• Using the human corneal epithelial cell line HCE-T, adhesion to laminin isoforms and fibronectin was determined using a colorimetric adhesion assay. (nih.gov)
  • A nanoscale modular design strategy was employed to synthesize six engineered, recombinant proteins intended to mimic aspects of the extracellular matrix proteins fibronectin, laminin, and elastin as well as the cell-cell adhesive protein neural cell adhesion molecule. (frontiersin.org)
  • As confirmation that ligand density in these engineered systems impacts neuronal cell behavior, we demonstrate that increasing the density of fibronectin-derived RGD ligands on coated surfaces while maintaining uniform protein surface coverage results in enhanced neurite extension of PC-12 cells. (frontiersin.org)
  • Ligands for integrins include fibronectin, vitronectin, collagen and laminin. (biomaterialsusa.com)
  • Previous studies have shown that small beads coated with FN7-10, a four-domain cell adhesion fragment of fibronectin, bind to cell surfaces and translocate rearward. (biologists.com)
  • Compared with controls, a marked reduction in adhesion to fibronectin (20C40%) and increase (40C60%) in adhesion to laminin was observed in the two clones (S8 and S25) demonstrating comparatively high levels of ST6GalNAcV mRNA expression and cell surface 2,6-linked gangliosides (Fig. 4). (biomedigs.org)
  • Altered adhesivity of U373MG cells to fibronectin by modulation of cell surface N-linked glycoconjugates is usually mediated by altered tyrosine phosphorylation of p125FAK (5). (biomedigs.org)
  • After adhesion to fibronectin, however, there was a striking difference in the level of phosphorylation of a 75-kDa protein in all of the transfectants. (biomedigs.org)
  • Actinomycin D distributor Cell adhesion to human fibronectin, laminin, and collagen type I was measured, also as described (5). (biomedigs.org)
  • After adhesion to fibronectin as above, 300 g of cell lysate protein was fractionated on an Agilent 3100 OFFGel Electrophoresis Apparatus by using 24-cm IPG strips, pI 3C10 (GE Healthcare) in urea, thiourea, DTT, glycerol, and the corresponding GE ampholine under conditions recommended by Agilent. (biomedigs.org)
  • To demonstrate differences in human lens epithelial cell adhesion to different intraocular lens biomaterials in vitro and to determine whether these differences can be influenced by coating the intraocular lens surface with commercially available fibronectin. (arvojournals.org)
  • Coating the lenses with fibronectin caused a significant increase in attachment of lens epithelial cells for all three lens types. (arvojournals.org)
  • Cell attachment was more prominent in the acrylic lenses, but the fibronectin coating negated differences in lens type and caused a significant increase in cell attachment across all groups. (arvojournals.org)
  • These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. (wikipedia.org)
  • Physical adsorption isotherms were experimentally determined for these engineered proteins, allowing for direct calculation of the available ligand density present on coated surfaces. (frontiersin.org)
  • The laminin globular (G) domain can be found in one to several copies in various laminin family members, including a large number of extracellular proteins. (embl.de)
  • Laminin G-containing proteins appear to have a wide variety of roles in cell adhesion, signalling, migration, assembly and differentiation. (embl.de)
  • Due to their large surface area and ability to interact with proteins and peptides, graphene oxides offer valuable physiochemical and biological features for biomedical applications and have been successfully employed for optimizing scaffold architectures for a wide range of organs, from the skin to cardiac tissue. (mdpi.com)
  • We describe a novel synergistic effect between substrate-bound hemolymph proteins and laminin. (nature.com)
  • Substrate-bound factor(s) from central nervous system-conditioned media also had a synergistic effect with laminin, suggesting a possible cooperation between humoral proteins and nervous system extracellular matrix. (nature.com)
  • A heterogeneous family of water-soluble structural proteins found in cells of the vertebrate lens. (lookformedical.com)
  • ABSTRACTAbout Cell Culture Protein Surface Coating Cell culture protein surface coating is a procedure in which the cell culture surfaces are coated with proteins or extracellular matrix (ECM) components to enhance the adhesion and proliferation of the cells during in vitro isolation and cultivation. (marketreport.biz)
  • Laminin binding proteins. (am-online.org)
  • The aim of the present study was to functionalize Ti scaffolds with genetically modified elastin-like recombinamers (ELRs), synthetic polymeric proteins containing the elastin epitopes responsible for their mechanical properties and for promoting mesenchymal stem cell (MSC) recruitment, proliferation, and differentiation to ultimately increase scaffold osseointegration. (ibecbarcelona.eu)
  • Whenever an artificial surface comes into contact with blood, proteins are rapidly adsorbed onto its surface. (ibecbarcelona.eu)
  • Moreover, identification of the remaining adsorbed proteins is performed via mass spectroscopy to elucidate their influence on the surface hemocompatibility. (ibecbarcelona.eu)
  • The effect of this polymer on adhesion properties with specific extracellular matrix components was studied using several matrix proteins as substrates for cell attachment. (embrapa.br)
  • It breaks down the anchorage proteins that attach the cell to the culture vessel. (capricorn-scientific.com)
