• By in situ hybridization expression of the B2t chain was localized to specific epithelial cells in skin, lung, and kidney as opposed to a general epithelial and endothelial cell expression of the laminin B2 chain in the same tissues. (rupress.org)
  • In this process, epithelial cells acquire fibroblast-like properties such as the functional loss of E-cadherin. (spandidos-publications.com)
  • EBV infects cells via the CR2 that was previously thought to be expressed only on the surface of B cells and certain epithelial cells. (nih.gov)
  • pp. 558-572) demonstrate that recombinant human hyaluronan and proteoglycan link protein 1 (rhHAPLN1) significantly reduces the extended airspaces of the emphysematous alveoli by increasing the levels of TGF-β receptor I and SIRT1/6, as a previously unrecognized mechanism in human alveolar epithelial cells, and consequently mitigates COPD. (molcells.org)
  • Furthermore, within intestinal epithelial cells (IECs), miR-146a goals TRAF6, an IL-17R signaling intermediate, to restrict IEC responsiveness to IL-17. (ibs-italy.info)
  • Using microRNA-targeted ZIKV clones engineered to abolish viral infectivity to different parts of the MRS or a library of ZIKV genomes with unique molecular identifiers, we pinpoint epithelial cells of the epididymis (rather than cells of the testis, vas deferens, prostate, or seminal vesicles) as a most likely source of the sexually transmitted ZIKV genomes during the early (most productive) phase of ZIKV shedding into the semen. (bvsalud.org)
  • To examine the involvement of interchromatin granule clusters (IGCs) in transcription and pre-mRNA splicing in mammalian cell nuclei, the serine-arginine (SR) protein kinase cdc2-like kinase (Clk)/STY was used as a tool to manipulate IGC integrity in vivo. (rupress.org)
  • The protein encoded by this gene belongs to the immunoglobulin superfamily and is expressed selectively at the early stages of B cell development, namely, in proB and early preB cells. (nih.gov)
  • The earliest biochemical evidence on the existence of cell-free soluble LH receptor was the purification of an hCG-binding protein, relative molecular mass of 65K (M r , 65K) from porcine follicular fluid and was based on gel filtration followed by affinity chromatography [ 9 ]. (biomedcentral.com)
  • Protein chemistry and cDNA sequencing were used to identify an Aedes albopictus mosquito lysozyme secreted after treatment of cultured cells with heat-killed bacteria. (umn.edu)
  • Examining the genetic code of these resilient cells revealed that Arctic ground squirrels may have a variant form of a protein called ATP5G1. (elifesciences.org)
  • This protein is found in a cellular compartment called the mitochondria, which is responsible for supplying energy to the rest of the cell and therefore plays an important role in metabolic processes. (elifesciences.org)
  • These findings provide the first evidence of a protein variant that is responsible for protecting cells during the metabolic stress conditions caused by hibernation. (elifesciences.org)
  • The protein kinase is found in the cytosol of pea mesophyll cells or in wheat germ extract. (silverchair.com)
  • A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains. (jax.org)
  • The induction of ATF-3 expression was mediated by mitogen-activated protein kinase (MAPK) signaling pathways in GS-002-treated cells. (hindawi.com)
  • Immunoprecipitation experiments with anti-CR2 antibodies and SDS-PAGE analysis reveal a protein with an apparent molecular mass of 155 kDa which is higher than the one seen in B cells. (nih.gov)
  • Paxillin, an integrin-assembly protein, has four major tyrosine phosphorylation sites, and the phosphorylation of Tyr31 and Tyr118 correlates with cell adhesion and migration. (silverchair.com)
  • However, for diagnostic and drug discovery purposes, both of which employ the analysis of a large number of samples, it is attractive to use an outstanding assay system whereby gene expression, protein-protein interaction, ligand binding, and post-transcriptional change can be regulated simultaneously in a one-step response in single-cell extracts. (sg-bio.com)
  • Molecular cloning and transcriptional mapping of the mouse cellular retinoic acid-binding protein gene. (ulaval.ca)
  • We found that Homer3, the predominant isoform in Purkinje cells, is phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) both in vitro and in vivo . (jneurosci.org)
