• MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis. (genscript.com)
  • A number of pS2 similar peptides, designated as P-domain peptides, have been described, notably the porcine ( PSP ), murine ( mSP ), and human (hSP) spasmolytic polypeptides, which correspond to duplicated pS2 proteins. (xenbase.org)
  • We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells. (amsbio.com)
  • We and other groups discovered the human IL-17F gene from a human EST sequence, a genomic DNA clone, and T-cell cDNA sequences in 2001 [ 3 , 8 , 9 ]. (hindawi.com)
  • The following PBX2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
  • These sequences represent the protein coding region of the PBX2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. (genscript.com)
  • ORF sequences can be delivered in our standard vector, pcDNA3.1 + /C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. (genscript.com)
  • Prediction of the coding sequences of unidentified human genes. (drugbank.com)
  • The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1. (drugbank.com)
  • Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (nih.gov)
  • For each isolated cell, they separately clone the two genes encoding the heavy and light chains that compose an antibody, and that vary in sequence from cell to cell. (the-scientist.com)
  • Once researchers have cloned the pairs of heavy- and light-chain genes naturally found in the cells, they can produce recombinant versions of each antibody in the lab. (the-scientist.com)
  • The issue with it is that there are a lot of steps for each cell to clone those antibody genes. (the-scientist.com)
  • Using PCR, researchers then amplify the heavy and light chain genes from antibodies of interest, clone them into expression vectors, and sequence them. (the-scientist.com)
  • But rather than cloning the antibody heavy- and light-chain genes from each cell, as in a typical expression cloning set-up, Connors's group distributed the isolated cells in 384-well plates, where they cultured them in the presence of feeder cells and signaling molecules that help to keep the B cells alive and also stimulate them to secrete antibodies. (the-scientist.com)
  • Known as the hORFeome v5.1, it contains over 15,000 individual clones representing over 12,000 unique genes. (horizondiscovery.com)
  • The human IL-3 gene encodes a protein 152 amino acids long, and the naturally occurring IL-3 is glycosylated. (wikipedia.org)
  • The cloned cDNA encodes a putative peptide of 490 amino acids with seven potential transmembrane domains. (jci.org)
  • Cloning, characterization, and expression of a human calcitonin receptor from an ovarian carcinoma cell line. (jci.org)
  • Transfection of this cDNA into COS cells resulted in expression of receptors with high affinity for salmon CT (Kd = approximately 0.44 nM) and for human CT (Kd = approximately 5.4 nM). (jci.org)
  • Title: Expression level of pre-B-cell leukemia transcription factor 2 (PBX2) as a prognostic marker for gingival squamous cell carcinoma. (genscript.com)
  • This technique, commonly referred to as expression cloning, "is powerful because you're not limited-any B cell that you can isolate, in any context, you can make an antibody from," Wilson says. (the-scientist.com)
  • The expression vectors are transfected into cells, and the resulting antibodies are purified and tested for activity. (the-scientist.com)
  • loss of expression is associated with increased metastatic potential of prostate cancer cells [RGD, Feb 2006]. (genscript.com)
  • Expression of KAI1/CD82 and MRP-1/CD9 in transitional cell carcinoma of bladder. (genscript.com)
  • The mouse pS2 gene is highly expressed in the stomach mucosa cells, whereas no pS2 gene expression could be detected in the mouse mammary gland, even during postnatal development processes dependent on growth factors or hormones. (xenbase.org)
  • Strong association between c-myb and oestrogen-receptor expression in human breast cancer. (xenbase.org)
