Cdc20 proteins. Medical search. Frequent questions

A closely related protein, Cdc20homologue-1 (Cdh1) plays a complementary role in the cell cycle. (wikipedia.org)
APC/C activity is dependent on CDC20 (and Cdh1), because CDC20 often binds the APC/C substrates directly. (wikipedia.org)
In fact, it is thought that CDC20 and Cdh1 (see below) are receptors for the KEN-box and D-box motifs on substrates. (wikipedia.org)
CDC20-homologue 1 (Cdh1) plays a complementary role to CDC20 in cell cycle progression. (wikipedia.org)
Anaphase-promoting complex (APC) is activated by two regulatory proteins, Cdc20 and Cdh1. (elsevierpure.com)
The model starts at mitotic entry initiated by the activities of Cyclin-dependent kinase 1 (CDK1) and Polo-like kinase 1 (PLK1), transitions through Anaphase-promoting complex (APC/C) bound to Cell division cycle protein 20 (CDC20), and ends upon mitotic exit mediated by APC/C bound to CDC20 homolog 1 (CDH1). (pharmafocusasia.com)
and (4) inclusion of the APC/C, CDC20 and CDH1 interactions. (pharmafocusasia.com)
Cdc20/Fzy and Cdh1/ Fzr are substrate-specific APC/C activators. (biu.ac.il)
The activator Cdc20 is then replaced by a second activator, Cdh1, and APC/C Cdh1 promotes complete degradation of M cyclin, followed by polo-like kinase 1, Aurora A, and other substrates, to complete mitosis and cytokinesis and drive progression into G1 [ 1 , 2 ]. (biomedcentral.com)

Unattached or tensionless kinetochores activate the checkpoint and enhance the production of the mitotic checkpoint complex (MCC) consisting of BubR1, Bub3, Mad2, and Cdc20. (elsevierpure.com)
The N-terminal region of BubR1 binds to both Cdc20 and Mad2, thus nucleating MCC formation. (elsevierpure.com)
MAD2, standing for mitotic arrest deficient 2, is a spindle checkpoint protein that plays an integral role in the human body. (prospecbio.com)
There are several interactions that MAD2 shares with others, specifically, binding with Cdc20, in a vital protein-to-protein interaction. (prospecbio.com)
This phenotype is associated with interaction of E2 with the Mitotic Checkpoint Complex (MCC) proteins Cdc20, MAD2 and BUBR1. (docksci.com)
M2I-1 is a protein-protein interaction inhibitor targeting the binding of Mad2 to Cdc20, an essential proteinprotein interaction (PPI) within the SAC. (americanchemicalsuppliers.com)
Shugoshin is a Mad1/Cdc20-like interactor of Mad2. (nature.com)

CDC20 is a protein related to the beta subunit of heterotrimeric G proteins. (wikipedia.org)
K can From a variety of extracellular Ren stimuli Ren Ren signals such as growth factors, ligands for G-protein-coupled receptors, cytokines, and vitamin D3, the p85 subunit of regulation at the plasma membrane, such as catalytic subunit recruit p110 PI P2 4 5 PI converts 3.4, 5 P3. (microrna1.com)

Progression through mitosis is driven by a complex set of protein-protein interaction reactions and their regulations that change over time. (pharmafocusasia.com)
Here we present an overview of the protein phosphatase 2A family's roles during mitosis. (encyclopedia.pub)
The Cell cycle study combines topics in areas such as Ploidy, Mitosis, Drosophila Protein and Cell fate determination. (research.com)
The Anaphase-Promoting Complex/Cyclosome (APC/C) ubiquitin ligase mediates degradation of cell cycle proteins during mitosis and G1. (biu.ac.il)
Here, we used experimental and computational approaches to dissect the quantitative mechanisms underlying the ordered degradation of the substrates of the ubiquitin ligase APC/C Cdc20 , a key regulator of chromosome segregation in mitosis. (biomedcentral.com)
It encodes a protein that prevents separin from promoting sister chromatid separation during mitosis [ 3 ]. (biomedcentral.com)

