• Here, we show that in the mouse striatum, dopamine receptor stimulation negatively regulates phosphorylation of Tyr15-Cdk5 most likely through a D2R-mediated mechanism. (frontiersin.org)
  • These findings have important implications for understanding the mechanisms by which the Cdk5/DAPP-32-Thr75 signaling regulates the striatal functions. (frontiersin.org)
  • Neurons will send out both dendrites and axons to form connections with other neurons in order to transmit information, and Cdk5 regulates this process. (wikipedia.org)
  • It is also worth noting that Cdk5 also regulates the process of apoptosis, which is necessary in order to assure that the neural connections that are formed are correct. (wikipedia.org)
  • On top of that, Cdk5 modulates actin-cytoskeleton dynamics by phosphorylating Pak1 and filamin 1 and regulates the microtubules by also phosphorylating tau, MAP1B, doublecortin, Nudel, and CRMPs, which are all microtubule-associated proteins. (wikipedia.org)
  • The Cdk5 activator P39 specifically links muskelin to myosin II and regulates stress fiber formation and actin organization in lens. (nih.gov)
  • CDK5 phosphorylates eNOS at Ser-113 and regulates NO production. (drugbank.com)
  • regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. (proteopedia.org)
  • CTNNB1 phosphorylation regulates insulin internalization. (proteopedia.org)
  • On the mechanistic level, we show that Cdk5 phosphorylates the forkhead transcription factor Foxc2 which regulates Foxc2-dependent transcription. (oncotarget.com)
  • Recently, it has been shown that phosphorylation regulates Foxc2-mediated transcription in lymphatic endothelial cells [ 11 ]. (oncotarget.com)
  • By acting as a ligand-dependent transcription factor of the glucocorticoid receptor (GR) mediates the action of glucocorticoids and regulates many physiological processes. (pp1a.com)
  • Here, we show that cyclindependent kinase 5 (Cdk5), a protein kinase that regulates cell migration and invasion, phosphorylates PIPKIγ90 at S453, and that Cdk5-mediated PIPKIγ90 phosphorylation is essential for cell invasion. (arizona.edu)
  • Moreover, Cdk5-mediated phosphorylation down-regulates the activity of PIPKIγ90 and the secretion of fibronectin, an extracellular matrix protein that regulates cell migration and invasion. (arizona.edu)
  • These data suggest that Cdk5-mediated PIPKIγ90 phosphorylation regulates cell invasion by controlling PIPKIγ90 activity and fibronectin secretion. (arizona.edu)
  • Cdk5 is abundant and mainly expressed in neurons, where it phosphorylates protein polymers with a high molecular weight called neurofilaments, and microtubule-associated protein tau, which are abundant in the CNS (Central Nervous System). (wikipedia.org)
  • The main role of Cdk5 when it comes to neurons is to assure proper neuronal migration. (wikipedia.org)
  • Analyses of endogenous Kv2.2 in cortical neurons in situ and recombinant Kv2.2 expressed in heterologous cells reveal that Kv2.2 is largely refractory to stimuli that trigger robust, phosphorylation-dependent changes in Kv2.1 clustering and function. (jneurosci.org)
  • We find that Kv2.1 and Kv2.2 exhibit distinct responses to acute phosphorylation-dependent regulation in brain neurons in situ and in heterologous cells in vitro . (jneurosci.org)
  • Besides sharing both up- and downstream pathways, Cdk5 and GSK3β are linked more directly in neurons. (frontiersin.org)
  • using cultured neurons: Their investigations to determine a regulatory pathway for kinesin-driven motility in axons revealed that Cdk5 is neccessary for kinesin-driven motility within the axon but cannot directly phosphorylate kinesin. (frontiersin.org)
  • Recent studies provide important in vivo evidence that hyperactivation of Cdk5 by p25 plays an essential role in the cell death of neurons in experimental models of AD and PD. (drugbank.com)
  • These results indicated that Cdk5/p35 in excitatory neurons is important for the hippocampal synaptic plasticity and associative memory retention. (biomedcentral.com)