  • In this study, we revealed that Homer3 was phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) in Purkinje cells, and the phosphorylation reduces the affinity for Homer target molecules, resulting in change of the solubility. (jneurosci.org)
  • Protein phosphorylation regulates actomyosin-driven vesicle movement in cell extracts isolated from the green algae, Chara corallina. (bio.net)
  • In 1993, Lee et al cloned a human complementary DNA, rBAT (renal basic amino acid transporter) in chromosome 2, encoding a transport protein for cystine and dibasic amino acids. (medscape.com)
  • cDNA cloning (GenBank Accession Number AF304351), expression of the encoded protein, and functional analysis of cDNA transfected NIH3T3 cells indicated that translation elongation factor-1 delta is a proto-oncogene. (cdc.gov)
  • Hernandez, VP , Higgins, L & Fallon, AM 2003, ' Characterization and cDNA cloning of an immune-induced lysozyme from cultured Aedes albopictus mosquito cells ', Developmental and Comparative Immunology , vol. 27, no. 1, pp. 11-20. (umn.edu)
  • 1. Identification and characterization of Arabidopsis rat mutants and molecular cloning of RAT genes (Gelvin, Citovsky, Hohn, and Ziemienowicz laboratories, with help from undergraduate students at Tuskegee University). (purdue.edu)
  • Cloning and characterization of an active fragment of luciferase from a luminescent marine alga, Pyrocystis lunula. (bio.net)
  • To examine host cell responses to HCV infection, biologists have utilized conventionalhigh throughput (HTS) techniques, such as gene or proteomic expression profiling. (nature.com)
  • This gene encodes the iota polypeptide chain that is associated with the Ig-mu chain to form a molecular complex which is expressed on the surface of pre-B cells. (nih.gov)
  • The complex is thought to regulate Ig gene rearrangements in the early steps of B-cell differentiation. (nih.gov)
  • Mutational Analysis of the VPREB1 Gene of Pre-BCR Complex in a Cohort of Sporadic Pediatric Patients With B-Cell Acute Lymphoblastic Leukemia. (nih.gov)
  • CRISPR/Cas9 mediated knock-out of VPREB1 gene induces a cytotoxic effect in myeloma cells. (nih.gov)
  • PD-1 is a 50-55 kDa cell surface receptor encoded by the Pdcd1 gene that belongs to the CD28 family of the Ig superfamily. (bioxcell.com)
  • The products in this section are designed for molecular cloning of cDNA, gene, PCR fragments (up to 15kb) and also the construction of libraries for much larger fragments (up to 40kb) such as fosmids. (cambio.co.uk)
  • The present invention provides a simple and robust human liver cell-based system in which persistent hepatitis C infection, persistent hepatitis B infection or ethanol exposure induces a clinical Prognostic Liver Signature (PLS) high-risk gene signature. (sumobrain.com)
  • ATF-3 induces DNA synthesis and expression of cyclin D1 in hepatocytes [ 21 ] and is involved in serum-induced cell proliferation as a target gene of c-myc [ 10 ]. (hindawi.com)
  • As a world leader in biotechnology research and development, Takara Bio was the first company to market PCR technology in Japan and is also the developer of the RetroNectin reagent, which is used as a world standard in gene therapy protocols. (takarabio.com)
  • As the sequencing and gene annotation projects of entire genomes of many species are headed towards completion (see, e.g. [13] ), massive mapping efforts in biology are now focused on how the genes interact. (lu.se)
  • The molecular interactions of genes and gene products underlie fundamental questions of biology. (lu.se)
  • Since genes are composed of a given alphabet (A, T, C, G) with fixed pairing properties A-T and C-G, probes can be constructed that attract gene transcripts extracted from cell tissues and cultures. (lu.se)
  • Luciferase is used as a reporter enzyme to estimate gene expression in prokaryotic or eukaryotic cells because the quantity of luciferase correlates with light intensity in the existence of excess luciferin and ATP. (sg-bio.com)
  • to acheive this aim the stomoxyn-zy gene was amplified by rapid amplification of cDNA ends (RACE) from Hermetia illucens L larvae stimulated with S. aureus , and cloned into the pPICZαA vector. (ukessays.com)