  • Expression of the oestrogen regulated pNR-2 mRNA in human breast cancer: relation to oestrogen receptor mRNA levels and response to tamoxifen therapy. (xenbase.org)
  • Expression of the pNR-2/pS2 protein in diverse human epithelial tumours. (xenbase.org)
  • Human thyroid peroxidase (TPO) isoforms, and TPO-2: analysis of protein expression in Graves' tissue. (rusmedserv.com)
  • Chromosomal gene structure of human myeloperoxidase and regulation of its expression by granulocyte colony-stimulating factor. (rusmedserv.com)
  • It has been also shown that CXCR4 signalling regulates the expression of CD20 on B cells. (wikidoc.org)
  • Expression of this receptor in cancer cells has been linked to metastasis to tissues containing a high concentration of CXCL12, such as lungs, liver and bone marrow. (wikidoc.org)
  • [12] [13] However, in breast cancer where SDF1/CXCL12 is also expressed by the cancer cells themselves along with CXCR4, CXCL12 expression is positively correlated with disease free (metastasis free) survival. (wikidoc.org)
  • Cloning, expression, and differentiation-dependent regulation of SMAF1 in adipogenesis. (nih.gov)
  • ALiCE® is a radically different approach to cell-free expression and a kit unlike any others. (amsbio.com)
  • Cloning and functional expression of the cDNA encoding an inwardly-rectifying potassium channel expressed in pancreatic beta-cells and in the brain. (ox.ac.uk)
  • A genome-scale library of Gateway-adapted human ORF clones, the CCSB Human ORFeome Library (aka hORFeome V5.1) is ideal for transferring thousands of open reading frames into a variety of protein expression systems. (horizondiscovery.com)
  • Dharmacon is a distributor of multiple gene expression clone collections (cDNAs and ORFs). (horizondiscovery.com)
  • OriGene offers a range of tools to analyze CD markers including cDNA clones, antibodies, overexpression cell lysates and more. (origene.com)
  • Characterization of cDNA clones for human myeloperoxidase: predicted amino acid sequence and evidence for multiple mRNA species. (rusmedserv.com)
  • Sakamaki K, Tomonaga M, Tsukui K, Nagata S. Molecular cloning and characterization of a chromosomal gene for human eosinophil peroxidase. (rusmedserv.com)
  • Isolation and characterization of a cDNA clone for porcine thyroid peroxidase. (rusmedserv.com)
  • Interleukin 3 (IL-3) is a protein that in humans is encoded by the IL3 gene localized on chromosome 5q31.1. (wikipedia.org)
  • In heterotrimeric G-protein signalling, cell surface receptors (GPCRs) are coupled to membrane-associated heterotrimers comprising a GTP-hydrolysing subunit G-alpha and a G-beta/G-gamma dimer. (embl-heidelberg.de)
  • Mammalian Purkinje-cell protein-2 (Pcp2). (embl-heidelberg.de)
  • It may function as a cell-type specific modulator for G protein-mediated cell signalling. (embl-heidelberg.de)
  • Interaction of heterotrimeric G protein Galphao with Purkinje cell protein-2. (embl-heidelberg.de)
  • Pcp2 (Purkinje cell protein-2) was identified as a partner for Galphao in this system. (embl-heidelberg.de)
  • Long-term activation of protein kinase c causes chronic Na/H antiporter stimulation in cultured proximal tubule cells. (jci.org)
  • To examine the role of protein kinase C as a chronic regulator of proximal tubule Na/H antiporter activity, the effect of phorbol 12-myristate 13-acetate (PMA) on the Na/H antiporter was studied in cultured proximal tubule cells. (jci.org)
  • Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT). (genscript.com)
  • We have now cloned a mouse homolog of the human pS2 cDNA to dispose of an animal model to study the pS2 protein function, which remains unknown at the present time. (xenbase.org)
  • Human thyroid peroxidase: complete protein sequence, chromosome mapping, and identification two alternately spliced mRNAs. (rusmedserv.com)
  • The biological effects of Epo are mediated by the Erythropoietin receptor (Epo R). At the protein sequence level, the human Epo R is approximately 83% identical to the mouse protein. (rndsystems.com)
  • Human liver protein map: a reference database established by microsequencing and gel comparison. (drugbank.com)
