p16 Ink4a downregulation leads to an activation of PKA-CREB-PGC1α signaling through increased phosphorylation of PKA regulatory subunits. (diabetesjournals.org)
Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle. (bdbiosciences.com)
The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins including p21 and p27Kip1. (bdbiosciences.com)
Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. (caslab.com)
A cyclin-dependent protein kinase holoenzyme complex that has a cyclin B regulatory subunit and a Cdk1 kinase subunit. (proconsortium.org)
and Atg13, the regulatory subunit of the Atg1p signaling complex that stimulates Atg1p kinase activity and is essential for vesicle formation for the duration of autophagy and cytoplasm-to-vacuole targeting. (atminhibitor.com)
Repetitive cell cycles, which are essential to the perpetuation of life, are orchestrated by an underlying biochemical reaction network centered around cyclin-dependent protein kinases (Cdks) and their regulatory subunits (cyclins). (ox.ac.uk)
CK2 is traditionally considered to be a tetrameric enzyme, composed of two catalytic subunits and two regulatory subunits, which are encoded in yeast by CKA1 and CKA2 genes, CKB1 and CKB2 genes respectively. (unimib.it)
Deletion of regulatory subunits, or of either catalytic subunit gene alone has few phenotypic effects, but simultaneous disruption of both CKA1 and CKA2 genes is lethal. (unimib.it)
The CPC includes, as well as Aurora B, three regulatory subunits: the inner centromeric protein, Survivin, and Borealin/Dasra W. Beginning in prophase, the CPC localizes to condensing chromosomes and steadily concentrates at the internal centromere where one function would be to correct poor Infectious causes of cancer spindle kinetochore devices. (smadpathway.com)
Cdc55 counteracts the Cdk1 phosphorylation of the APC/C subunit Cdc16 [ 11 ] [ 10 ] , keeping the spindle checkpoint assembly (SAC) active until the cell is prepared for anaphase. (encyclopedia.pub)
controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. (wuxibiortus.com)
Moreover, the rapidity of Whi5 hyper-phosphorylation in late G1 depends on 'priming' sites that dock the Cks1 subunit of Cln1,2-Cdk1 complexes. (bvsalud.org)
Consistent with this, it encompassed known regulated phosphorylation websites for example Thr142 of your transcriptional activator Msn4, which has been shown to reduce in response to osmotic stress (49), and Ser530 on the deubiquitylase Ubp1, a identified Cdk1 substrate (50). (atminhibitor.com)
Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). (biossusa.com)
CDC22
CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily.CDC2 plays a key role in the control of the eukaryotic cell cycle. (caslab.com)
CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF) , which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. (caslab.com)
Phosphorylates6
Wee1 phosphorylates the Tyr-15 residue of CDK1, rendering MPF inactive. (wikipedia.org)
We report that Whi5 is a poor substrate of Cln3-Cdk1, which instead phosphorylates the RNA polymerase II subunit Rpb1's C-terminal domain on S5 of its heptapeptide repeats. (bvsalud.org)
CK2 also phosphorylates two key regulators of the G1/S transition in yeast: Sic1, the Cdk1-Clb5/6 inhibitor, which is phosphorylated by CK2 on Ser201 (Coccetti et al. (unimib.it)
E ) Cdk1 Clb2 phosphorylates the central region of Esp1 in vitro . (figshare.com)
Protein kinases1
Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. (wuxibiortus.com)
Cyclins1
The cyclins are necessary for the kinase subunit to function with the appropriate substrate. (wikipedia.org)
Catalytic subunits1
In Saccharomyces cerevisiae a specialization of the two catalytic subunits (α e α') was shown: α subunit is involved in cell polarity, while α' subunit is linked to cell-cycle regulation and was shown to be fundamental both in G1 phase and in mitosis. (unimib.it)
Inhibitor1
Quite interestingly, we found that a CDK inhibitor, Far1, could regulate the Cln2-CDK1 activity partly by inhibiting the Cln2-substrate interaction, thus demonstrating that docking interactions can be targets of regulation. (umassmed.edu)
Binds3
SGO1 binds to a composite interface formed by the SA2 and SCC1RAD21 subunits of cohesin. (cbinsights.com)
Cln3-Cdk1 binds SBF-regulated promoters and Cln3's function can be performed by the canonical S5 kinase Ccl1-Kin28 when synthetically recruited to SBF. (bvsalud.org)
This region binds the B55 subunit of PP2A and, according to our predicted models, it might involve its substrate-binding groove. (ed.ac.uk)