  • To identify human PPARs-responsive genes, we established tetracycline-regulated human hepatoblastoma cell lines that can be induced to express each human PPAR and investigated the gene expression profiles of these cells. (biomedcentral.com)
  • The FA2 gene of Chlamydomonas encodes a NIMA family kinase with roles in cell cycle progression and microtubule severing during deflagellation. (bio.net)
  • Differential display analysis of gene expression revealed the overexpression of translation elongation factor-1 delta sub-unit in BALB/c-3T3 cells transformed with cadmium chloride compared with the non- transformed control cells. (cdc.gov)
  • In combination with transgenic organisms, such enzyme-actuated antisense tools will enable gene silencing in specific cell types, including tissues that are not amenable to optical targeting. (cdc.gov)
  • 11 , 12 Light-dependent gene silencing can be achieved through whole-organism irradiation or the targeted illumination of specific cell populations. (cdc.gov)
  • Here, we report a method that combines transcriptome expression analysiswith unbiased proteome reactivity profiling to identify novel host cell responsefactors in HCV infection. (nature.com)
  • Induced PD-L1 expression is common in many tumors including squamous cell carcinoma, colon adenocarcinoma, and breast adenocarcinoma. (bioxcell.com)
  • Additionally, the truncated Lhcgr, misrouted the full-length Lhcgr in ER, thereby decreasing the cell surface expression of the mature functional receptor. (biomedcentral.com)
  • instead, a specific intra-cellular role of truncated receptor in regulating the cell surface expression of a full-length ligand-binding Lhcgr has been proposed [ 15 ]. (biomedcentral.com)
  • To address this issue, the expression levels of Snail, RhoA and fibronectin, as well as MMP‑2, were reduced in the breast tumor cell lines MDA‑MB‑231 and MDA‑MB‑435S, and their biological responses were studied in vitro and in vivo. (spandidos-publications.com)
  • Through cell survival-based cDNA expression screens in neural progenitor cells, we identify a genetic variant of AGS Atp5g1 that confers cell resilience to metabolic stress. (elifesciences.org)
  • Ectopic expression in mouse cells and CRISPR/Cas9 base editing of endogenous AGS loci revealed causal roles of one AGS-specific amino acid substitution in mediating cytoprotection by AGS ATP5G1. (elifesciences.org)
  • The activation of exogenous AhR in N2a-Rα cells was confirmed using RNAi, with si-AhR suppressing the expression of exogenous AhR. (biomedcentral.com)
  • Morphologically, N2a-Rα cells bore spontaneous neurites exhibiting axon-like properties with the localization of NF-H. In addition, cdc42 expression was increased in comparison to the control cell line. (biomedcentral.com)
  • SCARA5 expression is significantly downregulated in various tumor tissues including liver cancer (LC), rectal cancer, and glioblastoma [ 15 , 16 ] and might affect cell proliferation and invasion abilities by the focal adhesion kinase (FAK) signaling pathway and epithelial-mesenchymal transition (EMT) [ 5 , 17 - 20 ]. (hindawi.com)
  • These phenotypes were similar to those induced by RhoA(G14V) in parental cells, and could be abolished by expression of RhoA(T19N), Rac1(G12V), or p190RhoGAP in the mutant-expressing cells. (silverchair.com)
  • We show here that expression of EBF3 resulted in cell cycle arrest and apoptosis. (aacrjournals.org)
  • The expression of cyclin-dependent kinase inhibitors was profoundly affected with early activation and then repression of p21 cip1/waf1 and persistent activation of both p27 kip1 and p57 kip2 , whereas genes involved in cell survival and proliferation were suppressed. (aacrjournals.org)
  • ALiCE® is a radically different approach to cell-free expression and a kit unlike any others. (amsbio.com)
  • Nowadays, molecular biology researchs are featured a wide range of antimicrobial peptides (AMP)-related topics such as AMP genome library construction, development of antimicrobial peptides and their mimetics as therapeutic agents, structure/function analysis, cloning and expression, regulation, mechanisms of action, microbial escaping strategies and human disease associations. (ukessays.com)
  • We found that the expression of each PPAR subtype was tightly controlled by the concentration of doxycycline in these established cell lines. (biomedcentral.com)
