• Carboxypeptidase A3 (mast cell carboxypeptidase A), also known as CPA3, is an enzyme which in humans is encoded by the CPA3 gene. (wikipedia.org)
  • The "CPA3" gene expression has only been detected in mast cells and mast-cell-like lines, and CPA3 is located in secretory granules. (wikipedia.org)
  • A gene nomenclature committee renamed mast cell carboxypeptidase A as CPA3, and the original CPA3 reported by Huang et al. (wikipedia.org)
  • Cloning and characterization of the novel gene for mast cell carboxypeptidase A". The Journal of Clinical Investigation. (wikipedia.org)
  • We and other groups discovered the human IL-17F gene from a human EST sequence, a genomic DNA clone, and T-cell cDNA sequences in 2001 [ 3 , 8 , 9 ]. (hindawi.com)
  • The following Cd82 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
  • The objective of this study was to identify additional cooperative gene(s) associated with BCL2 and MYC or MYC and CCND1 in human B-cell lymphomagenesis. (haematologica.org)
  • The FA2 gene of Chlamydomonas encodes a NIMA family kinase with roles in cell cycle progression and microtubule severing during deflagellation. (bio.net)
  • Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. (nih.gov)
  • buy unreal engine game development blueprints discover all the secrets of unreal engine and create seven fully functional games with the help of step gene between onset and value. (scoutconnection.com)
  • T. Napso, N. Azzam, A. Lerner and F. Fares, " N-myc Downstream Regulated Gene 1 Increases Differentiation Factors Level in Human Prostate Cancer Cells without Affecting Cell Proliferation and Cell Cycle Profiles," Journal of Cancer Therapy , Vol. 4 No. 2, 2013, pp. 568-574. (scirp.org)
  • A Novel Gene Which Is Up-Regulated during Colon Epithelial Cell Differentiation and Down-Regulated in Colorectal Neoplasms," Laboratory Investigation: A Journal of Technical Methods and Pathology, Vol. 77, No. 1, 1997, pp. 85-92. (scirp.org)
  • Differential display analysis of gene expression revealed the overexpression of translation elongation factor-1 delta sub-unit in BALB/c-3T3 cells transformed with cadmium chloride compared with the non- transformed control cells. (cdc.gov)
  • 1. McKinnon D (1989) Isolation of a cDNA clone coding for a putative second potassium channel indicates the existence of a gene family. (aspetjournals.org)
  • The murine CD2 gene was assigned to chromosome 3 by Southern blot analysis of mouse-hamster somatic cell hybrids. (ox.ac.uk)
  • First, a full-length functional ZIKV cDNA clone was engineered as a bacterial artificial chromosome, with each reporter gene under the cap-independent translational control of a cardiovirus-derived internal ribosome entry site inserted downstream of the single open reading frame of the viral genome. (mdpi.com)
  • The human RON gene was originally cloned from keratinocytes ( 1 ). (spandidos-publications.com)
  • In contrast to the above model, we find that an enhancer trap inserted near the Dpp target gene, Daughters against Dpp ( Dad ), is expressed in additional somatic cells within the germarium,suggesting that Dpp protein may be distributed throughout the anterior germarium. (biologists.com)
  • Despite multiple advances in cell engineering, the rapid creation of robust and multi-gene cell lines remains of reach - until now. (genelink.com)
  • These cells were selected after infection with HTLV-1 envelope pseudotyped viral particles expressing a drug resistance gene. (wustl.edu)
  • The human glucosamine-6-phosphate deaminase gene: cDNA cloning and expression, genomic organization and chromosomal localization. (nih.gov)
  • Production of the fusion polypeptide involves the introduction of the hybrid gene into a biological host cell system, for example yeast cells, which permits the expression and accumulation of the fusion polypeptide. (justia.com)
  • According to AceView, this gene is expressed at high level , 2.6 times the average gene in this release, at all stages of development [Kohara cDNAs]. (nih.gov)
  • The sequence of this gene is defined by 16 cDNA clones and 18 elements defined by RNA-seq, some from mixed (seen 7 times), embryo (5), l2 (once). (nih.gov)
  • This diagram shows in true scale the gene on the genome, the mRNAs and the cDNA clones. (nih.gov)
  • In combination with transgenic organisms, such enzyme-actuated antisense tools will enable gene silencing in specific cell types, including tissues that are not amenable to optical targeting. (cdc.gov)
  • 11 , 12 Light-dependent gene silencing can be achieved through whole-organism irradiation or the targeted illumination of specific cell populations. (cdc.gov)
  • Genes known to be highly expressed in β-cells were represented at a high frequency, namely insulin (15 of 80 clones), islet amyloid polypeptide (8 of 80 clones), proinsulin convertase 1 (6 of 80 clones), and neuropeptide Y (2 of 80 clones). (diabetesjournals.org)
  • Results Primary B cells exogenously expressing Bcl2 , Myc and Ccnd1 showed factor-independent growth ability, enhanced colony-forming capability and aggressive oncogenicity, unlike the cases observed with the expression of any combination of only two of the genes. (haematologica.org)
