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  • CDK2
  • Cyclin-dependent kinase 2, also known as cell division protein kinase 2, is an enzyme that in humans is encoded by the CDK2 gene. (wikipedia.org)
  • This protein associates with and is regulated by the regulatory subunits of the complex including cyclin E or A. Cyclin E binds G1 phase Cdk2, which is required for the transition from G1 to S phase while binding with Cyclin A is required to progress through the S phase. (wikipedia.org)
  • The role of this protein in G1-S transition has been recently questioned as cells lacking Cdk2 are reported to have no problem during this transition. (wikipedia.org)
  • However, in recent years, new evidence proved that the presence of CDK6 is not essential for proliferation in every cell type, the cell cycle has a complex circuitry of regulation and the role of CDK6 might be more important in certain cell types than in others, where CDK4 or CDK2 can act as protein kinases compensating its role. (wikipedia.org)
  • On the onset of the S phase, Cdc6p gets phosphorylated by the Cdc28-Clb5-Clb6 complex (Cdk2) and consequently becoming inactivated. (wikipedia.org)
  • genes
  • The fact that the strain in which all six CLB genes are deleted is viable if Clb1p is overexpressed indicates that this specificity can be subverted by overexpression of a single Clb protein ( H aase and R eed 1999 ). (genetics.org)
  • The Saccharomyces cerevisiae PAH1-encoded phosphatidate phosphatase (PAP) catalyzes the penultimate step in the synthesis of triacylglycerol and plays a role in the transcriptional regulation of phospholipid synthesis genes. (semanticscholar.org)
  • Together, these transcription factors promote the expression of over 200 genes, which encode the proteins necessary for carrying out the biochemical activities of S-phase. (wikipedia.org)
  • substrate
  • Several lines of evidence point to the Mcm complex as the substrate of Cdc7p kinase. (genetics.org)
  • Cdh1 is one of the co- activator proteins of APC/c and therefore contributes to the regulation of protein degradation, by providing substrate specificity to the E3-ligase in a cell cycle regulated manner. (wikipedia.org)
  • Clb5
  • Protein expression was followed by Western blot with cells containing PDS1-3HA and CLB5-TAP (A and B) or CLB5-9MYC tagged (C and D). Cells were grown in media containing 2% raffinose and galactose (Raff/Gal) to induce the expression of Cdc20 ( ADE2-pGAL1-CDC20-13MYC ). (genetics.org)
  • Genetic
  • This was accomplished through a genetic and molecular analysis of the mechanism by which the mcm5-bob1 mutation bypasses the function of the Cdc7p/Dbf4p kinase. (genetics.org)
  • Genetic evidence suggested that the inhibition of mitotic cyclin-dependent kinase activities was dependent on and possibly mediated through the CDC6 gene product. (mysciencework.com)
  • CKS2
  • The CKS2 mRNA is found to be expressed in different patterns through the cell cycle in HeLa cells, which reflects specialized role for the encoded protein. (nih.gov)
  • Patients with positive-CKS2 protein expression had a poorer five year survival frequency than patients who did not express CKS2 protein. (nih.gov)
  • phosphatase
  • PAP is phosphorylated at multiple Ser and Thr residues and is dephosphorylated for in vivo function by the Nem1p-Spo7p protein phosphatase complex localized in the nuclear/endoplasmic reticulum membrane. (semanticscholar.org)
  • The protein phosphatase Cdc14 dephosphorylates Cdh1 and therefore activates APCCdh1. (wikipedia.org)
  • PLK1
  • Suppression of Cdh1 by RNA interference leads to an aberrant accumulation of APCCdh1 target proteins, such as cyclin A and B, the kinase AuroraB, PLK1, Skp2 and Cdc20, another APC/c co- activator. (wikipedia.org)
  • PHASE
  • The protein is nuclear in G1 cells but cytoplasmic in S, G2, and M phase cells. (ox.ac.uk)
  • Molecular experiments revealed that the mcm5-bob1 mutation allows for constitutive loading of Cdc45p at early origins in arrested G1 phase cells when both kinases are inactive. (genetics.org)
  • A MADS box protein consensus binding site is necessary and sufficient for activation of the opaque-phase-specific gene OP4 of Candida albicans. (naver.com)
  • Furthermore, Cdc6 indirectly inhibits activation of the p34cdc2/CDC28 M phase kinase, thus nuclear division is suppressed. (wikipedia.org)
  • complex
  • In the N-terminal region at amino acid position 55-61 the cdh1 protein contains a C-Box motif, which is required for the association with the APC/c complex. (wikipedia.org)