  • Clinical significance of L1CAM expression and its biological role in the progression of oral squamous cell carcinoma. (nih.gov)
  • Expression of the antisense translation elongation factor-l delta mRNA resulted in marked reversal of the oncogenic potential of the cadmium -transformed BALB/c- 3T3 cells. (cdc.gov)
  • Thrombospondin type 1 domain-containing 7A ( THSD7A ) was reported to play a procancer role in esophageal squamous cell carcinoma (ESCC). (hindawi.com)
  • Therefore, it is important to explore candidate genes that could have significance to the clinical diagnosis, as well as the treatment of esophageal squamous cell carcinoma (ESCC) at the molecular level [ 4 ]. (hindawi.com)
  • Mechanochemical Principles of Spatial and Temporal Patterns in Cells and Tissues. (mpi-cbg.de)
  • Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
  • C. Peterson, M. Ringne´r / Artificial Intelligence in Medicine 28 (2003) 59-74 structure and function, cellular metabolism, development of cells and tissues, and response of organisms to their environments. (lu.se)
  • Among the possible luciferase-luciferin reactions, the firefly luciferase and firefly D-luciferin pair is utilized most widely, because the luminescence produced by the reaction can be quantified specifically, and has an incredibly low background, and because firefly D-luciferin is highly stable and easily permeates cells and tissues. (sg-bio.com)
  • DNA microarray analyses using these cell lines were performed with or without adding each subtype ligand and provided much important information on the PPAR target genes involved in lipid metabolism, transport, storage and other activities. (biomedcentral.com)
  • diagnostic prediction of small round blue cell tumors (SRBCT) of childhood and determining the estrogen receptor (ER) status of sporadic breast cancer. (lu.se)
  • Interestingly, the levels of some soluble cell surface proteins including sE-cadherin that are known to promote metastasis are also dramatically reduced in MLKL null tumors/mice. (biomedcentral.com)
  • Administration of ADAMs pan inhibitor reduces the levels of soluble cell surface proteins in WT tumors/mice and leads to the dramatic decrease in metastasis. (biomedcentral.com)
  • Furthermore, sub-cutaneous injection of the antisense mRNA expressing transformed BALB/c-3T3 cells resulted in a significant reduction in the onset as well as the size of tumors in athymic nude mice. (cdc.gov)
  • A second set of clones contained an alternative 3' end sequence giving a total of 4,316 bp. (rupress.org)
  • Single-cell sequencing has made considerable progress relying on the notion that the sequence is the tag. (nature.com)
  • The full length cDNA sequence was used to deduce the sequences and masses of theoretical tryptic peptides that would be detected after matrix-assisted laser desorption ionization time of flight (MALDI-TOF) and tandem mass spectrometry (MS/MS). In aggregate, this analysis uncovered seven peptides that encoded 75 of the 125 amino acids in the mature Ae. (umn.edu)
  • One of the clones possesses an identical nucleotide sequence to the one corresponding to B cell CR2, whereas the other represents a differentially spliced transcript which lacks the exon 8b of B cell CR2. (nih.gov)
  • We present a method enabling simultaneous, ultra-high throughput single-cell barcoding of millions of cells for targeted analysis of proteins and RNAs. (nature.com)
  • We demonstrate that recombinant LHCGR proteins which include the hormone-binding domain are secreted from transfected cells and that natural LHCGR is also secreted from human placental explants. (biomedcentral.com)
  • LHCGR recombinant proteins representing varying lengths of the N-terminal extracellular domain were expressed in Chinese Hamster Ovary cells in suspension culture. (biomedcentral.com)
  • These proteins control the dynamics of the actin cytoskeleton and thus represent key regulatory molecules that are active during cell migration ( 12 ). (spandidos-publications.com)
  • Proteins were transferred onto a nitrocellulose membrane and immunoblotting was performed with mouse monoclonal anti-RIPK2 (clone 6F7, Sigma-Aldrich, Cat#SAB1404621, dilution 1? (ibs-italy.info)
  • MLKL activation also leads to the protease, ADAMs-mediated ectodomain shedding of cell surface proteins of necroptotic cells. (biomedcentral.com)