  • There he worked with Dr. Max D. Cooper (Howard Hughes Medical Institute, National Academy of Sciences) and his research focused on cell surface proteins expressed by preB cells that regulate B cell maturation and homing. (ubc.ca)
  • In addition, he identified a number of novel hematopoietic stem cell surface proteins and began analyzing their function. (ubc.ca)
  • His laboratory has followed two primary interests: 1) the transcription factor networks that regulate fate determination in various cells that make blood, and 2) the cell surface proteins expressed by hematopoietic stem cells that and allow them to communicate with their microenvironment. (ubc.ca)
  • In this regard, his lab has identified a novel family of hematopoietic cell surface proteins, called the CD34 family, and shown that these are essential for a number of developmentally important processes. (ubc.ca)
  • After adhesion to laminin, both the qualitative and quantitative pattern of phosphorylated proteins in each of the clones was identical to that observed in control cells (Fig. S4). (biomedigs.org)
  • The integrin tails have no intrinsic kinase activity but rather serve as a site for the docking of various kinases and related adaptor proteins that comprise focal adhesions. (thno.org)
  • It is well known that the adsorption of proteins onto biomaterial surfaces modulates the cellular interaction with these surfaces. (wikigenes.org)
  • Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. (cancer-genetics.org)
  • The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. (cancer-genetics.org)
  • This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. (cancer-genetics.org)
  • Using mass spectrometry, quantitative proteomic analysis and Gene Ontology terms filters, we observed that EVs released by EGFRvIII-transformed cells were enriched for extracellular exosome and focal adhesion related proteins. (cancer-genetics.org)
  • Among them, we validated the association of pro-invasive proteins (CD44, BSG, CD151) with EVs of EGFRvIII expressing glioma cells, and downregulation of exosomal markers (CD81 and CD82) relative to EVs of EGFRvIII-negative cells. (cancer-genetics.org)
  • Nano-flow cytometry revealed that the EV output from individual glioma cell lines was highly heterogeneous, such that only a fraction of vesicles contained specific proteins (including EGFRvIII). (cancer-genetics.org)
  • The way in which where mTOR pathways immediate actin redesigning and cell motion are not realized but may involve a colocalization of S6K1 with actin tension fibers18 aswell as actin redesigning proteins such as for example Rac1. (researchtoactionforum.org)
  • These properties will act mediating the action of cell adhesion proteins, regulating cell behavior and causing tissue regeneration. (bvsalud.org)
  • To occur the adhesion of CAMs to the biomaterial, it is necessary their interaction with some components of extracellular matrix, such as proteoglycans, collagen and proteins 23 . (bvsalud.org)
  • Although the molecular identities of these factors have not been reported, collagen-like peptides have been identified in the material produced by arterial and sheath cells 13 . (nature.com)
  • Coating of glass slides with Entactin-Collagen IV-Laminin attachment (ECL) matrix further enhanced the adhesion of P. multocida. (usda.gov)
  • No significant difference in adhesion to collagen was exhibited. (biomedigs.org)
  • In DNA microarray results, compared to control, CaG treated mouse group showed upregulation of 192 genes including collagen,typeI,alpha1 (Col1a1), consistent with the highly increased in vitro extracellular matrix (ECM) adhesion on collagen 1 and up-regulation of heparanase (Hpse). (biomedcentral.com)
  • Glycosaminoglycan, CaG can strengthen ECM by increasing activity of TIMP-2 and adhesion activity on collagen known to inhibit changes of ECM, leading to tumor cell invasion and progression. (biomedcentral.com)
  • Several molecules are involved in the process of cell adhesion to bone grafts, highlighting the role of integrins, the focal adhesion mechanism, the influence of the collagen matrix, and the activity of alkaline phosphatase in bone matrix formation. (bvsalud.org)
  • They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. (wikipedia.org)
  • The object of this work has been to investigate the formation and function of GalTase-specific adhesions during F9 cell growth and differentiation. (tmc.edu)
  • surface GalTase levels remained constant during differentiation. (tmc.edu)
  • Surface GalTase recognized several surface glycoproteins in a pattern that changes with differentiation. (tmc.edu)
  • The function played by surface GalTase in growth and induction of differentiation was examined. (tmc.edu)
  • Disruption of GalTase adhesion by exposure to anti-GalTase antibodies during this period resulted in extension of the G2 phase, a result similar to that seen with agents known to inhibit growth and induce differentiation. (tmc.edu)
  • Finally, other studies have suggested that a subset of cell adhesion molecules have the capability to induce differentiation in EC cells systems. (tmc.edu)
  • We have determined in F9 cells that dissociating GalTase adhesion by galactosylation of and release of the consociate glycoproteins induces differentiation, as defined by increased laminin synthesis. (tmc.edu)
  • The ability to induce differentiation by surface galactosylation was greatest in cells grown in cultures promoting cell-cell adhesions, relative to cultures with minimal cell-cell interactions. (tmc.edu)