PP2A(B55) is the master regulator of mitotic exit, as it triggers late mitotic events by counteracting Cdk1 activity. (ed.ac.uk)
Complex4
SNF5 is a core subunit of the SWI/SNF chromatin-remodeling complex. (umassmed.edu)
In late-G1, Whi5 is rapidly hyper-phosphorylated by Cln1,2 in complex with the cyclin-dependent kinase Cdk1. (bvsalud.org)
In budding yeast, the G1 cyclin Cln3-Cdk1 complex is thought to directly phosphorylate the Whi5 protein, thereby releasing the transcription factor SBF and committing cells to division. (bvsalud.org)
2007), explained for the first time the molecular mechanism of the G1 block due to CK2 inactivation: Sic1 accumulation inhibits Cdk1-Clb5 complex, thus preventing the onset of DNA replication. (unimib.it)
Gene2
In humans, mRNA expression of hTERT ( hEST2 ), the candidate telomerase catalytic subunit gene, appears to parallel the levels of telomerase enzyme activity, suggesting that induction of hTERT is necessary and perhaps sufficient for expression of telomerase activity in tumor cells. (nature.com)
This gene encodes a ribosomal protein that is a component of the 60S ribosome subunit. (nih.gov)
PP2A1
Our ongoing work hints that this specificity is mediated by dynamic interactions between Sgo1 and the B56 subunits, and aims to decipher the functional contribution of these interactions in achieving faithful chromosome segregation My work also unveils a novel interaction between PP2A(B56) and the CPC. (ed.ac.uk)
Cdk21
Cyclin A is involved in both S-phase and G2/M transitions of the cell cycle through its association with cdk2 and cdk1, respectively. (bdbiosciences.com)
Dimeric1
FLNa dimeric subunits have N-terminal spectrin family F-actin binding domains (ABDs) and an elongated flexible segment of 24 immunoglobulin (Ig) repeats. (rupress.org)
Antibody1
Esp1 was immunoprecipitated from the strains in (C) growing asynchronously, incubated with γ-[ 32 P]ATP and purified Cdk1 Clb2-CBP , washed, run on a polyacrylamide gel, and exposed to a phosphorimager screen or immunoblotted with anti-Esp1 antibody. (figshare.com)
Promotes1
As the concentration of Cyclin B/CDK1 increases, the heterodimer promotes APC to polyubiquitinate Cyclin B/CDK1. (wikipedia.org)
Bind1
The CDK1 subunit is now free and can bind to cyclin B, activate MPF, and make the cell enter mitosis. (wikipedia.org)
We conclude that synthesis of the hTERT telomerase subunit represents the rate-limiting determinant of telomerase activity in these cells and that this protein, once expressed, becomes part of the functional telomerase holoenzyme. (nature.com)
Dephosphorylation1
In addition, two other residues on the CDK1 subunit must be activated by dephosphorylation. (wikipedia.org)
Mitosis2
Oscillations of Cdk1/CycB activity between low and high levels during the cycle trigger DNA replication and mitosis in the correct order. (ox.ac.uk)
Along with Cdk1/cyclin N, members of the Aurora/Ipl1 kinase family are also crucial regulators of mitosis. (smadpathway.com)
Activity1
We showed that the observed increase of Sic1 level was responsible for an inhibition of Cdk1-Clb5 kinasic activity. (unimib.it)
Esp12
B ) Esp1 contains six minimal Cdk1 consensus sites (S/TP): S13 and T16 (termed N-terminal ), T1014, S1027 and T1034 (termed central ) and S1280 (termed C-terminal ). (figshare.com)
Esp1 was immunoprecipitated from wild-type cells and phosphorylated with purified Cdk1 Clb2-CBP and γ-[ 32 P]ATP while immobilized on IgG-coupled magnetic beads. (figshare.com)
Cell1
Methods: We investigated ATM, ATR, and DNA-PKcs expressions in ovarian epithelial cancers [protein expression (n = 194 patients), mRNA expression (n = 156 patients)] and correlated to clinicopathological outcomes as well as expression of X-ray repair cross-complementing protein 1 (XRCC1), cell division cycle-45 (CDC45), cyclin-dependent kinase 1(CDK1) and Ki-67 in tumours. (ntu.ac.uk)
Nuclear1
We used yeast strains expressing TAP-tagged CK2 subunits, and we showed that neither total levels of the four subunits, nor their subcellular localization (which is mainly nuclear both in glucose and in ethanol) were modulated by carbon source. (unimib.it)
Transition1
During the transition of G2 to M phase, cdk1 is de-phosphorylated by CDC25. (wikipedia.org)
Levels1
INTERPRETATION: These results demonstrated that an RBD-Fc protein subunit/MF59® adjuvanted vaccine can induce high levels of broadly reactive nAbs, including when used as a booster following prior immunisation of mice with whole ancestral-strain spike vaccines. (bvsalud.org)