  • Here, we report that the ectodomain shedding of cell surface proteins of necroptotic cells is critical for the promoting effect of tumor necroptosis in tumor metastasis through inhibiting the anti-tumor activity of T cells. (biomedcentral.com)
  • We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells. (amsbio.com)
  • When cDNAs for these luciferases are transfected into mammalian cells, the expressed luciferases are emitted into the medium via the endoplasmic reticulum and the Golgi complex. (sg-bio.com)
  • Metronidazole (Flagyl) is a drug used to treat infections and has been studied in the treatment of cancer [10,11] as a radiosensitizer drug, especially for hypoxic cells [9]. (who.int)
  • tizer drug, especially for hypoxic cells [ 9 ]. (who.int)
  • Overlapping clones were isolated from a human fibrosarcoma HT1080 cell cDNA library spanning a total of 5,200 bp. (rupress.org)
  • Human peripheral blood mononuclear cells (PBMC) were stained with the PE-Cy™7 Mouse Anti-Human CD194 antibody in conjunction with either a FITC Mouse Anti-Human CD4 antibody (Cat. (bdbiosciences.com)
  • In this study, we examined the antitumor effects of GS-002 in human hepatoma Hep3B and HepG2 cells in vitro . (hindawi.com)
  • Our study shows that Jurkat human T cells have a molecule that reacts with both anti-CR2 antibodies and the third component of complement, C3. (nih.gov)
  • The results suggest that these cell lines are important tools used to identify the human PPARs-responsive genes. (biomedcentral.com)
  • In vitro studies revealed similar cell permissiveness and replication of CSUR381 (compared with 2 other NiV isolates) in both bat and human cell lines. (cdc.gov)
  • The approach was applied to five different hybridomas producing human monoclonal antibodies and variable regions for both bold gamma and mu heavy chain and kappa and lambda light chain genes were successfully cloned. (lu.se)
  • These results demonstrate that the cell transformation and tumorigenesis induced by the human carcinogen - cadmium , were due to the overexpression of translation elongation factor-1 delta - a novel cadmium -responsive proto-oncogene. (cdc.gov)
  • Currently, with the application of molecular biology resources in human identification, it became possible to identify a person even without physical ante-mortem data or with deteriorated biological material in negligible amounts, which are actually relatively common conditions in forensic analyses 7,12,16,19 . (bvsalud.org)
  • Cell-based assay systems using reporter enzymes are utilized widely to review promoters, interactions between promoters and transcription factors, signal transduction, and other cellular activities. (sg-bio.com)
  • We envisioned that enzyme-activatable cMOs could overcome these limitations, as the triggering enzymes could be expressed in individual cell populations using cis regulatory elements. (cdc.gov)
  • cDNA cloning of mutant catalase in acatalasemic beagle dog: Single nucleotide substitution leading to thermal-instability and enhanced proteolysis of mutant enzyme. (wisdompanel.com)
  • During the early 1990s, breakthroughs in cloning and sequencing of Norwalk virus and Southampton virus ( 15--18 ) led to the development of sensitive molecular assays (e.g., reverse transcription-polymerase chain reaction [RT-PCR]), nucleotide hybridization probes, and enzyme-linked immunosorbent assays (ELISA) that used baculovirus-expressed viral antigens ( 19--33 ). (cdc.gov)
  • Cell-based assays are also involved in drug screening both in vitro and in vivo. (sg-bio.com)
  • One benefit of using a luciferase reporter is that the luminescence can be calculated quantitatively and longitudinally with real-time resolution in vitro and in vivo when a bioluminescent substrate is conducted on cells. (sg-bio.com)
  • It is the in vivo situation where blood cells are worth mentioning that many of the electron- suspended in their original medium. (who.int)
  • The hormonal activation occurs through specific ligand-receptor interactions on the surface of the target cells. (biomedcentral.com)
  • A different experimental approach by West and Cooke [ 10 ] demonstrated the secretion of M r 80-90K hCG-LH receptor complex into the culture media from ligand-induced rat and mouse leydig cells. (biomedcentral.com)