However, the most important interaction of CDC20 is with the Anaphase Promoting Complex. (wikipedia.org)
The middle region of human BubR1 (BubR1M) also interacts with Cdc20, but the nature and function of this interaction are not understood. (elsevierpure.com)
Thus, the BubR1M-Cdc20 interaction indirectly contributes to MCC homeostasis. (elsevierpure.com)
The protein-protein interaction networks of the DEGs were constructed according to the data from the STRING database. (hindawi.com)
Traditional bioinformatics methods including gene ontology (GO) and pathways enrichment analyses of DEGs combined protein-protein interaction (PPI) network and cytohubber calculating software as well as Molecular Complex Detection (MCODE) analysis were used to analyze the key gene and pathway. (hindawi.com)
This gene and/or its encoded proteins are associated with 219 experimentally validated interaction(s) in this database. (innatedb.com)
The cellular protein-protein interaction network that governs cellular proliferation (cell cycle) is highly complex. (pharmafocusasia.com)
We have developed a novel integrated computational model of human mitotic cell cycle for the purpose of understanding how the human mitotic protein-protein interaction network (Fig 1A) is altered during disease. (pharmafocusasia.com)
Using a computational model based on multi-step ubiquitination, we then show how changes in the interaction between a single substrate and APC/C Cdc20 can alter the timing of degradation onset relative to APC/C Cdc20 activation, while ensuring a fast degradation rate. (biomedcentral.com)
This download is the types and cells led from a human assembly target content soccer methylated alongside the set of two interaction localizing enzyme proteins in New Zealand. (evakoch.com)
To demonstrate the utility of CompNet, we have compared a (time-series) human gene-expression dataset, post-infection by two strains of Mycobacterium tuberculosis , overlaid on the human protein-protein interaction network. (biomedcentral.com)

Cell division cycle protein 20 homolog is a protein in humans that is encoded by CDC20 gene. (cusabio.com)

Anaphase-promoting complex that intertwine with fields like Immunoprecipitation, Activator, Cdc20 Proteins and Polo kinase. (research.com)

The APC/C is a large E3 ubiquitin ligase, which triggers the metaphase to anaphase transition by marking select proteins for degradation. (wikipedia.org)
Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. (thermofisher.com)
We suggest therefore that hMDC1 functionally regulates the normal metaphase-to-anaphase transition by modulating the Cdc20-dependent activation of the APC/C. (ox.ac.uk)

I am committed to interdisciplinary research, and an example of the successes gained from this approach is the project with Prof A Nabok (Engineering Sheffield Hallam University) using total internal reflection ellipsometry to quantify protein-membrane interactions on native plant membranes and human cell lines. (brookes.ac.uk)
Program access grant to the STFC Harwell Laser Facility 'The Plant Cell Initiative: Protein interactions in the higher plant secretory pathway' 2017-2021 (approximate value of £200K). (brookes.ac.uk)
Using this strain, we studied genetic interactions between cell cycle regulators and CDC20 , and the result was highly consistent with the previously identified interactions. (biomedcentral.com)
The IUPHAR/BPS Guide to PHARMACOLOGY in 2016: towards curated quantitative interactions between 1300 protein targets and 6000 ligands. (idrblab.net)
The degradation timing of APC/C Cdc20 substrates depends on the multi-step nature of ubiquitination, differences in substrate-APC/C Cdc20 interactions, and competition among substrates. (biomedcentral.com)

The following product was used in this experiment: Cdc20 Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-63103, RRID AB_2639559. (thermofisher.com)
Immunohistochemical staining of Cdc20 in human testis and skeletal muscle tissues using Cdc20 Polyclonal Antibody (Product # PA5-63103). (thermofisher.com)
We show that the S1-Fc fusion protein is extraordinarily immunogenic, as evidenced by robust antibody titers noticed by day 7. (genoimmun.com)