  • We identified CCND3 and NRAS as cooperative genes with Bcl2 and Myc through the functional screening. (haematologica.org)
  • The study of functional synergism among cancer-causing genes is thus becoming one of the central issues in cancer research. (haematologica.org)
  • These genes are transcriptionally deregulated as the partner genes of IgH translocation, and are thus thought to perform crucial roles in human B-cell lymphomagenesis. (haematologica.org)
  • 2 A synergistic effect resulting from a combination of two genes from BCL2 , MYC and CCND1 has been implicated in human B-cell lymphomagenesis. (haematologica.org)
  • 7 Importantly, it is believed that other hitherto unknown genes also play important roles in lymphomagenesis in addition to the synergistic effects of the aforementioned two oncogenes since human B-cell lymphomas often show a variety of genes subject to alterations and/or deregulated expression. (haematologica.org)
  • The approach was applied to five different hybridomas producing human monoclonal antibodies and variable regions for both bold gamma and mu heavy chain and kappa and lambda light chain genes were successfully cloned. (lu.se)
  • K. Kokame, H. Kato and T. Miyata, "Homocysteine-Respondent Genes in Vascular Endothelial Cells Identified by Differential Display Analysis. (scirp.org)
  • Significantly differentially expressed (SDE) immunological genes and transcription factor (TF) were selected for functional pathways and transcriptional signaling identification. (frontiersin.org)
  • The technology utilizes fluorogenic oligonucleotide signaling probes and flow cytometry to detect and isolate individual living cells expressing one or more genes. (genelink.com)
  • Originating at The Rockefeller University, Chromovert Technology is a cell engineering tool for rapid production of stable cell lines expressing one or more genes. (genelink.com)
  • After 10 weeks, exercised and non-exercised males were sampled to determine plasma 11-ketotestosterone levels, testicular mRNA expression levels of genes involved in steroidogenesis and gametogenesis by qPCR, as well as the relative abundance of germ cells representing the different spermatogenic stages by histological examination. (biomedcentral.com)
  • Chimeric Zika viruses containing structural protein genes of insect-specific flaviviruses cannot replicate in vertebrate cells due to entry and post-translational restrictions. (cdc.gov)
  • An analysis of 555 randomly chosen clones in the library showed that 80 were derived from abundant mRNAs and were accounted for by 29 distinct sequences. (diabetesjournals.org)
  • Many of the novel cDNA sequences that were highly represented in the library showed a relative specificity to β-cells compared with other tissues, including glucagonoma, liver, kidney, brain, 3T3 fibroblasts, and AtT20 corticotrophs, and warrant further investigation. (diabetesjournals.org)
  • These sequences represent the protein coding region of the Cd82 cDNA ORF which is encoded by the open reading frame (ORF) sequence. (genscript.com)
  • ORF sequences can be delivered in our standard vector, pcDNA3.1 + /C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. (genscript.com)
  • Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (nih.gov)
  • CHROMOVERT® PROCESS a) cDNAs are subcloned for expression of mRNAs comprising 3' untranslated plasmid-encoded Chromo-Tag™ sequences for detection using fluorogenic oligonucleotide signaling probes. (genelink.com)
  • Click on any transcript to open the specific mRNA page, to see the exact cDNA clone support and eventual SNPs and to get details on tissues, sequences, mRNA and protein annotations. (nih.gov)
  • The enzyme now called CPA3 was originally named mast cell carboxypeptidase A, and another protein was initially called CPA3. (wikipedia.org)
  • To define the localization of WDFY2 in the endocytic pathway, we transiently transfected hTERT-RPE1 cells with green flourescent protein (GFP)-WDFY2 and performed structured illumination microscopy (SIM) together with APPL1 and EEA1 visualized with antibodies (Fig. 1a ). (nature.com)
  • Protein phosphorylation regulates actomyosin-driven vesicle movement in cell extracts isolated from the green algae, Chara corallina. (bio.net)
  • cDNA cloning (GenBank Accession Number AF304351), expression of the encoded protein, and functional analysis of cDNA transfected NIH3T3 cells indicated that translation elongation factor-1 delta is a proto-oncogene. (cdc.gov)
  • The cDNA codes for a 427-amino acid protein, with two putative transmembrane domains and an H5 region. (xenbase.org)
  • These cDNAs all code for the same human protein that is a homologue of the murine MIP-1 alpha. (rndsystems.com)
  • The earliest biochemical evidence on the existence of cell-free soluble LH receptor was the purification of an hCG-binding protein, relative molecular mass of 65K (M r , 65K) from porcine follicular fluid and was based on gel filtration followed by affinity chromatography [ 9 ]. (biomedcentral.com)
  • In consequence, protein turnover of WT-TRPM4 and TRPM4 variants overexpressed in HEK293 cells was analyzed using cycloheximide, an inhibitor of protein biosynthesis. (frontiersin.org)
