• cDNA microarrays are capable of profiling gene expression patterns of tens of thousands of genes in a single experiment. (nih.gov)
  • This combination of reverse transcription and PCR (RT-PCR) allows the detection of low abundance RNAs in a sample, and production of the corresponding cDNA, thereby facilitating the cloning of low copy genes. (neb.com)
  • Cloning a cDNA library leads to over-representation of clones of highly expressed genes, whereas "least abundant" mRNAs can only be examined with difficulty. (bio-medicine.org)
  • The amplified cDNA populations under comparison are then subjected to suppression subtractive hybridization (SSH-PCR), a technique that couples subtractive hybridization with suppression PCR to selectively amplify fragments of differentially expressed genes. (springer.com)
  • Each Marathon-Ready cDNA is a premade, tissue-specific "pool" of double-stranded cDNA from which full-length genes can be amplified by using sets of gene-specific primers. (clontech.com)
  • cDNA is produced from fully transcribed mRNA found in the nucleus and therefore contains only the expressed genes of an organism. (wikipedia.org)
  • It has been specially formulated for the amplification of full-length cDNAs representing the majority of human genes. (cellartis.com)
  • JCVI: Receptor Cloning: High Throughput Sequencing of CDNA Tags for Identification of Novel Genes. (jcvi.org)
  • Background: Along with the draft sequencing of the pig genome, which has been completed by an international consortium, collection of the nucleotide sequences of genes expressed in various tissues and determination of entire cDNA sequences are necessary for investigations of gene function. (ebscohost.com)
  • these 'tags' enable the reading of cDNA, lengths of copied genetic material identical to genes. (sciencephoto.com)
  • Este cDNA foi sintetizado por meio de RT-PCR utilizando-se mRNA extraído de tubérculo de batata (Solanum tuberosum L.). Os primers específicos para a ORF do gene da UGPase foram definidos com base em seqüências conservadas entre genes ortólogos, já disponíveis em bancos de dados. (usp.br)
  • As a result of genomics initiatives worldwide, it has become increasingly easy to obtain cDNA clones representing the 3 ends of many human genes. (currentprotocols.com)
  • A high-throughput direct-differential cDNA sequencing approach was employed to identify genes differentially expressed in normal breast as compared with breast cancer. (aacrjournals.org)
  • Of many putative differentially expressed genes, a breast cancer-specific gene, BCSG1 , which was expressed in high abundance in a breast cancer cDNA library but scarcely in a normal breast cDNA library, was identified as a putative breast cancer marker. (aacrjournals.org)
  • To determine the feasibility of using gene expression as an indicator of chemical contamination, cDNA microarrays containing D. magna genes related to toxicant stress have been constructed. (epa.gov)
  • The Communicable Diseases Network Australia (CDNA) was established in 1989 as the Communicable Diseases Control Network, as a joint initiative of the National Health and Medical Research Council and Australian Health Ministers' Advisory Council. (health.gov.au)
  • The membership of the Communicable Diseases Network Australia ('CDNA') and the Commonwealth of Australia ('the Commonwealth'), as represented by the Department of Health (Health), does not warrant or assume any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, or process disclosed at the time of viewing by interested parties. (health.gov.au)
  • However, no study has thus far addressed the consequences of INI treatment on HIV-1 cDNA species on any cell population in vivo. (clinicaltrials.gov)
  • An accumulation of circular episomal HIV-1 cDNA species may also be a consequence of RGV treatment in this cell type. (clinicaltrials.gov)
  • To explore raltegravir-induced shifts in HIV-1 cDNA species in vivo, this non-interventional clinical observation investigates the dynamics of the three major HIV-1 cDNA species (total HIV-1 cDNA, HIV-1 integrants in the host cell genome, episomal HIV-1 2-LTR circles) over a period of 4 months in two groups of patients starting off cART from a single study center. (clinicaltrials.gov)
  • BioCat is pleased to offer a wide variety of highest quality oligo dT-primed first strand cDNA from many different species, tissues, and diseases. (biocat.com)
