• Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human CCAAT/Enhancer Binding Protein Alpha (CEBPa) in Tissue homogenates, cell lysates and other biological fluids. (tissue-cell-culture.com)
  • CCAAT/enhancer-binding protein alpha is a protein encoded by the CEBPA gene in humans. (wikipedia.org)
  • CCAAT/enhancer-binding protein alpha is a transcription factor involved in the differentiation of certain blood cells. (wikipedia.org)
  • For details on the CCAAT structural motif in gene enhancers and on CCAAT/Enhancer Binding Proteins see the specific page. (wikipedia.org)
  • One category of mutations prevent CCAAT/enhancer-binding protein alpha DNA binding by altering its COOH-terminal basic leucine zipper domain. (wikipedia.org)
  • The other category of mutations disrupt the translation of the CCAAT/enhancer-binding protein alpha NH2 terminus. (wikipedia.org)
  • CEBPA mutations, which result in diminished CCAAT/enhancer-binding protein alpha activity, contribute to the transformation of myeloid antecedents. (wikipedia.org)
  • It has been shown that suppression of CEBPA expression and blocking of CCAAT/enhancer-binding protein alpha stops the differentiation of myeloid progenitors. (wikipedia.org)
  • For this reason, CCAAT/enhancer-binding protein alpha's role during granulocyte differentiation and CEBPA's role as a tumor suppressor gene is critically important in the prognosis of acute myeloid leukemia. (wikipedia.org)
  • CCAAT/enhancer-binding protein alpha, the transcription factor that is encoded by CEBPA, is very important in the differentiation of immature granulocytes. (wikipedia.org)
  • Results DIM, but not I3C, increased adipocyte differentiation through upregulation of peroxisome proliferator‐activated receptor γ and CCAAT/enhancer‐binding protein α. (researchgate.net)
  • We evaluated the characteristics of CCAAT/enhancer-binding protein α ( CEBPA ) mutations and the significance of a basic leucine zipper in-frame mutation (bZIP in-f ) of CEBPA in patients with acute myeloid leukemia with a normal karyotype. (e-crt.org)
  • CCAAT/enhancer-binding protein α ( CEBPA ) is a member of the family of basic leucine zipper (bZIP) transcription factors. (e-crt.org)
  • CCAAT enhancer binding protein alpha [S. (gsea-msigdb.org)
  • A recent report from the Children's Oncology Group reported that CCAAT enhancer-binding protein α (CEBPA) basic-region leucine zipper motif (bZip) domain mutations in patients with acute myeloid leukaemia are associated with favourable clinical outcomes, regardless of mono or bi-allelic status. (bjh.be)
  • Biallelic CCAAT enhancer-binding protein α ( CEBPA ) mutations are associated with favourable outcomes in acute myeloid leukaemia (AML). (bjh.be)
  • We evaluated concurrent gene mutations, clinical outcome, and gene expression signatures of CCAAT/enhancer binding protein alpha (CEBPA) double mutations (CEBPA(dm)) versus single mutations (CEBPA(sm)) in 1182 cytogenetically normal acute myeloid leukemia (AML) patients (16-60 years of age). (qxmd.com)
  • Additionally, TME concentrations ranging from 20 µg/mL to 500 µg/mL led to a decrease in the expression of adipocyte differentiation regulators, peroxisome proliferator-activated receptor γ, CCAAT element binding protein α, and sterol regulatory element binding transcription factor 1. (foodandnutritionresearch.net)
  • Several mechanisms have been postulated, including CCAAT/enhancer-binding protein-beta (C/EBP-β)-triggered macrophage-mediated muscle fiber regeneration after micro-injury, evidenced in a mouse model. (exeter.ac.uk)
  • The proximal region of the cNANOG promoter contains a positive transcriptional regulatory element (CCAAT/enhancer-binding protein ( CEBP )-binding site) in ESCs. (biomedcentral.com)
  • During the rest period of the day, they found a circadian protein called CCAAT enhancer binding protein alpha (CEBPA) causes a rapid increase in the production of PPARG. (bariatricnews.net)