  • Using behavioral and electrophysiological analyses, we investigate whether the p35/Cdk5 activity is involved in associative memory learning in excitatory or inhibitory neurons. (biomedcentral.com)
  • Unlike other cyclin-dependent kinases, it is expressed predominantly in post-mitotic neurons, its activity modulated by association with nervous system-specific propeins. (pathologyresjournal.com)
  • Here, we investigated the mechanism by which cyclin I safeguards against apoptosis and found that cyclin I bound and activated cyclin-dependent kinase 5 (Cdk5) in isolated mouse podocytes and neurons. (jci.org)
  • Inhibition of Cdk5 by olomoucine enhanced phosphorylation of kinesin, suggesting that the effect was mediated by negative regulation of other kinases. (frontiersin.org)
  • Inhibition of Cdk5 resulted in dephosphorylation of the inhibitory Ser9 site on GSK3β and an increase of activating phosphorylation at Tyr216. (frontiersin.org)
  • Displays sub-micromolar inhibition of cdk1, cdk2, cdk5, cdk7 and cdk9 (reported IC 50 values range from 0.16 - 0.28 μM for cdk5 to 0.8 μM for cdk7). (tocris.com)
  • Furthermore, inhibition of PIPKIg activity with the chemical inhibitor UNC3230 suppresses fibronectin secretion in a dose-dependent manner, whereas depletion of Cdk5 enhances fibronectin secretion. (arizona.edu)
  • Cdk5 activity was reduced in glomeruli and brain lysates from cyclin I-deficient mice, and inhibition of Cdk5 increased in vitro the susceptibility to apoptosis in response to cellular damage. (jci.org)
  • While cyclin-dependent kinase 5 (Cdk5) is of growing importance to neuronal signaling, its regulation remains relatively unexplored. (elsevierpure.com)
  • Although no evidence for the involvement of the Cdk5 cofactors p35 or p39, or for L- and T-type voltage-gated Ca 2+ channels, was found, evaluation of the role of phosphatases and extracellular cations revealed differential regulation of the three sites. (elsevierpure.com)
  • Thus, in the striatum the regulation of phosphorylation of Cdk5-dependent sites by NMDA occurs through multiple distinct pathways. (elsevierpure.com)
  • Cdk5 is a proline-directed serine/threonine kinase, which was first identified as a CDK family member due to its similar structure to CDC2/CDK1 in humans, a protein that plays a crucial role in the regulation of the cell cycle. (wikipedia.org)
  • Moreover, due to the fact that Cdk5 also intervenes in the regulation of synaptic plasticity, it is implicated in the processes of learning and memory formation, as well as the creation of drug addiction. (wikipedia.org)
  • This study focuses on the functional regulation of eNOS by Cdk5/p35 complex in a phosphorylation dependent manner. (drugbank.com)
  • Zhao J, Kennedy BK, Lawrence BD, Barbie DA, Matera AG, Fletcher JA, Harlow E. NPAT links cyclin E-Cdk2 to the regulation of replication-dependent histone gene transcription. (proteopedia.org)
  • 2007. Regulation of protein phosphatase inhibitor-1 by cyclin-dependent kinase 5. . (cornell.edu)
  • Our recent study elucidates the serine-threonine kinase cyclin dependent kinase 5 (Cdk5) as the missing link in the regulation of Foxc2 in the lymphatic endothelium [ 12 ]. (oncotarget.com)
  • The major focus of his research has been to study the mechanisms of topographic regulation of neuronal cytoskeletal proteins regulation by phosphorylation and neurodegeneration. (pathologyresjournal.com)
  • The other project is to study the expression, regulation and role of neuronal Cdk5 in nerve cell function.Cdk5 is a unique multifunctional kinase. (pathologyresjournal.com)
  • The Cdk5-AMT signal pathway involved in regulation of DDR by TIGAR defines a new role of TIGAR in cancer cell survival and it suggests that TIGAR may be a therapeutic target for cancers. (nature.com)
  • Cyclin I-Cdk5 preferentially phosphorylates tau. (jci.org)
  • B ) To compare the kinase kinetics by which cyclin I-Cdk5 phosphorylates tau in comparison with p35-Cdk5, a time-course experiment was performed in HEK293 cells cotransfected with myc-p35 and HA-Cdk5 or myc-cyclin I and HA-Cdk5. (jci.org)
  • The protein encoded by this gene is a neuron-specific activator of CDK5 kinase. (nih.gov)
