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  • HeLa
  • We purified this polypeptide from HeLa cells using a Western blot assay, and named it p54nrb (for nuclear RNA-binding protein, 54 kDa). (cshl.edu)
  • isolation
  • The isolation of this clone will permit investigations into the causes for GALC deficiency in humans and available animal models, development of more accurate tests for patient and carrier identification, and evaluation of methods for effectively treating GALC deficiency, initially using the animal models. (uniprot.org)
  • Gene
  • T-cell activation with concanavalin A plus interleukin-2 induced spleen cells to express this gene with kinetics correlating with the acquisition of cytolytic capacity. (sciencemag.org)
  • Because of its ability to efficiently transduce cells, and mediate genomic integration, this hybrid vector may be useful for gene therapy applications. (medsci.org)
  • To facilitate high-level transcription of HCV RNA, Vero-E6 cells were transfected with pHCV and then infected with recombinant vaccinia virus (vTF7-3) containing the T7 RNA polymerase gene. (virosin.org)
  • In the brain, PAF participates im physiological mechanisms such as synaptic transmission, long-term potentiation, memory formation, proliferation and differentiation of neural cells, regulation of gene expression, and chemotaxis. (springer.com)
  • The product of this gene is a prenylated and palmitoylated phosphoprotein that associates with the cytoplasmic face of plasma membranes and is implicated in plasma membrane dynamics in neurons and other cell types. (wikipedia.org)
  • The Drosophila dishevelled gene encodes a cytoplasmic phosphoprotein that regulates cell proliferation. (wikipedia.org)
  • Hepatitis C Vir
  • A Vero-E6 culture system was developed to assay Hepatitis C virus (HCV) replication by plasmid (pHCV) transfection,which contains a T7 promoter at the 5'end, full-length cDNA of the HCV genome and a T7 terminator. (virosin.org)
  • sequences
  • Based on the deduced amino acid sequences of known mast cell tryptases of other species, the gerbil mast cell tryptase (gMCT) was highly similar to mouse mast cell protease (mMCP)-7, and seems to be translated as a prepro-enzyme with 25 amino acids of signal and activation peptides and 245 amino acids of mature enzyme. (biochemj.org)
  • These cDNAs have identical coding sequences but differ as a result of alternative splicing in the 5' untranslated region. (cshl.edu)
  • protein
  • Two overlapping clones contained the total protein coding region, while additional clones and PCR amplification were needed to obtain the complete 3' end of the cDNA. (uniprot.org)
  • cDNAs encoding p54nrb were cloned with probes derived from partial sequence of the purified protein. (cshl.edu)
  • Cell surface molecule CD2 is a 45- to 58-kDa type I integral protein expressed on virtually all T lineage and NK cells ( 1 ). (jimmunol.org)
  • The Cagvasa cDNA is 2771 nt (nucleotide) and encodes a 701-aa (amino acid) protein (Cag Vasa) that possesses 14 RGG-repeats and all the eight conserved motifs of Vasa proteins. (ihb.ac.cn)
  • To develop a tool for analyzing fish germ cells, we generated alpha Vasa, a rabbit antibody against a recombinant protein of 310-aa N-terminal Cag Vasa. (ihb.ac.cn)
  • The protein is primarily expressed on sinusoidal endothelial cells of liver, spleen, and lymph node. (wikipedia.org)
  • The beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. (wikipedia.org)
  • adhesion
  • Nevertheless, engagement of CD2 to CD58 in humans, or to CD48 in rodents, facilitates adhesion between T cells and APC and is proposed to promote the formation of an optimal intercellular membrane spacing (∼140 Å) suitable for TCR recognition of a peptide Ag bound to MHC ( 6 ). (jimmunol.org)
  • Many reports suggest that epithelial cell adhesion molecule (EpCAM) is a useful marker for cancer stem cells in hepatocellular carcinoma (HCC). (hindawi.com)
  • murine
  • A new serine protease was encoded by a clone isolated from a murine cytotoxic T-lymphocyte complementary DNA library by an RNA-hybridization competition protocol. (sciencemag.org)
  • This hybrid vector, AdLTR-luc, was created by adding two elements from Moloney murine leukemia virus (MoMLV) flanking the luciferase cDNA into an E1/E3-deleted, replication deficient serotype 5 adenovirus vector (Zheng et al. (medsci.org)
  • In murine T cells, a significant fraction of CD2 associates with membrane lipid rafts. (jimmunol.org)