  • Ghasemi-Mobarakeh L, Prabhakaran MP, Tian L, Shamirzaei-Jeshvaghani E, Dehghani L, Ramakrishna S. Structural properties of scaffolds: Crucial parameters towards stem cells differentiation. (wjgnet.com)
  • Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
  • Cell adhesion, proliferation, and colonization were enhanced on those scaffolds functionalized with RGD-ELR, while differentiation was promoted on those with SNA15-ELR. (ibecbarcelona.eu)
  • The combination of both RGD and SNA15 into the same ELR stimulated cell adhesion, proliferation, and differentiation, although at lower levels than those for every single moiety. (ibecbarcelona.eu)
  • Further investigation on the amount and distribution of RGD and SNA15 moieties in ELRs could improve cell adhesion, proliferation, and differentiation compared to the present study. (ibecbarcelona.eu)
  • This is one of the reasons why serum itself - or an animal-free equivalent - is so important for cell growth and differentiation. (capricorn-scientific.com)
  • Expression of cell-surface glycoproteins was evaluated by SNA lectin blot analysis, as described (5), by using the DIG Glycan Differentiation Kit (Roche Molecular Biochemical), exactly as per the manufacturer's recommendations. (biomedigs.org)
  • Integrin-mediated interactions with the extracellular matrix (ECM) are required for the attachment, cytoskeletal organization, mechanosensing, migration, proliferation, differentiation and survival of cells in the context of a multitude of biological processes including fertilization, implantation and embryonic development, immune response, bone resorption and platelet aggregation. (thno.org)
  • Signals emanating from focal adhesions have been shown to promote survival, differentiation and proliferation [ 5 ]. (thno.org)
  • Laminins [ ( PUBMED:2404817 ) ] are the major noncollagenous components of basement membranes that mediate cell adhesion, growth migration, and differentiation. (embl.de)
  • In order to be effective and integrated to the receiving area, the bone graft is required to allow a strong cell adhesion, interacting with several molecules to induce migration, differentiation, and thus the mineralization of the new bone on the graft. (bvsalud.org)
  • Notwithstanding, to achieve the effectiveness and integration of the grafted tissue to the receptor site, it is necessary a strong cell adhesion, so that it demands many molecules interaction to induce cell differentiation and the bone matrix mineralization formed onto the graft. (bvsalud.org)
  • RPSA was initially identified as a laminin binding protein. (wikipedia.org)
  • To the most important virulence factors belong: capsule, C5a peptidase, which inhibits the action of neutrophils, α-C protein, laminin binding protein, and β hemolysin typical for invasive strains. (am-online.org)
  • Increased mRNA expression of a laminin-binding protein in human colon carcinoma: complete sequence of a full-length cDNA encoding the protein. (joplink.net)
  • Indeed, granule cells from the rostral region of perinatal Gpr56 −/− cerebella show loss of adhesion to extracellular matrix molecules of the pial basement membrane. (jneurosci.org)
  • These studies establish a novel role for GPR56 in the adhesion of developing neurons to basal lamina molecules and suggest that this adhesion is critical for maintenance of the pia and proper cerebellar morphogenesis. (jneurosci.org)
  • Consistent with this hypothesis, developing granule cells from the rostral region of Gpr56 −/− cerebella at birth exhibit a specific defect in cell adhesion to extracellular matrix (ECM) molecules of the pial basement membrane (BM) but no defects in migration, proliferation, or process outgrowth. (jneurosci.org)
  • A key component of the ICM niche are the extracellular vesicles (EVs), produced by distinct cell types, that carry and transfer key molecules that regulate target cells and modulate cell renewal or cell fate. (bvsalud.org)
  • Microvessels act as the ultimate barrier between tissue and blood and regulate the exchange of molecules and cells [ 1 ]. (biomedcentral.com)
  • These links are used in cell motility as the actin cytoskeleton generates force pulling the integrin and attached matrix molecules towards the cell center. (biologists.com)
  • Redirecting endogenous antibodies in the bloodstream to tumor cells utilizing artificial molecules is a promising strategy to set off anti-tumor immune responses. (kashbiotech.com)
  • Protein bridges, also called substrate adhesion molecules, have receptors on the cell side, as well as adhesion molecules on the substrate side. (capricorn-scientific.com)
  • Some adhesion molecules are synthesized by the cell, but most of them are derived from serum . (capricorn-scientific.com)
  • Through gene knockout studies he has shown that these molecules act as a type of molecular "Teflon" to make cells more mobile and invasive and also facilitate chemotaxis. (ubc.ca)
  • He has delineated the function of these molecules in diverse set of biological processes including: 1) gut and kidney formation, 2) vascular permeability, 3) mucosal inflammatory disease, 4) stem cell homing and migration, and 5) epithelial tumor progression. (ubc.ca)
  • Integrins are heterodimeric, transmembrane receptors that function as mechanosensors, adhesion molecules and signal transduction platforms in a multitude of biological processes. (thno.org)
  • Cell type and tissue specific alterations in fine GAG structure, which are strictly predetermined [ 8 - 10 ], allow these molecules to modulate with high specificity different cellular processes [ 7 ]. (hindawi.com)
