regulation of cell death, promising to have immense value in the clinical treatment of cancer. (nhbs.com)
  • Data analysis can be performed on DIVA and FlowJo. (rvc.ac.uk)
  • This FREE sample includes market data points, ranging from trend analyses to market estimates & forecasts. (grandviewresearch.com)
  • Overall, our data suggest that ALKBH6 is required to maintain the integrity of the genome and promote cell survival of pancreatic cancer cells. (frontiersin.org)
  • This core provides projects with assistance in designing integrative genomics studies and in data analysis and interpretation. (childrenshospital.org)
  • It is equipped with Gen5 Reader Control and Data Analysis Software. (utsouthwestern.edu)
  • Accepted: 24 October 2012 cell yield data, our models require that adult DN1 cells divide multiple times before becoming competent to progress into DN2 stage. (lu.se)
  • Stem cell senescence causes different complications. (magiran.com)
  • In addition to the aging phenomenon, stem cell senescence has been investigated in various concepts such as cancer, adverse drug effects, and as a limiting factor in cell therapy. (magiran.com)
  • This manuscript examines protective medicines and supplements which are capable of hindering stem cell senescence. (magiran.com)
  • We searched the databases such as EMBASE, PubMed, and Web of Science with the keywords "stem cell," "progenitor cell," "satellite," "senescence" and excluded the keywords "cancer," "tumor," "malignancy" and "carcinoma" until June 2020. (magiran.com)
  • Epstein-Barr virus in oral proliferative verrucous leukoplakia and squamous cell carcinoma: A preliminary study. (medscape.com)
  • Analysis of the changes of migration and invasion, showed significant reduced LAPTM5 suppressed cell metastasis. (spandidos-publications.com)
  • We propose that lcn2 is an autocrine mediator of reactive astrocytosis based on the multiple roles of lcn2 in the regulation of cell death, morphology, and migration of astrocytes. (jneurosci.org)
  • LCN2 protein induced upregulation of glial fibrillary acidic protein (GFAP), cell migration, and morphological changes similar to characteristic phenotypic changes termed reactive astrocytosis. (jneurosci.org)
  • Our results indicated that lcn2 is critical for cell death sensitization, stimulation of cell migration, and morphological changes of reactive astrocytes. (jneurosci.org)
  • Univariate Cox regression analysis showed that Annexin A2 and S100A10 in stromal tissue correlated with shorter overall survival (OS). (cancerindex.org)
  • In particular, the loss of ALKBH6 in human pancreatic cancer cells increases alkylating agent-induced DNA damage and significantly decreases cell survival. (frontiersin.org)
  • Furthermore, in silico analysis from The Cancer Genome Atlas (TCGA) database suggests that overexpression of ALKBH6 provides better survival outcomes in patients with pancreatic cancer. (frontiersin.org)
  • The extremely low 5-year overall survival of patients is, in part, due to pancreatic cancer cells having several mechanisms of resistance to different chemotherapeutic treatments, one of which is their capacity to efficiently repair alkylating agent-induced DNA damage. (frontiersin.org)
  • Epithelial-mesenchymal transition (EMT) is a dynamic process involved in cancer metastasis wherein cancer cells with epithelial characteristics acquire mesenchymal characteristics such as increased motility, invasion, and survival [ 6 ]. (techscience.com)
  • However, the precise role of lcn2 in the cell survival and death has yet to be determined. (jneurosci.org)
  • However, we know less about the biological effects of IL-21 and its signaling mechanisms in leukemia cells and monocytes. (intechopen.com)
  • In this chapter, we will focus on IL-21's biological effects and signaling pathways as well as discuss the potential implications and applications of IL-21 in leukemia cells. (intechopen.com)
  • Young children have acute lymphoblastic leukemia of T-cell origin. (medscape.com)
  • Older children have T-cell leukemia. (medscape.com)
  • Acute myeloid leukemia (AML) is a disease with diverse genetic features of the leukemic cells and with variable outcome. (cancercentrum.se)
  • in BG1-cells combining both drugs led to a rise of necrosis and a reduction of vital cells. (uni-marburg.de)
  • Apoptosis controls the orderly death of damaged cells, whereas necrosis occurs as a result of tissue damage, causing the loss of both damaged and surrounding cells. (bdbiosciences.com)
  • Radio-frequency dose-dependent apoptosis, necrosis and cell death monitored by annexin V-BD Horizon™ V450. (bdbiosciences.com)
  • Radio-frequency dose-dependent apoptosis, necrosis and cell death monitored by annexin V-BD Horizon™ V450 in pancreatic carcinoma cell lines treated with a low dose of cetuximab targeted gold nanoparticles. (bdbiosciences.com)
  • Flow cytometry involves the analysis of the optical and visible light characteristics of a single particle (e.g. cells, nuclei, chromosomes) throughout their passage within a slender, specifically printed liquid stream. (grandviewresearch.com)
  • Recently, we reported that cultured rat cortical astrocytes and glioma C6 cells synthesize melatonin. (nel.edu)
  • The mechanisms influenced by Flaoprirol and Lonafarnib are relevant on ovarian cancer cells. (uni-marburg.de)
  • SKOV-3 and BG1 were used as ovarian cancer cell lines. (uni-marburg.de)
  • Zhao S, Ye G, Fu G, Cheng JX, Yang BB, Peng C. Ganoderma lucidum exerts anti-tumor effects on ovarian cancer cells and enhances their sensitivity to cisplatin. (springer.com)
  • Flow cytometry is also used to detect cancer & immunodeficiency disorders, and in hematology, it is used for organ transplantation studies and in other applications. (grandviewresearch.com)