  • Adipogenic marker genes, such as CCAAT/enhancer binding protein α ( Cebpa ), peroxisome proliferator-activated receptor γ ( Pparg ), adipocyte fatty acid binding protein ( Ap2 ), and fatty acid synthase ( Fas ), are regarded as the essential transcriptional regulators of preadipocyte differentiation and lipid storage in mature adipocytes. (endocrinology-journals.org)
  • CCAAT enhancer binding protein beta [Source. (gsea-msigdb.org)
  • CCAAT enhancer binding protein delta [Sourc. (gsea-msigdb.org)
  • There are two major categories which CEBPA mutations can be categorized into. (wikipedia.org)
  • In recent studies CEBPA mutations were found in between 7% and 15% of patients with acute myeloid leukemia. (wikipedia.org)
  • Many reports link CEBPA mutations with a favorable outcome in acute myeloid leukemia. (wikipedia.org)
  • Patients with CEBPA mutations have longer remission duration and survival time than those without the mutations. (wikipedia.org)
  • Therefore, the presence of CEBPA mutations are directly associated with a more favorable course for the progression of the disease. (wikipedia.org)
  • For example, in cases with CEBPA mutations, WHO 2022 includes biallelic (bi CEBPA ) and single mutations located in the basic leucine zipper (bZIP) region of the gene (smbZIP- CEBPA ) while ICC 2022 only includes in-frame bZIP CEBPA mutations. (cap.org)
  • The changes are made due to the favorable prognosis associated with CEBPA mutation is predominantly associated with in-frame bZIP CEBPA mutations based on recent studies. (cap.org)
  • Based on updated knowledge of CEBPA mutations, we conducted next-generation sequencing analyses in a previously established real-world cohort. (e-crt.org)
  • CEBPA mutations occur in 7.5%-11% of cases of de novo acute myeloid leukemia (AML) and predominantly in patients with a normal karyotype (NK-AML) with 15%-18% [ 4 - 6 ]. (e-crt.org)
  • Two major types of CEBPA mutations have been identified in AML, and patients can have double or single mutations. (e-crt.org)
  • CEBPA mutations affect either the N-terminus with nonsense mutations, leading to a dominant negative protein, or the C-terminus, resulting in decreased DNA binding of a leucine zipper region. (e-crt.org)
  • CEBPA mutations are detected in 7% to 15% of AML cases. (bjh.be)
  • Approximately one-third of CEBPA- mutated AML patients demonstrate a single involved allele, while two-thirds of patients have biallelic mutations. (bjh.be)
  • Thus far, studies, mainly in adult patients, supported that only biallelic CEBPA mutations result in improved event-free survival (EFS) and/or overall survival (OS) in AML patients. (bjh.be)
  • evaluated the clinical and biologic implications of CEBPA -basic leucine zipper ( CEBPA -bZip) mutations in children and young adults with newly diagnosed AML. (bjh.be)
  • CEBPA -bZip mutations were identified in 160 (5.4%) of 2,958 patients. (bjh.be)
  • Comprehensive NGS of patients with CEBPA mutations identified co-occurring CSF3R mutations in 13.1% of patients. (bjh.be)
  • CEBPA -bZip mutations are associated with favorable prognosis in de novo AML: a report from the Children's Oncology Group. (bjh.be)
  • Sierre J and Nomdedeu F. CEBPA bZip mutations: just a single shot. (bjh.be)
  • Prognostic impact, concurrent genetic mutations, and gene expression features of AML with CEBPA mutations in a cohort of 1182 cytogenetically normal AML patients: further evidence for CEBPA double mutant AML as a distinctive disease entity. (qxmd.com)
  • Outcome in CEBPA(sm) is dominated by concurrent NPM1 and/or FLT3 internal tandem duplication mutations. (qxmd.com)
  • This study examines the genetic events initiating leukemia and details the clinical progression of disease across multiple families harboring germ-line CEBPA mutations. (ox.ac.uk)
  • Germline CEBPA mutations clustered within the N-terminal and were highly penetrant, with AML presenting at a median age of 24.5 years (range, 1.75-46 years). (ox.ac.uk)
  • In all diagnostic tumors tested (n = 18), double CEBPA mutations (CEBPAdm) were detected, with acquired (somatic) mutations preferentially targeting the C-terminal. (ox.ac.uk)
  • Somatic CEBPA mutations were unstable throughout the disease course, with different mutations identified at recurrence. (ox.ac.uk)