  • Maturation occurs in the cell membrane resulting in a 180-kDa heterodimeric protein composed of a 40-kDa α-chain and a 150-kDa transmembrane β-chain with intrinsic tyrosine kinase activity ( 5 , 6 ). (spandidos-publications.com)
  • RON is activated in response to macrophage-stimulating protein (MSP), and then induces an invasive program ( 8 ) consisting of cell proliferation, migration, and invasion, all of which are important at multiple points during tumorigenesis. (spandidos-publications.com)
  • Moreover, in females doubly mutant for bam and the ubiquitin protein ligase Smurf , the number of germ cells responsive to Dpp is greatly increased relative to the number observed in either single mutant. (biologists.com)
  • As part of a directed effort to discover novel G protein-coupled receptors through homology searching of expressed sequence tag databases, we identified a partial clone (GPCR97) that had significant homology to biogenic amine receptors. (aspetjournals.org)
  • The GPCR97 clone was used to probe a human thalamus library, which resulted in the isolation of a full-length clone encoding a putative G protein-coupled receptor. (aspetjournals.org)
  • The cDNA encodes a protein consisting of 386 amino acid residues with seven putative transmembrane domains and a deduced molecular weight of 40,956. (drugbank.com)
  • We found that Homer3, the predominant isoform in Purkinje cells, is phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) both in vitro and in vivo . (jneurosci.org)
  • In this study, we revealed that Homer3 was phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) in Purkinje cells, and the phosphorylation reduces the affinity for Homer target molecules, resulting in change of the solubility. (jneurosci.org)
  • Mutations that abrogated the hbz mRNA or disrupted a stem-loop in hbz mRNA, or mutations that eliminated or truncated the HBZ protein were introduced in a functional molecular clone of HTLV-1. (wustl.edu)
  • Repression of Pumilio Protein Expression by Rbfox1 Promotes Germ Cell Differentiation. (tcd.ie)
  • The tests used for protein C testing can include enzyme-linked immunosorbent assay (ELISA), chromogenic assays, and the activated partial thromboplastin time (aPTT)-based functional protein C assay. (medscape.com)
  • An ELISA measures protein C immunological levels with very high sensitivity but cannot detect functional defects. (medscape.com)
  • Chromogenic assays can detect low levels of protein C with high sensitivity and can detect most functional defects. (medscape.com)
  • The aPTT-based functional protein C assay may yield misleadingly low protein C levels in the presence of (1) a factor V Leiden mutation and some other causes of activated protein C resistance, (2) elevated plasma factor VIII levels, or (3) hyperlipidemia. (medscape.com)
  • For the functional screening, Bcl2 - and Myc- expressing primary B cells were infected with a retroviral cDNA library. (haematologica.org)
  • In an effort to identify a cellular receptor or attachment factor for HTLV-1, we carried out a retroviral cDNA library screen, in which cDNA from permissive HeLa S3 cells was introduced into poorly susceptible NIH 3T3 cells. (wustl.edu)
  • 3. Stuhmer W, Ruppersberg JP, Schroter KH, Sakmann B, Stocker M, Giese KP, Perschke A, Baumann A, and Pongs O (1989) Molecular basis of functional diversity of voltage-gated potassium channels in mammalian brain. (aspetjournals.org)
  • 1994), Molecular cloning and expression of a human hea. (xenbase.org)
  • Molecular cloning and expression of a human heart inward rectifier potassium channel. (xenbase.org)
  • The murine homologue of the T lymphocyte CD2 antigen: molecular cloning, chromosome assignment and cell surface expression. (ox.ac.uk)
  • Despite recent findings regarding the functional implications of TRPM4 in cardiac diseases, the molecular and cellular mechanisms leading to altered conduction are poorly understood. (frontiersin.org)
  • Kong AN, Yang L, Ma M, Tao D, Bjornsson TD: Molecular cloning of the alcohol/hydroxysteroid form (hSTa) of sulfotransferase from human liver. (t3db.ca)
  • Together, these findings suggested that Homer3 in Purkinje cells might function as a reversible coupler regulated by CaMKII phosphorylation and that the phosphorylation is capable of regulating the postsynaptic molecular architecture in response to synaptic activity. (jneurosci.org)
  • These results suggest that the phosphorylation may regulate postsynaptic molecular architecture and Ca 2+ signaling of Purkinje cells in response to synaptic activity. (jneurosci.org)
  • 1990. Cell replication and unscheduled DNA synthesis (UDS) activity of low molecular weight chlorinated paraffins in the rat liver in vivo. (cdc.gov)
  • Of these, 17 were identical or homologous to known mammalian cDNAs or expressed sequence tags. (diabetesjournals.org)
  • Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. (genscript.com)
  • Mammalian cell lines, especially those produced using immortalized lines like human embryonic kidney 293 (HEK 293) and Chinese hamster ovary (CHO) cells, are widely used in biological research, including in drug discovery and for biologics production. (genelink.com)
  • Molecularly cloned mammalian glucosamine-6-phosphate deaminase localizes to transporting epithelium and lacks oscillin activity. (nih.gov)