  • lipid
  • Following incubation of rat CD2-expressing cells with radioactive-labeled palmitic acid, or using CD2 mutants with Cys 226 and Cys 228 replaced by alanine residues, we found no evidence that rat CD2 was subjected to lipid modifications that could favor the translocation to lipid rafts, discarding palmitoylation as the principal mechanism for raft addressing. (jimmunol.org)
  • dilution
  • In contrast to frequencies of 1 in 10 5 -10 6 as measured by limiting dilution analysis, estimates of the T cell frequencies expressing MBPp85-99-associated TCR chain transcripts were as high as 1 in 300. (rupress.org)
  • chromosome
  • Classically, following MoMLV entry into a cell, the RNA genome is reverse transcribed into DNA and integration of this DNA copy into a host cell chromosome occurs as an essential step in viral replication ( 9 - 11 ). (medsci.org)
  • proteins
  • All PCR products including those that are difficult to clone by other PCR cloning methods or that may be unstable or code for potentially detrimental proteins can be cloned and maintained as single copy to ensure their stability. (bio-medicine.org)
  • The amino terminal extension of the deduced amino acid sequence of this clone had a characteristic feature of the transit peptides of plastid-destined proteins. (oup.com)
  • downstream
  • The purpose of the current study was to determine if the MoMLV element downstream of the luciferase cDNA was also broken when integration occurred. (medsci.org)
  • Southern hybridizations after Nco I digestion suggested that there was a break in both MoMLV elements, upstream and downstream of the luciferase cDNA. (medsci.org)
  • mucosal
  • Throughout the course of infection, enzyme-histochemically detectable tryptase activity was limited to mucosal mast cells. (biochemj.org)
  • Since mucosal mast cells of other rodents, including mice and rats, do not express tryptases, this is the first report of rodent mast cell tryptase expressed in the intestinal mucosa. (biochemj.org)
  • homolog
  • To provide a germ cell marker, we cloned and characterized Cagvasa, a vasa homolog from the gibel carp Carassius auratus gibelio. (ihb.ac.cn)
  • receptors
  • Many cell membrane receptors, such as the TCR, coreceptors, and accessory molecules associate within these microdomains upon cell activation. (jimmunol.org)
  • Neural cells possess plasma membrane and intracellular receptors. (springer.com)
  • Moreover
  • Moreover, ligation of CD2 with CD58 augments the IL-12 responsiveness of activated T cells ( 9 ), and can also reverse T cell anergy induced by B7 blockage ( 10 ). (jimmunol.org)
  • Moreover, the ability to directly measure frequencies of MBPp85-99-reactive T cells allowed us to functionally examine the response of autoreactive T cells to antigen. (rupress.org)
  • liver
  • Complementary transcripts were detected in cytotoxic T lymphocytes, spleen cells from nude mice, a rat natural killer cell leukemia, and in two of eight T-helper clones (both cytotoxic), but not in normal mouse kidney, liver, spleen, or thymus, nor in several tested T- and B-cell tumors. (sciencemag.org)
  • lineage
  • The basis for animal reproduction and reproductive biotechnology is germ cells that are segregated from the somatic lineage early in embryonic development and produce sperm and eggs for germline transmission between generations. (ihb.ac.cn)
  • Bone resorbing osteoclasts are terminally differentiated polykaryons of the monocyte/macrophage lineage that are specialized cells responsible for physiological bone resorption as well as pathologic bone loss. (pubmedcentralcanada.ca)
  • In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g. (wikipedia.org)
  • marker
  • At first, EpCAM was reported as a marker of the cancer stem cell in the pancreatic carcinoma and the breast cancer. (hindawi.com)
  • Therefore, we thought that EpCAM could become a useful cancer stem cell marker in a HCC. (hindawi.com)
  • transcripts
  • This transfection-based cell culture system produced high levels of HCV genome (107-108 copies/mL) detectable by real-time quantitative PCR and the production of HCV RNA transcripts was confir med by RT-PCR. (virosin.org)
  • In contrast to frequencies of one in 10 5 to 10 6 as measured by LDA, estimates of the T cell frequencies expressing MBPp85-99 associated TCR chain transcripts were as high as 1 in 300. (rupress.org)
  • Proteins