  • This protein and neuron-specific CDK5 activator CDK5R1/p39NCK5A both share limited similarity to cyclins, and thus may define a distinct family of cyclin-dependent kinase activating proteins. (nih.gov)
  • Synaptic localization of p39, a neuronal activator of cdk5. (nih.gov)
  • We studied here phosphorylation of 5-HT 1A R by cyclin-dependent kinase 5 (Cdk5), a neuron-specific membrane-bound Ser/Thr kinase that is activated by binding of the p35 Cdk5 activator. (elsevierpure.com)
  • Cdk5 is activated by complexing with neuron-specific activator molecules such as p35 and p39. (biomedcentral.com)
  • During these studies I discovered a neuronal cell cycle like kinase now known as Cdk5 and small twenty four amino acid peptide, (TFP5), from the activator of neuronal cell cycle like kinase (Cdk5), p35, drastically reduced the pathological symptoms of neurodegenerative diseases like Alzheimer's, Parkinson's diseases and ALS without toxicity. (pathologyresjournal.com)
  • The molecule belongs to the cyclin-dependent kinase family. (wikipedia.org)
  • Cdk5 is named after its structural similarity to members of the serine/threonine cyclin-dependent kinase family. (frontiersin.org)
  • Examination of the mechanism by which NMDA modulates the phosphorylation of protein phosphatase inhibitor-1 at Ser6 and Ser67 and dopamine- and cAMP-regulated phosphoprotein M r 32 000 at Thr75 revealed that generalized depolarization, rather than specific activation of NMDA receptors, was sufficient to induce decreases in these Cdk5 sites. (elsevierpure.com)
  • NMDA-induced decreases in the phosphorylation of Thr75 of dopamine- and cAMP-regulated phosphoprotein M r 32 000 required protein phosphatase 1/2A activity and extracellular Ca 2+ . (elsevierpure.com)
  • Furthermore, while the decrease in phosphorylation of Ser6 was partially dependent on protein phosphatase 2B, that of Ser67 was independent of the major protein serine/threonine phosphatases, likely indicating the presence of a pathway by which NMDA inhibits Cdk5 activity. (elsevierpure.com)
  • Here DARPP-32 phosphorylation by protein kinase A (PKA), DARPP-32 into a powerful protein phosphatase 1 (PP1) inhibitor. (pp1a.com)
  • Inhibitor-1, the first identified endogenous inhibitor of protein phosphatase 1 (PP-1), was previously reported to be a substrate for cyclin-dependent kinase 5 (Cdk5) at Ser 67 . (elsevierpure.com)
  • Moreover, in the 1-methyl-4-phenyl-1,2,4,6-tetrahydropyridine (MPTP) mouse model, dopamine deficiency caused increased phosphorylation of both Tyr15-Cdk5 and Thr75-DARPP-32 in the striatum, which could be attenuated by administration of L-3,4-dihydroxyphenylalanine and imatinib (STI-571), a selective c-Abl inhibitor. (frontiersin.org)
  • Glutamate inputs activate Cdk5, which inhibits postsynaptic dopamine D1 receptor (D1R)-mediated signaling by phosphorylating DARPP-32 at Thr75 (Thr75-DARPP-32) in the striatum, since DARPP-32 with Thr75 phosphorylation (DARPP-32-pThr75) functions as an inhibitor of cAMP-dependent protein kinase A (PKA). (frontiersin.org)
  • Using a rational drug discovery approach, we recently developed SB1495, a novel reversible covalent inhibitor of the cyclin-dependent kinase 5 (Cdk5)-mediated phosphorylation of PPARγ at Ser245, a key factor in the insulin-sensitizing effect of PPARγ-targeted drugs. (rcsb.org)
  • Roscovitine is a cyclin-dependent kinase (cdk) inhibitor. (tocris.com)
  • Study determined that retinitis pigmentosa has a similar pathogenesis mechanism to Alzheimer disease, and activity of cyclin-dependent kinase 5(Cdk5) and its activators participates in the degeneration of central nervous system.Roscovitine, an inhibitor of Cdk5, can suppress activity of Cdk5/p25 pathway and therefore inhibit cell apoptosis.However, the influence of roscovitine on retinitis pigmentosa(RP) is unclear. (cjeo-journal.org)
  • Basal levels of phospho-Ser 6 inhibitor-1, as detected by a phosphorylation state-specific antibody against the site, existed in specific regions of the brain and varied with age. (elsevierpure.com)
  • Dephosphorylation of inhibitor-1 at Thr 35 is equivalent to inactivation of the protein, as inhibitor-1 only serves as an inhibitor of PP-1 when phosphorylated by cAMP-dependent kinase (PKA) at Thr 35 . (elsevierpure.com)