  • PGs, molecules which consist of a protein core that is covalently modified with GAG chains, are distributed both to the ECM "proper" associated with the cell membrane as well as located to intracellular compartment. (hindawi.com)
  • These cell adhesion molecules (CAM) will mediate the contact between two cells or between cells and the extracellular matrix, an essential process to the success of the implant. (bvsalud.org)
  • This paper is a systematic review of the literature on the mechanisms of cell adhesion to bone grafts associated to nanotechnology, describing the importance and the role of those molecules in the adhesion and thus in tissue regeneration. (bvsalud.org)
  • The main reported mechanisms involve cell adhesion molecules (CAMs) and extracellular matrix components. (bvsalud.org)
  • Tissue engineering is a strategy very used to obtain functional repairing through the development of biological substitutes that can restore, keep or substitute damaged tissues or organs 31 , through the combination of scaffolds biocompatible with live cells and/or bioactive molecules 19 . (bvsalud.org)
  • Some of these molecules are glycoproteins expressed on the cell surface, socalled cell adhesion molecules (CAM), which mediate either the contact between two cells or the contact among cells and the extracellular matrix, being therefore of fundamental importance for adhesion 3 . (bvsalud.org)
  • Both recombinant N-domain and C-domain of RPSA bind laminin in vitro, and they bind with similar dissociation constants (300 nM). (wikipedia.org)
  • Therefore, this engineered protein adsorption approach allows for the facile preparation of tunable, quantifiable, and reproducible surfaces for in vitro studies of cell-ligand interactions and for potential application as coatings on neural implants. (frontiersin.org)
  • Jeff has over 20 years' experience in cancer research, including using animal models as well as in vitro invasion and migration systems such as Corning® BioCoat™ Matrigel® Invasion Chambers and Corning FluoroBlok™ Cell Culture Inserts. (cellculturedish.com)
  • In vitro maturation of TPBM over a period of 6 days resulted in gradual increased adhesion of the bacteria. (usda.gov)
  • The blastocyst inner cell mass (ICM) that gives rise to a whole embryo in vivo can be derived and cultured in vitro as embryonic stem cells (ESCs), which retain full developmental potential. (bvsalud.org)
  • We present that Fc-ARM concentrating on folate receptor-α (FR-α) redirects a clinically used antibody combination to FR-α + most cancers cells, leading to most cancers cell lysis by pure killer cells in vitro . (kashbiotech.com)
  • In Vitro Adhesion. (biomedigs.org)
  • There was a significant difference in the degree of lens epithelial cell attachment to the various types of intraocular lenses in vitro. (arvojournals.org)
  • A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
  • We examined the effects of statin drugs on in vitro proliferation, migration and invasion of melanoma cells. (biomedcentral.com)
  • Notably, the addition of hemolymph to laminin substrates accelerated growth cone migration rate over ten-fold. (nature.com)
  • Hilary has extensive experience with cancer cell migration, immune oncology assays and 3D cell culture. (cellculturedish.com)
  • It is also possible to combine Corning 96HTS inserts with the Corning spheroid microplate in order to look at immune cell migration/invasion towards the cancer organoid. (cellculturedish.com)
  • The process of metastasis is the consequence of migration of melanoma cells from the primary lesion, to locoregional and distant body sites via the lymphatic circulation and the bloodstream. (hindawi.com)
  • During migration in embryos, cells must alter their overall adhesiveness to the substrate to allow locomotion. (silverchair.com)
  • We assessed the effect of CD151 loss on cell migration and survival and found that CD151 silencing did not significantly alter (1) EC motility in Transwell migration (supplemental Physique 3A) and wound healing (data not shown) assays and (2) EC viability after being plated on Matrigel immediately (supplemental Figure. (biospraysehatalami.com)
  • Effects on cell migration and invasion were assessed using transwell invasion and migration chambers. (biomedcentral.com)
  • Lovastatin, mevastatin and simvastatin inhibited the growth, cell migration and invasion of HT144, M14 and SK-MEL-28 melanoma cells. (biomedcentral.com)
  • Leukocyte adhesion deficiency type I (LAD I) is a failure to express CD18, which composes the common ß 2 subunit of LFA1 family (ß2 integrins). (medscape.com)
  • Integrins are receptors that facilitate cell-extracellular matrix (ECM) adhesion. (biomaterialsusa.com)
  • Several types of integrins exist, and one cell may have multiple different types on its surface. (biomaterialsusa.com)
  • We provide a complete integrin binding peptide array to help researchers to quickly identify the types of integrins on the cell surface. (biomaterialsusa.com)
  • Integrins are transmembrane receptors that mediate cell adhesion by forming links between the extracellular matrix and cytoskeleton. (biologists.com)
  • Integrins are heterodimeric cell surface receptors found in nearly all metazoan cell types, composed of non-covalently linked α and β subunits. (thno.org)
  • Galactosyltransferase (GalTase) is localized in the Golgi, where it functions in oligosaccharide synthesis, as well as on the cell surface where it serves as a cell adhesion molecule. (tmc.edu)