  • Deep sequencing of diagnostic and relapse paired samples confirmed that relapse-associated CEBPA mutations were absent at diagnosis, suggesting recurrence was triggered by novel, independent clones. (ox.ac.uk)
  • The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and gene enhancers. (wikipedia.org)
  • Among 78 of a total of 395 patients (19.7%), 50 had bZIP in-f CEBPA , and 28 had non-bZIP in-f CEBPA . (e-crt.org)
  • In the multivariate analysis, patients with NPM1 mut , those with bZIP in-f CEBPA , and those who underwent allogeneic hematopoietic cell transplantation (allo-HCT) had favorable overall survival (OS), but FLT3 -ITD mut was a poor prognostic indicator. (e-crt.org)
  • In the CEBPA double-mutated group ( CEBPA dm ), bZIP in-f CEBPA was associated with superior outcomes in terms of OS (p=0.007) and RFS (p=0.007) compared with non-bZIP in-f CEBPA . (e-crt.org)
  • Of 50 patients with bZIP in-f CEBPA , 36 patients had at least one mutation. (e-crt.org)
  • Only bZIP in-f CEBPA was associated with favorable outcomes in patients with CEBPA dm . (e-crt.org)
  • thus, further studies with larger numbers of patients are required for clear conclusions of the significance of bZIP in-f CEBPA . (e-crt.org)
  • The carboxyterminal moiety of CEBPA has a basic-region leucine zipper motif (bZip). (bjh.be)
  • CEBPA -bZip mutation status was determined in 2,958 patients treated on consecutive Children's Oncology Group protocols (NCT00003790, NCT0007174, NCT00372593, NCT01379181). (bjh.be)
  • Of those 160 patients, 132 (82.5%) harboured a second CEBPA mutation ( CEBPA -double-mutated [ CEBPA -dm]) while the remaining 28 patients (17.5%) had a single CEBPA -bZip only mutation. (bjh.be)
  • Transcriptome analysis demonstrated similar expression profiles for patients with CEBPA -bZip and CEBPA -dm. (bjh.be)
  • In addition, CEBPA is essential for myeloid lineage commitment and therefore required both for normal mature granulocyte formation and for the development of abnormal acute myeloid leukemia. (wikipedia.org)
  • It has been shown that mutation of CEBPA has been linked to good outcome in both adult and pediatric acute myeloid leukemia patients. (wikipedia.org)
  • Mutation of the CEBPA gene has been shown to play a crucial role in leukemogenesis and prognosis in acute myeloid leukemia patients. (wikipedia.org)
  • For this reason it has been proposed that methylation of CEBPA could be a very useful biomarker in acute myeloid leukemia prognosis. (wikipedia.org)
  • Clinical data were collected from 10 CEBPA-mutated families, representing 24 members with acute myeloid leukemia (AML). (ox.ac.uk)
  • The protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. (wikipedia.org)
  • Targeting a DNA binding motif of the EVI1 protein by a pyrrole-imidazole polyamide. (rochester.edu)
  • Mechanistically, SEs recruited bromodomain containing 4 (BRD4) at the site of ZBTB16 , which then bound to RNA polymerase II-associated protein 2 (RPAP2) that transported RNA polymerase II (POL II) into the nucleus. (nature.com)
  • We further found that SET domain bifurcated 1 (Setdb1) was a SUMOylated protein and that SUMOylation promoted Setdb1 occupancy on the promoter locus of Pparg and Cebpa genes to suppress their expressions by H3K9me3. (deepdyve.com)
  • GA binding protein transcription factor. (gsea-msigdb.org)
  • WDR45 and WDR45B, but not their disease-related mutants, bind to the tether protein EPG5 and facilitate its targeting to late endosomes/lysosomes. (biomed.news)
  • TATA-box binding protein [Source:HGNC Symbo. (gsea-msigdb.org)
  • retinol binding protein 2 [Source:HGNC Symb. (gsea-msigdb.org)
  • E1A binding protein p300 [Source:HGNC Symbo. (gsea-msigdb.org)
  • C-terminal binding protein 1 [Source:HGNC S. (gsea-msigdb.org)
  • CREB binding protein [Source:HGNC Symbol;Ac. (gsea-msigdb.org)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human GA Binding Protein Transcription Factor Alpha (GABPa) in samples from serum, plasma, tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (therabio.org)