  • Thus, inhibitor-1 serves as a critical junction between kinase- and phosphatase-signaling pathways, linking PP-1 to not only PKA and calcineurin but also Cdk5. (elsevierpure.com)
  • Glutamate inputs activate cyclin-dependent kinase 5 (Cdk5), which inhibits postsynaptic dopamine signaling by phosphorylating DARPP-32 (dopamine- and cAMP-regulated phosphoprotein, 32 kDa) at Thr75 in the striatum. (frontiersin.org)
  • Inhibits phosphorylation of vimentin in vivo . (tocris.com)
  • Dinaciclib (SCH 727965) inhibits cyclin dependent kinases CDK1, CDK2, CDK5, CDK9, and CDK12, which prevents phosphorylation of downstream targets and may promote cell cycle arrest and apoptosis in cancer cells ( PMID: 24900195 , PMID: 24004674 , PMID: 27880910 , PMID: 32269732 ). (jax.org)
  • The profiling of compound 51 against a panel of 339 kinases revealed high selectivity for CDKs, with preference for CDK2 and CDK5 over CDK9, CDK1, CDK4, and CDK6. (proteopedia.org)
  • These results suggest that Cdk5-p35 modulates 5-HT signaling through phosphorylation-dependent degradation of 5-HT lA R. (elsevierpure.com)
  • Our results suggest a functional link of Cdk5-pTyr15 with postsynaptic dopamine and glutamate signals through the c-Abl kinase activity in the striatum. (frontiersin.org)
  • One of the regulatory mechanisms that modulate the Cdk5 kinase activity is phosphorylation of conserved residues ( Dhavan and Tsai, 2001 ). (frontiersin.org)
  • No kinase activity was detected in the presence of a kinase-inactive dominant negative Cdk5 mutant (HA-D145N-Cdk5, lane 3, 7) or in the absence of either Cdk5 (lanes 1, 5) or p35/cyclin I (lanes 4, 8). (jci.org)
  • Phosphorylation of the AChR β subunit is correlated with increased rapsyn/AChR binding, suggesting that the effect of βY390 phosphorylation on clustering is mediated by rapsyn. (jneurosci.org)
  • Together, these findings suggest that agrin-induced phosphorylation of the β subunit motif increases the stoichiometry of rapsyn binding to the AChR, thereby helping to stably cluster the receptor and anchor it at high density in the postsynaptic membrane. (jneurosci.org)
  • Indeed, we have shown previously that mutation of a tyrosine phosphorylation site in the long cytoplasmic loop of the β subunit impairs agrin-induced cytoskeletal anchoring and aggregation of mutant AChR in muscle cells ( Borges and Ferns, 2001 ). (jneurosci.org)
  • Assignment of CDK5R2 coding for the cyclin-dependent kinase 5, regulatory subunit 2 (NCK5AI protein) to human chromosome band 2q35 by fluorescent in situ hybridization. (nih.gov)
  • Repetitive CP exposure increased striatal PKA-dependent phosphorylation of the D1 dopamine receptor downstream effector Ser845 of the GluR1 subunit of the AMPA receptor. (cdc.gov)
  • controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. (proteopedia.org)
  • We here report that Cdk5 with Tyr15 phosphorylation (Cdk5-pTyr15) is enriched in the mouse striatum, where dopaminergic stimulation inhibited phosphorylation of Tyr15-Cdk5 by acting through the D2 class dopamine receptors. (frontiersin.org)
  • This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). (thermofisher.com)
  • a biomarker consisting of the phosphorylation of the retinoblastoma protein (Rb) on serine 249 combined with elevated p39 expression. (nih.gov)
  • Compound 51 inhibited the proliferation of 13 out of 15 cancer cell lines with IC50 values between 0.27 and 6.9 muM, which correlated with the complete suppression of retinoblastoma phosphorylation and the onset of apoptosis. (proteopedia.org)
  • Sema 3A mediated retraction appears to be related to its binding to Npn 1 and Plxn A2 receptors, thus activating the downstream Fyn tyrosine kinase pathway that promotes the threonine-serine kinase cyclin-dependent kinase 5, CDK5, phosphorylation at the Tyr15 residue and the CDK5 processing to generate the active fragment p35. (biomedcentral.com)
  • c-Abelson tyrosine kinase (c-Abl) is known to phosphorylate Cdk5 at Tyr15 (Tyr15-Cdk5) and thereby facilitates the Cdk5 activity. (frontiersin.org)