These genes, encoding for RNA binding proteins, contain a highly conserved RNA recognition motif and at least one DAZ repeat encoding for a 24 amino acids sequence able to bind other mRNA binding proteins. (ijbs.com)
During growth, genes associated with protein ubiquitination/deubiquitination, cell cycle progression, and proteasomal degradation were affected, while genes linked to protein and carbohydrate catabolism were affected during early development. (frontiersin.org)
Overall, this study reveals the impact of cln5 -deficiency on gene expression in D. discoideum , provides insight on the genes and proteins that play a role in regulating Cln5-dependent processes, and sheds light on the molecular mechanisms underlying CLN5 disease. (frontiersin.org)

CDC20, along with a handful of other Cdc proteins, was discovered in the early 1970s when Hartwell and colleagues made cell-division cycle mutants that failed to complete major events in the cell cycle in the yeast strain S. cerevisiae. (wikipedia.org)

much remains to be learned about how CDC20 binds its substrate. (wikipedia.org)

We show experimentally that the rate of catalysis varies with different substrates of APC/C Cdc20 . (biomedcentral.com)
When two substrates share the same pool of APC/C Cdc20 , their relative enzyme affinities and rates of catalysis influence the partitioning of APC/C Cdc20 among substrates, resulting in substrate competition. (biomedcentral.com)
Depending on how APC/C Cdc20 is partitioned among its substrates, competition can have minor or major effects on the degradation of certain substrates. (biomedcentral.com)

Multiple different mechanisms inhibit Cdks in G1: Cdk inhibitor proteins are expressed, and cyclin gene expression is down-regulated. (wikipedia.org)
The accumulation of both Cyclin and CKI proteins is tightly regulated at the level of transcription. (intechopen.com)
In addition, Cyclin and CKI proteins are controlled at the level of their destruction. (intechopen.com)
Cell cycle regulation is largely based on protein phospho-dephosphorylation events, catalyzed by cyclin dependent kinases (Cdks) and phosphatases (PPases), respectively. (encyclopedia.pub)

this phenotype could be traced back to the CDC20 gene. (wikipedia.org)
If the target protein expression reaches the upper limit, i.e., essential cellular functions come to a halt when the level of protein exceeds a certain limit, the gene/plasmid copy number must decrease to below the upper limit. (biomedcentral.com)
In gTOW, we thus increase the expression of a protein to a limit by increasing the copy number and then determine the upper limit by measuring the corresponding copy number of the gene. (biomedcentral.com)
The commonly used gene-deletion experiments reduce the expression of a target protein to 0. (biomedcentral.com)
To reduce the expression of a gene/protein, we need to reduce the production rate of the transcript, increase the degradation rate of the transcript, decrease the production rate of the protein, or increase the degradation rate of the protein. (biomedcentral.com)

Near its C-terminus it contains seven WD40 repeats, which are multiple short, structural motifs of around 40 amino acids that often play a role in binding with larger protein complexes. (wikipedia.org)
The human CDC20 is about 499 amino acids long, and contains at least four phosphorylation sites near the N-terminus. (wikipedia.org)
These proteins have a highly conserved RNA recognition motif (RRM) for binding target mRNAs and at least one characteristic sequence of 24 amino acids, which are termed as DAZ repeats [ 1 ]. (ijbs.com)

The structure of an enzyme that is bound to a nucleosome - a protein complex around which DNA is wrapped - reveals how contacts between the two orient the enzyme so that it can modify a specific amino-acid residue. (natureasia.com)

The APC/CCdc20 protein complex has two main downstream targets. (wikipedia.org)
Here we identify two critical motifs within BubR1M that contribute to Cdc20 binding and anaphase-promoting complex/cyclosome inhibition: a destruction box (D box) and a phenylalanine-containing motif termed the Phe box.ABubR1 mutant lacking these motifs is defective in MCC maintenance in mitotic human cells but is capable of supporting spindle-checkpoint function. (elsevierpure.com)
The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. (thermofisher.com)