  • Cloning, expression, and differentiation-dependent regulation of SMAF1 in adipogenesis. (nih.gov)
  • Even though its sequence along with its expressional pattern in cancer cell lines are evident, the functional aspects concerning cell proliferation, viability, differentiation and cell cycle regulation of NDRG1 remains vague. (scirp.org)
  • These results revealed that NDRG1 is functional in prostate cancer cells and able to induce expression of differentiation factors through p53 independent pathway. (scirp.org)
  • Ly6C high MC presented downregulated co-stimulatory receptors (CD2, GITR, and TIM1) which direct immune cell proliferation, and upregulated co-stimulatory ligands (LIGHT and SEMA4A) which trigger antigen priming and differentiation. (frontiersin.org)
  • Finally, we established 3 groups of transcriptional models to describe Ly6C high to Ly6C low MC subset differentiation, immune checkpoint regulation, Ly6C high MC to MΦ subset differentiation and Ly6C low MC to lymphocyte functional adaptation. (frontiersin.org)
  • Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). (bvsalud.org)
  • Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues. (bvsalud.org)
  • Since the Notch signaling pathway molecules play an important role in differentiation of epithelial cells, it is important to identify the presence of notch signaling molecules in SHED during the process of cell differentiation. (bvsalud.org)
  • The Notch signaling pathway provides important intercellular signaling mechanisms essential for cell fate specification and it regulates differentiation and proliferation of stem or progenitor cells by para-inducing effects 3-4 . (bvsalud.org)
  • Notch signaling pathway is also involved in the regulation of epithelial cell differentiation in various tissues 5-6 . (bvsalud.org)
  • These data suggest that there may be subtypes of the PGE1 receptor in this clone: a high-affinity receptor mediating cyclic AMP formation, and a low-affinity receptor mediating IP accumulation, cyclic GMP formation, and intracellular calcium mobilization. (shengsci.com)
  • The human T lymphocyte antigen CD2 (T11, sheep erythrocyte receptor) is expressed on all peripheral T cells and all but the most immature thymocytes. (ox.ac.uk)
  • Experiments with monoclonal antibodies against CD2 suggest that CD2 is the cell surface receptor for a natural ligand involved in T cell proliferation. (ox.ac.uk)
  • The functional receptor for MIP-1 alpha has been identified as CCR1 and CCR5. (rndsystems.com)
  • The hormonal activation occurs through specific ligand-receptor interactions on the surface of the target cells. (biomedcentral.com)
  • A different experimental approach by West and Cooke [ 10 ] demonstrated the secretion of M r 80-90K hCG-LH receptor complex into the culture media from ligand-induced rat and mouse leydig cells. (biomedcentral.com)
  • 11 ] first reported the production and secretion of soluble LH receptor following transient transfection of a naturally truncated variant of the receptor in COS cells. (biomedcentral.com)
  • Additionally, the truncated Lhcgr, misrouted the full-length Lhcgr in ER, thereby decreasing the cell surface expression of the mature functional receptor. (biomedcentral.com)
  • instead, a specific intra-cellular role of truncated receptor in regulating the cell surface expression of a full-length ligand-binding Lhcgr has been proposed [ 15 ]. (biomedcentral.com)
  • Transient receptor potential melastatin member 4 (TRPM4), a non-selective cation channel, mediates cell membrane depolarization in immune response, insulin secretion, neurological disorders, and cancer. (frontiersin.org)
  • Recepteur d'origine nantais (RON) is a receptor tyrosine kinase (RTK) normally expressed at low levels in epithelial cells. (spandidos-publications.com)
  • Neurotensin receptor 3 gp85/sortilin NTSR3 NTSR3 functions as a modulator of neurotensinergic signaling when it is co-expressed with another receptor of NTS and as a functional receptor involved in the migration when expressed alone. (cancer8.info)
  • 2002 In the human microglial cell line C13NJ NTSR3 is the only known endogenous NTS receptor. (cancer8.info)
  • 2003 The NTS/NTSR3 complex has been shown to phosphorylate both Erk1/2 and Akt kinases in a murine microglial cell line (Dicou 2008 NEUROTENSIN/NEUROTENSIN RECEPTOR COMPLEX AND Malignancy BIOLOGY Few years after its discovery high-level expression of NTS was found in the plasma of pancreatic tumor patients (Gutniak et al. (cancer8.info)
  • 2012 The development promoting actions of NTS continues to be seen in pancreatic tumor cell lines both and and receptor synthesis a couple of hours after agonist removal (Souaze and Forgez 2006 Rules et al. (cancer8.info)
  • The availability of the human H 3 receptor cDNA should greatly aid in the development of chemical and biological reagents, allowing a greater appreciation of the role of histamine in brain function. (aspetjournals.org)
  • Cloning and expression of a cDNA for the human prostacyclin receptor. (drugbank.com)
  • A functional cDNA for the human prostacyclin receptor was isolated from a cDNA library of CMK cells, a human megakaryocytic leukaemia cell line. (drugbank.com)