  • Preliminary analyses of PBMCs from HIV-infected patients indicate that all three major HIV-1 cDNA species can be quantified by real-time PCR under these baseline conditions. (clinicaltrials.gov)
  • LSBio offers a selection of over 13,000 cDNA clones in cloning vectors covering a variety of research species. (lsbio.com)
  • If the cDNA was made using oligodT primers, for instance, it is simply a matter of the reverse transcriptase making it further 5' in some of the clones. (protocol-online.org)
  • Still, if the cDNA was made with random primers, you may still get differnt lengths. (protocol-online.org)
  • after the RT you assume that you have 2µg cDNA (if you used random primers) that means, you have 100ng / µl cDNA. (protocol-online.org)
  • The complete coding sequence of the rabbit galectin-3-encoding cDNA (LGALS3) has been cloned in a single step by using RT-PCR and specific human LGALS3 cDNA primers. (archives-ouvertes.fr)
  • We spliced some fragments to a whole mouse lrg -cDNA sequence and designed a pair of primers at completely homologous fragments in 5'-UTR and 3'-UTR, we amplified mouse lrg -cDNA by RT-PCR. (scirp.org)
  • This highly customisable sequence shows, by default, the transcript sequence (cDNA), the coding sequence underneath it, and the protein sequence in the third line. (vectorbase.org)
  • The cDNAs of novel identified Open Reading Frames(ORF) are tagged with Green Fluorescent Protein (GFP) and expressed in eukaryotic cells. (wikipedia.org)
  • As the sequence of the cDNAs is known, bioinformatics can make predictions regarding the localisation and function of the encoded protein. (wikipedia.org)
  • Background: The German cDNA Consortium has been cloning full length cDNAs and continued with their exploitation in protein localization experiments and cellular assays. (ebscohost.com)
  • Above all, it is intriguing that the original cDNA-RDA fragment has now been identified as the novel hypothetical protein LOC310133 in chromosome 2. (bl.uk)
  • Then sticky ends are produced in the cDNA molecule by cleaving the cDNA ends (which now have linkers with an incorporated site) with the appropriate endonuclease. (wikipedia.org)
  • That cDNA as a molecule does not exist in nature is irrelevant in circumstances where the Court has formed the view that the relevant claim is for information that does exist in nature, and that information "also attaches to cDNA" . (lexology.com)
  • That being the case, there are good arguments - based on the artificiality of cDNA - that a claim that is properly construed as defining a molecule - and not information - should be held valid on the patentable subject matter ground. (lexology.com)
  • If you are interested in learning more or discussing how cDNAs, ORFs or CRISPRa might be useful in your experiment, please just email or call our Tech Support team! (horizondiscovery.com)
  • also enriched for rare cDNAs. (flybase.org)
  • This process leads to a decrease in the number of frequently occurring cDNAs, and an increase in rare cDNAs. (bio-medicine.org)
  • Due to the likelihood of the two matching single strands of a frequently-occurring cDNA coming together in this mixture of molecules, the frequently-occurring cDNAs reassociate much more quickly than rare cDNAs. (bio-medicine.org)
  • In the screen for DON-resistance conferring wheat cDNAs we found 18 plasmids containing 8 different types of inserts. (europa.eu)
  • To generate amounts of nucleic acids sufficient for use in standard cloning strategies, we have devised a method for producing amplified heterogeneous populations of RNA from limited quantities of cDNA. (pnas.org)
  • Having arrived at this point, it is perhaps not surprising that the majority was then able to group cDNA with other nucleic acids within the claim as patent ineligible subject matter. (lexology.com)
  • cDNA was synthesized using an oligo(dT) primer and purified to remove interfering RNA. (cellartis.com)
  • The cDNA sequence contains a 2257-amino acid reading frame encoding a 251-kDa polypeptide. (pnas.org)
  • The B. mori beta-glucosidase cDNA was expressed as a 57-kDa polypeptide in baculovirus-infected insect Sf9 cells, and the recombinant beta-glucosidase was active on cellobiose and lactose, but not active on salicin, indicating that the B. mori beta-glucosidase possesses the characteristics of the Class 2 enzyme. (unboundmedicine.com)
  • DNA targets, in the form of 3' expressed sequence tags (ESTs), are arrayed onto glass slides (or membranes) and probed with fluorescent- or radioactively-labelled cDNAs. (nih.gov)