  • Plasma and red blood cell protein maps: update 1993. (drugbank.com)
  • C-X-C chemokine receptor type 4 ( CXCR-4 ) also known as fusin or CD184 (cluster of differentiation 184) is a protein that in humans is encoded by the CXCR4 gene . (wikidoc.org)
  • [3] Ubiquitin is a small (76-amino acid) protein highly conserved among eukaryotic cells. (wikidoc.org)
  • To clone a human CTR (hCTR), a BIN-67 cDNA library was screened using a cDNA probe from a porcine renal CTR (pCTR) that we recently cloned. (jci.org)
  • The structural differences may account for observed differences in binding affinity between the porcine renal and human ovarian CTRs. (jci.org)
  • We explored whether LPL affects (a) lipoprotein transport across bovine aortic endothelial cells or (b) lipoprotein binding to subendothelial cell matrix (retention). (jci.org)
  • Molecular cloning of cDNAs encoding bovine and human lactoperoxidase. (rusmedserv.com)
  • Frank S, Munz B, Werner S: The human homologue of a bovine non-selenium glutathione peroxidase is a novel keratinocyte growth factor-regulated gene. (drugbank.com)
  • Estrogen-responsive element of the human pS2 gene is an imperfectly palindromic sequence. (xenbase.org)
  • Sequence of the pS2 mRNA induced by estrogen in the human breast cancer cell line MCF-7. (xenbase.org)
  • Cloning and sequence analysis of the human salivary peroxidase-encoding cDNA. (rusmedserv.com)
  • Derwahl M, Seto P, Rapoport B. Complete nucleotide sequence of the cDNA for thyroid peroxidase in FRTL5 rat thyroid cells. (rusmedserv.com)
  • Nucleotide sequence of the cDNA encoding mouse thyroid peroxidase. (rusmedserv.com)
  • The sequence homology with KATP-1 suggests that BIR1 is a subunit of a brain and beta-cell KATP channel. (ox.ac.uk)
  • Dharmacon has not sequence verified each individual clone from these collections. (horizondiscovery.com)
  • Subsequently, the investigators identified a specific clone as containing pro-proliferative effects that was sensitive to neutralization by the soluble IL-21R. (intechopen.com)
  • These clones are derived from fully-sequenced Mammalian Gene Collection (MGC) full-length cDNAs and subsequently cloned into a Gateway recombinational entry vector. (horizondiscovery.com)
  • Human FAS, amino acids (Arg17-Asn173) (Accession# NM_000043), was expressed in 293E cells. (biolegend.com)
  • We have previously shown that the human pS2 gene, which codes for a secreted peptide of 60 amino acids, is expressed in a number of human carcinomas, including carcinomas of the breast, the pancreas , and the large bowel. (xenbase.org)
  • Human IL-3 was first cloned in 1986 and since then clinical trials are ongoing. (wikipedia.org)
  • 1997. Metabolic activation of aromatic amines by human pancreas. (cdc.gov)
  • However, IL-3 has also been shown to be produced in IgG+ B cells and may be involved in earlier antibody isotype switching. (wikipedia.org)
  • In this approach, researchers first collect antibody-producing cells from mice exposed to an antigen of interest and fuse them to myeloma cells, forming so-called hybridomas that can survive for a long time in culture. (the-scientist.com)
  • By screening single hybrid cells for those that produce the antibody of interest, researchers can develop a cell line generating virtually unlimited amounts of highly specific antibodies. (the-scientist.com)
  • It would be extremely laborious to use this strategy to try to identify an antibody against an antigen of interest using the blood of a person without an active immune response, he says, because millions of memory B cells are present in the blood, each of which is uniquely specific for a different antigen. (the-scientist.com)
  • CHARTING THE COURSE: B cells grow in culture in individual wells over a layer of feeder cells, and culture media from cells is screened for antibody-binding specificity or neutralizing activity. (the-scientist.com)
  • The immortalization of antibody-producing cells from patients infected with HIV can be inefficient, and even when cells are successfully immortalized, some clones tend to be unstable. (the-scientist.com)