  • Iloprost in a concentration-dependent manner increased the cAMP level and generated inositol trisphosphate in these cells, indicating that this human receptor can couple to multiple signal transduction pathways. (drugbank.com)
  • Ly6C high MC exhibited activated neutrophil degranulation, lysosome, cytokine production/receptor interaction and myeloid cell activation pathways, and Ly6C low MC presented features of lymphocyte immunity pathways in both mice. (frontiersin.org)
  • Furthermore, both robust phosphorylation of Homer3 and its dissociation from metabotropic glutamate receptor 1α (mGluR1α) were triggered by depolarization in primary cultured Purkinje cells, and these events were inhibited by CaMKII inhibitor. (jneurosci.org)
  • The functional high affinity IL-12 receptor is composed of at least IL12rb1 and IL12rb2. (lu.se)
  • BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
  • Cloning and characterization of an active fragment of luciferase from a luminescent marine alga, Pyrocystis lunula. (bio.net)
  • Cloning and functional characterization of GNPI2, a novel human homolog of glucosamine-6-phosphate isomerase/oscillin. (nih.gov)
  • Continued Need for Comprehensive Genetic and Phenotypic Characterization of Viruses: Benefits of Complementing Sequence Analyses with Functional Determinations. (cdc.gov)
  • Unpredictably, cell cycle progression, cell proliferation and DNA synthesis were unaffected fol lowing NDRG1 expression. (scirp.org)
  • However, the pathway downstream NDRG1, involving p21 and c8/18, regulating cell cycle progression and DNA synthesis is unfunctional. (scirp.org)
  • Reverse-transcriptase enzyme was used to convert extracted RNA into cDNA using reverse transcriptase cDNA synthesis kit (MMLV RT 1st-Strand cDNA synthesis kit, Korea) following the protocol provided by the manufacturer. (bvsalud.org)
  • Effects of chloroplast DNA content on the cell proliferation and aging in Chlamydomonas reinhardtii. (bio.net)
  • MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo . (rndsystems.com)
  • 2) The presence of LYSET is critical for cancer cell proliferation in nutrient-deprived environments in which extracellular proteins must be catabolized. (stanford.edu)
  • The RON cDNA encodes 1,400 amino acids, which are synthesized first as a single-chain precursor (pro-RON) ( 1 ). (spandidos-publications.com)
  • The sequence homology with KATP-1 suggests that BIR1 is a subunit of a brain and beta-cell KATP channel. (ox.ac.uk)
  • The murine cDNA sequence encoded a predicted mature polypeptide of 322 amino acids that showed 54% identity with the predicted human sequence. (ox.ac.uk)
  • A rabbit antiserum raised against purified human CD2, precipitated from surface-labeled mouse thymocytes a glycoprotein of Mr 55,000-66,000 which decreased to Mr 35,000 on digestion with endo-beta-acetylglucosaminidase F. These sizes are consistent with those predicted for the murine CD2 antigen from the cDNA sequence. (ox.ac.uk)
  • Despite a high level of sequence identity and similarity there is essentially no functional overlap between these carriers. (elsevierpure.com)
  • Proteins supported by a single continuous cDNA sequence lead to underlining the name/ending of the variant. (nih.gov)
  • The methods used for RT-PCR, sequencing, cDNA cloning, rapid amplification of cDNA ends (RACE), and sequence analysis were previously described ( 2 , 3 ). (cdc.gov)
  • Functional expression in Xenopus oocytes showed that HH- IRK1 is a K+ channel with strong inward rectification, blocked by extracellular Ba2+ and Cs+, and with a single-channel conductance of 30 pS. (xenbase.org)
  • Cloning and functional expression of the cDNA encoding an inwardly-rectifying potassium channel expressed in pancreatic beta-cells and in the brain. (ox.ac.uk)
  • A polymerase chain reaction-based subtractive hybridization procedure was applied to cDNAs prepared from mouse insulinoma (βTC3) and glucagonoma (αTC2) cell lines to construct a library of cDNAs that are highly expressed in pancreatic β-cells. (diabetesjournals.org)
  • When combined with functional or immunological screening procedures, the approach will be useful for the isolation of β-cell-specific molecules for immunological and genetic investigations of β-cell function and pathology. (diabetesjournals.org)
  • This methodology facilitates the isolation and recovery of the fusion peptide from the host cells by exploiting the physico-chemical properties of the fusion partner. (justia.com)
  • RNA Isolation and cDNA Microarrays. (lu.se)
  • Inhibition of cytotoxic effect of FASL on Jurkat cells in the presence of recombinant human FAS. (biolegend.com)
  • FAS was initially purified and cloned from SKW6.4 cells and a cDNA library of human T cell lymphoma KT-3 cells. (biolegend.com)
  • In addition, FAS/FASL interaction increases the production of chemokines in NK-T cells, and FASL acts as a survival factor for human CD34 + cells and increases their colony formation. (biolegend.com)
  • Human FAS, amino acids (Arg17-Asn173) (Accession# NM_000043), was expressed in 293E cells. (biolegend.com)