  • It was subsequently demonstrated to be a cell surface molecule involved in many physiological processes, such as vesicle trafficking. (bvsalud.org)
  • CD86,along with CD80/B7-1.is an important accessory molecule in T cell costimulation via it's interaciton with CD28 and CD152/CTLA4.Since CD86 has rapid kinetics of induction.it is believed to be the major CD28 ligand expressed early in the immune response.it is also found on malignant Hodgkin and Reed Sternberg(HRS) cells in Hodgkin's disease. (researchtoactionforum.org)
  • P-selectin, soluble endothelial protein C receptor, soluble thrombomodulin, plasminogen activator inhibitor 1, ADAMTS-13, von Willebrand factor, tissue factor, soluble intercellular adhesion molecule 1, and vascular cell adhesion molecule 1 were more elevated in LF patients than in controls. (cdc.gov)
  • Endothelial protein C receptor, thrombomodulin, intercellular adhesion molecule 1, plasminogen activator inhibitor 1, D-dimer, and hepatocyte growth factor were higher in fatal than nonfatal LF cases. (cdc.gov)
  • The RPSA gene encodes a multifunctional protein, which is both a ribosomal protein and a high-affinity, non-integrin laminin receptor. (wikipedia.org)
  • To analyze alpha-integrin mediated adhesion of human corneal epithelial cells to placental and EHS laminin isoforms. (nih.gov)
  • alpha-integrin sub-unit modulation of corneal epithelial cell interaction with laminin isoforms was analyzed using immunofluorescence microscopy and adhesion assays incorporating functional blocking antibodies. (nih.gov)
  • Immunofluorescence microscopy reveals that alpha-integrin protein localization patterns are not significantly different in HCE-T interacting with EHS or placental laminin. (nih.gov)
  • However, in short-term assays alpha3 integrin plays a major role, and alpha2 integrin a minor role, in mediating HCE-T adhesion to laminin. (nih.gov)
  • alpha6 integrin does not appear to mediate adhesion to either substrate. (nih.gov)
  • Synthetic peptides from the carboxy-terminal globular domain of the A chain of laminin: their ability to promote cell adhesion and neurite outgrowth, and interact with heparin and the beta 1 integrin subunit. (rupress.org)
  • Antibodies against the beta 1 integrin subunit inhibited HT-1080 cell adhesion and neurite outgrowth on surfaces adsorbed with peptides GD-3 and GD-4. (rupress.org)
  • Furthermore, these results suggest that the sequences comprising peptides GD-3 and GD-4 use an integrin as a receptor, of which the beta 1 integrin subunit is a component for these various cell types. (rupress.org)
  • In addition, integrin binding has been identified as a means of viral entry into cells [ 3 ]. (thno.org)
  • Integrin mediated cellular adhesion is dynamic process that is both indicative of and influenced by cell status. (thno.org)
  • Integrin ligation via cell-to-cell and cell-to-matrix contacts transduces intracellular signaling events in a process called 'outside-in' signaling (Fig. 2 ). (thno.org)
  • It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. (cancer-genetics.org)
  • residues 2,110-3,060) of the A chain of murine-derived laminin has been shown to promote heparin binding, cell adhesion, and neurite outgrowth. (rupress.org)
  • This combination increased outgrowth and branching relative to either laminin or hemolymph alone. (nature.com)
  • Neurite outgrowth can be initiated de novo by experimental application of tension to the cell margin of forebrain neurons placed into culture 8-12 hours earlier, prior to spontaneous neurite outgrowth. (biologists.com)
  • These studies demonstrate that human corneal epithelial cells are capable of rapid adhesion to, and enhanced spreading on, laminin isoforms not characteristically resident in the adult corneal basement membrane. (nih.gov)
  • This characteristic of human corneal epithelium may explain, at least in part, why amniotic membrane transplantation is proving to be clinically useful for human ocular surface reconstruction. (nih.gov)
  • Important biological events associated with plasma membranes, such as signal transduction, cell adhesion, and protein trafficking, are mediated through the membrane microdomains. (mdpi.com)
  • Uvomorulin, lysosome-associated membrane protein-1 (LAMP-1), and laminin were recognized by surface GalTase and are, therefore, potential components in GalTase-specific adhesions. (tmc.edu)
  • Although it does not appear much published work has been done in this area, FluoroBlok inserts are a great choice when one is looking to exclude fluorescent signal generated by cells on the apical surface of the membrane. (cellculturedish.com)
  • The expression of biomarkers can be detected intracellularly or on the cell membrane of melanoma cells or noncancer cells in association with the melanoma. (hindawi.com)
  • An 11-kDa protein associated with the outer membrane of many cells including lymphocytes. (lookformedical.com)
  • Endothelial cells, which form the inner lining of microvessels, create this semipermeable barrier via a combination of cellular junctions, their glycocalyx, and the basement membrane that consists of vessel-specific extracellular matrix (ECM) [ 2 ]. (biomedcentral.com)
  • Embedded within the basement membrane and in direct contact with endothelial cells are pericytes, which are crucial for microvessel maturation, maintenance, as well as proper function. (biomedcentral.com)
  • Trypsin does not act as a reversal of the cell-substrate bond, but rather leads to rounding of the cells and acts on the membrane-cytoskeletal complex. (capricorn-scientific.com)
  • Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. (joplink.net)
  • GAGs bound into PGs are located to the extracellular matrix, basal membrane, and cell surface [ 7 ]. (hindawi.com)
  • CD11a/CD18 (LFA-1) expressed on lymphocytes is known to play an important role in lymphocyte trafficking (adhesion to vascular endothelium), as well as interactions to antigen presenting cells (APC). (medscape.com)
  • data demonstrate that Compact disc151 keeps vascular balance by marketing endothelial cell adhesions specifically cell-cell adhesion and confining cytoskeletal stress. (biospraysehatalami.com)
  • In the following hours both cells put together networks of cable structures to a similar extent suggesting that CD151 is not required for initial EC patterning into vascular structures (supplemental Videos). (biospraysehatalami.com)
  • Pathologic changes seen on autopsy lack major cell and tissue injury but include signs of pleural effusion, pulmonary edema, ascites, and gastrointestinal mucosa bleeding ( 5 , 6 ), all indications of systemic vascular leakage. (cdc.gov)
  • In this study, existing polyacrylamide gel polymerization methods were modified into a novel method for making substrates capable of engaging specific cell-adhesion receptors. (njit.edu)
  • Compared to tissue culture plastic or stiff gel substrates coated with laminin, on which astrocytes overgrow neurons in mixed cultures, laminin-coated soft gels encourage attachment and growth of neurons while suppressing astrocyte growth. (njit.edu)
  • Merging extracellular domains: fold prediction for laminin G-like and amino-terminal thrombospondin-like modules based on homology to pentraxins. (embl.de)
  • Using a new method for construction and database searches of sequence consensus strings, we have identified a new superfamily of protein modules comprising laminin G, thrombospondin N and the pentraxin families. (embl.de)
  • Decorin inhibits cell attachment to thrombospondin-1 by binding to a KKTR-dependent cell adhesive site present within the N-terminal domain of thrombospondin-1. (embl.de)
  • Skin decorin (DCN) is an antiadhesive dermatan sulfate-rich proteoglycan that interacts with thrombospondin-1 (TSP) and inhibits fibroblast adhesion to TSP [Winnemoller et al. (embl.de)
  • In short-term adhesion assays, the preferred substrate for HCE-T attachment is placental laminin. (nih.gov)
  • In this study, we demonstrate that the cross-linking of cell surface high-affinity IgE-R on mast cells derived from mouse bone marrow cultured for 3 wk in the presence of WEHI-3-conditioned media acts as a highly sensitive physiologic stimulus for this attachment and that receptor activation is also induced by calcium ionophore A23187. (aai.org)
  • Many of the effects of laminin are mediated through interactions with cell surface receptors. (wikipedia.org)
  • Of specific interest is the role that nanoscale cell-surface interactions play in the biocompatibility of neural implants. (frontiersin.org)
  • Furthermore, these interactions were aberrant in an adhesion-defective F9 cell line that results, at least in part, from abnormal oligosaccharide synthesis. (tmc.edu)
  • To find better therapies, patient-specific models that mimic the biology of tumour tissues and interactions between different cell types are developed. (ipfdd.de)
  • The CellSphere™ 3D Cell Culture Plate is a series of the cell culture plates with natural 3D environment that is scaffold-free and that maximizes cell-to-cell interactions. (biomaterialsusa.com)
  • A fucan prepared from Spatoglossum schröederi was tested as a possible inhibitor of cell-matrix interactions using wild-type Chinese hamster ovary cells (CHO-K1) and the mutant type deficient in xylosyltransferase (CHO-745). (embrapa.br)
  • In most cases, the cultivation surface has a negative charge and adhesion is believed to be mediated by protein bridges or ionic interactions (e.g., via Ca 2+ ). (capricorn-scientific.com)
  • The consecutive stages of cancer growth and dissemination are obligatorily perpetrated through specific interactions of the tumor cells with their microenvironment. (hindawi.com)
  • The consecutive steps of tumor growth, local invasion, intravasation, extravasation, and invasion of anatomically distant sites are obligatorily perpetrated through specific interactions of the tumor cells with their microenvironment. (hindawi.com)
  • Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)
  • On the ventral surface this rearward movement of the cytoskeleton contributes to forward motion of the cell relative to the substrate. (biologists.com)
  • As adherent cells grow, the substrate eventually becomes fully occupied. (capricorn-scientific.com)
  • In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)
  • Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
  • Dissociated embryonic rat cortices grown on flexible fibrin gels, a biomaterial with potential use as an implant material, display a similar mechano-dependent difference in cell population. (njit.edu)
  • Both RPSA and laminin belong to the heparin/heparan sulfate interactome. (wikipedia.org)
  • We have confirmed that both cell types synthesize and secrete heparan sulfate and chondroitin sulfate. (embrapa.br)
  • Hence, altering expression of CS and heparan sulfate (HS) on surface of tumor cells is a potential molecular strategy to prevent malignant transformation and tumor metastasis [ 2 ]. (biomedcentral.com)
  • We have investigated Aplysia hemolymph as a source of endogenous factors to promote regeneration of bag cell neurons. (nature.com)