  • 11 ] first reported the production and secretion of soluble LH receptor following transient transfection of a naturally truncated variant of the receptor in COS cells. (biomedcentral.com)
  • Recent findings in our laboratory and those of others, however, have shown that the EBV receptor is also present on T cells. (nih.gov)
  • This study investigated the link between GM and the intrahepatic T cell receptor (TCR) immune repertoire (IR), and whether GM modulates the intrahepatic immune microenvironment via TCR IR during liver fibrosis. (frontiersin.org)
  • Intestinal antigens (IAg) (originating either from pathogenic microorganisms or from food) that enter the portal circulation, are captured by intrahepatic antigen-presenting cells (APCs), leading to T cell receptor (TCR) recognition and hence the activation of the adaptive immune system. (frontiersin.org)
  • Finally, we showed the sE-cadherin/KLRG1 inhibitory receptor is the major pathway for necroptosis-mediated suppression of the anti-tumor activity of T cells and the promotion of metastasis. (biomedcentral.com)
  • Furthermore, both robust phosphorylation of Homer3 and its dissociation from metabotropic glutamate receptor 1α (mGluR1α) were triggered by depolarization in primary cultured Purkinje cells, and these events were inhibited by CaMKII inhibitor. (jneurosci.org)
  • With minimal instrumentation (four 96-well plates and a multichannel pipette), cell-specific codes are added to each tagged molecule within cells through sequential rounds of classical split-pool synthesis. (nature.com)
  • However, both the accumulation of splicing factors at sites of pre-mRNA synthesis as well as pre-mRNA splicing are dramatically reduced, demonstrating that IGC disassembly perturbs coordination between transcription and pre-mRNA splicing in mammalian cell nuclei. (rupress.org)
  • Single-cell omics provide insight into cellular heterogeneity and function. (nature.com)
  • Although hypoxic or ischemia tolerance in AGS involves physiological adaptations, little is known about the critical cellular mechanisms underlying intrinsic AGS cell resilience to metabolic stress. (elifesciences.org)
  • As a necrotic cell death, the rupture of cell plasma membrane of necroptotic cells results in the release of many cellular factors of necroptotic cells, which may trigger inflammation and immune responses [ 17 , 18 ]. (biomedcentral.com)
  • Thus, exogenous AhR induced catecholaminergic differentiation in N2a-Rα cells. (biomedcentral.com)
  • The excessive activation of AhR resulted in neural differentiation of Neuro2a cells. (biomedcentral.com)
  • Activated AhR may disrupt the strictly regulated brain formation with irregular differentiation occurring rather than cell death. (biomedcentral.com)
  • This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. (nih.gov)
  • The goals for advances in flow cytometry are clear: measure as many relevant target molecules per cell as quickly as possible. (nature.com)
  • PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. (bioxcell.com)
  • Additionally, Pdcd1 mRNA is expressed in developing B lymphocytes during the pro-B-cell stage. (bioxcell.com)
  • The cells were gated on lymphocytes and quadrant markers for the bivariate dot plots were set based on staining with a PE-Cy™7 Mouse IgG1 κ Isotype Control (MOPC-21). (bdbiosciences.com)
  • The development of skin sensitization is associated with, and requires, the activation and clonal expansion of allergen responsive T lymphocytes and it is these cells that orchestrate the cutaneous allergic reaction. (cdc.gov)
  • This approach will permit analysis of B cell clonal ontogeny, antibody diversity and lymphoma cell progression and heterogeneity. (lu.se)
  • Both of these latter techniques risk changes to the metabolism during cell preparation and barcoding prior to cell lysis- since both approaches, generally, rely on the use of live cells 30 . (nature.com)
  • PD-L1 overexpression results in increased resistance of tumor cells to CD8 T cell mediated lysis. (bioxcell.com)
  • Improvements in the transient transfection assay system have added luciferase as an internal control reporter (dual-reporter assay), thereby minimizing inherent experimental variability that can undermine experimental accurateness, such as variations in the number and viability of cells utilized, the efficiency of cell transfection and lysis, and so on. (sg-bio.com)