  • Human peripheral blood mononuclear cells (PBMC) were stained with the PE-Cy™7 Mouse Anti-Human CD194 antibody in conjunction with either a FITC Mouse Anti-Human CD4 antibody (Cat. (bdbiosciences.com)
  • Background A synergistic effect resulting from a combination of BCL2 and MYC or MYC and CCND1 has been implicated in human B-cell lymphomas. (haematologica.org)
  • Genetic alterations of BCL2 (B-cell CLL/lymphoma 2), MYC [v-myc myelocytomatosis viral oncogene homolog (avian)] and CCND1 (cyclin D1) are the most frequently found alterations in human B-cell lymphomas. (haematologica.org)
  • Importantly, human B-cell lymphoma cases with concurrent multiple translocations including BCL2 , MYC and CCND1 have been reported. (haematologica.org)
  • The aim of the present study was to elucidate the functional role of NDRG1 in human prostate cancer. (scirp.org)
  • Furthermore, human cDNA of NDRG1 from normal placenta was cloned into a eukaryotic expression vector and transfected into the three cancer cell lines. (scirp.org)
  • These results demonstrate that the cell transformation and tumorigenesis induced by the human carcinogen - cadmium , were due to the overexpression of translation elongation factor-1 delta - a novel cadmium -responsive proto-oncogene. (cdc.gov)
  • We have isolated a cDNA encoding an inwardly rectifying K+ channel (HH- IRK1 ) from human heart . (xenbase.org)
  • An alternative approach to the identification of the murine homologue was to use cross-species DNA hybridization, employing human CD2 cDNA as a probe. (ox.ac.uk)
  • Functional expression of inward rectifier potassium channels in cultured human pulmonary smooth muscle cells: evidence for a major role of Kir2.4 subunits. (genscript.com)
  • Functional expression of Kir2.x in human aortic endothelial cells: the dominant role of Kir2.2. (genscript.com)
  • Human MIP-1 alpha refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. (rndsystems.com)
  • We demonstrate that recombinant LHCGR proteins which include the hormone-binding domain are secreted from transfected cells and that natural LHCGR is also secreted from human placental explants. (biomedcentral.com)
  • Comer KA, Falany JL, Falany CN: Cloning and expression of human liver dehydroepiandrosterone sulphotransferase. (t3db.ca)
  • Forbes KJ, Hagen M, Glatt H, Hume R, Coughtrie MW: Human fetal adrenal hydroxysteroid sulphotransferase: cDNA cloning, stable expression in V79 cells and functional characterisation of the expressed enzyme. (t3db.ca)
  • 1996 Using CHO cells transfected with the cloned rat or human NTSR2 cDNA levocabastine and SR 48692 can mobilize intracellular Ca2+ Wnt-C59 more intensively than NTS agonists and phosphorylate Erk1/2 suggesting that NTSR1 and NTSR2 receptors present distinct functional characteristics (Botto et al. (cancer8.info)
  • 2004 In CHO cells transfected with human NTSR2 cDNA both NTSR1 antagonists SR48692 and SR142948A enhance inositol Wnt-C59 phosphate (IP) formation with subsequent [Ca2+] immobilization induce arachidonic acidity discharge and stimulate MAPK activity. (cancer8.info)
  • Human MC were initially divided into three subsets based on the cell surface expression of CD14 and CD16, and recently classified based on CD40 expression ( 2 - 5 ). (frontiersin.org)
  • Stem cells from human exfoliated deciduous teeth (SHED) are multipotent stem cells derived from the pulp tissues of extracted deciduous teeth 1 . (bvsalud.org)
  • Stem cells from human exfoliated deciduous teeth (SHED) (ALLCells, Alameda, CA, USA) were employed in the current study. (bvsalud.org)
  • The primary structure of HH- IRK1 is homologous to that of the IRK1 channel cloned from a mouse macrophage -like cell line. (xenbase.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • pathomechanisms that regulate extravasation of damaging immune cells into surrounding tissues are poorly understood. (nature.com)
  • Mouse over the ending of each transcript gives tissues from which the supporting cDNAs were extracted. (nih.gov)
  • Tissues and Cells. (lu.se)
  • Transfection of GPCR97 into a variety of cell lines conferred an ability to inhibit forskolin-stimulated cAMP formation in response to histamine, but not to acetylcholine or any other biogenic amine. (aspetjournals.org)
  • In this paper we describe the function of SLC19A3, another member of this transporter family most recently cloned, after transient transfection of the cDNA into HeLa cells. (elsevierpure.com)
  • Total RNA was extracted from the cells cultured in KGM after 1, 3 and 7 days. (bvsalud.org)
  • The total RNA was extracted from the cells using RNA extraction kit, RNeasy Mini Kit (Qiagen Inc., Valencia, CA USA) following the protocol provided by the manufacturer. (bvsalud.org)
  • The interaction of FAS/FASL in immature dendritic cells (DCs) induces functional maturation. (biolegend.com)
  • In recent years, much has been learned of the characteristics of immune responses to skin sensitizing chemicals and of the roles played by dendritic cells, cytokines and chemokines. (cdc.gov)
  • Mechanisms of skin sensitization and allergic epidermal Langerhans cells (LC), although other cuta- contact dermatitis neous (dermal) dendritic cells (DC) may also contrib- ute. (cdc.gov)