  • Clathrin coated vesicles enable neurotransmitter receptors and other proteins to be endocytosed or taken up across neuronal membranes and across the membranes of other types of cells. (wikipedia.org)
  • The Atg proteins regulate autophagy, which is a lysosomal degradation pathway required for maintaining cell health, surviving periods of nutrient deprivation and also plays a role in cancer, neurodegeneration and immune responses to a diverse range of pathogens. (wikipedia.org)
  • Interferons (IFNs) are a group of signaling proteins made and released by host cells in response to the presence of several pathogens, such as viruses, bacteria, parasites, and also tumor cells. (wikipedia.org)
  • IFNs belong to the large class of proteins known as cytokines, molecules used for communication between cells to trigger the protective defenses of the immune system that help eradicate pathogens. (wikipedia.org)
  • genes
  • Granulysin is a cytolytic and proinflammatory molecule first identified by a screen for genes expressed "late" (3-5 days) after activation of human peripheral blood mononuclear cells. (wikipedia.org)
  • Another cellular enzyme, RNAse L-also induced by interferon action-destroys RNA within the cells to further reduce protein synthesis of both viral and host genes. (wikipedia.org)
  • embryonic
  • In vitro model systems, based on the differentiation of embryonic stem (ES) cells, provide an alternative means of identifying and characterizing these populations. (biologists.org)
  • Hela
  • WIPI2 mRNA is readily detectable in several commonly used laboratory cell lines (HEK293A, HeLa, A431) and several cancer cell lines, while WIPI1 expression is limited to cancer cells. (wikipedia.org)
  • experimental
  • The ability to progress via a definitive ectoderm intermediate will underpin the development of rational and efficient methodologies for the derivation of specific ectodermal cell populations from pluripotent cells in culture for use as experimental models or to produce cells with therapeutic applications. (biologists.org)
  • Transient
  • Recognition and analysis of these transient cell populations has been hampered by technical difficulties in accessing them in vivo. (biologists.org)
  • Using a previously established mouse ES-cell-based system that recapitulates the development of the ectoderm lineage we have identified a transient population that is consistent with definitive ectoderm. (biologists.org)
  • mammalian
  • The formation and differentiation of multipotent precursors underlies the generation of cell diversity during mammalian development. (biologists.org)
  • transcription
  • It has been associated with heterochromatin and PML-NBs (Promyelocytic Leukaemia nuclear bodies) and has been implicated in many nuclear processes including transcription and cell cycle regulation. (wikipedia.org)
  • dorsal
  • Thus, in X. laevis embryos, the ectoderm is patterned by the secretion of BMP antagonists from the dorsal blastopore lip, or Spemann's organizer: BMP signalling is active ventrally, and induces the formation of surface ectoderm, whereas dorsal BMP signalling is inhibited, and the cells adopt a neural fate ( Hemmati-Brivanlou and Melton, 1997 ). (biologists.org)
  • fluorescence
  • Adjustments in the Ca2+ focus had been indicated as F/F, where F was the fluorescence strength when cells had been at rest, and F was the modification in fluorescence during excitement. (cancercurehere.com)
  • stimulation
  • We conclude that millicurrent stimulation of human osteoarthritic chondrocytes cultivated in a 3 D collagen gel and of osteochondral explants directly influences cell metabolism. (biomedcentral.com)
  • penicillin
  • Components and strategies Cell lifestyle MCF-7 cells had been grown up in DMEM moderate filled with 10% fetal leg serum and 1% penicillin/streptomycin serum as defined (9). (cancercurehere.com)
  • HAP1 cells are grown in Iscove's Modified Dulbecco's Medium (IMDM) in the presence of 10% fetal calf serum and penicillin/ streptomycin. (horizondiscovery.com)
  • enzyme
  • AK1 is a small ubiquitous enzyme which is essential for maintenance and cell growth. (mybiosource.com)
  • A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. (wikipedia.org)
  • high
  • Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. (wikipedia.org)
  • kinase
  • When the cell is treated with TGF-β, HIPK2, a nuclear kinase, phosphorylates Daxx and the activated Daxx in turn activates the JNK pathway (see "The Daxx Pathway" figure). (wikipedia.org)