  • In adipocyte Senp2-de﫿ciency mice, accumulation of the SUMOylated Setdb1 suppressed the expression of Pparg and Cebpa genes as well as lipid metabolism-related target genes, which would decrease the ability of lipid storage in adipocytes. (deepdyve.com)
  • adqcKO Mechanistically, adipocyte Senp2 de﫿ciency caused the downregula- Senp2 mice exhibit an ectopic lipid accumulation and tion of Pparg and Cebpa as well as their downstream target genes insulin resistance related to lipid storage. (deepdyve.com)
  • Discussion : Taken together, the results of this study demonstrate that TME inhibits lipid accumulation and reduces the expression PPARG, CEBPA, and SREBF1, which regulate adipocyte differentiation in 3T3-L1 cells. (foodandnutritionresearch.net)
  • Our data reveal that familial CEBPA-mutated AML exhibits a unique model of disease progression, associated with favorable long-term outcomes. (ox.ac.uk)
  • CHAF1B has a pro-leukemia effect by binding chromatin at discrete sites and interfering with occupancy of transcription factors that promote myeloid differentiation, such as CEBPA. (telethonkids.org.au)
  • A recent study has found that higher levels of CEBPA methylation are directly proportionate with treatment response. (wikipedia.org)
  • The complete response rate increased proportionately with the level of CEBPA methylation. (wikipedia.org)
  • Dr. Daniel Tenen and his colleagues at Harvard Medical School had suspicions that active transcription might regulate levels of genomic DNA methylation, and dove in to the methylation dynamics of the well-known methylation-sensitive gene CEBPA, including the potential involvement of non-coding RNAs (ncRNAs) originating from that locus. (epigenie.com)
  • The novel RNA ecCEBPA, arising from the CEBPA gene locus, is critical in regulating the local DNA methylation profile. (epigenie.com)
  • ecCEBPA binds to DNMT1 and prevents CEBPA gene locus methylation. (epigenie.com)
  • The activity of these elements is controlled by transcription factors (TFs) which bind to DNA wrapped into chromatin, leading to the modification of the chromatin landscape, and the assembly of the transcription machinery. (amegroups.org)
  • Unlike sequence-specific transcription factors, epigenetic regulators do not necessarily bind DNA at consensus sequences, but still achieve reproducible target binding in a manner that is cell and maturation-type specific. (frontiersin.org)
  • Gene transcription is mainly regulated by transcription factors (TFs) that bind to specific DNA sequences (called motifs) located in the promoter regions of genes [ 1 ]. (biomedcentral.com)
  • Some have been shown to bind epigenetic regulators, and thus constitute attractive candidates to mediate epigenetic target specificity. (frontiersin.org)
  • We extend the network to include the downstream regulators FOG1 and CEBPA, and extract the nature of the corresponding regulatory interactions, excitatory or suppressing, between this pair and the triad by fitting to experimental gene expression time series. (lu.se)
  • CEBPA is a transcription factor coded by an intronless gene located on chromosome 19. (bjh.be)
  • What is not in the paper is that we can design CRISPR-like RNA guide oligos connected to RNA promoter sequences, which can induce demethylation of CEBPA…We are now testing this method with other gene loci. (epigenie.com)
  • Sequence-dependent specificity was confirmed by modifying 2 to 3 nucleotide residues in the seed binding region of the lead sequences, which in each case significantly reduced induction of target mRNA compared to the parental saRNA sequences. (aacrjournals.org)
  • Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs) (By similarity). (cansar.ai)
  • Without SEs located on osteogenic genes, BRD4 is not able to bind to osteogenic identity genes due to its closed structure before osteogenesis. (nature.com)
  • During osteogenesis, histones on osteogenic identity genes are acetylated, and OB-gain SEs appear, enabling the binding of BRD4 to the osteogenic identity gene ZBTB16. (nature.com)