  • CPA3 functions together with endopeptidases secreted from mast cells such as chymases and tryptases to degrade proteins and peptides, including the apolipoprotein B component of LDL particles and angiotensin I. Upon mast cell activation and degranulation, CPA3, the chymases, and tryptases are released in complexes with heparin proteoglycan. (wikipedia.org)
  • MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis. (genscript.com)
  • The delivery of proteins to the presynaptic terminals of guinea pig retinal ganglion cells by two of the major components of axonal transport, and the subsequent persistence and turnover of those proteins were examined in this study. (shengsci.com)
  • Ganglion cell proteins were radiolabeled by intravitreal injection of radiolabeled am. (shengsci.com)
  • The macrophage inflammatory proteins -1 alpha and -1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. (rndsystems.com)
  • Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8 + and CD4 + T cells, respectively. (rndsystems.com)
  • LHCGR recombinant proteins representing varying lengths of the N-terminal extracellular domain were expressed in Chinese Hamster Ovary cells in suspension culture. (biomedcentral.com)
  • Several viruses require lysosomal cathepsins to cleave structural proteins and thus depend on functional GlcNAc-1-phosphotransferase. (stanford.edu)
  • It is generally accepted that productive infections proceed via interactions between viral envelope molecules and specific receptors on the host cell surface. (wustl.edu)
  • In addition, some of the MMR components participate in recombination and double-strand break repair as well as cell cycle regulation and apoptosis. (aacrjournals.org)
  • A cDNA clone encoding an inwardly-rectifying K-channel (BIR1) was isolated from insulinoma cells. (ox.ac.uk)
  • 6]). The mRNA is expressed in the brain and insulinoma cells. (ox.ac.uk)
  • Metronidazole (Flagyl) is a drug used to treat infections and has been studied in the treatment of cancer [10,11] as a radiosensitizer drug, especially for hypoxic cells [9]. (who.int)
  • tizer drug, especially for hypoxic cells [ 9 ]. (who.int)
  • abstract = "Recently, a new family of facilitative carriers has been cloned consisting of the reduced folate (SLC19A1) and the thiamine (SLC19A2) transporters. (elsevierpure.com)
  • ABSTRACT The in vitro effects of metronidazole on the production of reactive oxygen species by polymorphonuclear (PMN) cells were studied by means of nitroblue tetrazolium and luminol-dependent chemiluminescence. (who.int)
  • The cells were gated on lymphocytes and quadrant markers for the bivariate dot plots were set based on staining with a PE-Cy™7 Mouse IgG1 κ Isotype Control (MOPC-21). (bdbiosciences.com)
  • The development of skin sensitization is associated with, and requires, the activation and clonal expansion of allergen responsive T lymphocytes and it is these cells that orchestrate the cutaneous allergic reaction. (cdc.gov)
  • HHcy in Cbs -/- mice reinforced inflammatory features in Ly6C high MC by upregulating inflammatory TFs ( Ets1 and Tbx21 ) and strengthened lymphocytes functional adaptation in Ly6C low MC by increased expression of CD3, DR3, ICOS, and Fos . (frontiersin.org)
  • Ly6C low MC manifested activated T-cell signaling pathways and potentially can adapt the function of lymphocytes. (frontiersin.org)
  • HHcy reinforced inflammatory feature in Ly6C high MC and strengthened lymphocytes functional adaptation in Ly6C low MC. (frontiersin.org)
  • Prostaglandin E1 (PGE1)-mediated transmembrane signal control systems were investigated in intact murine neuroblastoma cells (clone N1E-115). (shengsci.com)
  • 2012 Nevertheless some research on cellular versions like the murine neuroblastoma cell range N1E-115 and individual cancer of the colon cell range HT-29 showed a big change in the visitors circumstance when the cell got a prolonged contact with saturating dosages of agonist (Souaze et al. (cancer8.info)
  • the height of the top of each intron reflects the relative number of clones supporting this intron. (nih.gov)
  • widely, this buy unreal engine game development blueprints discover all the secrets of unreal engine and create seven fully functional games with, directly with discrete models, is detected defined in the R cDNA JM( 44) and main trait and phytochemicals of constructs are modeled in the round by Rizopoulos( 18). (scoutconnection.com)
  • Amplified cDNA is cloned into expression constructs in one step, enabling expression of thousands of antibodies for rapid functional confirmation. (berkeleylights.com)
  • J Cell Biochem, 2003 Apr 1. (nih.gov)
  • About 4% of cases of diffuse large B-cell cell lymphoma possess BCL2/MYC double translocations, suggesting a synergistic effect of BCL2 and MYC in lymphoma development. (haematologica.org)
  • 8 - 12 Cases of CCND1/MYC double translocation are relatively frequent in mantle cell lymphoma. (haematologica.org)
  • This approach will permit analysis of B cell clonal ontogeny, antibody diversity and lymphoma cell progression and heterogeneity. (lu.se)
  • In order to confirm the oncogenic potential of translation elongation factor-l delta, BALB/c-3T3 cells transformed with cadmium chloride were transfected with the plasmid DNA expressing antisense TEF-I delta mRNA. (cdc.gov)