  • Enhanced regeneration of dissociated bag cell neurons was observed in the presence of central nervous system (CNS) sheath cells or arterial cells 12 , or material remaining after such cells were killed 13 . (nature.com)
  • Culture of forebrain neurons was unusually simple and reliable, and some of these cells undergo early events of axonal-dendritic polarity. (biologists.com)
  • Astroglial cells derived from lateral and medial midbrain sectors differ in their abilities to support neuritic growth of midbrain neurons in cocultures. (embrapa.br)
  • Hence, the high-affinity IgE-R on the mast cell functions not only in exocytosis but also facilitates the process of mast cell adherence to laminin. (aai.org)
  • Using yeast and mammalian neuronal cells we find that Hsp90 and its co-chaperone Sti1 have the capacity to alter TDP-43 misfolding, inclusion formation, aggregation, and cellular toxicity. (bvsalud.org)
  • One of the primary mechanisms to modulate cellular invasivity is usually via differential adhesion to extracellular matrix (ECM). (biomedigs.org)
  • Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
  • The internal surfaces of cellulose acetate hollow fibres, of comparable internal diameter to small blood vessels, were coated with normal serum human IgG, heat-aggregated IgG (HAIgG), laminin or fibrinogen. (dundee.ac.uk)
  • Moreover, the C-domain of RPSA and heparin compete for binding to laminin, which shows that the highly acidic C-domain of RPSA mimicks heparin (and potentially heparan sulfates) for the binding to laminin. (wikipedia.org)
  • These data indicated that the increase in cell adhesions expected with increased cell-matrix interaction in differentiated F9 cells is not the consequence of increased surface GalTase expression and, more interestingly, that the two pools of GalTase are under differential regulation. (tmc.edu)
  • Regulation of adhesion of mouse bone marrow-derived mast cells to laminin. (aai.org)
  • Regulation of laminin-mediated signaling in other systems has also been described (20). (biomedigs.org)
  • GAGs also exert anti-cancer effects by altering structures of various tumor cells through sulfated modifications, including chondroitin sulfate (CS) and dermatan sulfate (DS). (biomedcentral.com)
  • However, it has widely been demonstrated that tumor cells negatively manage immune attack by activating several immune-suppressive mechanisms. (encyclopedia.pub)
  • It also acts as a cell surface receptor, in particular for laminin, and is involved in several pathogenic processes. (wikipedia.org)
  • ICM cells receive, from diverse sources, complex molecular and spatiotemporal signals that orchestrate the finely-tuned processes associated with embryogenesis. (bvsalud.org)
  • This simplified approach towards understanding the essence of the mechanisms, underlying the processes determining life and death of a cell has undoubtedly provided scientists with enormous amount of knowledge. (intechopen.com)
  • After the use of search strategies, 18 articles that describe processes of cell adhesion to bone grafts were selected. (bvsalud.org)
  • We have studied the synthesis and secretion of sulfated glycosaminoglycans (GAGs) by the two cell types under control conditions and ß-D-xyloside-stimulated conditions, that stimulate the ability to synthesize and release GAGs. (embrapa.br)
  • At the age of defect onset, GPR56 expression is restricted specifically to developing granule cells in the rostral cerebellum, suggesting that GPR56 regulates properties of these cells. (jneurosci.org)
  • cancer cell growth and spreading, it has also been proposed that GPR56 regulates cell adhesion, but this has remained untested in the nervous system. (jneurosci.org)
  • Variants of leukocyte adhesion deficiency have also been reported, including fully expressed but nonfunctional CD18 and an E selectin that is expressed but rapidly cleaved from the cell surface (only present in soluble form). (medscape.com)
  • Arrest on HAIgG was inhibited by excess soluble IgG but not by bovine serum albumin (BSA), demonstrating that the adhesion was IgG-specific and presumably mediated by FcγR on the PMN surface. (dundee.ac.uk)
  • This adhesion was similarly inhibited by excess soluble IgG, confirming the capability of FcγR to mediate cell arrest. (dundee.ac.uk)
  • The process of metastasis involves the spread of neoplastic cells to locoregional or distant body sites via lymphatic vessels and/or bloodstream. (hindawi.com)
  • This protein enhances cell motility, invasion and metastasis of cancer cells. (cancer-genetics.org)
  • It has been observed that the level of RPSA transcript is higher in colon carcinoma tissue and lung cancer cell lines than their normal counterparts. (wikipedia.org)
  • Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
  • After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • [12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
  • miRNAs are also an important cargo of the EVs that have been implicated in tissue morphogenesis and have gained special attention due to their ability to regulate protein expression through post-transcriptional modulation, thereby influencing cell phenotype. (bvsalud.org)
  • May play a role in cell fate determination and tissue morphogenesis. (joplink.net)
  • Accurate identification of these mechanisms of cell adhesion is essential for further advancement in tissue engineering, such as the production of biological bone substitutes that achieve a better clinical outcome. (bvsalud.org)