  • IL-3 receptors can be found on a variety of cell types including many immature myelomonocytic cells in the hemopoietic system such as hemopoietic progenitor cells, as well as certain myeloid progenitors, basophils, and eosinophils. (wikipedia.org)
  • A human ovarian small cell carcinoma line (BIN-67) expresses abundant calcitonin (CT) receptors (CTR) (143,000 per cell) that are coupled, to adenylate cyclase. (jci.org)
  • CXCR4 is one of several chemokine receptors that HIV can use to infect CD4+ T cells . (wikidoc.org)
  • CXCR4 is upregulated during the implantation window in natural and hormone replacement therapy cycles in the endometrium, producing, in presence of a human blastocyst , a surface polarization of the CXCR4 receptors suggesting that this receptor is implicated in the adhesion phase of human implantation . (wikidoc.org)
  • Platelet and endothelial cell adhesion molecule 1. (invivogen.com)
  • We have recently identified, for the first time, that bronchial epithelial cells are its novel cell source in response to IL-33 via ST2-ERK1/2-MSK1 signaling pathway. (hindawi.com)
  • Embryo-like models with spatially organized morphogenesis and structure of all defining embryonic and extra-embryonic tissues of the post-implantation human conceptus (that is, the embryonic disc, the bilaminar disc, the yolk sac, the chorionic sac and the surrounding trophoblast layer) remain lacking 1 , 2 . (nature.com)
  • Much of this process relies on the morphogenesis of the extra-embryonic tissues and the effect this has on the organization of embryonic cells. (nature.com)
  • Mechanochemical Principles of Spatial and Temporal Patterns in Cells and Tissues. (mpi-cbg.de)
  • Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
  • Complete cDNA cloning in human and partial cloning in Chinese hamster ovary cells. (nih.gov)
  • IL-3 is produced by T cells only after stimulation with antigens or other specific impulses. (wikipedia.org)
  • FAS was initially purified and cloned from SKW6.4 cells and a cDNA library of human T cell lymphoma KT-3 cells. (biolegend.com)
  • CD31/PECAM-1 impacts engraftment, growth and spread of mantle cell lymphoma cells and positively correlates with extramedullary involvement. (invivogen.com)
  • Antibodies derived from animals, however, are problematic for use as therapeutics, because the molecules can trigger immune responses in humans. (the-scientist.com)
  • Although all of these approaches have been valuable for identifying new antibodies and characterizing human immune responses, they are time-consuming and complicated. (the-scientist.com)
  • While previous researchers have obtained HIV-neutralizing antibodies using fluorescent probes to pull out antigen-specific memory B cells from a sample of blood, Connors and his team wanted to discover new HIV-neutralizing antibodies, without being limited only to those that are specific for known antigens. (the-scientist.com)
  • The interaction of FAS/FASL in immature dendritic cells (DCs) induces functional maturation. (biolegend.com)
  • It is uniquely expressed in cerebellar Purkinje cells and in retinal bipolar neurons. (embl-heidelberg.de)
  • Pcp2 is expressed in cerebellar Purkinje cells and retinal bipolar neurons, two locations where Galphao is also expressed. (embl-heidelberg.de)
  • Mouse naive embryonic stem cells have recently been shown to give rise to embryonic and extra-embryonic stem cells capable of self-assembling into post-gastrulation structured stem-cell-based embryo models with spatially organized morphogenesis (called SEMs) 3 . (nature.com)
  • Here we extend those findings to humans using only genetically unmodified human naive embryonic stem cells (cultured in human enhanced naive stem cell medium conditions) 4 . (nature.com)
  • CXCR4's ligand SDF-1 is known to be important in hematopoietic stem cell homing to the bone marrow and in hematopoietic stem cell quiescence . (wikidoc.org)