CDC20 appears to act as a regulatory protein interacting with many other proteins at multiple points in the cell cycle. (wikipedia.org)
Recent breakthroughs have uncovered more and more DNA replication licensing machinery proteins (ORC, Cdc6, Cdt1, geminin, etc.) functioning in other cell cycle events, including centrosome replication, mitotic events, transcription and so on. (intechopen.com)
The precise regulations of pre-RC protein levels and assembly are effective ways to prevent reassembly of de novo MCM2-7 onto the replicated origins to re-license and re-replicate the genomic DNA in the subsequent phases of the same cell cycle ( Figure 1) . (intechopen.com)
Our data reveal consistent ENKUR downregulation at both RNA and protein levels across MPN patient specimens and experimental models, with a concomitant upregulation of a cell cycle marker, CDC20. (stanford.edu)
Cell cycle consists of oscillating changes in protein concentrations and activities within the cell allowing for duplication of the host genome and eventual cell division. (pharmafocusasia.com)
Cdc14 is kept sequestered in the nucleolus by its binding to the nucleolar protein Net1 during most of the cell cycle. (encyclopedia.pub)
Progression through the cell cycle is accompanied by dramatic changes in cellular content and behavior, and involves a large number of proteins and processes. (biomedcentral.com)

Stranglehold on the spindle assembly checkpoint: the human papillomavirus E2 protein provokes BUBR1-dependent aneuploidy. (docksci.com)

The two main targets of the APC/C are the S/M cyclins and the protein securin. (wikipedia.org)
Securin is a protein that inhibits separase, which in turn inhibits cohesin, a protein that holds sister chromatids together. (wikipedia.org)
We show experimentally that increased expression of the early APC/C Cdc20 substrate Clb5 does not delay the degradation of the later substrate securin, arguing against a role for competition with Clb5 in establishing securin degradation timing. (biomedcentral.com)
Acute loss of securin and SGO2, but not of either protein individually, resulted in separase deregulation associated with premature cohesin cleavage and cytotoxicity. (nature.com)

I am a plant cell biologist and protein biochemist at Oxford Brookes University with expertise in the structure and function of the plant endoplasmic reticulum (ER), membrane proteins and auxin biosynthesis using biochemical techniques as well as high-resolution live cell imaging. (brookes.ac.uk)
Loss of proteostasis, in itself, is traced to dysregulated mechanisms in protein folding and intracellular calcium signaling at the endoplasmic reticulum (ER). (stanford.edu)

However, like most CLN proteins, the association of CLN5 with a defined biological pathway is still under investigation. (frontiersin.org)
A database to provide information about the known and explored therapeutic protein and nucleic acid targets, the targeted disease, pathway information and the corresponding drugs directed at each of these targets. (idrblab.net)

M-2420 is a fluorescent substrate for β-secretase sites containing Swedish mutations in amyloid precursor protein (APP). (americanchemicalsuppliers.com)
M-2420 is a fluorogenic substrate containing β-secretase site of the Swedish mutation of amyloid precursor protein (APP). (americanchemicalsuppliers.com)

Spermatogenesis produces haploid sperm capable of penetrating the oocyte, whereas oogenesis produces differentiated oocytes that are stockpiled with maternal nutrients, proteins, and mRNAs, and have outer layers that protect the embryo and enable fertilization. (elifesciences.org)
Dazl can regulate the expression, transport and localization of target mRNAs of proteins which control the differentiation, growth and maturation of germ cells. (ijbs.com)

In some invertebrate species, BOULE protein play a pivotal role in germline specification and a conserved regulatory role in meiosis. (ijbs.com)
The APC/C is an E3 ubiquitin ligase that drives mitotic progression by promoting timely degradation of key regulatory proteins [ 3 ]. (biomedcentral.com)