  • Silencing of the EBF3 locus was observed in brain, colorectal, breast, liver, and bone tumor cell lines and its reactivation was achieved on treatment with 5-aza-2′-deoxycytidine and trichostatin A in a significant portion of these tumor cells. (aacrjournals.org)
  • Affordable & consistent controls from 50 tumor cell lines, ideal for IHC, in situ hybridization & NGS applications. (amsbio.com)
  • On thebasis of this result, we screened the proteome reactivity using chemical probescontaining thiol-reactive functional groups and discovered a unique labeling profilein HCV-infected cells. (nature.com)
  • N2a-Rα cells exhibited two significant functional features. (biomedcentral.com)
  • N2a-Rα cells expressed tyrosine hydroxylase (TH) mRNA as a functional marker of catecholaminergic neurotransmitter production. (biomedcentral.com)
  • Information obtained from the US federal contracts registry clarifies some of the military activities at The Lugar Center - among them research on bio-agents (anthrax, tularemia) and viral diseases (e.g. (dilyana.bg)
  • During C4 photosynthesis, an evolved pathway to increase localized CO2 concentrations under the threat of enhanced photorespiration, CO2 is captured within mesophyll cells, fixed as oxaloacetate, converted into malate and released internally within bundle sheath cells to directly feed RuBisCO activity. (wikipedia.org)
  • Many desirable features, such as low cost of production, rapidity, heat-tolerant, relatively broad antimicrobial spectrum and low toxicity to eukaryotic cells made of antimicrobial peptides to be a new alternative to the conventional antibiotic [2]. (ukessays.com)
  • Thank you for your interest in our Luciferase Tagging Cell Line Construction Service services. (sg-bio.com)
  • Metronidazole selectively radiosensitizes hypoxic cells without influencing the radiation response of normal well-oxygenated cells [5,7-9]. (who.int)
  • Metronidazole selectively radiosensitizes of metronidazole (in a range of therapeutic hypoxic cells without influencing the ra- concentrations) on PMN-generated free diation response of normal well-oxygenated radicals during phagocytosis activity in cells [ 5,7-9 ]. (who.int)
  • We found that mutation of Tyr31/118 caused enhanced activation of RhoA and premature formation of stress fibers with substantial loss of efficient membrane spreading and ruffling in adhesion and migration of NMuMG cells. (silverchair.com)
  • We conclude that Tyr31/118-phosphorylated paxillin serves as a template for the localized suppression of RhoA activity and is necessary for efficient membrane spreading and ruffling in adhesion and migration of NMuMG cells. (silverchair.com)
  • The interaction between the GM and intrahepatic immune microenvironment is regulated and stabilized by a complex network of factors including IAg, immune cells, and cytokines. (frontiersin.org)
  • to limit myeloid cell-derived IL-17-inducing cytokines and restrict colonic IL-17. (ibs-italy.info)
  • In recent years, much has been learned of the characteristics of immune responses to skin sensitizing chemicals and of the roles played by dendritic cells, cytokines and chemokines. (cdc.gov)
  • and regulation of cell cycle process. (nih.gov)
  • The present data provide evidence that Snail promotes tumor cell motility and angiogenesis which is mainly mediated through the regulation of RhoA activity. (spandidos-publications.com)
  • Secreted luciferases, CLuc, MetLuc, and GLuc, have been cloned from bright organisms such as the marine ostracod Cypridina noctiluca, and marine copepods Metridia longa and Gaussia princeps. (sg-bio.com)
  • Both immunofluorescence and transmission electron microscopy analyses of cells overexpressing Clk/STY indicate that IGC components are completely redistributed to a diffuse nuclear localization, leaving no residual structure. (rupress.org)
  • found that when this variant form of ATP5G1 was introduced into the cells of mice, their mitochondria was better at coping with stress conditions, such as low oxygen, low temperature and poisoning. (elifesciences.org)
  • Sorting in plant cells is complex, as mitochondria and chloroplasts or plastids coexist in every cell. (silverchair.com)
  • Commercial approaches have costs ranging from $1 to $5 per cell per expressome. (nature.com)
  • albopictus lysozyme cDNA was cloned using PCR-based approaches. (umn.edu)