  • Peripheral blood stem cell mobilization is very important in hematopoietic stem cell transplantation (as a recent alternative to transplantation of surgically harvested bone marrow ) and is currently performed using drugs such as G-CSF . (wikidoc.org)
  • It is a very efficient inducer of hematopoietic stem cell mobilization in animal and human studies. (wikidoc.org)
  • Interleukin 3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. (wikipedia.org)
  • The cytokine was originally discovered via the observation that it induced the synthesis of 20alpha-hydroxysteroid dehydrogenase in hematopoietic cells and termed it interleukin-3 (IL-3). (wikipedia.org)
  • IL-3 is produced as a monomer by activated T cells, monocytes/macrophages and stroma cells. (wikipedia.org)
  • In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g. (wikipedia.org)
  • Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. (xenbase.org)
  • Northern analysis with the hCTR cDNA probe indicated a single transcript of approximately 4.2 kb. (jci.org)
  • Identification of an immunodominant region recognized by human autoantibodies in a three-dimensional model of thyroid peroxidase. (rusmedserv.com)
  • Proximal tubule cells exposed to PMA for 2 h demonstrated a two- to threefold increase in Na/H antiporter mRNA (mRNANa/H) abundance 4 h later. (jci.org)
  • 6]). The mRNA is expressed in the brain and insulinoma cells. (ox.ac.uk)
  • Leukemia stem cells (LSCs)play a critical role in the initiation, progression and treatment resistance in AML. (origene.com)
  • However, we know less about the biological effects of IL-21 and its signaling mechanisms in leukemia cells and monocytes. (intechopen.com)
  • In this chapter, we will focus on IL-21's biological effects and signaling pathways as well as discuss the potential implications and applications of IL-21 in leukemia cells. (intechopen.com)
  • These three cytokines are differentially expressed in activated CD4 + T cells. (hindawi.com)
  • Its roles include regulation of proliferation, promotion of immune system and activation of apoptosis in B cells. (intechopen.com)
  • Involved in redox regulation of the cell. (drugbank.com)
  • Inhibition of cytotoxic effect of FASL on Jurkat cells in the presence of recombinant human FAS. (biolegend.com)
  • ED 50 = 10 - 50 ng/ml, corresponding to a specific activity of 0.02 - 0.1 x 10 6 units/mg, as determined by inhibition of apoptotic cell death induced by FASL on Jurkat cells. (biolegend.com)
  • The cDNAs for Epo have been cloned from many species. (rndsystems.com)
  • MicroRNA?1915?3p prevents the apoptosis of lung cancer cells by downregulating DRG2 and PBX2. (genscript.com)
  • In these cells, IL-21 does not promote proliferation but enhances apoptosis and chemotaxis. (intechopen.com)
  • IL-3 is mainly produced by activated T cells with the goal of initiating proliferation of various other immune cell types. (wikipedia.org)
  • Interleukin-21 (IL-21) is produced by activated T cells and it plays many diverse roles by regulating the functions of normal and abnormal cells. (intechopen.com)
  • Interleukin-21 (IL-21) was first identified in 2000 through screening of a cDNA library from CD3+ human T cells [ 1 ]. (intechopen.com)
  • IL-3 is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response. (wikipedia.org)
  • G-CSF is a growth factor for neutrophils (a common type of white blood cells), and may act by increasing the activity of neutrophil-derived proteases such as neutrophil elastase in the bone marrow leading to proteolytic degradation of SDF-1. (wikidoc.org)
  • More recently, Wilson and others have devised ways to isolate single, antigen-specific B cells from the blood of human donors with active immune responses against a particular pathogen or vaccine. (the-scientist.com)
  • E3 ubiquitin ligase RNF170 inhibits innate immune responses by targeting and degrading TLR3 in murine cells. (invivogen.com)
  • Role of intracellular trafficking of thyroperoxidase and involvement generated at the apical surface of thyroid cells in covalent heme binding. (rusmedserv.com)