  • Our results showed that Cdk5 can phosphorylate eNOS both in vitro and in vivo. (drugbank.com)
  • Together, our data suggest that Cdk5 can phosphorylate eNOS at the Ser-113 site and down-regulate eNOS-derived NO levels. (drugbank.com)
  • Cyclin-dependent kinase 5 (Cdk5) is a member of the Cdk family of serine/threonine kinases and is abundantly expressed in the brain ( Dhavan and Tsai, 2001 ). (frontiersin.org)
  • Cyclin-dependent kinases (CDKs) are serine/threonine protein kinases that act as key regulatory elements in cell cycle progression. (proteopedia.org)
  • Cyclin-dependent kinase 5 (Cdk5) is a proline-directed serine/threonine kinase that belongs to the CDK family and is expressed primarily in the central nervous system. (biomedcentral.com)
  • Even though Cdk5 has a similar structure to other cyclin-dependent kinases, its activators are highly specific (CDK5R1 and CDK5R2). (wikipedia.org)
  • If there is a high activity of Cdk5 during brain development, its activators will have a high expression. (wikipedia.org)
  • attenuates abnormally increased striatal phosphorylation of both Tyr15-Cdk5 and Thr75-DARPP-32, as does L-3,4-dihydroxyphenylalanine (L-dopa). (frontiersin.org)
  • 5-HT 1A R was phosphorylated by the Cdk5-p35 complex at Thr314 in the third cytoplasmic loop. (elsevierpure.com)
  • In the striatum, Cdk5 plays a regulatory role in the dopamine and glutamate transmissions that are integrated by DARPP-32 (dopamine- and cAMP-regulated phosphoprotein, 32 kDa) ( Greengard, 2001 ). (frontiersin.org)
  • however, it is not fully understood how Cdk5 interacts with dopamine signaling in the striatum. (frontiersin.org)
  • This strategic localization of Cdk5-pTyr15 suggests a possible role of the c-Abl/Cdk5 signaling in modulation of postsynaptic dopamine and glutamate transmissions in the striatum. (frontiersin.org)
  • In the striatum, basal in vivo phosphorylation and dephosphorylation of Ser 6 were mediated by Cdk5, PP-2A, and PP-1, respectively. (elsevierpure.com)
  • Activation of the D1 dopamine receptor/cAMP/PKA cascade is regulated by the neuronal protein kinase, Cdk5. (cdc.gov)
  • Kinases are enzymes that catalyze reactions of phosphorylation. (wikipedia.org)
  • Phosphorylations are of vital importance during glycolysis, therefore, making kinases an essential part of the cell due to their role in the metabolism, cell signaling, and many other processes. (wikipedia.org)
  • Cdk5 belongs to the eukaryotic protein kinases (ePKs). (wikipedia.org)
  • Unlike other cyclin-dependent kinases, CDK5 does not also require phosphorylation on the T loop. (wikipedia.org)
  • GSK3β and Cdk5 are the two kinases in the center of research on Alzheimer's disease (AD), involved in the pathological symptoms of AD, Aβ plaque formation, tau hyperphosphorylation and neurodegeneration. (frontiersin.org)
  • So far, both kinases have mostly been examined in isolation, leading to a schism of the research field into defenders of the GSK3β-versus the Cdk5 hypotheses of AD. (frontiersin.org)
  • Development of Highly Potent and Selective Diaminothiazole Inhibitors of Cyclin-Dependent Kinases. (proteopedia.org)
  • Although all the substrates, kinases, phosphatases and their regulators are synthesized in neuronal cell bodies, little or no cytoskeletal protein phosphorylation has been detected in the cell body compartment. (pathologyresjournal.com)
  • With his lab, he has shown that the most abundant and extensively phosphorylated motifs in the c-terminal domains (lys-ser-pro-, or KSP) are primarily phosphorylated by proline directed kinases, cdk5 and MAP kinases. (pathologyresjournal.com)
  • Furthermore, phosphoproteomic data and signalome analysis of perihematomal brain tissues suggested that calmodulin-dependent protein kinases 2 (CAMK2A) kinase substrate pairs were significantly downregulated in ICH with HHcy compared with autologous blood injection only, both western blot and immunofluorescence staining confirmed this finding. (bmj.com)
  • Then, our findings further indicate that Hcy significantly downregulates the phosphorylation level of calmodulin-dependent protein kinases 2 (CAMK2A) after ICH, which is associated with neurite outgrowth. (bmj.com)