  • Using network dynamical parameters determined by the data, we deduce from increasing the self-interaction bindings in silico among the triad genes that GATA2 and PU.1 exhibit non-linear behavior with one unstable and one stable state. (lu.se)
  • It is transcriptional activator that recognizes and binds to the sequence 5'-RTTAYGTAAY-3' found in the promoter of genes such as albumin, CYP2A4 and CYP2A5. (avivasysbio.com)
  • Recently it has been shown that epigenetic modification of the distal promoter region of CEBPA has resulted in downregulation of CEBPA expression in pancreatic cancer cells, lung cancer, and head and neck squamous cell carcinoma. (wikipedia.org)
  • EVI1 Interferes with Myeloid Maturation via Transcriptional Repression of Cebpa, via Binding to Two Far Downstream Regulatory Elements. (rochester.edu)
  • Next, CEBPA mutational status was correlated with disease characteristics and clinical outcomes. (bjh.be)
  • Recent extensive binding and gene expression experiments with some focus on the triad GATA1, GATA2 and PU.1 indicate that GATA2 may be more involved in this lineage decision than previously anticipated. (lu.se)
  • Near-study-wide associations were also noted for TGF-β3 (P = 3.4*10(-5) , q = 0.12) and CEBPD expression (P = 9.7*10(-5) , q = 0.18) but not for CEBPA expression. (exeter.ac.uk)
  • We have developed MTL-CEBPA, an saRNA-based therapeutic designed to increase the expression of the CEBPA transcription factor that acts as both a tumor suppressor and a key modulator of the tumor microenvironment. (aacrjournals.org)
  • Direct binding of ZFP143 to the proximal region of the NANOG promoter regulates NANOG expression by modulating OCT4 binding [ 24 ]. (biomedcentral.com)
  • Unsupervised and supervised GEP analyses showed that CEBPA(dm) AML (n = 42), but not CEBPA(sm) AML (n = 18), expressed a unique gene signature. (qxmd.com)
  • After the binding of the RPAP2-Pol II complex to BRD4 on SEs, RPAP2 dephosphorylates Ser5 at the Pol II CTD to terminate the transcriptional pause, and BRD4 phosphorylates Ser2 at the Pol II CTD to initiate transcriptional elongation, which synergistically drives efficient transcription of ZBTB16, ensuring proper osteogenesis. (nature.com)
  • We designed saRNA to target the IL-23A, IL-36g, OX40L, and UCP2 transcripts using our proprietary algorithm that bind to long noncoding RNA at position +2000 to -2000 nucleotides relative to the transcriptional start site. (aacrjournals.org)
  • Remarkably, the clinical and laboratory features of the both CEBPA cohorts appeared to be very similar. (bjh.be)
  • In preclinical tumor models MTL-CEBPA has single-agent antitumor activity and very significantly improves the activity of both sorafenib and checkpoint inhibitors through impacting the immunosuppressive tumor microenvironment. (aacrjournals.org)
  • JASPAR is an open-access database of curated, non-redundant transcription factor (TF) binding profiles stored as position frequency matrices (PFMs) and TF flexible models (TFFMs) for TFs across multiple species in six taxonomic groups. (genereg.net)
  • Upon binding of their respective ligands and activation, intracellular signalling cascades, often involving phophorylation are induced which eventually terminate at inducible TFs and regulate their activity. (amegroups.org)
  • NCALD (Neurocalcin Delta) is a member of the Neuronal Calcium Sensor family of calcium binding proteins. (biomedcentral.com)
  • Exceptions are acute promyelocytic leukemia (APL) and core binding factor AML (i.e. (cap.org)
  • CEBPA has been shown to interact with Cyclin-dependent kinase 2 and Cyclin-dependent kinase 4. (wikipedia.org)
  • Tenen admits his surprise "that no one had ever asked whether DNMT1 bound RNA with high affinity and function, since it seems likely that anything that binds DNA will also bind RNA, and others have previously shown DNMT2 binds to RNA. (epigenie.com)
  • Based on these findings, we propose that CEBPA(dm) should be clearly defined from CEBPA(sm) AML and considered as a separate entity in the classification of AML. (qxmd.com)