Here I pursued my scientific interests in subcellular protein localisation, bioinformatics, and mathematical modelling. (brookes.ac.uk)

I took up a position at Oxford Brookes University in 2012 investigating the role of reticulon proteins in ER tubulation and viral trafficking in order to develop my international reputation in ER research and advanced imaging. (brookes.ac.uk)
The Human Papillomavirus (HPV) E2 protein, which inhibits the E6 and E7 viral oncogenes, is believed to have antioncogenic properties. (docksci.com)

It has shown that miR-429 could inhibit the development of colorectal cancer via targeting in large tumor suppressor kinase (LATS2), and decreased miR-429 could promote the expression of SRY-box transcription factor 2 (SOX2) and B-cell lymphoma 2 (BCL2) to attenuate the neuronal injury induced by accumulation of amyloid β -protein [ 12 ]. (hindawi.com)

On this research, we examined the immunogenicity of CHO-expressed recombinant SARS-CoV-2 S1-Fc fusion protein in mice, rabbits, and monkeys as a possible candidate for a COVID-19 vaccine . (genoimmun.com)
Description: Candida Albicans (strain SC5314 / ATCC MYA-2876) Hsp90 co-chaperone Cdc37, recombinant protein. (genoimmun.com)

This is integral to the whole process, and there is much to think about in the future by way of things like spindle checkpoint signaling, as well as the contribution of other proteins that play a role in the process. (prospecbio.com)

This process is carried out by the human spliceosome machinery, in which over 300 proteins sequentially assemble with uridine-rich small nuclear RNA molecules (U snRNAs) to form distinct small nuclear ribonucleoprotein complexes (snRNPs). (biomedcentral.com)

Fertilization also leads to changes in mRNA translation and protein stability, which support a period of development driven off of maternal stockpiles. (elifesciences.org)
The localization of DAZ family proteins suggests that they can regulate mRNA translation occurring in the cytoplasm. (ijbs.com)

I further investigated membrane proteins and the targeting of tail-anchored proteins at Sheffield Hallam University. (brookes.ac.uk)

ASO candidates showing significant exon skipping were assessed for their W1282X allele selectivity and the increase of CFTR protein maturation and function. (bvsalud.org)

This phosphorylation occurs in the active site of the plexin GTPase-activating protein (GAP) domain, which in turn inhibits endogenous GAP activity toward Ras/Rap family small GTP-binding proteins by recruiting the phospho-serine/threonine-binding protein 14-3-3ε. (stanford.edu)

Impaired protein homeostasis, though well established in age-related disorders, has been linked in recent research with the pathogenesis of myeloproliferative neoplasms (MPNs). (stanford.edu)

Characterization of PTEN protein showed that it is a phosphatase that acts on proteins and on 3-phosphorylated phosphoinositides, and can therefore modulate signaltransduction pathways that involve lipid second messengers. (neuromics.com)

CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. (thermofisher.com)

Anatomy28

Organisms8

Diseases1

Chemicals and Drugs137

Analytical, Diagnostic and Therapeutic Techniques and Equipment17

Phenomena and Processes65

Anthropology, Education, Sociology and Social Phenomena1

Information Science3

Health Care3

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Cdh19

Mad27

Subunit2

Mitosis6

Interaction11

Homolog1

Activator1

Metaphase3

Interactions5

Antibody3

Genes3

Yeast1

Binds1

Substrates3

Cyclin4

Gene5

Amino acids3

Enzyme1

Complex3

Cell cycle7

BubR11

Securin4

Endoplasmic reticulum2

Pathway2

Substrate2

MRNAs2

Regulatory2

Bioinformatics1

Viral2

Accumulation1

Recombinant2

Spindle1

Complexes1

MRNA translation2

Membrane1

Maturation1

Inhibits1

Homeostasis1

Phosphatase1

Differentiation1