  • Phosphorylation of endothelial nitric oxide synthase (eNOS) is key mechanism in response to various forms of cellular stimulation. (drugbank.com)
  • Kv2.1 exhibits robust expression across many neuron types and is unique in its conditional role in modulating intrinsic excitability through changes in its phosphorylation state, which affect Kv2.1 expression, localization, and function. (jneurosci.org)
  • Altered phosphorylation of CREB phosphopeptide, a substrate of GSK3β, suggested that GSK3β may be the mediator of Cdk5 activity in kinesin-mediated axonal transport. (frontiersin.org)
  • CDK5 is required for proper development of the brain, and to be activated, it must associate with CDK5R1 or CDK5R2. (wikipedia.org)
  • A ) HEK293 cells were transfected with HA-Cdk5, then cotransfected with either myc-p35 or myc-cyclin I. Following an IP to the myc epitope tag on either p35 or cyclin I, in vitro kinase assays were performed using either histone H1 (HH1) or tau as substrates. (jci.org)
  • Histone H1 and tau (lanes 2, 6) were strongly phosphorylated by p35-Cdk5. (jci.org)
  • In contrast, cyclin I-Cdk5 preferentially phosphorylated tau (lane 6) rather than histone H1 (lane 2). (jci.org)
  • Recently Cdk5 has emerged as an essential kinase in sensory pathways. (wikipedia.org)
  • Since glucocorticoids are essential for life, other cellular signaling pathways strongly regulate GR actions in many different ways, such as physical interaction via their effector transcription factors and epigenetic modifications including phosphorylation and acetylation. (brainimmune.com)
  • Treatment of striatal slices with CP induced a dose-dependent increase in phospho-Ser845 GluR1 and phospho-Thr34 DARPP-32, which was blocked by a D1 antagonist. (cdc.gov)
  • In order to perform, Cdk5 needs to be activated by p35 (these 3 amino acids, Asp-259, Asn-266, and Ser-270, are involved in the formation of hydrogen bonds with Cdk5) or p39 (the isoform of p35), which are two of its neuron-specific regulatory subunits. (wikipedia.org)
  • Most interestingly, the nitrite production was significantly reduced in eNOS and Cdk5/p35 co-transfected SH-SY5Y cells when compared with co-transfection of Cdk5/p35 and S113A. (drugbank.com)
  • Interestingly, brain slice treatment with CP induced calpain-dependent conversion of the Cdk5 activating cofactor p35 to p25, a neuronal injury marker. (cdc.gov)
  • As a matter of fact, when studies were conducted on mice without p35 and p39, the results were the same as the ones observed on mice without Cdk5: there were clear disruptions of the laminar structures in the cerebral cortex, the olfactory bulb, the hippocampus, and the cerebellum. (wikipedia.org)
  • Recent evidence from p25 transgenic mice suggests that there is a dynamic crosstalk: during aging or prolonged overactivation of Cdk5, GSK3β activity may alter in favor of AD pathogenesis. (frontiersin.org)
  • Several lines of conditional Cdk5 knockout mice (KO) have been generated and have shown different outcomes for learning and memory. (biomedcentral.com)
  • Furthermore, NMDAR-dependent long-term depression (LTD) induction by low-frequency stimuli in hippocampal slices from CamkII-p35cKO mice was impaired compared to that in control mice. (biomedcentral.com)
  • The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated tau. (abcam.cn)
  • Indeed, we found that rapsyn associated with CD4-β loop chimeras in a phosphorylation-dependent manner, and that agrin increased the ratio of rapsyn binding to wild type AChR but not to AChR-β 3F/3F , which lacks β loop tyrosine phosphorylation sites. (jneurosci.org)
  • Violin plots show distribution of expression levels for Dual specificity tyrosine-phosphorylation-regulated kinase 2 (SMED30002728) in cells (dots) of each of the 12 neoblast clusters. (stowers.org)
  • Expression of Dual specificity tyrosine-phosphorylation-regulated kinase 2 (SMED30002728) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells. (stowers.org)