  • The use of nanotechnology plays an important role in tissue engineering because the properties that this technology adds to the material, such as the greater surface area and greater roughness, improve the physical-chemical properties which mimic those of the natural tissues and organs 31 . (bvsalud.org)
  • To this end, ELRs containing specific cell-adhesive (RGD) and/or osteoinductive (SNA15) moieties were covalently attached to Ti scaffolds. (ibecbarcelona.eu)
  • Specifically, these extracellular matrix (ECM) components critically modulate the tumor cell "motile phenotype" affecting their adhesive/migratory abilities which are directly correlated to the metastatic cascade [ 5 , 6 ]. (hindawi.com)
  • Some reports suggest that the design of the IOL is important, 10 12 13 others state that the adhesive properties of the lens material may be relevant, 6 7 10 as soft acrylic IOLs show greater lens epithelial cell (LEC) adhesion than do PMMA and silicone lenses. (arvojournals.org)
  • abstract = "An artificial material coated with a laminin-apatite composite layer on its surface would be useful as a percutaneous device with cell-adhesion properties and good biocompatibility. (elsevierpure.com)
  • abstract = "Human polymorphonuclear cells (PMN) were found to adhere to a novel model of blood vessel wall-associated IgG. (dundee.ac.uk)
  • NOTCH3-ADCs internalize into receptor and ligand cells impartial of signaling and induce cell dying in each cell sorts representing an atypical mechanism of ADC cytotoxicity. (kashbiotech.com)
  • EGFRvIII impacts both cell-intrinsic and non-cell autonomous aspects of GBM progression, including cell invasion, angiogenesis and modulation of the tumor microenvironment. (cancer-genetics.org)
  • Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines . (wikipedia.org)
  • This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • Audrey has experience with cultures and applications of primary and engineered immune cells as well as with high throughput 2D and 3D assays. (cellculturedish.com)
  • 1992]. Molecular mechanisms by which DCN interacts with TSP and inhibits cell adhesion to TSP are unknown. (embl.de)
  • Treatment of cells with staurosporin, which inhibits protein kinase C, also inhibited adherence and histamine release induced by calcium ionophore, but was not significantly active against either adherence or histamine release induced by Ag. (aai.org)
  • Xeno-free and serum-free cell culture products for supporting the culture of human stem cells and regular cells in 2D and 3D. (biomaterialsusa.com)
  • Aberrant NOTCH3 signaling and overexpression is oncogenic, related with most cancers stem cells and drug resistance, but therapeutic concentrating on stays elusive. (kashbiotech.com)
  • In this procedure it is possible to use stem cells obtained from different sources. (bvsalud.org)
  • Herein, a comprehensive hemocompatibility study after heparinized blood contact with seven polymer brushes prepared by surface-initiated atom transfer radical polymerization is reported. (ibecbarcelona.eu)
  • Compared with an unmodified glass surface, the grafting of polymer brushes minimizes the adhesion of platelets and leukocytes and prevents the thrombus formation. (ibecbarcelona.eu)
  • The objective of this study was to evaluate the anti-cancer effect of insect-derived polymer dung beetle glycosaminoglycan (GAG) after intraperitoneally injecting it to melanoma mice induced by B16F10 cells. (biomedcentral.com)
  • In addition, treatment with N-glycans derived from theses glycosaminoglycan increased activities of TIMP-2 in HMVEC cells pretreated with TNF-alpha and in melanoma cells, suggesting that they had anti-inflammatory and anticancer activities. (biomedcentral.com)
  • However, higher doses of statins may have a role to play in adjuvant therapy by inhibiting growth and invasion of melanoma cells. (biomedcentral.com)
  • Western blot analysis was used to partially characterize commercially available preparations of laminin isolated from the mouse EHS sarcoma and from human placenta. (nih.gov)
  • Free glycosaminoglycans (GAGs) and proteoglycan- (PG-) containing GAGs, key effectors of cell surface, pericellular and extracellular microenvironments, perform multiple functions in cancer by virtue of their coded structure and their ability to interact with both ligands and receptors that regulate cancer growth [ 1 - 4 ]. (hindawi.com)
  • GalTase-based adhesions are formed by recognition and binding to terminal N-acetylglucosamine (GlcNAc) residues on its glycoprotein counterpart on adjacent cell surfaces. (tmc.edu)
  • Glycosylation reactions of different glycosyltransferases can form complex sugar chains of secreted or cell-surface glycoprotein ( N -glycans and O -glycans) that can be added at asparagine residues ( N -linked) or at serine and threonine residues ( O -linked) [ 6 ]. (biomedcentral.com)
  • Mouse monoclonal to CD86.CD86 also known as B7-2,is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors.It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. (researchtoactionforum.org)
  • To determine molecular mechanism involved in the anti-cancer effect of dung beetle GAG, its origin N-glycan under 3KD Dalton was assayed for melanoma cell cytotoxicity. (biomedcentral.com)
  • This study was conducted to define the potential sequence(s) originating from the G domain of laminin with any of these functional activities. (rupress.org)
  • GalTase-specific adhesions are functional in a number of important biological events, including F9 embryonal carcinoma (EC) cell adhesions. (tmc.edu)