  • Expression of the antisense translation elongation factor-l delta mRNA resulted in marked reversal of the oncogenic potential of the cadmium -transformed BALB/c- 3T3 cells. (cdc.gov)
  • This was evidenced by a significant reduction in the number of anchor- age independent colonies developing from the transformed cells expressing antisense mRNA for translation elongation factor-1 delta compared with the vector alone-transfected control cells. (cdc.gov)
  • Furthermore, sub-cutaneous injection of the antisense mRNA expressing transformed BALB/c-3T3 cells resulted in a significant reduction in the onset as well as the size of tumors in athymic nude mice. (cdc.gov)
  • CD2 mRNA was expressed in murine thymus and spleen, and in the T cell line EL4. (ox.ac.uk)
  • RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2 and, Hes1) as well as stem cell marker (Nanog). (bvsalud.org)
  • It will also facilitate structural and functional studies of immunoglobulins as well as the rapid construction of chimeric antibodies. (lu.se)
  • We annotate structural defects or features in 6 cDNA clones. (nih.gov)
  • The majority of cancer cells rely on reactivation of the enzyme telomerase or activation of the Alternative Lengthening of Telomeres pathway (ALT) to promote telomere elongation. (oncotarget.com)
  • We envisioned that enzyme-activatable cMOs could overcome these limitations, as the triggering enzymes could be expressed in individual cell populations using cis regulatory elements. (cdc.gov)
  • NfsB is a dimeric flavoprotein enzyme that has broad electron acceptor specificity 15 and has been used in conjunction with prodrugs to selectively ablate cells in vertebrates. (cdc.gov)
  • Knowledge on the expression analysis of Notch signaling pathway molecules in SHED cultured in KGM could highlight its involvement in controlling the biological activity of these stem cells, particularly during odontogenesis and other developmental process. (bvsalud.org)
  • A new mast cell subtype reported to appear in mucosa is implicated in allergic inflammation and these mast cells have high levels of CPA3. (wikipedia.org)
  • Low density lipoprotein degradation by secretory granules of rat mast cells. (wikipedia.org)
  • IL-17F is expressed in activated CD4 + T cells, basophils, and mast cells, three important cell types involved in allergic airway inflammation [ 3 ]. (hindawi.com)
  • Clones encoding the murine homologue were isolated from a murine T helper cell cDNA library. (ox.ac.uk)
  • Functional studies revealed increased expression of ICAM-1 on induced patient-derived endothelial cells (iECs) and of β2-integrins on patient neutrophils that increase neutrophil adhesion and vascular transendothelial migration (TEM). (nature.com)
  • We characterize the role of increased Lyn kinase activity in neutrophils, endothelial cells and lesional liver biopsies and utilized an iPSC-derived endothelial cell platform for disease modeling of neutrophilic vasculitis and to screen and evaluate drug efficacy. (nature.com)
  • Cancer cells secrete matrix metalloproteinases to remodel the extracellular matrix, which enables them to overcome tissue barriers and form metastases. (nature.com)
  • One of the most life-threatening aspects of cancer is the ability of transformed cells to invade into the extracellular matrix (ECM) and neighboring tissue to form metastases 1 , 2 . (nature.com)
  • Matrix metalloproteinases (MMPs) play a critical role in progression of cancer by degrading and remodeling ECM, enabling cells to overcome tissue barriers, travel within the circulatory system before extravasating to produce a secondary tumor 3 , 4 . (nature.com)
  • 7. Roberds SL and Tamkun MM (1991) Cloning and tissue-specific expression of five voltage-gated potassium channel cDNAs expressed in rat heart. (aspetjournals.org)
  • Monocytes (MC) are bone marrow (BM) derived mononuclear phagocytes that play an important role in innate immune response and are the major immune cell population in chronic tissue inflammatory ( 1 , 2 ). (frontiersin.org)
  • Tissue infiltration by activated innate immune cells, including monocytes, macrophages, and neutrophils, if untreated, leads to rapidly progressive organ injury, that can involve many organ systems. (nature.com)
  • CHROMOVERT TECHNOLOGY is a newly published research tool for rapid creation of stable cell lines. (genelink.com)
  • Metronidazole selectively radiosensitizes hypoxic cells without influencing the radiation response of normal well-oxygenated cells [5,7-9]. (who.int)
  • Metronidazole selectively radiosensitizes of metronidazole (in a range of therapeutic hypoxic cells without influencing the ra- concentrations) on PMN-generated free diation response of normal well-oxygenated radicals during phagocytosis activity in cells [ 5,7-9 ]. (who.int)
  • Conclusions Bcl2 , Myc and Ccnd1 or Bcl2 , Myc and CCND3 synergistically transformed mouse primary B cells into aggressive malignant cells. (haematologica.org)
  • At negative membrane potentials, TRPM4 allows Na + entry into the cell, leading to cellular membrane depolarization. (frontiersin.org)
  • 3H]Iloprost specifically bound to the membrane of CHO cells stably expressing the cDNA with a Kd of 3.3 nM. (drugbank.com)