  • This size is consistent with that of B cell CR2 mRNA. (nih.gov)
  • Other recent studies verified the presence of PAPP-A mRNA in granulosa cells of humans, monkeys, cattle, mice, and pigs. (bioone.org)
  • Increases in the amount of PAPP-A mRNA in granulosa cells during follicular development occurs in some but not all species, indicating that other proteases or protease inhibitors may be involved in IGFBP degradation. (bioone.org)
  • In order to confirm the oncogenic potential of translation elongation factor-l delta, BALB/c-3T3 cells transformed with cadmium chloride were transfected with the plasmid DNA expressing antisense TEF-I delta mRNA. (cdc.gov)
  • This was evidenced by a significant reduction in the number of anchor- age independent colonies developing from the transformed cells expressing antisense mRNA for translation elongation factor-1 delta compared with the vector alone-transfected control cells. (cdc.gov)
  • RhoA has been implicated in the formation of stress fibers and cell adhesion in fibroblasts. (spandidos-publications.com)
  • We found that Tyr31/118-phosphorylated paxillin competes with p190RhoGAP for binding to p120RasGAP, and provides evidence that p190RhoGAP freed from p120RasGAP efficiently suppresses RhoA activity during cell adhesion. (silverchair.com)
  • Interestingly, cells without intact IGCs continue to synthesize nascent transcripts. (rupress.org)
  • In addition, EBV variably infects Jurkat cells as demonstrated by the presence of transcripts of Epstein Barr nuclear Ag (EBNA-1) using the polymerase chain reaction. (nih.gov)
  • NfsB is a dimeric flavoprotein enzyme that has broad electron acceptor specificity 15 and has been used in conjunction with prodrugs to selectively ablate cells in vertebrates. (cdc.gov)
  • therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination. (bdbiosciences.com)
  • Takara Bio USA, Inc. (formerly Clontech Laboratories, Inc.), a wholly owned subsidiary of Takara Bio Inc., develops, manufactures, and distributes a wide range of life science research reagents under the Takara, Clontech, and Cellartis brands. (takarabio.com)
  • We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)
  • Recent technological advances have accelerated single-cell analyses, but workflows remain expensive and complex. (nature.com)
  • RAW 264.7 and CMT-93 cells were grown in Dulbeccos modified Eagles medium supplemented with 10% FBS and were co-transfected with psiCHECK-2 vector containing 3-UTR variants and either control or miR-146a mimic oligos. (ibs-italy.info)
  • Together, these findings suggested that Homer3 in Purkinje cells might function as a reversible coupler regulated by CaMKII phosphorylation and that the phosphorylation is capable of regulating the postsynaptic molecular architecture in response to synaptic activity. (jneurosci.org)
  • These results suggest that the phosphorylation may regulate postsynaptic molecular architecture and Ca 2+ signaling of Purkinje cells in response to synaptic activity. (jneurosci.org)
  • Mountain View, CA-April 26, 2016- Takara Bio USA, Inc. (TBUSA, formerly Clontech Laboratories, Inc.), a wholly owned subsidiary of Takara Bio Inc., and Integrated DNA Technologies (IDT) today announced a new collaboration to support targeted RNA sequencing. (takarabio.com)
  • The in vitro effects of metronidazole on the production of reactive oxygen species by polymorphonuclear (PMN) cells were studied by means of nitroblue tetrazolium and luminol-dependent chemiluminescence. (who.int)
  • Mass spectral analysis of the silver-stained band uncovered five tryptic peptides with masses that matched peptides from an Aedes aegypti lysozyme, which we had previously characterized from the Aag-2 mosquito cell line. (umn.edu)
  • Northern blot analysis of Jurkat poly(A)+ RNA shows a transcript of 4.7 kb upon probing with the B cell CR2 cDNA. (nih.gov)
  • Battelle has operated a Top Secret Bio laboratory (National Biodefense Analysis and Countermeasures Center - NBACC) at Fort Detrick, Maryland under a US Department of Homeland Security (DHS ) contract for the last decade. (dilyana.bg)
  • From transfection or fusion, expansion, screening, clone selection and titer assays, the Stable Cell Line Services offered by Seattle Genova are prepared to meet all of your custom cell line requirements. (sg-bio.com)