  • It induces proliferation and differentiation in both early pluripotent stem cells and committed progenitors. (wikipedia.org)
  • IL-3 also induces various effector functions in both immature and mature cells that more precisely modulate the body's defense against microbial pathogens. (wikipedia.org)
  • IL-3/Receptor complex induces JAK2/STAT5 cell signalization pathway. (wikipedia.org)
  • The present invention provides a simple and robust human liver cell-based system in which persistent hepatitis C infection, persistent hepatitis B infection or ethanol exposure induces a clinical Prognostic Liver Signature (PLS) high-risk gene signature. (sumobrain.com)
  • Such human fully integrated and complete SEMs recapitulate the organization of nearly all known lineages and compartments of post-implantation human embryos, including the epiblast, the hypoblast, the extra-embryonic mesoderm and the trophoblast layer surrounding the latter compartments. (nature.com)
  • Although it is possible to culture structures derived from human blastocysts ex vivo, these cultures do not recapitulate the events and structural organization of the in vivo embryos 6 ( Supplementary Information ). (nature.com)
  • IL-3 is a T cell-derived, pluripotent and hematopoietic factor required for survival and proliferation of hematopoietic progenitor cells. (wikipedia.org)
  • The production of Epo by kidney cells is increased in response to hypoxia or anemia. (rndsystems.com)
  • IL-3 is capable of stimulating differentiation of immature myelomonocytic cells causing changes to the macrophage and granulocyte populations. (wikipedia.org)
  • This technique is best for "when you have an enriched population of antigen-specific cells-they're not there, except for a short time after vaccination or infection," says Wilson. (the-scientist.com)
  • The human IL-3 gene is located on chromosome 5, only 9 kilobases from the GM-CSF gene, and its function is quite similar to GM-CSF. (wikipedia.org)
  • Regional localization of the thyroid peroxidase to human chromosome 2pter-p12. (rusmedserv.com)
  • UDS activity in the rat liver of the human carcinogens benzidine and 4-aminobiphenyl, and the rodent carcinogens 3,3'-dichlorobenzidine and direct black 38. (cdc.gov)
  • IL-17F is expressed in activated CD4 + T cells, basophils, and mast cells, three important cell types involved in allergic airway inflammation [ 3 ]. (hindawi.com)
  • [9] WHIM like mutations in CXCR4 were recently identified in patients with Waldenstrom's macroglobulinemia , a B-cell malignancy. (wikidoc.org)
  • In the early 1960s Ginsberg and Sachs discovered that IL-3 is a potent mast cell growth factor produced from activated T cells. (wikipedia.org)
  • You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart. (genscript.com)
  • The Center for Cancer Systems Biology of the Dana-Farber Institute created a collection of human open reading frames (ORFs) cloned into a Gateway-entry vector, known as the CCSB Human ORFeome Collection. (horizondiscovery.com)
  • Ovarian cancer cell heparan sulfate 6-O-sulfotransferases regulate an angiogenic program induced by heparin-binding epidermal growth factor (EGF)-like growth factor/EGF receptor signaling. (nih.gov)
  • In conjunction with other β common chain cytokines GM-CSF and IL-5, IL-3 works to regulate the inflammatory response in order to clear pathogens by changing the abundance of various cell populations via binding at the interleukin-3 receptor. (wikipedia.org)
  • Activated T cells can either induce their own proliferation and differentiation (autocrine signaling), or that of other T cells (paracrine signaling) - both involve IL-2 binding to the IL-2 receptor on T cells (upregulated upon cell activation, under the induction of macrophage-secreted IL-1). (wikipedia.org)
  • The signal transmission is ensured by high affinity between cell surface interleukin-3 receptor and IL-3. (wikipedia.org)
  • Drugs that block the CXCR4 receptor appear to be capable of "mobilizing" hematopoietic stem cells into the bloodstream as peripheral blood stem cells . (wikidoc.org)