  • Violin plots show distribution of expression levels for Dual specificity tyrosine-phosphorylation-regulated kinase 2 (SMED30002728) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells. (stowers.org)
  • phosphorylation of Tyr216 can enhance enzyme activity, and conversely phosphorylation of Ser9 is inhibitory. (frontiersin.org)
  • However, the climbing fibre input continuously exerts an activity-dependent repression on parallel fibre synapses, thus confining them to the distal Purkinje cell dendritic domain. (123dok.org)
  • Cyclin-dependent kinase 5 is a protein, and more specifically an enzyme, that is encoded by the Cdk5 gene. (wikipedia.org)
  • The gene Cdk5 contains 12 exons in a region that contains around 5000 nucleotides (5kb), as it was determined by Ohshima after cloning the Cdk5 gene that belonged to a mouse. (wikipedia.org)
  • Cyclin-dependent kinase 5 (Cdk5) /p35 is involved in many developmental processes of the central nervous system. (biomedcentral.com)
  • Phosphorylation of RB1 disturbs its interaction with E2F1. (proteopedia.org)
  • In summary, our study elucidates the Cdk5-Foxc2 interaction as a critical regulator of lymphatic vessel development. (oncotarget.com)
  • Here, we define a phosphorylation-dependent binding site on the receptor that mediates agrin-induced clustering. (jneurosci.org)
  • Cyclin-dependent kinase 5 promotes proteasomal degradation of the 5-HT 1A receptor via phosphorylation Biochemical and Biophysical Research Communications , 510 (3), 370-375. (elsevierpure.com)
  • These diverse actions of glucocorticoids are mediated by the glucocorticoid receptor (GR), which belongs to the nuclear receptor superfamily and functions as a hormone-dependent transcription factor. (brainimmune.com)
  • Cyclin-dependent kinase 5 (CDK5) affects the tumor microenvironment, which may influence the efficacy of TKI+IO. (e-crt.org)
  • Using immunohistochemistry, expression of DARPP-32, PP1 and Cdk5 is determined in a large cohort of primary tumors from ovarian cancer patients (n = 428, 445 and 434 respectively) to evaluate the relationship between clinical outcome and clinicopathological criteria. (pp1a.com)
  • Drepper F, Biernat J, Kaniyappan S, Meyer HE, Mandelkow EM , Warscheid B, Mandelkow E . A combinatorial native MS and LC-MS/MS approach reveals high intrinsic phosphorylation of human Tau but minimal levels of other key modifications. (neurotree.org)
  • In the course of the disease, tau changes its phosphorylation state and becomes hyperphosphorylated, gets truncated by proteolytic cleavage, is subject to O-glycosylation, sumoylation, ubiquitinylation, acetylation and some other modifications. (microbialcell.com)
  • The hinge regions are proteolyzed by Ca 2+ -dependent calpains, and the cleavage of these sites produces a 90 kDa C -terminal fragment of FLNA (FLNA CT ) and a 200 kDa N -terminal fragment (FLNA NT ) [ 6 ] . (encyclopedia.pub)
  • Also, Cdk5 collaborates with Reelin signaling in order to assure the proper neuronal migration in the developing brain. (wikipedia.org)
  • Cdk5 is not only implicated in neuronal migration. (wikipedia.org)
  • Cdk5/p35 is also implicated in synaptic plasticity, learning and memory. (biomedcentral.com)
  • In vitro kinase assay together with the bioinformatic analysis and site direct mutagenesis revealed that Ser-113 is the major phosphorylation site for Cdk5. (drugbank.com)
  • Hyper-phosphorylation of Rb S249 together with CDK5R2/p39 overexpression are associated with impaired cell adhesion and epithelial-to-mesenchymal transition: Implications as a potential lung cancer grading and staging biomarker. (nih.gov)
  • RFscore based on CDK5 may be utilized as a biomarker to determine the optimal treatment strategy. (e-crt.org)
  • Deletion of Cdk5 in the mouse endothelium results in severe lymphedema formation and embryonic lethality. (oncotarget.com)
  • Cdk5 has 292 amino acids and presents both α-helix and β strand structures. (wikipedia.org)
  • In the high CDK5 subgroup, CD8 + T cells revealed a dysfunction phenotype with decreased granzyme B, and more regulatory T cells were identified. (e-crt.org)
  • cyclin-dependent kinase 5, regulatory. (wikigenes.org)