  • This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). (wikipedia.org)
  • The protein complex SCFβ-TrCP1/2 is an E3 ubiquitin ligase that functions in Wee1A ubiquitination. (wikipedia.org)
  • After the complex is formed, the C-CDK6 enzymatic complex phosphorylates the protein pRb. (wikipedia.org)
  • Cdc6p is an ATP binding protein and a member of the pre-replicative complex (pre-RC) together with the origin recognition complex (ORC), Cdt1 and the MCM complex (containing MCM2-7p). (wikipedia.org)
  • From studies with E. coli γ clamp loading complex, it was suggested that domain III mediates protein-protein interactions with other AAA+ ATPases in the pre-RC, thus suggesting that the Cdc6p builds a homodimer in its native form. (wikipedia.org)
  • Once cyclin-dependent kinases bind to cyclin, the formed complex is in an activated state. (wikipedia.org)
  • nuclear
  • As all three serines are phosphorylated by purified CDC28-dependent H1 kinase activity in vitro, we propose a model in which the CDC28 kinase acts directly to control nuclear entry of SWI5. (ox.ac.uk)
  • yeast
  • Here we report a green fluorescent protein−based quantitative chromosome transmission fidelity (qCTF) assay in budding yeast that allows sensitive and quantitative detection of CIN and can be easily adapted to high-throughput analysis. (g3journal.org)
  • Together with Kelly Tatchell he cloned the S. cerevisiae mating-type locus and found, surprisingly, that 'silent' copies of the mating-type genes including their promoters are maintained in the yeast chromosome. (wikipedia.org)
  • It has been highly studied in the budding yeast S. cerevisiae, and the fission yeast S. pombe, where it is encoded by genes cdc28 and cdc2, respectively. (wikipedia.org)
  • Her research group recently created haploid yeast cells containing extra copies of chromosomes and discovered that these aneuploid strains elicit phenotypes independent of the identity of the additional chromosome such as defects in cell cycle progression, increased energy demands, and interference with protein biosynthesis. (wikipedia.org)
  • Low Cdc28-Clb activity leads to the disassembly of the mitotic spindle, the assembly of the prereplicative complex and initiation of bud formation in yeast. (wikipedia.org)
  • Fission yeast also contains a second kinase Mik1 that can phosphorylate the tyrosine. (wikipedia.org)
  • The Cln3 protein is a budding yeast G1 cyclin that controls the timing of Start, the point of commitment to a mitotic cell cycle. (wikipedia.org)
  • cln1
  • We have isolated three Candida genes that can complement the inability of S. cerevisiae cln1 mutant strains to form filaments. (uci.edu)
  • Furthermore, Cln3 was a stronger activator Start transit than Cln1 and Cln2, even though Cln3-CDK had an inherently weaker kinase activity than the other Clns. (wikipedia.org)
  • Cdk1
  • To identify additional factors necessary for the inhibition of the Cdk1/Cdc28 kinase in G1, we isolated mutants that can replicate DNA in the absence of Cdc4 function. (mendeley.com)
  • This transcriptional control may be important for inactivation of the Clb/Cdk1 kinase in G2/M transition and during the subsequent G1 period. (mendeley.com)
  • Cdk1 is a small protein (approximately 34 kilodaltons), and is highly conserved. (wikipedia.org)
  • Cdk1, like other kinases, contains a cleft in which ATP fits. (wikipedia.org)
  • Cdk1 also contains a PSTAIRE helix, which, upon cyclin binding, moves and rearranges the active site, facilitating Cdk1 kinase activities. (wikipedia.org)
  • Cdk1 activity is best understood in S. cerevisiae, so Cdk1 S. cerevisiae activity is described here. (wikipedia.org)
  • cleft
  • The active site, or ATP-binding site, of all kinases is a cleft between a small amino-terminal lobe and a larger carboxy-terminal lobe. (wikipedia.org)
  • anaphase
  • As a student under Nasmyth, Amon demonstrated that CDC28 protein kinase is not required for the metaphase to anaphase transition and CLB2 proteolysis continues until reactivation of CDC28 toward the end of G1. (wikipedia.org)
  • mitotic
  • In many eucaryotic cells, the midzone of the mitotic spindle forms a distinct structure containing a specific set of proteins. (pubmedcentralcanada.ca)
  • ACT3: a putative centractin homologue in S. cerevisiae is required for proper orientation of the mitotic spindle. (pubmedcentralcanada.ca)
  • Au Yong JY, Wang YM, Wang Y (2016) The Nim1 kinase Gin4 has distinct domains crucial for septin assembly, phospholipid binding and mitotic exit. (springer.com)
  • UniProt
  • p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later. (uniprot.org)